Pyxis SP-200 OXIPOCKET User manual

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SP-200 Handheld Colorimetric Analyzer
User Manual
February 8, 2021
Rev. 1.02
Pyxis Lab, Inc.
1729 Majesc Dr. Suite 5
Lafayee, CO 80026 USA
www.pyxis-lab.com
© 2017 Pyxis Lab, Inc.
Pyxis Lab Proprietary and Condenal

Table of Contents
1 Introducon 3
1.1 Main Features . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3
2 Specicaons 3
3 Unpacking Instrument 3
3.1 Standard Accessories . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4
3.2 Oponal Accessories . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4
4 Installaon 5
4.1 Baery Installaon . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5
5 Instrument Overview 6
5.1 Control Keys . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6
5.2 Main Module On/O . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6
6 Measurement 7
6.1 Colorimetric Methods . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7
6.2 Select a Method . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7
6.3 Single-Vial Measurement . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8
7 Calibraon 9
7.1 Set Method Parameters . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9
7.2 Low Calibraon . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 10
7.3 Slope Calibraon . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11
7.4 Restore Factory Default Values . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 12
8 Device Informaon and Diagnosis 13
8.1 Bluetooth Connecon to Devices . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 13
8.2 Factory Reset . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 14
9 Use with uPyxis® Mobile App 15
9.1 Download uPyxis® Mobile App . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 15
9.2 Connecng to uPyxis® Mobile App . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 16
9.3 Seng Screen . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 17
9.4 System Screen . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 18
9.5 Datalog Screen . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 19
10 Use with uPyxis® Desktop App 19
10.1 Install uPyxis® Desktop App . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 19
10.2 Connecng to uPyxis® Desktop App . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 20
10.3 System Screen . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 21
10.4 Seng Screen . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 22
10.5 Datalog Screen . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 22

11 Device Maintenance and Precauon 23
11.1 Maintenance Best Pracces and Quick Tips . . . . . . . . . . . . . . . . . . . . . . . . . . . . 23
11.2 Storage . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 23
12 Contact Us 23
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Warranty Informaon
Condenality
The informaon contained in this manual may be condenal and proprietary and is the property of Pyxis
Lab, Inc. Informaon disclosed herein shall not be used to manufacture, construct, or otherwise reproduce
the goods described. Informaon disclosed herein shall not be disclosed to others or made public in any
manner without the express wrien consent of Pyxis Lab, Inc.
Standard Limited Warranty
Pyxis Lab warrants its products for defects in materials and workmanship. Pyxis Lab will, at its opon, repair
or replace instrument components that prove to be defecve with new or remanufactured components
(i.e., equivalent to new). The warranty set forth is exclusive and no other warranty, whether wrien or oral,
is expressed or implied.
Warranty Term
The Pyxis warranty term is thirteen (13) months ex-works. In no event shall the standard limited warranty
coverage extend beyond thirteen (13) months from original shipment date.
Warranty Service
Damaged or dysfunconal instruments may be returned to Pyxis for repair or replacement. In some in-
stances, replacement instruments may be available for short duraon loan or lease.
Pyxis warrants that any labor services provided shall conform to the reasonable standards of technical com-
petency and performance eecve at the me of delivery. All service intervenons are to be reviewed and
authorized as correct and complete at the compleon of the service by a customer representave, or des-
ignate. Pyxis warrants these services for 30 days aer the authorizaon and will correct any qualifying
deciency in labor provided that the labor service deciency is exactly related to the originang event. No
other remedy, other than the provision of labor services, may be applicable.
Repair components (parts and materials), but not consumables, provided during a repair, or purchased
individually, are warranted for 90 days ex-works for materials and workmanship. In no event will the in-
corporaon of a warranted repair component into an instrument extend the whole instrument’s warranty
beyond its original term.
Warranty Shipping
A Repair Authorizaon (RA) Number must be obtained from Pyxis Technical Support before any product can
be returned to the factory. Pyxis will pay freight charges to ship replacement or repaired products to the
customer. The customer shall pay freight charges for returning products to Pyxis. Any product returned to
the factory without an RA number will be returned to the customer. To receive an RMA you can generate a
request on our website at hps://pyxis-lab.com/request-tech-support/.
Pyxis Technical Support
Contact Pyxis Technical Support at [phone], [email protected], or by lling out a request for
support at hps://pyxis-lab.com/request-tech-support/.
