Phcbi MLR-352 Series User manual

MLR-352
MLR-352HSeries
MLR-352H
Please read the operating instructions carefully before using this product, and keep the operating
instructions for future use.
See page 65 for model number.

Operating Instructions
Versatile Environmental Test Chamber

1
CONTENTS
INTRODUCTION ............................................................................................................ 3
PRECAUTIONS FOR SAFE OPERATION .................................................................... 4
ENVIRONMENTAL CONDITIONS ................................................................................. 8
CHAMBER COMPONENTS........................................................................................... 9
Switch box ............................................................................................................... 12
Control panel ........................................................................................................... 13
Remote record terminal........................................................................................... 14
Remote alarm terminal............................................................................................ 15
INSTALLATION SITE ................................................................................................... 16
Caution for installation environment........................................................................ 17
INSTALLATION ............................................................................................................ 18
BEFORE COMMENCING OPERATION
Installing inner attachments .................................................................................... 20
PREVENTING CONTAMINATION ............................................................................. . 21
TOP SCREEN .............................................................................................................. 22
FUNCTIONS THROUGH CONTROL PANEL .............................................................. 23
STANBY OPERATION (MENU/Std-by)........................................................................ 24
SETTING OF KEY LOCK (MENU/Std-by)
Setting of Key Lock (Key Lock) ............................................................................... 25
Setting of Key Unlock (Key Unlock) ........................................................................ 26
HIGH LIMIT/LOW LIMIT ALARM (MENU/Std-by) ........................................................ 27
PROGRAMMING (MENU/Edit) .................................................................................... 28
EDIT OF SAVED PROGRAM (MENU/Edit) ................................................................. 34
START OF PROGRAM (MENU/Run)........................................................................... 35
JOIN FUNCTION .......................................................................................................... 37
SKIP OF STEP (MENU/Skip) ....................................................................................... 38
STOP OF PROGRAM (MENU/Stop)............................................................................ 39
AUTOMATIC DEFROST (MENU/Tools/Date Time)..................................................... 40
MANUAL DEFROST (MENU/M.def) ............................................................................ 41
VARIOUS SETTING (MENU/Tools) ............................................................................. 42
Display of log (Tools/Log) ....................................................................................... 42
Setting of date, time, log (Tools/Date Time) ........................................................... 44
Alarm setting (Tools/Alarm Setting) ........................................................................ 45
Key lock password setting (Tools/Key Lock PW Setting) ....................................... 46
Default setting (Tools/Default Setting) .................................................................... 47
Delete of program (Tools/Delete User Data) .......................................................... 48
LIGHT STEP (ILLUMINANCE SETTING) .................................................................... 50
HUMIDITY CONTROL (MLR-352H ONLY).................................................................. 51

2
CONTENTS
ROUTINE MAINTENANCE .......................................................................................... 52
Cleaning of unit ....................................................................................................... 52
Replacement of fluorescent lamp............................................................................ 52
Cleaning of evaporating tray ................................................................................... 53
Cleaning of filter ...................................................................................................... 53
ALARMS & SAFETY FUNCTIONS
Automatic set temperature alarm ............................................................................ 54
Automatic set humidity alarm (MLR-352H only) ..................................................... 54
Safety functions....................................................................................................... 54
Operation after power failure................................................................................... 54
TROUBLESHOOTING.................................................................................................. 57
DISPOSAL OF UNIT .................................................................................................... 58
PERFORMANCE DATA (MLR-352H) .......................................................................... 63
SPECIFICATIONS........................................................................................................ 64
PERFORMANCE.......................................................................................................... 65
SAFETY CHECKSHEET .............................................................................................. 66

3
INTRODUCTION
■Read the operating instructions carefully before using the Product and follow the instructions for safety
operation.
■PHC Corporation disavows any responsibility for safety if the Product is used for other than the
intended use or used with any procedures other than those given in the operating instructions.
■Keep the operating instructions in a suitable place so that it can be referred to as necessary.
■The contents of the operating instructions are subject to change without notice for improvement of
performance or functions.
■Contact our sales representative or agent if any page of the operating instructions is lost or the page
order is incorrect.
■Contact our sales representative or agent if any point in the operating instructions is unclear or if there
are any inaccuracies.
■No part of the operating instructions may be reproduced in any form without the expressed written
permission of PHC Corporation.
IMPORTANT NOTICE
PHC Corporation guarantees this product under certain warranty conditions. However, please note that
PHC Corporation shall not be responsible for any loss or damage to the contents of the product.
<Intended Use>
This product is a laboratory equipment intended to carry out culture of plant cells/ tissues/organs,
acclimatization/growth of plants, breeding/growth of insects, photomorphogenesis/photosynthesis, and
environmental tests.

