Phcbi MCO-170MP User manual

MCO-170MP
Please read the operating instructions carefully before using this product, and keep the operating
instructions for future use.
See page 101 for model number.
Operating Instructions
O2/CO2 Incubator

1
CONTENTS
INTRODUCTION ........................................................................................... 3
PRECAUTIONS FOR SAFE OPERATION .................................................... 4
LABELS ON INCUBATOR ............................................................................. 9
ENVIRONMENTAL CONDITIONS ................................................................. 9
INCUBATOR COMPONENTS
Unit ........................................................................................................... 10
LCD touch panel ....................................................................................... 12
Remote alarm terminal ............................................................................. 15
INSTALLATION
Installation site .......................................................................................... 16
Installation ................................................................................................ 18
Connecting CO2 gas cylinder ................................................................... 22
Connecting N2/O2 gas cylinder ................................................................. 23
BEFORE COMMENCING OPERATION
Initial cleaning method ............................................................................. 25
Removing inner attachments .................................................................... 26
Installing inner attachments ...................................................................... 29
Filling humidifying pan .............................................................................. 30
FOR BETTER CULTIVATION
Precautions for cultures ........................................................................... 31
Preventing contamination ......................................................................... 32
CORRECT OPERATION ............................................................................. 33
BASIC OPERATION ON LCD TOUCH PANEL ........................................... 34
BASIC PARAMETERS
Numerical input to input window .............................................................. 36
Setting temperature, CO2 density, O2 density
and high limit temperature alarm .................... 37
Setting key lock ........................................................................................ 39
Removing key lock ................................................................................... 41
Automatic N2 gas (O2 gas) cylinder changeover ................................. 43
Manual N2 gas (O2 gas) supply line A/B changeover ............................... 45
ALARM PARAMETERS ............................................................................... 46
OPERATION/ALARM LOG
Setting log interval .................................................................................... 49
Displaying operation log ........................................................................... 50
Exporting operation log ............................................................................ 54
Displaying alarm log ................................................................................. 57
Exporting alarm log .................................................................................. 59
OTHER PARAMETERS
Setting date and time ............................................................................... 62
Setting brightness and sleep .................................................................... 63

2
CONTENTS
UV LAMP PARAMETERS ............................................................................ 65
Using UV lamp ......................................................................................... 65
Setting UV lamp ON period ...................................................................... 66
Lighting UV lamp for 24 hours .................................................................. 68
H2O2 DECONTAMINATION ......................................................................... 70
H
2O2 decontamination .............................................................................. 71
Precautions when handling H2O2 reagent ................................................ 75
Using unlock key ...................................................................................... 75
ELECTRIC LOCK (OPTION) ....................................................................... 76
Setting User-ID ......................................................................................... 76
Setting auto lock ....................................................................................... 78
Using unlock key ...................................................................................... 81
Removing auto lock .................................................................................. 82
GAS AUTO CHANGER (OPTION) .............................................................. 83
Connecting CO2 gas cylinder ................................................................... 83
Automatic CO2 gas supply line changeover ............................................. 84
Manual CO2 gas supply line A/B changeover .......................................... 86
STD GAS AUTO CALIBRATION KIT (OPTION) .......................................... 87
ROUTINE MAINTENANCE ......................................................................... 90
ALARMS, SAFETY, AND SELF-DIAGNOSIS .............................................. 91
TROUBLESHOOTING ................................................................................. 95
DISPOSAL OF UNIT .................................................................................... 98
SPECIFICATIONS ....................................................................................... 99
PERFORMANCE ....................................................................................... 101
SAFETY CHECK SHEET .......................................................................... 102