SP-200 User Manual [email protected] | [phone]

1 Introducon
The Pyxis SP-200 Handheld Colorimetric Analyzer is a single- and mul-parameter colorimetric tesng plat-
form. With the use of auxiliary colorimetric reagent kits, the SP-200 can quickly test a variety of water
quality parameters on the spot with high sensivity.
1.1 Main Features
The SP-200 includes the following features:
• Innovave technology plaorm with colorimetric measurement of 6 LED wavelengths
• Use of matching reagent kits and prefabricated reagent tubes
• Long standby mainframe 10,000+ measurements
• Can store up to 8 GB of test data (including test me)
• Create user-dened standard curves, view historical data, or analyze data via the uPyxis® App.
2 Specicaons
Table 1. SP-200 Specicaons
Item Specicaon*
Part Number (P/N) 50802
Absorbance Linearity Range 0–2.0 Abs
Absorbance Reproducibility ±0.005 Abs (0–1.0 Abs)
Colorimeter Wavelength 365/420/470/525/568/624 nm
Wavelength Accuracy ±1 nm
Temperature Range 40–106 °F (4–41 °C)
Humidity 85% at 106 °F (41 °C)
Data Storage 30,000 group data storage, Bluetooth transmission
Display 320x240 TFT-LCD, visible under direct sunlight
Power Supply 4 AA alkaline baeries
Typical Baery Life 10,000 readings
Cuvee 24mm diameter cuvee
Dimension (L ×W×H) 6.69 ×3.15 ×1.77 inch (170 ×80 ×45 mm)
Weight †0.88 lbs (400 g)
Enclosure Rang IP67
Regulaon CE/RoHS
*With Pyxis’s connuous improvement policy, these specicaons are subject to change with-
out noce.
†Baeries excluded
3 Unpacking Instrument
Remove the instrument and accessories from the shipping container and inspect each item for any damage
that may have occurred during shipment. Verify that all items listed on the packing slip are included. If any
items are missing or damaged, please contact Pyxis Customer Service at [email protected]om.
SP-200 User Manual [email protected] | [phone]

3.1 Standard Accessories
• Quick-Start Guide
• Four (4) AA alkaline baeries
• Two (2) 10 mL Sample Vials, 24 mm diameter P/N: MA-24
• Bluetooth/USB Adapter for Desktop P/N: MA-NEB
• User Manual available online at hps://pyxis-lab.com/support/
3.2 Oponal Accessories
The following oponal accessories can be ordered from Pyxis Customer Service ([email protected]om) or
Pyxis E-Store at hps://pyxis-lab.com/shop/.
Table 2. Oponal Accessories
Accessory Name Part Number (P/N)
Pyxis Carrying Case for Handheld Devices 50725
10 mL Sample Vial MA-24
25 mL Sample Vial MA-25
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4 Installaon
4.1 Baery Installaon
The SP-200 is powered by four alkaline baeries. Typical baery life lasts for 10,000 measurements or 10
months. When the baery capacity is crically low, the SP-200 will display a ”LOW BATTERY” warning for 5
seconds and then automacally turn o.
*NOTE*Do not use rechargeable nickel cadmium (NiCad) or lithium baeries.
Replace the baeries to resume operaon of the SP-200 aer the baery warning. The SP-200 will auto-
macally turn on in the measurement mode aer new baeries are installaon.
The SP-200 baery compartment, shown in Figure ??, is on the back side of the instrument. Baeries are
held in place by a cover secured with two Phillips-head screws.
Figure 1. The SP-200 baery compartment
Install baeries using the following steps:
1. Remove the baery compartment cover by loosening the two screws.
2. Remove old baeries and dispose of them properly.
3. Following the posive and negave terminal signs in the compartment boom, snap four new AA
alkaline baeries rmly into the baery holder.
4. Replace the baery compartment cover and ensure that the sealing O-ring is lying at on the baery
holder.
5. Fasten the two screws.
*NOTE*Failure to properly seat the O-ring may result in water damage to the SP-200.
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5 Instrument Overview
Figure 2.
5.1 Control Keys
The SP-200 has three control keys, as shown in Figure 2. The le ( ), right ( ), and ok ( ) keys
are used to launch acons indicated on the LCD display directly above the keys. The labels above the keys
indicate the funcon associated with each key and funcons can be changed in dierent operaon modes.
5.2 Main Module On/O
To turn on the SP-200: Press momentarily and release.
To turn o the SP-200: Press and hold for about three seconds. Release when the LCD display
turns o. The SP-200 turns itself o aer 60 seconds without user interacon detected. This is done to
conserve baery life.
