Pyxis SP-910 User manual

SP-910 Portable Water Analyzer
Operation Manual
Rev.B
©Pyxis Lab, Inc 2022
All Rights Reserved
Pyxis Lab, Inc
1729 Majestic Dr Suite 5
Lafayette, CO 80026 USA
www.pyxis-lab.com
[e-mail]

i
Contents
1 General Description ................................................................................................................................... 1
1.1 Specification........................................................................................................................................ 1
1.2 Pyxis Major Features........................................................................................................................... 2
1.3 Unpackaging the Instrument .............................................................................................................. 2
1.4 Standard Accessories .......................................................................................................................... 3
1.5 Optional Accessories........................................................................................................................... 3
1.6 Sample Vial Compartment.................................................................................................................. 3
1.7 Light Shield Cover................................................................................................................................ 3
2 Start the SP-910 ......................................................................................................................................... 5
2.1. Battery Installation............................................................................................................................. 5
2.1 Description of the Navigational Control Pad ...................................................................................... 5
2.2 Turning on the SP-910......................................................................................................................... 5
2.3 Main Page ........................................................................................................................................... 6
2.4 Turning off the SP-910 ........................................................................................................................ 6
2.5 The SP-910 Auto Power off................................................................................................................. 6
2.6 Auto LCD Power Saving....................................................................................................................... 7
3 PTSA Measurement.................................................................................................................................... 7
3.1 PTSA Measurement............................................................................................................................. 7
3.2 PTSA calibration .................................................................................................................................. 7
4 Fluorescein Measurement ......................................................................................................................... 8
4.1 Fluorescein Measurement .................................................................................................................. 8
4.2 Fluorescein calibration (Firmware version before v1.0r295) ............................................................. 9
4.3 Fluorescein calibration (Firmware version v1.0r295 and after) ....................................................... 10
5 Colorimetric Measurement...................................................................................................................... 11
5.1 Supported Methods.......................................................................................................................... 11
5.2 Select a Method................................................................................................................................ 14
5.3 Single Timing Step Method............................................................................................................... 15
5.4 Single-Vial Procedure........................................................................................................................ 15
5.5 Two-Vial Methods............................................................................................................................. 15
5.6 Multiple Timing Steps Method ......................................................................................................... 16

ii
5.7 Advanced Methods........................................................................................................................... 17
5.7.1 Low range, direct reading chlorine dioxide, 0 to 35.0 ppm....................................................... 17
5.7.2 Turbidimetric Anionic Polymer Method .................................................................................... 17
5.7.3 Direct Reading Bleach Percent Method, 0 to 15%..................................................................... 18
5.8 Method Setup and Calibration.......................................................................................................... 18
5.8.1 Set up the method parameters.................................................................................................. 18
5.8.2 Slope Calibration........................................................................................................................ 19
5.8.3 LowC Calibration ........................................................................................................................ 19
5.8.4 Resume to Default Calibration Parameters ............................................................................... 20
6 Turbidity Measurement........................................................................................................................... 20
6.1 Operation.......................................................................................................................................... 20
6.2 Turbidity Calibration ......................................................................................................................... 20
7 Absorbance Measurement ...................................................................................................................... 21
8 Bluetooth Interface.................................................................................................................................. 22
8.1 Install Software ................................................................................................................................. 23
8.2 Turn on SP-910 Bluetooth................................................................................................................. 23
8.3 Connect uPyxis to SP-910.................................................................................................................. 23
8.3.1 Upgrade Firmware ..................................................................................................................... 23
8.3.2 Setup Product............................................................................................................................. 24
8.3.3 Add User Defined Colorimetric Methods................................................................................... 25
8.3.4. Download Datalog..................................................................................................................... 26
9 Calibrate a ST-500 with SP-910................................................................................................................ 27
10 Maintenance.......................................................................................................................................... 30
11 Troubleshooting..................................................................................................................................... 31
12 Appendix A............................................................................................................................................. 32

