Optika Italy B-1000 Series User manual

Model
B-1000
B-1000BF
B-1000PH
B-1000TI-2
B-1000TI-3
B-1000TI-5
B-1000TI-10
B-1000 Series
INSTRUCTION MANUAL
Ver. 2.1 2019

Page 2
1. Warning 3
2. Symbols and conventions 3
3. Safety Information 3
4. Intended use 3
5. Overview 4
5.1 Manual version 4
5.2 Motorised version 6
6. Unpacking 8
7. Assembling 8
7.1 Manual version 8
7.2 Motorised version 9
7.3 Assembling the microscope 10
7.4 Only for motorised version 12
8. Summaryofbrighteldobservationprocedures 13
9. Use of the microscope 14
9.1 General switch on 14
9.2 Control keyboard 14
9.3 Brightness adjustment 14
9.4 Adjust the observation head 15
9.5 Adjust the interpupillary distance 15
9.6 Diopter adjustment 15
9.7 Use of eyeshields 16
9.8 Focus tension adjustment 16
9.9 Focus stop lever 16
9.10 Stage 17
9.11 Condenser centering 17
9.12 Use of oil immersion objective 18
9.13 Use of ALC system 19
9.14 Nosepiece rotation 19
9.15 Focusing 19
9.16 Stage 19
10. Useofuniversalcondenserforbrighteld/darkeld/phasecontrast 20
10.1 Brighteldobservation(BF) 20
10.2 Darkeldobservation(DF) 20
10.3 Phasecontrastobservation(PH) 21
10.4 Useofgreenlter 22
11. DIC observation 23
11.1 Koehler DIC transmitted light 23
1.2 Nomarski DIC transmitted light 24
12. Microphotography 26
12.1 Installing the “C” mount adapter 26
12.2 Useofreexcamera 26
13. Maintenance 27
14. Troubleshooting 28
Equipment disposal 30
Summary

Page 3
1. Warning
This microscope is a scientic precision instrument designed to last for many years with a minimum of
maintenance. It is built to high optical and mechanical standards and to withstand daily use. We remind you
that this manual contains important information on safety and maintenance, and that it must therefore be made
accessible to the instrument users. We decline any responsibility deriving from incorrect instrument use uses
that does not comply with this manual.
2. Symbols and conventions
The following chart is an illustrated glossary of the symbols that are used in this manual.
CAUTION
This symbol indicates a potential risk and alerts you to proceed with caution.
ELECTRICALSHOCK
This symbol indicates a risk of electrical shock.
3. Safety Information
Avoiding Electrical Shock
Before plugging in the power supply, make sure that the supplying voltage of your region matches with the
operation voltage of the equipment and that the lamp switch is in off position. Users should observe all safety
regulations of the region. The equipment has acquired the CE safety label. However, users have full responsibility
to use this equipment safely. Please follow the guidelines below, and read this manual in its entirety to ensure
safe operation of the unit.
4. Intended use
Standard models
For research and teaching use only. Not intended for any animal or human therapeutic or diagnostic use.
IVD Models
Also for diagnostic use, aimed at obtaining information on the physiological or pathological situation of the
subject.

Page 4
5. Overview
5.1 Manual version EYEPIECE
PHOTO / TV
PORT
OBSERVATION
HEAD
ALC SYSTEM
DIOPTER
ADJUSTMENT
RING
DIC PRISM
SLOT
CONTROL
KEYBOARD
MAIN
SWITCH
FOCUS LOCK
LEVER INTENSITY
ADJUSTMENT
DIAL
FIELD
DIAPHRAGM
CONDENSER
STAGE
CONDENSER
HEIGHT
ADJUSTMENT
KNOB
NOSEPIECE
OBJECTIVES

Page 5
FILTER
HOLDER
COARSE
FOCUS KNOB
FINE FOCUS
KNOB
TENSION
ADJUSTMENT
COLLAR
APERTURE
DIAPHRAGM
LIGHT PATH
SELECTOR
LEVER
STAGE
SLIDE
HOLDER
X-Y STAGE
MOVEMENT
KNOBS
CONDENSER
CENTERING
SCREWS
ALC SOCKET

Page 6
5.2 Motorised version
Only the parts related to the motor are highlighted; all the other components of the microscope remain unchan-
ged compared to the manual version.
NOSEPIECE
FOCUS
MOTOR
X-AXIS MOVEMENT
KNOB (MANUAL
MOVEMENT)
STAGE

Page 7
STAGE
CONNECTION
CABLE
NOSEPIECE
ROTATION
BUTTONS
X-AXIS MOVEMENT
KNOB (MANUAL
MOVEMENT)

