Sigma 2-16KC User manual

Refrigerated Centrifuge
2-16KC
Operating Manual

Operating Manual SIGMA 2-16KC, page 3 of 91
10/06
Thank you verymuch for your confidencein the productsof our company.
Our centrifuges are manufactured carefullyaccordingtothe highest qualitystandards and we
are sure that your demands will always be fulfilled.
Please read this manual carefullybefore installation ofthe centrifuge to ensure aproperand
safe operation.
Weare wishingyou a successful use of the centrifuge.
SIGMA LaborzentrifugenGmbH
P.O. Box1713 - 37507Osterode/Germany
Phone [phone] - Fax[phone]
Internet: www.sigma-zentrifugen.de
eMail: [e-mail]
SIGMA Service-Tel. [phone]

Operating Manual SIGMA 2-16KC, page 5 of 91
10/06
Konformit tserkl rung
(73/23/EWG; 89/336/EWG;98/37/EWG)
Statement of Conformity
(73/23/CEE;89/336/CEE; 98/37/CEE)
Déclaration de conformité
(73/23/CEE; 89/336/CEE; 98/37/CEE)
Die nachfolgendbezeichnete Maschine wurdein bereinstimmungmit denRichtlinien
73/23/EWG; 89/336/EWG und98/37/EWG hergestellt undgeprüft.
The followingmachine ismanufactured and testedin compliance with directions 73/23/CEE;
89/336/CEE and98/37/CEE.
La machine désignéeci-dessous est produit et examinéconforme auxdirectives 73/23/CEE;
89/336/CEE et 98/37/CEE
Bezeichnungder Maschine: Laborzentrifuge
Machine: LaboratoryCentrifuge
Désignation dela machine: Centrifugeusede laboratoire
Maschinentyp : 2 –16K
Type: 2 - 16 KC
Type de la machine: 2 - 16 KCH
Bestell Nr. : 10160, 10161, 10162, 10167, 10168, 10169, 10170
Part No.:
Réf. usine:
Normen: EN 61010-2-020
Standards: EN 61000-3-2 ; EN 61000-3-3
Normes: EN 61326
Sigma Laborzentrifugen
An der UnterenSöse 50
D-37520 Osterode
01.08.2002Geschäftsführer
ManagingDirector
Directeur Gérant
........................................................................
Fabr. Nr. Serial No. Num ro de fabrique
Konformitätserklärung dreisprachig 2-16k und 2-16KC 20020801.DOC

Operating Manual SIGMA 2-16KC, page 7 of 91
10/06
General Information
1.1 Technical data
1.2 Suitable accessories
1.3 Scope of supply
1.4 Standardsand regulations
1.5 Safetyinstructions
1.6 Symbol table
Descriptionof theCentrifuge
2.1 General outlay
2.2 Construction and constructive safetymeasures
2.3 Drive
2.4 Operation anddisplay
2.5 Electronic control
2.6 Safetydevices
2.6.1 Lid lock, cover closing device
2.6.2 Imbalancemonitoringsystem
2.6.3 Excess temperature in the centrifuge chamber
2.6.4 Rotor monitoring
2.6.5 Standstill monitoring
2.6.6 System check
2.6.7 Groundwire check
InstallationandStart-up
3.1 Unpackingof thecentrifuge
3.1.1 Transport safetydevice
3.2 Installation
3.2.1 Site
3.2.2 Connection/Fuse
3.2.3 Fuses / emergencycircuit breaker on site
3.3 Installation of rotorsand accessories
3.3.1 Fastening of angle rotorswith hermeticallysealedlid
3.4 Initial start-up
3.4.1 Switchingonof thecentrifuge
3.4.2 Opening lid
3.4.3 Installation of arotor

Operating Manual SIGMA 2-16KC, page 8 of 91
10/06
Operating Elements
4.1 Operating panel
4.1.1 Start-key
4.1.2 Stop-key
4.1.3 Lid-key
4.1.4 Knob
4.2 Display
4.2.1 Set
4.2.2 Speed
4.2.3 Relative CentrifugalForce (RCF)
4.2.4 Time
4.2.5 Temperature
4.2.6 Program
4.2.7 Rotor
4.2.8 Parameters
4.2.8.1 Acceleration
4.2.8.2 Deceleration
4.2.8.3 Radius
4.2.8.4 Density
4.2.8.5 Precooling
4.2.8.6 Start delay
4.2.9 Configuration
Operation
5.1 Selection, displayand alteration of program parameters
5.1.1 Selection and alteration oftheparameters and activation of the pre-
cooling, thestart delayand theautomaticlid openingafter endof run
5.1.2 Selection and alteration ofthe rotor part number
5.1.3 Alteration of the parameter values duringthe centrifuge run
5.1.4 Alteration of the configuration
5.1.4.1 Curves
5.1.4.2 Creation of curves for variable accelerationsand decelerations
5.1.4.3 Alteration of existing curves
5.1.5 Alteration of the contrast
5.1.6 Imbalancemonitoring
5.1.7 Shortrunand faststop
Programming
6.1 Load, save anddelete programs
6.1.1 Load a program
6.1.2 Save a program
6.1.3 Delete a program

