Fisher Scientific accuSpin 1 User manual

accuSpin™ 1 / 1R
OPERATING
INSTRUCTIONS

How to use this manual
Use this manual to get acquainted with your centri-
fuge and its accessories.
The manual helps you to avoid inappropriate han-
dling. Make sure to keep it always close to the centri-
fuge.
A manual that is not kept handy cannot provide
protection against improper handling and thus
against damage to persons and objects.
The manual comprises chapters on
• Safety regulations
• Instrument description
• Rotor program and accessories
• Transportation and hook-up
• Use of the centrifuge
• Maintenance and care
• Troubleshooting
• Technical data
• Index
Please fold out
Overleaf you will find a graphic
representation of the control panel
with a survey of the most important
functions

speed/RCF
Quick run
run time
open lid
stop
start
(only for instruments with refrigeration unit)
temperature
Bucket selection
rpm/RCF
switch key
display
"set" keys
"set" keys
Before switching on the centrifuge
please read this manual
acceleration / deceleration
profiles

Control panel of the accuSpin™ 1 / 1R
Display panels
Program selection
acceleration / deceleration profiles:
1 = slow acceleration – brake off
2 = fast acceleration – brake on
(see appendix page 69)
Speed / RCF
run: current value of speed or RCF after activation of
switch key
lid open: "OPEN"
(before start)
lid closed: "0"
error code: will flash in display
Run time
time selection: - remaining run time to “0”
continuous - run time passed (hLd)
operation (hLd) (in hours, minutes)
"Quick Run": - run time passed (as long as button is held;
in minutes and seconds)
Temperature*
run: current sample temperature in °C
(in temperature equilibrium)
* only for instruments with refrigeration unit
Keys
start : normal start of the centrifuge
stop : manual stop of a run
open lid: open lid (possible only with the instrument
switched on)
Quick run: short-term operation of the centrifuge as long as
key remains pressed
rpm/RCF
switch: switching between rpm and RCF display
rotor / bucket set.: Setting of the rotor / bucket number
"set" keys: stepwise increase/decrease of setpoint values
Short pressing of any of the "set" keys: switch from current to preset
value, signaled by flashing display.
Error codes (troubleshooting see chapter "Troubleshooting"):
E-03: speed measuring is disturbed during run
E-07: Overtemperature alarm
E-17: lid locking mechanism is clamping
E-21: insufficient acceleration power
(e.g. in case of tremendous imbalance)
Lid : lid switch protection circuit has released
bAL : unbalance

Contents
1
Contents
For your safety............................................ 3
Proper use................................................................ 3
Improper use ............................................................ 3
Centrifuging hazardous materials ............................ 3
Handling the centrifuge ............................................ 4
Conformity to current standards............................... 5
Safety instructions in this manual ............................ 5
The accuSpin™ 1 / 1R ................................ 7
Description ............................................................... 7
Safety systems......................................................... 8
Parts supplied .......................................................... 8
Function and features .............................................. 9
Before use................................................. 11
Centrifuge transport and installation ...................... 11
Proper location ....................................................... 11
Main connection ..................................................... 12
Rotors and accessories ........................... 13
Rotors for the accuSpin™ 1................................... 14
Rotors for the accuSpin™ 1R................................. 16
Handling the rotor .................................... 21
Swinging Bucket Rotor 7500 3450......................... 21
Sealed Rotor 7500 3454 ........................................ 22
Check list for operation with a Sealed Rotor ...... 25
Aerosol-tight operation ........................................... 27
Checking of aerosol-tight bio-containment ......... 28
Operation .................................................. 29
Switching on the centrifuge .................................... 29
Actuating the lid...................................................... 29
Opening the lid ................................................... 29
Closing the lid ..................................................... 29
Installing the rotor................................................... 30
Loading the rotor .................................................... 32
Maximum loading ............................................... 32
Filling the centrifuge tubes ................................. 33
Maximum permissible load difference ................ 33
Inserting the centrifuge tubes ............................. 34
Loading instructions for Sealed Rotor.................... 36
Entering parameters............................................... 39
Rotor / Bucket selection ......................................... 39
Deceleration curves ............................................... 39
Brake cut-out speed selection................................ 39
Selecting speed...................................................... 40
Switching from speed to RCF display .................... 41
Entering the RCF value.......................................... 41
More about the RCF value ................................. 41
Selecting run time .................................................. 42
Run time selection .............................................. 42
Continuous operation ......................................... 42

