Hanna Instruments HI931 User manual

MANUALS
AUTOMATIC
POTENTIOMETRIC TITRATOR
HI931

TABLE OF CONTENTS
HI931 AUTOMATIC POTENTIOMETRIC TITRATOR
Congratulations on choosing your new Hanna titrator. It is a powerful and versatile instrument capable of accurate and fast
analysis of a wide range of samples. In this manual you’ll find:
QUICK START GUIDE
This guide will help you quickly setup, operate, and introduce you to your new titrator. It covers basic connections, user interface,
how to perform calibrations, and how to run a titration.
INSTRUCTION MANUAL
The manual provides a comprehensive description of the operating principles user interface, general options, methods, titration/
direct reading mode, pH, mV and ISE mode, maintenance, etc.
APPLICATIONS BROCHURE
This brochure contains complete instructions for commonly-used analyses. Additional methods and method packs are available;
contact your local Hanna office for more details.
TITRATION THEORY
This guide outlines the principles of operation of the titrator. It covers the chemistry of titrations, titration types, and result
calculations.
If you need additional technical information, do not hesitate to e-mail us at [email protected] or view our worldwide contact
list for a Hanna Instruments representative near you at www.hannainst.com.

QUICK START GUIDE
AUTOMATIC
POTENTIOMETRIC TITRATOR
HI931

Congratulations on choosing a Hanna Instruments product.
Please read this Quick Start Guide carefully before using the instrument. This guide will provide you with the necessary
information for the correct use of the instrument.
The purpose of this guide is to present a quick overview of setting up and using the instrument.
For detailed information illustrating the extensive capabilities of your titrator, please refer to the Instruction Manual.
Dear
Customer,
© 2018 Hanna Instruments
All rights are reserved. Reproduction in whole or in part is prohibited without the written consent of the copyright owner,
Hanna Instruments Inc., Woonsocket, Rhode Island, 02895, USA.

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INTRODUCTION.................................................................................................................................................................
SAFETY MEASURES ...........................................................................................................................................................
TITRATOR CONNECTIONS .................................................................................................................................................
USER INTERFACE...............................................................................................................................................................
HOW TO SELECT YOUR LANGUAGE..................................................................................................................................
HOW TO USE THE CONTEXTUAL HELP.............................................................................................................................
METHODS..........................................................................................................................................................................
HOW TO CALIBRATE A pH ELECTRODE.............................................................................................................................
HOW TO PERFORM A TITRATION......................................................................................................................................
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INTRODUCTION
The HI931 automatic titrator is designed to perform a wide variety of potentiometric titrations with high accuracy, flexibility and
reproducibility, allowing the user to obtain both accurate results and high-speed analysis.
The titrator can perform fixed endpoint or equivalence point titrations and direct measurements by measuring the pH/mV and
temperature of the sample.
Reports and methods can be transferred to a PC via a USB interface, saved to a USB storage device or printed directly from the
titrator. An external keyboard can also be attached for added convenience.
How can I find certain information?
- The Quick Start Guide will help the user learn how to operate the titrator within a short period of time.
- The Instruction Manual provides a complete description of the operating principles (user interface, general options, methods,
titration, pH, mV and ISE mode, maintenance, etc.).
- The Titration Theory outlines the basic concepts of titration.
- The contextual Help screens contain detailed explanations of every screen.
SAFETY MEASURES
The following safety measures must be followed:
1. Never connect or disconnect the pump assembly or other peripheral with the titrator turned on.
2. Verify that the burette and the attached tubing are assembled correctly.
3. Always check that the titrant bottle and the titration beaker are placed on a flat, stable surface.
4. Always wipe up spills and splashes immediately.
5. Avoid the following environmental working conditions:
• Severe vibrations
• Direct sunlight
• Atmospheric relative humidity above 95% non-condensing
• Environment temperatures below 10 ° C and above 40 ° C
• Explosion hazards
6. Have the titrator serviced by qualified service personnel only.