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Trademarks and Patents
Hach ® is a registered trademark of the Hach Company, Loveland, CO USA
Confidentiality
The information contained in this manual may be confidential and proprietary and is the property of Pyxis Lab.
Information disclosed herein shall not be used to manufacture, construct, or otherwise reproduce the goods disclosed
herein. The information disclosed herein shall not be disclosed to others or made public in any manner without the
express written consent of Pyxis Lab.
Standard Limited Warranty
Pyxis Lab warrants its products for defects in materials and workmanship. Pyxis Lab will, at its option, repair or
replace instrument components that prove to be defective with new or remanufactured components (i.e., equivalent to
new). The warranty set forth is exclusive and no other warranty, whether written or oral, is expressed or implied.
Warranty Term
The Pyxis warranty term is thirteen (13) months ex-works. In no event shall the standard limited warranty coverage
extend beyond thirteen (13) months from original shipment date.
Warranty Service
Damaged or dysfunctional instruments may be returned to Pyxis for repair or replacement. In some instances,
replacement instruments may be available for short duration loan or lease.
Pyxis warrants that any labor services provided shall conform to the reasonable standards of technical competency
and performance effective at the time of delivery. All service interventions are to be reviewed and authorized as
correct and complete at the completion of the service by a customer representative or designate. Pyxis warrants
these services for 30 days after the authorization and will correct any qualifying deficiency in labor provided that the
labor service deficiency is exactly related to the originating event. No other remedy, other than the provision of labor
services, may be applicable.
Repair components (parts and materials), but not consumables, provided in the course of a repair, or purchased
individually, are warranted for 90 days ex-works for materials and workmanship. In no event will the incorporation of a
warranted repair component into an instrument extend the whole instrument’s warranty beyond its original term.
Shipping
A Repair Authorization Number (RA) must be obtained from the Technical Support ([e-mail]) before
any product can be returned to the factory. Pyxis will pay freight charges to ship replacement or repaired products to
the customer. The customer shall pay freight charges for returning products to Pyxis. Any product returned to the
factory without an RA number will be returned to the customer.

1
1General Description
1.1 Specification
•Colorimeter Wavelength: 365/420/455/525/560/570/630 nm
•Turbidity Excitation Wavelength: White/infrared LED/90-degree scattering
•Fluorescence Excitation Wavelength: 365/460 nm LED
•Fluorescence Emission Wavelength: 410/520 nm
•Wavelength Accuracy: ±1 nm
•Absorbance Reproducibility: 0.005 au (0 - 1.0 au) (3sigma)
•Absorbance Linearity Range: 0 to 1.0 au
•PTSA Reproducibility: 1 ppb PTSA (3 sigma)
•PTSA Detection Limit: 1 ppb
•PTSA Range: 0 - 300 ppb
•Fluorescein Reproducibility: 0.2 ppb or 2% of the value
•Fluorescein Detection Limit: 0.1 ppb
•Fluorescein Range: 600 ppb
•Turbidity Reproducibility: 1 NTU (3 sigma)
•Turbidity Detection Limit: 1 NTU
•Turbidity Range: 0 - 200 NTU
•Battery: 4 AA alkaline
•Typical Battery Life: 3 months
•Display: Graphical LCD 160x240 pixels, visible under direct sunlight
•Instrument Dimension: L 265mm W 88mm H 62mm
•Instrument Weight: 600 g without batteries
•Storage Temperature Range: 0 to 140°F (-18 - 60°C)
•Operation Temperature Range: 40 to 120 °F (4 - 49°C)
•Humidity: 85% at 106 °F (41 °C)
•Environmental: IP67, dustproof and waterproof
Note:
1. Specifications are subject to change without notice with Pyxis’ continuous development.
2. The fluorescein range in earlier versions of the SP-910 may be only up to 20 ppb. To extend the
upper limit to 600 ppb, please contact Pyxis customer support at [e-mail].

