Promega Maxwell 16 MDx User manual

Revised 4/15
TM320
OPERATING MANUAL
Maxwell®16
MDx Instrument
Instrucons for Use of Product
AS3000
2800
Woods Hollo
w Rd.
Madison, WI USA

Promega Corporaon · 2800 Woods Hollow Road · Madison, WI 53711-5399 USA · Toll Free in USA 800-356-9526 · 608-274-4330 · Fax [phone]
www.promega.com TM320 · Revised 4/15
All technical literature is available at: www.promega.com/protocols/
Visit the web site to verify that you are using the most current version of this Technical Manual.
E-mail Promega Technical Services if you have questions on use of this system: [email protected]
Maxwell®16 MDx
Instrument Operating Manual
1. Introduction............................................................................................................................................3
1.A. Maxwell®16 MDx Purification Procedure.........................................................................................3
1.B. Maxwell®16 MDx Instrument Specifications .................................................................................... 4
1.C. Product Components and Symbol Key ..............................................................................................5
1.D. Inspection ....................................................................................................................................... 6
1.E. Precautions .....................................................................................................................................7
1.F. Environmental Requirements (operating, shipping and storage conditions)........................................9
2. Hardware Overview .............................................................................................................................. 10
3. Hardware Mode .................................................................................................................................... 13
4. Unpacking and Setting Up the Maxwell®16 MDx Instrument.............................................................. 14
4.A. Unpacking..................................................................................................................................... 14
4.B. Removing the Magnetic Rod Assembly/Plunger Bar Platform Shipping Anchors .............................. 14
4.C. UV Bulb Installation ...................................................................................................................... 15
4.D. Maxwell®Sample Track Software................................................................................................... 16
4.E. Operational Setup.......................................................................................................................... 16
5. Operating the Maxwell®16 MDx Instrument ....................................................................................... 23
5.A. Preprogrammed Methods .............................................................................................................. 23
5.B. Setting Up Samples and Collecting Input Data ................................................................................ 25
5.C. Performing a Sample Purification Run............................................................................................ 29
5.D. Post-Run UV Light Treatment........................................................................................................ 30
6. Run Reports .......................................................................................................................................... 31
6.A. Data File Transfer at the End of a Run ............................................................................................ 31
6.B. Data File Transfer at a Later Time .................................................................................................. 32
6.C. Data File Management................................................................................................................... 32
7. Changing Configuration Parameters ..................................................................................................... 32
7.A. Changing the Hardware ................................................................................................................. 33
7.B. Changing the Instrument Firmware Configuration .......................................................................... 36

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8. Cleaning and Maintenance ................................................................................................................... 37
8.A. General Care ................................................................................................................................. 37
8.B. Cleaning the Hardware ..................................................................................................................38
8.C. Dealing with Spills......................................................................................................................... 38
8.D. Decontamination Using the UV Lamp............................................................................................. 38
8.E. Replacing the UV Bulb................................................................................................................... 39
8.F. Periodic Maintenance .................................................................................................................... 39
9. Troubleshooting.................................................................................................................................... 40
10. Appendix............................................................................................................................................... 45
10.A. User-Defined Methods ................................................................................................................... 45
10.B Homogenization Method ............................................................................................................... 52
10.C. Updating Firmware........................................................................................................................ 53
10.D. Service .......................................................................................................................................... 53
10.E. Instrument Return......................................................................................................................... 55
10.F. Instrument Disposal ...................................................................................................................... 56
11. Warranties, Service Agreements and Related Products ........................................................................ 57
11.A. Warranty Information.................................................................................................................... 57
11.B. Warranty and Service Agreement Options....................................................................................... 58
11.C. Related Products ........................................................................................................................... 60
12. Certificate of Decontamination.................................................................................................... 61
13. Summary of Changes .................................................................................................................. 62

