Rokit Dr. INVIVO 4D User manual

Changing the world by serving patients through
personalized regenerative therapeutics
Version 2020-01-15

Confidential & Proprietary|© ROKIT Healthcare Inc. 2019. All Rights Reserved
Table of Contents
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Confidential & Proprietary|© ROKIT Healthcare Inc. 2019. All Rights Reserved 3
The product, software or services are being provided on an “as is” and “as available” basis. Except as may be stated specifically in your
contract, ROKIT Healthcare expressly disclaims all warranties of any kind, whether express or implied, including, but not limited to, any
implied warranties of merchantability, fitness for a particular purpose and non-infringement. You understand and agree that ROKIT
Healthcare shall not be liable for any direct, indirect, incidental, special, consequential or exemplary damages, including but not limited to,
damages for loss of profits, goodwill, use, data or other intangible losses (even if ROKIT Healthcare has been advised of the possibility of
such damages), resulting from:
(i) the use or the inability to use the product or software;
(ii) the cost of procurement of substitute goods and services resulting from any products, goods, data, software, information or services
purchased;
(iii) unauthorized access to or alteration of your products, software or data;
(iv) statements or conduct of any third party; (v) any other matter relating to the product, software, or services.
The text and drawings herein are for illustration and reference only. The specifications on which they are based are subject to change.
ROKIT Healthcare may, at any time and without notice, make changes to this document. ROKIT Healthcare, for itself and on behalf of its
subsidiaries, assumes no liability for technical or editorial errors or omissions made herein, and shall not be liable for incidental,
consequential, indirect, or special damages, including, without limitation, loss of use, loss or alteration of data, delays, or lost profits or
savings arising from the use of this document.
The content, organization, graphics, design, compilation, magnetic translation, digital conversion and other matters related to the
information in this document are protected under applicable copyrights, trademarks and other proprietary (including but not limited to
intellectual property) rights. The copying, redistribution, use, reuse or publication of any part of the document without advance notice to
and approval from ROKIT Healthcare is strictly prohibited.
INV-CHS-20191210

Confidential & Proprietary|© ROKIT Healthcare Inc. 2019. All Rights Reserved
Safety Caution
The information below is meant to keep users safe and to protect equipment against damage. Please read through carefully.
The following instructions prevent safety hazards that could potentially result in death and serious personal injury.
The following instructions prevent safety hazards that could potentially result in equipment damage.
Keep the equipment out of reach of children and infants.
Keep children away from wrapping a power chord around the neck as it can cause choking.
Do not let children turn on or touch the equipment, which may cause a safety accident.
Use the recommended power source only.
Inappropriate use of electricity may cause equipment failure, electric shock or fire.
Use an outlet that matches the electrical capacity of the equipment provided (including any accessory equipment).
If multiple devices are provided, connect to a separate outlet for each device.
Do not install near any hot air or heat-prone items, places exposed to moisture, oil, dust, direct sunlight, water (rain, etc.), or gas leaks or
flammable volatiles.
The above may result in electric shock, fire, equipment failure, or explosion.
Install in a place with a strong and level floor. Please make sure that the equipment sits fully in contact with the floor.
Otherwise, it may cause abnormal vibration, noise and malfunction of the equipment.
Install with the guide of a trained professional technician.
Incorrect installation may cause an electric shock, fire, malfunction, explosion or injury.
Keep eyes and hands away from the door while the UV lamps are on. This may cause eye injury and burn.
Installation
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CAUTION
WARNING
INV-CHS-20191210
WARNING