3
INTRODUCTION
■ Read the operating instructions carefully before using the Product and follow the instructions for safety
operation.
■ PHC Corporation disavows any responsibility for safety if the Product is used for other than the intended
use or used with any procedures other than those given in the operating instructions.
■ Keep the operating instructions in a suitable place so that it can be referred to as necessary.
■ The contents of the operating instructions are subject to change without notice for improvement of
performance or functions.
■ Contact our sales representative or agent if any page of the operating instructions is lost or the page
order is incorrect.
■ Contact our sales representative or agent if any point in the operating instructions is unclear or if there
are any inaccuracies.
■ No part of the operating instructions may be reproduced in any form without the expressed written
permission of PHC Corporation.
IMPORTANT NOTICE
PHC Corporation guarantees this product under certain warranty conditions. However, please note that
PHC Corporation shall not be responsible for any loss or damage to the contents of the product.
<Intended Use>
This equipment is designed for providing an environment with controlled temperature, CO2, O2, N2 and
elevated humidity for the development of ova or embryos at or near physiological conditions.

4
PRECAUTIONS FOR SAFE OPERATION
It is imperative that the user complies with the operating instructions
as it contains important safety advice.
Items and procedures are described so that you can use this unit correctly and safely. If
the precautions advised are followed, this will prevent possible injury to the user and any
other person.
Precautions are illustrated in the following way:
WARNING
Failure to observe WARNING signs could result in a hazard to personnel
possibly resulting in serious injury or death.
CAUTION
Failure to observe CAUTION signs could result in injury to personnel and
damage to the unit and associated property.
Symbol shows;
This symbol means caution.
T
his symbol means an action is prohibited.
T
his symbol means an instruction must be followed.
Be sure to keep the operating instructions in a place accessible to users of this unit.
WARNING
As with any equipment that uses CO2 gas, there is a likelihood of oxygen depletion in the vicinity
of the equipment. It is important that you assess the work site to ensure there is suitable and
sufficient ventilation. If restricted ventilation is suspected, then other methods of ensuring a
safe environment must be considered. These may include atmosphere monitoring and warning
devices.

5
USA Only (Model with a lamp): This product has a lamp that contains mercury. Disposal may be
regulated in your community due to environmental considerations. For disposal or information, please visit
PHC website: https://www.phchd.com .
Contains mercur
y
/ Contenu avec mercure
For more information on safe handling
procedures, the measures to be taken in
case of accidental breakage and safe
disposal options visit:
ec.gc.ca/mercure-mercury/.
Dispose of or recycle in accordance with
applicable laws.
Pour plus de renseignements sur les
procédures de manutention sécuritaire, les
mesures à prendre en cas de bris accidentel et
les options d’élimination sécuritaire visitez:
ec.gc.ca/mercure-mercury/.
Mettez au rebut ou recyclez conformément
aux lois applicables.
For the State of California, USA Only:
This product contains a CR Coin Cell Lithium Battery which contains Perchlorate Material – special
handling may apply. See www.dtsc.ca.gov/hazardouswaste/perchlorate.

6
PRECAUTIONS FOR SAFE OPERATION
Do not use the unit outdoors. Current leakage or electric shock may result if the unit is exposed to
rain water.
Only qualified engineers or service personnel should install the unit. The installation by
unqualified personnel may cause electric shock or fire.
Install the unit on a sturdy floor and take an adequate precaution to prevent the unit from
turning over. If the floor is not strong enough or the installation site is not adequate, this may result in
injury from the unit falling or tipping over.
Never install the unit in a humid place or a place where it is likely to be splashed by water.
Deterioration of the insulation may result which could cause current leakage or electric shock.
Never install the unit in a flammable or volatile location. This may cause explosion or fire.
Never install the unit where acid or corrosive gases are present as current leakage or electric
shock may result due to corrosion.
Always ground (earth) the unit to prevent electric shock. If the power supply outlet is not
grounded, it will be necessary to install a ground by qualified engineers.
Never ground the unit through a gas pipe, water main, telephone line or lightning rod. Such
grounding may cause electric shock in the case of an incomplete circuit.
Connect the unit to a power source as indicated on the rating label attached to the unit. Use of
any other voltage or frequency other than that on the rating label may cause fire or electric shock.
Never store volatile or flammable substances in this unit if the container cannot be sealed. These
may cause explosion or fire.
Do not insert metal objects such as a pin or a wire into any vent, gap or any outlet on the unit.
This may cause electric shock or injury by accidental contact with moving parts.
Use this unit in safe area when treating the poison, harmful or radiate articles. Improper use
may cause bad effect on your health or environment.
Turn off the power switch (if provided) and disconnect the power supply to the unit prior to any
repair or maintenance of the unit in order to prevent electric shock or injury.
Do not touch any electrical parts (such as power supply plug) or operate switches with a wet
hand. This may cause electric shock.
WARNING

