R-Biopharm Rida Cycler User manual

R-Biopharm AG
RIDA®CYCLER
User Manual
BILD

RIDA®CYCLER Version 1.0 | User manual
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RIDA®CYCLER Version 1.0 | User manual
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RIDA®CYCLER Version 1.0 | User manual
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All rights reserved.
This User manual may be used only for its intended purpose. It may not be reproduced in
whole or
in part or be translated into another language without our express, prior written consent.
Subject to technical changes.
Technical changes, deviations in illustrations and errors reserved.
©2018 R-Biopharm AG, Darmstadt
User manual
RIDA®CYCLER
ZRCYCLER
Version 1.0 (2017-12-14)
© Copyright 2018 by R-Biopharm AG
Distributed by
R-Biopharm AG
An der neuen Bergstraße 17
64297 Darmstadt, Germany
Phone: [phone] - 8102-0
Fax: [phone] - 8102-40
E-mail: [email protected]
www.r-biopharm.com

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User manual

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Inhaltsverzeichnis
General information ............................................................................................................ 9
Important information ......................................................................................................... 9
Explanation of symbols used............................................................................................. 9
Intended use.......................................................................................................................13
1 Unpacking and installation.............................................................................................14
1.1 Hardware installation.....................................................................................................................14
1.2 Software installation ......................................................................................................................14
1.3 Updating software..........................................................................................................................16
1.4 Upgrading firmware........................................................................................................................16
2 RIDA®CYCLER: Overview ...............................................................................................17
3 Consumables and Accessories......................................................................................18
4 LED Indicator Colours.....................................................................................................18
5 Getting started.................................................................................................................19
5.1 Loading tubes.................................................................................................................................19
5.2 Removing tubes..............................................................................................................................22
6 Software overview...........................................................................................................22
6.1 Tool bar ...........................................................................................................................................23
6.1.1 Help icon ......................................................................................................................................23
6.1.2 Open run files ..............................................................................................................................24
6.1.3 Instrument icon............................................................................................................................24
6.1.4 Instrument Communication Icon ...............................................................................................26
6.2 File tab.............................................................................................................................................27
6.3 Navigator bar ..................................................................................................................................27
6.4 File active windows........................................................................................................................28
6.4.1 CSV Export or Copy to Clip Board ............................................................................................30
6.4.2 Export Raw Data..........................................................................................................................30
6.4.3 Export Chart as Bitmap ..............................................................................................................30
6.4.4 Result Table Organization..........................................................................................................31
6.4.5 Sizing Bars...................................................................................................................................32
7 Samples Selector.............................................................................................................33
7.1 Samples Selector Grouping ..........................................................................................................35
8 Creating a New Run.........................................................................................................36
8.1 Adding assays ................................................................................................................................36
8.2 Assay profile compatibility............................................................................................................38
8.3 Updating Changes to an Assay ....................................................................................................39
Contents

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9 Starting the Run...............................................................................................................40
9.1 Samples Editor ...............................................................................................................................42
9.1.1 Well layout....................................................................................................................................42
9.1.2 Filling cells...................................................................................................................................42
9.1.3 Colors ...........................................................................................................................................43
9.1.4 Name.............................................................................................................................................43
9.1.5 Type ..............................................................................................................................................44
9.1.6 Sample Concentrations ..............................................................................................................46
9.1.7 Multiplex Standards ....................................................................................................................48
9.1.8 Linking an assay to a sample.....................................................................................................48
9.1.9 Optional columns........................................................................................................................49
9.1.10 Import samples..........................................................................................................................49
9.1.11 Sample editor warnings............................................................................................................50
9.1.12 Lock down samples editor .......................................................................................................50
9.1.13 Information.................................................................................................................................50
10 Creating templates ........................................................................................................52
10.1 Create a template..........................................................................................................................52
10.2 Opening a template......................................................................................................................52
11 During a run...................................................................................................................52
11.1 Modifying a profile during a run (optional)................................................................................53
11.2 Data................................................................................................................................................53
11.3 Messages ......................................................................................................................................54
12 Analysis..........................................................................................................................54
12.1 Cycling analysis ...........................................................................................................................55
12.1.1 Graph types................................................................................................................................55
12.1.2 Cycling analysis parameters....................................................................................................57
12.2 Cycling analysis results table.....................................................................................................59
12.2.2 Floating windows ......................................................................................................................60
12.3 Standard curve using absolute quantification analysis...........................................................61
12.3.1 Efficiency calculation................................................................................................................62
12.3.2 Standard curve results table....................................................................................................63
12.3.3 Cycling analysis for standard curves .....................................................................................64
12.4 Absolute quantification ...............................................................................................................65
12.4.1Sample results table ..................................................................................................................65
13 Reports...........................................................................................................................66
13.1 Report configuration....................................................................................................................66
13.2 Report preview..............................................................................................................................67
13.2.1 Run properties...........................................................................................................................67
13.2.2 Samples......................................................................................................................................68
13.2.3 Analysis......................................................................................................................................68
13.3 Report options..............................................................................................................................69

