SCIEX 6500 series User manual

6500 and 6500+ Series of Instruments
System User Guide
August 2015RUO-IDV-05-2095-A

This document is provided to customers who have purchased SCIEX equipment to use in the operation of such SCIEX
equipment. This document is copyright protected and any reproduction of this document or any part of this document is
strictly prohibited, except as SCIEX may authorize in writing.
Software that may be described in this document is furnished under a license agreement. It is against the law to copy, modify,
or distribute the software on any medium, except as specifically allowed in the license agreement. Furthermore, the license
agreement may prohibit the software from being disassembled, reverse engineered, or decompiled for any purpose. Warranties
are as stated therein.
Portions of this document may make reference to other manufacturers and/or their products, which may contain parts whose
names are registered as trademarks and/or function as trademarks of their respective owners. Any such use is intended only
to designate those manufacturers' products as supplied by SCIEX for incorporation into its equipment and does not imply
any right and/or license to use or permit others to use such manufacturers' and/or their product names as trademarks.
SCIEX warranties are limited to those express warranties provided at the time of sale or license of its products and are SCIEX’s
sole and exclusive representations, warranties, and obligations. SCIEX makes no other warranty of any kind whatsoever,
expressed or implied, including without limitation, warranties of merchantability or fitness for a particular purpose, whether
arising from a statute or otherwise in law or from a course of dealing or usage of trade, all of which are expressly disclaimed,
and assumes no responsibility or contingent liability, including indirect or consequential damages, for any use by the purchaser
or for any adverse circumstances arising therefrom.
For research use only. Not for use in diagnostic procedures.
AB Sciex is doing business as SCIEX.
The trademarks mentioned herein are the property of AB Sciex Pte. Ltd. or their respective owners.
AB SCIEX™is being used under license.
©2015 AB SCIEX
AB Sciex Pte. Ltd.
Blk 33, #04-06
Marsiling Ind Estate Road 3
Woodlands Central Indus. Estate.
SINGAPORE 739256
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Chapter 1 Operational Precautions and Limitations................................................................................8
General Safety Information................................................................................................................................................8
Regulatory Compliance......................................................................................................................................................8
Australia and New Zealand..........................................................................................................................................8
Canada........................................................................................................................................................................9
Europe.........................................................................................................................................................................9
United States...............................................................................................................................................................9
International..............................................................................................................................................................10
Electrical Precautions.......................................................................................................................................................10
AC Mains Supply........................................................................................................................................................10
Protective Earth Conductor........................................................................................................................................11
Chemical Precautions.......................................................................................................................................................11
System Safe Fluids.....................................................................................................................................................12
Ventilation Precautions....................................................................................................................................................13
Environmental Precautions..............................................................................................................................................14
Electromagnetic Environment....................................................................................................................................15
Decommissioning and Disposal..................................................................................................................................15
Qualified Personnel..........................................................................................................................................................16
Equipment Use and Modification.....................................................................................................................................16
Contact Us.......................................................................................................................................................................17
Technical Support............................................................................................................................................................17
Related Documentation...................................................................................................................................................17
Chapter 2 Hazard Symbols.......................................................................................................................18
Occupational Health and Safety Symbols.........................................................................................................................18
Symbols, Indicators, and Labels: Packaging.....................................................................................................................20
Symbols, Indicators, and Labels: Mass Spectrometer.......................................................................................................22
Documentation Symbols and Conventions.......................................................................................................................23
Chapter 3 Principles of Operation............................................................................................................24
System Overview..............................................................................................................................................................24
Hardware Overview...................................................................................................................................................25
Theory of Operation—Hardware ..............................................................................................................................28
Analyst Software Overview .......................................................................................................................................30
Theory of Operation—Analyst Software....................................................................................................................38
Chapter 4 Operating Instructions —Sample Workflows.......................................................................41
Chapter 5 Operating Instructions—Hardware .......................................................................................44
Start Up the System.........................................................................................................................................................44
Reset the Mass Spectrometer...........................................................................................................................................46
Shut Down the System.....................................................................................................................................................46
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Plumb the Mass Spectrometer ........................................................................................................................................47
Adjust the Integrated Syringe Pump Position.............................................................................................................47
Plumb the Diverter Valve in Injector Mode................................................................................................................50
Plumb the Diverter Valve in Diverter Mode................................................................................................................52
Chapter 6 Operating Instructions —Hardware Profiles and Projects...................................................54
Hardware Profiles............................................................................................................................................................54
Create a Hardware Profile..........................................................................................................................................54
Add Devices to a Hardware Profile............................................................................................................................58
Troubleshoot Hardware Profile Activation.................................................................................................................59
Projects and Subprojects..................................................................................................................................................60
Create Projects and Subprojects.................................................................................................................................60