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1. Introduction
1.A. Maxwell®16 MDx Purification Procedure
The Maxwell®16 MDx Instrument(a) provides automated nucleic acid purification methods for multiple analytes.
The methods use sample lysis and binding to paramagnetic particles as the primary separation principle. It has
two modes for different elution volumes. The Standard Elution Volume (SEV) mode allows elution in a volume of
up to 400µl. The Low Elution Volume (LEV) mode allows elution in as little as 25µl. Up to 16 samples can be
prepared in a single run.
The automated steps performed by the Maxwell®16 MDx Instrument include:
• Sample lysis in the presence of a chaotropic agent, detergent and/or alcohol
• Binding of nucleic acids or proteins to paramagnetic particles
• Washing of the target molecules bound to the particles away from other cellular components
• Elution of the product
The instrument contains a touch screen for navigating, programming and running the instrument. It has a UV
lamp to aid with decontamination. The Maxwell®16 MDx Instrument, in association with the Maxwell®Sample
Track Software, has the ability to record and provide run data. It has a USB port that can be used to attach a bar
code reader, allowing sample and reagent information to be entered using bar codes. The Maxwell®16 MDx
Instrument can report the data gathered for each run, and the report can be sent to a serial printer or to a
computer.
The user selects the protocol to be run, collects the selected bar code information (optional) and places the
samples into the reagent cartridges. The cartridges are placed into the machine and the door shut to start the run,
which automatically performs the protocol.
The temperature of the samples is regulated by a heating system that is controlled by the protocol.
Maxwell®Sample Track is a communications program that allows downloading and printing of the run data
(see Technical Manual #TM314).

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Maxwell®16 MDx Instrument Features
• Easy-to-use and easy-to-maintain system operation that standardizes nucleic acid or protein sample
preparation workflow
• Comprehensive technical support
• System controlled via multi-language LCD readout
• UV lamp to aid in decontamination of instrument
• Reporting functionality
• Maxwell®Sample Track Software and bar code reader included
• Preprogrammed methods for DNA, RNA and protein purification
• Ability to create user-defined protocols
1.B. Maxwell®16 MDx Instrument Specifications
Processing Time: Depending upon sample type and method used, 20–50 minutes
Number of Samples: Up to 16
Weight: 42.7lb (19.4kg)
Dimensions (W ×D ×H): 12.8 ×17.3 ×12.9 inches (325.5 ×438.2 ×326.5mm)
Power Requirements: 100–240VAC, 50–60Hz, 2.1A
Fuse: 3A time-lag fuse
UV Bulb: Average lifetime approx. 3000 hours, length 134.5mm,
diameter 15.5mm, 4.5W, 0.17A current, 29V, Spectral Peak 253.7,
UV output 0.8W

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1.C. Product Components and Symbol Key
PRODUCT CAT.#
Maxwell® 16 MDx Instrument AS3000
For Research Use Only. Not for Use in Diagnostic Procedures. Includes:
• 1 Maxwell®16 MDx Instrument
• 1 Power Cable
• 1 UV Bulb
• 1 SD Card
• 1 SD Card reader package (contains SD card reader and cable)
• 1 CD containing the technical manual
• 1 Quick Start Guide
• 1 Bar Code Reader (provided in a separate box)
• 1 CD containing Sample Track Software and manual
• 1 RS-232 Cable for communication with a computer or printer
• 1 RS-232/USB Adaptor for data export to computer
• 1 SEV Cartridge Rack
• 1 SEV Magnetic Elution Rack
• 1 SEV Magnetic Rod Assembly
• 1 LEV Cartridge Rack
• 1 LEV Magnetic Rod and Plunger Bar Adapter*
*The Maxwell®16 MDx Instrument includes both the SEV and LEV hardware. The instrument will be
provided configured in the LEV mode, with the LEV Magnetic Rod and Plunger Bar Adapter installed.
Symbol Key
Symbol Explanation Symbol Explanation
Important Catalog number
2800
Woods Hollo
w Rd.
Madison, WI USA
Manufacturer Serial number
SN
!