4
PRECAUTIONS FOR SAFE OPERATION
It is imperative that the user complies with the operating instructions
as it contains important safety advice.
Items and procedures are described so that you can use this unit correctly and safely.
If the precautions advised are followed, this will prevent possible injury to the user and
any other person.
Precautions are illustrated in the following way:
WARNING
Failure to observe WARNING signs could result in a hazard to personnel
possibly resulting in serious injury or death.
CAUTION
Failure to observe CAUTION signs could result in injury to personnel and
damage to the unit and associated property.
Symbol shows;
T
his symbol means caution.
T
his symbol means an action is prohibited.
T
his symbol means an instruction must be followed.
Be sure to keep the operating instructions in a place accessible to users of this unit.
< Label on the unit >
This mark is labeled on the cover in which the electrical components of high voltage
are enclosed to prevent the electric shock.
The cover should be removed by a qualified engineer or service personnel only.
This symbol indicates a hot surface.

5
USA Only: This product has a fluorescent lamp that contains mercury. Disposal may be regulated in
your community due to environmental considerations. For disposal or information, please visit PHC
website: https://www.phchd.com.
Contains mercury / Contenu avec mercure
For more information on safe handling
procedures, the measures to be taken in
case of accidental breakage and safe
disposal options visit:
ec.gc.ca/mercure-mercury/.
Dispose of or recycle in accordance with
applicable laws.
Pour plus de renseignements sur les
procédures de manutention sécuritaire, les
mesures à prendre en cas de bris accidentel et
les options d’élimination sécuritaire visitez:
ec.gc.ca/mercure-mercury/.
Mettez au rebut ou recyclez conformément
aux lois applicables.
For the State of California, USA Only:
This product contains a CR Coin Cell Lithium Battery which contains Perchlorate Material – special
handling may apply. See www.dtsc.ca.gov/hazardouswaste/perchlorate.

6
PRECAUTIONS FOR SAFE OPERATION
Do not use the unit outdoors. Current leakage or electric shock may result if the unit is exposed to
rain water.
Only qualified engineers or service personnel should install the unit. The installation by
unqualified personnel may cause electric shock or fire.
Install the unit on a sturdy floor and take an adequate precaution to prevent the unit from
turning over. If the floor is not strong enough or the installation site is not adequate, this may result
in injury from the unit falling or tipping over.
Never install the unit in a humid place or a place where it is likely to be splashed by water.
Deterioration of the insulation may result which could cause current leakage or electric shock.
Never install the unit in a flammable or volatile location. This may cause explosion or fire.
Never install the unit where acid or corrosive gases are present as current leakage or electric
shock may result due to corrosion.
Always ground (earth) the unit to prevent electric shock. If the power supply outlet is not
grounded, it will be necessary to install a ground by qualified engineers.
Never ground the unit through a gas pipe, water main, telephone line or lightning rod. Such
grounding may cause electric shock in the case of an incomplete circuit.
Connect the unit to a power source as indicated on the rating label attached to the unit. Use
of any other voltage or frequency other than that on the rating label may cause fire or electric shock.
Never store volatile or flammable substances in this unit if the container cannot be sealed. These
may cause explosion or fire.
Do not insert metal objects such as a pin or a wire into any vent, gap or any outlet on the unit.
This may cause electric shock or injury by accidental contact with moving parts.
Use this unit in safe area when treating the poison, harmful or radiate articles. Improper use
may cause bad effect on your health or environment.
Turn off the power switch (if provided) and disconnect the power supply to the unit prior to any
repair or maintenance of the unit in order to prevent electric shock or injury.
Do not touch any electrical parts (such as power supply plug) or operate switches with a wet
hand. This may cause electric shock.
WARNING

