Olympus FH User manual

olympus microscope
scanned by J. G. McHone •t .
21 Nov 09 NOT FOR SALE zns ructzons
Olympus -Tokyo

scanned by J. G. McHone 21 Nov 2009
forpersonal use
only, not for sale
OLYMPUS MICROSCOPE MODEL FH
The new Olympus FH Microscope was made in response to awide
demand
for amicroscope with perfect illuminator. This Microscope, then,
is an improvement on the
highly popular FMicroscope, whose fine adjustm-
ent is 0.0005 mm, and whose heads, stages, and lenses are interchangeable.
The Olympus
FH Microscope, therefore, is amicroscope we can recom-
mend
to the public with pride and confidence. The Olympus Optical Co.,
ltd,. we might add, was established in 1919, and since has been turning
out optical products exclusively.
OUTSTANDING FEATURES OF OLYMPUS FH MICROSCOPE
1. Perfect Built·in Illuminator.
Centering need only
be done once regardless of changes in magnification.
2. 0.0005 mm Fine Adjustment.
This microscope boasts
an ultra-sensitive fine adjustment system, which
is the first of its kind in the world.
Focusing is
easy, and, what is more, the thickness of the specimen can be
measured by the sensitivity scale.
3. Interchangeability of Head and Stage.
This microscope is available in
any desired head·stage combination.
Furthermore, the
head and stage can be changed easily and speedily.
4. Plan Achromatic Objectives.
The plan achromatic objectives haye been so designed that there is no
spherical aberration. Furthermore, the field of photomicrographic work
can
be widened by using low magnification plan aChromafic objectives.

STANDARD COMBINATIONS OF FH MICROSCOPE
1. Binocular
Body
Type Eyepiece
Stage Condenser Magnifications
FHF -Bi -IP7X, WFlOX FS
N.A.I.25
28X·1500X
PI5X
(paired)
FHF -Bi - II
P7X, WFIOX
FS
N. A. 1.25
28X - 2000X
PI5X, K20X
(Paired)
FHF-Bi -III
FS N. A. I. 25 28X - 2000X
2.
Trinocular Body
Typa Eyepiece
Stage
Condenser
Magnifications
FHF· Tr -IP7X. WFlOX FS N.A.I.25
28X ·1500X
PI5X
(Paired)
P.7X, P.lOX
P. 15X
(one each for
photomicro.)
FHF - Tr - II
P7X, WFIOX FS N. A. 1.25
28X - 2000X
PI5X, K20X
(paired)
P.7X, P.IOX
P. 15X
(one eac
10<
photomicro.)
FHF - Tr-III PJan4X FS N. A. 1.25
28X -2000X
P1anll'l
Plan40X
P.7 , f':--.IOX
Plan ;(lOX
(oj mmersion)
P. 15X
(one each for
photomicro.)
2

NAME OF PARTS
Arm ...,
Condenser Centering Screw ---~......J
Coarse Adjustment Handle ----_
Fine Adjustment Handle -----
Coarse Adjustment Stopper ------
Lamp Centering Screw
Socket Clamp Screw
SPECIFICATIONS
'r---------- Eyepiece
,-------- Prism Housing
... - .... ,.---- Revo!vinS NosepIece
~---- Objective
Stage
r------ Iris Diaphragm Lever
"------ Filter Holder
'------- Stage Stopper
Condenser Vertlca I
.---------- Movement Handle
Projection Lens
Sase
Field
Diaph"~gm
Body Tube:
Revoll/jng Nosepiece:
BUlb:
Transformer:
Filter:
Ught Source:
Focusing;
Coarse Adjustment:
Binocular Heed -, 45
ft Inclination, rotatable through
360
0horizontally.
Trinocular Head Binocular part 45
ft
inclination, rotatable through
360" horizontally.
Quadruple (ball bearing
system) indicator for obJectives' position.
6V 5A TB-l (clear)
Te. IOOV. IIOV. 220V. 240V aYallable.
32.5 ~ mm (blue)
Bulb centerIng adjustment type; compensatIon lens Shifting system (for
low, medium and high light value)_
Vertical movement stage; co-axial coarse and fine adjustment handles..
Rack-tlnd-pinion with
stopper; range of operation; 32.5mm
3

