RR Mechatronics Lorrca MaxSis User manual

User Manual
Lorrca
Laser-assisted Optical Rotational Cell Analyzer
Version 5.08 (24-04-2023) MRN-231-EN

Contents
1 - Document history overview. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11
2 - About RR Mechatronics. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 13
3 - Introduction. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 14
3.1 - - About this manual. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 14
3.2 - - Typographical conventions. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 14
3.3 - - Used symbols. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 15
4 - Instrument description. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 17
4.1 - - Technical specifications. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 19
4.2 - - Introduction Lorrca® MaxSis. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 22
4.2.1 - - Intended use. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 23
4.2.2 - - Features of the Lorrca Maxsis . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 23
4.2.3 - - Symbols. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 23
4.2.4 - - General measurements description. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 26
4.2.4.1 - - Principle of operation Lorrca Maxsis. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 26
4.2.4.2 - - Introduction Osmoscan. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 28
4.2.4.3 - - Introduction RBC-elongation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 28
4.2.4.4 - - Introduction RBC-aggregation. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 28
4.2.4.5 - - Introduction Oxygenscan. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 28
5 - Safety. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 30
5.1 - - General safety rules . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 30
5.2 - - Electrical safety. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 31
5.3 - - Laser and mechanical safety. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 32
5.4 - - Bio Safety bob assembly. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 33
5.5 - - Safety for working with compressed Nitrogen. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 33
5.6 - - Bio safety Standard Operating Procedures (S.O.P.). . . . . . . . . . . . . . . . . . . . . . . . . . . . 35
5.6.1 - - Basics of bio safety. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 35
5.6.2 - - S.O.P. for working with bio hazardous materials. . . . . . . . . . . . . . . . . . . . . . . . . . 35
Contents
MRN-231-EN Version 5.08 (24-04-2023) 3

5.6.2.1 - - Facts and definitions:. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 35
5.6.2.2 - - Medical requirements:. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 36
5.6.2.3 - - General laboratory practices:. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 36
5.6.2.4 - - Specific laboratory practices and requirements:. . . . . . . . . . . . . . . . . . . . . . 36
6 - Installation. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 38
6.1 - - Pre-installation requirements. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 38
6.2 - - Transport. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 41
6.3 - - Environmental conditions and setting up the instrument. . . . . . . . . . . . . . . . . . . . . . . . 41
6.3.1 - - Conditions. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 41
6.3.2 - - Main power connection. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 41
6.3.3 - - Unpacking / Setting Up. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 42
6.3.4 - - Lorrca to laptop connections. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 44
6.4 - - First Start-up. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 46
6.5 - - Checking Lorrca Maxsis functionality. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 50
6.5.1 - - Instrument status. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 50
6.5.2 - - Lorrca IO. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 51
6.5.3 - - Camera. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 52
7 - Lorrca Maxsis Program. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 54
7.1 - - Main screen. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 56
7.2 - - Main screen settings. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 57
7.2.1 - - Screen communication settings. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 57
7.2.2 - - Hardware settings. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 58
7.2.3 - - End of the day wash screen. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 60
7.3 - - Hardware check status (Lorrca status). . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 61
7.3.1 - - Hardware check IO screen (Lorrca IO). . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 62
7.3.2 - - Hardware check Camera screen. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 63
7.3.3 - - Hardware pO2. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 63
7.4 - - Settings screen. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 65
7.4.1 - - Files. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 65
7.4.2 - - General options. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 66
7.4.3 - - Elongation. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 67
7.4.3.1 - - Deformability curve Parameters. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 67
7.4.3.2 - - Stress curve determination Parameters. . . . . . . . . . . . . . . . . . . . . . . . . . . . 67
7.4.3.3 - - Stability test parameters. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 68
Contents
4MRN-231-EN Version 5.08 (24-04-2023)