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TITRATOR CONNECTIONS
FRONT VIEW
REAR VIEW

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USER INTERFACE
Keypad
The titrator’s keypad has 27 keys grouped in five categories, as follows:
Display
The user interface contains several screens. In each screen, many information fields are present at the same time. The information is
displayed in an easy-to-read manner.
Virtual option keys describe the function performed when the corresponding option key is pressed.

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HOW TO SELECT YOUR LANGUAGE
To change the language, press from the main screen. Highlight the Language option. Using the and keys, select
the language and press .
Restart the titrator in order to apply the new language setting.
HOW TO USE THE CONTEXTUAL HELP
Information about the titrator can be easily accessed by pressing . The contextual help can be accessed at any time and it
provides useful information about the current screen.
METHODS
The HI931 titrator can store up to 100 methods (standard and user).
Standard Methods
Each titrator is supplied with a package of standard methods. Standard method packs are developed at Hanna Instruments to meet
analysis requirements of specific industries (e.g., water treatment, wine, dairy, etc.).
User-Defined Methods
User defined methods allow the user to create and save their own methods. Each new method is based on an existing method which
is altered to suit a specific application.
HOW TO CALIBRATE A pH ELECTRODE
To enter pH calibration mode, press , then , then .
PREPARATION
Pour small quantities pH 4.01, pH 7.01 and pH 10.01 buffer solutions into clean beakers. If possible, use plastic beakers to
minimize any EMC interferences.
For accurate calibration and to minimize cross-contamination, use two beakers for each buffer solution: one for rinsing the electrode
and one for calibration.
CALIBRATION PROCEDURE
• If the instrument has been previously calibrated and calibration was not cleared, the old calibration can be cleared by pressing .
Note: It is very important to clear calibration history when a new electrode is used. Most errors and warning messages that appear
during calibration depend on calibration history.

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• Use the or to select pH 4.01 buffer solution.
• Use the second beaker of pH 4.01 buffer solution to rinse the pH electrode, temperature probe and propeller stirrer.
• Immerse the pH electrode, temperature probe and propeller stirrer in the pH 4.01 buffer solution. The pH electrode’s bulb must
be completely immersed in the buffer solution and the reference junction needs to be 5-6 mm below the surface. Add additional
buffer if necessary.
• Press to turn on the propeller stirrer.
• Once the reading has stabilized, press to update the calibration.
• Repeat this procedure for pH 7.01 and 10.01 buffer solutions.
• Press to accept and exit pH calibration mode.
HOW TO PERFORM A TITRATION
Required Solutions
• Titrant - 500 mL of 0.1 M (mol/L) Sodium Hydroxide (NaOH) in a titrant bottle.
• Sample - 0.1 mol/L Hydrochloric Acid (HCl).
• Distilled or deionized water.
Note: Analytical grade reagents and water should be used for accurate results.
Priming the Burette
• Insert the aspiration tube in the titrant bottle and the dispensing tube in a waste beaker.
• From the main screen press .
•Highlight the Prime Burette option and then press .
•Enter the number of burette rinses. At least 3 rinses are recommended.
•Press to start.
•The message “Executing...” will be displayed.
Note: Make sure you have continuous liquid flow inside the burette. For accurate results, the aspiration tube, the dispensing tube
and the syringe must be free of air bubbles.
Method Selection
For this analysis, we will use the HI1009 Neutralization w/ NaOH.
To select this method:
• Press . Use the and keys to highlight HI1009 Neutralization w/ NaOH.
• Press .
Setting Method Parameters
To display the method parameters, press .
The View/Modify Method screen will be displayed.
Only certain parameters can be changed.
For this titration, the NaOH titrant concentration and the size of the HCl sample need to be entered.
To accomplish this:
• Highlight Titrant Conc. option, then press .
The Titrant Concentration screen will be displayed.
• Enter the correct value, then press .
• Highlight Analyte Size option, then press .
• Enter the volume of the sample (e.g.: 5 mL), then press .
• Press , highlight Save Method option and then press .

