Macherey-Nagel NucleoMag 96 PCR User manual

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52355 Düren · Deutschland
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CH Tel.: [phone] [email protected]
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MACHEREY-NAGEL GmbH & Co. KG
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52355 Düren · Deutschland
Bioanalysis
MACHEREY-NAGEL
User manual
June 2021 / Rev. 04
nNucleoMag®96 PCR
PCR clean-up

Contact MN
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MACHEREY-NAGEL GmbH & Co. KG
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Tel.: [phone]
Toll-free: 0800 26 16 000 (Germany only)
E-mail: [email protected]
Technical Support Bioanalysis
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3MACHEREY-NAGEL – 06/2021, Rev. 04
PCR clean-up
Table of contents
1 Components 4
1.1 Kit contents 4
1.2 Equipment and consumables to be supplied by user 4
1.3 About this user manual 4
2 Product description 5
2.1 The basic principle 5
2.2 Kit specifications 5
2.3 Magnetic separation systems 5
2.4 Adjusting the shaker settings 6
2.5 Handling of beads 6
2.6 Elution procedures 7
3 Storage conditions and preparation of working solutions 8
4 Safety instructions 9
5 Protocol for clean-up of 50 μL PCR reactions 10
6 Appendix 14
6.1 Troubleshooting 14
6.2 Ordering information 15
6.3 Product use restriction / warranty 16

MACHEREY-NAGEL – 06/2021, Rev. 044
PCR clean-up
1 Components
1.1 Kit contents
NucleoMag®96 PCR
REF
1 x 96 preps
744100.1
4 x 96 preps
744100.4
24 x 96 preps
744100.24
NucleoMag®P-Beads 1.4 mL 5.6 mL 33.6 mL
Binding Buffer MP1 20 mL 80 mL 500 mL
Wash Buffer MP2 40 mL 160 mL 1000 mL
Wash Buffer MP3 (Concentrate)* 25 mL 100 mL 2 x 200 mL
Elution Buffer MP4 15 mL 30 mL 250 mL
Elution Plate U-bottom
(including Self-adhering PE Foil)
1 4 24
User manual 1 1 1
1.2 Equipment and consumables to be supplied by user
Product REF Pack of
• Magnetic separation system
e.g., NucleoMag®SEP (see section 2.3)
744900 1
• Separation plate for magnetic beads
separation,
e.g., Square-well Block (96-well block with
2.1 mL square-wells)
740481
740481.24
4
24
1.3 About this user manual
It is recommended to read the instructions of this user manual carefully before use. All
technical literature is also available on the internet at www.mn-net.com.
Please contact Technical Service regarding information about changes of the current user
manual compared to previous or updated revisions.
¹ For preparation of working solutions and storage conditions see section 3.