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1.D. Inspection
Upon receiving your Maxwell®16 MDx Instrument, please inspect the package carefully to make sure all accesso-
ries are present. Standard accessories are shown in Figure 1.
8090TA
11470TA
11546TA
Figure 1. Maxwell®16 MDx Instrument accessories. Panel A. Components shown include: Bar Code
Reader Cable, Bar Code Reader, Power Cord, UV Bulb, SD Card Reader and Cable, SD Card, RS-232/USB
Adaptor Cable, RS-232 Cable. Not pictured: Quick Start Guide, CD containing Technical Manual and CD
containing Sample Track Software. Bar Code Reader and Bar Code Reader cable are provided in a separate box.
Panel B. LEV Cartridge Rack, LEV High-Strength Magnetic Rod, Plunger Bar Adapter. Panel C. SEV Cartridge
Rack, SEV Elution Rack, SEV Magnetic Rod.
B.
A.
C.

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1.E. Precautions
IMPORTANT SAFETY INSTRUCTIONS. SAVE THESE INSTRUCTIONS.
Safety Symbols and Markings
Danger. Hazardous voltage. Risk of electrical shock.
Warning. Risk of personal injury to the operator or a safety hazard to the instrument or
surrounding area.
Warning. Pinch point hazard.
Warning. Hot surface. Burn hazard.
Warning. Lifting hazard.
Warning. Biohazard.
Warning. UV light hazard. Do not look directly at the UV light.
Warning. Wear gloves when handling.
Warning. It is important to understand and follow all laws regarding the safe and proper
disposal of electrical instrumentation. Please contact your local Promega Representative for
disposal of the instrument. Please follow your institutional requirements for disposal of the
accessories.
5294MA.eps
5295MA.eps
6422MA
8252MA
6687TA.eps

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1.E. Precautions (continued)
• Changes or modifications to this unit not expressly approved by the party responsible for compliance
could void the user’s authority to operate the equipment.
• This equipment has been designed and tested to CISPR 11 Class A. In a domestic environment it may
cause radio interference, in which case you may need to take measures to mitigate the interference.
• Do not use this device in proximity to sources of strong electromagnetic radiation (e.g., unshielded
intentional RF sources), as these may interfere with the proper operation.
• Do not use this instrument for anything other than its intended use.
• Always disconnect the power before cleaning or performing routine maintenance.
• Do not disassemble unit.
• Do not override the door sensor. Moving parts or potential exposure to UV light from the bulb may
cause personal injury.
• Ensure cartridges, elution tubes and plungers have been securely inserted in their correct positions and
orientation. Failure to do so may result in damage to the instrument.
• After each run, verify that the plungers have been completely removed from the magnet rods before
pressing Run/Stop to extend the platform.
• Use only Promega Maxwell®16 cartridges and plungers designed for use with the appropriate hardware
configuration of the instrument.
• Do not reuse cartridges, plungers or elution tubes
• Wear gloves when handling the bulb, for example during insertion and removal of the bulb. Do not
operate the UV lamp if the instrument door is open.
• The UV bulb contains mercury and must be disposed of properly. To dispose of a bulb, please follow
your institutional requirements for cleanup and disposal of mercury.
• Use only Promega supplied UV bulbs (Cat.# SP1080).
• If the equipment is used in a manner other than that specified by Promega, the protection provided by
the equipment may be impaired.
• Keep hands clear of instrument platform as it moves in and out of the instrument.
• During elution, the heated elution block at the front of the platform becomes very hot. Do not touch.
• To avoid muscle strain or back injury, use lifting aids and proper lifting techniques when removing or
replacing the instrument. The Maxwell®16 MDx Instrument weighs 42.7lb (19.4kg) and should be
handled by two people.
• Equipment can be hazardous due to the use of chemical and biohazardous substances

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1.F. Environmental Requirements (operating, shipping and storage conditions)
Power Requirements: 100–240VAC, 50–60Hz, 2.1A
Temperature: 5–40°C
Humidity: Up to 80% relative humidity (for operation, shipping and storage)
The Maxwell®16 MDx Instrument is intended for indoor use only. Wipe up spills immediately. Install the
instrument on a clean, level surface. To avoid shortening the expected lifespan of the instrument, install in a
location that meets the following criteria:
• Locate on a sturdy, level surface.
• Avoid dusty areas.
• Choose a location that has good air circulation and is not exposed to direct sunlight.
• Avoid noisy electrical power sources (e.g., power generators).
• Do not install in a location where there is large temperature variability or high humidity.
• Position the instrument so that it is easy to unplug from the power source.
• Do not place next to heat sources.
• Do not use near flammable gases or liquids.
• Do not place near other electrically sensitive instruments.