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14 Excel workbook.............................................................................................................70
Notes and Annex................................................................................................................72
Technical data, ambient conditions ...................................................................................................72
Biological Safety information..............................................................................................................73
Decontamination of Instrument ..........................................................................................................73
EC Declaration of conformity/CE Mark ..............................................................................................73
Type Plate Symbols..............................................................................................................................74
Disposal Information............................................................................................................................74
Annex 1: Temperature verification system (TVS) .............................................................................75
Annex 2: Error messages and warnings............................................................................................76
Annex 3: Toshiba Bluetooth®work around .......................................................................................77
Annex 4: CFR 21, Part 11.....................................................................................................................78
Annex 5: Dye colour chart...................................................................................................................81
Acknowledgement of Registered Trademarks..................................................................................83
References ............................................................................................................................................84
Abbreviations........................................................................................................................................85
Glossary ................................................................................................................................................86

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Please carefully compare the contents of this delivery with the enclosed delivery note, the
package leaflet or the invoice. We recommend that you keep a copy of this document together
with the instructions, so that you can quickly access information about the date and scope of
delivery in case of future queries, re-orders or service work.
Please be sure to remove all small parts from the packaging material.
We wish to inform you that our devices are adjusted at the factory and can be used
immediately after installation.
Before using the RIDA®CYCLER for the first time, please read the operating instruction
carefully. We recommend that it always be kept with the RIDA®CYCLER.
Important information
Before using the instrument, it is important to read this user manual to familiarize yourself with
the instrument. Follow all instructions to ensure proper operation of the RIDA®CYCLER
instrument. Do not use any consumables, accessories, or external equipment other than that
specified. Safety warnings must be adhered to at all times to avoid risk in personal injury
and/or damage to the instrument. If the equipment is used in a manner not specified by the
manufacturer, the protection provided by the equipment may be impaired. The advice given in
this manual is intended to supplement, not supersede, the normal safety requirements
established in the user’s country.
Explanation of symbols used
In these operating instructions, special information is highlighted by the use of symbols:
Warning
Follow the instructions to avoid risk in personal injury
Caution!
Follow the instructions to avoid damage to the instrument.
Warning!
Electrical hazard.
Hot surface!
The temperature of the rotor may be above 40 °C (104 °F).
To avoid injury, do not touch the rotor during a run or 5 minutes following an
aborted run
Biological Hazard!
There is potential for exposure to infections agents when working with
equipment used in molecular biology. To avoid exposure to such hazards,
ensure that proper personal protective equipment is worn and that laboratory
best practise is adhered to.
General information

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ATTENTION
Follow the instructions to ensure optimal instrument performance

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Proper Use Warnings
Warning
Damaged Lid
Do not use the RIDA®CYCLER instrument if the lid is broken or if the lid lock is
damaged. There is a high risk of personal injury to the user through parts that
are moving, electrically live, or are hot.
Caution!
Lethal voltages inside the instrument.
When the instrument is connected to line power, terminals may be live.
Opening covers or removing parts is likely to expose live parts
Warning!
Power Supply Grounding
Power supply must be connected to an outlet with appropriate grounding
means.
Warning!
Main Supply Cord
Do not replace detachable main supply cord with an inadequately rated cord.
Warning!
Do not clean the chamber with flammable liquids.
The chamber can reach temperatures above 100 °C (212 °F). Any flammable
liquids in the chamber could be a fire risk.
Hot Surface!
In the event of a user aborted run do not open the lid until the instrument has
cooled. The rotor within the chamber could be above 40 °C (104 °F). To avoid
personal injury, do not touch the rotor for at least 5 min.
Caution!
Magnetic Tube Clamp
Ensure that the magnetic tube clamp is in place before starting a run to ensure
the caps and tubes do not come out of the wells during the run..
Caution!
Positioning the Instrument.
Do not position the instrument so that it is difficult to operate the disconnecting
device
Caution!
Avoid spilling liquid into the chamber.
Any solution that spills onto electronic boards could cause a short circuit,
damaging the instrument.
Caution!
Do not obstruct the side vents.

