Pyxis SP-800 Reference guide

SP-800 Portable Water Analyzer
Procedures Manual
Pyxis Lab, Inc.
1729 Majestic Dr. Suite 5
Lafayette, CO 80026 USA
www.pyxis-lab.com
© 2019 Pyxis Lab, Inc.
Pyxis Lab Proprietary and Confidential

Table of Contents
REQUIRED REAGENTS...................................................................................................................... 4
1. Turbidity................................................................................................................................. 12
2. Aluminum – Al........................................................................................................................ 16
3. Alkalinity, Low Range - ALKLR ................................................................................................ 21
4. Alkalinity, High Range - ALKHR............................................................................................... 24
5. Benzotriazole/Tolyltriazole - AZOL......................................................................................... 27
6. Bleach - BLCH......................................................................................................................... 31
7. Bromine - Br-T........................................................................................................................ 34
8. Calcium - Ca ........................................................................................................................... 37
9. Calcium Hardness - CaHR....................................................................................................... 41
10. Oxygen Demand, Chemical (Reactor Digestion 2h Method) - COD................................... 44
11. Oxygen Demand, Chemical (Reactor Digestion 20 Minutes Method) - COD..................... 51
12. Chloride Medium Range - CLLR.......................................................................................... 57
13. Chloride Medium Range - CLMR........................................................................................ 60
14. Chlorine, Total, High Range - CL2H..................................................................................... 63
15. Chlorine, Free, High Range - CL2H ..................................................................................... 66
16. Chlorine, Free, Ultra-High Range - CL2UH.......................................................................... 69
17. Chlorine, Free - CL-F........................................................................................................... 72
18. Chlorine, Free - CLTMB....................................................................................................... 75
19. Chlorine Dioxide - CLO2 ..................................................................................................... 78
20. Chlorine Dioxide - CLO2D................................................................................................... 82
21. Chlorine, Total - CL-T .......................................................................................................... 85
22. Cyanide - CN....................................................................................................................... 88
23. Color, True and Apparent - COLOR..................................................................................... 93
24. Chromium, Hexavalent - Cr6 .............................................................................................. 97
25. Chromium, Total - CrT ...................................................................................................... 100
26. Copper - CuBi ................................................................................................................... 104
27. Copper - CuLR................................................................................................................... 107
28. Cyanuric Acid - CYAN........................................................................................................ 111
29. Diethyl hydroxylamine - DEHA ......................................................................................... 114
30. Fluoride - F ....................................................................................................................... 118
31. Tannin and Lignin – Ligs ................................................................................................... 121
32. Total Iron - FeMo.............................................................................................................. 125
33. Total Iron - FePh ............................................................................................................... 129
34. Total Iron - FeSal............................................................................................................... 132
35. Total Iron - FeTp................................................................................................................ 135
36. Total Iron - FeZi................................................................................................................. 138
37. Magnesium - Mg.............................................................................................................. 142