7
Ensure you do not inhale or consume medication or aerosols from around the unit at the time of
maintenance. These may be harmful to your health.
Never splash water directly onto the unit as this may cause electric shock or short circuit.
Never put containers with liquid on the unit as this may cause electric shock or short circuit when
the liquid is spilled.
Never bind, process, or step on the power supply cord, or never damage or break the power
supply plug. A broken power supply cord or plug may cause fire or electric shock.
Do not use the power supply cord if its power supply plug is loose. Such power supply cord may
cause fire or electric shock.
Never disassemble, repair, or modify the unit yourself. Any such work carried out by an
unauthorized person may result in fire, or electric shock or injury due to a malfunction.
Disconnect the power supply plug if there is something wrong with the unit. Continued
abnormal operation may cause electric shock or fire.
When removing the power supply plug from the power supply outlet, grip the power supply
plug, not the cord. Pulling the cord may result in electric shock or fire by short circuit.
Do not use the supplied power cord for other electrical equipment. Such power supply cord may
cause fire or electric shock.
Disconnect the power supply plug before moving the unit. Take care not to damage the power
supply cord. A damaged cord may cause electric shock or fire.
Disconnect the power supply plug when the unit is not used for long periods. Keeping the
connection may cause electric shock, current leakage, or fire due to the deterioration of insulation.
If the unit is to be stored unused in an unsupervised area for an extended period, ensure that children
do not have access and that doors cannot be closed completely.
The disposal of the unit should be accomplished by appropriate personnel. Remove doors to
prevent accidents such as suffocation.
Do not put the packing plastic bag within reach of children as suffocation may result.
Use the reagent specified by our company for H2O2 decontamination. Using a different H2O2
solution may result in explosion or damage to the incubator.
When performing H2O2 decontamination, securely close the internal and external doors. Failure to
do so may be harmful to health due to leakage of H2O2 gas.
During H2O2 decontamination, plug the access hole with the silicon cap that is provided. Failure to
do so may be harmful to health due to leakage of H2O2 gas.
WARNING

8
PRECAUTIONS FOR SAFE OPERATION
Always use the removable power supply cord that is provided. Other power supply cord may
cause electric shock or fire.
Do not position this unit and the other unit so that it is difficult to operate the disconnection of
the power supply plug. Failure to disconnect the power supply plug may cause fire if there is
something wrong with the unit.
This unit must be plugged into a dedicated circuit protected by branch circuit breaker.
Use a dedicated power source as indicated on the rating label attached to the unit. A multiple-tap may
cause fire resulting from abnormal heating.
Never store corrosive substances such as acid or alkali in this unit if the container cannot be
sealed. These may cause corrosion of inner components or electric parts.
Check the setting when starting up of operation after power failure or turning off of power
switch. The stored items may be damaged due to the change of setting.
Be careful not to tip over the unit during movement to prevent damage or injury.
Prepare a safety check sheet (copy the last page) when you request any repair or maintenance for
the safety of service personnel.
Wear rubber gloves when handling the H2O2 reagent. Direct contact with the H2O2 reagent may
result in inflammation of the skin.
H2O2 decontamination can be performed only for the chamber and chamber attachments with standard
specifications, and not for any other objects.
Perform H2O2 decontamination with the chamber attachments arranged as specified by our
company. Arranging them in a different way may result in insufficient decontamination.
After H2O2 decontamination has been completed, wear rubber gloves and use a non-woven cloth to
wipe off the residual H2O2 fluid from the bottom of the chamber, any objects that were
decontaminated, and the bottoms of ducts.
Do not touch the hot surface. This may cause burns.
WARNING
CAUTION

