SCIEX P/ACE MDQ Plus Installation guide

P/ACE™ MDQ Plus Capillary
Electrophoresis System
System Overview Guide
RUO-IDV-05-4901-A
January 2019

AB Sciex LLC
500 Old Connecticut Path
Framingham, Massachusetts 01701
USA
This document is provided to customers who have purchased SCIEX equipment to use in the
operation of such SCIEX equipment. This document is copyright protected and any reproduction of
this document or any part of this document is strictly prohibited, except as SCIEX may authorize in
writing.
Software that may be described in this document is furnished under a license agreement. It is
against the law to copy, modify, or distribute the software on any medium, except as specifically
allowed in the license agreement. Furthermore, the license agreement may prohibit the software
from being disassembled, reverse engineered, or decompiled for any purpose. Warranties are as
stated therein.
Portions of this document may make reference to other manufacturers and/or their products, which
may contain parts whose names are registered as trademarks and/or function as trademarks of their
respective owners. Any such use is intended only to designate those manufacturers' products as
supplied by SCIEX for incorporation into its equipment and does not imply any right and/or license to
use or permit others to use such manufacturers' and/or their product names as trademarks.
SCIEX warranties are limited to those express warranties provided at the time of sale or license of
its products and are SCIEX’s sole and exclusive representations, warranties, and obligations.
SCIEX makes no other warranty of any kind whatsoever, expressed or implied, including without
limitation, warranties of merchantability or fitness for a particular purpose, whether arising from a
statute or otherwise in law or from a course of dealing or usage of trade, all of which are expressly
disclaimed, and assumes no responsibility or contingent liability, including indirect or consequential
damages, for any use by the purchaser or for any adverse circumstances arising therefrom.
For Research Use Only. Not for use in Diagnostic Procedures.
AB Sciex is operating as SCIEX.
The trademarks mentioned herein are the property of AB Sciex Pte. Ltd. or their respective owners.
AB SCIEX™ is being used under license.
© 2019 AB Sciex
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Contents
Chapter 1 Safety, Notices and Labels . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7
Overview. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7
Alerts for Warning, Caution, Important, and Note . . . . . . . . . . . . . . . . . . . . . . . . . 8
Instrument Safety Precautions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9
Moving Parts or Sharp Objects . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 10
Electrical Safety . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 10
Laser Safety (for Optional Laser Device) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 10
Class 1 Laser Caution Label . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11
Chemical Precautions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11
Safety Symbols and Labels. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 12
High Voltage Electric Shock Risk Symbol. . . . . . . . . . . . . . . . . . . . . . . . . . . . 12
Attention Safety Symbol . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 12
Sharp Object Label . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 12
Fuse Warning Symbol. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 13
Cancer and Reproductive Harm Label . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 13
RoHS Notices . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 13
China RoHS Caution Label. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 13
Other Instrument Labels . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 14
Recycling Label (WEEE) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 14
Disposal of Devices Containing Mercury Components . . . . . . . . . . . . . . . . . . 14
CE Mark Label . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 15
RCM Mark Label . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 15
CSA Mark Label . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 15
Chapter 2 安全、通知和标签 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 17
综述 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 17
警告、注意、重要事项以及注释的提示 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 18
仪器安全防护措施 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 19
活动部件或尖锐物. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 20
电气安全. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 20
激光安全 （适用于可选激光设备）. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 20
1 类激光小心标签 (1 级激光警告标签 ). . . . . . . . . . . . . . . . . . . . . . . . . . . . . 20
化学品注意事项 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 21
安全符号和标签 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 21
高压电击危险符号. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 21
注意安全符号. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 21
尖锐物标签. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 22
保险丝警告符号. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 22
致癌和生殖危害标签. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 22

