SCIEX C100HT Manual

C100HT Biologics Analyzer System
Operator Guide
June 2018RUO-IDV-05-3828-A

This document is provided to customers who have purchased SCIEX equipment to use in the operation of such SCIEX
equipment. This document is copyright protected and any reproduction of this document or any part of this document is
strictly prohibited, except as SCIEX may authorize in writing.
Software that may be described in this document is furnished under a license agreement. It is against the law to copy, modify,
or distribute the software on any medium, except as specifically allowed in the license agreement. Furthermore, the license
agreement may prohibit the software from being disassembled, reverse engineered, or decompiled for any purpose. Warranties
are as stated therein.
Portions of this document may make reference to other manufacturers and/or their products, which may contain parts whose
names are registered as trademarks and/or function as trademarks of their respective owners. Any such use is intended only
to designate those manufacturers' products as supplied by SCIEX for incorporation into its equipment and does not imply
any right and/or license to use or permit others to use such manufacturers' and/or their product names as trademarks.
SCIEX warranties are limited to those express warranties provided at the time of sale or license of its products and are SCIEX’s
sole and exclusive representations, warranties, and obligations. SCIEX makes no other warranty of any kind whatsoever,
expressed or implied, including without limitation, warranties of merchantability or fitness for a particular purpose, whether
arising from a statute or otherwise in law or from a course of dealing or usage of trade, all of which are expressly disclaimed,
and assumes no responsibility or contingent liability, including indirect or consequential damages, for any use by the purchaser
or for any adverse circumstances arising therefrom.
For research use only. Not for use in diagnostic procedures.
AB Sciex is doing business as SCIEX.
The trademarks mentioned herein are the property of AB Sciex Pte. Ltd. or their respective owners.
AB SCIEX™is being used under license.
©2018 AB Sciex
AB Sciex LLC
500 Old Connecticut Path
Framingham, Massachusetts 01701
USA
Operator GuideC100HT Biologics Analyzer System
RUO-IDV-05-3828-A2 / 122

1 Operational Precautions and Limitations...............................................................................................6
General Safety Information................................................................................................................................................6
Regulatory Compliance......................................................................................................................................................6
Australia and New Zealand..........................................................................................................................................6
Canada........................................................................................................................................................................7
Europe.........................................................................................................................................................................7
United States...............................................................................................................................................................7
International................................................................................................................................................................8
Electrical Precautions.........................................................................................................................................................8
AC Mains Supply..........................................................................................................................................................8
Protective Earth Conductor..........................................................................................................................................9
Chemical Precautions.........................................................................................................................................................9
Environmental Precautions..............................................................................................................................................10
Electromagnetic Environment....................................................................................................................................10
Decommissioning and Disposal..................................................................................................................................11
Equipment Use and Modification.....................................................................................................................................11
Qualified Personnel..........................................................................................................................................................12
Contact Us.......................................................................................................................................................................12
Technical Support............................................................................................................................................................12
Documentation Symbols and Conventions.......................................................................................................................13
2 Introduction............................................................................................................................................14
Description.......................................................................................................................................................................14
Hardware Overview.........................................................................................................................................................15
Cartridge..........................................................................................................................................................................17
Theory of Operation.........................................................................................................................................................18
3 Prepare the Samples..............................................................................................................................19
Safety...............................................................................................................................................................................19
Overview..........................................................................................................................................................................19
Intended Use..............................................................................................................................................................19
Equipment and Materials Required..................................................................................................................................20
Customer-Supplied Equipment and Supplies..............................................................................................................20
Customer-Supplied Reagents.....................................................................................................................................20
Prepare the Reagents, Stock Solutions, and Master Mixes..............................................................................................21
Reconstitute the Bracketing Standard (BST-10).........................................................................................................21
Reconstitute the Internal Standard (IST)....................................................................................................................21
Reconstitute the Fluorophore Label...........................................................................................................................21
Prepare the Denaturation Solution.............................................................................................................................21
Prepare the Digestion Solution..................................................................................................................................22
C100HT Biologics Analyzer SystemOperator Guide
3 / 122RUO-IDV-05-3828-A
Contents

