Roth LuciPac A3 Aqua User manual

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LuciPac®A3 Aqua
From Kikkoman
For analysis of contamination of liquids via ATP/ADP/AMP measuring by Lumitester
PD30/Smart, e.g. for contamination monitoring of waters and waste waters.
1. Introduction
Using the LuciPac®A3 Aqua sampling pen, liquids can be tested quickly and easily for organic
contamination. The organic contamination of liquids, e.g. by bacteria, is indicated by the presence
of ATP/ADP/AMP in these samples. An enzyme/substrate complex in the LuciPac®A3 Aqua
sampling tube reacts with ATP/ADP/AMP to produce bioluminescence whose intensity correlates
directly with the amount of ATP/ADP/AMP present. The Lumitester PD30 and Lumitester Smart
(1E37.1) then determine the bioluminescence as a direct measure of original contamination. The
sampling comb ensures precise sampling of 150 μl with a deviation of ≤2 %. Test results are,
therefore, highly reproducible and comparable.
The unit is easy to operate, even by non-professionals. Just immerse the sampling comb into the
liquid. Then put the comb back into the tube and push it down into the reaction chamber. Shake
the tube until the reagents are released and measure the bioluminescence in the Lumitester PD30
or Lumitester Smart.
2. Shipment and storage
20 LuciPac®A3 Aqua each are packed in a resealable aluminium bag. Kits are to be stored at a
low temperature (2 °C to 8 °C (35.6 °F to 46.4 °F)) for long term storage. The kit can be stored
below 25 °C (77 °F) for up to 14 days or below 30 °C (86 °F) for up to 5 days before opening an
aluminum bag without any adverse effect on the long term stability. Do not freeze the device.
We recommend that you use all 20 sampling devices in a single bag at one time after opening an
aluminum bag. If you have leftover sampling devices that you must store after opening a bag, be
sure to store them at the recommended low temperature (2 °C to 8 °C (35.6 °F to 46.4 °F)) and
use them within two weeks after opening.
Expiry date: printed on the label of the aluminum bag. Store protected from light.
3. Comments on application, detection limits
The sampling stick (comb) of this device can collect up to 0.15 ml of water and ATP/ADP/AMP can
be measured from 10-11 mol/l to 10-6 mol/l using LuciPac®A3 Aqua combined with Lumitester PD30
or Lumitester Smart (1E37.1). Therefore, the minimum measurable level of bacteria would
correspond to about 104cells/ml calculated from said ATP content of 10-18 mol in a single
bacterium. Please be aware that you cannot detect very low concentration of bacteria by this
method.
For detection of very low amounts of bacteria, membrane filtering methods should be applied.
Please filter some bigger amount of liquid (typically 100 ml) through a membrane filter.
Subsequently, probe this filter with LuciPac®A3 (1ENH.1) and measure using the PD30 or
Lumitester Smart (1E37.1).
Instructions for use

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This kit can be used to test cleanliness levels of water and other liquid samples. Do not use this
device for any purpose other than what it was designed for as a tester of cleanliness levels of
water, other liquid samples, or other application which is instructed to use by the manufacturer.
Please be aware that this kit cannot be used to test or identify specific types of pathogenic
bacteria. Water and other liquid samples may contain ATP/ADP/AMP which are caused by
improper cleaning of facilities or originate from microorganisms propagating in such samples.
Since this device measures ATP/ADP/AMP levels immediately, indications of improper cleaning
processes, e.g. inadequate microbiological control of coolant or other industrial process water can
be promptly detected. This device shall not provide any guaranty that a given test sample is free of
bacterial contamination. Thus it is not suitable for microbial testing of originally ATP rich
beverages, such as fruit juices.
4. Mechanism
This test system uses a cyclic method based on a combination of firefly luciferase, pyruvate
orthophosphate dikinase (PPDK) and a pyruvate kinase (PK). The method produces a defined
amount of luminescence proportional to the amount of adenosine triphosphate (ATP), adenosine
diphosphate (ADP) and adenosine monophosphate (AMP) in a sample.
Firefly luciferase emits light in the presence of ATP and luciferin. The AMP generated from this
reaction is converted back to ATP using the PPDK, while existing ADP is converted to ATP by the
pyruvate kinase (PK) to produce a luminescent signal that is as good and stable as possible.
ATP is a source of energy necessary for various forms of life that is present in organic residues,
such as microorganisms, food residues, and biological substances that originate from other living
organisms. This method allows you to measure and detect organic residues and micro-organisms
at high speed and high sensitivity by detecting ATP using luciferase, to monitor cleaning processes
and microbiological control of coolant or other industrially processed water.
Furthermore, you can use this technique to determine not only ATP, but also ADP and AMP. AMP
is formed from ATP in bacteria when they are in a resting state and shut down the energy balance.
ADP and AMP are derived from ATP during processing, such as heat treatment or fermentation.