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Setup Titration Report
Users can select the information that is stored for each titration.
To setup the titration report, follow the procedure below:
• From the main screen, press . The Data Parameters screen will be displayed.
• Highlight Setup Titration Report and press .
• Mark the fields to be included in the titration report with the “*” symbol. Use the and keys to highlight a field and
/ to toggle the field.
• Press to save the customized report .
Preparing the Sample
• Add 50 to 65 mL of distilled/deionized water to the titration beaker.
• Use a pipette or burette to add 5.0 mL of the sample (0.1M Hydrochloric Acid (HCl)) into the same beaker.
• Slide the stirrer assembly up.
• Place the beaker under the stirrer assembly.
• Lower the stirrer assembly until the electrodes are submersed and the stirrer is close to the bottom of the beaker.
• Adjust the level of the sample solution with distilled/deionized water so that the pH electrode bulb is completely immersed in
the sample solution and the reference junction of the electrode is 5-6 mm below the surface.
Performing a Titration
• From the main screen, press . You will be prompted to enter the analyte size. Enter 5 mL and press . The titrator will
start the analysis.
• At the end of the titration, the message “Titration Completed” will appear on the display with the final concentration of the
analyte in the sample and the equivalence endpoint volume.
Understanding the Displayed Information
During a titration the following screen is displayed:

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Viewing Graph During Titration
After a few doses are dispensed, will become active. Press to display the real-time titration graph.
The curves displayed are plots of the pH and the 1st derivative versus Titrant Volume (for details, see the Instruction Manual).
The two graphs are scaled to fit in the same screen window. Press to change the y-axis scale to either the pH values or the
1st derivative values.
Titration Termination
The titration is normally terminated when the first equivalence endpoint is detected according to the selected algorithm. To ensure the
correct detection and interpolation of the equivalence endpoint, the titrator will dispense a few additional doses after the endpoint
was reached.
The titration result can be displayed either in the main screen or in the Graph of Titration Data screen:

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When the titration has ended, the titrator will display the equivalence endpoint volume and the final concentration of the analyte
together with the "Titration Completed" message.
To view the titration graph and/or results, press .
When the titration ends, an “x” will mark the endpoint on the pH versus titrant volume curve in the Graph of Titration Data
screen. The value of the endpoint volume is also displayed next to the endpoint.
Results
The results obtained from a titration are stored in a report file that can be viewed, transferred to a USB Storage Device or PC and
printed.
Viewing the last titration data
• From the main screen, press . The Data Parameters screen will be displayed.
• From the Data Parameters screen highlight the Review Last Analysis Report option and press . The Review Result
screen will be displayed.
• Use the and keys to display information related to the last titration performed.
See Titration Report on next page.
Printing the titration report
Connect a DOS / Windows-compatible parallel printer directly to the DB 25-pin connector located on the back of the titrator.
Note: When connecting the printer, please turn off the titrator and the printer.
Printing out the report:
• From the Review Report screen, press .
• During the information transfer to the printer, the message “Printing” will be displayed on the screen.
• Press to return to the Data Parameters screen.
• Press again to return to the main screen.
Saving data to USB Storage Device
This feature allows saving the results of titrations or pH / mV / ISE logging sessions on a USB storage device.
• From the main screen, press , the General Options screen will be displayed.
• Highlight the Save Files to USB Storage Device option using the and keys.
• Insert the USB storage device into the USB socket.
• Press , the List of Files on Titrator screen will be displayed.

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HI931 - Titration Report
Method Name: Neutralization w/ NaOH
Time & Date: 15:01 Jun 13, 2018
Report ID: Ti_00011
Calibration Data
 Buer Potential EciencyTemp.
Time and Date
 4.010pH169.3mV98.8%24.0°CA
11:44 Jun 13, 2018
 7.010pH-5.8mV98.7%23.9°CA
11:42 Jun 13, 2018
 10.010pH-180.7mV98.7%24.0°CA
  11:46Jun13,2018
 GLP&MeterInformation
Sample Name:
 CompanyName:
Operator Name:
 ElectrodeName:
Field 1:
Field 2:
Field 3:
 TitratorSoftwareVersion:v1.00
 BaseBoardSoftwareVersion:v1.00
 Pump1SoftwareVersion:v1.00
 Stirrer1SoftwareVersion:v1.00
Titrator Serial Number: TT180525011
 AnalogBoard1SerialNumber:AB180525005
 Pump1SerialNumber:DP180525004
Stirrer 1 Serial Number: OS180524001
 Analog1CalibrationDate:May25,2018
 MethodParameters
Name: Neutralization w/ NaOH
 MethodRevision:3.0
 AnalysisType:StandardTitration
 StirrerConguration:
Stirrer: Stirrer 1
 StirringSpeed:1400RPM
• Use the and keys to select the report files.
• Press to transfer all available reports to the USB storage device, or highlight the name of the report file to be
transferred and press .
• Transferring a report file will automatically transfer the corresponding log file and titration graph (*.BMP file if applicable).
• Press to return to the General Options screen.
• Press again to return to the main screen.
Titration report
While scrolling with the and keys, the fields below can be seen on the titrator display or printed. The same
information is available on the saved report file (Ti_00007.rpt in this example).