*NOTE*This auto-me o seng may be customized by the user as desired through the
uPyxis® Mobile or Desktop App.
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6 Measurement
6.1 Colorimetric Methods
A wide range of colorimetric methods is supported by the SP-200 analyzer and the number of them keeps
increasing with connuous development of Pyxis. The following table is list of Supported Colorimetric Meth-
ods:
Table 3. Supported Colorimetric Methods
Abbreviaon Chemical Measured Method Type Range
CL-F Free Chlorine DPD 2.2 ppm
CL-T Total Chlorine DPD 2.2 ppm
Br-T Total Bromine DPD 4.5 ppm
CLO2 Chlorine Dioxide DPD 5.0 ppm
PAA Peroxyacec Iodometry 500 ppm
H2O2 Hydrogen Peroxide Iodometry 500 ppm
Bleach-L Bleach — Low Range Direct Read 1.0%
Bleach-H Bleach — High Range Direct Read 16.0%
O3 Ozone DPD 1.0 ppm
NH2C Chloramine, Mono — Low Range Indophenol 3.0 ppm
CLO2D Chlorine Dioxide — Mid Range Direct Read 50 ppm
CLO2H Chlorine Dioxide — High Range Direct Read 1500 ppm
CL2H Free/Total Chlorine — High Range DPD 10 ppm
CL2UH Free/Total Chlorine — Ultra High Range Iodometry 400 ppm
6.2 Select a Method
When powered on, the SP-200 is in the screen of the last selected measurement method, as shown in the
Figure 3. Use the following steps to select a dierent method:
1. Press Color ( ) to launch the Method screen (Figure 4).
*NOTE*The top line of the page shows the most frequently selected method, marked
by the ☆.
2. Press Up () and Down ( ) to navigate to the desired method.
*NOTE*Long press to exit without changing the method.
3. Press to select and start the highlighted method.
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Figure 3. Figure 4.
6.3 Single-Vial Measurement
1. Make the Blank sample by lling a sample vial with the water sample and place it in the sample vial
compartment of the SP-200.
2. Press Zero (). Zero will then appear on the top-le corner of the display (Figure 5).
3. Add the appropriate reagent to the Blank sample.
4. Make the Developed sample by capping the sample vial and inverng several mes to fully mix the
reagent and sample.
5. Place the Developed sample in the sample vial compartment SP-200.
6. Press TMR1 ( ) to start the method mer (Figure 6).
7. The SP-200 will connuously display the concentraon as the mer counts down. Record the nal
value aer mer stops (Figure 7).
*NOTE*The rate of the reacon is oen faster than the standard preset me. If
the concentraon remains stable on the concentraon-me plot, press Stop (
) to terminate the ming step early. The last concentraon measurement will be
displayed.
Figure 5. Figure 6. Figure 7.
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7 Calibraon
7.1 Set Method Parameters
Each method has two parameters that can be changed by the user; the units and the chemical formula
form. To change these parameters for a given method, use the following steps:
1. Complete a measurement with the desired method.
2. Press Cal/Sys ( ) as needed to highlight either Unit or Form.
3. Press OK () to launch the Choose Unit screen (Figure 8) or the Choose Form screen (Figure 9).
4. Press »() to navigate to the desired parameter opon.
5. Press OK () to select the desired parameter opon.
Figure 8. Figure 9.
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7.2 Low Calibraon
Some methods have a non-zero intercept value in the calibraon equaon. For these methods, the appro-
priate non-zero intercept value is shipped pre-loaded in the SP-200. The following steps are used to carry
out a low calibraon which changes this non-zero intercept value:
1. Complete a measurement with the desired method.
2. Press Cal/Sys () as needed to highlight Low-C.
3. Press OK () to launch the calibraon screen (Figure 10).
4. Fill a sample vial with deionized (DI) water or with soluon with a known concentraon and place it
in the sample vial compartment of the SP-200.
*NOTE*For best results, the know concentraon should be less than half of the max-
imum concentraon of the method. See the Supported Colorimetric Methods table
for concentraon ranges for each method.
5. Use «() and -( ) to adjust the concentraon value to match the known concentraon of the
soluon or to zero if using deionized (DI) water.
6. Press Calib () to connue with the entered concentraon value. The display updates as shown
in Figure 11.
7. Press OK () to start the low calibraon.
8. The message “Low point calibraon succeeded” will appear on the display (Figure 12).
9. Press to exit.
Figure 10. Figure 11. Figure 12.