9
LABELS ON INCUBATOR
Warning and caution labels are attached to the incubator. The following table describes the labels.
This label is attached to covers that access high-voltage electrical components to
prevent electric shock. Only a qualified engineer or service personnel should be
allowed to open these covers.
This symbol indicates an ultraviolet light (UV) caution.
This symbol indicates that caution is required. Refer to product documentation for
details.
This symbol indicates a hot surface.
This symbol indicates an earth.
This symbol means “ON” for a power switch.
This symbol means “OFF” for a power switch.
ENVIRONMENTAL CONDITIONS
This equipment is designed to be safe at least under the following conditions (based on the IEC 61010-1):
■ Indoor use;
■ Altitude up to 2000 m;
■ Temperature 5 oC to 40 oC;
■ Maximum relative humidity 80 % for temperature up to 31 oC decreasing linearly to 50 % relative
humidity at 40 oC;
■ Mains supply voltage fluctuations up to ±10 % of the nominal voltage;
■ Transient overvoltages up to the levels of OVERVOLTAGE CATEGORY II;
■ Temporary OVERVOLTAGES occurring on the mains supply;
■ Applicable pollution degree of the intended environment (POLLUTION DEGREE 2 in most cases);

10
INCUBATOR COMPONENTS
Unit
1 2 3 4 Tray 5 6
7
8*1
9
10
16
15*
2 Electric key*2 14 13 12 11
*1: When an optional UV system set MCO-170UVS
is installed.
*2: When an optional electric lock MCO-170EL is
installed.
23
(When some inner
attachments are removed)
(When 16 is removed)
Sample air outlet cap
17 18 19*1 20 21 22
Handles
Leveling
feet
Leveling
foot
Lower right side
Removable power
supply cord port
24
25
Access port
cover Silicon cap
Rear side

11
1. Outer door: The outer door is held to the frame with the magnetic seal. The door heater is installed in
the door panel. The door opening is reversible. Contact our sales representative or agent to change the
door hinge from left to right or vice versa.
2. Gastight split door: Made of tempered glass, however avoid excessive impact on the glass.
3. Inner door: Open when take the trays out or clean inside.
4. Tray catches: Insert tray to fit the concave portion on chamber.
5. Duct: The duct for the path for circulating air. It is removable.
6. Fan cover (above the duct): The fan cover serves as the inlet for circulating air. It is removable.
7. Fan: The fan is made from polypropylene resin. It can be disinfected in an autoclave.
8. UV lamp*1: This UV lamp does not generate ozone. Never look directly at the UV light. Refer to page
66~69 for using. For replacement, contact our sales representative or agent.
9. Humidity control bar: Reduces condensation in the unit caused by the external environment and
frequent door openings.
10. Gas injection port: N2 gas (O2 gas) is injected from here under the condition that O2 density control is
ON. For faster recovery of humidity in the chamber, connect the gas injection port and the gas injection
nozzle by the gas injection nozzle tube. Refer to page 18 for installing.
11. Humidifying pan cover: This cover prevents the UV light entering the chamber. Always use it. Using
without it may have a bad influence on the chamber temperature distribution and humidity recovery.
12. Gas injection nozzle: Refer to page 18 for installing.
13. Humidifying pan: Fill the humidifying pan with sterile distilled water, and set the humidifying pan with
the inner side flush against the back. Install the humidifying pan in a longitudinal direction as its shorter
side is placed in the back.
14. Door switch: Detects the door opening/closing and stops the fan, electromagnetic valve for CO2 and
electromagnetic valve for N2/O2 when the door is open. The UV lamp*1 is also deactivated by the door
opening.
15. Key hole*2: This is the hole to unlock with unlock key while outer door is locked by electric lock.
16. Switch cover: Prevent the accident of gas tube disconnected by the unexpected touch or power off.
17. Sample air outlet: The sample air outlet also functions as an internal gas outlet. Normally, cover this
outlet with the sample air outlet cap.
18. Power switch: The power switch of this incubator (ON-“I”, OFF-“O”). It also functions as an
overcurrent breaker.
19. Glow starter*1: The glow is started for the UV lamp.
20. Connecting port A for CO2 gas pipe: Refer to page 22 for gas cylinder connection. Ensure that the
gas pressure is set at 0.03 MPa(G)~0.1 MPa(G) (0.3 kgf/cm2(G)~1 kgf/cm2(G), 4.4 psi(G)~14.5 psi(G)).
Note: When the optional MCO-21GC gas auto changer is installed, both ports A and B are available. Refer
to page 84~86 for gas auto changer.
21. Connecting port A/B for N2/O2 gas pipe: Refer to page 23~24 for gas cylinder connection. Ensure
that the gas pressure is set at 0.05 MPa(G)~0.1 MPa(G) (0.5 kgf/cm2(G)~1 kgf/cm2(G), 7.3 psi(G)~ 14.5
psi(G)).