Create Subprojects.....................................................................................................................................................61
Copy Subprojects.......................................................................................................................................................61
Switch Between Projects and Subprojects..................................................................................................................61
Installed Project Folders.............................................................................................................................................62
Back Up the API Instrument Folder............................................................................................................................63
Recover the API Instrument Folder.............................................................................................................................63
Chapter 7 Operating Instructions —Tune and Calibrate.......................................................................64
Optimize the Mass Spectrometer.....................................................................................................................................64
About the Verifying or Adjusting Performance Dialog.....................................................................................................65
Results Summary..............................................................................................................................................................65
Chapter 8 Operating Instructions —Automatic Optimization..............................................................67
About Automatic Optimization........................................................................................................................................67
Types of Sample Introduction.....................................................................................................................................68
Automatically Optimize for an Analyte Using Infusion.....................................................................................................68
Confirm the Presence of Compounds.........................................................................................................................68
Perform Automatic MS and MS/MS Optimization Using Infusion with a
Precursor Ion and an Unknown Product Ion...............................................................................................................71
Check the Optimization Results.................................................................................................................................73
Automatically Optimize for an Analyte Using FIA............................................................................................................74
Chapter 9 Operating Instructions —Acquisition Methods....................................................................81
Create an Acquisition Method Using the Acquisition Method Editor...............................................................................81
Configure the Syringe Pump......................................................................................................................................82
Add an Experiment....................................................................................................................................................82
Add a Period..............................................................................................................................................................82
Copy an Experiment into a Period..............................................................................................................................82
Copy an Experiment within a Period..........................................................................................................................83
Scan Techniques..............................................................................................................................................................83
Quadrupole-Mode Scan Types...................................................................................................................................83
LIT-Mode Scan Types.................................................................................................................................................84
About Spectral Data Acquisition......................................................................................................................................85
Chapter 10 Operating Instructions —Batches .......................................................................................86
Set Queue Options...........................................................................................................................................................86
Add Sets and Samples to a Batch....................................................................................................................................87
Submit a Sample or Set of Samples..................................................................................................................................90
Change Sample Order......................................................................................................................................................91
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Acquire Data....................................................................................................................................................................91
Set Sample Locations in the Batch Editor.........................................................................................................................91
Select Vial Positions Using the Locations Tab (Optional).................................................................................................92
Set Quantitation Details in the Batch Editor (Optional)....................................................................................................93
Stop Sample Acquisition..................................................................................................................................................94
Import and Submit Batch Files.........................................................................................................................................94
Build a Batch as a Text File........................................................................................................................................94
Import a Batch as a Text File......................................................................................................................................95
Batch Editor Right-click Menu..........................................................................................................................................95
Queue States and Device Status......................................................................................................................................96
Queue States..............................................................................................................................................................96
View Instrument and Device Status Icons..................................................................................................................97
Queue Right-click Menu.............................................................................................................................................98
Chapter 11 Operating Instructions —Analyze and Process Quantitative Data.................................100
Quantitative Analysis.....................................................................................................................................................100
Quantitation Methods..............................................................................................................................................100
About Results Tables...............................................................................................................................................101
Quantitation Methods and Results Tables...............................................................................................................101
Create a Method Using the Quantitation Method Editor.........................................................................................101
Create a Results Table Using the Quantitation Wizard............................................................................................103
Create a Standard Query..........................................................................................................................................104
Results Table Right-click Menu................................................................................................................................106
Peak Review and Manual Integration of Peaks..............................................................................................................107
Review Peaks...........................................................................................................................................................107
Manually Integrate Peaks........................................................................................................................................111
Peak Review Right-Click Menu................................................................................................................................112
Calibration Curves.........................................................................................................................................................112
View Calibration Curves...........................................................................................................................................113
Overlay Calibration Curves.......................................................................................................................................114
Calibration Curve Right-Click Menu.........................................................................................................................114