OPTICAL PATH
~--
<-----~ ---- ----
LL_~ __ /
Fine Adjustment: Balanced spring-tension system; range of vertical movem@nt: 1.2mm;
scale; calibrated In unit of O.OOlmm.
Condenser Focusing System: Rack-aod-pinion ty~e; range of vertical movement: 23.5mm;
centering device.
Condenser: N.A. 1.25 condenser with external diameter of 36.8mm and featuring
varlabre iris-diaphragm and filter holder.
Stage: SCluare Coaxial Mechanical Stage (double-handle) F'S .- .... easy to detach
and replace the stages, as it 15 dovetailed to the body.
Special Attachments: The Round Revolving Stage Model RS.
The SCluare Coaxial Mechanical Stage (single-handle) Mod@1 es.
The Square ReYolvlng Coaxial Mechanical stage (single-handle) model erS,
4

STRUCTURE OF PARTS
A. MICROSCOPE BODY
Arm, Focusing Mechanism and Condenser:
The arm is secured firmly to the base by means of a support, and. in turn, it supports such
parts as the focusing mechanism, stage. condenser, revolving nosepiece and the head. furthermore.
It is shaped uo facitrtate carrying around.
The focusing mechanism has such built in unrts 35 the coarse adjustment and fine adjustment
units and the coarse adjustment stopper. The coarse adjustment handle and the fine adjustment
handle are coaxial. In other words. the large handle is used for coarse adjustment and the small
handle for fine adjustment. The stage will move nO hIgher than the height obtained by manipul
ating the coarse adjustment handle, provided that the coarse adjustment stopper is tightened.
In othe words. when the stage is lowered and again brought up toward the objecti\le. it will go
no higher than the stop position. This not onl)l pre~ents contact of the specimen and the objective
but also facilitate5 focusins. The condenser mount. which i5 of the rack-and-plnlon type, has it
recei~er with a dovetail groove. ThB condenser mount l:;crew is used for adjusting the optIcal axis.
~ Adjustment Handle- .... +.,..~!LJ
I Adjustment Handle
----Condenser Fitting Screw
'---- Iri5 Diaphr.8gm Lever
'---- Filte, Holde,
"------ Condenser Centering Screw
'------- Stege Stopper
Coarse Adjustment Stopper
5

B. MICROSCOPE HEAD
The mitroscope head of Model EH microscope is inclined 45 degrees from the horizontal plane
so as to enable microscopic examination in the most natural posture and with is minimum of fatigue.
As mentioned previously, the standard height of this instrument Is 380mm from the desktop.
This is ex.actly the height required for the average person to conduct microscopic examination In
the
most natural sitting posture, Thus, aspecial desk is abSOlutely unneceSSary when this MOdel
EH microscope is used.
The following four types of microscope h~ad are
available;
1. Binocular head (Bi)
2. Trinocular head (Tr)
3. Upright head for photomicrography
4. Monocular head
To mount the mu;:roscope head, turn the
knurled rinB of the microscope fix.ing screw.
This witt fix the head properly to the arm and;
at the sam~ time, secure precise optical align-
ment.
Microsc.ope h@:ad
fixing guide
360
0Ranso
of
adjustment
of direction
01 micro
scope head
B·1 Binocular Head
I
Microscope head filcing ring
Ttle above he.ads, with the exception of the
upright head for photomicrography, which remains
In a fix@d position, can be rotated through 360
0
horizontally,
The light from the objective reach@$ the eyepieces after being split into two by the semi.
transmissIve prism. This head was designed taking Into consideration the Characteristic function
of the human eyes_
That is to say. the right and left optical axis are slanted slightly toward the center. Furthermore,
the eyepieces can be adjusted according to the user's inter-pupillarv distance. This is done by
sHpping out the dovetailed eyepieces. The inter-pupillary distance Is shown On the scale between
the two eyepieces. This operation is carr@d eye-width adjustment. The scale readinBs are based
on BiWF lOX eyepie:ces. Since one e~e is often weaker than
the other, this tube is equipped with a diopter adjustment
ring on the left eyepiece. When the dioptric adjustment ring
is set at 2.ero, and the inter-pupillary distance scale reads
62, the mechanical tube measures aDoul 160mm long.
6