Confidential & Proprietary|© ROKIT Healthcare Inc. 2019. All Rights Reserved
Safety Caution
While using Dr. INVIVO
Do not put hands or any part of the body inside the equipment while printing is in progress.
Do not put hands or any part of the body inside the equipment when there has been a strong impact or damage to the equipment.
It may result in serious injury.
Malfunctions in the equipment can cause personal injury.
If smoke, strange odor, or abnormal operation occur, stop the equipment immediately.
Do not use organic solvents such as benzene, thinner, etc. to clean the equipment. If you clean with alcohol, please use the equipment after
alcohol has been completely dried.
Do not spray flammable materials such as paint, insecticide, or fragrance on the equipment.
Do not use or store temperature-sensitive equipments such as flammable sprays, burning materials, dry ice, medicines, or academic
materials near the equipment. Also, do not store volatile or flammable objects or materials (benzene, thinner, propane gas, etc.) or electrical
appliances.
Do not allow water, other liquids, or foreign objects to enter the equipment.
If water, other liquids, or foreign objects get inside, it may cause equipment failure, electric shock or fire.
If water, other liquids, or foreign objects enter the equipment, please contact our service center.
Do not touch the equipment or power plug during rainstorms.
It may cause electric shock or fire. Stop operating the equipment and keep away from it.
Do not touch a power cord with wet hands. It may cause electric shock.
Keep water, soil, or metallic objects away from the plug.
Prevent the power plug from damage.
Do not use any damaged power plug, power cord, or a loose outlet.
Important: Never disassemble, repair or modify any parts or whole of the equipment without advance notice to the service center.
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WARNING
INV-CHS-20191210

Confidential & Proprietary|© ROKIT Healthcare Inc. 2019. All Rights Reserved
Caution
While using Dr. INVIVO
Disconnect the power plug when cleaning the equipment.
It may cause electric shock or fire.
Do not spray water or wipe with benzene, thinner, etc.
It may cause electric shock or fire due to short circuit.
The equipment may discolor or peel off the coating.
Do not wipe with a wet cloth, etc.
It may cause electric shock or fire due to short circuit.
After cleaning, check that the power cord is not damaged, that there is no heat generation on the power plug, and that the
plug is plugged in properly.
Avoid using it under the direct sunlight, high heat, or cold temperature.
Avoid using in places with high vibration.
Do not push or tilt the equipment with excessive force.
It may cause personal injury or damage the floor.
The coolants supplied are a chemical. Please read the instructions carefully and use them correctly.
Be sure to also read the instruction manuals of the supplied Air Compressor and air dispenser controller.
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CAUTION
INV-CHS-20191210

Confidential & Proprietary|© ROKIT Healthcare Inc. 2019. All Rights Reserved
Caution
While using Dr. INVIVO
Cautions when using the UV lamp:
Do not look into the UV light while it is in operation. There is a risk of eye injury and burn.
Wear protective glasses when operating with the UV lamp on.
The UV lamp is turned on / off using the red switch at the top right of the equipment.
Turn off the UV lamp when not using the equipment.
The recommended replacement cycle of the UV lamp is 14 months (based on 24 hours of use).
Cautions when using the HEPA filter:
Turn on / off the HEPA filter with the green switch on the upper right side of the equipment.
Turn off the UV lamp and start the HEPA filter before starting to use the equipment.
The recommended replacement cycle of the HEPA filter is 8 months. (※ The replacement cycle varies depending on the
environment.)
When using the equipment inside a biosafety cabinet, it is okay to turn off the HEPA filter.
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CAUTION
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Confidential & Proprietary|© ROKIT Healthcare Inc. 2019. All Rights Reserved
Dr. INVIVO 4D Overview
With every revolutionary and disruptive technology, medicine takes a leap forward to improve patient health and quality of life.
For long, 3D bioprinting of cells and tissues has stayed on the research bench, but at ROKIT Healthcare, we’re working to
move it into the Operating Theater.
Look ahead beyond research and set your sights on what this technology can do to make a difference in healthcare.
The promise of an automated, customized, and point-of-care patient solution of the future is in your hands.
INVIVO is the world’s first clean-bench hybrid 4D biofabricator capable of printing biologically active structures with materials
ranging from cells and hydrogels to thermoplastic filaments, pellets, and slurries.
INVIVO offers:
•Wide range of material types, including cells, hydrogels, polymeric filaments, pellets and slurry
•Versatile three printhead technologies with the widest control of temperature range:
•Syringe bio-dispenser (-4 ℃to 60 ℃)
•Filament extruder (RT to 250 ℃)
•Hot-melt pneumatic dispenser (RT to 350 ℃)
•Printbed (-4 C to 60 ℃)
•Customizable UV LED lamps for photocrosslinking at various wavelengths
•Clean-chamber environment with H14-grade HEPA filter and 254nm UV lamps
•Dark chamber environment for photosensitive materials
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INV-CHS-20191210