5MACHEREY-NAGEL – 06/2021, Rev. 04
PCR clean-up
2 Product description
2.1 The basic principle
The NucleoMag®96 PCR procedure is based on reversible adsorption of nucleic acids to
paramagnetic beads under appropriate buffer conditions. Adjusting the PCR reaction to
binding conditions and addition of paramagnetic beads can be carried out simultaneously.
After magnetic separation and removal of supernatant, the beads are washed to remove
contaminants and salt. A short drying step is necessary to remove ethanol from previous
washing steps. Finally, highly purified DNA is eluted with low salt elution buffer or water and
can be used directly for downstream applications. The NucleoMag®96 PCR kit can be used
either manually or automated on standard liquid handling instruments.
2.2 Kit specifications
• NucleoMag®96 PCR is designed for rapid manual and automated clean-up of PCR
fragments using the NucleoMag®SEP Magnetic Separator (see ordering information)
other magnetic separation systems (see section 2.3). Manual processing time for 96
samples is about 45 min.
• NucleoMag®96 PCR is easily adapted to common liquid handling instruments.
The actual processing time and sample volume to be processed depends on the
configuration of your instrument and the used magnetic separation system. Typically, 96
samples can be purified in about 30–45 min.
• The kit provides reagents for the purification of 3–5 μg DNA from 50 μL PCR samples.
• Typical concentration of the purified PCR samples of 75–200 ng/μL can be achieved.
• The purity of recovered PCR products is A260/A280 ≥ 1.7–1.9.
• The kit is designed for use with or without detergent containing PCR buffers.
• Purified PCR products are ready-to-use for downstream applications, like automated
fluorescent sequencing, labeling, microarray analysis, cloning, or restriction digestion.
• NucleoMag®96 PCR can be processed completely at room temperature. Elution at
55 °C will increase the recovery by about 10–15 %.
• NucleoMag®P-Beads are highly reactive, superparamagnetic beads. The binding
capacity is 0.3 μg of DNA per 1 μL of NucleoMag®P-Bead suspension, 1 μL of
suspension contains 150 μg of beads.
2.3 Magnetic separation systems
For use of NucleoMag®96 PCR, the use of the magnetic separator NucleoMag®SEP is
recommended. Separation is carried out in a Square-well Block (see ordering information).
The kit can also be used with other common separators.
Magnetic separator Separation plate or tube
NucleoMag®SEP (MN REF 744900) Square-well Block (MN REF 740481)
Tecan Te-MagS™ 1.5 mL tubes without lid (Sarstedt)

MACHEREY-NAGEL – 06/2021, Rev. 046
PCR clean-up
Static magnetic pins
Separators with static magnetic pins, for example, NucleoMag®SEP (for manual use and for
use on liquid handling workstations): This type of separator is recommended in combination
with a suitable microplate shaker for optimal resuspension of the beads during the washing
and elution steps. Alternatively, beads can be resuspended in the buffer by pipetting up and
down several times. For fully-automated use on liquid handling workstations, a gripper tool is
required, the plate is transferred to the magnetic separator for separation of the beads and
transferred to the shaker module for resuspension of the beads.
Movable magnetic systems
Separators with moving magnetic pins: Magnetic pins/rods are moved from one side of the
well to the other and vice versa. Beads follow this movement and are thus pulled through
the buffer during the wash and elution steps. Separation takes place when the system stops.
Automated separators
Separators with moving magnets: Magnetic beads are transferred into suitable plates or
tubes. Beads are resuspended from the rod-covered magnets. Following binding, washing or
elution beads are collected again with the rod-covered magnets and transferred to the next
plate or tube.
2.4 Adjusting the shaker settings
When using a plate shaker for the washing and elution steps, the speed settings have
to be adjusted carefully for each specific separation plate and shaker to prevent cross-
contamination from well to well. Proceed as follows:
Adjusting shaker speed for binding and wash steps:
• Load 300 μL dyed water (select to the wells of the separation plate. Place the plate on
the shaker and start shaking with a moderate speed setting for 30 seconds. Turn off the
shaker and check the plate surface for small droplets of dyed water.
• Increase speed setting, shake for an additional 30 seconds, and check the plate
surface for droplets again.
• Continue increasing the speed setting until you observe droplets on top of the
separation plate. Reduce speed setting, check again, and use this setting for the
washing step.
Adjusting shaker speed for the elution step:
• Load 100 μL dyed water to the wells of the collection plate and proceed as described
above.
2.5 Handling of beads
Distribution of beads
A homogeneous distribution of the magnetic beads to the individual wells of the separation
plate is essential for a high well-to-well consistency. Therefore, before distributing the beads,
make sure that the beads are completely resuspended. Shake the storage bottle well or
place it on a vortexer shortly. Premixing magnetic beads with the binding buffer allows easier
homogenous distribution of the beads to the individual wells of the separation plate. During

