Sartorius stedim Sartocube ECO Series User manual

User Manual | Bedienungsanleitung
Sartocube®ECO | Sartocon®ECO |
Sartocon®Slice |
Sartocon®Slice 200 ECO Cassettes
White Silicone | Weißes Silikon
1000062320 Vers. 11|2018

2Contents of this Manual | Inhalt dieser Anleitung
Contents of this Manual |
Inhalt dieser Anleitung
Legal Notices | Impressum ..............................Page | Seite 2
English ..............................................Page 3
Deutsch..............................................Seite 21
If you require further information on crossflow filtration, other than mentioned in
this User Manual, please contact us.
Reproduction, translation and duplication in any form, in part or in full, requires the
written permission of the Sartorius Stedim Biotech GmbH.
For technical information write to:
Sartorius Stedim Biotech GmbH
August-Spindler-Strasse 11
D-37079 Goettingen
Internet: www.sartorius-stedim.com
Edition: November 2018
Ordernumber: 1000062320
Wenn Sie verbindliche Informationen zu bestimmten Eigenschaften Ihrer Crossflow
Cassetten benötigen, welche über die hier beschriebenen hinaus gehen, bitten wir um
Ihre Anfrage.
Nachdruck, Übersetzung und Vervielfältigung in jeglicher Form, auch auszugsweise,
bedürfen der schriftlichen Genehmigung der Sartorius Stedim Biotech GmbH.
Adresse der Technischen Dokumentation:
Sartorius Stedim Biotech GmbH
August-Spindler-Strasse 11
D-37079 Göttingen
Internet: www.sartorius-stedim.com
Germany
Version: November 2018
Bestellnummer: 1000062320

3
Contents
Contents
1. General .............................................................4
1.1 Instructions to this manual.........................................4
1.2 Symbol .........................................................4
1.3 Storage of unused cassettes ........................................4
1.4 Safety instructions ...............................................5
1.5 Components of a Crossflow System..................................7
2. Start up of cassettes..................................................8
2.1 Installing Filter Cassettes in a Stedim Biotech holding device,
Holding Device...................................................8
2.2 Clamp Force for Sartocon®ECO Cassettes.............................9
2.3 Flushing the Filter Cassettes .......................................10
2.4 Integrity Testing ................................................11
2.4.1 Manual Integrity Test ......................................11
2.4.2 Automatic Integrity Test (Pressure Decay) .......................
.......................................................12
2.5 Determining Clean Water Flux for Filter Cassettes (CWF) ...............13
2.6 Filtration.......................................................15
2.7 Cassette Cleaning ...............................................16
2.8 Cassette Disinfection.............................................17
2.9 Cassette Storage ................................................18
2.9.1 Short Term Storage ........................................18
2.9.2 Long Term Storage ........................................18
2.10 Cassette Return Instructions ......................................19
2.11 Disposal........................................................19
3. Appendix ...........................................................20
3.1 Specifications Sartocon®Slice 200 ECO..............................20
3.2 Specifications Sartocon®Slice ECO .................................20
3.3 Specifications Sartocon®ECO......................................20
3.4 Specifications Sartocube®ECO.....................................20

4General
1. General
1.1 Instructions to this manual
t Please be sure to read this user manual prior to use of equipment.
t Please read all safety instructions.
t This user manual is part of the product. Keep it in a safe place
for future reference.
t Replacement manuals can be requested by telephone or it can be downloaded
from our Webpage at: www.sartorius-stedim.com
1.2 Symbol
The following symbols are used in this user manual:
This symbol indicates situations in which improper procedure could lead
to personal injury.
This symbol indicates situations in which improper procedure could lead
to equipment damage.
This symbol indicates that the preservative is not suitable for consumption.
This symbol draws attention to additional information.
1.3 Storage of unused cassettes
The latest date of usage is 3 years from date of manufacture.
The following conditions have to be maintained during storage in order to guarantee
full cassette functionality:
Cassettes must be stored in a closed dry area.
Temperature has to be between 5°C to 40°C and absolutely frost free.
No direct solar radiation.
No direct contact with moisture.
Any mechanical influence or damage must be prevented.
Products with damaged packaging should be discarded.

