Pyxis SP-910 Reference guide

SP-910 Portable Water Analyzer
Procedures Manual
Rev. A
Firmware version 1.0.170
Pyxis Lab, Inc 2016
All Rights Reserved

目录
Fluorescence—Fluorescein................................................................................................. 2
Fluorescence—PTSA.......................................................................................................... 6
Turbidity ............................................................................................................................. 10
ALUMINUM —AL.............................................................................................................. 14
BENZOTRIAZOLE—AZOL................................................................................................ 18
Calcium—Ca...................................................................................................................... 22
CHLORINE, TOTAL, Ultra-High Range —CL2H.............................................................. 26
CHLORINE,FREE—CL-F.................................................................................................. 30
CHLORINE DIOXIDE—CLO2........................................................................................... 34
CHLORINE DIOXIDE,Mid Range —CLO2D..................................................................... 38
CHLORINE,TOTAL—CL-T................................................................................................ 42
CYANIDE—CN .................................................................................................................. 46
CHROMIUM, Hexavalent—Cr6......................................................................................... 51
CHROMIUM, TOTAL—CrT................................................................................................ 55
COPPER—CuBi ................................................................................................................ 59
COPPER—CuL.................................................................................................................. 63
CYANURIC ACID —CYAN ............................................................................................... 67
FLUORIDE —F................................................................................................................. 71
Total Iron—FeMo ............................................................................................................... 75
Total Iron—FePh................................................................................................................ 79
Total Iron—FeTp................................................................................................................ 83
Total Iron—FeZi ................................................................................................................. 87
Magnesium—Mg................................................................................................................ 91
MANGANESE,High Range—MnHR.................................................................................. 95
MANGANESE, Low Range—MnLR.................................................................................. 99
Molydemum,High Range—MoHR................................................................................... 103
Molydemum,Low Range—MoLR..................................................................................... 107
HYDRAZINE—N2H4....................................................................................................... 111
CHLORAMINE, MONO, Low Range—NH2C.................................................................. 115
NITROGEN,AMMONIA—NH3S...................................................................................... 119
NICKEL—Ni..................................................................................................................... 123
NITRITE, High Rang—NO2H.......................................................................................... 127
NITRITE, Low Rang—NO2L............................................................................................ 131
NITRATE, High Range—NO3HR.................................................................................... 135
NITRATE, Mid Range—NO3MR...................................................................................... 139
PHOSPHORUS, Reactive—OPO4 ................................................................................. 143
PHOSPHONATES—Orgp ............................................................................................... 147
PHOSPHORUS, REACTIVE—PAMI............................................................................... 152
POTENTIAL OF HYDROGEN—PH ................................................................................ 156
PHOSPHORUS, REACTIVE—PMOV............................................................................. 160
POLYMER—POLY........................................................................................................... 164
SULFIDE —S2-................................................................................................................ 168

SILICA , High Range—SiHR............................................................................................ 172
SILICA , Low Range—SiLR............................................................................................. 176
SULFATE—SO4 .............................................................................................................. 181
ZNIC—Zn......................................................................................................................... 185

2
Fluorescence—Fluorescein
SP-910 Test Method
Testing Program
Description：SP-910 PTSA Method (0.1 –20.0 ppb Fluorescein)
(Fluorescent tracer Fluorescein measurement)
Instruments and Reagents：
1. SP-910 Portable Water Analyzer
2. 10mL Sample Vial（SP-910,Associated Sample Vial）
Program
1. Press OK key on the navigation control panel for 3 seconds until
the screen lights up. On the main page, the screen will display
eight major feature groups .the main menu options.
2. Move the icon focus to M-F icon using the navigational (left, right,
up, or down) keys.
3. Fill the 10 ml sample vial with the test solution and tightly cap the
sample vial. Use a soft cloth or lint free paper tissue to clean the
sample vial.
4. Place the sample vial into the sample vial compartment and slide
the light shield cover to the closed position.
5. Press the M-F on the main page. Pyxis SP-910 will start to
measure the fluorescein concentration in the sample.
6. Pyxis SP-910 will display the fluorescein concentration in ppb as
fluorescein.
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Power
M-F TURB
ABSB SYSCAL
COLOR
M-P

