SCIEX NanoSpray Manual

NanoSpray®Ion Source
Operator Guide
May 2018RUO-IDV-05-0811-F

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1 Operational Precautions and Limitations...............................................................................................6
Operational Precautions and Hazards................................................................................................................................6
Chemical Precautions.........................................................................................................................................................7
System Safe Fluids.......................................................................................................................................................8
Laboratory Conditions........................................................................................................................................................9
Operating Conditions...................................................................................................................................................9
Performance Specifications..........................................................................................................................................9
Equipment Use and Modification.....................................................................................................................................10
Contact Us.......................................................................................................................................................................10
Related Documentation...................................................................................................................................................11
Technical Support............................................................................................................................................................11
Laser Safety Information..................................................................................................................................................11
Laser Classification....................................................................................................................................................11
Scheduled Maintenance.............................................................................................................................................12
Specifications.............................................................................................................................................................12
Labels on the Ion Source............................................................................................................................................12
2 Ion Source Overview..............................................................................................................................14
3 Ion Source Components.........................................................................................................................15
Camera and Illuminator...................................................................................................................................................16
Ion Source Head...............................................................................................................................................................18
X-Y-Z Positioning Unit.....................................................................................................................................................18
Positioning Rails..............................................................................................................................................................19
NanoSpray®Interface Components.................................................................................................................................20
OptiFlowTM Interface Components...................................................................................................................................20
Requirements...................................................................................................................................................................22
Gases.........................................................................................................................................................................22
Solvents.....................................................................................................................................................................22
4 NanoSpray®III Head Assembly and Installation..................................................................................23
Assemble the NanoSpray®III Head..................................................................................................................................24
Remove the NanoSpray®III Head....................................................................................................................................26
Prepare the Sprayer Fitting..............................................................................................................................................28
Insert the Emitter Tip.......................................................................................................................................................30
Install the NanoSpray III Head on the Bracket.................................................................................................................31
Connect the Sample Line.................................................................................................................................................32
Adjust the Illuminator and Camera..................................................................................................................................34
Adjust and Focus the Camera....................................................................................................................................34
Adjust the Illuminator................................................................................................................................................36
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Contents

Inspect for Leaks..............................................................................................................................................................40
5 Optimize the NanoSpray III Head.........................................................................................................42
6 Ion Source Maintenance........................................................................................................................44
Remove the Ion Source....................................................................................................................................................45
Install the Ion Source.......................................................................................................................................................46
Change Ion Sources.........................................................................................................................................................48
Change to a Different Ion Source (OptiFlowTM Interface Components)......................................................................49
Change to the NanoSpray®Ion Source (OptiFlowTM Interface
Components).............................................................................................................................................................49
Change to a Different Ion Source (NanoSpray®Interface Components)....................................................................49
Change to the NanoSpray®Ion Source (NanoSpray®Interface
Components).............................................................................................................................................................50
Change the Interface Components...................................................................................................................................50
Install the OptiFlowTM Interface Components............................................................................................................50
Remove the Interface Components............................................................................................................................52
Install the Interface Components...............................................................................................................................54
Remove the Monitor........................................................................................................................................................56
Remove the Monitor from the 4500, 5500, 6500, 6500+, and TripleTOF®
Systems......................................................................................................................................................................56
Remove the Monitor from the 3200 and 4000 Series Systems...................................................................................57
Install the Monitor...........................................................................................................................................................57
Install the Monitor 4500, 5500, 6500, 6500+, and TripleTOF®Systems.....................................................................57
Install the Monitor on 3200 and 4000 Series Systems...............................................................................................59
Connect the Monitor Cables............................................................................................................................................60
Bake the Interface............................................................................................................................................................61
Clean the Ion Source........................................................................................................................................................62
Clean the Nano Cell Heater Assembly..............................................................................................................................62
Required Materials ....................................................................................................................................................62
Tools and Supplies Available from the Manufacturer.................................................................................................63
Clean the Assembly....................................................................................................................................................64
7 Troubleshooting.....................................................................................................................................66
Mass Spectrometer Troubleshooting Tips........................................................................................................................66
Syringe Troubleshooting Tips...........................................................................................................................................67
External Pump Troubleshooting Tips................................................................................................................................67
NanoSpray®III Head Troubleshooting Tips......................................................................................................................68
Spray Plume Troubleshooting Tips...................................................................................................................................73
Monitor and Camera Troubleshooting Tips......................................................................................................................73
Emitter Tip Troubleshooting Tips.....................................................................................................................................74
Acquisition Troubleshooting Tips.....................................................................................................................................75
A Principles of Operation.........................................................................................................................78
B Tips for Working with the Ion Source..................................................................................................80
Cleaving an Emitter Tip....................................................................................................................................................80
NanoSpray®III Head........................................................................................................................................................80
Sample Solvent Composition.....................................................................................................................................80
Factors Affecting Optimization..................................................................................................................................80
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Contents