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2. Hardware Overview
Figures 2 and 3 show the front of the Maxwell®16 MDx Instrument.
8084TA
Figure 2. Front view of the Maxwell®16 MDx Instrument.
8085TA
Figure 3. Touch Screen and Keypad. The touch screen and the keypad for use in navigating through the
various screen displays for the Maxwell®16 MDx Instrument. The same button selections are available on the
keypad and the screen. Both sets of buttons can be used to navigate within and between screens. The Back and
Run/Stop buttons allow navigation between screens. The Up and Down buttons are for navigation within a screen.

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11469TA
On/Off
Switch
Power
Connection
Label
Figure 4. Power On/Offswitch on the rear of the Maxwell®16 MDx Instrument. The rear of the
instrument showing the On/Offswitch and where to connect the power cable. A 3 amp time-lag fuse is located
next to the power switch.
8082T
A
On/off switch
Power cord
receptacle
RS-232 port
USB port
SD card slot
Figure 5. Communications ports on the side of the instrument. This side view shows the communication
ports for the instrument. The USB port is used to attach the bar code reader.

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2. Hardware Overview (continued)
The RS-232 serial port can be used to connect to a computer
or a serial printer using the supplied RS-232 cable. If your
computer does not have an RS-232 port, a Tripp Lite®
connector is included and can be used to connect the
Maxwell®16 MDx Instrument via a USB port on the
computer using the adaptor attached to the RS-232 cable.
The USB connector port is for a bar code reader and only
allows bar code data to be imported into the Maxwell®16
MDx Instrument. Connect the USB plug from the bar code
reader to the instrument using this port. Data cannot be
exported from this connector port.
Note: Do not link a computer or printer to the USB port.
The SD Card slot is used to update firmware. An SD Card is
supplied with the Maxwell®16 MDx Instrument. This card
can be used to transfer firmware to the Maxwell®16 MDx
Instrument from a computer (see Section 10.C). The SD
card slot cannot be used to download data from the
Maxwell®16 MDx Instrument. We recommend that you
keep the SD Card in the instrument to avoid misplacing it.
Figure 6 shows the hardware components inside the
machine. The magnetic rod assembly varies depending on
the configuration (SEV or LEV). Figure 6, Panel A, shows
the SEV hardware configuration indicating the slides, which
allow the plunger to move up and down; the magnetic rod
assembly, which holds the magnets in place; and the plunger
bar, which contains the magnetic rods. Figure 6, Panel B,
shows the LEV hardware configuration that incorporates an
adaptor to hold the LEV magnetic rods and LEV plunger
bar. Figure 6, Panel C, shows the platform indicating the
slots where the SEV cartridges and elution tubes are placed.
For LEV, the cartridge sample rack containing the LEV
cartridges is placed on the platform (rack not shown).
Linear slides
Linear slides
Standard magnetic rods
Plunger bar
LEV magnetic rods
Plunger bar
Magnetic rod
assembly
C. Maxwell®16 Platform
Heated elution
tube slots
Reagent
cartridge slots
5182TD
A. Standard Elution Volume (SEV) Configuration
B. Low Elution Volume (LEV) Configuration
LEV plunger bar
adaptor
Figure 6. Magnetic assemply components and
platform.

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9514TB
Figure 7. Orientation of Magnetic Rod Assembly. The orientation of the magnetic rod assembly, with the
screw holes facing the back of the instrument.
Figure 7 shows the correct orientation of the magnetic rod assembly. The screw holes face the back, and the
magnetic rods sit toward the front of the instrument. If the rod assembly needs to be removed for cleaning
(see Section 8) and replaced, ensure that this orientation is retained.
3. Hardware Mode
The hardware mode chosen for each run depends on the purification protocol being used. Please refer to the
Technical Bulletin or Manual for the various Maxwell®Purification Kits for information on the hardware mode
setting required.
The existing hardware mode (SEV or LEV) setting is displayed in the upper right hand corner of the Home screen.
The instrument displays the hardware mode entered but does not sense the hardware installed. Always verify that
the hardware installed matches what is displayed on the Home screen. Make sure that the hardware and hard-
ware mode are appropriate for the purification kit being used. If the installed hardware is not correct for the kit
being used, remove the installed magnetic rod assembly and replace it with the appropriate magnetic rod assembly
(see Section 7.A).
If the installed hardware does not match the hardware mode displayed, turn the machine off, and install the
appropriate hardware with the machine turned off. When the machine is powered up after the installation, the
installed hardware will match the hardware mode displayed on the Home screen.
Back
Front