38. Manganese, High Range - MnHR ..................................................................................... 146
39. Manganese, Low Range - MnLR....................................................................................... 150
40. Molydemum, High Range - MoHR ................................................................................... 154
41. Molybdenum, Low Range - MoLR.................................................................................... 158
42. Hydrazine - N2H4 ............................................................................................................. 162
43. Chloramine, Mono, Low Range - NH2C............................................................................ 165
44. Nitrogen, Total (Test ‘N Tube Method) - N-TLR................................................................ 168
45. Nitrogen, Total (Test ‘N Tube Method) - N-THR ............................................................... 173
46. Nitrogen, Ammonia - NH3S.............................................................................................. 178
47. Nitrogen, Ammonia (Test ‘N Tube) - NH3LR..................................................................... 182
48. Nitrogen, Ammonia (Test ‘N Tube) - NH3HR.................................................................... 186
49. Nickel - Ni ......................................................................................................................... 190
50. Nitrogen Dioxide - NO2D.................................................................................................. 194
51. Nitrite, High Rang - NO2HR.............................................................................................. 197
52. Nitrite, Low Rang - NO2LR................................................................................................ 200
53.Nitrate, High Range - NO3HR ........................................................................................... 203
54. Nitrate, Mid-Range - NO3MR........................................................................................... 207
55. Nitrate, High Range (Test ‘N Tube Method) - NO3CA ...................................................... 211
56. Peroxyacetic - PAA............................................................................................................ 214
57. Phosphorus, Reactive - OPO4 .......................................................................................... 217
58. Phosphonates - OrgP........................................................................................................ 220
59. Phosphorus, Reactive - Pami............................................................................................ 225
60. Phosphorus, Total (Test ‘N Tube Method) - P-TLR............................................................ 228
61. Phosphorus, Total (Test ‘N Tube Method) - P-THR........................................................... 231
62. Potential of Hydrogen - pH............................................................................................... 234
63. Phosphorus, Reactive - PMoV.......................................................................................... 237
64. Polymer - POLY................................................................................................................. 240
65. Antimony Trivalent - Sb3+................................................................................................ 243
66. Antimony, Total - Sb-T ...................................................................................................... 246
67. Sulfide - S2-...................................................................................................................... 249
68. Silica, High Range - SiHR................................................................................................... 252
69. Silica, Low Range - SiLR .................................................................................................... 256
70. Sulfite, Low Range - SO3LR .............................................................................................. 260
71. Sulfite, High Range - SO3HR............................................................................................. 263
72. Sulfate - SO4..................................................................................................................... 266
73. Urea (Reactor Digestion Method) - Urea ......................................................................... 269
74. Zinc - ZnXO ....................................................................................................................... 272
75. Zinc - Zn............................................................................................................................ 275

Pyxis SP-800 Analyzer Procedures Manual 4
REQUIRED REAGENTS
Method
Required Reagents
Cat. No.
Includes
Cat. No.
AL
Aluminum Reagent
22420-00
AluVer 3 Aluminum Reagent
Powder Pillow
14290-99
Ascorbic Acid Powder Pillow
14577-99
Bleaching 3 Reagent Powder
Pillow
14294-49
AZOL
Triazole Reagent
Powder Pillows
21412-99
N/A
N/A
Br-T
DPD Total Chlorine
Reagent Powder
Pillows
21056-69
N/A
N/A
Ca
Hardness Reagent
Set
23199-00
Alkali Solution for Calcium and
Magnesium Test
22417-32
Calcium and Magnesium
Indicator Solution
22418-32
EDTA Solution, 1 M
22419-26
EGTA Solution
22297-26
CODLR
CODLR Reagent
21258-25
Low Range, 0 to 150 mg/L COD
N/A
CODHR
CODHR Reagent
21259-25
High Range, 0 to 1,500 mg/L
COD
N/A
COD High Range
Plus Reagent
24159-25
High Range Plus, 0 to 15,000
mg/L COD
N/A
CODLF
CODLF Reagent
2038225
Low Range, 0 to 150 mg/L COD
N/A
CODHF
CODHF Reagent
2038325
High Range, 0 to 1,500 mg/L
COD
N/A
CL2H
DPD Total Chlorine
Reagent Powder
Pillows
14064-99
N/A
N/A
CL2H
DPD Free Chlorine
Reagent Powder
Pillows
14070-99
N/A
N/A
CL-F
DPD Free Chlorine
Powder Pillows
21055-69
N/A
N/A
CLO2
Chlorine Dioxide
DPD/Glycine
Reagent Set
27709-00
DPD Free Chlorine Reagent
Powder Pillows
21055-69
Glycine Reagent
27621-33

