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to change the filter daily (after 50 - 250 pipetting
cycles) and always in case of over-aspiration. (See
Chapter 2 for ordering information.)
The Safe-Cone Filters can be removed with the
unique filter ejector without touching the filters
by hand. Remove the operating button cap and
eject the filter by pressing the operating button
down. Make sure that the filter is disposed of into
a suitable waste container. Clean the tip cone, if
needed, and put the new filter in place.
9. Pipetting techniques
Two basic pipetting techniques, forward and
reverse pipetting, are associated with mLINE
pipettes. Forward pipetting is the most common
used pipetting technique. The technique employs
the blow-out function ensuring complete delivery
of the liquid. Reverse pipetting is recommended
for highly viscous, biological or foaming liquids,
or very small volumes of liquid. A selected
volume plus an excess is aspirated into the tip.
The delivery is done without blow-out, and, thus,
the excess volume remains in the tip. The reverse
technique also facilitates the repeated delivery of
the same volume. If reverse pipetting technique is
used the pipette might need recalibration.
9.1. Forward pipetting
1. Fit the tip onto the pipette tip cone.
2. Press the operating button to the first stop.
3. Place the tip just under the surface of the
liquid (2-3 mm) and smoothly release the
operating button up to the starting position.
Wait one second. Carefully withdraw the tip
from the liquid, touching against the edge
of the container to remove excess from the
outside of the tip.
4. Dispense the liquid by pressing the operating
button to the first stop. After a short delay
press the operating button to the second
stop. This action will empty the tip.
9.2. Reverse pipetting
1. Fit the tip onto the pipette tip cone.
2. Press the operating button all the way to the
second stop.
3. Place the tip just under the surface of the
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