5
General
1.4 Safety instructions
The preservative is not suitable for consumption.
Do not apply more than the compression stated in the “Specifications”.
Compressing the filter cassettes too tightly will damage them.
The maximum inlet pressure must not be exceeded (see “Specifications“).
Higher pressures will damage the filter cassettes.
Do not allow any type of filter cassette to dry out to avoid loss of filtration
properties.
Make sure that the filter cassettes you plan to use have the required chemical
compatibility before beginning a filtration run in order to avoid damaging the mem-
branes. If you are not sure about the compatibility of a filter cassette with a certain
chemical, please consult your nearest Sartorius Stedim Application specialist.
Avoid abrupt changes in pressure (approximately > 1 bar/sec), such as those that can
occur when the pump is switched on or when the valves settings are changed.
Keep vibration to the lowest level that is technologically possible.
The effective compression on hydraulic holding devices should be kept constant,
especially when the holding device is installed in a large filtration system.
The maximum allowable back pressure on the permeate side must not exceed 0.5 bar
for Hydrosart®UF and 1 bar for PESU UF.
The outer silicone sealing of cassettes should be washed of with DI water,
after submerged storage, before installation into a cassette holding device.
Permeate backpressure is defined as follows:
Permeate pressure is higher than the Retentate pressure
Pfilt > Pret
Pback = Pfilt - Pret
Hydrostatic pressure on the permeate side (height and the inner diameter of the
filtrate tubing) has an impact on backpressure.
Please make sure that the maximum backpressure pressure is never exceeded.
Note
This has to be distinguished from Transmembrane Pressure.
Transmembrane pressure (TMP):
TMP = [(pfeed + pret)/2] - pperm
and from Differential Pressure:
Δp = pfeed - pret
Feed pressure (pfeed) and retentate pressure (pret), both measureable with a gauge.
Permeate pressure (pperm) is the average pressure on the permeate side, measureable
with a gauge.

6General
If the possibility of crystalline particles entering the cassettes cannot be completely
ruled out (for example, due to abrasion in the pumps), a prefilter must be used.
Make sure that crystallization caused by changes in temperature or concentration
cannot occur during operation or storage.
Do not combine cassettes that have a different feed inlet design. Sartocon®cassettes
have alternating oval and round ports.
Preferably a Sartorius Stedim Biotech holder with hydraulic closure, such as a
Sartoflow®10 or Sartoflow®20, should be used with Sartocon®and Sartocube®ECO
cassettes.
If you wish to use older Sartorius Stedim Biotech holders or holding devices supplied
by other manufacturers, you need to apply a different torque pressure when installing
cassettes. To avoid damaging the cassettes, please contact our application specialist.
Make sure that the correct crossflow conditions are maintained during filtration.
Otherwise, the flux rate of the filter cassettes will decrease rapidly during filtration
under static conditions (dead-ended filtration) (refer to “Filtration”.)
The temperature of the retentate must not exceed that given for continuous
operation, which is listed in the “Specifications.” During crossflow filtration, the
temperature will increase over time because the kinetic energy of the pump is
transferred to the liquid undergoing filtration. If the temperature increases beyond
that indicated in the “Specifications,” the pressure resistance of the crossflow
system will decrease.
You should use a prefilter. This will enhance the flux rate and increase the service life
of the filter cassettes.
You should clean the filter cassettes after every filtration run because a layer caused
by fouling builds up on the membranes. Over time, this layer becomes compacted,
making it difficult to remove completely. As a result, this will decrease the flux rate
of your filter cassettes.
All filter cassettes used in one holding device must have identical membranes.
If you do not need to use filter cassettes for a relatively long period, you should store
them in a solution that contains a preservative. This will protect the filter cassettes
from microbial contamination and from drying out.
Do not attempt to operate the filtration system with accessories or spare parts other
than those supplied by the original manufacturer. In the event that the filtration
system is tampered with in any way, we cannot guarantee or take any responsibility
for the performance or safety of the system. This may also endanger the safety of
the operator.
Use only materials recommended by the manufacturer (e.g. gaskets, tubing or hoses.)
Sartocon®Slice 200 Cassette
Sartocon®Slice Cassette
Sartocon®Cassette