Confidential & Proprietary|© ROKIT Healthcare Inc. 2019. All Rights Reserved
Dr. INVIVO 4D Overview
Technical Specifications
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Confidential & Proprietary|© ROKIT Healthcare Inc. 2019. All Rights Reserved
Dr. INVIVO 4D Overview
CATEGORY STANDARD UPGRADE PREMIUM
Printing Modules Filament Extruder +
Syringe Bio-Dispenser Filament Extruder + Syringe Bio-Dispenser
Filament Extruder + Syringe Bio-
Dispenser
+ Hot-Melt Pneumatic Dispenser
LCD LCD Touch Screen / Android OS
Printing Degree of
Precision Filament Extruder 0.2mm / Syringe Bio-Dispenser 0.08mm
HEPA Filter H14 HEPA Filter
Disinfecting UV Lamp Disinfecting UV Lamp (12W/254nm)
UV LED UV LED (365nm)
Extruder Removable extruder nozzle / tube system
Syringe Bio-Dispenser Mechanical precision motor syringe (10 ml default; 3 ml optional)
Curing UV Lamp - UV LED (365 nm) UV LED (365 nm)
Syringe Bio-Dispenser
Temperature Control -Temperature control function (- 4~60℃) Temperature control function (- 4~60℃)
Printing Bed
Temperature Control -Temperature control function (- 4~60℃) Temperature control function (- 4~60℃)
Nozzles Extruder: Ø 0.2~0.4mm
Syringe Bio-Dispenser: Ø 0.1~0.4mm Extruder: Ø 0.2~0.4mm Syringe Bio-Dispenser: Ø 0.1~0.4mm
Extruder: Ø 0.2~0.4mm
Syringe Bio-Dispenser: Ø 0.1~0.4mm
Hot-Melting Pneumatic Dispenser
: Ø
0.1~0.5mm
Hot Melting Pneumatic
Dispenser - - Hot-Melting Pneumatic Dispenser (~350℃)
Build Volume 100 X 100 X 80mm
Printing Speed 3~20mm/sec
※ NOTE : The above specifications may be subject to change without notice to improve performance
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SPECIFICATION
INV-CHS-20191210

Confidential & Proprietary|© ROKIT Healthcare Inc. 2019. All Rights Reserved
Dr. INVIVO 4D Overview
Basic Components
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※ The components above are subject to change without notice to improve performance.
※ Of the above components, syringes and syringe tips are for in vitro research only and cannot be used on animals or humans.
CAUTION
WARNING
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1 Bio Dispenser 1 ea
2 Filament Extruder 2 ea
3 Filament Guide Tube 1 ea
4 Filament Holder 1 ea
5 Pin 1.8mm 1 ea
6 Niddle 0.18mm 1 pack
7 Hex Wrench (1.5, 2.0, 2.5, 3) 1 set
8 Allen Bolt 4x45 6 ea
9 Countersunk Bolt 4x12 5 ea
10 Thickness Gauge 1 ea
11 Syringe 10ml 5 ea
12 Syringe Tip 6 ea
13 Taper Tip 6 ea
14 Petri Dish 1 pack
15 Dongle (Wireless LAN) 1 ea
16 Leveling Jig 1 ea
17 Screwdriver 1 ea
18 Water Drip 2 ea premium
19 Glass Plate 1 ea
20 PLA Filament 1 ea
21 Power Cable (INVIVO Body) 1 ea
22 USB Cable 1 ea
23 Component Bag 1 ea

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Dr. INVIVO 4D Overview
12
※ The components above are subject to change without notice to improve performance.
Accessory Components
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1 Precision Nozzle 0.2mm 1 ea premium
2 Barrel 1 ea premium
3 Adapter 1 ea premium
1 Air Tube(cto5) 1 ea premium
2 Air Tube(cto6) 1 ea premium
3 Connecting Cable 1 ea premium
4 Rubber O-ring 1 ea premium
5 Power Cable(Air Dispensor Controller) 1 ea premium
6 Air Tube 1 ea premium
7 Coupler 1 ea premium
1 Power Cable(Temperature Controller) 1 ea premium
2 Invivo Liquid(Coolant) 6 ea premium
3 Rubber Hose 2 ea premium
4 Clamp 4 ea premium
5 Silicone Cover 1 ea premium
6 Valve Fitting 4 ea premium
7 Rubber O-ring 1 ea premium
Pneumatic Dispenser
Air Dispenser Controller
Temporature Controller