7.4.4 - - Aggregation. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 68
7.4.4.1 - - Disaggregation options. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 69
7.4.4.2 - - Syllectogram options. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 69
7.4.4.3 - - Iteration parameters setting. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 70
7.4.4.4 - - FSAR options. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 72
7.4.5 - - Osmoscan options. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 73
7.4.6 - - Oxygenscan options. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 73
7.4.6.1 - - pO2 Steps. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 74
7.5 - - Osmoscan screen. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 76
7.5.1 - - Osmoscan result screen. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 77
7.5.1.1 - - Osmoscan parameters explanation. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 77
7.5.1.2 - - Rinse gradiënt Osmoscan screen. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 78
7.5.2 - - Osmoscan curve. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 79
7.6 - - Deformability curve determination screen. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 81
7.6.1 - - Deformability curve result screen. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 82
7.6.1.1 - - Deformability curve determination parameters explanation. . . . . . . . . . . . . . 82
7.6.1.2 - - Rinse cup deformability screen. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 83
7.7 - - Oxygenscan screen. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 84
7.7.1 - - Oxygenscan result screen. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 85
7.7.1.1 - - Oxygenscan parameters explanation. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 85
7.8 - - Stability Curve determination screen. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 86
7.8.1 - - Stability curve determination results screen. . . . . . . . . . . . . . . . . . . . . . . . . . . . . 87
7.8.1.1 - - Stability curve determination parameters explanation. . . . . . . . . . . . . . . . . . 87
7.8.1.2 - - Open Stability curve files. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 88
7.9 - - Cell Membrane stability screen. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 89
7.9.1 - - Deformability results 1 screen. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 90
7.9.2 - - Rinse cup cell membrane screen. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 90
7.9.3 - - Empty cup cell membrane screen . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 91
7.9.4 - - Cell membrane stability results screen. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 92
7.9.5 - - Deformability results 2 screen. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 93
7.9.6 - - At measurement completion. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 93
Contents
MRN-231-EN Version 5.08 (24-04-2023) 5

7.9.6.1 - - Deformability results 1 parameters explanation. . . . . . . . . . . . . . . . . . . . . . . 93
7.9.6.2 - - Stability results parameters explanation. . . . . . . . . . . . . . . . . . . . . . . . . . . . 94
7.9.6.3 - - Deformability results 2 parameters explanation. . . . . . . . . . . . . . . . . . . . . . . 95
7.10 - - RBC aggregation screen. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 96
7.10.1 - - RBC aggregation result screen. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 97
7.10.1.1 - - Aggregation parameters explanation. . . . . . . . . . . . . . . . . . . . . . . . . . . . . 97
7.10.1.2 - - Open Aggregation files. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 100
7.10.1.3 - - Rinse cup RBC Aggregation. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 100
8 - General operation procedure. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 101
8.1 - - Powering up . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 101
8.2 - - Preparations. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 102
8.2.1 - - Lorrca reagents. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 103
8.2.2 - - RBC necessary per test/scan. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 104
8.2.3 - - Estimated number of RBC in the laser path. . . . . . . . . . . . . . . . . . . . . . . . . . . . . 105
8.2.4 - - Oxygenscan whole blood volume calculation. . . . . . . . . . . . . . . . . . . . . . . . . . . . 105
8.3 - - Performing Osmoscan. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 105
8.3.1 - - Preparing a blood sample. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 106
8.3.2 - - Fill in sample ID and viscosity and place sample tube. . . . . . . . . . . . . . . . . . . . . 106
8.3.3 - - Adjust video camera Osmoscan screen. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 108
8.3.4 - - Osmoscan result screen. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 109
8.3.5 - - Start new measurement. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 110
8.4 - - Performing Deformability curve test. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 112
8.4.1 - - Preparing a blood sample. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 112
8.4.2 - - Insert blood sample deformability curve screen. . . . . . . . . . . . . . . . . . . . . . . . . . 114
8.4.3 - - Adjust video camera deformability screen. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 116
8.4.4 - - Deformability curve result screen. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 117
8.4.5 - - Start new measurement. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 117
8.5 - - Performing Cell stability test. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 118
8.5.1 - - Preparing a blood sample. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 118
8.5.2 - - Insert blood sample stability screen. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 119
8.5.3 - - Adjust video camera stability screen. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 121
8.5.4 - - Stability curve determination results screen. . . . . . . . . . . . . . . . . . . . . . . . . . . . 122
8.5.5 - - Start new measurement. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 122
8.6 - - Performing Cell membrane stability test. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 123
Contents
6MRN-231-EN Version 5.08 (24-04-2023)