Page 8
6. Unpacking
The microscope is housed in a moulded Styrofoam container. Remove the tape from the edge
of the container and lift the top half of the container. Take some care to avoid that the opti-
cal items (objectives and eyepieces) fall out and get damaged. Using both hands (one around the
arm and one around the base), lift the microscope from the container and put it on a stable desk.
Do not touch with bare hands optical surfaces such as lenses, lters or glasses. Traces of grease or
other residuals may deteriorate the nal image quality and corrode the optics surface in a short time.
7. Assembling
7.1 Manual version
Once opened the box, the microscope parts (manual version) are the following:
① Frame
② Objectives
③ Stage
④ Condenser
⑤ Observation head
⑥ Eyepieces
⑦ ALC system (M-1030) ( Optional)
⑧ Power supply
③
④
⑤
⑥
⑦
⑧
①②

Page 9
⑪⑩
⑨
7.2 Motorised version
Once opened the box, the microscope parts (motorised version) are the following:
① Frame
② Objectives
③ Stage
④ Condenser
⑤ Observation head
⑥ Eyepieces
⑦ ALC system (M-1030) ( Optional)
⑧ Microscope power supply
⑨ Motorised parts power supply
⑩ Serial cable
⑪ PS/2 mouse
①②
③
④
⑤
⑥
⑦
⑧
⑧

Page 10
7.3 Assembling the microscope
1. Put the microscope stand on a solid ta-
ble. Insert M-1030 attachment (if provi-
ded) above the stand, using the 2mm Al-
len wrench to tighten the screw. (Fig.1)
2. Connect the cable of the ALC (Automa-
tic Light Control) system to the socket pla-
ced on the right side of the frame. (Fig.2)
3. Insert the optical head above the attachment, using
the 2mm Allen wrench to tighten the screw. (Fig.3)
4. Insert eyepieces into the empty eyepiece slee-
ves. (Fig. 4)
Fig. 1
Fig. 2
Fig. 3
Fig. 4

Page 11
5. Insert the condenser under the stage: position
until it is well inserted into its holder (under the
condenser there is a pin that must fully enter the
guide of the holder). (Fig. 5)
6. Lock the condenser xing knob ①.
7. Mount the stage: lower the support using
the coarse focus knob, then place the stage
and rmly tighten the lock screw ②. (Fig. 6)
8. Screw the objectives into the revolving no-
sepiece, in order of magnication. (Fig. 7)
9. Insert the power supply jack in the socket placed
in the rear side of the frame. (Fig. 8)
Fig. 5
①
Fig. 6
②
Fig. 7
Fig. 8

Page 12
10. Assemble the stage in the same way as the
manual version. Check the perfect alignment of
the rear part of the stage with the rear arm of
the frame. An imperfect alignment could lead to
an incorrect functioning of the system. (Fig. 9)
11. Connect the cable ① from the stage to the frame and
tight the locking screws of the connectors ②. (Fig. 10)
12. Connect the provided cables: ③ 12V power sup-
ply for the motorised parts; ④6V microscope
power supply; ⑤ serial cable; ⑥ PS/2 mouse.
Connect power cables as the last step. (Fig. 11)
7.4 Only for motorised version
Fig. 9
Fig. 10
②
①
Fig. 11
③ ④ ⑤⑥

Page 13
8. Summaryofbrighteldobservationprocedures
Main switch
(Used commands) (Chapter)
Slide holder
Revolving nosepiece
Observation head
Diopter adjustment ring
Condenser centering
Aperture diaphragm
Field diaphragm
Revolving nosepiece
Light adjustment dial
Coarse and fine focusing knobs
Coarse and fine focusing knobs
Bring on “1” the main switch
Put a slide on the stage
Insert 10X objective into the light path
Focus the specimen
Begin observation
Adjust field and aperture iris diaphragms
Insert desired objective for the observation and focus the
specimen
Adjust interpupillary distance
Adjust diopters
Center optical axis
Adjust light intensity
9.1
9.9
5
5
5
5
9.5
9.6
9.11
9.11
9.11
9.3
ON-OFF key on the keyboard
Light adjustment dial
Turn on the microscope and adjust light intensity.9.2
9.3
ALC system 9.13