7
Ensure you do not inhale or consume medication or aerosols from around the unit at the time of
maintenance. These may be harmful to your health.
Never splash water directly onto the unit as this may cause electric shock or short circuit.
Never put containers with liquid on the unit as this may cause electric shock or short circuit when
the liquid is spilled.
Never bind, process, or step on the power supply cord, or never damage or break the power
supply plug. A broken power supply cord or plug may cause fire or electric shock.
Do not use the power supply cord if its power supply plug is loose. Such power supply cord
may cause fire or electric shock.
Never disassemble, repair, or modify the unit yourself. Any such work carried out by an
unauthorized person may result in fire, or electric shock or injury due to a malfunction.
Disconnect the power supply plug if there is something wrong with the unit. Continued
abnormal operation may cause electric shock or fire.
When removing the power supply plug from the power supply outlet, grip the power supply
plug, not the cord. Pulling the power supply cord may result in electric shock or fire by short circuit.
Disconnect the power supply plug before moving the unit. Take care not to damage the power
supply cord. A damaged power supply cord may cause electric shock or fire.
Disconnect the power supply plug when the unit is not used for long periods. Keeping the
connection may cause electric shock, current leakage, or fire due to the deterioration of insulation.
If the unit is to be stored unused in an unsupervised area for an extended period, ensure that
children do not have access and that doors cannot be closed completely.
The disposal of the unit should be accomplished by appropriate personnel. Remove doors to
prevent accidents such as suffocation.
Do not put the packing plastic bag within reach of children as suffocation may result.
Do not position this unit and the other unit so that it is difficult to operate the disconnection of
the power supply plug. Failure to disconnect the power supply plug may cause fire if there is
something wrong with the unit.
WARNING

8
PRECAUTIONS FOR SAFE OPERATION
This unit must be plugged into a dedicated circuit protected by branch circuit breaker.
Use a dedicated power source as indicated on the rating label attached to the unit. A multiple-tap
may cause fire resulting from abnormal heating.
Never store corrosive substances such as acid or alkali in this unit if the container cannot be
sealed. These may cause corrosion of inner components or electric parts.
Check the setting when starting up of operation after power failure or turning off of power
switch. The stored items may be damaged due to the change of setting.
Be careful not to tip over the unit during movement to prevent damage or injury.
Prepare a safety check sheet (copy the last page) when you request any repair or maintenance for
the safety of service personnel.
Need to put a safety device to the heat load side by a user when any apparatus is used in the
chamber of this unit.
ENVIRONMENTAL CONDITIONS
This equipment is designed to be safe at least under the following conditions (based on the IEC 61010-1):
■Indoor use;
■Altitude up to 2000 m;
■Temperature 5oC to 40oC;
■Maximum relative humidity 80% for temperature up to 31oC decreasing linearly to 50% relative humidity
at 40oC;
■Mains supply voltage fluctuations up to ±10% of the nominal voltage;
■Transient overvoltages up to the levels of OVERVOLTAGE CATEGORY II;
■Temporary OVERVOLTAGES occurring on the mains supply;
■Applicable pollution degree of the intended environment (POLLUTION DEGREE 2 in most cases);
CAUTION

9
CHAMBER COMPONENTS
1. Control panel:
The control panel is used for setting temperature, humidity (MLR-352H only), light program and alarm.
For the details, see page 13.
2. Door:
5 fluorescent lamps and 5 glow starters are attached inside the door. When the door is closed, it will be
attached firmly to the body by a magnetic gasket.
WARNING
These fluorescent lamps are directly incorporated inside the door and side doors without cover. Do not
damage the fluorescent lamps when open or close the doors and replace the fluorescent lamps.
3. Leveling foot:
To secure the unit, turn the leveling feet beside the casters counterclockwise until they rest securely on
the floor.
4. Caster:
The 4 casters are used to move the unit. When installing the unit, suspend the front two casters using a
leveling foot.
2
1
3
4