Contents
2
Selecting the temperature ...................................... 43
Starting the centrifuge ............................................ 43
Imbalance display................................................... 43
Changing the settings during the run ..................... 44
Stopping the centrifuge .......................................... 44
Stopping with preset run time............................. 44
Stopping with continuous operation ................... 44
”Quick Run” ............................................................ 44
Removing the rotor................................................. 45
Audible alarm ......................................................... 45
Turning the centrifuge off ....................................... 45
Maintenance and care .............................. 47
Maintenance to be performed by the customer ..... 47
Cleaning.............................................................. 47
Disinfection ......................................................... 48
Decontamination................................................. 50
Autoclaving ......................................................... 50
Sealed Rotor 7500 3454 ........................................ 51
Cleaning.............................................................. 51
Decontamination................................................. 52
Troubleshooting ....................................... 55
Emergency lid release............................................ 55
Error troubleshooting.............................................. 57
Contacting Fisher ScientificService..................... 63
Technical Data .......................................... 65
Electrical connections / fuses ................................. 67
Appendix ................................................... 69
Acceleration / deceleration profiles ........................ 69
Speed/RCF diagrams............................................. 77
Index .......................................................... 81

For your safety
3
For your safety
Fisher Scientificcentrifuges are manufactured accord-
ing to current technical standards and regulations.
Nonetheless, centrifuges may pose danger to individu-
als and surrounding if
• they are not used as designed
• they are operated by untrained personnel
• their design is improperly changed
• the safety instructions are not followed
Therefore, personnel involved with operation and
maintenance of the centrifuge must read and follow
the safety instructions.
In addition, the pertinent regulations for prevention of
accidents must be strictly followed
This manual is an integral part of the centri-
fuge assembly and must be kept close at
hand at all times.
When damages at the power cord or at
casing were noticed the centrifuge has
to be set out of operation!
Proper use
The centrifuge is designed to separate liquid-
suspended materials having different densities and
particle size, respectively (maximum sample density is
1.2 g/cm³ {ml} at maximum speed).
Improper use
During a run, a safety zone of 30 cm (11.8 inches)
around the centrifuge must be maintained where nei-
ther persons nor hazardous materials may be present.
The centrifuge may cause harm to user or other per-
sons or may damage goods if safety measures are not
followed:
Centrifuging hazardous materials
• The centrifuge is neither made inert, nor is it explo-
sion-proof. Therefore never use the centrifuge in an
explosion-prone environment.
• Do not centrifuge explosive or flammable sub-
stances. The same holds for substances prone to
react violently with each other.

For your safety
4
• Do not centrifuge toxic or radioactive substances or
pathogenic microorganisms without suitable safety
systems.
If microbiological samples of risk group II (according
to "Laboratory Bio-safety Manual" of WHO) are be-
ing centrifuged, aerosol-tight bio-seals have to be
used.
For materials with a higher risk group, more than
one precaution is required.
• Should toxins or pathogenic substances enter the
centrifuge or its parts, you must perform appropriate
procedures for disinfection (see "Maintenance and
care – Disinfection").
• Strongly corrosive substances that may cause
damage to materials and reduce the mechanical
strength of the rotor, may be centrifuged only inside
protective tubes.
Handling the centrifuge
• Use only original accessories for the centrifuge. The
only exception are common glass or plastic centri-
fuge tubes, if they are approved for the rotor speed
and RCF values.
• Never use the centrifuge unless the rotor is properly
installed.
• You may use the centrifuge only with a properly
loaded rotor. You must not overload the rotor.
• Strictly follow the rules and regulations for cleaning
and disinfection
• If the rotor or the rotor lid shows signs of corrosion
or wear, you must stop using it.
• Never open the lid manually if the rotor still turns.
• You may use the emergency lid release only in
case of emergency, e.g. during an interruption of
power supply
(see chapter "Troubleshooting").
• Never use the centrifuge with the lid open.
• Never use the centrifuge if the front panel has been
partially or totally removed.
• Changes in mechanical or electrical components of
the centrifuge may be carried out only by individuals
authorized by Fisher Scientific.