7MACHEREY-NAGEL – 06/2021, Rev. 04
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automation, a premix step before aspirating the beads/binding buffer mixture from the
reservoir is recommended to keep the beads resuspended.
Magnetic separation time
Attraction of the magnetic beads to the magnetic pins depends on the magnetic strength of
the magnetic pins, the selected separation plate, distance of the separation plate from the
magnetic pins, and the volume to be processed. The individual times for complete attraction
of the beads to the magnetic pins should be checked and adjusted on each system. It is
recommended using the separation plates or tubes specified by the supplier of the magnetic
separator.
Washing the beads
Washing the beads can be achieved by shaking or mixing. In contrast to mixing by pipetting
up and down, mixing by shaker or magnetic mixing allows simultaneous mixing of all samples.
This reduces the time and number of tips needed for the preparation. Resuspension by
pipetting up and down, however, is more efficient than mixing by a shaker or magnetic mix.
Method Resuspension
efficiency
Speed Number of tips
needed
Magnetic mix + ++ Low
Shaker ++ ++ Low
Pipetting +++ +* High
+: acceptable, ++: good, +++: excellent, * 8-channel pipetting device
2.6 Elution procedures
Purified DNA can be eluted directly with the supplied Elution Buffer MP4 or water (pH7.5–8.5).
Elution can be carried out in a volume of ≥ 25 μL (per 12 μL bead suspension). It is essential
to cover the NucleoMag®P-Beads completely with elution buffer during the elution step. The
volume of dispensed elution buffer depends on the magnetic separation system (e.g., the
position of the pellet inside the separation plate). For efficient elution, the magnetic bead
pellet should be resuspended completely in the elution buffer. For some separators, high
elution volumes might be necessary to cover the whole pellet.
Elution is possible at room temperature. Yield can be increased by 10–15 % if elution is
performed at 55 °C.

MACHEREY-NAGEL – 06/2021, Rev. 048
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3 Storage conditions and preparation of working
solutions
Attention: Buffers MP1 and MP2 contain chaotropic salt! Wear gloves and goggles!
CAUTION: Buffers MP1 and MP2 contain guanidine hydrochloride which can form highly
reactive compounds when combined with bleach (sodium hypochlorite). DO NOT add bleach
or acidic solutions directly to the sample-preparation waste.
Storage conditions:
• All components of the NucleoMag®96PCR kit should be stored at room temperature
(15–25 °C) and are stable for up to one year.
Before starting any NucleoMag®96 PCR protocol, prepare the following:
• Wash Buffer MP3: Add the indicated volume of ethanol (96–100 %) to Buffer MP3
Concentrate. Mark the label of the bottle to indicate that ethanol was added.
Wash Buffer MP3 is stable at room temperature (15–25 °C) for at least one year.
NucleoMag®96 PCR
REF 1 x 96 preps
744100.1
4 x 96 preps
744100.4
24 x 96 preps
744100.24
Buffer MP3
(Concentrate)
25 mL
Add 100 mL ethanol
100 mL
Add 400 mL ethanol
2 x 200 mL
Add 800 mL ethanol
to each vial

9MACHEREY-NAGEL – 06/2021, Rev. 04
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4 Safety instructions
The following components of the NucleoMag®96 PCR kits contain hazardous contents.
Wear gloves and goggles and follow the safety instructions given in this section.
Component Hazard contents GHS
symbol
Hazard
phrases
Precaution
phrases
Inhalt Gefahrstoff GHS-Symbol H-Sätze P-Sätze
MP1 guanidine hydrochloride
30–50 %
Guanidinhydrochlorid 36–50 %
CAS 50-01-1 WARNING
ACHTUNG
302, 319 264W, 280sh,
301+312, 330
MP2 guanidine hydrochloride
24–36 %
Guanidinhydrochlorid 24–36 %
CAS 50-01-1 WARNING
ACHTUNG
302 264W, 301+312,
330
The symbol shown on labels refers to further safety information in this section.
Das auf Etiketten dargestellte Symbol weist auf weitere Sicherheitsinformationen dieses Kapitels hin.
Hazard phrases
H 302 Harmful if swallowed.
Gesundheitsschädlich bei Verschlucken.
H 319 Causes serious eye irritation.
Verursacht schwere Augenreizung.
Hazard phrases
P 264W Wash with water thoroughly after handling
Nach Gebrauch mit Wasser gründlich waschen.
P 280sh Wear protective gloves / eye protection.
Schutzhandschuhe / Augenschutz tragen.
P 302 IF SWALLOWED: Call a POISON CENTER/doctor if you feel unwell.
BEI VERSCHLUCKEN: Bei Unwohlsein GIFTINFORMATIONSZENTRUM/Arzt anrufen.
P 330 Rinse mouth.
Mund ausspülen.
For further information please see Material Safety Data Sheets (www.mn-net.com).
Weiterführende Informationen finden Sie in den Sicherheitsdatenblättern (www.mn-net.com).

