Sigma 2-16KHL User manual

Translation of the original operating manual
sb
02/2013
Rev. 1.19 of 25/04/2019
Sigma 2-16KHL; ;
part no. 0700802
Sigma 2-16KHL
from serial no. 147260
Refrigerated Centrifuge
with Heating System
Operating Manual
Please retain for later use!

Refrigerated Centrifuge with Heating System Sigma 2-16KHL
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Translation of the original operating manual, part no. 0700802

Refrigerated Centrifuge with Heating System Sigma 2-16KHL
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Translation of the original operating manual, part no. 0700802
In case of inquiries, please state the following numbers:
Order number:
Serial number:
© Copyright by
Sigma Laborzentrifugen GmbH
An der Unteren Söse 50
37520 Osterode am Harz
Germany
Tel.: [phone] / 5007-0
Fax: [phone] / 5007-12
Web: www.sigma-zentrifugen.de
E-mail: [e-mail]

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Translation of the original operating manual, part no. 0700802

Refrigerated Centrifuge with Heating System Sigma 2-16KHL
Table of contents
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Translation of the original operating manual, part no. 0700802
1General information.............................................................................................................9
1.1 Importance of the operating manual..................................................................................9
1.2 Intended use.....................................................................................................................9
1.3 Warranty and liability.........................................................................................................9
1.4 Copyright........................................................................................................................10
1.5 Standards and regulations..............................................................................................10
1.6 Scope of supply..............................................................................................................10
2Layout and mode of operation.......................................................................................... 11
2.1 Layout of the centrifuge ..................................................................................................11
2.1.1 Functional and operating elements............................................................................11
2.1.2 Name plate................................................................................................................12
2.2 Mode of operation...........................................................................................................13
2.2.1 Centrifugation principle..............................................................................................13
2.2.2 Area of application.....................................................................................................13
2.2.2.1 Speed, radius, and relative centrifugal force ......................................................14
2.2.2.2 Density...............................................................................................................14
3Safety..................................................................................................................................15
3.1 Marking of the unit..........................................................................................................15
3.2 Explanation of the symbols and notes.............................................................................16
3.3 Responsibility of the operator..........................................................................................17
3.4 Operating personnel .......................................................................................................17
3.5 Informal safety instructions .............................................................................................17
3.6 Safety instructions ..........................................................................................................18
3.6.1 Electrical safety .........................................................................................................18
3.6.2 Mechanical safety......................................................................................................18
3.6.3 Fire prevention...........................................................................................................19
3.6.4 Chemical and biological safety ..................................................................................19
3.6.5 Safety instructions for centrifugation..........................................................................20
3.6.6 Resistance of plastics................................................................................................20
3.6.7 Safety of rotors and accessories................................................................................21
3.6.7.1 Service life.........................................................................................................21
3.7 Safety devices ................................................................................................................22
3.7.1 Lid lock device...........................................................................................................22
3.7.2 Standstill monitoring system......................................................................................22
3.7.3 System check............................................................................................................22
3.7.4 Earth conductor check...............................................................................................22
3.7.5 Imbalance monitoring system ....................................................................................22
3.7.6 Temperature monitoring system ................................................................................22
3.7.7 Rotor monitoring system............................................................................................22
3.8 Measures in the event of hazards and accidents ............................................................23
3.9 Remaining hazards.........................................................................................................23

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4Storage and transport ....................................................................................................... 24
4.1 Dimensions and weight...................................................................................................24
4.2 Storage conditions..........................................................................................................24
4.3 Notes on transport..........................................................................................................24
4.4 Packaging.......................................................................................................................25
4.5 Transport safety device...................................................................................................25
5Set-up and connection ......................................................................................................26
5.1Installation site................................................................................................................26
5.2 Power supply..................................................................................................................26
5.2.1 Type of connection ....................................................................................................26
5.2.2 Customer-provided fuses...........................................................................................27
6Using the centrifuge.......................................................................................................... 28
6.1 Initial start-up..................................................................................................................28
6.2 Switching the centrifuge on.............................................................................................28
6.2.1 Opening and closing the lid........................................................................................28
6.2.2 Installation of rotors and accessories.........................................................................28
6.2.2.1 Installation of a rotor ..........................................................................................28
6.2.2.2 Installation of an angle rotor with a hermetic lid..................................................29
6.2.2.3 Installation of the microhaematocrit rotor............................................................30
6.2.2.4 Installation of accessories..................................................................................31
6.2.2.5 Adapters ............................................................................................................33
6.2.2.6 Vessels..............................................................................................................33
6.3 Control system "Spincontrol L"........................................................................................34
6.3.1 User interface............................................................................................................34
6.3.2 Manual mode.............................................................................................................35
6.3.2.1 Starting a centrifugation run...............................................................................35
6.3.2.2 Interrupting a centrifugation run .........................................................................35
6.3.2.3 Interrupting a deceleration process....................................................................35
6.3.2.4 Selection, display, and modification of data........................................................35
6.3.2.1 Speed / relative centrifugal force (RCF) .............................................................36
6.3.2.2 Runtime .............................................................................................................36
6.3.2.3 Temperature ("Temp") .......................................................................................38
6.3.2.4 Rotor selection...................................................................................................40
6.3.2.5 Acceleration and deceleration curves.................................................................41
6.3.2.6 Automatic lid opening function ("AutoOpen")......................................................41
6.3.2.7 Sound signal ("Buzzer").....................................................................................42
6.3.2.8 Program lock ("ProgLock").................................................................................42
6.3.2.9 Cycle display ("Cycles").....................................................................................43
6.3.2.10 Input lock ...........................................................................................................44
6.3.3 Program mode...........................................................................................................44
6.3.3.1 Saving a program...............................................................................................45
6.3.3.2 Loading a program.............................................................................................45
6.4 Switching the centrifuge off.............................................................................................46