Prepare the Labeling Solution....................................................................................................................................23
Denature the Proteins......................................................................................................................................................24
Release and Label the N-Glycans.....................................................................................................................................25
Tips for Best Results...................................................................................................................................................25
Release the N-Glycans...............................................................................................................................................25
Label the Released N-Glycans....................................................................................................................................26
Clean Up the Samples................................................................................................................................................26
Waste Disposal................................................................................................................................................................28
4 Normalize the Cartridge........................................................................................................................29
The Normalization Tab Toolbar........................................................................................................................................33
View Normalization Data in Data View Mode.................................................................................................................33
5 Separate the Glycans.............................................................................................................................35
Prepare the Buffer Plate...................................................................................................................................................35
Load the Buffer Plate.......................................................................................................................................................37
Install the Cartridge in the System...................................................................................................................................38
Condition the Cartridge...................................................................................................................................................40
Perform a Run..................................................................................................................................................................41
Start a Run as a Basic or Advanced User...................................................................................................................42
Analyze the Data and Review the Results..................................................................................................................49
Store the Cartridge...........................................................................................................................................................52
Short-Term Storage....................................................................................................................................................52
Long-Term Storage.....................................................................................................................................................53
6 Review the Results with the DataReviewer Software........................................................................54
About the DataReviewer Software Matching...................................................................................................................54
Create a Qualification Profile...........................................................................................................................................55
Assign a Qualification Profile to a Plate...........................................................................................................................55
View the Qualification Profile Assigned to a Well............................................................................................................56
View the Data Trace for a Well........................................................................................................................................57
Zoom and Scroll the Electropherogram......................................................................................................................59
Manually Integrate the Data......................................................................................................................................60
View Reports....................................................................................................................................................................64
View the Data Report.................................................................................................................................................64
View the Plate Report................................................................................................................................................67
Analyze the Data Manually..............................................................................................................................................70
Analyze the Data from the C100HT Software............................................................................................................70
Open a Plate..............................................................................................................................................................71
7 Routine Maintenance.............................................................................................................................73
Clean the Surfaces...........................................................................................................................................................73
Replace the Fuse..............................................................................................................................................................73
Test the Detector..............................................................................................................................................................74
Test the Filter...................................................................................................................................................................74
Fill the Capillaries and Cartridge......................................................................................................................................75
Test System Movement....................................................................................................................................................76
Replace the Purge Filter...................................................................................................................................................76
Manage Users..................................................................................................................................................................77
Operator GuideC100HT Biologics Analyzer System
RUO-IDV-05-3828-A4 / 122
Contents

Default Users.............................................................................................................................................................78
Add a New User.........................................................................................................................................................79
Modify an Existing User.............................................................................................................................................80
8 Prepare the System to Be Moved.........................................................................................................81
9 Troubleshooting.....................................................................................................................................83
Troubleshooting Tables....................................................................................................................................................83
Run a Test Sample...........................................................................................................................................................88
Export Log Files for Troubleshooting................................................................................................................................89
A System Specifications............................................................................................................................91
Controller and Monitor Specifications..............................................................................................................................91
B Reagents and Accessories.....................................................................................................................93
Order Parts.......................................................................................................................................................................93
Reagents and Accessories................................................................................................................................................93
C100HT Glycan Labeling and Analysis Kit..................................................................................................................93
C Hazardous Substance Information.......................................................................................................95
D Start a Run as a Routine User...............................................................................................................97
E Additional Functions in the Data Trace Tab.......................................................................................103
New Trace Properties Dialog..........................................................................................................................................103
Open Data File Dialog....................................................................................................................................................104
Axis Properties Dialog....................................................................................................................................................105
Trace Annotation Properties Dialog...............................................................................................................................106
Appearance Properties Dialog........................................................................................................................................107
The Operations Submenu...............................................................................................................................................108
The Utilities Submenu....................................................................................................................................................109
The Graphical Programming Submenu...........................................................................................................................109
Data Graph Properties Dialog........................................................................................................................................112
F Glossary of Symbols.............................................................................................................................114
G Glossary of Warnings..........................................................................................................................119
Index........................................................................................................................................................120
C100HT Biologics Analyzer SystemOperator Guide
5 / 122RUO-IDV-05-3828-A
Contents