12
INCUBATOR COMPONENTS
22. Power supply cord cover plate: This plate is to prevent the removable power supply cord being
come off.
23. USB port: Insert USB memory to export operations and alarms log. Refer to page 53~61.
Note: It is impossible to use USB memory which is required password input.
24. Access port: Place the silicon caps on both outside and inside of the port when the port is not being
used.
25. Remote alarm terminals: This terminal informs the alarm to remote location by connecting to external
alarm unit. Refer to page 15.
LCD touch panel
The following display (called the Top screen) will appear when the power switch is turned ON.
Note: It takes approximately 20 seconds until Top screen is displayed. During warming-up, “Status: Gas
sensor initializing” is displayed in the Message display field (18), and “--.-” is displayed in the present CO2
density display field (5) and the present O2 density display field (10).
2 4
3
1
1. Present temperature display field
The current chamber temperature is displayed.
2. Set temperature value display field
The set value of chamber temperature is
displayed. Default setting: 37 oC.
3. Heating indicator
This lamp lights when the heater is energized.
4. Present date/time display field
Normally, this indicator shows date and time. The
date and time is simply set when the incubator is
shipped from the factory. Refer to page 62 for
details.

13
8 9 13 14
7 5 6 12 10 11
5. Present CO2 density display field
The current chamber CO2 density is displayed.
Nothing is displayed when CO2 density is set 0 %.
6. Set CO2 density value display field
The set value of the chamber CO2 density is
displayed. Default setting: 0 %.
7. CO2 gas injection indicator
This lamp lights when CO2 gas is being injected.
8. CO2 gas supply line indicator A and B*1
Current supplying CO2 gas supply line
(connecting port for CO2 gas pipe) is displayed.
After an optional gas auto changer MCO-21GC
changes CO2 gas supply line automatically, the
connecting port A/B for gas pipe that becomes
empty is displayed in reverse video and blinks.
9. CO2 gas supply line select key*1
This is a key to select CO2 gas supply line A or B
(Connecting port A or B for CO2 gas pipe). When
an optional gas auto changer MCO-21GC is
installed, CO2 gas supply line A/B changes over
automatically when CO2 gas cylinder is empty.
Changeover is also workable by pressing this key.
10. Present O2 density display field
The current chamber O2 density is displayed.
Nothing is displayed when O2 density is set 20 %.
11. Set O2 density value display field
The set value of the chamber O2 density is
displayed. Default setting: 20 %.
12. N2/O2 gas injection indicator
This lamp lights when N2 gas (O2 gas) is being
injected.
13. N2/O2 gas supply line indicator A and B
Current supplying N2/O2 gas supply line
(connecting port for N2/O2 gas pipe) is displayed.
After changing N2/O2 gas supply line automatically,
the connecting port A/B for gas pipe that becomes
empty is displayed in reverse video and blinks.
14. N2/O2 gas supply line select key
This is a key to select N2/O2 gas supply line A or B
(Connecting port A or B for N2/O2 gas pipe). N2/O2
gas supply line A/B changes over automatically
when N2 gas cylinder (O2 gas cylinder) is empty.
Changeover is also workable by pressing this key.
*1: Only when an optional component MCO-21GC (Gas auto changer) is installed, this key is workable.
They are not displayed when the MCO-21GC is not installed.