2
1.2 Pyxis Major Features
The SP-910 analyzer shown in Figure 1 is a combination of photometer and fluorometer. It provides
colorimetric measurements at 7 LED wavelengths, fluorometric measurement of fluorescent tracer PTSA
and fluorescein, and nephelometric turbidity measurement using white LED and infrared LED as the
excitation sources. The SP-910 is pre-calibrated for colorimetric measurements of analyses common in
industrial water treatment and other water testing in the laboratory or in the field, such as chlorine,
phosphate, iron, and copper. Main features include:
⚫The SP-910 is pre-calibrated for measuring PTSA (pyrenetetrasulfonic acid) in the range of 0 to
300 ppb. The fluorescence PTSA measurement is automatically compensated for sample color
and turbidity interference.
⚫The SP-910 is pre-calibrated for measuring fluorescein in the range of 0 to 600 ppb.
⚫The SP-910 is pre-calibrated for measuring turbidity in the range of 0 to 200 NTU.
⚫Automatically select the primary wavelength according to the method selected and switches to
the secondary wavelength to extend the primary measurement range.
Figure 1. Sample Vial and Major Components
⚫Display a concentration-time profile curve during the last time period in a colorimetric
measurement. The user can terminate the timing process and take a reading if the displayed
concentration reaches a plateau before completing the predefined time period.
⚫The user can update the calibration parameter of any pre-calibrated colorimetric method by
testing a standard solution first and then following a setup procedure to update the calibration
parameters.
⚫Built-in Bluetooth allows easy connection to PC or mobile apps for downloading datalog and
adding new colorimetric methods.
1.3 Unpackaging the Instrument
Remove the instrument and accessories from the shipping container and inspect each item for any
damage that may have occurred during shipping. Verify that all items listed on the packing slip are
included. If any items are missing or damaged, please contact Pyxis Customer Service at service@pyxis-
lab.com.
Navigational Control Pad
LCD
Sample Vial Compartment
Light Shield Cover
Sample Vial

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1.4 Standard Accessories
•Sample Vials - two 10 ml (Part # MA-24), round, 0.78 inch (20 mm) pathlength, glass vials, which can
be used for all measurements including turbidity and fluorescence measurements.
•4 AA alkaline batteries
•Instrument Manual, also available from www.pyxis-lab.com
•25 ml sample via (Part # MA-25)
•16 mm tube adapter (Part # 52214)
•Bluetooth/USB Adapter for Desktop (Part # MA-NEB）
1.5 Optional Accessories
•100 ppb PTSA standard in a 500 ml brown plastic bottle (Part # 21001)
•50,250 and 500 ppb fluorescein standard in a 500 ml brown plastic bottle (Part #s FLUO50, FLUO250,
FLUO500)
1.6 Sample Vial Compartment
The sample vial compartment is shown in Figure 1 along with a 10-ml sample vial. When the sample vial
is inserted into the sample vial compartment, the triangular mark on the sample vial should be aligned
approximately with the 6 o’clock position of the sample vial compartment or any position consistently.
The sample vial compartment can take in a 25 ml sample vial. The light shield cover is not required to be
closed if the 25 ml sample via is used.
The 16 mm tube adapter is needed for colorimetric methods using the 16 mm sample tube. The
instruction
to us the adapter is provided in section 8.
The sample vial compartment should be kept clean. A small amount foreign material could significantly
affect turbidity and fluorescence measurement results. Use a soft cloth or lint free paper tissue to clean
sample vial compartment periodically. Remove debris, scale, and deposit promptly.
1.7 Light Shield Cover
The light shield cover is shown in Figure 2. The light shield cover can be conveniently slid between the
open and closed positions. The light shield cover is held firmly at the rest positions by permanent
magnets.
The light shield cover should be in the closed position during storage, transportation, and measurements,
especially during the turbidity and fluorescence measurements. When turned on, the SP-910 carries out
self-diagnosis including checking the performance of a variety of optical devices. The light shield door
shall be at the closed position to shield interference from ambient light during self-diagnosis.
Care should be taken to avoid water or debris being trapped in the track of the light shield door.
Commented [LR1]: 还有两个 25ml 的瓶子也是标配 MA-25
COD 适配器也是标配
•25 ml sample via (Part # MA-25)
•16 mm tube adapter (Part # 52214)

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Figure 2 Open and Close the Light Shield Cover
Warning
Magnetic sensitive devices, including but not limited to, credit cards, watches, hard disks, should be
keep at a distance of at least 2 inches from the Light Shield Door to avoid possible damage and/or
loss of information recorded.

