Pyxis SP-380P User manual

SP-380P Handheld Dual Fluorometer
Operation Manual
August. 24, 2020
Rev. A
Pyxis Lab, Inc.
1729 Majestic Dr. Suite 5
Lafayette, CO 80026 USA
www.pyxis-lab.com
© 2020 Pyxis Lab, Inc.
Pyxis Lab Proprietary and Confidential

Contents
1General Description.........................................................................................................1
1.1 Specification.............................................................................................................1
1.2 Pyxis SP-380P Major Features..................................................................................2
1.3 Unpacking the Instrument .......................................................................................2
1.4 Standard Accessories ...............................................................................................2
1.5 Optional Accessories ................................................................................................3
1.6 Structure Description ...............................................................................................4
2Start Pyxis SP-380P..........................................................................................................4
2.1 Battery Installation...................................................................................................4
2.2 Description of the Control Keys ...............................................................................6
2.3 Turning On/Off Pyxis SP-380P..................................................................................6
3Fluorescent Polymer and PTSA Measurement................................................................7
3.1 Measurement...........................................................................................................7
3.2 High Color and Turbidity Warning............................................................................7
4Calibration .......................................................................................................................7
4.1 PTSA Calibration.......................................................................................................7
4.2 Fluorescent polymer Calibration............................................................................11
4.3 Quick Tips...............................................................................................................14
5Device Information and Diagnosis.................................................................................14
6Bluetooth Connection ...................................................................................................15
7SP-380P Cleaning...........................................................................................................16
7.1 Cleanliness Check...................................................................................................16
7.2 Cleaning Procedure ................................................................................................17
8REGULATORY APPROVAL...............................................................................................18
9Contact us......................................................................................................................18
10 Appendix. Cleaning Kit...................................................................................................19

- 2 -
Confidentiality
The information contained in this manual may be confidential and proprietary and is the property of Pyxis Lab
Inc. Information disclosed herein shall not be used to manufacture, construct, or otherwise reproduce the
goods disclosed herein. The information disclosed herein shall not be disclosed to others or made public in any
manner without the express written consent of Pyxis Lab Inc.
Standard Limited Warranty
Pyxis Lab warrants its products for defects in materials and workmanship. Pyxis Lab will, at its option, repair or
replace instrument components that prove to be defective with new or remanufactured components (i.e.,
equivalent to new). The warranty set forth is exclusive and no other warranty, whether written or oral, is
expressed or implied.
Warranty Term
The Pyxis warranty term is thirteen (13) months ex-works. In no event shall the standard limited warranty
coverage extend beyond thirteen (13) months from original shipment date.
Warranty Service
Damaged or dysfunctional instruments may be returned to Pyxis for repair or replacement. In some instances,
replacement instruments may be available for short duration loan or lease.
Pyxis warrants that any labor services provided shall conform to the reasonable standards of technical
competency and performance effective at the time of delivery. All service interventions are to be reviewed and
authorized as correct and complete at the completion of the service by a customer representative, or
designate. Pyxis warrants these services for 30 days after the authorization and will correct any qualifying
deficiency in labor provided that the labor service deficiency is exactly related to the originating event. No
other remedy, other than the provision of labor services, may be applicable.
Repair components (parts and materials), but not consumables, provided in the course of a repair, or
purchased individually, are warranted for 90 days ex-works for materials and workmanship. In no event will the
incorporation of a warranted repair component into an instrument extend the whole instrument’s warranty
beyond its original term.
Shipping
A Repair Authorization Number (RA) must be obtained from the Technical Support ([e-mail])
before any product can be returned to the factory. Pyxis will pay freight charges to ship replacement or
repaired products to the customer. The customer shall pay freight charges for returning products to Pyxis. Any
product returned to the factory without an RA number will be returned to the customer.