Sample Statistics............................................................................................................................................................115
View the Statistics for Standards and QCs...............................................................................................................115
Compare Results Between Batches................................................................................................................................116
Chapter 12 Operating Instructions —Analyze and Process Data........................................................117
Open Data Files..............................................................................................................................................................117
Navigate Between Samples in a Data File......................................................................................................................117
Show Experimental Conditions......................................................................................................................................118
Show Data in Tables......................................................................................................................................................119
Show ADC Data.............................................................................................................................................................121
Show Basic Quantitative Data........................................................................................................................................121
Chromatograms.............................................................................................................................................................122
Show TICs from a Spectrum...........................................................................................................................................123
Show a Spectrum from a TIC..........................................................................................................................................123
About Generating XICs..................................................................................................................................................123
Generate an XIC Using a Selected Range.................................................................................................................124
Generate an XIC Using the Maximum Peak.............................................................................................................124
Generate an XIC Using Base Peak Masses...............................................................................................................124
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Extract Ion by Selecting Masses...............................................................................................................................125
Generate BPCs...............................................................................................................................................................126
Generate XWCs..............................................................................................................................................................127
Generate DAD Data.......................................................................................................................................................127
Generate TWCs..............................................................................................................................................................128
Adjust the Threshold......................................................................................................................................................128
Chromatogram Panes....................................................................................................................................................129
Spectra Panes................................................................................................................................................................130
Data Processing.............................................................................................................................................................130
Graphs...........................................................................................................................................................................131
Manage Data...........................................................................................................................................................131
Zoom In on the Y-axis..............................................................................................................................................133
Zoom In on the X-axis..............................................................................................................................................133
Chapter 13 Reporter Software...............................................................................................................134
Reporter User Interface..................................................................................................................................................135
Generate Reports...........................................................................................................................................................137
Chapter 14 Service and Maintenance Information...............................................................................138
Recommended Maintenance Schedule..........................................................................................................................138
Clean the Surfaces.........................................................................................................................................................140
Clean the Front-End.......................................................................................................................................................140
Symptoms of Contamination....................................................................................................................................140
Required Materials...................................................................................................................................................141
Best Practices...........................................................................................................................................................142
Prepare the Mass Spectrometer...............................................................................................................................143
Clean the Curtain Plate............................................................................................................................................144
Clean the Front of the Orifice Plate..........................................................................................................................145
Put the Mass Spectrometer Back in Service.............................................................................................................145
Empty the Source Exhaust Drain Bottle..........................................................................................................................145
Storage and Handling ...................................................................................................................................................148
Chapter 15 Mass Spectrometer Troubleshooting.................................................................................149
Appendix A Operating Instructions —Manual Optimization.............................................................150
About Manual Compound Optimization........................................................................................................................151
About Scan Types...........................................................................................................................................................151
Manually Optimize an Analyte.......................................................................................................................................151
Confirm the Presence of Compounds.......................................................................................................................152
Optimize MS-Specific Parameters............................................................................................................................153
Determine the Product Ions for Optimization...........................................................................................................155
Optimize Collision Cell Exit Potential for each Product Ion......................................................................................156
Manually Optimize the Ion Source and Gas Parameters................................................................................................157
Prepare the Ion Source.............................................................................................................................................157
Optimize the Ion Source Parameters........................................................................................................................157
Appendix B 6500 and 6500+ Series System Parameters.......................................................................159
Appendix C Calibration Ions and Solutions..........................................................................................163
Appendix D Toolbar Icons......................................................................................................................165
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Revision History......................................................................................................................................173
Index........................................................................................................................................................174
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Note: Before operating the system, carefully read all the sections of this guide.