C Connecting the Syringe Using PEEK-lined Fused Silica Tubing..........................................................83
D Source Parameters and Voltages..........................................................................................................87
E Glossary of Symbols...............................................................................................................................88
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Contents

Note: Before operating the system, carefully read all of the sections of this guide.
This section contains general safety-related information. It also describes potential hazards and associated warnings
for the system and the precautions that should be taken to minimize the hazards.
In addition to this section, refer to Glossary of Symbols for information about the symbols and conventions used
in the laboratory environment, on the system, and in this documentation.
Operational Precautions and Hazards
For regulatory and safety information for the mass spectrometer, refer to the safety guide or System User Guide.
WARNING! Ionizing Radiation Hazard, Biohazard, or Toxic Chemical Hazard.
Use the ion source only if you have knowledge of and training in the proper
use, containment, and evacuation of toxic or injurious materials used with
the ion source.
WARNING! Hot Surface Hazard. Let the NanoSpray®ion source cool for at
least 60 minutes before starting any maintenance procedures. Surfaces of
the ion source become hot during operation.
WARNING! Toxic Chemical Hazard. Wear personal protective equipment, including
a laboratory coat, gloves, and safety glasses, to avoid skin or eye exposure.
WARNING! Ionizing Radiation Hazard, Biohazard, or Toxic Chemical Hazard.
In the event of a chemical spill, review product Safety Data Sheets for specific
instructions. Use appropriate personal protective equipment and absorbent
wipes to contain the spill and dispose of it following local regulations.
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1
Operational Precautions and
Limitations

WARNING! Environmental Hazard. Do not dispose of system components in municipal
waste. Follow local regulations when disposing of components.
WARNING! Electrical Shock Hazard. Avoid contact with the high voltages
applied to the ion source during operation. Put the system in Standby mode
before adjusting the sample tubing or other equipment near the ion source.
Chemical Precautions
WARNING! Ionizing Radiation Hazard, Biohazard, or Toxic Chemical Hazard.
Determine whether decontamination is required prior to cleaning or
maintenance. The customer must decontaminate the system prior to cleaning
or maintenance if radioactive materials, biological agents, or toxic chemicals
have been used with the system.
WARNING! Environmental Hazard. Do not dispose of system components in municipal
waste. Follow local regulations when disposing of components.
WARNING! Biohazard, Toxic Chemical Hazard. Connect the drain tubing to
the mass spectrometer and the source exhaust drain bottle properly, to
prevent leaks.
•Determine which chemicals have been used in the system prior to service and regular maintenance. Refer to
the Safety Data Sheets for the health and safety precautions that must be followed with chemicals. SCIEX
Safety Data Sheets can be found at sciex.com/tech-regulatory.
•Always wear assigned personal protective equipment, including powder-free neoprene or nitrile gloves, safety
glasses, and a laboratory coat.
•Avoid ignition sources when working with flammable materials, such as isopropanol, methanol, and other
flammable solvents.
•Take care in the use and disposal of any chemicals. Potential risk of personal injury if proper procedures for
handling and disposing of chemicals are not followed.
•Avoid skin contact with chemicals during cleaning and wash hands after use.
•Make sure that all exhaust hoses are connected properly and that all connections are functioning as designed.
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Operational Precautions and Limitations