Note: Before operating the system, carefully read all of the sections of this guide.
This section contains general safety-related information and provides regulatory compliance information. It also
describes potential hazards and associated warnings for the system and the precautions that should be taken to
minimize the hazards.
In addition to this section, refer to Glossary of Symbols for information about the symbols and conventions used
in the laboratory environment, on the system, and in this documentation. Refer to the Site Planning Guide
for site requirements.
General Safety Information
To prevent personal injury or system damage, read, understand, and obey all of the safety precautions and warnings
in this document, the manufacturer chemical safety data sheet (SDS), and product label information. These labels
are shown with internationally recognized symbols. Failure to heed these warnings could result in serious injury.
This safety information is intended to supplement federal, state, provincial, and local environmental health and
safety (EHS) regulations. It does not cover every safety procedure that should be practised. Ultimately, the user
and the organization are responsible for compliance with federal, state, provincial, and local EHS regulations and
for maintaining a safe laboratory environment.
Refer to the appropriate laboratory reference material and standard operating procedures.
Regulatory Compliance
This system complies with the regulations and standards listed in this section. Refer to the Declaration of Conformity
included with the system and the individual system components for dated references. Applicable labels have been
affixed to the system.
Australia and New Zealand
•Electromagnetic Compatibility (EMC): Radio Communications Act 1992 as implemented in these
standards:
•Electromagnetic Interference—AS/NZS CISPR 11/ EN 55011/ CISPR 11 (Class A). Refer to Electromagnetic
Interference.
•Safety: AS/NZ 61010-1 and IEC 61010-2-081
Operator GuideC100HT Biologics Analyzer System
RUO-IDV-05-3828-A6 / 122
1
Operational Precautions and
Limitations

Canada
•Electromagnetic Interference (EMI): CAN/CSA CISPR11. This ISM device complies with Canadian
ICES-001. Refer to Electromagnetic Interference.
•Safety:
•CAN/CSA C22.2 No. 61010-1
•CAN/CSA 222.2 No. 60101-2-081
Europe
•Electromagnetic Compatibility (EMC): Electromagnetic Compatibility directive 2014/30/EU as
implemented in these standards:
•EN 61326-1
•EN 55011 (Class A)
Refer to Electromagnetic Compatibility.
•Safety: Low Voltage Directives 2014/35/EU and Machinery Directive 2006/42/EC as implemented in these
standards:
•EN 61010-1
•EN 61010-2-081
•Waste Electrical and Electronic Equipment (WEEE): Waste Electrical and Electronic Equipment 2012/
96/EEC, as implemented in EN 40519. Refer to Waste Electrical and Electronic Equipment.
•Packaging and Packaging Waste (PPW): Packaging and Packaging Waste Directive 94/62/EC
•RoHS Restriction of Hazardous Substances: RoHS Directive 2011/65/EU
United States
•Radio Emissions Interference Regulations: 47 CFR 15, as implemented in FCC Part 15 (Class A)
•Safety: Occupational Safety and Health Regulations, 29 CFR 1910, as implemented in these standards:
•UL 61010-1
•UL 61010-2-081
C100HT Biologics Analyzer SystemOperator Guide
7 / 122RUO-IDV-05-3828-A
Operational Precautions and Limitations