1
1General Description
1.1 Specification
Item
Specification
P/N
50402
Fluorescent Polymer*
0.0-20.0 ppm
PTSA
0.0-300.0 ppb
Excitation Wavelength
410 nm (Fluorescent Polymer)
365 nm (PTSA)
Emission Wavelength
470 nm (Fluorescent Polymer)
410 nm (PTSA)
Wavelength Accuracy
±1 nm
Precision
± 0.1 ppm (3 Sigma, Fluorescent Polymer)
± 0.5 ppb (3 Sigma, PTSA)
Accuracy
±1% or ±0.1 ppm (Fluorescent Polymer)
±1% or ±1 ppb (PTSA)
Calibration Solution Point
0, 10, 20 ppm (Fluorescent Polymer)
0,100,200 ppb (PTSA)
Battery
4 AA alkaline batteries for main module
Typical Battery Life
3200 readings (480mAh battery)
Display
320x240 TFT-LCD, visible under direct sunlight
Dimension
L170.W80.H45 (mm)
Weight
400g (without battery)
Temperature Range
40 to 106 oF (4 to 41 oC)
Humidity
85% at 106 oF (41 oC)
Environmental
IP67, dustproof and waterproof
Regulation
CE / RoHS
*The fluorescent polymer concentration scale is based on the polymer containing 0.25 mole %
fluorescent monomer.

2
1.2 Pyxis SP-380P Major Features
The Pyxis SP-380P analyzer measures the concentration of fluorescent polymer or PTSA of
a water sample. Main features include:
•The SP-380P is pre-calibrated for measuring fluorescent polymer in the range of 0
to 20 ppm or PTSA in the range of 0 to 500 ppb. The fluorescent polymer or PTSA
measurement is automatically compensated for sample color and turbidity
interference.
•Fluorescent polymer can be calibrated with using three standards (0,10, and 20
ppm) through a user-friendly menu-driven procedure. Similarly, PTSA can also use
three calibrators (0,100 and 200 ppb) for calibration.
•No sample cuvette is required and as such variations associated with the cuvette
are eliminated.
•Large color graphic screen that can be read in direct sunlight.
1.3 Unpacking the Instrument
Remove the instrument and accessories from the shipping container and inspect each item
for any damage that may have occurred during shipping. Verify that all items listed on the
packing slip are included. If any items are missing or damaged, please contact Pyxis
Customer Service at [email protected]om.
1.4 Standard Accessories
•Quick-Start Instruction Guide
•4 AA alkaline batteries
•Bluetooth USB adapter (P/N: MA-NEB)
•Full instrument manual is available from https://pyxis-lab.com/support-2/
MA-NEB USB Adapter

3
1.5 Optional Accessories
Item
P/N
Specification
Pyxis Handheld
Cleaning Kit
SER-02
Pyxis Handheld Sample Well
Cleaning Solution Kit –500 mL
PTAG-1010
21055
Fluorescent Polymer10ppm
PTSA 100ppb
Combination Cal. Std
PTSA-100
21001
PTSA 100 ppb
Calibration Std –500 mL
PTSA-200
21031
PTSA 200 ppb
Calibration Std –500mL
TAG-10
21054
Fluorescent Polymer 10 ppm
Calibration Std –500 mL
TAG-20
21053
Fluorescent Polymer 20 ppm
Calibration Std –500 mL
MA-700
50725
Pyxis Handheld Hard Carry Case

4
1.6 Structure Description
The structure description is shown in Figure 1.
Figure 1: SP-380P Structure Description
2Start Pyxis SP-380P
2.1 Battery Installation
The SP-380P is powered by 4 AA alkaline batteries. Do not use rechargeable nickel cadmium
(NiCad) or lithium batteries. The 4 AA alkaline batteries will last sufficient for about 3000
measurements. When the battery capacity is critically low, the SP-380P will display a LOW
BATTERY warning for 5 seconds and then automatically turn off.