Pyxis SP-800 Analyzer Procedures Manual 5
Method
Required Reagents
Cat. No.
Includes
Cat. No.
CLO2D
Direct Reading
N/A
N/A
N/A
CL-T
DPD Total Chlorine
Reagent Powder
Pillows
21056-69
N/A
N/A
CN
Cyanide Reagent
Set
24302-00
CyaniVer 3 Cyanide Reagent
Powder Pillows
21068-69
CyaniVer 4 Cyanide Reagent
Powder Pillows
21069-69
CyaniVer 5 Cyanide Reagent
Powder Pillows
21070-69
COLOR
Aspirator, vacuum
2131-00
N/A
N/A
Filter Holder, 47
mm, 300 mL
graduated
13529-00
N/A
N/A
Filter, membrane,
47 mm, 0.45
microns
13530-00
N/A
N/A
Flask, filtering, 500
mL
546-49
N/A
N/A
Stopper, No. 7, one
hole
2119-07
N/A
N/A
Cr6+
ChromaVer 3
Chromium Reagent
Powder Pillows
12710-99
N/A
N/A
CrT
Total Chromium
Reagent Set
22425-00
Acid Reagent Powder Pillows
2126-99
ChromaVer 3 Chromium
Reagent Powder Pillows
12066-99
Chromium 1 Reagent Powder
Pillows
2043-99
Chromium 2 Reagent Powder
Pillows
2044-99
CuBi
CuVer 1 Copper
Reagent Powder
Pillows
21058-69
N/A
N/A

Pyxis SP-800 Analyzer Procedures Manual 6
Method
Required Reagents
Cat. No.
Includes
Cat. No.
CuL
Copper Reagent Set
26033-00
Copper Masking Reagent
Powder Pillows
26034-49
Porphyrin 1 Reagent Powder
Pillows
26035-49
Porphyrin 2 Reagent Powder
Pillows
26036-49
CYAN
Cyanuric Acid 2
Reagent Powder
Pillow
2460-66
N/A
N/A
DEHA
DEHA Reagent Set
24466-00
DEHA Reagent 1 Powder Pillow
21679-69
DEHA Reagent 2 Powder Pillow
21680-42
F
SPADNS Reagent
for Fluoride
444-49
N/A
N/A
Ligs
Tannin and Lignin
Reagent Set
2244600
Sodium Carbonate Solution
67549
TanniVer™ 3 Tannin-Lignin
Reagent
256032
FeMo
FerroMo Reagent
Set
25448-00
FerroMo Iron Reagent 1 Powder
Pillows
25437-68
FerroMo Iron Reagent 2 Powder
Pillows
25438-66
FePh
FerroVer Iron
Reagent Powder
Pillows
21057-69
N/A
N/A
FeTp
TPTZ Iron Reagent
Powder Pillows
26087-99
N/A
N/A
FeZi
FerroZine Iron
Reagent Solution
Pillows
2301-66
N/A
N/A
Mg
Hardness Reagent
Set
23199-00
Alkali Solution for Calcium and
Magnesium Test
22417-32
Calcium and Magnesium
Indicator Solution
22418-32
EDTA Solution, 1 M
22419-26
EGTA Solution
22297-26
MnHR
High Range
Manganese
Reagent Set
24300-00
Buffer Powder Pillows, citrate
type for Manganese
21076-69
Sodium Periodate Powder
Pillows for Manganese
21077-69