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7.3 Slope Calibraon
Each method has been calibrated prior to shipping, there is no need to calibrate unless a calibraon check
indicates that the method needs a calibraon. The following steps are used to carry out a slope calibraon:
1. Complete a measurement with the desired method.
2. Press Cal/Sys () as needed to highlight Slope-C.
3. Press OK () to launch the calibraon screen (Figure 13).
4. Fill a sample vial with a soluon with known concentraon and place it in the sample vial compart-
ment of the SP-200.
*NOTE*For best results, the know concentraon should be less maximum concentra-
on of the method. See the Supported Colorimetric Methods table for concentraon
ranges for each method.
5. Use «() and -( ) to adjust the concentraon value to match the known concentraon of the
soluon.
6. Press Calib () to connue with the entered concentraon value. The display updates as shown
in Figure 14.
7. Press OK () to start the slope calibraon.
8. The message “High point calibraon succeeded” will appear on the display (Figure 15).
9. Press to exit.
Figure 13. Figure 14. Figure 15.
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7.4 Restore Factory Default Values
Each method can be restored to the factory default intercept and slope values, individually. The following
steps are used to carry out a factory default of the desired method’s intercept and slope values:
1. Complete a measurement with the desired method.
2. Press Cal/Sys () as needed to highlight Default.
3. Press OK (). The display updates as shown in Figure 16.
4. Press OK () to start the factory default of the desired method.
5. The message “Restore data succeeded” will appear on the display (Figure 17).
Figure 16. Figure 17.
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8 Device Informaon and Diagnosis
The Informaon screen is launched when Cal/Sys ( ) is pressed while not in an ongoing measurement
procedure. This screen contains the device serial number, soware version, and hardware version (Figure
18). The baery life as a percentage and the MAC addresses for main module also shown.
Press Diag () to launch the Diagnosis screen where raw measurement data are displayed (Figure 19).
The informaon has no use for normal operaon, but instead is used for device troubleshoong. Provide
an image of both the Informaon screen and the Diagnosis screen when you contact Pyxis (service@pyxis-
lab.com) for troubleshoong your device or call [phone].
Figure 18. Figure 19.
8.1 Bluetooth Connecon to Devices
The SP-200 uses a built-in Bluetooth Low Energy Connecon (BTLE) to connect wirelessly to a smart phone
via the uPyxis® Mobile App or to a computer via the included Bluetooth Adapter (P/N: MA-NEB) and the
uPyxis® Desktop App. To allow the SP-200 to connect via Bluetooth with other devices follow the steps
below:
1. Power on the SP-200 by pressing .
2. Press Cal/Sys () to launch the Informaon screen.
3. Press Diag () to launch the Diagnosis screen.
4. Allow 5–10 seconds for the message in the top-right corner of the display change from Starng BTLE...
to BTLE Started (Figure 19).
5. Choose to connect via one of two opons:
(a) The uPyxis® Mobile App (see the Use with uPyxis® Mobile App secon), or
(b) The uPyxis® Desktop App (see the Use with uPyxis® Desktop App secon).
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8.2 Factory Reset
Use the following steps to restore all device parameters to factory default:
1. Power on the SP-200 by pressing .
2. Press Cal/Sys () to launch the Informaon screen.
3. Press Diag () to launch the Diagnosis screen.
4. Allow 5–10 seconds for the message in the top-right corner of the display change from Starng BTLE...
to BTLE Started .
5. Press Factory (). The display updates as shown in Figure 20.
6. Press OK () to start the factory reset.
7. The message “Data recovery is successful!” will appear on the display (Figure 21).
Figure 20. Figure 21.
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9 Use with uPyxis® Mobile App
9.1 Download uPyxis® Mobile App
Download uPyxis® Mobile App from Apple App Store or Google Play.
Figure 22. uPyxis® Mobile App installaon
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9.2 Connecng to uPyxis® Mobile App
Connect the SP-200 sensor to a mobile smart phone according to the following steps:
1. Follow the steps in the Bluetooth Connecon to Devices secon to make the SP-200 discoverable.
2. Open uPyxis® Mobile App.
3. On uPyxis® Mobile App, pull down to refresh the list of available Pyxis devices.
4. If the connecon is successful, the SP-200 and its Serial Number (SN) will be displayed (Figure 26).
5. Press on the SP-200 image.
Figure 23.
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9.3 Seng Screen
From the Seng screen, the user can set the Power o me and Screen o me in seconds.
Figure 24.
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