Sartorius stedim Vivaspin Turbo 15 Instruction manual

85032-541-63
Technical data and operating instructions
Vivaspin®Turbo 15
For in vitro use only

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Vivaspin®Turbo 15 – Introduction
Storage conditions|shelf life
Vivaspin®Turbo 15 ultrafiltration
spin columns should be stored at room
temperature. The devices should be
used before the expiry date printed on
the box.
Introduction
Vivaspin®Turbo 15 centrifugal concentrators
offer the optimal solution to any concentra-
tion or buffer exchange application with
their broad range of MWCOs.
Highest flow rates are achieved due to their
double verticle membranes which minimize
protein polarization and subsequent fouling
of the membrane. Additionally, their sleek
internal profile ensures maximum process
speeds right down to the last 150 μl.The UV
joining technology allows for a smooth joint
transition between membrane and plastic
housing, allowing complete sample recovery
from the unique pipette friendly deadstop
pocket. The dead stop pocket (patent pend-
ing) is an impermeable concentrate pocket
integrally moulded below the membrane sur-
face, thereby eliminating the risk of filtration
to dryness.
The Vivaspin®Turbo 15 devices are the opti-
mal solution for protein concentration from
dilute samples e.g. after chromatography,
cell culture supernatants or fermentation
broths.
Operation
Vivaspin®Turbo 15 concentrators can be
used in swing bucket or fixed angle rotors
accepting standard conical bottom tubes. In
a single spin, solutions can be concentrated
up to 150 +. Samples are typically concen-
trated in 10 to 30 minutes with macromole-
cular recoveries in excess of 95%.
Equipment Required
For use with centrifuge
1. Centrifuge with swing bucket or fixed
angle rotor (minimum 25°).
2. Pasteur or fixed volume pipettes for
sample delivery and removal.
Device Carrier Required
Vivaspin®Turbo 15 50 ml/30 mm d

Equipment Required
Equipment required Vivaspin®Turbo 15
Centrifuge
Rotor type Swing bucket Fixed angle
Minimum rotor angle – 25°
Rotor cavity Conical bottom tubes
Rotor compatibility
Please note: Vivaspin®Turbo 15 (30 mm +
118 mm) is designed to fit into rotors that
can accommodate Falcon 50 ml conical
bottom tubes, e.g. Beckman Allegra 25R
with TS-5.1-500 swing-out rotor with BUC
5 buckets and 368327 adaptors; Beckman
TA-10.250 25° fixed angle rotor with
356966 adaptors; Heraeus Multifuge 3 S-R
with (Heraeus/Sorvall) 75006445 swing out
rotor with 75006441 buckets and adaptors
for Falcon 50 ml conical bottom tubes.
These devices are not designed to fit into
rotors that only accept round bottom
29 mm +105 mm tubes, e.g. Sorvall SS34
or Beckman JA 20. If your rotor accepts
only 29 mm +105 mm round bottom tubes,
please use the Vivaspin®15, which can be
used in either round bottom or conical
centrifuge tubes.
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Desalting|Buffer Exchange
1. Concentrate sample to desired level.
2. Empty filtrate container.
3. Refill concentrator with an appropriate
solvent.
4. Concentrate the sample again and repeat
the process until the concentration of
contaminating microsolute is sufficiently
reduced. Typically, 3 wash cycles will remove
99% of initial salt content.
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Operation
1. Select the most appropriate membrane
cut-off for your sample. For maximum
recovery select a MWCO at least 50% smaller
than the molecular size of the species of
interest.
2. Fill concentrator with up to maximum
volumes shown in table 1. (Ensure screw
closure is fully seated)
3. Insert assembled concentrator into
centrifuge (when fixed angle rotors are
used, angle concentrator so that the printed
window faces upwards|outwards).
4. Centrifuge at speeds recommended
in table 2, taking care not to exceed the
maximum g force indicated by membrane
type and MWCO
5. Once the desired concentration is
achieved, (see table 3a and 3b for guide
to concentration times), remove assembly
and recover sample from the bottom of
the concentrate pocket with a pipette.