B·2 Trinocular Body
The trinocular head consists of binocular head and upright head for photomic.rography. The
photo,trapller can actually see what he is photographing since it inCO(PO~iJtes a mechanism to
switch the light pateh alternately for direct observation and photomicrographic work. The binocular
head is equipped with iJ dioptric adjustment ring and an Inter-pupHlary distance scale,
Straight tube for
photomicrography
prism shiWng knob
When the prism shlftlng knob is pUlled outward
as shown in the above photo. the prism locks into
position (0) diagram) and the path of rays is direc-
ted to lhe upright head for photomicrographic work.
A PUSh in of the knob almost instantly returnS
the prism to its original position (@ diagram).
ThIs unique mechanism (one-knob operation)
permits direct observation and photomicrographic
work alternately with aminimum loss of time.
rn Path of rays at the time
W of photomicrography
00 Path of rays at the time
of direct observation
Inter-pupillary distance scale
Dioptric adjustment ring
set at zero
B-3
Upright head for photomicrography
In case of microscopes featuring monocular
inclined head or binocular head, the standard
microscope head must be interchanged with
an upright head when conducting photomicro·
graphic work. The photomicrographic appara·
tus must be mounted on this upright head.
B-4 Monocular Head
7

C. STAGE
There Ire four types of stages available for- Model F'H:
oSquare coaxial mechanical stage (double handle) FS:
oSquare revolving coaxial mechanical stage (single handle) erS
oSquare coaxial mechanical stage (single handle) CS
oRound revolving stage RS
The square cOClxial mechanical stage fS is standard equipment of the fH Microscope.
C·l Square Coaxial Mechanical Stage FS
ThIs square stage can be detached at will since it is dovetailed to the main body. The specimen
can be moved by means of handles found on both sides of the stage. For forward and backward
movement of the specimen, a large handle (raCk-aod-pinion) is used, and a small handle (lead
screw) is used for lateral movement Th{J surface of the stage can be made level bY removing
the Clip.
Two slide glasses can be placed on the specimens side by side. This is extremely convenient
when comparing two specimens at the same Ume.
Lateral movement handle
Lateral movement scale ----'l---=
forward and backward movement handle
Forward and backward movement scale
Lateral movement handle vernier
Forward and backward movement handle
8

P. lOX
Binocular head
P15X
Eyepieces
Photo lenses
~
Monocular inClined head
MODEL FH
VARIED COMBINATION
OF PARTS
Ob)ecti\lu
Round stage RS
Abbe condensers
N A 1.25

-
P. 7X
Trlnocular head
Ugright head
Square mechanical stage
(single handle) CS
N.A.I.40
Square revolving mechanical stage
(single handle) C,S
SWing-out N. A. 0.25-0.9
Square mechBnical stage
(double handle) FS

C-2 Square Revolving Coaxial Mechanical Stage (single handle) CrS
This stage is fixed firmly to the bOdY of the microscope. and on Its surfaee, there is a cross
movement clip. The specimen is shifted by means 0" a coa;a:iar type forward and backward
movement handle and lateral movement handle on the right side of the stage. The tormer Is
operated by means of arac.k-and-pinion. whereas the latter Is manIpulated by means of alead
screw.
The workIng ranse of the handles are:
Forward and backward 27mm
Lateral _ _ 70mm
Each handle has ascale (0-30. 40-110) and a vernier.
Rotation; After st!'tting the slide glass in position, the center of the slide glass is brought
Into line with the optical axis. Then the sta86 j!i rotated horizontally. The stage rotates about
220· to clockwise, and about 200:. to counter-cloc:kwise. Rotation can be stopped by means of
the stage stopper,
Rotating 220·
Lateral movement handle
~
Forward and backward
movement handle
C-3 Square Coaxial Mechanical Stage (single handle) CS
This mechanical stage can be operated by a single handle which consists of a rack-aod-pinion
for forward and backward movement. and alead SCrew for lateral movement
Lateral movement scale
Forward and backward
movement handle
Forward and backward movement scale
lateral movement t1andle
11