Pyxis SP-800 Analyzer Procedures Manual 7
Method
Required Reagents
Cat. No.
Includes
Cat. No.
MnLR
Manganese
Reagent Set
26517-00
Alkaline-Cyanide Reagent
21223-26
Ascorbic Acid Powder Pillows
14577-99
PAN Indicator Solution, 0.1%
21224-26
MoHR
Molybdenum
Reagent Set
26041-00
MolyVer 1 Reagent Powder
Pillows
26042-99
MolyVer 2 Reagent Powder
Pillows
26043-99
MolyVer 3 Reagent Powder
Pillows
26044-99
MOLR
Molybdenum
Reagent Set
24494-00
Molybdenum 1 Reagent for 20
mL sample size
23524-49
Molybdenum 2 Reagent
Solution
23525-12
N2H4
HydraVer 2
Hydrazine Reagent
1790-32
N/A
N/A
NH2C
Monochlor F
Reagent Pillows
28022-46
N/A
N/A
N-TLR
Test ’N Tube Total
Nitrogen Reagent
Set
26722-45
TN Reagent C Vials, Acid
Solution*
26721-45
TN Hydroxide Reagent Sample
Digestion Vials*
26717-45
TN Persulfate Reagent Powder
Pillows
26718-49
TN Reagent A, Bisulfite Powder
Pillows
26719-49
TN Reagent B, Indicator Powder
Pillows
26720-49
N-THR
Test ’N Tube HR
Total Nitrogen
Reagent Set
27141-00
HR Total Nitrogen Hydroxide
Digestion Vials.
N/A
Total Nitrogen Persulfate
Reagent Powder Pillows
26718-46
Total Nitrogen Reagent A,
Bisulfite Powder Pillows
26719-46
Total Nitrogen Reagent B,
Indicator Powder Pillows
26720-46
Total Nitrogen Reagent C Vials,
Acid Solution
N/A

Pyxis SP-800 Analyzer Procedures Manual 8
Method
Required Reagents
Cat. No.
Includes
Cat. No.
NH3S
Ammonia Nitrogen
Reagent Set for 10-
mL samples
26680-00
Ammonia Cyanurate Reagent
Powder Pillows
26531-99
Ammonia Salicylate Reagent
Powder Pillows
26532-99
NH3LR
AmVer Reagent Set
for Nitrogen,
Ammonia, Low
Range TNT
26045-45
AmVer Diluent Reagent, Low
Range Test ‘N Tube
N/A
Salicylate Reagent Powder
Pillows, 5 mL sample
23952-66
Cyanurate Reagent Powder
Pillows, 5 mL sample
23954-66
NH3HR
AmVer™ Reagent
Set for Nitrogen,
Ammonia, High
Rangee, TNT
26069-45
AmVer™ HR Reagent Test ‘N
Tube™ Vials
N/A
Ammonia Salicylate Reagent
Powder Pillows
23952-66
Ammonia Cyanurate Reagent
Powder Pillows
23954-66
Ni
Nickel Reagent Set,
25 mL sample
22426-00
EDTA Reagent Powder Pillows
7005-99
Phthalate-Phosphate Reagent
Powder Pillows
21501-66
P.A.N. Indicator Solution, 0.3%
21502-32
NO2HR
NitriVer 2 Nitrite
Reagent Powder
Pillows
21075-69
N/A
N/A
NO2LR
NitriVer 3 Nitrite
Reagent Powder
Pillows
21071-69
N/A
N/A
NO3HR
NitraVer 5 Nitrate
Reagent Powder
Pillows
21061-69
N/A
N/A
NO3MR
NitraVer 5 Nitrate
Reagent Powder
Pillows
21061-69
N/A
N/A

Pyxis SP-800 Analyzer Procedures Manual 9
Method
Required Reagents
Cat. No.
Includes
Cat. No.
NO3CA
NitraVer Χ Nitrate,
High Range Test ‘N
Tube Reagent Set
26053-45
Nitrate Pretreatment Solution
Vials
N/A
NitraVer Χ Reagent B Powder
Pillows
26055-46
OPO4
PhosVer 3
Phosphate Reagent
Powder Pillows
21060-69
N/A
N/A
Orgp
Phosphonates
Reagent Set
24297-00
PhosVer 3 Phosphate Reagent
Powder Pillows
21060-69
Potassium Persulfate Pillow for
Phosphonate
20847-69
PAMI
High Range
Reactive
Phosphorus
Reagent Set
22441-00
Amino Acid Reagent
1934-32
Molybdate Reagent
2236-32
P-TLR
Total Phosphorus
Test ’N Tube
Reagent Set
27426-45
PhosVer 3 Phosphate Reagent
Powder Pillows
21060-46
Potassium Persulfate powder
Pillows
20847-66
Sodium Hydroxide Solution,
1.54 N
27430-42
Test ‘N Tube Acid Dilution Vials
N/A
P-THR
Total High Range
Phosphorus Test ’N
Tube™ Reagent Set
27672-45
Molybdovanadate Reagent
20760-26
Potassium Persulfate Powder
Pillows
20847-66
Sodium Hydroxide Solution,
1.54 N
27430-42
Total Phosphorus Test ’N Tube™
Vials
N/A
PH
Dropper, 0.5&1.0
mL marks
21247-20
N/A
N/A
Phenol Red
Indicator Solution,
spec grade
26575-12
N/A
N/A
PMOV
Molybdovanadate
Reagent
20760-32
N/A
N/A