3
For best results, ensure that the sample vial is clean. Wipe off water on the outside
wall of the sample vial using a lint-free tissue paper. Fill the sample vial to the 10
ml mark. If the sample contains air bubbles, tap the sample vial gently to remove
the bubbles before placing the sample vial to sample vial compartment.
Fluorescein calibration
1. Deionized water (DI) as the blank calibration solution and the 20
ppb fluorescein calibration standard solution are needed.
2. Press the CAL on the main page, then choose the M-F and press
the ok button to launch the fluorescein calibration page.
3. Follow the message prompts, insert the DI blank into the sample
vial compartment and press the OK key measure the deionized
water.
4. Follow the message prompts, and insert the 20 ppb standard into
the sample vial compartment and press the OK key to measure
the 20 ppb standard.
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M-F
19.8 ppb
Click OK key to measure,
Click other keys to exit!
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M-F Calibration
Insert 0.0 ppb standard!
Click any key to continue!

4
5. Press the OK key to save the paraments.
6. Press the OK key to return to the main page
The 20 ppb standard solution shall be stored in a brown or black opaque bottle. Exposing
the fluorescein standard to light will cause the standard losing the fluorescein
concentration. Many substances, such as quaternary amine cause a negativeinterference.
Many other substances suchlaundry detergents that contain optical brightener will cause
a significant positive interference
7. If calibration fails, the followings should be checked:
The DI blank is being contaminated.
The 20 ppb fluorescein standard solution is decayed or being contaminated.
The light shield cover is not in the closing position.
The sample vial compartment is blocked with debris, water, or other materials.
NOTES:
1. The center key is the OK key. Press the OK key on a selected item
to launch the action associated with the selected item.
2. Rinse all glassware with 1:1 Hydrochloric Acid Solution. Rinse
again with deionized water.
3. When the sample vial is inserted into the sample vial compartment,
the triangular mark on the sample vial should be aligned
approximately with the 6 o’clock position of the sample vial
compartment or any position consistently.
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M-F Calibration
Insert 0.0 ppb standard!
7730
Insert 20.0 ppb standard!
Click OK key to continue!
Click up and down key to change!
2016/07/19 08:00
M-F Calibration
Insert 0.0 ppb standard!
7730
F1=0.00063
Calibration Succeeded!
Click OK key to accept,
Other keys to cancel!

5
4. Pyxis SP-910 automatically turns itself off after 2 minutes with no-
key activity, except for during a measurement. Pressing and
holding the OK key for 3 seconds will wake up the instrument, and
return to the original page if it has any measurement data.

6
Fluorescence—PTSA
SP-910 Test Method
Testing Program
Description：SP-910 PTSA Method (1.0 –300.0 ppb PTSA)
(Fluorescent tracer PTSA measurement)
Instruments and Reagents：
1. SP-910 Portable Water Analyzer
2. 10mL Sample Vial（SP-910,Associated Sample Vial）
Program
1. Press OK key on the navigation control panel for 3 seconds until
the screen lights up. On the main page, the screen will display
eight major feature groups .the main menu options.
2. Move the icon focus to M-P icon using the navigational (left, right,
up, or down) keys.
3. Fill the 10 ml sample vial with the test solution and tightly cap the
sample vial. Use a soft cloth or lint free paper tissue to clean the
sample vial.
4. Place the sample vial into the sample vial compartment and slide
the light shield cover to the closed position.
5. Press the M-P on the main page. Pyxis SP-910 will start to
measure the PTSA concentration in the sample.
6. Pyxis SP-910 will display the PTSA concentration in ppb as PTSA.
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Power
M-F TURB
ABSB SYS
M-P
CAL
COLOR