C-4 Round Revolving Stage RS
The round revolving stage consists of two stages. The lower stage is fixed to the stage
mount On the main body. The upper stage slides over tne lower stage horizontally.
Sliding Is done by operating the left- and right- side fine adjustment handles by turns.
Furthermore, the upper stage can be rotated through 360" horizontally by pressing the knurl part
on the rim of the stage.
Clip
Stage fine movement handle
How to Fix Stage to the Body
The Olympus FH stases have been so made that they can be easily fixed to the main bodY.
The four stagos rs. CrS, CS and RS. eaen of which is used fer aspecifiC purpose _are
Jnterchangeable.
1. First of all, the condenser is lowered as far as possible by means of the condenser handle.
2.
The stage mount is also lowered l!I$ far as possible by turning the coarse adjustment handle,
3. In the case of FS. CrS and CS stages, the clip is moved forward as far as possible by
manipulating the forward and backward maven lent handre.
4. Next, the stage guide (,A) is slowly inserted Into that of the main body (8) with the
lock-rever up. The stage must be so Inserted that it is parallel with the main bodY, Care
must be taken not to crash the top lens of the condenser with the stage during this
operation.
5. Finally, the stage firmly fixed to the main body by means of the rock-lever.
(A)
lock-lever
12
(B)

D. REVOLVING NOSEPIECE
The revolvlllB nosepiece of Model FH features a ball-alld-socket revolving system with four
objective mounts. A knurled rillS is provided for slip-free and smooth manipulation of the
nosepIece. By means of the large built-in spril'lg and groove, the objll:!e:.tive s.ets into Position
ac.curately. maintaining proper optical alignment. ihe upper 'Surface of the (evolver is marked
with the letters A, 5, C, Din order to Show where tne objectives s,hould be fixed. 'A' i5 far 4X,
'8' fOr lOX. 'C' for 40X, and '0' for lOOX. In this way, the position of the objectives do not
change. Furthermore, the observe .. can tell which objective is being u5ed while conducting
observation.
B
(lOX)
A
(4X)
E. BASE AND ILLUMINATOR
Letter specifying position
of differ8nt objective
Tile base not only supports the microscope but also has built-in illuminator. The light source
unit is fixed to the base by means of fitting screws. Switch over the Or'st compensation. tens
according to the mCli811ification of the objective being used.
The compensation lens shiftll1g lever Is used for the following purposes.
l (Low) objective 4X or lass
M (Medium) objective lOX and 20X
H (High) objective 40X or mOre
Socket Clamp 5cr'ew
lamp Centering Screw
Field Stop
13
Projection' Lens

lens
(Low)
L- __ M (Medium)
(4X or '.ss)
6V.5A
\ I ~
.~----J f '
1-:--<. i I·_·-J I ~
ICOlYJ I~ -/ ')
Lr--j-<-----------'L ---J
Field stop
For low magnification
For high magnification (40X or mOl'"e)
EFFECTIVE ILLUMINATION
PriOr to conducting microscopy, it is of very importance to check whether appropriate and
effecth"e lightinS is directed to the specimen. That is to say, the observer should see if the ray
of light emitted from the illuminator Or mirror follows a central path leadins to the obiective and
eyepieces.
This can be done by look ins into the- eyepiece tube after roughly focusing a40X
objective or lOX. objective and remo\lling the eyepiece. If the light from the light source unit
illuminates throughout the lens surfac.e evenly without: any eccentricity, it means that proper.
effective lighting is beins rendered. E\lIen if the light is not eccentric., only the center of the lens
is often illuminated brightly. This means that the condenser is not in the proper position. This
can be corrected by adiusting the position of the condenser by moving it up and down. However.
it should be notea that inaccurate focusing produces the same phenomenon.
Even the slightest mistake can throw the optic path off center. To illustrate, the observer
should not even touch the mirror since it results ifl poor illumination. Furthermol'e, wnen the
image appearS to move off the center while manipulating the fine adjustmeflt handle in the COurse
of securing focus. it means that the path of rays is not aligned propel'ly. In this case, lighting
must be readjusted in accordance with the fore,going procedures.
CENTERING OF LIGHT
Centel'"ing of light from the light SOUl'ee is essential to ordinary micl'oscopy and mlcropl'loto·
sraphy, The followl"g procedul'"es permit to Obtain soo(J centering of ligl1t.
1. Focus the specimen with lOX objective.
2. Close both Il'i$ diaphragm of tne condenser and field stop of the illuminator, Bring ~Ield stop
(8 small circle of stop-lisht) into focus by moving the condenser up and down.
14