5
2Start the SP-910
2.1.Battery Installation
The SP-910 is powered by four AA-size alkaline batteries. Do not use rechargeable nickel cadmium
(NiCad) batteries or any AA-size lithium batteries. A set of batteries typically lasts for three months. When
the batteries capacity is low, the SP-910 will prompt a LOW BATTERY warning. Replace all four batteries
to resume operation of the SP-910 after the battery warning.
The SP-910 battery compartment, shown in Figure 3, is on the back side of the instrument. Insert a small
pad underneath the screen area to make the back-surface level when the instrument is turned upside
down. Install batteries as followings:
1. Remove the battery compartment cover by loosening four screws.
2. Insert four batteries into the battery holder as shown in Figure 3. Make sure the positive battery
polarity marker (+) is aligned with the positive marker (+) on the battery holder.
3. Replace the battery compartment cover, making sure that the sealing O-ring is lying flat on the
battery holder and tighten the four screws.
Figure 3 Replace Batteries
2.1 Description of the Navigational Control Pad
The SP-910 navigational control pad consists of five keys as shown in Figure 1. The left, right, up, and
down keys are navigational keys that are used to select an icon, a button, or other items in various pages.
The center key is the OK key. Press the OK key on a selected item to launch the action associated with
the selected item. The OK key is also used to accept the current selection, like the return key in a
computer keyboard.
2.2 Turning on the SP-910
After new batteries installation, the SP-910 will not be automatically turned on. To turn on SP-910, press
the OK key, and release the OK key when the LCD is lit.

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You can navigate the main page menu and launch an operation by pressing on an icon. If battery voltage
is too low for the instrument to work properly, the SP-910 will show a low battery warning message when
it is being turned on If this happens, replace all four batteries.
2.3 Main Page
The SP-910 provides intuitive icon driven user operations. On the main page, eight major feature groups
are illustrated as below:
Figure 4. Main Menu
A brief description of each feature group is given in Table 1. Detailed operation instructions can be found
in the following chapters.
Table 1 Feature Groups on Main Menu
No.
Title
Description
1
M-P
PTSA measurement
2
M-F
Fluorescein measurement
3
COLOR
Colorimetric measurement methods
4
TURB
Turbidity measurement
5
Power
Turn off SP-910
6
ABSB
Absorbance measurements
7
CAL
Calibration routines
8
SYS
System and diagnosis information, Bluetooth enabling
2.4 Turning off the SP-910
Turn the SP-910 off by navigating to Power icon and press the OK key. Alternatively, you can turn off the
SP-910 by pressing OK key for 5 seconds in any menu.
2.5 The SP-910 Auto Power off
The SP-910 automatically turns itself off with no-key activity for a given period, except for during a
measurement. The auto power-off time can be set in SYS->System Set. Pressing OK key will wake up
the instrument, and the SP-910 will return to the original page if it has any measurement data.
2016/07/19 08:00
Power
M-F TURB
ABSB SYS
M-P
CAL
COLOR

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2.6 Auto LCD Power Saving
During a colorimetric method measurement, The SP-910 automatically turns LCD backlight off with no-
key activity and continues the measurement with the LCD backlight off. The auto LCD power-off time can
be set in SYS->System Set. Pressing any key will turn on the LCD backlight. Under normal ambient
lighting condition, icons and other contents shown on the LCD screen are readable without backlight
being on.
3PTSA Measurement
3.1 PTSA Measurement
1. Fill the 10 ml sample vial with the test solution and tightly cap the sample vial.
2. Place the sample vial into the sample vial compartment and slide the light shield cover to the
closed position.
3. Press the M-P on the main page, The SP-910 will start to measure the PTSA concentration
4. The SP-910 will display the PTSA concentration in ppb as PTSA.
3.2 Figure 5. PTSA Measurement
Deionized water (DI) as the blank calibration solution and the 100 ppb PTSA calibration s
During the fluorescence measurement to determine the PTSA concentration, the SP-910 checks the
sample turbidity. If the sample turbidity value detected is greater than 40 NTU, The SP-910 will display a
warning. For best results, the sample should be filtered if turbidity exceeds 40 NTU.
Sample color causes a lower PTSA concentration to be measured. The SP-910 automatically
compensates for sample color. If the sample color is too intense, The SP-910 will display a warning.
For best results, ensure that the sample vial is clean. Wipe off water on the outside wall of the sample vial
using a lint-free tissue paper. Fill the sample vial to the 10 ml mark. If the sample contains air bubbles, tap
the sample vial gently to remove the bubbles before placing the sample vial to sample vial compartment.
3.3 PTSA calibration
tandard solution are needed.
1. Press the CAL on the main page, then choose the M-P and press the OK key to launch the PTSA
calibration page.
2016/07/19 08:00
PTSA
99.8 ppb
Click OK key to measure,
Click other keys to exit!