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5. Composition of product
6.
6. Please note
Please make sure to follow the instructions outlined below in order to obtain optimal performance
from this device:
1. Do not use products with a shelf life that has already expired. Expired products may not yield
accurate results (the expiry date is printed on the label of the aluminum bag holding the
sampling devices).
2. Be sure to use only designated products, i.e. type of Lumitester when performing luminescent
measurements. This device cannot be used with unqualified products.
3. Prior to testing the sampling devices should be allowed about 20 min to reach room
temperature (20 °C to 35 °C (68 °F to 95 °F)), if they are from a refrigerated stock.
Measurement values may deviate from the real value, if the swabs are used without
equilibration. Use the sampling devices as soon as possible once they are available at room
temperature. Do not keep the device at temperatures exceeding 35 °C (95 °F). High
temperatures may cause product performance to drop.
4. Wherever possible, be sure to use up all sampling devices from a single bag that has been
opened at one time. However, if you must store leftover sampling devices once finished with a
test session, firmly close the aluminum bag and store it in a refrigerated environment (2 °C to
8 °C (35.6 °F to 46.4 °F)). High temperatures may cause product performance to drop.
5. Do not subject the device or any part of it to direct sunlight for long periods of time. Strong light
may cause product performance to drop.
6. Do not touch any of the parts inside the device, particularly not any part of the sampling stick
(comb) itself with a finger or other object before use. Touching the parts may affect cleanliness
levels, making them hard to discriminate.
7. Do not drop the device or any of its parts or allow any parts to be struck or jolted. The interior
aluminum sheets and other parts in the kit may become damaged, causing product
performance to drop.
8. Do not use the device if any parts become damaged, e.g. the inner aluminum sheets
separating the reagent chamber from the comb as well as the luminescent reagent. Such
damage will affect product performance. The aluminum sheet is not damaged if the releasing
reagent stays in the chamber upon agitation.
Reagent cocktail:
Luciferin, luciferase, phosphoenolpyruvate (PEP),
magnesium acetate, pyrophosphopyruvate
pyruvate/phosphate dikinase
Releasing reagent:
Alkyl dimethylbenzylammoniumchloride
Structural parts Raw material
Stick holder (green)
Polypropylene
Test tube (body)
Sampling comb (red)
ABS Resin
Container for releasing reagent
Polypropylene,
aluminium
Measurement tube
Swab holder (green)
Sampling stick (red)
Luminescent reagent
Test tube
Container for releasing
reagent

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9. Do not soak more than 3 cm from the tip of the sampling stick (comb) into water or other liquid
samples. Water or other liquid samples which wet the shaft of the sampling stick (green) may
affect test results.
10. Ensure that the LuciPac®A3 Aqua is not shifted or displaced, discard the item, because
accuracy cannot be guaranteed, swabs are not removable from the Lumitester PD30/Smart,
or the instrument may cause an error (malfunction).
11. If the test reagent is leaking please don't use the swab!
7. Measurement
Complete the procedures listed below within a temperature range of 20 °C to 35 °C (68 °F to
95 °F). Make sure to always run measurement tests at the same temperature to maintain
repeatability for comparison. Remove the LuciPac®A3 Aqua from refrigerator and wait until they
have reached room temperature (about 20 min, 20 °C to 35 °C (68 °F to 95 °F)). Use the sampling
devices then as soon as possible.
1. Remove the sampling stick from the main body (casing).
2. Soak sampling stick about 3 cm from the tip of the sampling stick into water or other liquid
samples, and shake gently. Make sure no bubbles remain within sampling stick's comb.
3. Pull out the sampling stick slowly and straight up from the sample.
4. Return the sampling stick to the main body (casing) and push it all the way into the main body.
5. Push firmly onto LuciPac®A3 Aqua casing and agitate.
6. Allow the leftover luminescent reagent to thoroughly dissolve.
7. Insert the LuciPac®A3 Aqua into the Lumitester to measure the results. Press ENTER.
8. Remove the LuciPac®A3 Aqua case from the measuring chamber.
8. Safety recommendations
Please observe the following items to ensure safe use of this product.
Please note safety recommendations given in the Safety Data Sheet!
Do not attempt to drink the kit reagent or touch it with bare hands or allow it to splash into
eyes. Please make sure to read the precautions and instructions in this Instruction Manual
before attempting to use the kit and exercise extreme caution when using it.
Be careful not to get fingers caught when inserting the sampling stick into the main body
(casing).
Handle with care when storing and disposing of the device and its reagents to ensure that
none of the substances become mixed with food and other products.
Please make sure to store this kit and its parts out of the reach of young children.
Take precaution when disposing of this kit after use to ensure that such substances do not
become mixed with food products at food production centers and similar facilities
9. Short instruction
Prior to use, make sure the LuciPac®A3 Aqua sticks have come to room temperature.
But then use immediately.

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LuciPac®A3 Aqua 20 pieces 1ENK.1
100 pieces 1ENK.2
Carl Roth GmbH + Co. KG
Schoemperlenstraße 3-5 • 76185 Karlsruhe • P.O. Box 100121 • 76231 Karlsruhe
Phone: [phone] • Fax: [phone] • [e-mail] • www.carlroth.com
The company is a limited partnership with headquarters in Karlsruhe, reg. court Mannheim HRA 100055. Roth Chemie GmbH, with headquarters in Karlsruhe,
reg. court Mannheim HRB 100428, is the personally liable partner. Managing Director: André Houdelet. Sales tax identification number: DE 143621073. sse 07/2021