International
•Electromagnetic Compatibility (EMC):
•IEC 61326-1
•IEC CISPR 11 (Class A)
Refer to Electromagnetic Compatibility.
•Safety:
•IEC 61010-1
•IEC 61010-2-081
Electrical Precautions
WARNING! Electrical Shock Hazard. Do not remove the covers. Removing the
covers might cause injury or malfunctioning of the system. The covers need
not be removed for routine maintenance, inspection, or adjustment. Contact
a SCIEX Field Service Employee (FSE) for repairs that require the covers to be
removed.
•Follow required electrical safe work practices.
•Use cable management practices to control electrical cables. This will reduce the chance of a tripping hazard.
For information about system electrical specifications, refer to the Site Planning Guide.
AC Mains Supply
Connect the system to a compatible AC mains supply as instructed in this guide.
WARNING! Electrical Shock Hazard. Use only qualified personnel for the
installation of all of the electrical supplies and fixtures, and make sure that
all of the installations adhere to local regulations and safety standards.
WARNING! Electrical Shock Hazard. Make sure that the system can be
disconnected from the mains supply outlet in an emergency. Do not block
the mains supply outlet.
WARNING! Electrical Shock Hazard. Use only the power cables supplied with
the system. Do not use power cables that are not properly rated for the
operation of this system.
Operator GuideC100HT Biologics Analyzer System
RUO-IDV-05-3828-A8 / 122
Operational Precautions and Limitations

Protective Earth Conductor
The mains supply must include a correctly installed protective earth conductor. The protective earth conductor
must be installed or checked by a qualified electrician before the system is connected.
WARNING! Electrical Shock Hazard. Do not intentionally interrupt the
protective earth conductor. Any interruption of the protective earth conductor
creates an electrical shock hazard.
Chemical Precautions
WARNING! Ionizing Radiation Hazard, Biohazard, or Toxic Chemical Hazard.
Determine whether decontamination is required prior to cleaning or
maintenance. The customer must decontaminate the system prior to cleaning
or maintenance if radioactive materials, biological agents, or toxic chemicals
have been used with the system.
WARNING! Environmental Hazard. Do not dispose of system components in municipal
waste. Follow local regulations when disposing of components.
•Determine which chemicals have been used in the system prior to service and regular maintenance. Refer to
the Safety Data Sheets for the health and safety precautions that must be followed with chemicals. SCIEX
Safety Data Sheets can be found at sciex.com/tech-regulatory.
•Always wear assigned personal protective equipment, including powder-free neoprene or nitrile gloves, safety
glasses, and a laboratory coat.
•Avoid ignition sources when working with flammable materials, such as isopropanol, methanol, and other
flammable solvents.
•Take care in the use and disposal of any chemicals. Potential risk of personal injury if proper procedures for
handling and disposing of chemicals are not followed.
•Avoid skin contact with chemicals during cleaning and wash hands after use.
•Collect all spent liquids and dispose of them as hazardous waste.
•Comply with all of the local regulations for the storage, handling, and disposal of biohazardous, toxic, or
radioactive materials.
C100HT Biologics Analyzer SystemOperator Guide
9 / 122RUO-IDV-05-3828-A
Operational Precautions and Limitations

Environmental Precautions
Use qualified personnel for the installation of electrical mains, heating, ventilation, and plumbing supplies and
fixtures. Make sure that all of the installations comply with local bylaws and biohazard regulations. For information
about the required environmental conditions for the system, refer to the Site Planning Guide.
Allow access space around the equipment when setting up the system.
WARNING! Biohazard. For biohazardous material use, always comply with local
regulations for hazard assessment, control, and handling. This system or any part
is not intended to act as a biological containment.
WARNING! Environmental Hazard. Follow established procedures for disposal
of biohazardous, toxic, radioactive, and electronic waste. The customer is
responsible for disposal of hazardous substances, including chemical, waste
oils and electrical components, in accordance with local laws and regulations.
Electromagnetic Environment
Electromagnetic Compatibility
Basic Electromagnetic Environment: Environment existing at locations characterized by being supplied
directly at low voltage from the public mains network.
The equipment is intended for use in a basic electromagnetic environment.
Make sure that a compatible electromagnetic environment for the equipment can be maintained so that the device
will perform as intended. If the power supply line is subject to high electrical noise, then install a surge protector.
Electromagnetic Interference
Class A Equipment: Equipment which is suitable for use in all establishments other than domestic and those
directly connected to a low voltage power supply network which supplies buildings used for domestic purposes.
[Derived from CISPR 11:2009, 5.3] Class A equipment shall meet Class A limits.
This equipment has been tested and found to comply with the limits for a Class A digital device, pursuant to Part
15 of the FCC (Federal Communications Commission) Compliance Rules.
These limits are designed to provide reasonable protection against harmful interference when the equipment is
operated in a commercial environment. This equipment generates, uses, and can radiate radio frequency energy
and, if not installed and used in accordance with the operator's manual, can cause harmful interference to radio
communications.
Operation of this equipment in a residential area is likely to cause harmful interference in which case you will be
required to correct the interference, at your own expense. Changes or modifications not expressly approved by
the manufacturer could void your authority to operate the equipment.
Operator GuideC100HT Biologics Analyzer System
RUO-IDV-05-3828-A10 / 122
Operational Precautions and Limitations