7
During the fluorescence measurement to determine the PTSA concentration, Pyxis
SP-910 checks the sample turbidity. If the sample turbidity value detected is
greater than 40 NTU, Pyxis SP-910 will display a warning. For best results, the
sample should be filtered if turbidity exceeds 40 NTU.
Sample color causes a lower PTSA concentration to be measured. Pyxis SP-910
automatically compensates for sample color. If the sample color is too intense,
Pyxis SP-910 will display a warning.
For best results, ensure that the sample vial is clean. Wipe off water on the outside
wall of the sample vial using a lint-free tissue paper. Fill the sample vial to the 10
ml mark. If the sample contains air bubbles, tap the sample vial gently to remove
the bubbles before placing the sample vial to sample vial compartment.
PTSA calibration
1. Deionized water (DI) as the blank calibration solution and the 100
ppb PTSA calibration standard solution are needed.
2. Press the CAL on the main page, then choose the M-P and press
the ok button to launch the PTSA calibration page.
3. Follow the message prompts, insert the DI blank into the sample
vial compartment and press the OK key measure the deionized
water.
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PTSA
99.8 ppb
Click OK key to measure,
Click other keys to exit!
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PTSA Calibration
Insert 0.0 ppb standard!
Click any key to continue!

8
4. Follow the message prompts, and insert the 100 ppb standard into
the sample vial compartment and press the OK key to measure
the 100 ppb standard.
5. Press the OK key to save the paraments.
6. Press the OK key to return to the main page
The100 ppb standard solutionshall bestored in abrownorblack opaquebottle. Exposing
the PTSA standard to light will cause the standard losing the PTSA concentration. Many
substances, such as quaternary amine cause a negative interference. Many other
substances suchlaundry detergents that contain optical brightener will cause a significant
positive interference
7. If calibration fails, the followings should be checked:
The DI blank is being contaminated.
The 100 ppb PTSA standard solution is decayed or being contaminated.
The light shield cover is not in the closing position.
The sample vial compartment is blocked with debris, water, or other materials.
NOTES:
1. The center key is the OK key. Press the OK key on a selected item
to launch the action associated with the selected item.
2. Rinse all glassware with 1:1 Hydrochloric Acid Solution. Rinse again
with deionized water.
3. When the sample vial is inserted into the sample vial compartment,
the triangular mark on the sample vial should be aligned
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PTSA Calibration
Insert 0.0 ppb standard!
39875, 385, 18705, 7920
Insert 100.0 ppb standard!
Click OK key to continue!
Click up and down key to change!
2016/07/19 08:00
PTSA Calibration
Insert 0.0 ppb standard!
39875, 385, 18750, 7920
f1=1.958
Calibration Succeeded!
Click OK key to accept,
Other keys to cancel!

9
approximately with the 6 o’clock position of the sample vial
compartment or any position consistently.
4. Pyxis SP-910 automatically turns itself off after 2 minutes with no-
key activity, except for during a measurement. Pressing and
holding the OK key for 3 seconds will wake up the instrument, and
return to the original page if it has any measurement data.

10
Turbidity
SP-910 Test Method
Test Program
Description：SP-910 Turbidity Method (1.0 –500.0 NTU) (Turbidity
Method)
Instruments and Reagents：
1. SP-910 Portable Water Analyzer
2. 10mL Sample Vial(SP-910,Associated Sample Vial)
Program
1. Press OK key on the navigation control panel for 3 seconds until
the screen lights up. On the main page, the screen will display
eight major feature groups the main menu options.
2. Move the cursor to Turb icon using the navigational (left, right, up,
or down) keys.
1 FAU=1 NTU=1 FTU
3. Fill the 10 ml sample vial with the test solution and tightly cap the
sample vial. Swirl the vial. Use a soft cloth or lint free paper tissue
to clean the sample vial.
4. Place the sample vial into the sample vial compartment and slide
the light shield cover to the closed position.
Mix the sample well before transferring it to the sample vial compartment
5. Press the Turb on the main page. Pyxis SP-910 will start to
measure the Turbiditty concentration in the sample.
6. Pyxis SP-910 will display the Turbidity concentration in NTU as
turbidity.
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Power
M-F TURB
ABSB SYSCAL
COLOR
M-P