14
INCUBATOR COMPONENTS
15 16 17
18
23 22 21 20
15. Overheat display
High limit temperature alarm is activated:
“Over Heat” is displayed alternately in normal
characters and reverse video.
16. UV lamp condition display
UV lamp ON: “UV: On” is displayed.
UV lamp OFF: “UV: Off” is displayed.
Note: Nothing is displayed when an optional UV
system set MCO-170UVS is not installed to the
MCO-170MP.
17. Outer door (opening/closing) display
Open: “Door: Open” is displayed alternately in
normal characters and reverse video.
Close: “Door: Closed” is displayed.
Locked: “Door: Locked” is displayed.*2
18. Message display field
Alarms, errors or messages are displayed when
fault occurs. Refer to page 91~94.
Note: When there are a number of alarms/errors,
the display shows the message. For example, if 2
alarms/errors occur in total, the display shows
“1/2”.
19. Message select key
When there are a number of alarm/errors, the
message on the screen is changeable.
20. Menu key
Press this key to lead the Menu screen. It is
possible to set various setting on the Menu
screen. Refer to page 34.
*2: Auto lock function by electric lock is workable when an optional electric lock MCO-170EL is installed. If
not, “Door: Locked” or Unlock key are not displayed.
19

15
Remote alarm
terminal
21. H2O2 key*3
This key is to run H2O2 decontamination. Refer
to page 71~75.
22. Unlock key*2
Press this key is to unlock the outer door when
it is auto-locked by electric lock. Refer to page
80. When the auto lock function is OFF, this
key is not displayed.
23. Buzzer key
Press this key to silence the buzzer. However,
when the ring back is ON, the buzzer will
sound again when the ring back passed and
the alarm state still continues. Refer to page
48 and 91~94.
Note: It is not possible to silence the buzzer
for the high limit temperature alarm.
*3: The H2O2 decontamination function is workable when all UV system set MCO-170UVS, H2O2 generator
MCO-HP, H2O2 decon board MCO-170HB and electric lock MCO-170EL are installed in the MCO-170MP.
If not, the H2O2 key is not displayed on the LCD touch panel.
Remote alarm terminal
The alarm of this product can be informed at a remote location from this product by connecting the external
alarm unit to the remote alarm terminals. For the type and behavior of remote alarm output, refer to page
91~94.
The terminal of the remote alarm is installed at the rear
upper right of the unit (See the figure on the point). The
alarm is outputted from this terminal. Contact capacity is
DC 30 V, 2 A.
When Buzzer key is pressed, the behavior of the remote
alarm is showed in Table.1.
Note: In the door alarm, the remote alarm does not work.
Refer to page 91 and 93.
Note: It is recommended to use standard signal and
interface cables with a maximum length of 30 meters.
Table 1 Behavior of the remote alarm when pressing the Buzzer key
Remote Alarm setting
(Refer to page 46~48)
Connecting
terminal
Normal
condition
Abnormal condition
(Including in the cases of power outage and
of where the power suppl
y
plu
g
is pulled out.
)
When pressin
g
the buzzer ke
y
ON:
Non-interlock with Buzzer key
COM.-N.C. Close Open Open
(
Maintain in abnormalit
y)
*
COM.-N.O. Open Close Close
(
Maintain in abnormalit
y)
*
OFF:
Interlock with Buzzer key
COM.-N.C. Close Open Close
(
Return to normal
)
COM.-N.O. Open Close Open
(
Return to normal
)
*In case of Err01 (CO2 gas cylinder empty), Err02 (N2 gas cylinder or O2 gas cylinder empty), Err11 and
12(CO2 sensor error), the condition returns to normal.