7
General
1.5 Components of a Crossflow System
Holding Device
Diagram
Retentate Valve
Pump Permeate
Valve
Holding device
Feed
P2
P1 P3
SARTOFLOW®10 Holder
1–10 Cassettes
Sartocon®Slice 200 (Holder for
max. 2 Slice 200 Cassettes)
Mat. No. #17525---01
Sartocon®Slice
(for 1-5 Slice Cassette Holder
[Sartoflow Alpha])
Mat. No. #17521---002
Sartocon®Slice (for 1-3 Slice
Cassette Holder) Mat. No.
#17521---001 Need for No. #:
1ZLI-V0001 additional long-
spacers to run a Slice 200 cassette
Sartocon®2 plus 1–10 Cassettes
[Order Nr.: 17546*]
SARTOFLOW®20 Holder
2–20 Cassettes

8Start up of cassettes
2. Start up of cassettes
2.1 Installing Filter Cassettes in a Stedim Biotech holding device,
Holding Device
To install the cassettes in a Sartorius Stedim Biotech holding device,
proceed as follows:
1.Remove filter cassette from its packaging.
2.Check and record cassette labelling.
3.Place the filter cassette with the semi-circular cutouts facing downward on the
two guide rods between the two holding plates.
4.Slide the filter cassettes closely together between the two compression plates.
5.Alternately tighten the compression nuts on a manual holder until the required
torque is reached.
6.Apply hydraulic pressure as mentioned in the table “Clamp force for
Sartocon®ECO Cassettes” to the piston by using the supplied manual
or automated hydraulic pump.
7.When using a manual holding devices or a holder with hydraulic handpump
closure, compression has to be checked and eventually re-adjusted every
15 minutes. This has to be repeated until compression is stabilized.
Do not increase the compression pressure beyond the pressure specified.
Preferably a Sartorius Stedim Biotech holder with hydraulic closure, such as a
Sartoflow®10 or Sartoflow®20, should be used with Sartocon®ECO cassettes.
Semi-circular cutouts
SARTOFLOW®Holder
for 2–20 Sartocon®cassettes or up
to 4 Sartocube®cassettes
Guide rods

9
Start up of cassettes
2.2 Clamp Force for Sartocon®ECO Cassettes
If the Sartocon®ECO is installed into a different holder than currently supplied by Sartorius Stedim Biotech, please make sure
to check required compression of the holder with a clamp force measuring device. To avoid cassette damage, please contact our
application specialist.
Preferably a Sartorius Stedim Biotech holder with hydraulic closure, such as a Sartoflow®10 or Sartoflow®20, should be used
with Sartocon®ECO cassettes.
Sartocon®Slice and Slice 200 Holder with manual closure
Required Compression Torque applied on Tie Rods with a Torque Wrench
Operating Conditions Operating Conditions
Hydrosart®UF
Sartocon®ECO Slice 200 3M8144…
Sartocon®Slice ECO 3M5144… 10–14 kN 20 Nm
PESU UF
Sartocon®ECO Slice 200 3M8146…
Sartocon®ECO Slice 200 3M5146… 14–17 kN 25 Nm
New Slice and Slice 200 Holder with trapezoidal thread, PEEK washer and bronze nut – available since April 2014
Membrane Part number Torque applied on tie rods with a torque wrench
Operating conditions
Hydrosart®UF 3M8144… 17 Nm
3M5144… 17 Nm
PESU UF 3M8146… 17 Nm
3M5146… 17 Nm
− After every tenth time of use, the PEEK washers should be replaced.
− The bronze nut or the trapezoidal thread should be replaced as soon as abrasion is visible.
− It is essential that the bronze nut and the trapezoidal thread are kept clean.
Current Sartocon®2 plus holder with manual closure
Required Compression Torque applied on Tie Rods with a Torque Wrench
Operating Conditions Operating Conditions
Hydrosart®UF
Sartocon®ECO Cassettes 3M2144…
Sartocube®ECO 3M2144…-BSW 18–22 kN 60 Nm
PESU UF
Sartocon®ECO Cassette 3M2146…
Sartocube®ECO 3M2146…-BSW 18–25 kN 80 Nm