MACHEREY-NAGEL – 06/2021, Rev. 0410
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5 Protocol for clean-up of 50 μL PCR reactions
Protocol-at-a-glance
• For additional equipment and hardware requirements, refer to section 1.2 and 2.3,
respectively.
• For detailed information on each step, see page 12.
Before starting the preparation:
• Check if Buffer MP3 was prepared according to section 3.
1 Transfer PCR reaction
mixture to appropriate
96-well plate
50 μL PCR reaction
For PCR reactions < 50 μL, adjust
the volume to 50 μL
using sterile water
2 Bind DNA to
NucleoMag®P-Beads
12 μL NucleoMag®P-Beads
138 μL MP1
Mix by shaking
for 5 min at RT
(Optional: Mix by pipetting
up and down)
Remove supernatant
after 2 min separation
3 Wash with MP2 Remove Square-well Block
from NucleoMag®SEP
300 μL MP2
Resuspend: Shake 5 min at RT
(Optional: Mix by pipetting
up and down)
Remove supernatant
after 2 min separation

11MACHEREY-NAGEL – 06/2021, Rev. 04
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4 1st wash with MP3 Remove Square-well Block
from NucleoMag®SEP
300 μL MP3
Resuspend: Shake 5 min at RT
(Optional: Mix by pipetting
up and down)
Remove supernatant
after 2 min separation
5 2nd wash with MP3 Remove Square-well Block
from NucleoMag®SEP
300 μL MP3
Resuspend: Shake 5 min at RT
(Optional: Mix by pipetting
up and down)
Remove supernatant
after 2 min separation
6 Dry the beads 10 min at RT
7 Elute DNA Remove Square-well Block
from NucleoMag®SEP
25–100 μL MP4
(Optional: Elute at 55 °C)
Shake 5 min at RT
(Optional: Mix by pipetting
up and down)
Separate 2 min and transfer DNA
into Elution Plate U-bottom

MACHEREY-NAGEL – 06/2021, Rev. 0412
NucleoSpin®96 Soil – vacuum processing
Detailed protocol
This protocol is designed for magnetic separators with static pins (e.g., NucleoMag®SEP)
and suitable plate shakers (see section 2.3). It is recommended using a Square-well Block
for separation (see section 1.2). Alternatively, isolation of DNA can be performed in reaction
tubes with suitable magnetic separators. This protocol is for manual use and serves as a
guideline for adapting the kit to robotic instruments.
Before starting the preparation:
• Check if Buffer MP3 was prepared according to section 3.
1 Transfer PCR reaction mixture
Transfer PCR reaction mixture to an appropriate 96-well plate.
For PCR reaction volumes < 50 μL, adjust the volume to 50 μL using sterile water.
Note: See recommendations for suitable plates (e.g., Square-well Block; not included
in the kit) and compatible magnetic separators section 2.3.
2Bind DNA to NucleoMag®P-Beads
Add 12 μL NucleoMag®P-Beads and 138 μL Binding Buffer MP1 to each well of
the separation plate. Mix by pipetting up and down 6 times and shake for 5 min at
room temperature. Alternatively, when processing the kit without a shaker, pipette
up and down 10 times and incubate for 5 min at room temperature.
Note: NucleoMag®P-Beads and Binding Buffer MP1 may be premixed. For 96
samples, premix 1248 μL of NucleoMag®P-Beads with 14.35 mL of Buffer MP1,
mix by vortexing. Use 150 μL of the suspension per well. Be sure to resuspend the
NucleoMag®P-Beads before removing them from the storage bottle. Vortex storage
bottle briefly until a homogenous suspension has been formed.
Separate the magnetic beads against the side of the wells by placing the 96-well plate
on the NucleoMag®SEP magnetic separator. Wait at least 2 min until all the beads
have been attracted to the magnets. Remove and discard supernatant by pipetting.
Note: Do not disturb the attracted beads while aspirating the supernatant. The
magnetic pellet is not visible in this step. Remove supernatant from the opposite side
of the well.
3 Wash with MP2
Remove the Square-well Block from the NucleoMag®SEP magnetic separator.
Add 300 μL Buffer MP2 to each well and resuspend the beads by shaking until
the beads are resuspended completely (5 min). Alternatively, resuspend beads
completely by repeated pipetting up and down (15 times).
Separate the magnetic beads by placing the Square-well Block on the NucleoMag®
SEP magnetic separator. Wait at least 2 min until all the beads have been attracted
to the magnet. Remove and discard supernatant by pipetting.