8.6.1 - - Preparing a blood sample. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 123
8.6.2 - - Insert blood sample membrane screen. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 124
8.6.3 - - Adjust video camera membrane screen. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 126
8.6.4 - - Cell membrane stability results screen. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 127
8.6.5 - - Deformability results 2 screen. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 128
8.6.6 - - Start new measurement. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 128
8.7 - - Performing RBC Aggregation test. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 129
8.7.1 - - Preparing blood sample for RBC aggregation test. . . . . . . . . . . . . . . . . . . . . . . . 129
8.7.2 - - Insert blood sample RBC aggregation screen. . . . . . . . . . . . . . . . . . . . . . . . . . . 130
8.7.3 - - Adjust temperature RBC aggregation screen. . . . . . . . . . . . . . . . . . . . . . . . . . . . 132
8.7.4 - - RBC aggregation result screen. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 133
8.7.5 - - Start new measurement. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 133
8.8 - - Performing Oxygenscan. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 135
8.8.1 - - Required materials. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 135
8.8.2 - - Pre-test preparations. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 135
8.8.3 - - Cleaning procedure. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 137
8.8.4 - - Preparing a blood sample Oxygenscan. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 137
8.8.5 - - Place sample Oxygenscan test. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 138
8.8.6 - - Oxygenscan . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 140
8.9 - - End of day wash and powering off. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 141
8.10 - - Decontamination procedures Routine. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 143
8.11 - - Waste container disposal. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 144
8.12 - - Powering down. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 144
9 - Reporting. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 146
9.1 - - Deformability Report . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 147
9.2 - - Cell Membrane Stability report page 1 of 3. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 148
9.3 - - Cell Membrane Stability report page 2 of 3. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 149
9.4 - - Cell Membrane Stability report page 3 of 3. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 150
9.5 - - Osmoscan Report. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 151
9.6 - - Oxygen Report (.CSV). . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 152
10 - Troubleshooting. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 154
10.1 - - Error messages. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 154
10.2 - - Error Messages (Oxygenscan). . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 156
10.3 - - Troubleshooting table. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 157
10.4 - - Communication problems. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 158
Contents
MRN-231-EN Version 5.08 (24-04-2023) 7

11 - Maintenance. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 161
11.1 - - Maintenance schedule. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 161
11.2 - - Cleaning. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 162
11.3 - - Oxygen sensor spot protocol. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 162
11.4 - - Replacement sensor-spot. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 163
11.5 - - Visual checks. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 165
11.6 - - Lorrca reagents routine. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 165
11.7 - - Cleaning of the reagent bottles. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 166
11.8 - - Adjusting geometry parameters. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 166
11.9 - - Back up the results file. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 166
11.10 - - Update the software. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 166
11.11 - - Adjustments and replacements. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 167
11.11.1 - - Adjusting projection screen. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 167
11.11.2 - - Replace the main fuses. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 169
11.11.3 - - Waste and rinse pump tube replacements. . . . . . . . . . . . . . . . . . . . . . . . . . . . 170
11.11.3.1 - - Waste and rinse pump tube replacement. . . . . . . . . . . . . . . . . . . . . . . . 170
11.11.3.2 - - Pump connection tubing. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 171
11.11.4 - - Tubing replacements. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 173
11.11.4.1 - - Gradient pump tube replacement. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 173
11.11.4.2 - - Install LORC070901 tubing. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 175
11.11.4.3 - - Install LORC070902 tubing set 1. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 179
11.11.4.4 - - Install LORC070903 tubing set 2. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 182
11.11.4.5 - - Install LORC070904 tubing set 3. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 185
11.11.4.6 - - After replacement of all tubing. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 185
11.11.5 - - Preparation for long time inactivity. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 186
12 - Appendix for Lorrca. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 187
12.1 - - Work instruction. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 188
12.2 - - Appendix - Spare parts. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 192
12.3 - - Appendix - Parts list. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 194
Contents
8MRN-231-EN Version 5.08 (24-04-2023)

13 - Drawings and parts Lorrca. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 196
13.1 - - LORC070020 Tubing diagram. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 197
13.2 - - LORC080020 Connection diagram. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 198
- Log book (Example). . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 199
- Glossary of Terms. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 201
- Index. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 202
Contents
MRN-231-EN Version 5.08 (24-04-2023) 9