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Keep the side vents free from obstruction to prevent interference with the
cooling of the instrument.

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Caution!
Avoid spilling liquid into the chamber.
Any solution that spills onto electronic boards could cause a short circuit,
damaging the instrument.
Caution!
Do not obstruct the side vents.
Keep the side vents free from obstruction to prevent interference with the
cooling of the instrument.
Caution!
Do not move the RIDA®CYCLER instrument during operation.
Movement may impair the proper function of the instrument resulting in poor
data.
Caution!
Power Connection
Avoid removing the power connector from the RIDA®CYCLER instrument
before the power indicator light is off on the power adaptor. Failure to do so
may result in electrical arcing..
Caution!
Authorized Service Only.
There are no user serviceable parts inside the instrument. Service should only
be performed by an authorized party.
Intended use
The RIDA®CYCLER instrument is intended to be used to perform qPCR for molecular biology
research applications that include food and feed and basic life science.
The RIDA®CYCLER instrument is intended for use by laboratory technicians and physicians
trained in molecular biology.
RIDA®CYCLER is intended for Research Use Only.
The RIDA®CYCLER is a compact rotary based 48-well qPCR instrument that applies magnetic
induction to achieve heating and forced airflow for cooling. The instrument comes with four
detection channels with excitation and emission spectra that encompass the most common
dyes used in qPCR.
With Bluetooth® connectivity to a PC, multiple instruments can be run via a single computer
increasing sample throughput. Once multiple runs are generated they can be combined into a
single analysis of up to 10 runs for a total of 480 samples.

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1 Unpacking and installation
The following items are packaged within the RIDA®CYCLER shipping container:
•RIDA®CYCLER instrument (with tube clamp inside)
•Power adaptor
•Power cable
•2 m USB cable
•Bluetooth®antenna
•Capping tool
•Mic tubes and caps (960 reactions)
•USB flash drive containing copy of the software and manual
•RIDA®CYCLER Quick Start Guide
1.1 Hardware installation
Place the RIDA®CYCLER instrument on a level surface.
Screw in the Bluetooth® antenna at the back of the instrument; or connect the instrument to a
PC using the provided 2 m USB cable.
Instrument is not to be used with a USB cable greater than 3 m.
Plug the power cord into the adaptor and insert the adaptor into the back of the instrument.
To avoid electrical arcing, ensure that the power adapter is not plugged into a
wall socket before plugging into the instrument.
Plug the power cord into a wall socket and switch the power on at the socket.
Power the instrument ‘On’ using the power switch at the back of the instrument.
An illuminated blue light at the front of the instrument will show the instrument is powered on.
To switch the instrument ‘Off’, use the power switch at the back of the instrument.
1.2 Software installation
Install the RIDA®CYCLER Software, located on the provided USB Flash drive, onto a PC.
Ensure that the PC meets the following minimum requirements:
•Windows® 7, 32-bit (English version) Operating System
•NET Framework 4.5 or higher
•Intel i5 processor, 2.4 GHz
•4 GB of RAM
•1 GB free hard drive capacity
•Pointer device
•USB Drive
•Adobe®Reader®must be installed to be able to view reports in PDF format.

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•Ensure that the PC has Bluetooth® enabled if this is the preferred connection.

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In the USB Flash drive menu, double click RIDA®CYCLER.msi software installer.
Follow the instructions that appear in the Setup Wizard.
If the computer is connected to a network, network policy settings may prevent you from
completing this procedure. For more information, contact your system administrator.
When the software has been successfully installed, the
RIDA®CYCLER software icon will appear on the PC desk top
Open the RIDA®CYCLER software from the desk top icon.
The software will recognize the instrument via Bluetooth®or USB by displaying the Instrument
icon in the tool bar (top right).
Multiple instruments can be recognized by the software and will be displayed.
Instruments connected to a PC via
Bluetooth®and USB.
Fig. 1: All connected instruments are now ready to be used
1.3 Updating software
Please check the website (www.r-biopharm.com) periodically to see if new software and
firmware updates are available or contact your R-Biopharm sales representative.
Download the RIDA®CYCLER software update setup file.
To initiate the installation, double-click on the setup file and follow the prompts.
The previous version will be uninstalled.
1.4 Upgrading firmware
Some new releases of software will require a firmware update. The software will notify the user
of the requirement to upgrade firmware upon selection of an instrument following the software
update.
To achieve the firmware upgrade, the instrument must be connected to the PC via USB cable.
Firmware upgrades cannot be achieved through Bluetooth®.
Select the Upgrade Firmware option in the drop-down list after selecting the Instrument icon.
The instrument will begin to flash the front LED indicator red to notify the user the firmware is
being updated.