13MACHEREY-NAGEL – 06/2021, Rev. 04
NucleoSpin®96 Soil – vacuum processing
4 1st wash with MP3
Remove the Square-well Block from the NucleoMag®SEP magnetic separator.
Add 300 μL Buffer MP3 to each well and resuspend the beads by shaking until
the beads are resuspended completely (5 min). Alternatively, resuspend beads
completely by repeated pipetting up and down (15 times).
Separate the magnetic beads by placing the Square-well Block on the NucleoMag®
SEP magnetic separator. Wait at least 2 min until all the beads have been attracted
to the magnet. Remove and discard supernatant by pipetting.
5 2nd wash with MP3
Remove the Square-well Block from the NucleoMag®SEP magnetic separator.
Add 300 μL Buffer MP3 to each well and resuspend the beads by shaking until
the beads are resuspended completely (5 min). Alternatively, resuspend beads
completely by repeated pipetting up and down (15 times).
Separate the magnetic beads by placing the Square-well Block on the NucleoMag®
SEP magnetic separator. Wait at least 2 min until all the beads have been attracted
to the magnet. Remove and discard supernatant by pipetting.
6Dry the beads
Dry the beads by incubating the Square-well Block 10 min at room temperature
with the particles held against the magnet in order to allow the remaining traces of
alcohol to evaporate.
Note: Allow the pellet to dry sufficiently so that there is no visible droplets of buffer
in the bottom of the tube. Allowing the pellet to dry completely, indicated by visible
cracking. Do not overdry beads (e.g., by prolonged drying at 55 °C). This will reduce
yield.
7Elute DNA
Remove the Square-well Block from the NucleoMag®SEP magnetic separator.
Add desired volume of Elution Buffer MP4 (25–100 μL) to each well and resuspend
the by shaking 5–10 min at 56 °C. Alternatively, resuspend beads completely by
repeated pipetting up and down and incubate for 5–10 min at room temperature.
Separate the magnetic beads by placing the Square-well Block on the
NucleoMag®SEP magnetic separator. Wait at least 2 min until all the beads have
been attracted to the magnets. Transfer the supernatant containing the purified PCR
products to the Elution Plate U-bottom.
Note:Yield can be increased by 15–20 % by using pre-warmed elution buffer (55 °C)
or by incubating the bead / elution buffer suspension at 55 °C for 5 min.