Manufacturer: RR Mechatronics Manufacturing B.V.
Phone: [phone]
Fax: [phone]
E-mail: [email protected]
Internet: www.rrmechatronics.com
Postal address:
P.O. Box 225
1620 AE Hoorn
The Netherlands
Office address:
De Corantijn 13
1689 AN Zwaag
The Netherlands
© Copyright RR Mechatronics Manufacturing B.V.
All rights reserved.
Subject to changes without prior notice.
Issued by the Support Department of RR Mechatronics
Contents
10 MRN-231-EN Version 5.08 (24-04-2023)

1
Document history overview
MRN-231-EN
Published date Monday, 24 April 2023
Issue No Date Revised Section(s) Changes Authorised
5.08 April 2023 Lorrca Program
Operational
procedures
Software release 6.05.01:
• Instrument/software
identification added to
Settings screen
• Extra format
Measurement results
H. Schavemaker
5.07 March 2021 Lorrca Program
Operational
procedures
All
• Default settings
Oxygen
• Changed Operational
procedures
• Changed Startup and
EOW procedures
• Editorial changes
H. Schavemaker
5.06 October 2020 Program
Operation • Software release
6.02
• Updated Oxygenscan
procedure
H. Schavemaker
5.05 August 2020 Maintenance • Pump tube
replacement half-year -
>one year
H. Schavemaker
Document history overview
MRN-231-EN Version 5.08 (24-04-2023) 11

5.04 March 2019 Safety
Installation
Operational
instructions
Maintenance
• Mains specification
changed
• Oxygenscan
procedure changed
• Cleaning instructions
changed
• Document History
added as appendix,
only latest changes in
introduction
H. Schavemaker
5.03 January 2019 Installation
Program
Operational
instructions
• Introduction of
Oxygenscan option
H. Schavemaker
5.02 October 2017 Installation
Program • Introduction
Software version 5.xx
• Lorrca 1.01
H. Schavemaker
Document history overview
12 MRN-231-EN Version 5.08 (24-04-2023)

2
About RR Mechatronics
Since being founded in The Netherlands in 1986, we have adhered to the belief in the exponential strength
of technological advancement. RR Mechatronics develops, manufactures and markets worldwide instruments
for scientific research- and medical centres. Microprocessor technology plays a vital role in every aspect. We
have a track record of thinking in terms of solutions, not problems and do not have all the answers, but
persistently strive to find them in close collaboration with research scientists and our other partners. These
partnerships significantly contribute to our high quality work.
RR Mechatronics is ISO 13485:2016 certified by Dekra certification.
The unique collaboration between the scientific world and our research & development department enabled
us to develop the Lorrca Maxsis. This also makes it our responsibility to link and disseminate the knowledge
gained in the worldwide community of researchers and clinicians.
Our people are passionate about the invention and development of highly precise analytic instruments. RR
Mechatronics focuses on medical analytics laboratory instruments. We serve laboratory customers and other
partners all over the world. About 90% of our total production is exported through a worldwide network of
exclusive and qualified distributors.
RR Mechatronics is the world leader in automated true Westergren ESR instruments.
In addition to the haematology related instruments, RR Mechatronics develops very specific environment
related high technology instruments. To complement our line of instruments, we also offer diluents,
cleaning-agents, controls, and specific reagents.
Mechatronics USA is the specialized sales and service organization for RR Mechatronics’ instruments sold in
the United States. We understand that service of daily used instruments must be performed on-site and we
are organized to do so. Mechatronics operates one service number for any service request and is available
24/7.
About RR Mechatronics
MRN-231-EN Version 5.08 (24-04-2023) 13

3
Introduction
3.1 - About this manual
This manual describes all necessary procedures and information required for the correct and successful use
of the Lorrca Maxsis, covering:
•Installation
•Normal operation
•User maintenance
•Troubleshooting
This manual does include details about possible errors, listed in the chapter troubleshooting. Here a
description can be found about known errors of the instrument. If other errors occur, which are not
mentioned in this section, please contact our service department.
RR Mechatronics retains the right to make any changes to the contents without any previous warning. Check
our website for the latest version of this manual .
For information concerning settings, repair and maintenance which is not mentioned in this manual, please
contact RR Mechatronics.
Any remarks or suggestions will be gratefully received. RR Mechatronics accepts no liability for any
mistakes in this User Manual, nor for the consequences of any faulty interpretation of text or drawings.
Read the whole Manual through carefully, especially chapter Safety ,
before using the instrument.
The section LoRRca MaxSis Program is interactive and most of the
software functions are the same as in the program itself.
3.2 - Typographical conventions
Menu selection Items you must select, such as menu options, command buttons, or
items in a list.
Introduction
14 MRN-231-EN Version 5.08 (24-04-2023)

