Optika Italy B-1000 Series User manual

Model
B-1000
B-1000BF
B-1000PH
B-1000TI-2
B-1000TI-3
B-1000TI-5
B-1000TI-10
B-1000 Series
INSTRUCTION MANUAL
Ver. 2.1 2019

Page 2
1. Warning 3
2. Symbols and conventions 3
3. Safety Information 3
4. Intended use 3
5. Overview 4
5.1 Manual version 4
5.2 Motorised version 6
6. Unpacking 8
7. Assembling 8
7.1 Manual version 8
7.2 Motorised version 9
7.3 Assembling the microscope 10
7.4 Only for motorised version 12
8. Summaryofbrighteldobservationprocedures 13
9. Use of the microscope 14
9.1 General switch on 14
9.2 Control keyboard 14
9.3 Brightness adjustment 14
9.4 Adjust the observation head 15
9.5 Adjust the interpupillary distance 15
9.6 Diopter adjustment 15
9.7 Use of eyeshields 16
9.8 Focus tension adjustment 16
9.9 Focus stop lever 16
9.10 Stage 17
9.11 Condenser centering 17
9.12 Use of oil immersion objective 18
9.13 Use of ALC system 19
9.14 Nosepiece rotation 19
9.15 Focusing 19
9.16 Stage 19
10. Useofuniversalcondenserforbrighteld/darkeld/phasecontrast 20
10.1 Brighteldobservation(BF) 20
10.2 Darkeldobservation(DF) 20
10.3 Phasecontrastobservation(PH) 21
10.4 Useofgreenlter 22
11. DIC observation 23
11.1 Koehler DIC transmitted light 23
1.2 Nomarski DIC transmitted light 24
12. Microphotography 26
12.1 Installing the “C” mount adapter 26
12.2 Useofreexcamera 26
13. Maintenance 27
14. Troubleshooting 28
Equipment disposal 30
Summary

Page 3
1. Warning
This microscope is a scientic precision instrument designed to last for many years with a minimum of
maintenance. It is built to high optical and mechanical standards and to withstand daily use. We remind you
that this manual contains important information on safety and maintenance, and that it must therefore be made
accessible to the instrument users. We decline any responsibility deriving from incorrect instrument use uses
that does not comply with this manual.
2. Symbols and conventions
The following chart is an illustrated glossary of the symbols that are used in this manual.
CAUTION
This symbol indicates a potential risk and alerts you to proceed with caution.
ELECTRICALSHOCK
This symbol indicates a risk of electrical shock.
3. Safety Information
Avoiding Electrical Shock
Before plugging in the power supply, make sure that the supplying voltage of your region matches with the
operation voltage of the equipment and that the lamp switch is in off position. Users should observe all safety
regulations of the region. The equipment has acquired the CE safety label. However, users have full responsibility
to use this equipment safely. Please follow the guidelines below, and read this manual in its entirety to ensure
safe operation of the unit.
4. Intended use
Standard models
For research and teaching use only. Not intended for any animal or human therapeutic or diagnostic use.
IVD Models
Also for diagnostic use, aimed at obtaining information on the physiological or pathological situation of the
subject.

Page 4
5. Overview
5.1 Manual version EYEPIECE
PHOTO / TV
PORT
OBSERVATION
HEAD
ALC SYSTEM
DIOPTER
ADJUSTMENT
RING
DIC PRISM
SLOT
CONTROL
KEYBOARD
MAIN
SWITCH
FOCUS LOCK
LEVER INTENSITY
ADJUSTMENT
DIAL
FIELD
DIAPHRAGM
CONDENSER
STAGE
CONDENSER
HEIGHT
ADJUSTMENT
KNOB
NOSEPIECE
OBJECTIVES

Page 5
FILTER
HOLDER
COARSE
FOCUS KNOB
FINE FOCUS
KNOB
TENSION
ADJUSTMENT
COLLAR
APERTURE
DIAPHRAGM
LIGHT PATH
SELECTOR
LEVER
STAGE
SLIDE
HOLDER
X-Y STAGE
MOVEMENT
KNOBS
CONDENSER
CENTERING
SCREWS
ALC SOCKET

Page 6
5.2 Motorised version
Only the parts related to the motor are highlighted; all the other components of the microscope remain unchan-
ged compared to the manual version.
NOSEPIECE
FOCUS
MOTOR
X-AXIS MOVEMENT
KNOB (MANUAL
MOVEMENT)
STAGE

Page 7
STAGE
CONNECTION
CABLE
NOSEPIECE
ROTATION
BUTTONS
X-AXIS MOVEMENT
KNOB (MANUAL
MOVEMENT)

Page 8
6. Unpacking
The microscope is housed in a moulded Styrofoam container. Remove the tape from the edge
of the container and lift the top half of the container. Take some care to avoid that the opti-
cal items (objectives and eyepieces) fall out and get damaged. Using both hands (one around the
arm and one around the base), lift the microscope from the container and put it on a stable desk.
Do not touch with bare hands optical surfaces such as lenses, lters or glasses. Traces of grease or
other residuals may deteriorate the nal image quality and corrode the optics surface in a short time.
7. Assembling
7.1 Manual version
Once opened the box, the microscope parts (manual version) are the following:
① Frame
② Objectives
③ Stage
④ Condenser
⑤ Observation head
⑥ Eyepieces
⑦ ALC system (M-1030) ( Optional)
⑧ Power supply
③
④
⑤
⑥
⑦
⑧
①②

Page 9
⑪⑩
⑨
7.2 Motorised version
Once opened the box, the microscope parts (motorised version) are the following:
① Frame
② Objectives
③ Stage
④ Condenser
⑤ Observation head
⑥ Eyepieces
⑦ ALC system (M-1030) ( Optional)
⑧ Microscope power supply
⑨ Motorised parts power supply
⑩ Serial cable
⑪ PS/2 mouse
①②
③
④
⑤
⑥
⑦
⑧
⑧

Page 10
7.3 Assembling the microscope
1. Put the microscope stand on a solid ta-
ble. Insert M-1030 attachment (if provi-
ded) above the stand, using the 2mm Al-
len wrench to tighten the screw. (Fig.1)
2. Connect the cable of the ALC (Automa-
tic Light Control) system to the socket pla-
ced on the right side of the frame. (Fig.2)
3. Insert the optical head above the attachment, using
the 2mm Allen wrench to tighten the screw. (Fig.3)
4. Insert eyepieces into the empty eyepiece slee-
ves. (Fig. 4)
Fig. 1
Fig. 2
Fig. 3
Fig. 4