10 Start up of cassettes
Preferably a Sartorius Stedim Biotech holder with hydraulic closure, such as a Sartoflow®10 or Sartoflow®20,
should be used with Sartocon®ECO cassettes.
Sartoflow®10 | Sartoflow®20 and Sartoflow®Systems with Hydraulic Piston, 12.5 cm2
Required Compression Hydraulic Pressure
Operating Conditions Operating Conditions
Hydrosart®UF
Sartocon®ECO Cassettes 3M2144… 18–22 kN 170 bar (2466 PSI)
Sartocube®ECO 3M2144…-BSW (17 MPa)
PESU UF Sartocon®170 bar (2466 PSI)
Sartocon®ECO Cassettes 3M2146…
Sartocube®ECO 3M2146...-BSW 18–25 kN (17 MPa)
If you wish to use an older Sartorius Stedim Biotech holder or a holding devices supplied by other manufacturers,
you need to apply a different torque or hydraulic pressure. Compression can be checked by using a clamp force
measuring device. To avoid cassette damage, please contact our application specialist.
2.3 Flushing the Filter Cassettes
Flush the filter cassettes as indicated below:
t Before initial use with purified water
t With buffer or physiological saline before filtration
t With buffer or physiological saline before cleaning
t With purified water after cleaning
t With purified water after disinfection
t With purified water before checking the functionality of the filter cassettes
Flush Procedure for Cassettes
The installed cassettes must be flushed with purified water at the recommended
approx. ratio of retentate to permeate listed below.
Cassette Type Ratio of Retentate to Permeate
Sartocon®and Sartocube®ECO Approx. 1 : 1
The cassette must be flushed with 10 l purified water per 0.1 m2filter area.
The permeate and the retentate must be drained.

11
Start up of cassettes
2.4 Integrity Testing
For multiple cassette integrity testing see ”#1 Technical Data Package Integrity
testing Sartocon®Cassettes“.
Ensure the filter cassettes are sufficiently wet before measuring the
air diffusion.
2.4.1 Manual Integrity Test
Proceed as follows:
1.Attach a hose to the feed inlet (V1), and connect the other end of the hose to
a compressed air source.
2.Close the retentate outlet (V2).
3.Adjust the inlet test pressure pinlet to the value specified (see ”Specifications“),
and keep this pressure constant.
4.Close the lower permeate outlet valve V3.
5.At a constant inlet pressure pinlet, wait four minutes until the system
has stabilized.
6.Use a flow meter or an inverted burette filled with water to measure the air
diffusion at the upper permeate outlet.
Fig. 1: System set up for manual integrity testing
V4
Retentate valve
outlet closed
Compressed air Feed
Inlet
V2
P2
P1
P3V3
Permeate
closed
Cassette
V1
Permeate
open

12 Start up of cassettes
2.4.2 Automatic Integrity Test (Pressure Decay)
Proceed as follows:
1.Connect the integrity tester [such as or Sartocheck®4 (16288) or Sartocheck®4
Plus (26288)] to the power supply and pressure source.
2.Close the retentate outlet (V2) and open the permeate outlets (V3, V4). Open V5and
close V1.
3.Enter the required test parameter according to the Sartocheck®instruction manual
and cassette data.
4.Use the following parameter:
stab. time: 4 min.
test time: 2 min.
5.Enter the maximum allowable diffusion value (see Specifications 3.1).
6.Start the Integrity Test.
Fig. 2: System set up for automatic integrity testing. V1–V5: valves; P1–P3: pressure gauge.
If the retentate volume exceeds 9 l for a Sartocheck®4 or 14 l for a
Sartocheck®4 Plus, please contact your Sartorius Stedim application specialist.
Sartocheck®
Retentate outlet
closed
V5
V2
Pump V1V4
V3
Feed inlet
closed
Permeate open
Permeate open
Cassette
P1
P2
P3