5
Replace the battery to resume operation of the SP-380P after the battery warning. The SP-
380P will automatically turn on in the measurement mode after new battery installation.
The SP-380P battery compartment, shown in Figure 2 is on the back side of the instrument.
Install battery as follows:
•Remove the battery compartment cover by loosening two screws.
•Make sure that snap the battery firmly into the battery holder.
•Replace the battery compartment cover, making sure that the sealing O-ring is lying flat
on the battery compartment. To prevent the SP-380P from accidently being turned on
due to vibration, please firmly tighten the two screws. The 4 AA alkaline batteries will
last for about 3000 measurements. When the battery capacity is critically low, the SP-
380P will display a LOW BATTERY warning for 5 seconds and then automatically turn off.
Figure 2: Battery Installation

6
2.2 Description of the Control Keys
The SP-380P has three keys as shown in Figure 3 The left (<), right (>) and OK keys are used
to launch an action indicated on the screen directly above the keys. Please note that the
screen is not a touch screen. The labels above the keys indicate the function associated with
the keys and can change according to the screen modes.
Figure 3: Keys & Associated Functions
2.3 Turning On/Off Pyxis SP-380P
To turn on the SP-380P, press OK momentarily and release.
To turn off the SP-380P, press and hold the OK key. Release the OK key when the LCD display
turns off (after about 3 seconds). The SP-380P will turn itself off after 60 seconds without
user interaction through the keys.

7
3Fluorescent Polymer and PTSA Measurement
3.1 Measurement
When powered on, the SP-380P will be in the measurement (read) mode (see Figure 3). The
displayed value is the concentration of PTSA and Fluorescent Polymer measurement.
The water sample can be transferred to the measurement cell using a pipette or filled
directly from a faucet, sample bottle, or sample valve. The SP-380P does not need to be
turned off between measurements of two samples. Rinsing the measurement cell several
times is recommended.
For a sample containing 10 ppm fluorescent polymer, the measured fluorescent polymer
should be stabilized within the range of 9.8 to 10.2 ppm. Similarly, for samples containing
100 ppb PTSA, the measured PTSA should be stable in the range of 99.5 to 100.5 ppb.
3.2 High Color and Turbidity Warning
The SP-380P has extra channels to measure sample turbidity and color which are used to
automatically compensate for any interference caused by these contaminants as high as 40-
60NTU. If the sample turbidity and color values determined are too high, a measurement
warning will be displayed. In such a case, the user should pre-filter the sample for a
fluorescent polymer or PTSA measurement within the compensation capability of the SP-
380P.
4Calibration
4.1 PTSA Calibration
The SP-380P PTSA measurement can be calibrated. To calibrate PTSA measurement
requires the 100, or 200 ppb PTSA standard solution from Pyxis Lab.
PTSA Calibration (2 Point with Zero)
1) Rinse sample cell with DI water. Fill sample cell with DI water.
*NOTE* In an emergency, “non-PTSA”water, such as city water, may be used, but re-
calibrate using DI water for the zero step as soon as it is available.
2) Power on by a short press of OK key. Allow 5-10 seconds for meter to stabilize.

8
3) A screen similar to Figure 3 will appear. The unit is actively reading and displaying the
PTSA and fluorescent polymer residual in the sample. The values will be very low if DI
water is used; PTSA and fluorescent polymer value should be near zero. A low non-
zero value (e.g. 0.1 or 0.2, etc.) is normal and not problematic.
4) Press P-Cal labeled key (＜),see Figure 3.
5) Figure 4 the first screen of the PTSA (alone) calibration will appear.
Figure 4
6) Insert DI Solution, and press Zero labeled key (<) to set the zero point.
7) After a successful zero calibration a checkmark symbol will appear next to “Press
Zero Button” to confirm success. The screen will also update to show the Slope steps,
as in Figure 5. The Zero on the black bar is replaced with Cycle command and the
possible PTSA selection is displayed in red. The default is 100 ppb.