This section contains general safety-related information and provides regulatory compliance information. It also
describes potential hazards and associated warnings for the system and the precautions that should be taken to
minimize the hazards.
In addition to this section, refer to Hazard Symbols on page 18 for information about the symbols and conventions
used in the laboratory environment, on the system, and in this documentation. Refer to the Site Planning Guide
for site requirements, including AC mains supply, source exhaust, ventilation, compressed air, nitrogen, and
roughing pump requirements.
General Safety Information
To prevent personal injury or system damage, read, understand, and obey all of the safety precautions, warnings
in this document, and labels on the mass spectrometer. These labels are shown with internationally recognized
symbols. Failure to heed these warnings could result in serious injury.
This safety information is intended to supplement federal, state, provincial, and local environmental health and
safety (EHS) regulations. The information provided covers system-related safety with regard to the operation of
the mass spectrometer. It does not cover every safety procedure that should be practised. Ultimately, the user and
the organization are responsible for compliance with federal, state, provincial, and local EHS regulations and for
maintaining a safe laboratory environment.
Refer to the appropriate laboratory reference material and standard operating procedures.
Regulatory Compliance
This system complies with the regulations and standards listed in this section. Refer to the Declaration of Conformity
included with the system and the individual system components for dated references. Applicable labels have been
affixed to the system.
Australia and New Zealand
•Electromagnetic Compatibility (EMC): Radio Communications Act 1992 as implemented in these
standards:
•Electromagnetic Interference—AS/NZS CISPR 11/ EN 55011/ CISPR 11 (Class A)
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Limitations

•Safety: AS/NZ 61010-1 and IEC 61010-2-061
Canada
•Electromagnetic Interference (EMI): CAN/CSAA CISPR11. This ISM device complies with Canadian
ICES-001.
•Safety: CAN/CSA C22.2 No. 61010-1 and CAN/CSA C22.2 No 61010-2-061
Europe
•Electromagnetic Compatibility (EMC): Electromagnetic Compatibility directive 2004/108/EC as
implemented in these standards:
•EN 61326-1
•EN 55011 (Class A)
•Safety: Low Voltage Directives 2006/95/EC as implemented in these standards:
•EN 61010-1
•EN 61010-2-061
•Waste Electrical and Electronic Equipment (WEEE): Waste Electrical and Electronic Equipment
2012/96/EEC, as implemented in EN 40519
•Packaging and Packaging Waste (PPW): Packaging and Packaging Waste Directive 94/62/EC
United States
•Radio Emissions Interference Regulations: 47 CFR 15 as implemented in this standard: FCC Part 15
(Class A)
•Safety: Occupational Safety and Health Regulations, 29 CFR 1910, as implemented by the following standards:
•UL 61010-1
•IEC 61010-2-061
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International
•Electromagnetic Compatibility (EMC):
•IEC 61326-1
•IEC CISPR 11 (Class A)
•IEC 61000-3-2
•IEC 61000-3-3
•Safety:
•IEC 61010-1
•IEC 61010-2-061
Electrical Precautions
WARNING! Electrical Shock Hazard. Do not remove the covers. Removing the covers
might cause injury or malfunctioning of the system. The covers need not be removed
for routine maintenance, inspection, or adjustment. Contact the SCIEX FSE for repairs
that require the covers to be removed.
For information on system electrical specifications, refer to the Site Planning Guide.
AC Mains Supply
WARNING! Electrical Shock Hazard. Use only qualified personnel for the installation
of all electrical supplies and fixtures, and make sure that all installations adhere to
local regulations and safety standards.
WARNING! Electrical Shock Hazard. Make sure that the system can be disconnected
from the AC mains supply outlet in an emergency. Do not block the AC mains supply
outlet.
CAUTION: Potential System Damage. Do not unpack the mass spectrometer crate or
computer boxes. The FSE will unpack and help move the mass spectrometer at the time of
the installation.
An external line transformer is not needed for the mass spectrometer, optional bench, or roughing pump.