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RoHS 通知 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 22
中国 RoHS 警告标签 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .23
其他仪器标签. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 23
环保标签 (WEEE) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .23
处理包含加汞组件的设备 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .24
CE 标志标签. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .24
RCM 标志标签 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .24
CSA 标识标签 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .24
Chapter 3 Sécurité, Consignes et étiquettes . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .25
Présentation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .25
Alertes « Avertissement, Mise en garde, Important et Remarque » . . . . . . . . . . .26
Précautions de sécurité pour l'instrument . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .27
Pièces mobiles ou Objets tranchants . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .28
Sécurité électrique . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .28
Sécurité laser (pour les appareils avec laser en option) . . . . . . . . . . . . . . . . . . .29
Étiquette de mise en garde : produit laser de classe 1 . . . . . . . . . . . . . . . . . . . .29
Précautions chimiques . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .30
Symboles et étiquettes de sécurité . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .30
Symbole « Haute tension, risque de choc électrique » . . . . . . . . . . . . . . . . . . .30
Symbole de sécurité Attention . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .31
Étiquette Objet tranchant . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .31
Symbole d'avertissement de fusible . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .31
Étiquette concernant les risques de cancer et de malformations congénitales. .31
Consignes RoHS . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .32
Étiquette de mise en garde RoHS (Chine) . . . . . . . . . . . . . . . . . . . . . . . . . . . . .32
Autres étiquettes de l'instrument . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .32
Étiquette de recyclage (WEEE) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .33
Élimination des appareils contenant des composants au mercure . . . . . . . . . . .33
Étiquette marque CE . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .33
Étiquette marque MRC . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .34
Étiquette marque CSA . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .34
Chapter 4 System Overview . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .35
Sample Handling System. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .38
Capillary Cartridge . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .40
Fluid Delivery, Power Supply, and Interlock . . . . . . . . . . . . . . . . . . . . . . . . . . . .41
Syringe Pump. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .41
High Voltage Power Supply . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .41
LED Indicators. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .41
Cartridge and Sample Cover Interlocks. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .41
UV Detector Optics . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .42
The Photo Diode Array (PDA) Detector (Optional) . . . . . . . . . . . . . . . . . . . . . . .43
The Laser Induced Fluorescence (LIF) Detector (Optional) . . . . . . . . . . . . . . . . .45
Chapter 5 System Procedures and Training Guide . . . . . . . . . . . . . . . . . . . . . . . .47
Controller and Instrument Start Up . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .47
Controller/Network Logon. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .47
License Key . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .47
P/ACE™ MDQ Plus Software . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .48
Enterprise Window . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .48
System Administration . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .49
Configuration . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .51
Online versus Offline. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .51

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How to Load the Cartridge and Samples . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 52
How to Run a Method. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 54
Analysis . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 57
Area Calculation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 60
Annotations . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 61
Peak Identity . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 62
Run Multiple Methods with a Sequence. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 62
Viewing Reports . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 66
Standard Reports . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 66
Method Custom Reports . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 66
Sequence Custom Report . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 66
Advanced Reports . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 66
Suitability Reports. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 67
Viewing and Printing Reports. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 68
Maintenance . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 68
Appendix A Specifications . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 69
P/ACE™ MDQ Plus System . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 69
Validated Controller Configuration . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 70
Sample Temperature Control . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 70
Capillary Temperature Control. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 70
Pressure and Vacuum System. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 71
Detector Specifications. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 71
UV Detector . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 71
Laser Induced Fluorescence (LIF) Detector (Optional) . . . . . . . . . . . . . . . . . . 72
Photo Diode Array (PDA) Detector (Optional) . . . . . . . . . . . . . . . . . . . . . . . . 72
Contact Us . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 75
Customer Training . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 75
Online Learning Center . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 75
SCIEX Support . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 75
CyberSecurity . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 75
Documentation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 76

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CHAPTER 1
Safety, Notices and Labels
Overview
A description of the symbols and labels that are used on the SCIEX P/ACE™ MDQ Plus Capillary
Electrophoresis System, or that are shown in this manual, can be found in this section.
Do not attempt to perform any procedure before carefully reading all instructions. If in doubt as to
how to proceed in any situation, contact your SCIEX representative.
SCIEX urges its customers and employees to comply with all national health and safety standards
such as the use of barrier protection. This may include, but is not limited to, protective eyewear,
gloves, and suitable laboratory attire when operating or maintaining this or any other automated
laboratory instrumentation.
WARNING
If the equipment is used in a manner not specified by SCIEX, the protection
provided by the equipment may be impaired.