Decommissioning and Disposal
Before decommissioning, decontaminate the entire system following local regulations.
When removing the system from service, separate and recycle different materials according to national and local
environmental regulations.
Note: SCIEX will not accept any system returns without a completed Decontamination Form. Contact an FSE
to obtain a copy of the form.
Do not dispose of system components or subassemblies, including computer parts, as unsorted municipal waste.
Waste Electrical and Electronic Equipment
Follow local municipal waste ordinances for proper disposal provisions to reduce the environmental impact of
waste, electrical, and electronic equipment (WEEE). To safely dispose of this equipment, contact a local Customer
Service office for complimentary equipment pick-up and recycling.
Equipment Use and Modification
WARNING! Electrical Shock Hazard. Do not remove the covers. Removing the
covers might cause injury or malfunctioning of the system. The covers need
not be removed for routine maintenance, inspection, or adjustment. Contact
a SCIEX Field Service Employee (FSE) for repairs that require the covers to be
removed.
WARNING! Personal Injury Hazard. Use SCIEX-recommended parts only. Use of parts
not recommended by SCIEX or use of parts for any purpose other than their intended
purpose can put the user at risk of harm or negatively impact system performance.
WARNING! Lifting Hazard. Make sure that at least four people or a lifting device
are available to lift the LC system. Follow established safe lifting procedures. Refer
to the Site Planning Guide for the weights of system components.
WARNING! Crushing Hazard. Wear protective footwear when moving heavy objects.
Use the system indoors in a laboratory that complies with the environmental conditions recommended in the Site
Planning Guide.
If the system is used in an environment or in a manner not prescribed by the manufacturer, then the protection
provided by the equipment might be impaired.
C100HT Biologics Analyzer SystemOperator Guide
11 / 122RUO-IDV-05-3828-A
Operational Precautions and Limitations

Unauthorized modification or operation of the system might cause personal injury and equipment damage, and
might void the warranty. Erroneous data might be generated if the system is operated either above or below the
recommended environmental conditions or operated with unauthorized modifications. Contact an FSE for information
on servicing the system.
Qualified Personnel
Only qualified SCIEX personnel shall install, inspect, and service the equipment. After installing the system, the
Field Service Employee (FSE) uses the Installation Qualification to orient the customer on system operation,
cleaning, and basic maintenance.
Contact Us
SCIEX Support
•sciex.com/contact-us
•sciex.com/request-support
Customer Training
•In North America: [email protected]
•In Europe: [email protected]
•Outside the EU and North America, visit sciex.com/education for contact information.
Online Learning Center
•SCIEXUniversity
CyberSecurity
For the latest guidance on cybersecurity for SCIEX products, visit sciex.com/productsecurity.
Technical Support
SCIEX and its representatives maintain a staff of fully-trained service and technical specialists located throughout
the world. They can answer questions about the system or any technical issues that might arise. For more
information, visit the SCIEX website at sciex.com.
Operator GuideC100HT Biologics Analyzer System
RUO-IDV-05-3828-A12 / 122
Operational Precautions and Limitations