13
Start up of cassettes
2.5 Determining Clean Water Flux for Filter Cassettes (CWF)
The water flux test is an easy and non-destructive test that measures the ability
of water to pass through the membrane in a specific system setup. Residuals on the
membrane surface will impact water flux rates. Determining the water flux rate
enables the user to confirm the ability of a cleaning cycle to restore filtration rate of
the system. The water flux should be trended over time and evaluated for potential
membrane and system changes.
It cannot be used to assess the removal of preservative, cleaning or process residue.
Specific analytical techniques are required to quantify residues.
Determine a clean water flux
Proceed as follows:
1.Rinse new installed cassettes to remove preservatives.
2.Post use sanitization cassettes with 1 N Sodium hydroxide and neutralize.
3.Run the clean water flux with purified water as specified in the table below.
4.Measure retentate rate and permeate rate at constant temperature (20°C).
5.Trend the clean water flux and water flux and evaluate data for potential
membrane and system changes. The clean water flux is used as the base line value.
Any changes in water quality, applied pressure, temperature and viscosity,
system design and membrane will have an effect on water flux.
In systems containing more than one Sartocon® ECO, the interpretation can be
simplified by establishing a baseline for a given set of devices in a given system.
Flow path restriction may not allow performing the flux test with the above-
mentioned parameters. In that case a different test pressure should be used to
perform a water flux test. Once the parameters are defined they have to remain
constant during every test to assure accuracy.
System Pressure Examples for Water Flux Determination:
Cassette Design Feed Pressure Retentate Permeate Pressure
valve open Pressure valve open
[psi|bar|kPa] [psi|bar|kPa] [psi|bar|kPa]
Sartocon®Slice
and Slice 200
ECO Cassette 29|2|200 7|0.5|50 0|0|0
Sartocon®ECO
Cassette 29|2|200 7|0.5|50 0|0|0
Multiple Sartocon®29|2|200 10–14|0.7–1|70–100 <7|0.5|50
ECO Cassettes
or Sartocube®ECO

14 Start up of cassettes
Although the flux rate of used filter cassettes will normally not be fully regained,
the flux rate after use and cleaning should approximate the clean water flux.
Trending the water flux helps to evaluate the cleaning status of the system.
Cleaning optimization should be considered:
tIf the permeate flux rate is below 70% of the clean water flux.
tIf the permeate flux rate decreases from run to run.
In case of further questions please contact our Sartorius Stedim application specialist.

15
Start up of cassettes
2.6 Filtration
A critical time during crossflow filtration is start up. The process fluid to be filtered
initially contacts the clean membrane surface. Until the desired steady state process-
ing conditions are attained, a higher than expected permeate flux rate will prevail.
Please consider:
tPartly close permeate valve to limit permeate flow.
Starting a Crossflow filtration process:
1.Prime the permeate and retentate areas with one of the liquids listed below:
tIsotonic physiological saline
tProcessing buffer
To prime the system using one of the liquids listed above, follow the directions given
in the section entitled ”Flushing the Filter Cassettes” section 2.3.
2.Reduce permeate outlet valves setting to allow a small amount of fluid to exit.
3.Generate the desired crossflow rate. The inlet pressure will increase.
4.Open the permeate outlet valve until the desired TMP is attained.
5.Make sure that the desired crossflow rate for the process is maintained.
6.Reset the desired process TMP if required.
The pressure at the feed inlet pinlet must not exceed 58 psi|4 bar.
The maximum allowable backpressure for all Sartocon®ECO cassettes is:
<7 psi|0.5 bar|50 kPa.
During filtration, the crossflow rate should be held constant. Viscosity increase
during filtration will increase the inlet pressure.
7.At the end of filtration, flush the system (see ”Flushing the Filter Cassettes”).
8.After flushing, clean the system (see ”Cleaning the Filter Cassettes”).