11
Collect samples in clean plastic or glass bottles. Analyze samples as soon as
possible. Store samples up to 48 hours by cooling to 4°C (39 °F). Analyze the
sample at the same temperature as it was collected.
If the sample contains air bubbles, tap the sample vial gently to remove the
bubbles before placing the sample vial to sample vial compartment.
Turbidity calibration
1. Deionized water (DI) as the blank calibration solution and the 100
100NTU /400NTU formazin calibration standard solution are
needed.
2. Press the CAL on the main page, then choose the Turb and press
the ok button to launch the turbidity calibration page.
3. Follow the message prompts, insert the DI blank into the sample
vial compartment and press the OK button to measure the
deionized water.
4. Follow the message prompts, and insert the 100 NTU formazin
standard into the sample vial compartment and press the OK
button to measure the 100 NTU standard.
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Turbidity
19.8 NTU
Click OK key to measure,
Click other keys to exit!
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Turb Calibration
Insert 0.0 NTU standard!
Click any key to continue!

12
5. Press the OK key to contintue high range turbidity calibration. If
high range turbidity calibration not required,press any keys to exit.
6. Fill the 10 ml sample vial to above 10 ml mark with the 400 NTU
formazin standard. Insert the sample vial to the sample vial
compartment.
7. Press the OK key to measure the 400 NTU standard. High range
turbidity calibration is successful.
8. Press any keys to exit.
9. If calibration fails, the followings should be checked:
The DI blank is being contaminated.
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Turb Calibration
Insert 0.0 NTU standard!
1358
Insert 100.0 NTU standard!
Click OK key to continue!
Click up and down key to change!
2016/07/19 08:00
Turb Calibration
Insert 0.0 NTU standard!
1358
Insert 100.0 NTU standard!
48809
Calibration Succeed!
Click OK key to continue,
Other keys to exit!
2016/07/19 08:00
Turb Calibration
Insert 100.0 NTU standard!
8577
Insert 400.0 NTU standard!
Click OK key to continue!
Click up and down key to change!
2016/07/19 08:00
Turb Calibration
Insert 100.0 NTU standard!
8577
Insert 400.0 NTU standard!
47890
Calibration succeeded!
Click any key to exit!

13
The 100 NTU/400NTU formazin standard solution is decayed or being
contaminated.
The light shield cover is not in the closing position.
The sample vial compartment is blocked with debris, water, or other materials.
NOTES:
1. The center key is the OK key. Press the OK key on a selected item
to launch the action associated with the selected item.
2. Rinse all glassware with 1:1 hydrochloric acid solution. Rinse
again with deionized water.
3. When the sample vial is inserted into thesample vial compartment,
the triangular mark on the sample vial should be aligned
approximately with the 6 o’clock position of the sample vial
compartment or any position consistently.
4. Pyxis SP-910 automatically turns itself off after 2 minutes with
no-Button activity, except for during a measurement. Pressing
and holding the OK button for 3 seconds will wake up the
instrument, and return to the original page if it has any
measurement data.

14
ALUMINUM —AL
SP-910 Test Method
Test Program
Description：SP-910 Aluminum Method (0.02 –0.8 ppm AL) (Aluminon
Method)
Instruments and Reagents：
1. SP-910 Portable Water Analyzer
2 10mL Sample Vial(SP-910,Associated Sample Vial)
3. 25-ml Sample Vial
4. 50-ml Graduated Mixing Cylinder
5. Pyxis AL Reagent
Program
1. Press OK button (the centre button) on the navigation control
panel for 3 seconds until the screen lights up. on the main page,
the screen will display eight major feature groups the main menu
options.
2. Position the cursor to Mthd icon by navigation buttons and press the
OK button to enter Mthd selection interface, position the cursor to
AL icon

15
Press the OK button to enter AL test program interface.
3. Fill a 50-mL graduated mixing cylinder to the 50-mLmark with
sample.
Note: Rinse cylinder with 1:1 Hydrochloric Acid and deionized water before use
to avoid errors due to contaminants absorbed on the glass.
Note: Sample temperature must be 20-25 °C (68-77 °F) for accurate results.
4. Add the contents of one AL-1 powder pillow. Swirl the vial to mix
the reagent.
5. Add the contents of one AL-2 powder pillow. Swirl the vial to mix
the reagent.
Note: Ared-orange color develops if aluminum is present.
Note: Inconsistent results will occur if any powder is undissolved.
6. Press the TMR1 Button to start the method timer,a 3-minute reaction
period will begin. Invert the cylinder repeatedlyfor the three minutes.
7. When the timer reaches the preset time and the reaction is
complete , the timer beeps,pour 10 mL of sample into a 10-mL