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2. Follow the message prompts, insert the DI blank into the sample vial compartment and press the
OK key.
3. Follow the message prompts, use the upper and down key to switch between 100 ppb and 200
ppb standard
4. Fill the sample vial with the 100 ppb or 200 ppb standard and place the sample vial into the
sample vial compartment and press the OK key to start calibration
Figure 6. PTSA Calibration
If calibration fails, the followings should be checked:
•The DI blank is being contaminated.
•The 100 ppb PTSA standard solution is decayed or being contaminated.
•The light shield cover is not in the closing position.
•The sample vial compartment is blocked with debris, water, or other materials.
The 100 ppb standard solution shall be stored in a brown or black opaque bottle. Exposing the PTSA
standard to light will cause the standard losing the PTSA concentration. Many substances, such as
quaternary amine cause a negative interference. Many other substances such laundry detergents that
contain optical brightener will cause a significant positive interference.
4Fluorescein Measurement
4.1 Fluorescein Measurement
1. Fill the 10 ml sample vial with the test solution and tightly cap the sample vial.
2. Place the sample vial into the sample vial compartment and slide the light shield cover to the
closed position.
3. Press the M-F on the main page, then press the OK button, The SP-910 will start to measure the
fluorescein concentration in the sample.
4. The SP-910 will display the fluorescein concentration in ppb.

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Figure 7. Fluorescein Measurement
For best results, ensure that the sample vial is clean. Wipe off water on the outside wall of the sample vial
using a lint-free tissue paper. Fill the sample vial to the 10 ml mark. If the sample contains air bubbles, tap
the sample vial gently to remove the bubbles before placing the sample vial to sample vial compartment.
4.2 Fluorescein calibration (Firmware version before v1.0r295)
Deionized water (DI) as the blank calibration solution and the 20 ppb fluorescein calibration standard
solution are needed.
1. Press the CAL on the main page, then choose the Fluorescein and press the OK key to launch
the fluorescein calibration page.
2. Follow the message prompts, insert the DI blank into the sample vial compartment and press the
OK key.
3. Follow the message prompts and insert the 20 ppb standard into the sample vial compartment
and press the OK key.
4. Press the OK key to return to the main page.
Figure 8. Fluorescein Calibration
If calibration fails, the followings should be checked:
•The DI blank is being contaminated.
•The 20 ppb fluorescein standard solution is decayed or being contaminated.
•The light shield cover is not in the closing position.
•The sample vial compartment is blocked with debris, water, or other materials.
2016/07/19 08:00
M-F Calibration
Insert 0.0 ppb standard!
7730
Insert 20.0 ppb standard!
Click OK key to continue!
Click up and down key to change!

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4.3 Fluorescein calibration (Firmware version v1.0r295 and after)
Deionized water (DI) as the blank calibration solution, the 50 ppb fluorescein, the 250 ppb fluorescein and
the 500 fluorescein calibration standard solutions are needed.
1. Press the CAL on the main page, then choose Fluorescein and press the OK key to launch the
fluorescein calibration page.
2. Follow the message prompts, insert the DI blank into the sample vial compartment and press the
OK key.
3. Insert the 50 ppb standard into the sample vial compartment and press the OK key to complete
the low range calibration.
4. Press the OK key to proceed with middle range calibration or press any other keys to return to
main page.
5. Insert the 250 ppb standard into the sample vial compartment and press the OK key to complete
the middle range calibration.
6. Press the OK key to start proceed with high range calibration or press any other keys to return to
main page.
7. Insert the 500 ppb standard into the sample vial compartment and press the OK key to complete
the high range calibration.
The middle range and high range calibrations from steps 4 to 8 are optional if only low range
fluorescein measurement is intended.
Figure 9. Low Range Fluorescein Calibration
Figure 10. Middle Range Fluorescein Calibration