Connect the system to a compatible AC mains supply as instructed in this guide.
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Protective Earth Conductor
The mains supply must include a correctly installed protective earth conductor. The protective earth conductor
must be installed or checked by a qualified electrician before the system is connected.
WARNING! Electrical Shock Hazard. Do not intentionally interrupt the protective
earth conductor. Any interruption of the protective earth conductor will create an
electrical shock hazard.
WARNING! Electrical Shock Hazard: Make sure that a protective earth (grounding
cable) is connected between the sample loop and an appropriate grounding point
at the mass spectrometer ion source. This supplementary grounding will reinforce
the safety configuration specified by SCIEX.
Chemical Precautions
WARNING! Radiation Hazard, Biohazard, or Toxic Chemical Hazard. Determine
whether mass spectrometer decontamination is required prior to cleaning or
maintenance. The customer must decontaminate the system prior to cleaning
or maintenance if radioactive materials, biological agents, or toxic chemicals
have been used with a mass spectrometer.
WARNING! Environmental Hazard. Do not dispose of system components in municipal
waste. Follow local regulations when disposing of components.
WARNING! Biohazard, Toxic Chemical Hazard. Connect the drain tubing to the
mass spectrometer and the source exhaust drain bottle properly, to prevent
leaks.
CAUTION: Potential System Damage. Do not submerge the end of the drain tubing in the
waste liquid in the waste container.
•Determine which chemicals have been used in the system prior to service and regular maintenance. Refer to
Safety Data Sheets for the health and safety precautions that must be followed with chemicals.
•Work in a well-ventilated area.
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•Always wear assigned personal protective equipment, including powder-free neoprene or nitrile gloves, safety
glasses, and a laboratory coat.
•Follow required electrical safe work practices.
•Avoid ignition sources when working with flammable materials, such as isopropanol, methanol, and other
flammable solvents.
•Take care in the use and disposal of any chemicals. Potential risk of personal injury if proper procedures for
handling and disposing of chemicals are not followed.
•Avoid skin contact with chemicals during cleaning, and wash hands after use.
•Comply with all local regulations for the storage, handling, and disposal of biohazardous, toxic, or radioactive
materials.
•(Recommended) Use secondary containment trays beneath the roughing pump, the solvent bottles, and the
waste collection container to capture potential chemical spills.
System Safe Fluids
The following fluids can safely be used with the system. Refer to Required Materials on page 141 for information
about safe cleaning solutions.
CAUTION: Potential System Damage. Do not use any other fluid until confirmation is
received from SCIEX that it will not present a hazard. This is not an exhaustive list.
•Organic Solvents
•MS-grade acetonitrile; up to 100%
•MS-grade methanol; up to 100%
•Isopropanol; up to 100%
•HPLC-grade or higher water; up to 100%
•Tetrahydrofuran; up to 100%
•Toluene and other aromatic solvents; up to 100%
•Hexanes; up to 100%
•Buffers
•Ammonium Acetate; less than 1%
•Ammonium Formate; less than 1%
•Phosphate; less than 1%
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•Acids and Bases
•Formic Acid; less than 1%
•Acetic Acid; less than 1%
•Trifluoroacetic Acid; (TFA) less than 1%
•Heptafluorobutyric Acid; (HFBA) less than 1%
•Ammonia/Ammonium Hydroxide; less than 1%
•Phosphoric Acid; less than 1%
•Trimethylamine; less than 1%
•Triethylamine; less than 1%
Ventilation Precautions
The venting of fumes and disposal of waste must comply with all federal, state, provincial, and local health and
safety regulations. Use the system indoors in a laboratory that complies with the environmental conditions
recommended in the Site Planning Guide for the system.
The mass spectrometer ion source exhaust system and roughing pump must be vented to a dedicated laboratory
fume hood or an external exhaust system, as recommended in the Site Planning Guide.
WARNING! Fire Hazard. Make sure that the source exhaust system is connected and
functioning to prevent flammable vapor from accumulating in the ion source.