3. Set field stop (stop-light) at the center of the field by manIpulating two condenser centering
screws.
4. Open field stop slowly so that an entire field can be seen.
5. Remoye
the eyepiece from the e}'epiece tube, and open iris diaphragm slowlY, While looking
into the eyepiece tube, so that an entire field can be lighted.
6. By adjusting the lamp centering screws (2 screws) of the lamp house, set the image of
filament at the center of the field.
7. Replace the eyepiece. and start your microscopic examination.
NOTES:
(1) When low magnification objective. particularly 4X objective, Is used, the field is often
illuminated unevenly. In this case, adjust lighting by one of the following manners.
(s) Loosen the socket clamp sCrew and move the socket back and forth slowly, while
looking
into the eyepiece tube, and fbe the socket at a position where an even light
can be obtained.
(b) Remove the condenser and replace It after takinB off the top tens. In other words.
use
the condenser without the top lens.
(c) Put ground glass on the projection lens of the illuminator.
(2) Condenser.
The
numerical aperture of condenser is 1,25 when 011 is used for oil immersion microscopy.
otherwise it is about N. A.I. 0 ...... 0.9, and N. A. 0.5 without the top lens.
PROCEDURE FOR ADJUSTING THE COARSE ADJUSTMENT HANDLE
Generally speaking, the coarse adjustment nandles are tight.
This. of course, means that movement is heavy. However. this
can be remedied easily by gripping the right-and left-side
handles as illustrated and turning them in the opposite direction
SImultaneously. In most cases, the coarse adjustment handles
should be
more or less tight rather than loose.
WORKING DISTANCE AND PAR·FOCAL
The revolving nosepiece to which the objectives are attached, has been so designed that as
long as one objective has been focused properly the others can be focused by means of the fine
adiustment handle. The distance from the tip of low magnificiltion objectlve to the specimen is
considerably 100l3er than that from high magnification objective. The distance is shortest in the
case of oil Immersion objective, When the reVOlving nosepiece is rotated, after focus is secured
with one of the objectives. to SWitch the magnification from one to the other, 1nr Instance, to
the oil immersion objective. there is no danger of the tip of the lens touching the specimen. In
other words, mictMcopic examination can be conducted immediately after manipulating the fine
adjustment handle.
15

Focus at first by utilizing lOX objective. The distance
between the objective and the microspecimen in this case will
be approximatelv 5.6mm. Even when lOQX objective is set
to viewing position. it will not collide with the microspecimen_
The distance will then be about O. t4mm.
INSTRUCTION FOR USING OIL IMMERSION OBJECTIVE
Before using oil immersion objective. secure focus by using low magnifIcation objective. Apply
a drop of cargille 011 or anIsole to the surface of the specimen, Also apply cargille oil to the oil
immersion objective_ As we have already mentioned, the oil immersion objective will not touch
the specimen when the revolving nosepiece brings it into position over the specimen. After
focusing the lens by means of tHe fine adjustment handle, microscopic examination can be carried
out without any loss of time.
After examination. cargille oil left on the oil- immersion objective must be thoroughly wiped
off with gauze dipped in xylene. Otherwise. the lens will SOOn become useless, Crean gauze is
sufficient to wipe off anisole.
Place a
drop of cargille oil or
anisole on the microspecimen
Apply carsitle oil to the tip
of the oiHmmerson oblctive
CAUTION
Microscopes are extremely allergic to moisture and dust. Since both moisture and dust are
found in most laboratories, microscopes should be kept in containers imme:diately after use.
If this is not possible, they should be covered with the enclosed vinyl dust cover.
As for objectives and eyepieces. it is best to keep them in desiccators. Falling thls. they
should be kept in cases containing such desiccants as silica 8el.
After the e)'epiece has been removed, the e,yeplece tube should be covered with the enclosed
cap. By
no means should a microscope be taken apart for repairs. This should be left to experts.
And, of COurse. microscopes must alwa,ys be kept clean. Fine dust on parts that cannot be
reached by the hand should be blown or wiped off by means of air blower or clean feather.
16