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Figure 11. High Range Fluorescein Calibration
The standard solutions shall be stored in a brown or black opaque bottle. Exposing the fluorescein
standard to light will cause the standard losing the fluorescein concentration. Many substances, such as
quaternary amine cause a negative interference. Many other substances such laundry detergents that
contain optical brightener will cause a significant positive interference.
5Colorimetric Measurement
5.1 Supported Methods
A wide range of colorimetric methods is supported by the SP-910 analyzer and the number of them keeps
increasing with continuous development of Pyxis. See corresponding Hach® methods in Appendix A.
Table 2 List of Supported Colorimetric Methods
Abbreviated
Method Name
Method Name
Description
Range
AL
Alumi
Aluminum,Aluminon method
0.8 ppm
ALKLR
Alkalinity
Alkalinity, Total, Low Range
100 ppm as
CaCO3
ALKHR
Alkalinity
Alkalinity, Total, High Range
500 ppm as
CaCO3
AZOL
Azole
UV digestion for tolyltriazole and benzotriazole
16 ppm
BLCH
Bleach
Direct method measuring sodium hypochlorite
concentration
16%
BLCHL
Bleach
Direct method measuring sodium hypochlorite
concentration, Low Range
1.50%
Br-T
Bromine
Bromine,DPD method for
4.5 ppm
Ca
Ca
Calcium ,Calmagite method
4 ppm as
CaCO3
CaHR
Ca
Calcium hardness ,Murexide method
500 ppm as
CaCO3
CaMgL
CaMg
Total hardness,Chlorophosphonazo
colorimetric method , Ultra-Low Range
1 ppm as
CaCO3
CODLR
COD
Oxygen Demand, Chemical (Reactor
Digestion 20 Minutes Method) ,Low range
150 ppm

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Abbreviated
Method Name
Method Name
Description
Range
CODHR
COD
Oxygen Demand, Chemical (Reactor
Digestion 20 Minutes Method) , High range
1500 ppm
CODUH
COD
Oxygen Demand, Chemical (Reactor
Digestion 20 Minutes Method) ,Ultra-High
Range
15000 ppm
CODUL
COD
Oxygen Demand, Chemical (Reactor
Digestion 20 Minutes Method) ,Ultra-Low
Range
40 ppm
CLLR
CLLR
Turbidimetric method,Low Range
40 ppm
CLMR
CLMR
Turbidimetric method ,Medium Range
400 ppm
CL2HR
CL2High
Total Chlorine, DPD method , High Range
10 ppm
CL2HR
CL2High
Free Chlorine, DPD method , High Range
10 ppm
CL2UH
CL2UH
Free Chlorine, Iodimetr method ,Ultra-High
Range
400 ppm
CL-F
F-Chlorine
Free chlorine, DPD method
2.2 ppm
CLTMB
Chlorine, Free
Free chlorine ,TMB method
1.2 ppm
ClO2
ClO2-DPD
DPD method, USEPA accepted for reporting
drinking water analysis
5 ppm
ClO2D
ClO2Direct
Direct method for chlorine dioxide,Medium
Range
45 ppm
CLO2H
ClO2Direct
Direct method for chlorine dioxid, High Range
1500 ppm
CL-T
T-Chlorine
Total chlorine, DPD method
2.2 ppm
CN
Cyanide
Cyanide,Pyridine-Pyrazalone method
0.24 ppm
COLOR
Color
Color, APHA Platinum-Cobalt Standard
Method
500 units
Cr6
Cr6
chromium hexavalent,1,5-
Diphenylcarbohydrazide method , USEPA
accepted for wastewater analyses
0.6 ppm
CrT
CrTot
Total Chromium ,Alkaline hypobromite
Oxidation method
0.6 ppm
CuBi
Cu_Bicinch
Bicinchoninate method, EPA approved for
reporting wastewater analysis
5 ppm
CuLR
CuPorp
Copper,Porphyrin method
0.21 ppm
CYAN
CYAN
Cyanuric acid,Turbidimetric method
55 ppm
CYN-F
Cyclohexylamine
Cyclohexylamine,Fluorescent method
1.2 ppm
DEHA
DEHA
Iron Reduction method for N,N-
diethylhydroxylamine and other oxygen
scavengers
0.5 ppm
DO
DO
Dissolved Oxygen,Iodimetry method
10 ppm
F
Floride
Fluoride, SPADNS method
2 ppm
FeMo
FeMo
Total iron method for water containing
molybdate
1.8 ppm
FePh
Fe_phenanth
Total iron using 1,10-phenanthroline, USEPA
approved for reporting wasterwater analysis
3 ppm
FeSal
Fe-Sal
Total Iron using 5-Sulfosalicylic Acid
Dihydrate
5 ppm
FeTp
FeTptz
Total iron using TPTZ
1.8 ppm
FeZi
FeZine
Total iron , FerroZine method
1.3 ppm