MACHEREY-NAGEL – 06/2021, Rev. 0414
PCR clean-up
6 Appendix
6.1 Troubleshooting
Problem Possible cause and suggestions
Poor DNA yield
Wash Buffer MP3 did not contain ethanol
• Addition of the the indicated volume of 96–100 % ethanol to
Buffer MP3 Concentrate is required before use.
Elution buffer volume insufficient
• Bead pellet must be covered completely with elution buffer.
Insufficient performance of elution buffer during elution step
• Remove residual wash buffers during the separation steps
completely. Remaining buffers decrease efficiency of following
wash steps and elution step.
Beads overdried
• Do not dry beads longer than 20 min at room temperature.
Overdrying of beads may result in lower elution efficiencies.
Suboptimal
performance
of DNA in
downstream
applications
Carry-over of ethanol from Wash Buffer MP3
• Be sure to remove all of the ethanolic Wash Buffer MP3 after the
final wash step. Dry beads 10–15 min at room temperature.
Elution of DNA with TE buffer
• Use supplied elution buffer or sterile water. Do not use TE buffer.
EDTA may inhibit sequencing reactions. Repurify or precipitate
DNA by ethanol and elute / redissolve in Elution Buffer MP 4
buffer or water.
Eluted DNA contains residual primers / primer dimers
• Minimize amount of primers in PCR reaction mixture. Do not use
higher volumes of binding buffer than specified.

15MACHEREY-NAGEL – 06/2021, Rev. 04
PCR clean-up
Problem Possible cause and suggestions
Carry-over of
beads
Time for magnetic separation too short
• Increase separation time to allow the beads to be attracted to the
magnetic pins completely.
Aspiration speed too high (elution step)
• High aspiration speeds during the elution step may cause bead
carry-over. Reduce aspiration speed for elution step.
6.2 Ordering information
Product REF Pack of
NucleoMag®96 PCR 744100.1
744100.4
744100.24
1 x 96 preps
4 x 96 preps
24 x 96 preps
NucleoMag®SEP 744900 1
Square-well Blocks 740481
740481.24
4
24
Elution Plate U-bottom 740486.24 24
Self-adhering PE Foil 740676 50 sheets
Visit www.mn-net.com for more detailed product information.

MACHEREY-NAGEL – 06/2021, Rev. 0416
PCR clean-up
6.3 Product use restriction / warranty
NucleoMag®96 PCR kit components are intended, developed, designed, and sold FOR
RESEARCH PURPOSES ONLY, except, however, any other function of the product being
expressly described in original MACHEREY-NAGEL product leaflets.
MACHEREY-NAGEL products are intended for GENERAL LABORATORY USE
ONLY! MACHEREY-NAGEL products are suited for QUALIFIED PERSONNEL ONLY!
MACHEREY-NAGEL products shall in any event only be used wearing adequate
PROTECTIVE CLOTHING. For detailed information please refer to the respective Material
Safety Data Sheet of the product! MACHEREY-NAGEL products shall exclusively be used
in an ADEQUATE TEST ENVIRONMENT. MACHEREY-NAGEL does not assume any
responsibility for damages due to improper application of our products in other fields of
application. Application on the human body is STRICTLY FORBIDDEN. The respective user
is liable for any and all damages resulting from such application.
DNA/RNA/PROTEIN purification products of MACHEREY-NAGEL are suitable for IN VITRO-
USES ONLY!
ONLY MACHEREY-NAGEL products specially labeled as IVD are also suitable for IN VITRO-
diagnostic use. Please pay attention to the package of the product. IN VITRO-diagnostic
products are expressly marked as IVD on the packaging.
IF THERE IS NO IVD SIGN, THE PRODUCT SHALL NOT BE SUITABLE FOR IN VITRO-
DIAGNOSTIC USE!
ALL OTHER PRODUCTS NOT LABELED AS IVD ARE NOT SUITED FOR ANY CLINICAL
USE (INCLUDING, BUT NOT LIMITED TO DIAGNOSTIC, THERAPEUTIC AND/OR
PROGNOSTIC USE).
No claim or representations is intended for its use to identify any specific organism or for
clinical use (included, but not limited to diagnostic, prognostic, therapeutic, or blood banking).
It is rather in the responsibility of the user or - in any case of resale of the products - in the
responsibility of the reseller to inspect and assure the use of the DNA/RNA/protein purification
products of MACHEREY-NAGEL for a well-defined and specific application.
MACHEREY-NAGEL shall only be responsible for the product specifications and the
performance range of MN products according to the specifications of in-house quality control,
product documentation and marketing material.
This MACHEREY-NAGEL product is shipped with documentation stating specifications and
other technical information. MACHEREY-NAGEL warrants to meet the stated specifications.
MACHEREY-NAGEL’s sole obligation and the customer’s sole remedy is limited to replacement
of products free of charge in the event products fail to perform as warranted. Supplementary
reference is made to the general business terms and conditions of MACHEREY-NAGEL,
which are printed on the price list. Please contact us if you wish to get an extra copy.
There is no warranty for and MACHEREY-NAGEL is not liable for damages or defects arising
in shipping and handling (transport insurance for customers excluded), or out of accident or
improper or abnormal use of this product; defects in products or components not manufactured
by MACHEREY-NAGEL, or damages resulting from such non-MACHEREY-NAGEL
components or products.
MACHEREY-NAGEL makes no other warranty of any kind whatsoever, and SPECIFICALLY
DISCLAIMS AND EXCLUDES ALL OTHER WARRANTIES OF ANY KIND OR NATURE
WHATSOEVER, DIRECTLY OR INDIRECTLY, EXPRESS OR IMPLIED, INCLUDING,