16 Start up of cassettes
2.7 Cassette Cleaning
Filter cassettes should be cleaned after every filtration run to prevent a sedi-
ment layer build-up on the membrane and in the retentate channels. Over time,
this layer becomes compacted, making it difficult to remove completely. As a
result, this will decrease the flux rate of your filter cassettes and reduce their
lifespan.
We recommend the following cleaning agents and conditions for the various
membrane materials. This table gives suggested procedures only; requirements may
vary in individual cases:
Cleaning agent Concentration pH Time [min] Temperature [°C]
Sodium hydroxide 1 N 14 60 50
Phosphoric acid 2% by weight 1.3 30 50
Adjust the following pressures:
pinlet = 29 psi|2.0 bar|200 kPa
pret = 0 psi|0 bar|0 kPa
Ultrafilter cassettes:
Recirculate cleaning solution with permeate valves open for 60 minutes.
Repeat as required.
Flush the filter cassettes with purified water as described in “Flushing the Filter
Cassettes”. Determine the flux rate of the cleaned cassette as described in
“Determining clean water flux for filter cassettes”. Compare results with the
previously determined clean water flux (CWF). It is normal to have a greater drop in
clean water flux rate after the first processing and complete cleaning. Good cleaning
practice will result in less water flux deviation.
If the flux rate remains below this level after further cleaning attempts, please
contact your local Sartorius Stedim application specialist.
Generally, the Sartorius product and material limitations, especially regarding pH and
temperature stability of the membranes and cassettes, must be adhered to.
The Sartorius INCREASE®Program offers evaluation of product specific cleaning
procedures.
Detection Method of NaOH cleaning agent clearance:
tNeutral pH effluent in both retentate and permeate.
tConductivity.

17
Start up of cassettes
Example of a basic cleaning protocol for Hydrosart®Ultrafilters:
Step 1:
Flush the filter cassettes with isotonic saline for 5 min. at room temperature.
Conditions:
pin = 36.3 psi|2.5 bar|250 kPa
pret = 0 psi|0 bar|0 kPa
pper = open
Step 2:
Recirculate 1 N NaOH at room temperature for 10 min; then flush the filter cassettes
with the same batch of sodium hydroxide.
Conditions:
pin = 29 psi|2.0 bar|200 kPa
pret = 0 psi|0 bar|0 kPa
pper = open
Step 3:
Flush with purified water until the pH is neutral.
Conditions:
pin = 29 psi|2.0 bar|200 kPa
pret = 14.5 psi|1.0 bar|100 kPa
pper = open
2.8 Cassette Disinfection
Always disinfect clean filter cassettes only. Formaldehyde can denature proteins.
As a result the protein might be irreversibly fixed to the membrane, which will
result in flux decay.
Disinfectant Concentration Time Temperature
Formaldehyde 2–3% by weight 30 min 20–30°C
NaOH 1 N 30 min 40°C
Adjust the following pressures:
pin = 29 psi|2.0 bar|200 kPa
pret = 7.3 psi|0.5 bar|50 kPa
pper = 0 psi|0 bar|0 kPa
Recirculate the disinfectant through the cassette(s).
The disinfectant must flow through both permeate outlets.
Afterwards, flush the filter cassette(s) with purified water as described in
“Flushing the Filter Cassettes”.