16
INSTALLATION
Installation site
For correct operation of the incubator, install it in a location with the following conditions.
WARNING
When using CO2/N2 gas for control, make sure that there is adequate ventilation. Using CO2/N2 gas in a
small room without adequate ventilation may cause gas poisoning or oxygen deprivation. In addition, when
opening the incubator doors, do not directly inhale the air in the chamber.
Si l’appareil est utilisé dans un evdroit restreint, le niveau de la densite CO2/N2 de l’air peut s’élever et peut
être nocif aux humains. Evitez d’aspirer l’air provenant de l’inérieur de l’appareil quand vous ouverz la
porte.
 Normal air environment
Install the incubator in an environment with normal air.
 Do not expose to direct sunlight
Do not install the incubator in a location where it will be exposed to direct sunlight. If the incubator is
operated in direct sunlight, performance will be adversely affected.
 Separate from heat sources
Do not install the incubator near significant heat sources, such as heaters, boilers, ovens, or autoclaves.
Heat will adversely affect the performance of the incubator.
 Ambient temperature at least 5 C lower than set temperature
The control temperature of the incubator is at least 5 C higher than the ambient temperature. For example,
if the chamber is controlled at 37 C, the ambient temperature must be 32 C or less. Do not allow the
ambient temperature to become too high.
 Strong and level floor
Select a site with a strong and level floor. If the floor is uneven or the installation is not level, the incubator
will be unstable and this may cause accident or injury. To avoid vibration and noise, always make sure that
the installation is stable. An unstable surface may result in vibration or noise.
WARNING
Install the incubator at a location that can support the weight. If the floor is not strong enough or if the
installation is insufficient, the incubator may fall over and cause injury.
Always make sure that the floor is strong, even, and level, and that the incubator will not tip over.
A
n insufficient installation may result in injury due to water leakage or the incubator falling over.
 Separate from vibration products
Do not install the incubator near vibration products. Vibration may cause culture failure.

17
 Low humidity
Select a site with a relative humidity of 80 %R.H. or lower. Using the incubator in high humidity may result
in current leakage or electric shock.
WARNING
Do not use the incubator outdoors. If the incubator is exposed to rain water, it may result in current
leakage or electric shock.
Never install the incubator in a moist location, such as near a sink or water line, or where it is likely
to be exposed to water. In addition, do not install it near water or steam pipes. Moisture can cause the
insulation to deteriorate, which may result in current leakage or electric shock.
 No inflammable or corrosive gas
Never install the incubator in a location where it will be exposed to inflammable or corrosive gas. Doing so
may result in explosion or fire. In addition, insulation may deteriorate due to corrosion of protective casing,
resulting in current leakage or electric shock.
 No falling objects
Do not install the incubator in a location where there is the possibility of objects falling from above. Doing
so may result in damage or accident.

18
INSTALLATION
Installation
1. Remove the packing tape and clean up.
Remove all the tapes that are securing the doors and the inner attachments. Open the doors for ventilation.
If the outer panels are dirty, wet a cloth with a diluted neutral detergent and wipe them. (Undiluted
detergent can damage the plastic components. For the dilution, refer to the instruction of the detergent.)
Wipe off the residual detergent with a wet cloth and then wipe off any moisture.
Note: Remove the cable tie banding the power supply cord. Prolonged banding may cause the corrosion
of the cord coating.
WARNING
Do not leave the plastic wrapping bags within reach of children. If the bag is placed over a child’s
head, it can block the mouth and nose and cause suffocation.
2. Connecting a gas injection nozzle.
In the case of control of O2 density in the chamber, connect the gas injection nozzle to the gas injection
port by using the gas injection nozzle tube enclosed (inner diameter; 5 mm, outer diameter; 9 mm, length;
210 mm) (Fig. 1). This helps faster recovery of humidity after opening of the inner door.
Fig.1
Gas injection port
Gas injection nozzle tube
Gas injection nozzle