Technical Specifications
Table 1: Technical Specifications
Vivaspin®Turbo 15
Dimensions
Total Length 77 mm
30 mm OD flange) 118 in tube
Width 27 mm
Active membrane area 7.2 cm2
Hold up volume of membrane <10 μl
Dead stop volume* in swing out 100 μl
Dead stop volume
in fixed angle (25°) 60 μl
Concentrator Capacity
Swing bucket rotor 15 ml
Fixed angle rotor 11 ml
Materials of construction
Concentrator insert Styrene butadiene block co-polymer
Concentrator cap and tube Polypropylene
Membrane Polyethersulfone
Table 2: Recommended Spin Speed (xg)
Vivaspin®Turbo 15 Centrifuge
Rotor Swing Bucket Fixed Angle
Membrane max max
3–50,000 MWCO PES 4,000 4,000
>100–300,000 MWCO PES 4,000 4,000
* Dead stop volume as designed in moulding tool. This volume may vary depending on
sample, sample concentration, operation temperature and centrifuge rotor.
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6|
Usage Tips
1. Flow Rate
Filtration rate is affected by several parame-
ters, including MWCO, porosity, sample con-
centration, viscosity, centrifugal force and
temperature. Expect significantly longer spin
times for starting solutions with over 5%
solids. When operating at 4°C. flow rates are
approximately 1.5 times slower than at 25°C.
Viscous solutions such as 50% glycerine will
take up to 5 times longer to concentrate
than samples in a predominantly buffer
solution.
2. Pre-rinsing
Membranes fitted to Vivaspin®concentrators
contain trace amounts of Glycerine and
Sodium azide. Should these interfere with
analysis they can be removed by rinsing fill
volume of buffer solution or deionised water
through the concentrator. Decant filtrate
and concentrate before processing sample
solution. If you do not want to use the
pre-rinsed device immediately, store it in the
refrigerator with buffer or water covering
the membrane surface. Please do not allow
the membrane to dry out.
3. Sterilisation of Polyethersulfone
Membranes
Polyethersulfone membranes should not
be autoclaved as high temperatures will
substantially increase membrane MWCO.
To sterilise, use a 70% ethanol solution or
sterilising gas mixture.
4. Chemical Compatibility
Vivaspin®concentrators are designed for use
with biological fluids and aqueous solutions.
For chemical compatibility details, refer to
table 4.

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Performance Characteristics
Table 3: Performance Characteristics Vivaspin®Turbo™ 15
Time to concentrate up to 20x [min.] at 20°C
and solute recovery %
Mode Centrifuge Centrifuge
Rotor Swing 25° Fixed
bucket angle
Start volume 15 ml 11 ml
Min. Rec. Min. Rec.
Cytochrome c 0.25 mg/ml (12,400 MW)
3,000 MWCO PES
a-Chymotrypsin 1.0 mg/ml (25,000 MW)
10,000 MWCO PES 10 97% 10 97%
BSA 1.0 mg/ml (66,000 MW)
5,000 MWCO PES
10,000 MWCO PES 10 95% 10 95%
30,000 MWCO PES
IgG 0.25 mg/ml (160,000 MW)
30,000 MWCO PES
50,000 MWCO PES
100,000 MWCO PES
Latex beads 0.004% in DMEM +10% FCS (0.055 μm)
300,000 MWCO PES
Latex beads 0.004% in DMEM +10% FCS (0.24 μm)
1,000,000 MWCO PES
Yeast 1.0 mg/ml (S. Cerevisiae)
0.2 μm PES