Documentation Symbols and Conventions
The following symbols and conventions are used throughout the guide.
DANGER! Danger signifies an action which leads to severe injury or death.
WARNING! Warning signifies an action that could cause personal injury if precautions
are not followed.
CAUTION: Caution signifies an operation that could cause damage to the system or
corruption or loss of data if precautions are not followed.
Note: Note emphasizes significant information in a procedure or description.
Tip! Tip provides useful information that helps apply the techniques and procedures in the text for a specific
need and provides shortcuts, but is not essential to the completion of a procedure.
C100HT Biologics Analyzer SystemOperator Guide
13 / 122RUO-IDV-05-3828-A
Operational Precautions and Limitations

This guide describes the basic operation, troubleshooting, and maintenance for the C100HT Biologics Analyzer
system. Read this guide thoroughly before using the product and operate the product in accordance with the
instructions in this guide.
This guide provides safety instructions and precautions to make sure that the user operates the system safely.
Follow all Warning and Caution instructions provided in the guide.
Description
The C100HT Biologics Analyzer system is a high-throughput capillary electrophoresis system capable of performing
glycan separation for up to 96 samples. After the separation is complete, the results are analyzed to identify
samples that differ from a reference sample. The results can be viewed with the DataReviewer software and the
user can change the criteria for matching.
The system includes the following components:
•The C100HT Biologics Analyzer capillary electrophoresis system
•A SCIEX-supplied controller consisting of a computer and a monitor. Pre-installed on the controller are:
•C100HT software
•DataReviewer software
The C100HT Biologics Analyzer system is designed to work with the C100HT Glycan Labeling and Analysis kit and
cartridge.
Operator GuideC100HT Biologics Analyzer System
RUO-IDV-05-3828-A14 / 122
2
Introduction

Hardware Overview
Figure 2-1 Front and Side Panel
DescriptionItem
Service door1
Cartridge door2
Sample door3
LED power indicator; green when the system is on, blue when it is off.4
C100HT Biologics Analyzer SystemOperator Guide
15 / 122RUO-IDV-05-3828-A
Introduction

Figure 2-2 Back Panel
DescriptionItem
Name plate1
FCC Compliance label2
Waste Electrical and Electronic Equipment label3
No User-serviceable Parts label4
For Research Use only label5
RoHS label6
RS-232 connector7
Voltage switch8
Power switch, mains supply connection, and fuse holder9
High Voltage label10
Operator GuideC100HT Biologics Analyzer System
RUO-IDV-05-3828-A16 / 122
Introduction

Cartridge
Note: Always allow the cartridge to stand in the cartridge stand at room temperature for at least 20 minutes
before use.
Figure 2-3 Cartridge in Cartridge Stand
DescriptionItem
Gel reservoir1
Cartridge purge port2
Cartridge data key3
Capillary windows4
Cartridge stand5
C100HT Biologics Analyzer SystemOperator Guide
17 / 122RUO-IDV-05-3828-A
Introduction

Theory of Operation
The C100HT Biologics Analyzer system uses capillary electrophoresis to enable high resolution and sensitive
separation of glycan samples. This 12-channel capillary electrophoresis instrument uses multiple-use cartridges
for cost-effective analysis of up to 96 samples in as little as 2 hours and 50 minutes. The cartridge can be used for
1000 separations.
1. The C100HT Biologics Analyzer system is set up with a gel cartridge and running and wash buffers.
The cartridge is normalized using intensity markers.
2. The following steps are performed to prepare the samples:
a. Enzymatic release of the N-glycans from glycoproteins.
b. Labeling the released glycans with a charged fluorophore.
c. Magnetic bead-mediated capture of the fluorophore-labeled glycans and dye removal, followed by release
of the labeled glycans.
3. The samples for analysis (in a 96-well plate or 12-tube strips) are placed in the sample plate holder.
4. The user selects the parameters for data acquisition and then initiates the run in the C100HT software.
5. The sample is injected using an electric field generated by the power supply.
6. During the run, the electric field causes samples to migrate from the sample tray through the capillary. Eventually
the sample molecules pass a window in the cartridge. The user can monitor the run in the Gel Image and
Electropherogram tabs in the C100HT software. The samples are separated due to differences in mass to charge
ratio, and differential interaction with the gel.
7. The system detects the dye-labeled glycans with LED-induced fluorescence, with excitation at 450 nm and
emission at 540 nm. The C100HT software saves the fluorescence data to an experiment file.
8. The resulting experiment file is analyzed and the results are displayed in the DataReviewer software, where
the user can adjust the parameters used to identify glycans and abundance. The DataReviewer software creates
two reports that can be printed or saved.
Operator GuideC100HT Biologics Analyzer System
RUO-IDV-05-3828-A18 / 122
Introduction