9
Figure 5
8) Rinse the sample cell out thoroughly (twice at least) with the desired PTSA standard
and ensuring the measurement sample cell is nearly full.
9) If the 100 ppb PTSA default is not the desired PTSA for calibration, press the Cycle
labeled key (<) to switch between the PTSA standards 100 or 200 ppb (press
repeatedly). The value in red will update as the setting is changed. If the default of
100 ppb is desired then the use of Cycle (<) is not required. Ensure the value selected
matches the standard actually utilized.
10) Press the Slope labeled key (>) to set the slope of the standard desired and complete
PTSA calibration.
11) If calibration is successful, the screen will update with a second checkmark for the
Slope setting as in Figure 6 and the message Calibration Succeed will appear.

10
Figure 6
12) Press the Exit labeled key (OK) to return to the measurement screen. The screen will be
similar to Figure 7. Slight variance in the PTSA value is not problematic. If Exit button is
pushed before the second checkmark appears, the calibration will not be completed and
must be re-done.
Figure 7

11
4.2 Fluorescent polymer Calibration
The SP-380P fluorescent polymer measurement can be calibrated. To calibrate fluorescent
polymer measurement requires the 10 or 20 ppm fluorescent polymer standard solution
from Pyxis Lab.
Fluorescent polymer Calibration (2 Point with Zero)
1) Rinse sample cell with DI water. Fill sample cell with DI water.
*NOTE* In an emergency, “non-fluorescent polymer” water, such as city water, may be
used, but re-calibrate using DI water for the zero step as soon as it is available.
2) Power on by a short press of OK key. Allow 5-10 seconds for meter to stabilize.
3) A screen similar to Figure 3 will appear. The unit is actively reading and displaying the
PTSA and fluorescent polymer residual in the sample. The values will be very low if DI
water is used; PTSA and fluorescent polymer value should be near zero. A low non-zero
value (e.g. 0.1 or 0.2, etc.) is normal and not problematic.
4) Press F-Cal labeled key (>).
5) Figure 8, the first screen of the Fluorescent Polymer (alone) calibration will appear.
Figure 8

12
6) Insert DI Solution, and press Zero labeled key (<) to set the zero point.
7) After a successful zero calibration a checkmark symbol will appear next to “Press
Zero Button” to confirm success. The screen will also update to show the Slope steps,
as in Figure 9. The Zero on the black bar is replaced with Cycle command and the
possible fluorescent polymer selection is displayed in red. The default is 10 ppm.
Figure 9
8) Rinse the sample cell out thoroughly (twice at least) with the desired fluorescent
polymer standard and ensuring the measurement sample cell is nearly full.
9) If the 10 ppm fluorescent polymer default is not the desired fluorescent polymer for
calibration, press the Cycle labeled key (<) to switch between the fluorescent
polymer standards 10 or 20 ppm (press cycle repeats). The value in red will update
as the setting is changed. If the default of 10 ppm is desired then the use of Cycle
(<) is not required. Ensure the value selected matches the standard actually utilized.
10) Press the Slope labeled key (>) to set the slope of the standard desired and complete
fluorescent polymer calibration.
11) If calibration is successful, the screen will update with a second checkmark for the
Slope setting as in Figure 10 and the message Calibration Succeed will appear.

13
Figure 10
12) Press the Exit labeled key (OK) to return to the measurement screen. The screen will be
similar to Figure 11. Slight variance in the fluorescent polymer value is not problematic.
If Exit is done before the second checkmark appears, the calibration will not be
completed and must be re-done.
Figure 11