For your safety
5
Conformity to current standards
Fisher Scientificcentrifuges are manufactured and
tested according to the following standards and regula-
tions:
- for all voltages
• IEC 61010
- for 120 V only
•
- for 230 V only
•
Details of the test standards take you please from the
technical data.
Safety instructions in this manual
This symbol denotes potential hazards to
persons.
This symbol denotes potential damage to
the centrifuge or parts in its immediate
surroundings.
General hints are marked with this
symbol.

For your safety
6
Notes

Description of the accuSpin™
7
The accuSpin™ 1 / 1R
The figure below shows a accuSpin™ 1R with the lid
open and a swinging bucket rotor installed.
Description
The accuSpin™ 1R is a general-purpose tabletop cen-
trifuge for biotechnological and pharmaceutic research
that moves high capacity centrifugation onto the fast
track. It spins more tubes, at higher RCFs, more rapidly
than competitive instruments, and can process nomi-
nally one liters of sample in a single run. There are
various rotors with high RCF values that can accom-
modate a wide range of accessories for all common
tube types, microtiter plates and deep-wells.
The user-friendly "Easycontrol" control panel permits
easy selection of speed, RCF value, run time and run
profile (acceleration and deceleration), as well as tem-
perature of the accuSpin™ 1R. You can switch from
speed to RCF display and vice versa, with a touch of a
button and even during a run.

Description of the accuSpin ™
8
Safety systems
The accuSpin™ is equipped with a number of safety
systems:
• Housing and lid is constructed of 8 mm steel.
• Lid with window
• Lid lock with safety check
You can open the centrifuge lid only when the
power is turned on and the rotor has come to a
stop. You can start the centrifuge only if the lid is
properly locked.
Do not tamper with the safety
system!
Parts supplied
Accessories supplied with the centrifuge are:
− power cord
− collet chuck (Cat.-No. 70003100)
− a special wrench for securing the rotor
− corrosion protective oil
The printed documents consist of the delivery notes
and this Manual.

Description of the accuSpin™
9
Function and features
Basic unit/ function Description / feature
Cabinet / frame galvanized steel
Chamber stainless steel
Drive Brushless induction drive
Key pad and display key pad and display elements covered by an easy-care continues surface
Control Microprocessor driven by "Easycontrol II"
Main memory Recalls last run parameters
Program memory data are stored until new values are entered.
Advanced features RCF-programming, quick run, temperature control during standby *
Acceleration / deceleration profiles 2 acceleration / deceleration profiles
( * only with refrigeration unit )

Description of the accuSpin ™
10
The Easycontrol user interface
Function Feature
Acceleration / deceleration profile 1 = slow acceleration – brake off
2 = fast acceleration – brake on
Setting speed by rpm adjustable from 300 rpm to 15 000 rpm (depending on the rotor),
- in 10 rpm increments
RCF selection upon activation of RCF switch , the RCF value can then be entered
Time selection adjustable in minutes from 1 min to 9 h 59 min, and with an increment
of 1 hour from 10h up to 99 h – ”hLd“-mode: continuous operation
Run time display in "quick run“ mode between 1 s and 60 s in seconds´ steps, above in minutes´ steps
Setting temperature
(only with refrigeration unit)
adjustable from 0°C to +40°C, in one degree increments
Lid opening automatic unlocking via ”Open lid“ key ( )
(unlocking in case of power failure: see chapter ”Troubleshooting“)
Start start key ( )
Stop stop key (
)
"Quick Run“ mode pressing the "Quick Run“ key ( ) activates maximum acceleration up to the
maximum permissible speed of rotor; upon key release centrifuge stops with
maximum deceleration power.
Diagnostic messages general instrument malfunction
(error messages with ERROR codes, see “Troubleshooting“)