Operating Manual SIGMA 2-16KC, page 9 of 91
10/06
Notes for Centrifugation
7.1 Practical notes for centrifugation
7.1.1 Practical notes for centrifugation for optional heating facilities
7.2 Forbidden centrifugingoperations
Care andMaintenance
8.1 Care and cleaningof centrifuge
8.2 Care and cleaningof accessories
8.3 Rotor pins
8.4 Glass breakage
8.5 Care and cleaningof thecondenser
8.6 Sterilization and disinfection of rotor chamber and accessories
8.6.1 Autoclaving
8.7 Checksbyoperator
Appendix
9.1 Slope ofthe specified curves, linear curves
9.2 Quadratic curves
9.3 Entrylimitations
9.4 Mathematical relations
9.4.1 Relative CentrifugalForce (RCF)
9.4.2 Density
9.5 Error correction
9.5.1 Centrifugecannot be started
9.5.2 Centrifugedecelerates during operation
9.5.3 Lid cannot be opened/closed
9.5.4 Emergencylid release
9.5.5 Problems with thecentrifuge?
9.6 Error codes
9.7 Speed-RCF-diagram
9.8 Declaration of decontamination/Return declaration
9.9 Form program data
9.10 Leaflet

Operating Manual SIGMA 2-16KC, page 11 of 91
10/06
1.1 Technical Data
Manufactuer: S I G MA
LaborzentrifugenGmbH
37520 Osterode, Germany
Type: 2-16KC
Electr. connection:
Protection class: see nameplate I
Power consumption (kVA):
Ratedpower (kW):
Max. current (A):
1
0,72
4 (230 V/50 Hz) respectively
8 (120 V/60 Hz)
Power data:
Max. speed (rpm):
Max. capacity(l):
Max. gravitational field (x g):
Max. kin. energy(Nm):
15 300
0,4
21 918
9 962
Further parameters
Time range:
Temperature setting range:
Temperature setting range
with optional heating facilities:
Programs:
Acceleration curves:
Deceleration curves:
Radius:
Rotor part no.:
9 h, 59 min/continuous operation
-20 to +40 °C
-20 to +60 °C
50 No. 1 - 50
10 linear No. 0 - 9
10 quadratic No.10 - 19
10 freelyprogrammable
No. 20 - 29
9 linear No. 1 - 9
1 brakeless No. 0
10 quadratic No.10 - 19
10 freelyprogrammable
No. 20 –29
max./min. s. 1.2
s. chapter 1.2
Dimensions:
Depth(mm):
Width (mm):
Height (mm):
Weight (kg):
EMC (acc. to EN 55011):
Noise level (dBA):
570
550
320
60
Class B
< 65
Notes ofuser:
Serial number:
Supplydate:
Inventorynumber:
Location:
Responsibility:
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The figures are validfor anambient temperature of23 °C+/- 2 °Candnominalvoltage +/- 5%.
The minimumtemperatures achievedduring run are<+4 °Canddependontype ofrotor,
speed and ambient temperature. For rotor 12132approx. +6 °Cwith14000 rpm.(Allowable
ambient temperature 10 °C - 35 °C; max. humidity80%.)
Optional heatingfacilities: Inorder to obtain the finaltemperature the rotor incl. buckets has to
be preheated at approx. 1500 rpm. Subject to technical alterations.