16
sample vial (the prepared sample).
8. Pour 25 mL of remaining sample into a 25-mL sample vial ,add the
contents of one AL-3 regentthe to 25 mL sample vial (the blank
sample).
9. Press the TMR2 button to start the method timer,a thirty-second
reaction period will begin. vigorously shake the cylinder for the 30-
second period
Note: This solution should turn a light to medium orange upon bleaching. It will
not become colorless.
10.Press the TMR3 Button to start the method timer,a 15-minute
reaction period will begin.
11.Pour the 25 mL of mixture in the cylinder into a second 10-mL
sample cell (the blank sample).
12. When the timer reaches the preset time and the reaction is
complete , after the timer beeps, the cursor will automatically
switch to EXIT Button. Press the OK Button to the icon menu-
assisted
13.Use a soft cloth or lint free paper tissue to clean the sample vial.
14.Repeat step 2, place the prepared blank into the Pyxis SP-910
sample vial compartment and press the ZERO Button.
15.Place the prepared sample into the sample vial compartment.
Tightly cover the sample cell with the instrument cap.

17
16.Place the prepared sample into the Pyxis SP-910 sample vial
compartment and press the READ Button.
17.Concentration value based on the last absorbance value
measured will be calculated and displayed. Pyxis SP-910 will
display the page.
18.Press EXIT Button to return to the main page.
The method is compatible with HACH 8012
NOTES:
1 Rinse all glassware with 1:1 hydrochloric acid solution. Rinse again
with deionized water.
2. When the sample vial is inserted into the sample vial compartment,
the triangular mark on the sample vial should be aligned
approximately with the 6 o’clock position of the sample vial
compartment or any position consistently.
3. Pyxis SP-910 automatically turns itself off after 2 minutes with no-
Button activity, except for during a measurement. Pressing and
holding the OK Button for 3 seconds will wake up the instrument,
and return to the original page if it has any measurement data.
4. Press the CONF button in the method result page to launch the
method setup and calibration page. Press the FORM button to
select a concentration form from the list of forms
(PO4 ;PBTC;HEDP).Press the UNIT button to select a
concentration unit among the list of ppb, ppm, mg/L, and ug/L.

18
BENZOTRIAZOLE—AZOL
SP-910 Test Method
Test Program
Description：SP-910 AZOL Method (0.7 –16.0 ppm BENZO) (UV
Photolysis Method)
Instruments and Reagents：
1. SP-910 Portable Water Analyzer
2. 10mL Sample Vial(SP-910,Associated Sample Vial)
3. Ultraviolet (UV) lamp，115V，60HZ
4. UV Safety Goggles
5. 25-ml Sample Vial
6. Pyxis AZOL Reagent
Program
1. Fill a sample cell to the 25-mL mark with sample
Note: Sample temperature should be between 20-25 °C(68-77 °F).
Note: If sample contains nitrite or borax (sodium borate), adjust the pH to between 4
and 6 with 1 N sulfuric acid.
2. Add the one content of AZOL-1 regent to the 25-mL sample vial
Swirl to dissolve completely.
Note: If the sample contains more than 500 mg/L hardness (as CaCO3), add 10
drops of Rochelle Salt Solution.
3. Insert the ultraviolet(UV) lamp into the 25ml sample vial.
Note: Wear UV safety goggles while the lamp is on.
Note: Do not handle the lamp surface. Fingerprints will etch the glass. Wipe lamp
with a soft, clean tissue between samples.
Note: Aspecially designed cord adapter is available for performing two
digestions with a single power supply. Asecond UV lamp is required.
4. Turn on the UV lamp to digest the sample.
5. Press OK button (the centre Button) on the navigation control
panel for 3 seconds until the screen lights up. On the main page,
the screen will display eight major feature groups the main menu
options.