18 Start up of cassettes
2.9 Cassette Storage
Sartorius filter cassettes can be stored after use. To avoid microbial
contamination preservatives have to be used.
All membranes will lose their filtration performance if allowed to dry out or
freeze. Always store filter cassettes in moist condition.
2.9.1 Short Term Storage
We recommend the following preservatives for short term storage
(not exceeding 4 weeks):
Preservative Concentration
Formaldehyde 2–3% by weight
Ethanol 20%–40% by weight *
Do not use denatured alcohol
NaOH 0.1 N
* Hydrosart®can be stored in Ethanol 20–40% by weight.
For short term storage proceed as follows:
1.Clean the filter cassettes (see “Cleaning the Filter Cassettes”).
2.Recirculate the preservative for 5 minutes through the system at the same flow
rate used to flush the filter cassettes. The preservative must flow through both
permeate outlets.
3.Close feed inlet, retentate outlet and both permeate outlets.
4.Do not allow the cassettes to dry out or freeze.
2.9.2 Long Term Storage
We recommend the following preservatives for long term storage:
Preservative Concentration
Formaldehyde 2–3% by weight
Ethanol 20%–40%by weight*
Do not use denatured alcohol
NaOH 0.1 N PESU and Hydrosart®up to 12 months
* Hydrosart®can be stored in Ethanol 20–40% by weight.

19
Start up of cassettes
For long term storage proceed as follows:
1. Clean the filter cassettes (see “Cleaning the Filter Cassettes”).
2. Recirculate the preservative for 5 minutes through the system at the same flow
rate used to flush the filter cassettes. The preservative must flow through both
permeate outlets.
3. Remove the filter cassette from the holding device.
4. Store the filter cassettes in a closed container or bag as follows:
t Plastic bag:
Seal the filter cassette together with 50 ml of preservative into the bag.
t Container:
Pour preservative over the filter cassette until it is completely immersed;
then close the container.
The storage solution has to be washed of the outer surface with purified water
before installation into a holding device.
2.10 Cassette Return Instructions
If cassettes are sent back to Sartorius Stedim for evaluation, please proceed
as follows:
1. Inform your local Sartorius Stedim Application Support.
2. Clean the cassettes completely (see “Cleaning the Filter Cassettes“).
3. Disinfect the cassettes (see current product return form).
4. Include the following information:
a. Specify product or media filtered
b. Describe failure observed
c. Reveal operating parameters such as
– operating pressure
– time in use
– process cycles
– cleaning conditions
– process temperature
d. Number of cleaning cycles
e. Description of disinfectant.
2.11 Disposal
If non-hazardous residual impurities are present, the EWC waste code 150203
(European Waste Catalogue) for absorbents and filter materials, wiping cloths and
protective clothing can be applied. If the filters are contaminated with hazardous
materials, the EWC waste code 150202* for absorbents and filter materials
contaminated with hazardous materials should be used. If you are uncertain, contact
your waste disposal authority.