Operating Manual SIGMA 2-16KC, page 13 of 91
10/06
1.2 Accessories Suitablefor SIGMA2-16KC
Part No. Description Max. speed
(rpm)
Max.
gravitational
field (x g)
11124 Swing-out rotor 24x 1.5-2.2ml for
reactionvialse.g. 15008, 15040, incl.
hermetic aluminium lid, max. radius
7.4 cm, min. radius 3.5 cm 14 000 16 215
11409 Microhematocrite rotor for 24 capillary
tubes 1.5 x75 mm, 50 µl, incl. reader
17029, max. radius 9cm, min. radius
1.5 cm
12 000 14 489
15001 Microhematocrite capillarytubes,
heparinized, 1.5x 75mm, 50 µl, 200 pcs.
17005 Capillarysealing putty(6 plates)
17002 Reader for usewithmicrohematocrite
rotor
17029 Reader for 1capillarytube
17004 Magnifying glass
12139 Angle rotor 6 x27-30ml for sealedtubes
e.g. 15029, 15030, 15032, incl. hermetic
aluminium lid, max. radius 7.8 cm, min.
radius 2.2cm, angle 30
Attention!
Rotors from batchnumber 201/00maybe
run with lid, rotorsof older batches (e.g.
15/00 or ../99) have to be run without lid
15 300 20 414
12141 Angle rotor 10 x10-12ml for sealedtubes
e.g. 15000, 15010, 15039, incl. hermetic
aluminium lid, max. radius 7.6 cm, min.
radius 2.9cm, angle 35 15 300 19 890
12148 Angle rotor 24 x1.5-2.2ml for reaction
vials e.g. 15008, 15040, incl. hermetic
aluminium lid, max. radius 8.2 cm, min.
radius 5cm, angle45 15 300 21 460

Operating Manual SIGMA 2-16KC, page 14 of 91
10/06
Part No. Description Max. speed
(rpm)
Max.
gravitational
field (x g)
12132 Angle rotor 30 x1.5-2.2ml for reaction
vials e.g. 15008, 15040, incl. hermetic
aluminium lid, max. radius 10cm, min.
radius 6.7cm, angle 45 14 000 21 913
12104 Angle rotor incl. hermeticlid, aluminium,
for 12stripswith8PCR-tubes 0.2 ml each,
max. radius9.8 cm, min. radius 7.2cm,
angle 45 14 000 15 777/21475
12105 Angle rotor incl. hermeticlid, aluminium,
for 16stripswith5PCR-tubes 0.2 ml each,
max. radius9.6 cm, min. radius 7.3cm,
angle 45 14 000 15 996/21036
12072 Angle rotor 20 x15 ml incl. buckets13011,
for round or conical tubes, max. ∅17 x
120 mm, e.g. 15015, 15020, 15023, 15024,
Monovettes, culturetubes 15 ml 15115,
max. radius13.9 cm, min. radius8cm,
angle 33
3 900 2 364
12073 Angle rotor 30 x15 ml incl. buckets13011,
for round or conical tubes, max. ∅17 x
120 mm, e.g. 15015, 15020, 15023, 15024,
Monovettes, culturetubes 15 ml 15115,
2 lines, angle33
max. radius13.9 cm, min. radius8cm,
max. radius11.6 cm, min. radius 5.8 cm
3 900
3 900
2 364
1 973
12151 Angle rotor for 6 culture tubes50 ml
15151, incl. hermeticaluminium lid, max.
radius 9.5cm, min. radius 3 cm, angle28 9 000 8 603
13060 Adapter for 1 culturetube 15 ml 15115,
suitable for 12151, 13150, 19776
13079 Bottom adapter for 1tube 40-42ml 15051,
15052, 15054, suitable for 12151

Operating Manual SIGMA 2-16KC, page 15 of 91
10/06
Part No. Description Max. speed
(rpm)
Max.
gravitational
field (x g)
11190 Swing-out rotor 4x 100ml, complete,
consisting of rotor 11192, 4buckets
13097, 4glasstubes15100 and 4rubber
cushions 16051, max. radius 13.5 cm, min.
radius 5cm 5 000 3 773
11191 Swing-out rotor 16x 15ml, complete,
consisting of rotor 11192, 4multiple
carriers 13012, 16polystyrene tubes
15020 and 16rubber cushions 16015, max.
radius 13.4cm, min. radius 5.1 cm 5 000 3 745
11192 Swing-out rotor for 4 buckets or multiple
carriers 13004, 13009, 13012, 13022,
13040, 13041, 13047, 13097, 13150 5 000 3 773/3745
Buckets and multiplecarriers for 11192
13004 Multiple carrier, aluminium, incl. rubber
cushion, for 5tubes7 ml, max. ∅12.5/16
x 95 - 105mm, e.g. 15007, 15027
13009 Multiple carrier, aluminium, for 5tubes
5 ml, max. ∅12.5/16.5 x 65 - 85mm, flat
and round bottom tubes, e.g. 15060,
Vacutainer tubes
13012 Multiple carrier, aluminium, incl. rubber
cushion 16015, for 4 tubes 5-15ml, max.
∅17x 90 - 110 mm, e.g. 15015, 15020,
15023, 15024, Monovettes
13022 Bucket, aluminium, incl. rubber cushion
16025, for 1glass tube 25-30 ml, max.
∅24.5 x95 - 110mm, e.g. 15025, 15026,
15033
13047 Bucket, aluminium, incl. rubber cushion
16051, for 1tube 50ml, max. ∅35.5 x
95 - 110 mm, e.g. 15049, 15050, 15056