10
CHAMBER COMPONENTS
12
7
Air intake vent
15
6
13
16
8
Frost check window
14
9
5
Air exhaust vent
10, 11

11
5. Side door: 5 fluorescent lamps and 5 glow starters are mounted inside of the side doors (right and
left). Open the door to replace fluorescent lamps or glow starters.
6. Switch box: Refer to page 12.
7. Door switch: When the door is opened, the air circulation fan stops to minimize the leakage of cool air.
8. Remote alarm terminal (Back side of the unit): Refer to page 15.
9. Upper shelves (4 pcs): The 4 shelves other than the fifth one at the bottom. The shelf position can
be adjusted vertically. Refer to page 20.
10. Bottom Shelf (1 piece): The fifth shelf at the bottom.
Make sure to attach the air exhaust vent cover to the bottom
shelf before using. Refer to page 20.
11. Air exhaust vent cover: Refer to page 20.
12. Inner door:
This glass inner door minimizes the leakage of cool air when
the cabinet door is opened. The loss of cool air may cause
fluctuation of chamber temperature.
Note: Be careful not to break the glass.
13. Humidifying duct (MLR-352H only): Refer to page 20
14. Filter: Refer to page 53.
15. Evaporating tray:
The evaporating tray collects drain water and allows it to be
evaporated. Before starting the unit, set the evaporating tray
on the rail, which extends from the left side of the frame at the
bottom. (Fig. 1)
Note: Improper setting may result in spillage of water. In
that case, wipe off water.
16. Power switch with circuit breaker:
This switch is for all electric sources. When the operation of
the unit is stopped by this breaker, contact our sales
representative or agent after disconnecting the power supply
plug.
17. Cap (MLR-352 only): Always keep this cap on (Fig. 2).
18. Water supply inlet (MLR-352H only): Refer to page 19.
19. Water supply tank (MLR-352H only): Refer to page 19.
15 Fig. 1
18
19 Fig. 3
17
(
MLR-352
)
(MLR-352H)
Fig. 2
Drain hose
Drain hoses

12
CHAMBER COMPONENTS
Switch box
Inside the switch box, there are the access port and the remote record terminal.
1. Access port
When an instrument that requires a measuring cable and power cord is placed inside the cabinet, the
cable and cord can be led through this access port. When a cord is led through, use the cap to prevent
air from coming in or out.
CAUTION
Always plug the access port with the cap. Failure to plug the port may disturb the refrigerating
performance or cause condensation outside the port.
2. Remote record terminal
Refer to “Remote record terminal” on page 14 for usage.
2
1

13
Control panel
1. LCD panel
2. High limit temperature alarm volume (HIGH LIMIT)
To set the temperature of high limit temperature alarm.
3. Low limit temperature alarm volume (LOW LIMIT)
To set the temperature of low limit temperature alarm.
4. Menu button (MENU)
To open the menu window.
5. LCD contrast adjusting knob
To adjust the contrast of graphic LCD.
6. Alarm buzzer stop key (BUZZER)
To silence the alarm buzzer temporarily.
7. Clear key (CE)
To clear the input value during editing of program.
8. Shift key (Upward, downward, rightward, leftward)
To move the cursor on the LCD panel.
9. Enter key (ENTER)
To determine the selection of menu. When editing the program, press this key to move to the next
article.
10. Character input key
1 2 3 4
5 6 7
8
9
10

14
CHAMBER COMPONENTS
Remote record terminal
The terminal output of remote recorder is temperature(oC) 0 mV~100 mV, light step(LS) 0 mV~100 mV,
relative humidity(%R.H.) 0 mV~100 mV. Please refer below for each control element.
Terminal
No.1 Common (-)
No.2 Temperature (oC) (+)
No.3 Light step (LS) (+)
No.4 Relative humidity (%R.H.) (+)
Range Output
Temperature 0 oC~50 oC 2 mV / oC
Light step 0LS~5LS 20 mV / LS
Relative humidity 0 %~100 % 1 mV / %R.H.
(Example)
Output of temperature 37 oC : 37 oC x 2 mV / oC = 74 mV
Output of light step : 3LS x 20 mV / LS = 60 mV
Output of relative humidity 80 %R.H. : 80 %R.H. x 1 mV / %R.H. = 80 mV
COMMON
GROUND
Temperature
0
oC : 0 mV
50 oC : 100 mV
2 mV/oC
Light step
0LS : 0 mV
5LS : 100 mV
20 mV/LS
Relative humidity
0%R.H.: 0mV
100%R.H. : 100mV
1 mV/%R.H.

15
Remote alarm terminal
The terminal for the remote alarm is located in the data input/output port at the rear top of the frame
(Refer to the figure below). To access the terminal, remove four screws on the rear frame by a screw
driver and take out a cover.
The remote alarm terminal is a contact output. Contact capacity is or 2 A (DC 30 V).
1) Output: Normal open, abnormal close; connect to C and N.O.
2) Output: Normal close, abnormal open; connect to C and N.C.
Power failure: C and N.O. terminal are closed.