20 Appendix
3. Appendix
3.1 Specifications Sartocon®Slice 200 ECO
Membrane Nominal molecular Order numbers Effective pH Thermal Maximum Maximum temp. [°C] Air diffusion Integral Preservation1)
material weight cutoff filtration area stability steriliz- pfeed [bar] for continuous value ml/min Frame
[NMWCO] [m2] ation at 20°C operation at 1 bar
Polyether- 10 kD 3M81463902E--SW 0.02 1-14 No 4 50 ≤1.0 PP Ethanol
sulfon 30 kD 3M81465902E--SW 0.02 1-14 No 4 50 ≤1.0 PP Ethanol
(PESU) 100 kD 3M81466802E--SW 0.02 1-14 No 4 50 ≤1.0 PP Glycerin
300 kD 3M81467902E--SW 0.02 1-14 No 4 50 ≤1.0 PP Glycerin
Hydrosart®10 kD 3M81443902E--SW 0.02 2-14 No 4 50 ≤1.0 PP Ethanol
30 kD 3M81445902E--SW 0.02 2-14 No 4 50 ≤1.0 PP Ethanol
100 kD 3M81446802E--SW 0.02 2-14 No 4 50 ≤1.0 PP Ethanol
300 kD 3M81447902E--SW 0.02 2-14 No 4 50 ≤1.0 PP Ethanol
3.2 Specifications Sartocon®Slice ECO
Membrane Nominal molecular Order numbers Effective pH Thermal Maximum Maximum temp. [°C] Air diffusion Integral Preservation1)
material weight cutoff filtration area stability steriliz- pfeed [bar] for continuous value ml/min Frame
[NMWCO] [m2] ation at 20°C operation at 1 bar
Polyether- 10 kD 3M51463901E--SW 0.14 1-14 No 4 50 ≤5.0 PP Ethanol
sulfon 30 kD 3M51465901E--SW 0.14 1-14 No 4 50 ≤5.0 PP Ethanol
(PESU) 100 kD 3M51466801E--SW 0.14 1-14 No 4 50 ≤5.0 PP Glycerin
300 kD 3M51467901E--SW 0.14 1-14 No 4 50 ≤5.0 PP Glycerin
Hydrosart®10 kD 3M51443901E--SW 0.14 2-14 No 4 50 ≤5.0 PP Ethanol
30 kD 3M51445901E--SW 0.14 2-14 No 4 50 ≤5.0 PP Ethanol
100 kD 3M51446801E--SW 0.14 2-14 No 4 50 ≤5.0 PP Ethanol
300 kD 3M51447901E--SW 0.14 2-14 No 4 50 ≤5.0 PP Ethanol
3.3 Specifications Sartocon®ECO
Membrane Nominal molecular Order numbers Effective pH Thermal Maximum Maximum temp. [°C] Air diffusion Integral Preservation1)
material weight cutoff filtration area stability steriliz- pfeed [bar] for continuous value ml/min Frame
[NMWCO] [m2] ation at 20°C operation at 1 bar
Polyether- 10 kD 3M21463907E--SW 0.7 1-14 No 4 50 ≤50.0 PP Ethanol
sulfon 30 kD 3M21465907E--SW 0.7 1-14 No 4 50 ≤50.0 PP Ethanol
(PESU) 100 kD 3M21466807E--SW 0.7 1-14 No 4 50 ≤50.0 PP Glycerin
300 kD 3M21467907E--SW 0.7 1-14 No 4 50 ≤50.0 PP Glycerin
Hydrosart®10 kD 3M21443907E--SW 0.7 2-14 No 4 50 ≤15.0 PP Ethanol
30 kD 3M21445907E--SW 0.7 2-14 No 4 50 ≤15.0 PP Ethanol
100 kD 3M21446807E--SW 0.7 2-14 No 4 50 ≤15.0 PP Ethanol
300 kD 3M21447907E--SW 0.7 2-14 No 4 50 ≤15.0 PP Ethanol
3.4 Specifications Sartocube®ECO
Membrane Nominal molecular Order numbers Effective pH Thermal Maximum Maximum temp. [°C] Air diffusion Integral Preservation1)
material weight cutoff filtration area stability steriliz- pfeed [bar] for continuous value ml/min Frame
[NMWCO] [m2] ation at 20°C operation at 1 bar
Polyether- 10 kD 3M21463935E-BSW 3.5 1-14 No 4 50 ≤50.0 PP Ethanol
sulfon 30 kD 3M21465935E-BSW 3.5 1-14 No 4 50 ≤50.0 PP Ethanol
(PESU) 100 kD 3M21466835E-BSW 3.5 1-14 No 4 50 ≤50.0 PP Glycerin
300 kD 3M21467935E-BSW 3.5 1-14 No 4 50 ≤50.0 PP Glycerin
Hydrosart®10 kD 3M21443935E-BSW 3.5 2-14 No 4 50 ≤50.0 PP Ethanol
30 kD 3M21445935E-BSW 3.5 2-14 No 4 50 ≤50.0 PP Ethanol
100 kD 3M21446835E-BSW* 3.5 2-14 No 4 50 ≤50.0 PP Ethanol
300 kD 3M21447935E-BSW* 3.5 2-14 No 4 50 ≤50.0 PP Ethanol
1) The preservation liquid is either Ethanol 20% by weight or Glycerin.
* Product available on request only.