Chemical Compatibility
Table 4: Chemical Compatibility (2hr contact time)
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Solutions PES
Compatible pH range pH 1–14
Acetic Acid (25.0%) OK
Acetone (10.0%) OK
Acetonitrile (10.0%) OK
Ammonium Hydroxide (5.0%) ?
Ammonium Sulphate (saturated) OK
Benzene (100%) NO
n-Butanol (70%) OK
Chloroform (1.0%) NO
Dimethyl Formamide (10.0%) ?
Dimethyl Sulfoxide (5.0%) OK
Ethanol (70.0%) OK
Ethyl Acetate (100%) NO
Formaldehyde (30%) OK
Formic Acid (5.0%) OK
Glycerine (70%) OK
Guanidine HCI (6 M) OK
Hydrocarbons, aromatic NO
Hydrocarbons, chlorinated NO
Hydrochloric Acid (1 M) OK
Imidazole (500 mM) OK
Isopropanol (70%) OK
Solutions PES
Compatible pH range pH 1–14
Lactic Acid (5.0%) OK
Mercaptoethanol (10 mM) OK
Methanol (60%) OK
Nitric Acid (10.0%) OK
Phenol (1.0%) ?
Phosphate Buffer (1.0 M) OK
Polyethylene Glycol (10%) OK
Pyridine (100%) ?
Sodium Carbonate (20%) ?
Sodium Deoxycholate (5.0%) OK
Sodium Dodecylsulfate (0.1 M) OK
Sodium Hydroxide (2M) OK
Sodium Hypochlorite (200 ppm) ?
Sodium Nitrate (1.0%) OK
Sulfamic Acid (5.0%) OK
Tetrahydrofuran (5.0%) NO
Toluene (1.0%) NO
Trifluoroacetic Acid (10%) OK
Tween 20 (0.1%) OK
Triton X-100 (0.1%) OK
Urea (8 M) OK
OK = Acceptable ? = Questionable NO = Not recommended

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Ordering Information
Cut off Pack size Prod. no.
3,000 MWCO 12 VS15T91
3,000 MWCO 48 VS15T92
5,000 MWCO 12 VS15T11
5,000 MWCO 48 VS15T12
10,000 MWCO 12 VS15T01
10,000 MWCO 48 VS15T02
30,000 MWCO 12 VS15T21
30,000 MWCO 48 VS15T22
50,000 MWCO 12 VS15T31
50,000 MWCO 48 VS15T32
100,000 MWCO 12 VS15T41
100,000 MWCO 48 VS15T42
300,000 MWCO 12 VS15T51
300,000 MWCO 48 VS15T52
1000,000 MWCO 12 VS15T61
1000,000 MWCO 48 VS15T62

10 |
Other Products
Product Sample volume Mode Membranes available
Vivaspin®500 100 μl–600 μl Centrifugal Polyethersulfone
Vivaspin®2 0.4 ml–2 ml Centrifugal Polyethersulfone,
Cellulose Triacetate,
Hydrosart®
Centrisart 0.5 ml–2.5 ml Centrifugal Polyethersulfone,
Cellulose Triacetate
Vivaspin®4 1 ml–4 ml Centrifugal Polyethersulfone
Vivaspin®6 2 ml–6 ml Centrifugal Polyethersulfone
Vivaspin®15 2 ml–15 ml Centrifugal Polyethersulfone
Vivaspin®15R 2 ml–15 ml Centrifugal Hydrosart®
Vivaspin®20 5 ml–20 ml Centrifugal Polyethersulfone
Gas pressure
Vivacell 70 10 ml–70 ml Centrifugal Polyethersulfone
Gas pressure
Vivacell 100 20 ml–100 ml Centrifugal Polyethersulfone
Gas pressure
Vivacell 250 50 ml–250 ml Gas pressure Polyethersulfone
Vivaflow 50 100 ml–>5 l Tangential flow Polyethersulfone,
Regenerated Cellulose
Vivaflow 200 500 ml–>5 l Tangential flow Polyethersulfone,
Regenerated Cellulose,
Hydrosart®
Vivapore 5 1 ml–5 ml Solvent absorption Polyethersulfone
Vivapore 10/20 2 ml–10 ml/20 ml Solvent absorption Polyethersulfone

Sartorius Stedim Biotech GmbH
August-Spindler-Strasse 11
37079 Goettingen, Germany
Phone [phone]
Fax [phone]
www.sartorius-stedim.com
Copyright by Sartorius Stedim
Biotech GmbH, Goettingen,
Germany. All rights reserved.
No part of this publication may
be reprinted or translated in any
form or by any means without
the prior written permission of
Sartorius Stedim Biotech GmbH.
The status of the information,
specifications and illustrations
in this manual is indicated by
the date given below. Sartorius
Stedim Biotech GmbH reserves
the right to make changes
to the technology, features,
specifications and design of
the equipment without notice.
Status:
December 2011,
Sartorius Stedim Biotech GmbH,
Goettingen, Germany
Printed in Germany on paper that has
been bleached without any use of chlorine
Vivaspin®Turbo 15 · W
Publication No.: SLU6126-e12012
Ver. 01 | 2012