Operating Manual SIGMA 2-16KC, page 16 of 91
10/06
Part No. Description Max. speed
(rpm)
Max.
gravitational
field (x g)
13097 Bucket, aluminium, incl. rubber cushion
16051, for 1tube 100ml, max. ∅45.5 x
95 - 105 mm, e.g. 15100, 15102, 15103,
15106
13041 Bucket, aluminium, incl. screwcap17130,
for round carriers 14029, 14030, 14031,
14032, 14033, 14034, 14035, max. length of
tubes 110mm
14029 Round carrier for 5 tubes 5-7ml, max. ∅
12.5/15x 65 - 105 mm, e.g. 15007, 15027,
15060, polypropylene, suitable for 13041
14033 Round carrier for 4 tubes 5 ml, ∅13.5/17.5 x
70 - 110 mm, e.g. Vacutainer tubes,
polypropylene, suitable for 13041
14034 Round carrier for 3 tubes 10-15ml, max.
∅17.3/19x 80 - 110 mm, e.g. 15020, 15023
and Monovettes9 and10 ml, polyethylene,
suitable for 13041
14030 Round carrier for 4 tubes 10-12ml, max.
∅16.2/17.5 x80 - 110mm, e.g. 15000,
15010, 15015, 15024, 15039, polyethylene,
suitable for 13041
14031 Round carrier for 1 glass tube25 ml, max.
∅25/30 x70 - 105mm, e.g. 15025, 15026,
15033, polypropylene, suitable for 13041
14035 Round carrier for 1 sterilin tube 30ml,
graduated upto20 ml, with skirt, incl. cap,
max. ∅25/31x 65 - 95 mm, polypropylene,
suitable for 13041,
see www.bibby-sterilin.co.uk, no. 03008
14032 Round carrier for 1 tube 50 ml, max. ∅
35/38 x70 - 105mm, e.g. 15049, 15050,
15056, polypropylene, suitable for 13041
17130 Sealing cap, polysulfone, clear, for 13041

Operating Manual SIGMA 2-16KC, page 17 of 91
10/06
Part No. Description Max. speed
(rpm)
Max.
gravitational
field (x g)
13150 Bucket, aluminium, incl. screwcap17151,
for 1culturetube50 ml 15151
17151 Sealing cap, polysulfone, clear, for 13150
13060 Adapter for 1 culturetube 15 ml 15115,
suitable for 12151, 13150, 19776
13040 Bucket, aluminium, incl. screwcap17140,
for round carriers 17205, 17215, 17225,
17250, max. length of tubes 110 mm
17140 Sealing cap, polysulfone, clear, for 13040
17205 Round carrier for 4 tubes, max. ∅13.5/16.5
x 70 - 90mm, e.g. Vacutainer tubes,
polypropylene, suitable for 13040
17215 Round carrier for 3 tubes 10-15ml, max.
∅17x 90 - 100 mm, e.g. 15015, 15020,
15023, 15024, polypropylene, suitablefor
13040
17225 Round carrier for 1 tube 25-30ml, max.
∅25.4/28x 70 - 105 mm, e.g. 15025, 15026,
15029, 15030, 15032, 15033, polypropylene,
suitable for 13040
17222 Round carrier for 1 sterilin tube 30ml,
graduated upto20 ml, with skirt, incl. cap,
max. ∅25/31x 65 - 95 mm, polypropylene,
suitable for 13040,
see www.bibby-sterilin.co.uk, no. 03008
17250 Round carrier for 1 tube 50 ml, max. ∅
35/38 x70 - 105mm, e.g. 15049, 15050,
15056, polyethylene, suitable for 13040