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4. Unpacking and Setting Up the Maxwell®16 MDx Instrument
4.A. Unpacking
1. Remove the accessory box, power cord and literature package from the shipping container. Slide the instru-
ment out of the box. Note: Do not lift the instrument out of the box by the door handle.
2. Remove the foam packaging from the sides of the instrument, and remove the clear plastic cover.
3. Check that all the parts have been included (Figure 1).
4. Set the Maxwell®16 MDx Instrument on a flat, level, solid surface in a dust-free location with good air
circulation. If possible, move the instrument back from the edge of the surface to prevent inadvertently
bumping the open door.
Important. Save the packaging material in case the instrument needs to be returned for service or repair
later.
4.B. Removing the Magnetic Rod Assembly/Plunger Bar Platform Shipping Anchors
1. Ensure that the instrument is turned offand unplugged.
2. The magnetic rod assembly, plunger bar and platform are anchored in place during shipment to prevent
movement of and damage to these parts.
Note: If the instrument has been switched on before removing the shipping anchors, you will hear the
motors grinding as they attempt to perform instrument calibration. If this occurs, immediately turn off
andunplug the instrument and remove the shipping anchors. This will not result in permanent damage to
the instrument.
3. Open the instrument door and locate the Magnetic Rod Assembly/Plunger Bar and Platform Shipping
Anchor thumbscrews. (Figures 8 and 9). Remove them.
Important. Save the shipping anchor thumbscrews in case the instrument needs to be moved or returned
for service or repair at a later date.
Upper shipping anchors
6922TA
Figure 8. Upper shipping anchors.
!
!

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8249TA
Magnetic Rods
Platform
Anchors
Plunger Bar
4.C. UV Bulb Installation
To prevent damage to the UV bulb, wear gloves—do not touch the bulb with bare hands. Oil from your hands can
compromise the bulb and shorten its life span. Remove the UV bulb from the box and wrapping. The bulb may
rattle if shaken; this is normal. Locate the UV lamp socket, which is on the ceiling of the instrument just inside the
door. Slide the bulb into the socket (see Figure 10). Twist the bulb a quarter turn until it is firmly in place. The UV
bulb has an average life of 3,000 hours. The Maxwell®16 MDx Instrument will warn you when the accumulated
UV bulb usage reaches 2,950 hours, and we recommend that you replace the bulb at that point. Replacement
bulbs can be purchased from Promega (Cat.# SP1080).
To replace the bulb, locate the old bulb in the UV lamp holder on the ceiling of the instrument just inside the door
(see Figure 11). Wear gloves—do not touch the bulb with your bare hands. Twist the bulb a quarter turn until it
can slide out. Place the new bulb into UV lamp socket and twist a quarter turn until it is firmly in place.
Your Maxwell®16 MDx Instrument is now ready for operation.
8086TA
Figure 9. Platform shipping anchors.
Figure 10. UV bulb in the power supply.