Emphasis Use to emphasise the importance of a point or for variable expressions
such as parameters.
In Software descriptions:
• Tabs like SCAN SETTING
• Text buttons like "Stop", "Save"
• Toggle-buttons like [p-type], [<=X]
• Input parameters like
Current Density, Scan Speed
• Names of the keys on
the keyboard like
[ALT]+[PRINT SCREEN]
Specific warning text is shown as:
Warning: Do not open during operation
3.3 - Used symbols
In this manual, the following graphical symbols are used to draw attention to important information:
This symbol indicates warnings and other danger related information. Ignoring this
information could result in personal injury, damage to the instrument or property or
malfunction of the instrument.
Warning sign to prevent personal injury due to electrical shocks
Warning sign to prevent personal injury due to rotating parts
Introduction
MRN-231-EN Version 5.08 (24-04-2023) 15

Warning sign to prevent personal injury due to biohazard.
Dangerous laser radiation. Avoid direct exposure to laser beam. Laser safety class 3R
Information sign on the supplied materials intended to inform about the “USE BY” date.
This symbol indicates useful tips and other important information, that are not related
to any specific danger.
This symbol indicates a reference to this or other product documentation.
Introduction
16 MRN-231-EN Version 5.08 (24-04-2023)

4
Instrument description
The Lorrca Maxsis consists of the instrument and a control PC (and optional printer).
Main components:
1. Computer and display
2. BOB
3. CUP
4. Projection screen
5. De-ionized water bottle, Waste bottle
6. Pumps
7. Valves
8. Laser
Instrument description
MRN-231-EN Version 5.08 (24-04-2023) 17

9. Osmo HIGH, Osmo LOW, Elon ISO bottles
10. Sample unit
11. Oxygenscan unit (optional)
Instrument description
18 MRN-231-EN Version 5.08 (24-04-2023)

4.1 - Technical specifications
Technical specifications for the Lorrca Maxsis:
Lorrca® instrument models:
Model Model name Catalogue number
Lorrca® MaxSis LORC109003
Mains voltage 100/240V 50-60Hz
Fuse (20 x 5 mm) Slow blow 220V 1.6 Amp
Slow blow 110V 3.15 Amp
Power consumption Standby 100 VA
Maximum (excluding Oxygenscan) 150 VA
Maximum (including Oxygenscan) 200 VA
Heat output Standby 100 Watt
Full operation 150 Watt
Dimensions Width 470 mm
Height with closed lid 400 mm
Height with open lid 630 mm
Depth 300 mm
Total weight
70 Kg
Min. table size Width 1000 mm
Lorrca+Laptop Depth 700 mm
Sound level
< 55 ± 2 dB
Working temperature
15 - 35 °C
Relative humidity
10 - 90%
Specifications of other equipment
Instrument description
MRN-231-EN Version 5.08 (24-04-2023) 19

Camera Objective mount C-mount
Connection USB 3.0
Minimal resolution 780x582 black/white
Dimensions 40x40x40 mm
Laser Wave length 655 nm
Focus distance 170 mm
output power Max 3 mW
Blood sample Tube solution EDTA-Blood
Deformability test +/- 25 ml
Osmoscan +/- 200 ml
Oxygenscan +/- 50 ml
Tech specs Couette assembly
CUP Inner Radius 16.01 ± 0.005 mm
Surface roughness Ra<0.026 µm
Material PMMA
BOB Outer Radius 15.65 ± 0.005 mm
Surface roughness Ra<0.026 µm
Material PMMA
Drive Minimal cup speed 0.03 rps
Maximum cup speed 20 rps
Break time from sudden stop from 106
rpm to 0 rpm
<30 ms
Shear rate
0 - 800 s-1
Warming-up time Bob Maximum 2 minutes
Heater Heating accuracy ± 0.2 °C
Range 25 - 45 °C
Tech specs Gradient assembly
Gradient mixer range 0 - 25 mS/cm
Instrument description
20 MRN-231-EN Version 5.08 (24-04-2023)