Page 14
9. Use of the microscope
9.1 General switch on
To activate the transmitted light illuminator, insert the
plug of the external power supply into the mains so-
cket and turn the main switch ①, located on the left
side of the stand, to the position “1”. (Fig. 12)
9.2 Control keyboard
B-1000 illumination can be managed through the
keyboard placed on the left of the stand. (Fig. 13)
• ON-OFF (②): press this key (after switching the
main switch on “1”) to turn ON or OFF the micro-
scope LED.
• BOOST (③): press this button in order to in-
cre-ase the brightness (useful for high-magni-
cation objectives or very opaque specimens).
Do not enable boost mode while observing
with low magnication objectives (4x, 10x)
with fully open diaphragm: the high bright-
ness may hurt user’s eyes.
• AUTO OFF (④): if you want the illuminator to
switch off automatically, press this button until 15,
30 or 60 minutes delay is set. After this period of
time, the light will turn off. You have to press the
ON-OFF button to turn it on again.
9.3 Brightness adjustment
Use the brightness adjustment dial ⑤ on the left side
of the microscope to increase or decrease the light
intensity on the specimen. (Fig. 14)
Fig. 12
③
④
①
Fig. 13
②
Fig. 14
⑤

Page 15
9.4 Adjust the observation head
Loosen the lock-screw ①, turn the observation head
to a comfortable position for observation, and then
lock the lock-screw. (Fig. 15)
9.5 Adjust the interpupillary distance
Hold the right and left parts of the observation head
using both hands and adjust the interpupillary distan-
ce by turning the two parts until one circle of light can
be seen. (Fig. 16)
9.6 Diopter adjustment
Adjust the ne focusing knob to get the image sharp
and clear while observing with your right eye, then
turn the left diopter ring ② to obtain a sharp and clear
image also with the left eye. (Fig. 17)
The highpoint eyepieces allow the user to wear glas-
ses
• NOTE: For the optimal parfocality of the image,
it’s suggested to wear your glasses during the
normal use of the microscope.
Fig. 15
①
Fig. 16
Fig. 17
②

Page 16
9.7 Use of eyeshields
• Use with eyeglasses
Fold rubber eyeshields with both hands. Folded
eyeshields avoid scratching the lenses of eyeglas-
ses. (Fig. 18)
• Use without eyeglasses
Raise eyeshields and observe at the microscope
placing eyes to the shields, avoiding external light to
disturb the observation. (Fig. 19)
9.8 Focus tension adjustment
Turn the tension adjustment knob to get a suitable
tension for the focus system. (Fig. 20)
• NOTE: if the tension is too loose, the stage could
go lower by itself or the focus easily lost after ne
adjustment. In this case, rotate the knob in order
to increase the friction.
9.9 Focus stop lever
Using the focusing knobs focus the specimen with a
4x or 10x objective. Then rotate the focus stop ①in
order to block the height of the stage. This simplies
the next focusing operations. (Fig. 21)
The focus stop lever is also useful to avoid accidental
contacts between objective and specimen.
Fig. 18
Fig. 19
Fig. 20
Fig. 21
①

Page 17
9.10 Stage
Stage accepts standard slides 26 x 76 mm, thickness
1,2 mm with coverslide 0,17mm. (Fig. 22)
It is possible to place two slides side by side on the
stage.
1. Open the spring arm of the slide holder ① and
place from the front the slide on the stage.
2. Gently release the spring arm of the slide holder.
• A sudden release of the spring arm could
cause the falling of the slide.
9.11 Condenser centering
1. Place the specimen on the stage, insert 10x
objective into the light path and focus.
2. Insert the front lens of the swing-out condenser
①. (Fig. 23)
3. Rotate the eld diaphragm ring ② in the direction
showed by the arrow, to fully close the diaphragm.
4. Rotate the condenser height adjustment knob ③
to focus the edges of the diaphragm.
5. Rotate the two centering screws ④to bring the
bright spot in the center of the eld of view.
6. Gradually open the diaphragm. The condenser is
centered when the diaphragm image is symme-
trical to the eld of view.
7. In normal use, open the diaphragm until it cir-
cumscribes the eld of view.
Effectofelddiaphragm
Field diaphragm adjusts the illuminated area to
obtain a high contrast image.
Set the diaphragm according to the objective in use
until it circumscribes the eld of view, in order to eli-
minate unnecessary light to eyepieces. (Fig. 24)
Aperture diaphragm
• The Numerical Aperture (N.A.) value of the aper-
ture diaphragm affects the image contrast. Incre-
asing or reducing this value one can vary reso-
lution, contrast and depth of focus of the image
• With low contrast specimens set the numerical
aperture value ⑤ (printed on the condenser ring)
to about 70%-80% of the objective’s N.A. (Fig.
25). If necessary, remove one eyepiece and, lo-
oking into empty sleeve, adjust the condenser’s
ring in order to obtain an image like the one in
g. 26.
Example:withobjectivePLAN40x/0.65setthe
scaleto0.65x0.8=0,52
Fig. 22
①
Fig. 23
Fig. 24
Fig. 25
⑤