Confidential & Proprietary|© ROKIT Healthcare Inc. 2019. All Rights Reserved
Dr. INVIVO 4D Installation
Preparation Before Installation
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In order to prevent injuries from lifting heavy loads, work in a group of at least two persons to hold or move the equipment
and follow the below guidelines:
- Install the equipment on a flat and solid floor.
Uneven floors can cause vibration and noise, as well as a probable increase of slants for bed leveling and reduction of print
quality.
The floor should be sturdy to withstand the weight of the equipment.
- Install the equipment with some space for ventilation.
Be sure to keep the back side at least 5 cm from the walls for good ventilation.
- Avoid places with direct sunlight, high heat or excessive cold.
Install at an ambient temperature, typically between 5 ℃ and 35 ℃.
If the temperature at the installation place is too high or too low, the heating or cooling function may malfunction and the
print quality may be reduced.
INV-CHS-20191210
Component Width x Depth x Height (mm) Weight (kg) Memo
Invivo Body 690 x 690 x 990 48
Temperature Controller 520 x 480 x 365 16.5
Air Compressor 820 x 550 x 560 49
Air Dispenser Controller 480 x 440 x 205 7
[Packaging specification ]

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1. Unbox and place the Dr. INVIVO body on a level and unclustered surface.
2. Remove the shock absorber and cable ties inside of the equipment.
3. Fully insert the power plug into a grounded power outlet.
4. Turn on the power.
5. When the system boots, an Android logo appears. If operational, the NewCreatorK app would turn up on the LCD screen.
6. Turn off the power. Remember to keep the system turned off during installation and connection of accessory equipments
to the body.
Dr. INVIVO 4D Installation
Before removing package After removing package Power on NewCreatorK on Power off
Installation of INVIVO Body
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INV-CHS-20191210
[Packaging specification
690 x 690 x 990, 48 kg]

Confidential & Proprietary|© ROKIT Healthcare Inc. 2019. All Rights Reserved
Dr. INVIVO 4D Installation
1. Unbox and place the Temperature Controller on a level and unclustered surface.
2. After placing each provided metal clamp on either end of the rubber hoses, connect the hoses between the Temperature Controller and the
Dr. INVIVO main body.
3. Clamp the rubber hoses and valves securely (to prevent leakage of the coolant).
4. Open the valves at the Temperature Controller and the Dr. INVIVO body.
5. Lift the silicone cover of the coolant inlet of the tank and pour 5 bottles of the coolant supplied with Dr. INVIVO (6 bottles are supplied in
total)
6. Close the coolant inlet with the silicone cover.
7. Insert the power plug into a grounded power outlet. Turn on the power switch of the Temperature Controller. Make sure that the cable is
not yet connected between the Temperature Controller and the Dr. INVIVO body.
8. Adjust the set temperature of the Temperature Controller to room temperature (20 ~ 25 ℃).
9. Circulate the coolant for 3 to 4 minutes until there are no bubbles in the hose.
10. After the coolant circulates, check the inside of the tank and add coolant if the level has dropped.
11. Turn off the power of the Temperature Controller.
12. Align the groove of temperature control cable with the one in the socket and connect it gently. Do not use excessive force.
13. Turn on the body and the Temperature Controller together. Operate the Temperature Controller to verify that the bed and syringe
temperature are adjustable. First test at -1 ℃, then test at 25 ℃.
14. Turn off the power and end the installation.
1. Make sure to follow the above instructions step by step. Only after the installation of the Temperature Controller is complete and the coolant has
been circulated sufficiently in the Dr. INVIVO body, turn on the Dr. INVIVO body.
2. When connecting the electric system cable, connect only when the power is off.
3. The coolant supplied with Dr. INVIVO is a chemical. Be sure to read the precautions on the container surface and use them correctly.
Installation of Temperature Controller (PREMIUM & UPGRADE)[Packaging specification 520x480x365, 16.5 kg ]
1. Use the original coolant supplied with Dr. INVIVO.
2. The coolant will evaporate over time, so check regularly and refill.
CAUTION
WARNING
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INV-CHS-20191210