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7Malfunctions and error correction....................................................................................47
7.1 General malfunctions......................................................................................................47
7.1.1 Emergency lid release...............................................................................................48
7.2 Table of error codes........................................................................................................49
7.3 Service contact...............................................................................................................50
8Maintenance and service................................................................................................... 51
8.1 Maintenance...................................................................................................................51
8.1.1 Centrifuge..................................................................................................................51
8.1.1.1 Condenser (only refrigerated centrifuges with an air-cooled refrigeration
system)..............................................................................................................52
8.1.2 Accessories...............................................................................................................52
8.1.2.1 Plastic accessories ............................................................................................53
8.1.3 Rotors, buckets and carriers......................................................................................53
8.1.4 Microhaematocrit rotor...............................................................................................53
8.1.5 Load-bearing bolts.....................................................................................................54
8.1.6 Glass breakage .........................................................................................................55
8.2 Sterilisation and disinfection of the rotor chamber and accessories ................................55
8.2.1 Autoclaving................................................................................................................56
8.3 Service ...........................................................................................................................57
8.4 Return of defective centrifuges or parts ..........................................................................58
9Disposal.............................................................................................................................. 60
9.1 Disposal of the centrifuge ...............................................................................................60
9.2 Disposal of the packaging...............................................................................................60
10 Technical data.................................................................................................................... 61
10.1 Ambient conditions .........................................................................................................62
10.2 Technical documentation................................................................................................62
11 Appendix ............................................................................................................................ 63
11.1 Range of accessories .....................................................................................................63
11.1.1 Rotor radii..................................................................................................................63
11.2 Speed-gravitational-field-diagram ...................................................................................65
11.3 Acceleration and deceleration curves .............................................................................66
11.4 Table of the service life of rotors and accessories...........................................................67
11.5 Resistance data..............................................................................................................68
11.6 EC declaration of conformity...........................................................................................73
11.7 Declaration of conformity –China RoHS 2......................................................................75
12 Index...................................................................................................................................77

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1 General information
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Translation of the original operating manual, part no. 0700802
1 General information
1.1 Importance of the operating manual
A fundamental requirement for the safe and trouble-free operation of the
centrifuge is to be familiar with the fundamental safety instructions and all
possible hazards.
The operating manual includes important information concerning the safe
operation of the centrifuge.
This operating manual and, in particular, the notes on safety and hazards
must be observed by all persons operating the centrifuge.
In addition, the local rules and regulations for the prevention of accidents
must be complied with.

1.2 Intended use
Centrifuges are power-driven machines that separate liquids from solid
matter, liquid mixtures, or solid mixtures by centrifugal force. They are
solely intended for this purpose. Any other use beyond this area of
application is regarded as improper use. Sigma Laborzentrifugen GmbH
cannot be held liable for any damage resulting from such improper use.
The intended use also includes
•observation of all the notes and instructions included in the operating
manual and
•compliance with the care, cleaning, and maintenance instructions.

1.3 Warranty and liability
The warranty and liability are subject to our "General Conditions" that were
distributed to the operator upon the conclusion of the contract.
Warranty and liability claims are excluded if they are due to:
•improper use.
•non-compliance with the safety instructions and hazard warnings in the
operating manual.
•improper installation, start-up, operation, or maintenance of the
centrifuge.

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1.4 Copyright The copyright concerning the operating manual remains with
Sigma Laborzentrifugen GmbH.
The operating manual is solely intended for the operator and their
personnel. It includes instructions and information that must not be
•duplicated,
•distributed, or
•communicated in any other way.
Non-compliance may be prosecuted under criminal law.

1.5 Standards and regulations
EC declaration of conformity
(see chapter 11.6 - "EC declaration of conformity")
1.6 Scope of supply
The centrifuge comprises:
•1 power cord with an IEC C13 connector
•1 rotor wrench
Part no. 930 100
•1 tube (30 g) heavy-duty grease for load-
bearing bolts
Part no. 71 401
Documentation
Operating manual incl. EC declaration of conformity
(see chapter 11.6 - "EC declaration of conformity")
Accessories
According to your order, our order confirmation, and your delivery note.