Page 18
9.12 Use of oil immersion objective
1. Focus the specimen with a low power objective.
2. Lower the stage (remembering to lock the coarse
upper limit knob).
3. Put a drop of oil (provided) on the area of the
specimen to be observed. (Fig. 27)
• Make sure that there are no oil bubbles. Air
bubbles in the oil damage the image quality.
• To check for bubbles: remove an eyepiece, ful-
ly open the aperture diaphragm and observe the
objective exit pupil. (The pupil must be round and
bright).
• To remove the bubbles, gently move the nose-
piece to the right and left to move the immersion
objective a few times and allow the air bubbles
to move.
4. Insert immersion objective.
5. Return the stage to the upper focusing point and
obtain an optimal focus using the ne focus knob.
6. After use, gently remove the oil with a soft paper
towel or a lightly moistened optic paper with a
mixture of ethyl ether (70%) and absolute alcohol
(30%).
• Theimmersionoil,ifnotimmediatelycleaned,
could crystallize creating a glass-like layer.
In this situation the observation of the speci-
menwouldbedifcultifnotimpossibledue
to the presence of an additional thickness on
the objective.
Fig. 27
Fig. 26
IRIS DIAPHRAGM
FIELD OF VIEW
30-20%
70-80%

Page 19
9.13 Use of ALC system
1. Adjust the desired brightness through the eyepie-
ces using the light intensity dial (chapter 9.3).
2. Press the MEM key ① to store this setting (Fig.
28). The light on the microspoe will turn off for
some seconds, the will turn on again.
• The settings could not be working when the
light intensity is too low or too high. This is
not a defect.
3. The LED ② of the AUTO key ③ will light up to
show that the system is now active.
4. Now the system will automatically adapt the
brightness to the eyepieces when an objective is
changed, when the aperture diaphragm is used
or when another specimen is placed on the sta-
ge.
5. Pressing the AUTO key, the ALC system will be
disabled, but keeping in memory the setting of
the illumination previously done.
6. Pressing the AUTO key again the setting is re-
called.
• When ALC system is active the light intensity
dial is not active.
• To store a new setting, repeat step 1 and 2. This will
overwrite the old setting and will store a new one.
Only for motorised version
9.14 Nosepiece rotation
1. To change magnication it is possible opera-
te on the nosepiece movement buttons located
on the right side of the frame. (Fig. 29). Orange
button ④ rotates the nosepiece clockwise, while
the blue button ⑤ rotates the nosepiece counter-
clockwise.
2. As an alternative it is possible operate on the
right and left mouse buttons.
9.15 Focusing
Focus motor is activated through the mouse wheel.
Front or rear rotation of the mouse wheel raises or
lowers the stage. (Fig. 30)
9.16 Stage
1. Stage is moved through the mouse movement.
A mouse movement to the front or to the back ①
causes a stage movement of the stage along the
Y axis, while a left or right movement ② causes
a stage movement of the stage along the X axis.
(Fig. 31)
2. It is always possible, however, operate on the
translation knobs of the stage for a manual mo-
vement.
Fig. 28
①
③
②
Fig. 29
④
⑤
Fig. 30
Fig. 31
①
②
①
②

Page 20
10. Useofuniversalcondenserforbrighteld/darkeld/phasecontrast
Universal condenser provided with B-1000 allows observation in brighteld, darkeld and phase contrast.
10.1 Brighteldobservation(BF)
1. Rotate the condenser turret to insert the “BF” position.
2. Now repeat the steps described in the procedure “Summary of brighteld observation procedures” at page 10.
10.2 Darkeldobservation(DF)
1. Rotate the condenser turret to insert the “DF” position.
2. Open the aperture diaphragm.
3. Place a specimen on the stage and focus.
4. Observing into eyepieces raise or lower the condenser until a homogeneous illumination of the specimen can
be achieved, thus obtaining a proper darkeld effect.
• Darkeldrequiresahugeamountoflight.Switchingfromdarkeldtobrighteld,onecouldbedazzled.
Do not keep your eyes on the eyepieces when moving the condenser turret from DF to BF.
• “Dry”darkeldobservation,thatis,withouttheuseofoil,isonlypossiblewithobjectiveswithN.A.
lower than 0.7.
• Observingindarkeld,itmaybenecessarytoraisethecondenserfromthenormalpositiontoobtaina
more homogeneous illumination. This is not a defect.
Fig. 32 Fig. 33
Fig. 34 Fig. 35 Fig. 36
Observation mode Condenser turret position
Brightfield BF (Fig. 32)
Darkeld DF (Fig. 33)
Phase contrast 10x 10/20 (Fig. 34)
Phase contrast 20x 10/20 (Fig. 34)
Phase contrast 40x 40 (Fig. 35)
Phase contrast 100x 100 (Fig. 36)