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4.D. Maxwell®Sample Track Software
To use the Maxwell®Sample Track Software, you need to load the software onto a computer that will be connected
to the Maxwell®16 MDx Instrument. See the Technical Manual supplied with the software for more information.
To install the software, place the CD containing the Maxwell®Sample Track Software in the CD drive of your
computer, and follow the on-screen instructions. During installation the Maxwell®Sample Track program will
check to see if Microsoft® Windows®Installer Version 3.1 and Microsoft®.NET Framework Version 2.0 are
installed on your computer. If necessary, the Maxwell®Sample Track installer will prompt you to install these
applications, which are supplied on the Maxwell®Sample Track CD. Once the software is loaded, connect the
computer to the RS-232 port of the Maxwell®16 MDx Instrument using the RS-232 cable. Do not connect your
computer to the USB port on the Maxwell®16 MDx Instrument.If your computer does not have a serial
port, use the supplied Tripp Lite®adaptor to connect the USB port of the computer to the RS-232 cable. To use
the Tripp Lite®adaptor, you must first load the driver from the minidisk in the Tripp Lite®box. Place the minidisk
in the CD drive of your computer and follow the instructions supplied in the Tripp Lite®instruction manual.
Connect the bar code reader to the USB port on the side of the instrument.
Users who wish to print run data directly can connect a serial printer to the RS-232 port of the Maxwell®16 MDx
Instrument and print (see Section 6).
4.E. Operational Setup
Using the Touch Screen
Note: In the instructions below, screen names are presented in bold type, and on-screen options are presented in
italics.
The Maxwell®16 MDx touch screen allows the user to select options. Most screens contain a series of navigation
buttons that allow the user to scroll up and down easily between options. The touch screen buttons are the same as
those on the keypad (see Figure 3), and they perform the same functions. Selection may be made using either set
of buttons. The Run/Stop button is located on the bottom right of the touch screen.
Switching on the Instrument
Once the shipping anchors and all the packaging materials have been removed, the UV bulb installed and the
peripherals attached, you can connect the instrument to a power outlet. Ensure that the On/Offswitch is in the Off
position. The power switch is located next to the power cord connection on the back of the instrument. Connect
the power cord to the back of the Maxwell®16 MDx Instrument, and plug the power cord into a wall outlet. See
Section 1.E for power requirements. Switch instrument on using the On/Offswitch.
Every time the instrument is powered on, it will perform a self-diagnostic test before opening the Home screen.
The platform, plunger bar and magnetic rod assembly are moved to check operation, and the UV light is briefly
switched on to ensure that the bulb is functioning.

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Shut Down Procedure:
Switch the instrument Offusing the On/Offswitch. Unplug the machine. If you need to store the instrument,
switch the instrument Off, unplug the instrument and store in a place that meets the environmental requirements
described in Section 1.E.
Configuring the Instrument Using the Maxwell® Wizard
The Maxwell® Wizard starts the first time the instrument is switched on and guides you through the initial setup.
This Wizard will help you to set up the instrument configuration to best meet your needs. During setup you can set
the following parameters: Language, Touch Screen settings, Date/Time, UV Lamp run options, Sample Tracking,
Instrument Name and Approved Users. Settings can be changed later if required (see Section 7.B).
The Wizard runs through the following option screens. You can select the appropriate option as needed.
1. Language. The default language is English. If you wish to change the language, choose from the list in the
Select Languages screen. You may need to scroll down to the desired language. Once you have selected a
language, press the Run/Stop button.
8285T
A
Figure 11. The Select Languages screen. Once you have
selected a language, press the forward arrow button.

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4.E. Operational Setup (continued)
2. Touch Screen. You will need to calibrate the TouchScreen after shipping.
8205T
A
8206TA
The “Touch Screen Reset Successful” message indicates that the calibration was successful, and the Wizard can
continue.
Figure 12. Calibrating the Touch Screen. To calibrate the screen
touch the “+” symbols in the order they are presented. A confirmation
screen will open.
Figure 13. Confirmation screen for the Touch screen. Touch all
four boxes; if they all change color, press the Stop/Run button on the
instrument keypad. If they do not all change color, press the back arrow
button and repeat the calibration and confirmation.

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3. Date/Time. Select the Date/Time screen to set the current date and time. In the initial screens, select
the date and time formats. In the final Date/Time Setup screen, set the current time and date. Selecting
each of the screen options opens a keypad to add numerical values.
8207T
A
Figure 14. Date/Time Setup screen.
4. UV Lamp. You can program the Maxwell®16 MDx Instrument to turn on the UV lamp automatically. It can
be set up to turn on after each run or at start up, or it can be set to remain off. The treatment time can be set
in increments of 10 minutes for up to 10 hours. The UV lamp also can be turned on from the Setup menu
(see Section 8.D).
8208T
A
Figure 15. UV Settings screen. Select the desired option
on the UV screen, and then set the time for UV treatment.