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 PumpConguration:
 Titrantpump:Pump1
 DosingType:Dynamic
 MinVol:0.050mL
 MaxVol:0.500mL
 deltaE:20.000mV
 EndPointMode:pH1EQpoint,1stDer
 RecognitionOptions
 Threshold:50mV/mL
 Range:NO
 FilteredDerivatives:NO
 Pre-TitrationVolume:0.000mL
 Pre-TitrationStirTime:0sec
 MeasurementMode:SignalStability
 deltaE:1.0mV
 deltat:2sec
 Minwait:2sec
 Maxwait:15sec
 ElectrodeType:pH
Blank Option: No Blank
 Calculations:SampleCalc.byVolume
 DilutionOption:Disabled
 TitrantName:0.1NHaOH
 TitrantConc.:0.1000N(eq/L)
 AnalyteSize:10.0000mL
 AnalyteEntry:Fixed
 MaximumTitrantVolume:20.000mL
 PotentialRange:-2000.0to2000.0mV
 Volume/FlowRate:25mL/50.0mL/min
 SignalAveraging:1Reading
 SignicantFigures:XXXXX
 N(eq/L)-->meq/L
 Veq1000meq
-*--*-------
 Leq
-------------
mL L
--*------
1000mL
 V=volumedispensedinliters.
 0.100eq/L->titrantconc.
 10.000mL->samplevolume
 NrVolume[mL]mVpHGraphicTemp.[°C]Time
 00.000274.42.2190.024.9A00:00:00
 10.050274.42.2201.025.0A00:00:07
 20.100274.42.2200.025.0A00:00:10
 30.200274.32.222-0.825.0A00:00:12
 40.400274.02.227-1.625.0A00:00:15
 50.800273.22.241-2.025.0A00:00:18
 61.300271.52.271-3.425.0A00:00:24
 71.800269.52.304-3.925.1A00:00:30
 82.300267.22.344-4.725.1A00:00:37
 92.800264.42.393-5.725.1A00:00:43
 103.300260.82.455-7.225.1A00:00:50
 113.800256.12.535-9.325.1A00:00:58
 124.300250.32.635-11.725.1A00:01:05
 134.800241.92.779-16.825.1A00:01:14
 145.300228.33.011-27.225.1A00:01:23

14
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 155.800193.03.614-70.525.1A00:01:31
 166.07721.06.556-620.025.1A00:01:48
 176.128-38.27.568-1183.225.1A00:02:03
 186.177-123.69.031-1708.025.1A00:02:19
 196.227-157.79.616-682.825.1A00:02:28
 206.278-174.59.903-335.825.1A00:02:35
 216.339-187.810.130-215.925.1A00:02:42
 TitrationResults
Method Name: Neutralization w/ NaOH
Time & Date: 15:01 Jun 13, 2018
 AnalyteSize:10.0000mL
 EndPointVolume:6.144mL
 pHEquivalencePoint:8.063
 Result:61.444meq/L
 Initial&FinalpH:2.219to10.130
 TitrationDuration:2:42[mm:ss]
Titration went to Completion
 AnalystSignature:____________________
QS931 10/18

INSTRUCTION MANUAL
AUTOMATIC
POTENTIOMETRIC TITRATOR
HI931