ACCESSORIES
Small Photomicrographic Apparatus, PM-6
The PM-6 IS a photomicrographic unit designed exclusively for taking full-size photographs on
a 35mm film. Fits all types of biological and metallur'gical microscopes of all makes.
o Shutter speed: S, 1-1/50 sec.
oSize of image; 24mmx.36mm
oSpBcimen can be observed whlte photographing
oHas insertion opening (or photo cell of photomicrographic
exposure meter'.
oAio'allable for all eyepieces except 5X power.
o Shock-proof shutter prevents shutter IJlbration and insures
no blurred pictures.
oMagnification on the film plane: about If3X of total
magnifications of microscope.
Phase Contrast Equipment, PA • PB
The bright field observation method of microscopic examination can not be used for examining
colorless, transparent specimens since such specimens lack contrast. The phase contrast equipment,
therefore, was designed to provide such specimens with COntrast and thus make examination of
tivlng bacteria, c-ells and tissue possible.
Two kinds of light pass through specimen namely, undeviated light and deviated light.
Deviated lIght is Mwave length slower than undevi.ated light. Contrast can be strengthened
through light interference by makins undeviated light !4- !Nave length faster or srower. This meth04
is highly convenient for observation ot the ecology of the specimen since it eliminates such trou·
blesome procedures as dyeing the specimen. The Olympus phase contrast equipment is highly
prized today since it has widened the scope of bIologIcal microscopic e)(:amlnatlon. What is more.
It is easy to handle.
Contents of Phase Contrast Equipment Set.
model PA
Phase Contrast Objectives for 4 different co-
ntrasts each with 4 different magnifications
(total of 16 objectives) :
PL lOX, 20X, 40X. 100X
(oil Immerson)
PLL lOX, 20X, 40X, 100X (oil immerson)
NH lOX, 20X, 40X, IOOX (oil immerson)
NM lOX, 20X, 40X, 100X (oil immerson)
Phase contrast turret condenser,
Centering Telescope (CT),
Green filter (outer diameter 32.5mm)
17

model PB
One
set of Pf1ase. Contra-s! Objectives of the
above mentioned 4 contr.ast~ (PL, PLL.1 NH.
NMJ.
Als:J contains Ph;;lse contrast turret condenser,
c.entering telescope (CT), and green filter
(outer diameter 32.5mm).
Note; Contr'3st; P'L Positive Low
PLL Positive low low
NH Negative High
NM Negative Medium
Lens Container
ThiS is a container specially made for miCfOScOPO l@nS8S. With a desiccant (silica gel) at the
bottom of the container, lenses can be kept completely free of moisture and dust. The plastic
top permits to check the lenses from outside without r'emolo'ing the top, and it Is very convenient
to keep the lenses in order"
Capacity: 7lense'S (up to 57mm hish)
3 filters (up to 33mm in dia. and 3.5mm thick)
Plan Achromatic Objectives
fllew and originally designed Plan Achromatic Objectives, completely free of spherical aberration,
are inCluded as the 5tandard set in the Olympus Mic:roscope FH-III, Furthermore, use of the low
magnification Plan Achromatic Objectilo'es will help broaden the field of photomicro,graphic work.
oPlan Achromatic Objectives: 1.3X, 2X, 4X, lOX, 20X, 40X, lOOX
oCondenser exclusively for low magnificatioll (1.3X and 2X Objectives) microscopy.
18

Dark field condenser DC
8rownian movement of celloid, etc. is hal'"dly lJisiblll:! in
bright field, but with aid of the dark field condenser, all mic
ros-cope s permit examination of such mOVE!ment in a dark
field. (Use lOOX oil-immer$iOn objective with iris diaphragm
with dark field condenser.)
Filar micrometer eyepiece OSM
Accurate me.asurE!ment of a minimum of O.OOOlmm is
fe.asiblE! by Inserting this eyepiece Into the tube of the
microcope. When mOl.Jntlng it on mOflOClJlar inclined he2ld.
binocular head Qr trinoculal" head, first remoIJe the auxiliary
lens on the bottom ~eetion, and insert the eye- piece into the
eyepiece sleeve,
Eyepiece Micrometer and Objective Micrometer
These are used to measure the size of specimens.
(1) Eyepiece Micrometer: A round Blass plate, lOmm/100, 19mm in diameter'.
(2) Objectl'ole Micrometer; Aslide glass - 1mm/lOO. 26 x 76mm.
19