17MACHEREY-NAGEL – 06/2021, Rev. 04
PCR clean-up
WITHOUT LIMITATION, AS TO THE SUITABILITY, REPRODUCTIVITY, DURABILITY,
FITNESS FOR A PARTICULAR PURPOSE OR USE, MERCHANTABILITY, CONDITION,
OR ANY OTHER MATTER WITH RESPECT TO MACHEREY-NAGEL PRODUCTS.
In no event shall MACHEREY-NAGEL be liable for claims for any other damages, whether
direct, indirect, incidental, compensatory, foreseeable, consequential, or special (including
but not limited to loss of use, revenue or profit), whether based upon warranty, contract,
tort (including negligence) or strict liability arising in connection with the sale or the failure
of MACHEREY-NAGEL products to perform in accordance with the stated specifications.
This warranty is exclusive and MACHEREY-NAGEL makes no other warranty expressed or
implied.
The warranty provided herein and the data, specifications and descriptions of this
MACHEREY-NAGEL product appearing in MACHEREY-NAGEL published catalogues and
product literature are MACHEREY-NAGEL’s sole representations concerning the product and
warranty. No other statements or representations, written or oral, by MACHEREY-NAGEL’s
employees, agent or representatives, except written statements signed by a duly authorized
officer of MACHEREY-NAGEL are authorized; they should not be relied upon by the customer
and are not a part of the contract of sale or of this warranty.
Product claims are subject to change. Therefore please contact our Technical Service
Team for the most up-to-date information on MACHEREY-NAGEL products. You
may also contact your local distributor for general scientific information. Applications
mentioned in MACHEREY-NAGEL literature are provided for informational purposes
only. MACHEREY-NAGEL does not warrant that all applications have been tested in
MACHEREY-NAGEL laboratories using MACHEREY-NAGEL products. MACHEREY-NAGEL
does not warrant the correctness of any of those applications.
Last updated: 07 / 2010, Rev. 03
Please contact:
MACHEREY-NAGEL GmbH & Co. KG
Tel.: [phone]
[email protected]
Trademarks:
KingFisher is a registered trademark of Thermo Fisher Scientific
NucleoMag is a registered trademark of MACHEREY-NAGEL GmbH & Co KG
Te-MagS is a trademark of Tecan Group Ltd., Switzerland

Plasmid DNA
Clean up
RNA
DNA
Viral RNA and DNA
Protein
High throughput
Accessories
Auxiliary tools

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www.mn-net.com
DE Tel.: [phone] [email protected]
CH Tel.: [phone] [email protected]
FR Tel.: [phone] [email protected]
US Tel.: [phone] [email protected]
MACHEREY-NAGEL GmbH & Co. KG
Valencienner Str. 11
52355 Düren · Germany
A027276/0610.1