Pyxis SP-800 Analyzer Procedures Manual 10
Method
Required Reagents
Cat. No.
Includes
Cat. No.
S2-
Sulfide Reagent Set
22445-00
Sulfide 1 Reagent
1816-32
Sulfide 2 Reagent
1817-32
SiHR
High Range Silica
Reagent Set
24296-00
Acid Reagent Powder Pillows for
High Range Silica
21074-69
Citric Acid Powder Pillows
21062-69
Molybdate Reagent Powder
Pillows for HR Silica
21073-69
SiLR
Low Range Silica
Reagent Set
24593-00
Amino Acid F Reagent Powder
Pillows
22540-69
Citric Acid Powder Pillows
21062-69
Molybdate 3 Reagent
1995-26
SO4
SulfaVer 4 Sulfate
Reagent Powder
Pillows
21067-69
N/A
N/A
Zn
Zinc Reagent Set
24293-00
Cyclohexanone
14033-32
ZincoVer 5 Reagent Powder
Pillows
21066-69
PYXIS REAGENTS
Method
Required Reagents
PN
Includes
PN
ALKLR/
ALKHR
ALKLR Reagent Set
31068
ALK-1
N/A
ALK-2
N/A
Bleach
Direct Reading
N/A
N/A
N/A
CaHR
CaHR Reagent Set
31073
CaHR-1
N/A
CaHR-2
N/A
CLLR
CLLR Reagent
31009
N/A
N/A
CLMR
CLMR Reagent
31004
N/A
N/A
CL2UH
CL2UH Reagent
31074
N/A
N/A
CLFTMB
CLFTMB Reagent
31075
N/A
N/A
Cyclo-F
Cyclo-F Reagent
31076
N/A
N/A
FeSSal
FeSSal Reagent
31078
N/A
N/A
NH3-F
Fluorescent
Method Set
31091
NH3-F-1
N/A
NH3-F-2
N/A
NH3-F-3
N/A
NO2D
Direct Reading
NA
N/A
N/A

Pyxis SP-800 Analyzer Procedures Manual 11
Method
Required Reagents
PN
Includes
PN
PAA
PAA Reagent
31079
N/A
N/A
POLY
POLY Reagent Set
31092
POLY-1
N/A
POLY-2
N/A
Sb3+
Sb3+ Reagent Set
31080
Sb3+ -1
N/A
Sb3+ -2
N/A
Sb3+ -3
N/A
Sb3+ -4
N/A
Sb-T
Sb-T Reagent Set
31080
Sb-T -1
N/A
Sb-T -2
N/A
Sb-T -3
N/A
Sb-T -4
N/A
SO3LR
SO3LR Reagent Set
31089
SO3LR-1
N/A
SO3LR-2
N/A
SO3LR-3
N/A
SO3HR
SO3HR Reagent Set
31090
SO3HR-1
N/A
SO3HR-2
N/A
SO3HR-3
N/A
Urea
Urea Reagent Set
31081
Urea-1
N/A
Urea-2
N/A
ZnXO
ZnXO Reagent Set
31052
ZnXO-1
N/A
ZnXO-2
N/A

