Promega GloMax 96 User manual

Powering the Luminometer
1. Remove the plastic packing material that holds the optical head in place during shipping.
2. Connect the power supply to the GloMax™ 96 Microplate Luminometer.
3. Connect the 9-pin serial cable to the luminometer and the computer.
4. Turn “ON” both the luminometer and your computer.
5. Install the GloMax™ Software provided on the CD-ROM that came with the luminometer.
Starting the Software
6. Double-click on the GloMax™ icon on your computer desktop to start the software.
7. Select “Run Promega Protocol” if you are running a Promega assay. A list of Promega protocols will appear. Select the
one that is appropriate for your assay. Alternatively, select “Create New Protocol” if you wish to write a new protocol, or
select “Open Saved Protocol” if you wish open an existing protocol that you saved previously.
Setting the Options on a New Protocol
8. When you choose “Create New Protocol”, a wizard tool will guide you through the setup.
9. First, select the number of injectors needed: 0, 1 or 2.
10. Use the arrow keys to change each setting from the default vaule. Click the “Accept” button to accept one setting and
proceed to the next parameter. To readjust a setting, click “Reset values for these parameters.”
For
noninjection protocols, you may choose to set a delay time before the luminometer begins reading the microplate.
You may also adjust the integration time per well, the number of repeated runs and the delay between repeated runs.
For
injection protocols, you may choose to measure your samples before the injection. You may also set the injection
volume for each injector and the delay between injection and measurement. For all measurements, you may adjust the
integration time per well.
11. Select the wells you wish to read. You can select or deselect the entire plate by clicking “All”. You can select or deselect
individual rows and columns by clicking on the letter or number identifying the row or column. Click on an individual well
to select or deselect that well.
12. Click “Finish” or “Save Protocol as” to store the protocol for future use.
13. To review or change settings, select “Options” from the Main Dialog Box.
•
••
•••
••••
•••••
••••••
Printed in USA. Revised 3/09
Part# 9FB082
© 2006, 2009 Promega Corporation. All Rights Reserved.
ORDERING/TECHNICAL INFORMATION:
www.promega.com • Phone [phone] or 800-356-9526 • Fax [phone]
Quick Start Guide
GloMax™ 96 Microplate Luminometer
INSTRUCTIONS FOR USE OF PRODUCTS E6501, E6511 AND E6521 PROTOCOL
Quick

•
••
•••
••••
•••••
••••••
© 2006, 2009 Promega Corporation. All Rights Reserved.
Prices and specifications subject to change without prior notice.
Printed in USA. Revised 3/09.
Part #9FB082
ORDERING/TECHNICAL INFORMATION:
www.promega.com • Phone [phone] or 800-356-9526 • Fax [phone]
Quick Start Guide
GloMax™ 96 Microplate Luminometer
INSTRUCTIONS FOR USE OF PRODUCTS E6501, E6511 AND E6521 PROTOCOL
Quick
Starting a Run
14. Enter any information that you wish to record in the Experiment, Operator,
Plate Number and Notes fields located in the Main Dialog Box.
15. If you are using injectors, you need to prime the injectors before running the
assay.
Place reagent bottles in Reagent Bottle Holder. Secure the inlet tubing in
the reagent bottle by placing the inlet lines under the Tubing Guide on the
left side of the instrument.
Hold the released tips over a waste container during priming.
Click on the Prime tab. Click on “Injector 1”, “Injector 2” or “Both Injectors” to
select the injectors. Click “Prime” on the follow-up screen to start pumping.
16. Insert your plate into the sample tray and close the sample tray cover. Close
the instrument lid.
17. Click the “Start” button in the Main Dialog Box to start the run. The Excel
spreadsheet should maximize after the first selected row is measured.
18. After the experiment is finished, you may retrieve any unused reagent by
clicking the Reverse Purge tab.
19. Wash injectors. Place the tip or tips in a waste bottle and click the Flush tab.
Select the injectors to be washed. Wash the injectors three times with water.
Next, wash three times with 70% ethanol, and follow with three more washes
with water. Dry the injectors by flushing them with air three times.
NNoottee::Remember to remove sample plate after the experiment is finished.
NNoottee::Do not use bent or damaged injector tips. Check the tips periodically
and replace as needed.
5487MA
Injector
Inlet tubing
Reagent Bottle
Holder
5494MA
Waste container
5486MA
DDoonnoottuse bent or damaged injector tips.
5493MA
Injector tip holder
12
5492MA
Injector tip holder
12
For additional information, see the GloMax™ 96 Microplate Luminometer
Technical Manual #TM278, available online at: wwwwww..pprroommeeggaa..ccoomm