WARNING! Radiation Hazard, Biohazard, or Toxic Chemical Hazard. Take care
to vent exhaust gases to a dedicated laboratory fume hood or exhaust system,
and make sure that the vent tubing is secured with clamps.
WARNING! Radiation Hazard, Biohazard, or Toxic Chemical Hazard. Be sure
to use the system in a well-ventilated laboratory environment in compliance
with local regulations and with appropriate air exchange for the work
performed.
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WARNING! Radiation Hazard, Biohazard, or Toxic Chemical Hazard. Do not
operate the mass spectrometer if the source exhaust drain and roughing pump
exhaust hoses are not properly connected to the laboratory ventilation system.
Perform a regular check of the exhaust tubing to make sure that there are no
leaks. The use of mass spectrometers without proper system ventilation might
constitute a health hazard, and might result in serious injury.
WARNING! Radiation Hazard, Biohazard, or Toxic Chemical Hazard. Use the
ion source only if you have knowledge of and training in the proper use,
containment, and evacuation of toxic or injurious materials used with the ion
source.
WARNING! Puncture Hazard, Radiation Hazard, Biohazard, or Toxic Chemical
Hazard. Discontinue use of the ion source if the ion source window is cracked
or broken and contact an SCIEX Field Service Employee. Any toxic or injurious
materials introduced into the equipment will be present in the ion source and
exhaust output. Dispose of sharps following established laboratory safety
procedures.
Environmental Precautions
Use qualified personnel for the installation of electrical mains, heating, ventilation, and plumbing supplies and
fixtures. Make sure that all the installations comply with local bylaws and biohazard regulations. For more
information about the required environmental conditions for the system, refer to the Site Planning Guide.
DANGER! Explosion Hazard. Do not operate the system in an environment containing
explosive gases. The system is not designed for operation in an explosive
environment.
WARNING! Biohazard. For biohazardous material use, always comply with local
regulations for hazard assessment, control, and handling. This system or any part is
not intended to act as a biological containment.
CAUTION: Potential Mass Shift. Maintain a stable ambient temperature. If the temperature
changes by more than 2 °C, then the resolution and mass calibration might be affected.
CAUTION: Potential System Damage. Avoid exposure to corrosive gas and excessive dust.
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Electromagnetic Environment
Electromagnetic Compatibility
Basic Electromagnetic Environment: Environment existing at locations characterized by being supplied
directly at low voltage from the public mains network.
Performance Criteria A (Criteria A): Equipment shall operate as intended with no degradation of performance
and no loss of function during or after test.
Performance Criteria B (Criteria B): Equipment may experience loss of function (one or more) during test
but shall operate as intended with some degradation of performance and functions self-recoverable after test.
Performance Criteria C (Criteria C): Equipment may experience loss of function (one or more) during test
but shall operate as intended with some degradation of performance and functions recoverable by operator after
test.
The equipment is intended for use in a basic electromagnetic environment.
The expected performance loss under the electromagnetic immunity conditions is less than 60% change in total
ion count (TIC).
Make sure that a compatible electromagnetic environment for the equipment can be maintained so that the device
will perform as intended.
Electromagnetic Interference
Class A Equipment: Equipment which is suitable for use in all establishments other than domestic and those
directly connected to a low voltage power supply network which supplies buildings used for domestic purposes.
[Derived from CISPR 11:2009, 5.3] Class A equipment shall meet Class A limits.
This equipment has been tested and found to comply with the limits for a Class A digital device, pursuant to Part
15 of the FCC (Federal Communications Commission) Compliance Rules.
These limits are designed to provide reasonable protection against harmful interference when the equipment is
operated in a commercial environment. This equipment generates, uses, and can radiate radio frequency energy
and, if not installed and used in accordance with the operator's manual, can cause harmful interference to radio
communications.
Operation of this equipment in a residential area is likely to cause harmful interference in which case you will be
required to correct the interference, at your own expense. Changes or modifications not expressly approved by
the manufacturer could void your authority to operate the equipment.
Decommissioning and Disposal
Before decommissioning, decontaminate the system following local regulations.
When removing the system from service, separate and recycle different materials according to national and local
environmental regulations.