Pyxis SP-800 Analyzer Procedures Manual 12
1. Turbidity
Test Program
Description：SP-800 Turbidity Method (1.0 - 200.0 NTU)
Instruments and Reagents:
1. SP-800 Portable Water Analyzer
2. 10-ml Sample Vial
Program:
1. Press OK key on the navigation control panel for 3 seconds until the screen lights
up. The main page will display eight major feature groups the main menu options.
Figure 1
2. Move the cursor to TURB icon using the navigational (left, right, up, or down) keys.
3. Fill the 10 ml sample vial with the test solution and tightly cap the sample vial.
Swirl the vial. Use a soft cloth or lint free paper tissue to clean the sample vial.
4. Place the sample vial into the sample vial compartment and slide the light shield
cover to the closed position.
Note: Mix the sample well before transferring it to the sample vial compartment.
5. Press the OK key in the main page. Pyxis SP-800 will start to measure the Turbidity
concentration in the sample.
6. Pyxis SP-800 will display the Turbidity concentration in NTU as turbidity.
2016/07/19 08:00
Power
M-F TURB
ABSB SYSCAL
COLOR
M-P

Pyxis SP-800 Analyzer Procedures Manual 13
Figure 2
Notes:
1. Collect samples in clean plastic or glass bottles. Analyze samples as soon as
possible. Store samples up to 48 hours by cooling to 4°C (39 °F). Analyze the
sample at the same temperature as it was collected.
2. If the sample contains air bubbles, tap the sample vial gently to remove the
bubbles before placing the sample vial to sample vial compartment.
Turbidity calibration
Deionized water (DI) as the blank calibration solution and the 50NTU /200NTU formazan
calibration standard solution are needed.
1. Press CAL on the main page, then choose the turbidity and press the OK key to
launch the turbidity calibration page.
2. Follow the message prompts, insert the DI blank into the sample vial
compartment and press the OK key to measure the deionized water.
Figure 3
3. Follow the message prompts and insert the 50 NTU formazan standard into the
sample vial compartment and press the OK key to measure the 50 NTU standard.

Pyxis SP-800 Analyzer Procedures Manual 14
Figure 4
4. Press the OK key to continue high range turbidity calibration. If high range
turbidity calibration not required, press any keys to exit.
Figure 5
5. Fill the 10 ml sample vial to above 10 ml mark with the 200 NTU formazan
standard. Insert the sample vial to the sample vial compartment.
6. Press the OK key to measure the 200 NTU standard. High range turbidity
calibration is successful.
Figure 6
7. Press any keys to exit.
8. If calibration fails, the followings should be checked:
•The DI blank is being contaminated.
•The 50 NTU/200NTU formazan standard solution is decayed or being
contaminated.
•The light shield cover is not in the closing position.

Pyxis SP-800 Analyzer Procedures Manual 15
•The sample vial compartment is blocked with debris, water, or other
materials.
Notes:
1. The center key is the OK key. Press the OK key on a selected item to launch the
action associated with the selected item.
2. Rinse all glassware with 1:1 hydrochloric acid solution. Rinse again with deionized
water.
3. When the sample vial is inserted into the sample vial compartment, the triangular
mark on the sample vial should be aligned approximately with the 6 o’clock
position of the sample vial compartment or any position consistently.
4. Pyxis SP-800 automatically turns itself off after 2 minutes with no-Key activity,
except for during a measurement. Pressing and holding the OK key for 3 seconds
will wake up the instrument, and return to the original page if it has any
measurement data.

Pyxis SP-800 Analyzer Procedures Manual 16
2. Aluminum –Al
Test Program
Description：SP-800 Aluminum Method (0.02 - 0.80 ppm Al) (Aluminon Method)
Instruments and Reagents:
1. SP-800 Portable Water Analyzer
210-ml Sample Vial
3. 25-ml Sample Vial
4. 50-ml Graduated Mixing Cylinder
5. Hach Aluminum Reagent (Cat. No.22420-00)
Includes:
(1) AluVer 3 Aluminum Reagent Powder Pillow (Cat. No.14290-99)
(2) Ascorbic Acid Powder Pillow (Cat. No.14577-99)
(3) Bleaching 3 Reagent Powder Pillow (Cat. No.14294-49)
Program:
1. Press OK key (the center key) on the navigation control panel for 3 seconds
until the screen lights up. The main page will display eight major feature
groups.
Figure 7
2. Position the cursor to COLOR icon by navigation keys and press the OK key to
enter COLOR selection interface, position the cursor to Al icon.