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2 RIDA®CYCLER: Overview
1 Rotor -
Aluminum rotor with 48 sample
positions and a tube location label
to ensure correct loading of tubes.
2 Tube clamp -
Safeguards against the tubes and
caps from coming out of the rotor
during a run.
3 Lid Lock -
Mechanism that locks the lid shut
during a run, to protect the user
from moving parts.
Fig. 2: RIDA®CYCLER Top view
4 Lid –
Provides access to the rotor.
5 LED indicator –
When illuminated blue, indicates that the
instrument is powered ‘On’ or green, the
instrument is ‘Running’.
6 Extraction fan - Ensures cooling of
electronics.
7 Power switch - Powers the instrument
on/off.
8 USB cable inlet - USB connection to a PC.
9 Bluetooth® antenna - Wireless connection
to a PC.
10 Power inlet - Connects to the power
adaptor.
Fig. 3: RIDA®CYCLER Front and back view
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3 Consumables and Accessories
Power adaptor
(Art.No. ZRC-MIC-PA)
External power supply for the
instrument.
Provided with instrument.
Tubes and caps
(Art.No. ZRC-MIC-
TUBES)
Strip of four reaction vessels with a
volume range of 5 - 30 µL.
Preloaded with silicone oil.
Pre-packaged into a rack of 48
tubes, stacked together in a row of
5, and boxed as 4 x 5 stacks.
One box provided with instrument.
Capping tool
(Art.No. ZRC-MIC-CT)
Allows for easy insertion of the
caps into the tubes.
Provided with instrument.
Mic SBS robotic loading
block
(Art.No. ZRC-MIC-
SBSLB)
SBS dimension loading block to fit
most liquid handling systems.
Loads 2 x 48 well Mic racks.
Fig. 4: Consumables and accessories
4 LED Indicator Colours
The LED indictor will change color and flash during particular instrument operations.
Blue constant:
The instrument is switched on and Idle.
Blue flashing:
The instrument has been selected to Start a run. This instrument can no
longer be selected by another user until the designated run has completed.
Green:
The instrument is Running.
Green flashing:
The instrument has completed the run successfully.
Red flashing:
The run has been Aborted, the instrument has had an issue during the run,
or the firmware is being upgrade.

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5 Getting started
5.1 Loading tubes
Each tube is part of a strip of four, with the first tube having a small tab to ensure the strip is
loaded into the instrument correctly. Each tube is preloaded with silicone oil, which acts as a
barrier to prevent evaporation and condensation, thereby improving reaction performance
and removing the need for a heated lid. The allowable range of total reaction volume is 5 - 30
µL.
Side View Front View
Top View Caps
Tube
Tab
The caps are designed to fit tightly
into the tubes. Each tube is
preloaded with silicone oil that acts
as an overlay during the reaction,
limiting condensation.
A tab on tube one is used as a
marker to ensure the tubes are
correctly oriented into the loading
block and instrument.
Fig. 5: Closing the tubes
Use the provided loading rack to pipette reagents and samples into each tube.
The tubes are pre-packed into a loading rack, with the tube tab matching the tab on the loading
rack.
The loading rack is also compatible with a multichannel pipette (8-channel). For this strategy,
the loading orientation switches to across the top of the loading block (A1, A2, A3…) instead of
the standard down orientation (A1, B1, C1…). The software has provision to change the display
of samples based on the type of layout selected.
Note: Ensure that only the first six tip positions are utilized and not the full eight.
Wells highlighted in green
follow the standard loading
orientation (A1, B1, C1…)
and
wells highlighted in blue
follow the multi-channel
pipette loading orientation
(A1, A2, A3…).
The loading strategy will
allow you to load half a 96-
well plate per loading block

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Fig. 6: Loading rack set-up