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Do not dispose of system components or subassemblies, including computer parts, as unsorted municipal waste.
Note: SCIEX will not accept any system returns without a completed Decontamination Form. Contact an FSE
to obtain a copy of the form.
Qualified Personnel
Only qualified SCIEX personnel shall install and service the equipment. After installing the system, the Field Service
Employee (FSE) uses the Customer Familiarization Checklist to orient the customer on system operation,
cleaning, and basic maintenance.
Only personnel qualified by the manufacturer shall maintain the equipment. A laboratory designate can be
familiarized with the Qualified Maintenance Person (QMP) procedures during the installation.
Equipment Use and Modification
WARNING! Personal Injury Hazard. Contact the SCIEX representative if product
installation, adjustment, or relocation is required.
WARNING! Electrical Shock Hazard. Do not remove the covers. Removing the covers
might cause injury or malfunctioning of the system. The covers need not be removed
for routine maintenance, inspection, or adjustment. Contact the SCIEX FSE for repairs
that require the covers to be removed.
WARNING! Personal Injury Hazard. Use SCIEX-recommended parts only. Use of parts
not recommended by SCIEX or use of parts for any use other than their intended
purpose can place the user at risk of harm or negatively impact system performance.
Use the system indoors in a laboratory that complies with the environmental conditions recommended in the Site
Planning Guide.
If the system is used in an environment or in a manner not prescribed by the manufacturer, then the protection
provided by the equipment might be impaired.
Unauthorized modification or operation of the system might cause personal injury and equipment damage, and
might void the warranty. Erroneous data might be generated if the system is operated either above or below the
recommended environmental conditions or operated with unauthorized modifications. Contact an FSE for information
on servicing the system.
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Contact Us
SCIEX Support
•sciex.com/contact-us
•sciex.com/support/request-support
Customer Training
•In North America: [email protected]
•In Europe: [email protected]
•Outside the EU and North America, visit sciex.com/education for contact information.
Online Learning Center
•sciex.com/LearningPortal
Technical Support
SCIEX and its representatives maintain a staff of fully-trained service and technical specialists located throughout
the world. They can answer questions about the system or any technical issues that might arise. For more
information, visit the SCIEX Web site at sciex.com .
Related Documentation
The guides and tutorials for the Analyst®software are installed automatically with the software and are available
from the Start menu: All Programs > SCIEX > Analyst. A complete list of the available documentation can
be found in the Help. To view the Help, press F1.
Documentation for the mass spectrometer can be found on the Customer Reference DVD for the mass
spectrometer.
Documentation for the ion source can be found on the Customer Reference DVD for the ion source.
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This section lists the hazard symbols and conventions used in the laboratory environment, on the system, and in
the documentation.
Occupational Health and Safety Symbols
This section describes some occupational health and safety symbols found in the documentation and laboratory
environment.
Table 2-1 General Hazard Symbols
DescriptionSafety Symbol
Personal Injury Hazard
Lifting Hazard
Table 2-2 Chemical Hazard Symbols
DefinitionSafety Symbol
Biohazard
Explosion Hazard
Toxic Chemical Hazard
Fire Hazard
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Hazard Symbols

Table 2-3 Electrical Hazard Warning Symbols
DefinitionSafety Symbol
Electrical Shock Hazard
Table 2-4 Pressurized Gas Hazard Warning Symbols
DefinitionSafety Symbol
Pressurized Gas Hazard
Table 2-5 Mechanical Hazard Symbols
DefinitionSafety Symbol
Hot Surface Hazard
Puncture Hazard
Table 2-6 Radiation Hazard Symbols
DefinitionSafety Symbol
Ionizing Radiation Hazard
Laser Radiation Hazard
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Hazard Symbols

Symbols, Indicators, and Labels: Packaging
Table 2-7 Labels on the Mass Spectrometer Shipping Materials
DefinitionLabel/Symbol
Tilt Indicator
Indicates whether the container was tipped or mishandled.
Write on the Bill of Lading and inspect for damage. Any claims for
tipping require a notation.
or
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Hazard Symbols