Operating Manual SIGMA 2-16KC, page 18 of 91
10/06
Part No. Description Max. speed
(rpm)
Max.
gravitational
field (x g)
11122 Swing-out rotor for microtiter plates.
incl. 2carriers13222,
radius edge12.3 cm,
radius max. 10.5 cm,
radius min. 6.5cm,
max. plate height 56 mm
3 000 1 238
1 057
654
11123 Swing-out rotor for microtiter plates,
incl. 2carriers13223,
radius edge11.9 cm,
radius max. 10cm,
radius min. 6.5cm,
max. plate height 48 mm
4 000 2 129
1 789
1 163
Adaptors and PlasticVessels
13000 Adapter for reaction vials 0.25-0.4 ml 15014,
suitable for 11124, 12132, 12148,
polyallomer
13002 Adapter for reaction vials 0.5-0.75 ml 15005,
Ø7.9/10x 28/31mm, suitable for 11124,
12132, 12148, polyallomer
13021 Adapter for PCR-tube 0.2 ml, ∅5.85/6.95 x
20/23.4mm, suitablefor 11124, 12132,
12148, polyallomer
13059 Adapter for 1 tube withscrewcap10-12ml,
max. Ø16.2/19x75 - 85 mm, e.g. 15000,
15010, 15039
15014 Reaction vials0.4 ml (Beckman system),
polypropylene, 1 packcontains100 pcs.,
suitable for 13000
15005 Reaction vials0.5 ml, Ø7.9/10x 28/31mm,
1 pack contains100 pcs., suitable for 13002
15008 Reaction vials1.5 ml, 1pack contains
100 pcs., suitable for 11124, 12132, 12148
15040 Reaction vials2.2 ml, 1pack contains
100 pcs., suitable for 11124, 12132, 12148

Operating Manual SIGMA 2-16KC, page 19 of 91
10/06
Part No. Description
15010 Polycarbonate tube with screwcap12 ml,
∅16.1 x81.1mm, suitable for 12141, 13059
15000 ditto, Teflon
15039 ditto, polypropylene
13026 Stainlesssteel tube10 ml, ∅15.7 x76 mm,
closeablewithcap 17126, suitable for
12141, 13059
17126 Stainlesssteel sealing cap for 13026
15020 Polystyrene tube 15 ml, ∅17x 100mm,
suitable for 11191, 12072, 12073, 13012,
14034, 17215
15021 Polypropylene stopper for 15020, 15023
15023 Polypropylene tube 15 ml, ∅17x 100mm,
suitable for 12072, 12073, 13012, 14034,
17215
15115 Culturetubeincl. screwcap15 ml, suitable
for 12072, 12073, 13060
15029 Teflon tubewithscrewcap 28 ml, Ø25.3x
96 mm, 12139, 17225
15030 Polycarbonate tube with screwcap30 ml,
∅25.3 x92 mm, suitable for 12139, 17225
15032 Polypropylene tube with screwcap28 ml,
∅25.3 x92 mm, suitable for 12139, 17225
13079 Bottom adapter for 1tube 42-50ml 15051,
15052, 15054, suitable for 12151
15049 Polycarbonate tube50 ml, graduated
0 - 50 ml instepsof 1 ml, ∅34x 100mm,
suitable for 13047
15051 Teflon tubewithscrewcap 42 ml, ∅28.5 x
107 mm, suitablefor 13079

Operating Manual SIGMA 2-16KC, page 20 of 91
10/06
Part No. Description
15052 Polypropylene tube with screwcap42 ml,
∅28.8 x107 mm, suitable for 13079
15054 Polycarbonate tube with screwcap40 ml,
∅28.8 x107 mm, suitable for 13079
15151 Culturetubewithscrewcap50 ml, pointed
bottom, suitablefor 12151, 13150
15102 Polypropylene tube 100 ml, ∅45 x100 mm,
suitable for 13097
15103 Polycarbonate tube100 ml, ∅45 x100 mm,
graduated 2 - 100ml in steps of 2ml,
suitable for 13097
Centrifuge GlassTubes
15007 Centrifuge glass tube 7ml, ∅12 x100 mm,
suitable for 13004, 14029
15027 ditto, graduated, 0 - 5.5ml in steps of 0.1 ml
15015 Centrifuge glass tube 15 ml, ∅16 x100
mm, suitable for 12072, 12073, 13012,
14030, 17215
15024 ditto, graduated, 0 - 10ml instepsof 0.1ml
15025 Centrifuge glass tube 25 ml, ∅24 x100
mm, suitable for 13022, 14031, 17225
15026 ditto, graduated, 5 - 25ml instepsof 1 ml
15050 Centrifuge glass tube 50 ml, ∅34 x100
mm, suitable for 13047, 14032, 17250
15056 ditto, graduated, 4 - 50ml instepsof 1 ml
15100 Centrifuge glass tube 100 ml, ∅44 x
100 mm, suitablefor 11190, 13097
15106 ditto, graduated, 1 - 100 ml in steps of 1ml
Further accessories available onrequest.