Before use
11
Before use
Centrifuge transport and installation
After opening the box remove the protective materials.
When transporting the centrifuge,
consider its weight (see „Technical
Data“); always grab it on both sides
taking care that enough helpers are
around.
Do not lift on the front panel!
The centrifuge can be damaged by
jolting during the transport!
Transport the centrifuge only in the
upright position using proper con-
tainment and secure it properly. Han-
dle the centrifuge carefully.
Proper location
The centrifuge may only be used indoors. Its location
must meet the following criteria:
• A safety zone of at least 30 cm (12 inches) around
the centrifuge must be maintained. Hazardous ma-
terials must not be stored beside unit while
centrifugation.
• The laboratory bench or centrifuge trolley must be
stable and resonance-free. A good support is pro-
vided by a laboratory bench or a centrifuge cart with
lockable casters.
• To ensure sufficient air circulation, a minimum dis-
tance from the wall of 10 cm (4 inches) at the back
and of 15 cm (6 inches) on each side must be kept.

Before use
12
• The centrifuge must be protected from heat and
direct sunlight.
• The location must be well ventilated at all times.
UV rays reduce the durability of plas-
tics.
Protect the centrifuge, rotors and
plastic accessories from direct
sunlight.
Main connection
Connect the centrifuge only to an grounded main
power supply. Make sure that the power cord is com-
patible with the safety regulations valid, and that your
main voltage and frequency correspond to the specifi-
cations on the instrument label.
Make sure that the centrifuge is switched off before
connecting electrical wire to main power supply.
specifications
Main connection
mains switch

Rotors and accessories
13
Rotors and accessories
A rotor is not included as part of a accuSpin™ 1 or 1R
centrifuge.
In addition, there are adapters and reduction sleeves
for a variety of commercially available tubes and bot-
tles.
Please consult our sales documentation for a complete
collection of accessories including technical data and
order numbers.

Rotors and accessories
14
Rotors for the accuSpin™ 1
Table 1:
Rotor designation Swinging Bucket Rotor 7500 3450
With bucket
Order no.
Round bucket
400 ml
7500 3451
Rectangular bucket
150 ml
7500 3452
Carrier for
microplates
7500 3453
Maximum permissible load [ g ] 4 x 600 4 x 510 4 x 450
Maximum speed nmax [ rpm ] 4000 4000 4000
Maximum RCF value at nmax 3345 3220 2773
Radius (max.) [ cm ] 19.0 18.1 15.5
Acceleration / deceleration time [ s ] 60 / 55 60 / 55 60 / 55
Aerosol-tight yes* (with cap 75007584) yes no
Autoclavable no no no
* Checked by CAMR, Porton-Down, UK

Rotors and accessories
15
Table 1:
Rotor designation
Order no.
Sealed Rotor
48 x 15 ml
7500 3454
Maximum permissible load [ g ] 4 x 510
Maximum speed nmax [ rpm ] 6000
Maximum RCF value at nmax 6238
Radius (max.) [ cm ] 15.5
Tube angle [ ° ]
Acceleration / deceleration time [ s ] 95 / 95
Aerosol-tight yes
Autoclavable 121°

Rotors and accessories
16
Rotors for the accuSpin™ 1R
Table 2:
Rotor designation Swinging Bucket Rotor 7500 3450
With bucket
Order no.
Round buckets
400 ml
7500 3451
Rectangular buckets
150 ml
7500 3452
Carrier for
microplates
7500 3453
Maximum permissible load [ g ] 4 x 600 4 x 510 4 x 450
Maximum speed nmax [ rpm ] 4000 4000 4000
Maximum RCF value at nmax 3345 3220 2773
Radius (max.) [ cm ] 19.0 18.1 15.5
Acceleration / deceleration time [ s ] 60 / 55 60 / 55 60 / 55
Min temperature at nmax
relative to room temperature 23°C
< 4°C < 4°C < 4°C
Speed at 4°C [ rpm ] 4000 4000 4000
Aerosol-tight yes* (with cap 75007584) yes no
Autoclavable no no no
* Checked by CAMR, Porton-Down, UK