Safety
Refer to the Safety Data Sheets (SDS), available at sciex.com/tech-regulatory, for information about the proper
handling of materials and reagents. Always follow standard laboratory safety guidelines.
Overview
The SCIEX C100HT Glycan Labeling and Analysis kit provides reagents and supplies required to label, separate,
and identify reducing carbohydrates for 1000 samples using the C100HT Biologics Analyzer System. This is a widely
applicable sample preparation and analysis method for N-glycan profiling of glycoproteins. Carbohydrate profiling
of therapeutic glycoproteins provides valuable data toward understanding the activity and efficacy of these
molecules. Glycans strongly influence circulation half-life, immunogenicity, and receptor-binding activity as well
as physicochemical and thermal stability of proteins of therapeutic interest.
The protocol consists of the following steps:
1. Enzymatic release of the N-glycans from glycoproteins.
2. Magnetic bead-mediated capture of the released glycans.
3. Labeling the released glycans with a charged fluorophore.
4. Magnetic bead-mediated capture of the fluorophore-labeled glycans and dye removal, followed by release of
the labeled glycans.
5. Separation of the labeled glycans by capillary electrophoresis with detection of LED-induced fluorescence.
6. Analysis of the capillary electrophoresis separation results to identify the type of glycans in the samples followed
by review of the results with the DataReviewer software.
Intended Use
The C100HT Glycan Labeling and Analysis kit is for laboratory use only.
C100HT Biologics Analyzer SystemOperator Guide
19 / 122RUO-IDV-05-3828-A
3
Prepare the Samples

Equipment and Materials Required
Note: Unless a manufacturer name is specified, part numbers are for items available from SCIEX.
For information about the contents of the kit, refer to C100HT Glycan Labeling and Analysis Kit.
Customer-Supplied Equipment and Supplies
•Powder-free gloves (neoprene or nitrile recommended)
•Safety glasses
•Laboratory coat
•96-well plates (PN 609801)
•Magnetic plate
•For automated sample preparation: Magnum EX Universal Magnet Plate (Alpaqua PN A000380) (or
equivalent)
•For manual sample preparation: Magnetic bead separation block for 96-well plates with 24 post 52 MGO
magnets (VP Scientific PN VP771LE-4DS) (or equivalent)
•Orbital shaker capable of 1500 rpm or equivalent, with 96-well plate adapter
•Luer-Lok syringe (or equivalent) with a 22 gauge needle
•Heat block capable of maintaining 60 °C
•Vortex mixer
•Thermometer for measuring the heat block temperature
•Pipettes (2 μL, 10 μL, 20 μL, 100 μL or 200 μL, and 1 mL) and appropriate tips
•Multi-channel pipettes (20 µL and 200 µL) and appropriate tips
•Miscellaneous lab glassware for buffers and reagents
Customer-Supplied Reagents
•Peptide-N-glycosidase F enzyme (PNGase F), either:
•200 mU (Prozyme PN GKE5006B) (enough for approximately 40 samples)
•75 000 units, glycerol-free (New England Biolabs PN P0705L) (enough for approximately 100 samples)
•HPLC-grade acetonitrile
•1 M sodium cyanoborohydride in THF (Sigma-Aldrich PN 296813)
Operator GuideC100HT Biologics Analyzer System
RUO-IDV-05-3828-A20 / 122
Prepare the Samples