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Alerts for Warning, Caution, Important, and Note
All Warnings and Cautions in this document include an exclamation point, framed within a triangle.
The exclamation point symbol is an international symbol which serves as a reminder that all safety
instructions should be read and understood before installation, use, maintenance, and servicing are
attempted.
WARNING
WARNING indicates a potentially hazardous situation which, if not avoided, could
result in death or serious injury. It may also be used to indicate the possibility of
erroneous data that could result in an incorrect diagnosis. May be used to indicate
the possibility of severe instrument damage.
CAUTION
CAUTION indicates a potentially hazardous situation, which, if not avoided, may
result in minor or moderate injury. It may also be used to alert against unsafe
practices. May be used to indicate the possibility of erroneous data that could
result in an incorrect diagnosis.
IMPORTANT Used for comments that add value to the step or procedure being performed. Following the
advice in the Important adds benefit to the performance of a piece of equipment or to a process.
NOTE Used to call attention to notable information that should be followed during installation, use, or
servicing of this equipment.

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Instrument Safety Precautions
WARNING
Risk of operator injury if:
• All doors, covers and panels are not closed and secured in place prior to and
during instrument operation.
• The integrity of safety interlocks and sensors is compromised.
• You contact moving parts.
• You mishandle broken parts.
• Doors, covers and panels are not opened, closed, removed and/or replaced
with care.
• Improper tools are used for troubleshooting.
To avoid injury:
• Keep doors, covers and panels closed and secured in place while the
instrument in use.
• Take full advantage of the safety features of the instrument. Do not defeat
safety interlocks and sensors.
• Acknowledge and act upon instrument alarms and error messages.
• Keep away from moving parts.
• Report any broken parts to your SCIEX representative.
• Use the proper tools when troubleshooting.
CAUTION
System integrity could be compromised and operational failures could occur if:
• This equipment is used in a manner other than specified. Operate the
instrument as instructed in the product manuals.
• You introduce software that is not authorized by SCIEX into your computer.
Only operate your system’s computer with software authorized by SCIEX.
• You install software that is not an original copyrighted version. Only use
software that is an original copyrighted version to prevent virus
contamination.
CAUTION
If you purchased this product from anyone other than SCIEX or an authorized
SCIEX distributor, and, if it is not presently under a SCIEX Service Maintenance
Agreement, SCIEX cannot guarantee that the product is fitted with the most
current mandatory engineering revisions or that you will receive the most current
information bulletins concerning the product. If you purchased this product from
a third party and would like further information concerning this topic, contact your

P/ACE™ MDQ Plus Capillary Electrophoresis System System Overview Guide
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SCIEX representative.
Moving Parts or Sharp Objects
WARNING
Risk of personal injury. To avoid injury due to moving parts, observe the following:
• Never attempt to exchange labware, reagents, or tools while the instrument
is operating.
• Never attempt to physically restrict any of the moving components of the
instrument.
• Keep the instrument work area clear to prevent obstruction of the movement.
Electrical Safety
To prevent electrically related injuries and property damage, properly inspect all electrical
equipment prior to use and immediately report any electrical deficiencies. Contact a SCIEX
representative for any servicing of equipment requiring the removal of covers or panels.
Laser Safety (for Optional Laser Device)
WARNING
This product may contain a laser module. The laser (optional) is designated as
“Class 3B.” The “3B” classification means that “direct intrabeam viewing of this
type of laser is always hazardous to personnel.”
The laser and several other integral components are contained in a sealed housing
that together comprise the laser assembly. The laser assembly has no user
serviceable parts. Service of the laser assembly is restricted to qualified SCIEX
Field Service Employees (FSE).
Therefore, the overall laser classification of the CE Instrument is “Class 1,”
defined as “lasers which are safe under reasonably foreseeable conditions of
operation.”
To prevent users from potentially harmful laser light, observe all safety warnings
and NEVER REMOVE THE OUTER CASING OF THE LASER ASSEMBLY.
IMPORTANT The laser markings noted above can vary depending on the type of device, and will be
described in the documentation provided with the module.