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Dr. INVIVO 4D Installation
16
Circulate
the
coolants
(3~4 min)
Power off
※ When installing, connecting and disconnecting
any parts or equipment, power off the system
first.
1 1 2 2,3 2,3
4 5 6 7
8 9 11 1010
13
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WARNING
INV-CHS-20191210
[520x480x365, 16.5 kg ]

Confidential & Proprietary|© ROKIT Healthcare Inc. 2019. All Rights Reserved
Dr. INVIVO 4D Installation
1. Unbox and place the Air Compressor on a level and unclustered surface. Turn the red emergency button clockwise and
unlock for operation.
2. Connect the air tube 1 to the Air Compressor and Air Dispenser Controller keeping the air-in and air-out sockets aligned.
Insert the tube completely until it clicks.
3. Insert the power plug into the power outlet.
4. Turn on the power. If the equipment does not operate, check again the red emergency button.
5. Check for a pressure rise. The gauge on the left shows the tank pressure and the gauge on the right shows the regulator
pressure.
6. The Air Compressor will stop the pressure rise automatically when the pressure gauge has reached ~ 9 bar. If the pressure
does not rise, turn the pressure regulator clockwise until you see the gauge needle move.
7. Turn the regulator to adjust to the required pressure.
8. Turn off the power and finish the installation.
1. Before installing or using the Air Compressor, be sure to read the operating instructions provided with Dr. INVIVO.
2. When not in use for a long time, remove the air using the vacuum control knob on the Air Dispenser Controller.
Installation of Air Compressor (Premium) [Packaging specification 820 x 550 x 560, 49 kg ]
1. When using the Air Compressor, the mode of Air Dispenser should be set to STEADY.
2. If the vacuum of the Air Dispenser Controller is open, an air leak will be heard and the air pressure will not rise. Lock the vacuum.
3. Drain the water from the water filter before using the Air Compressor.
4. Check the drain bucket on the back of the Air Compressor at least once a month and empty it if it is full.
5. Replace the air dryer every 6 months.
6. Check and replace other consumable parts.
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CAUTION
WARNING
INV-CHS-20191210

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Dr. INVIVO 4D Installation
18
Installation of Air Compressor (Premium) [Packaging specification 820 x 550 x 560, 49 kg ]
1 1
4
2
3
※ When installing, connecting and disconnecting any parts or equipment, power off the system first.
CAUTION
INV-CHS-20191210
[Packaging specification 820 x 550 x 560, 49 kg ]

Confidential & Proprietary|© ROKIT Healthcare Inc. 2019. All Rights Reserved
Dr. INVIVO 4D Installation
1. Unbox and place the Air Dispenser Controller on a level and unclustered surface.
2. Turn off the power of Dr. INVIVO.
3. Connect the air tube 2 to the Air Dispenser Controller and Dr. INVIVO body.
4. After confirming that the power of the Dr. INVIVO body is turned off, connect the connecting cable to the Air Dispenser
Controller and the Dr. INVIVO body.
5. Connect the air tube3 to the Dr. INVIVO and hot-melting block.
6. After confirming that the power of Dr. INVIVO is turned off, connect the hot melt control connector.
7. Insert the power cord plug into the power socket.
8. Turn on the power of the Air Dispenser Controller.
9. Check that the Air Dispenser LCD screen is on.
10. Check the change of pressure on the Air Compressor.
11. Check that the air pressure of the Air Compressor can be adjusted by the Air Dispenser Controller.
12. Make sure the temperature of the Air Dispenser can be adjusted by the Air Dispenser Controller.
13. Check that the vacuum is working.
14. Turn off and end installation.
1. Turn off the vacuum valve before use
Installation of Air Dispenser Controller (Premium) [Packaging specification 480 x 440 x 205, 7 kg ]
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CAUTION
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Dr. INVIVO 4D Installation
20
Installation of Air Dispenser Controller (Premium) [Packaging specification 480 x 440 x 205, 7 kg ]
1 1 2
4 4 6 7
8
2
※ When installing, connecting and disconnecting any parts or equipment, power off the system first.
CAUTION
INV-CHS-20191210