Pyxis SP-800 Analyzer Procedures Manual 17
Figure 8
3. Press the OK key to enter Al test program interface.
Figure 9
4. Fill a 50-ml graduated mixing cylinder to the 50-ml mark with sample.
Note: Rinse cylinder with 1:1 Hydrochloric Acid and deionized water before use
to avoid errors due to contaminants absorbed on the glass.
Note: Sample temperature must be 20-25 °C (68-77 °F) for accurate results.
5. Add the contents of one Ascorbic Acid Powder Pillow to the graduated mixing
cylinder. Swirl the vial to mix the reagent.
6. Add the contents of one AluVer® 3 Aluminum Reagent Powder Pillow to the
graduated mixing cylinder. Swirl the vial to mix the reagent.
Note: A red-orange color develops if aluminum is present.
Note: Inconsistent results will occur if any powder is undissolved.
7. Press the ZERO key.
8. Press the TMR1 Key to start the method timer, a 1-minute reaction period will
begin. Invert the cylinder repeatedly for three minutes.

Pyxis SP-800 Analyzer Procedures Manual 18
Figure 10
9. When the timer reaches the preset time and the reaction is complete, the
timer beeps.
10. Pour 10 ml of mixture in the cylinder into a 10-ml sample vial (the prepared
sample).
Note: There is 40 ml remaining solution in the graduated mixing cylinder.
11. Pour 25 ml of mixture in the cylinder into a 25-ml sample vial.
12. Add the contents of one Bleaching 3 Reagent Powder Pillow to 25-ml sample
vial. Stopper the cylinder.
Note: There is 15 ml remaining solution in the graduated mixing cylinder.
13. Press the TMR2 key to start the method timer, a thirty-second reaction period
will begin. vigorously shake the cylinder for the 30-second period.
Note: This solution should turn a light to medium orange upon bleaching. It will
not become colorless.
Figure 11
14. Press the TMR3 Key to start the method timer, a 15-minute reaction period will
begin.

Pyxis SP-800 Analyzer Procedures Manual 19
Figure 12
15. When the timer reaches the preset time and the reaction is complete, after the
timer beeps, the cursor will automatically switch to EXIT button. Move the
curse up to ZERO.
16. Pour 10 ml of mixture in the 25-ml sample vial into a 10-ml sample vial (the
blank sample).
17. Use a soft cloth or lint free paper tissue to clean the sample vial.
18. Place the prepared blank into the Pyxis SP-800 sample vial compartment and
press ZERO.
19. Place the prepared sample into the Pyxis SP-800 sample vial compartment and
press READ.
20. Concentration value based on the last absorbance value measured will be
calculated and displayed. Pyxis SP-800 will display the page.
Figure 13
21. Press EXIT to return to the main page.
The method is compatible with HACH 8012
Notes:
1. Rinse all glassware with 1:1 hydrochloric acid solution. Rinse again with
deionized water.
2. When the sample vial is inserted into the sample vial compartment, the
triangular mark on the sample vial should be aligned approximately with the 6
o’clock position of the sample vial compartment or any position consistently.

Pyxis SP-800 Analyzer Procedures Manual 20
3. Pyxis SP-800 automatically turns itself off after 2 minutes with no-Key activity,
except for during a measurement. Pressing and holding the OK Key for 3
seconds will wake up the instrument, and return to the original page if it has
any measurement data.
4. Press the CONF key in the method result page to launch the method setup and
calibration page. Press the FORM key to select a concentration form from the
list of forms (AL, AL2O3). Press the UNIT key to select a concentration unit
among the list of ppb, ppm, mg/L, and ug/L.