14
4.3 Quick Tips
1) If the 100 ppb PTSA concentration (the default) is the desired calibration, then the
key presses from the beginning, including the power on, are: OK, < , <, {refill with
PTSA standard}, >, then after completion, Exit to return to Measurement Mode.
Similarly, if 10 ppm fluorescent polymer is the desired calibration value, press the
following keys from the beginning to quickly complete the calibration: OK, >, <,
{refill fluorescent polymer standard},>, then after completion, Exit to return to
Measurement Mode.
2) Using the PTSA as an example, if screen darkens, the timer will shortly power down
the meter. Any key press will reset the timer, but this press does not perform any
activity other than timer restart. The next key press needed must still be done after
this timer re-set press. The timer is set to help maximize battery life. After the key
press to set the zero point, there is 40 seconds to rinse and refill the measurement
cell with PTSA standard before the next key press of either “Cycle” (<) to change
PTSA setting or “Slope” (>) to execute the final part of the calibration.
3) After returning to read mode after calibration, rinse several times with the first
sample. The unit will continue to read the sample values without any further key
presses if it has not powered off. If there are no key presses for 20 seconds the
screen will darken (40 seconds in a calibration mode), and after another 20 seconds
without key activity the unit will power down. If you have multiple samples a quick
press on OK or the other keys will keep the timer going, giving you time to add the
next one. To ensure accurate results and avoid sample carry-over/contamination,
rinse at least twice with the next sample before proceeding.
4) Always rinse the unit with clean water after each use and dry by clean tissue or paper
towel.
5) After a successful calibration, the unit does not automatically return to the
measurement mode. If Exit is held down too long the unit will power down rather
than returning to the measurement mode.
5Device Information and Diagnosis
The device information is shown when the Info labeled OK key in the measurement mode is
pressed momentarily. The screen contains the device serial number, software version, and
hardware version (Figure 12). The battery life as a percentage and the standard that were
used in the last calibration are also shown.

15
Press the diagnosis labeled key to switch to the diagnosis screen where raw measurement
data are displayed (Figure 13). The information has no use for normal operation. Please
provide an image of both the device information screen and the diagnosis screen when you
contact Pyxis ([email protected]) for troubleshooting your device.
Figure 12
Figure 13
6Bluetooth Connection
The Pyxis SP-380P can be connected to a smart phone/device or a
computer via the built-in Bluetooth Low Energy Connection (BTLE).
The uPyxis© app for smartphone is available for free download to
both Apple and Samsung devices per QR codes below.
A laptop with uPyxis© app can use the Bluetooth Adapter (MA-NEB)
included with the SP-380P as a standard accessory to connect to the
SP-380P for parameter configuration, firmware upgrade, and other
tasks. The Pyxis uPyxis software can be downloaded from
www.pyxis-lab.com/support.html
The SP-380P can be paired with other Pyxis devices for exchanging
data over Bluetooth. In the normal operation modes, the Bluetooth
function is turned off. To turn on the Bluetooth wireless function,
press Info, and then press Diagnosis. Other SP-380P wireless
functions are available. For more information, contact Pyxis Lab
([e-mail]) or call at 1-866-203-8397.
Bluetooth Adapter for PC (MA-NEB)

16
7SP-380P Cleaning
The SP-380P is designed to provide reliable and accurate measurement on fluorescent
polymer and PTSA. Heavy fouling will prevent the light from reaching the sensor, resulting
in inaccurate readings. It is suggested that the SP-380P be checked for fouling and cleaned
on a monthly basis. Heavily contaminated waters may require more frequent cleanings.
Cleaner water sources with less contamination may not require cleaning for several months.
7.1 Cleanliness Check
SP-380P is designed to do the Cleanliness Check as described below:
1. Press Info (the OK key) to launch Device Information page (as shown in Figure 12)
2. Press Diagnosis (the <key) to switch to System Diagnosis page (as shown in Figure 13)
3. Press Cleanliness Check (the <key). An instruction prompt appears to ask USER to put
DI water into the measurement cell (as shown in Figure 14)
4. Put DI water into the measurement cell
5. Press Confirm (the <, >or OK key). The instruction prompt will disappear and SP-380P
begins to check if fouling.
6. Checking result will be displayed in Diagnosis page as shown in Figure 15 and Figure
16.
Figure 14
Figure 15
Figure 16