13
Abbreviated
Method Name
Method Name
Description
Range
H2O2
H2O2
Hydrogen peroxide,Iodimetry method
500 ppm
H2O2L
H2O2L
Hydrogen peroxide, DPD method,Low Range
1.5 ppm
Mg
Mg
Magnesium,Calmagite method
4 ppm as
CaCO3
MnHR
MnHigh
Manganese,Periodate Oxidation method,High
range
20 ppm
MnLR
MnLow
Manganese,Periodate Oxidation method,Low
range
0.7 ppm
MoHR
Mo_HighRange
Molybdate, Mercaptoacetic Acid method,High
range
40 ppm
MoLR
Mo_LowRange
Molybdate, ternary complex method,Low
range
3 ppm
N2H4
N2H4
Hydrazine,p-Dimethylaminobenzaldehyde
method
0.5 ppm
NH2C
NH2CL
Chloramine mono ,Indophenol method
3 ppm
N-TLR
N-TLR
Nitrogen, Total (Test ‘N Tube Method) ,Low
range
25 ppm as N
N-THR
N-TLR
Nitrogen, Total (Test ‘N Tube Method) ,High
range
150 ppm as N
NH3S
NH3Sal
nitrogen, ammonia ,Salicylate method
0.5 ppm as N
NH3-F
NH3-F
Ammonia Nitrogen ,Fluorescent Method
0.07 ppm as N
NH3LR
NH3LR
Nitrogen, Ammonia (Test ‘N Tube) - Low
range
2.5 ppm as N
NH3HR
NH3HR
Nitrogen, Ammonia (Test ‘N Tube) -High range
50 ppm as N
Ni
Ni
Nickel,PAN method
1.2 ppm
NO2D
NO2D
Direct method for nitrite
1000 as NO2
NO2H
NO2H
High range nitrite, ferrous sulfate method
150 as NO2
NO2L
NO2L
Low range nitrite, diazotization method,
USEPA approved for reporting wastewater
and drinking water analysis
0.35ppm as
NO2
NO3HR
NO3H
Nitrate ,Cadmium Reduction method,High
range
30 ppm as N
NO3MR
NO3M
Nitrate ,Cadmium Reduction method,Middle
range
5 ppm as N
NO3CA
NO3CA
Nitrate, High Range (Test ‘N Tube Method)
30 ppm as N
O3
O3
Ozone ,DPD method
2 ppm
PAA
PAA
Peroxyacetic , Iodimetry method
500 ppm
OPO4
OPO4
Reactive phosphate using ascorbic acid
molybdenum blue method, USEPA accepted
for wastewater analysis
2.5 as PO4
OrgP
Phosphonate
UV digestion and ascorbic acid reduction
molybdenum blue method
2.5 as PO4
PAmi
OPO4-Amino
Reactive phosphate, amino acid reduction
method
30 as PO4
P-TLR
P-TLR
Phosphorus, Total (Test ‘N Tube Method) -
Low range
3.5 as PO4
P-THR
P-THR
Phosphorus, Total (Test ‘N Tube Method) -
High range
100 as PO4

14
Abbreviated
Method Name
Method Name
Description
Range
pH
pH
Phenol red method for pH
8.5
PMoV
OPO4-MoV
Reactive phosphate , Molybdovanadate
method
45 as PO4
POLY
Polymer
Anionic polymeric dispersant,Turbidimetric
method
14 ppm
Sb3+
Sb3+
Antimony Trivalent ,PADAP Method
0.11 ppm
Sb-T
Sb-T
Antimony, Total ,PADAP Method
0.11 ppm
S2-
Sulfide
Methylene blue method for sulfide, USEPA
accepted for reporting wastewater analysis
0.7 ppm
SiHR
SiHR
Silica, Silicomolybdate method,High Range
75 as SiO2
SiLR
SiLR
Silica,Heteropoly Blue method,Low Range
5 as SiO2
SO3LR
SO3LR
Sulfite,OPA method ,Low Range
5 ppm
SO3HR
SO3HR
Sulfite,OPA method , High Range
50 ppm
SO4
SO4
Barium sulfate, Turbidimetric method
70 ppm
TOC
TOC
Total Organic Carbon
20 ppm
Urea
Urea
Urea (Reactor Digestion Method)
10 ppm
ZnXO
ZnXO
Zinc ,Xylenol orange method
3 ppm
Zn
Zinc
Zincon method for zinc, USEPA approved for
wastewater analysis
3 ppm
5.2 Select a Method
Move the icon focus to the method icon COLOR using the navigational (left, right, up, or down) keys.
Press OK on the icon to launch the first method selection page. The methods shown on the top row of the
page are the most frequently selected methods.
Figure 12. Method Selection
The followings are the operations associated with this page:
1. Use the navigational keys and the OK key to select and launch a method.
2. Long press the OK key to return to the main page. Press the arrow icon at the lower right corner
of the page to display the second method selection page if the device is loaded with more than 23
methods.
Note: Methods shown in the method selection pages include Hach© equivalent methods and Pyxis
specific advanced methods. The table in Appendix A provides a brief description of Pyxis method names