•Collect all spent liquids and dispose of them as hazardous waste.
•Comply with all of the local regulations for the storage, handling, and disposal of biohazardous, toxic, or
radioactive materials.
•(Recommended) Use secondary containment trays beneath the roughing pump, the solvent bottles, and the
waste collection container to capture potential chemical spills.
System Safe Fluids
The following fluids can safely be used with the system.
CAUTION: Potential System Damage. Do not use any other fluid until confirmation is
received from SCIEX that it does not present a hazard. This is not an exhaustive list.
•Organic Solvents
•MS-grade acetonitrile; up to 100%
•MS-grade methanol; up to 100%
•Isopropanol; up to 100%
•HPLC-grade or higher water; up to 100%
•Tetrahydrofuran; up to 100%
•Toluene and other aromatic solvents; up to 100%
•Hexanes; up to 100%
•Buffers
•Ammonium acetate; less than 1%
•Ammonium formate; less than 1%
•Phosphate; less than 1%
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Operational Precautions and Limitations

•Acids and Bases
•Formic acid; less than 1%
•Acetic acid; less than 1%
•Trifluoroacetic acid (TFA); less than 1%
•Heptafluorobutyric acid (HFBA); less than 1%
•Ammonia/ammonium hydroxide; less than 1%
•Phosphoric acid; less than 1%
•Trimethylamine; less than 1%
•Triethylamine; less than 1%
Laboratory Conditions
Operating Conditions
The system is designed to operate safely under these conditions:
•Indoors
•Altitude: Up to 2 000 m (6 560 feet) above sea level
•Ambient temperature: 5 °C (41 °F) to 40 °C (104 °F)
•Relative humidity: 80% for temperatures up to 31 °C (88 °F), decreasing lineraly to 50% at 40 °C (104 °F)
•Mains supply voltage fluctuations: ±10% of the nominal voltage
•Transient overvoltages: Up to the levels of Overvoltage Category II
•Temporary overvoltages on the mains supply
•Pollution degree: Pollution Degree 2
•External power supply rating: 90 VAC to 264 VAC, 47 Hz to 63 Hz, 1 A
Performance Specifications
The system is designed to meet specifications under these conditions:
•An ambient temperature of 15 °C to 30 °C (59 °F to 86 °F)
Over time, the temperature must remain within a range of 4 °C (7.2 °F), with the rate of the change in
temperature not exceeding 2°C (3.6°F) per hour. Ambient temperature fluctuations exceeding the limits might
result in mass shifts in spectra.
•Relative humidity from 20% to 80%, non-condensing
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Operational Precautions and Limitations

Equipment Use and Modification
WARNING! Personal Injury Hazard. Contact the SCIEX representative if product
installation, adjustment, or relocation is required.
WARNING! Electrical Shock Hazard. Do not remove the covers. Removing the
covers might cause injury or malfunctioning of the system. The covers need
not be removed for routine maintenance, inspection, or adjustment. Contact
a SCIEX Field Service Employee (FSE) for repairs that require the covers to be
removed.
WARNING! Personal Injury Hazard. Use SCIEX-recommended parts only. Use of parts
not recommended by SCIEX or use of parts for any purpose other than their intended
purpose can put the user at risk of harm or negatively impact system performance.
Use the mass spectrometer and ion source indoors in a laboratory that complies with the environmental conditions
recommended in the Site Planning Guide.
If the mass spectrometer and ion source are used in an environment or in a manner not prescribed by the
manufacturer, then the protection provided by the equipment might be impaired.
Unauthorized modification or operation of the mass spectrometer and ion source might cause personal injury and
equipment damage, and might void the warranty. Erroneous data might be generated if the mass spectrometer
and ion source is operated either above or below the recommended environmental conditions or operated with
unauthorized modifications. Contact an FSE for information on servicing the system.
Contact Us
SCIEX Support
•sciex.com/contact-us
•sciex.com/request-support
Customer Training
•In North America: [email protected]
•In Europe: [email protected]
•Outside the EU and North America, visit sciex.com/education for contact information.
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Operational Precautions and Limitations