16
INSTALLATION SITE
To operate this unit properly and to obtain maximum performance, install the unit in a location with the
following conditions:
■A location not subjected to direct sunlight
Do not install the unit under direct sunlight. Installation in a location subjected to direct sunlight cannot
obtain the intended performance.
■A location with adequate ventilation
Leave at least 10 cm around the unit for ventilation. Poor ventilation will result in a reduction of the
performance and consequently the failure.
■A location away from heat generating sources
Avoid installing the unit near heat-emitting appliances such as a heater or a boiler etc. Heat can
decrease the intended performance of the unit.
■A location with little temperature change
Install the unit under stable ambient temperature. The allowable ambient temperature is between 5 oC
and 35 oC.
■A location with a sturdy and level floor
Always install the unit on a sturdy and level floor. The uneven floor or tilted installation may cause failure
or injury. Install the unit in stable condition to avoid the vibration or noise. Unstable condition may
cause vibration or noise.
WARNING
Install the unit on a sturdy floor. If the floor is not strong enough or the installation site is not
adequate, this may result in injury from the unit falling or tipping over.
Select a level and sturdy floor for installation. This precaution will prevent the unit from tipping.
Improper installation may result in water spillage or injury from the unit tipping over.
■A location not prone to high humidity
Install the unit in the ambient of 80% R.H. or less humidity. Installation under high humidity may cause
current leakage or electric shock.
WARNING
Do not use the unit outdoors. Current leakage or electric shock may result if the unit is exposed to
rain water.
Never install the unit in a humid place or a place where it is likely to be splashed by water.
Deterioration of the insulation may result which could cause current leakage or electric shock.
■A location without flammable or corrosive gas
Never install the unit in a flammable or volatile location. This may cause explosion or fire or may result
in the current leakage or electric shock by the corrosion of the electrical components.
■A location without the possibility of anything fall
Avoid installing the unit in the location where anything can fall down onto the unit. This may cause the
breakdown or failure of the unit.

17
Caution for installation environment
■Suitable temperature range
The acceptable ambient temperature range for this chamber is 5 o C to 35 oC. Avoid operating the
chamber with an ambient temperature lower than 5 oC. This may cause failure by freezing.
■Location convenient for the water supply/drain (MLR-352H only)
MLR-352H needs water supply and drain. Select the location for easy access to supply/drain provision.
■Caution for frost (In the case of pattern 1 for the automatic defrost.)
Operating the chamber with chamber temperature of lower than 10 oC (15 oC for MLR-352H),
accumulates frost on the evaporator. The cooling capacity is degraded and the chamber temperature
rises when the evaporator is clogged by frost. Start the manual defrost when a lot of frost between the
cooling fins is found through the frost check window. For the manual defrost, see page 41. The frost is
formed in short time when the article including much moisture is stored.

18
INSTALLATION
1. Removing the packaging materials and tapes
Remove all transportation packaging materials and tapes. Open the doors and ventilate the unit. If the
outside panels are dirty, clean them with a diluted neutral dishwashing detergent. (Undiluted detergent
can damage the plastic components. For the dilution, refer to the instruction of the detergent.) After
the cleaning with the diluted detergent, always wipe it off with a wet cloth. Then wipe off the panels with
a dry cloth.
Note:
Remove the cable tie banding the power supply cord. Prolonged banding may cause the corrosion of
the cord coating.
2. Adjusting the leveling foot
Extend the leveling feet by turning them counterclockwise
to contact with the floor. Ensure the unit is level. See
Fig. 1.
3. Fixing the unit
Two fixtures are attached to the rear of the frame. See
Fig. 2. Fix the frame to the wall with these fixtures and
rope or chain.
4. Ground (earth)
The ground (earth) is for preventing the electric shock in
the case of the electrical insulation is somehow degraded.
Always ground the unit at the time of installation.
WARNING
Use a power supply outlet with ground (earth) to prevent electric shock. If the power supply outlet is
not grounded, it is necessary to install a ground by qualified engineers.
Never ground the unit through a gas pipe, water main, telephone line or lightning rod. Such
grounding may cause electric shock in the case of an incomplete circuit.
WARNING
In case of being oblige to install the unit near a watery or humid location, consult sales or representative
or agents so that need to set a earth leakage breaker. Earth leakage may cause electric shock. (Use a
specified earth leakage breaker.)
WARNING
Do not put the packing plastic bag within reach of children as suffocation may result.
Fig. 2
Fixture
Fig. 1
Leveling foot