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and their corresponding Hach® program number. Hach® reagents for 10 ml sample can be used for the
test.
5.3 Single Timing Step Method
Most of colorimetric methods have only one timing step. As an example, in the DPD free chlorine method,
it takes one minute for the DPD powder reagent to completely react with chlorine in the water sample.
The DPD free chlorine method has a single one-minute timing step. Figure 13 shows the main page of a
method with a single timing step.
Figure 13. Single Timer Method
5.4 Single-Vial Procedure
1. Place the sample vial filled with the water sample in the SP-910 sample vial compartment and
press the ZERO button. The SP-910will display the page shown in Figure 13.
2. Take the sample vial out and add the reagent to the sample vial.
3. Place sample vial back into the sample vial compartment and press the timer button TMR1. The
SP-910 will start to monitor the reaction between the reagent and the species you want to
measure in the water sample. The concentration is shown in the chart as a function of time
(Figure 14).
4. When the timer reaches the preset time and the reaction is complete, the value of concentration
will be shown on the top right corner of the page.
5. The rate of the reaction is often faster than the standard pre-set time, which will become apparent
from the concentration-time plot. You can press the STOP button to stop the timer and terminate
the timing step. The last read concentration value will be displayed on the top right corner of the
page after you terminate the timing step.
Figure 14. Concentration as a Function of Time
5.5 Two-Vial Methods

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Some colorimetric methods require using two vials. The water sample is added to two identical vials. One
vial is being used to zero the colorimeter, referred as to the prepared blank. A reagent is added to the
other vial, referred as to the prepared sample. The absorbance value is determined from the prepared
sample.
If the method requires two or more reagents, the prepared blank could be the resulting solution after one
or more reagents have been added to the sample.
The following procedure is typical for two-vial methods:
1. Place the prepared blank into the SP-910 sample vial compartment and press the ZERO button
to zero the instrument.
2. Place the prepared sample into the SP-910 sample vial compartment and press the TMR1 button
to start the method timer.
3. When the timing step is completed, the measured concentration will be displayed on the top of
the page. The timing step could be terminated earlier by pressing STOP button.
4. Optionally, the SP-910 can be re-zeroed using the prepared blank after the timing step is
completed or terminated. The blank reading will be subtracted from the measured concentration
value, and the displayed concentration value on the top-right corner will be updated. This step is
optional. It is only necessary if the prepared blank changes its color during the timing period.
5. Optionally, the prepared sample vial can be put back and read again by pressing the READ
button if the blank is re-zeroed after the timing step is completed or terminated. A new
concentration value based on the last absorbance value measured will be calculated and
displayed.
5.6 Multiple Timing Steps Method
Some colorimetric methods have two or three timing steps. The SP-910 shows a count-down timer for the
timing steps before the last timing step (Figure 15). During these timing steps, one or more reagents are
added to the sample, or operations such as swirling the vial to mix the reagent and the sample are being
performed. These methods usually use one vial for the prepared blank and the other for the prepared
sample.
Figure 15. Multiple Timer Method
In order to show the concentration-time curve as shown in Figure 14 during the last timing step, The SP-
910 must be zeroed using the prepared blank before the last timing step. Thus, the last timer button will
not be selectable until the SP-910 has been zeroed using the prepared blank. Multi-timing step Hach®
methods require zeroing the colorimeter using the prepared blank after the last timing step is completed.
The SP-910 can optionally be re-zeroed using the prepared blank after the last timing step. The blank