OPTIKA MICROSCOPES B-190 Series User manual
Model
B-190 Series (B-191 / B-191S / B-192 / B-192S / B-193)
B-190TB series (B-190TB)
B-190 Series
INSTRUCTION MANUAL
Ver. 7.0 2019
Page 2
1. Warning 3
2. Symbols and conventions 3
3. Safety Information 3
4. Intended use 3
5. Overview 4
5.1 B-191 / B-191S / B-192 / B-192S / B-193 4
5.2 B-190TB 5
6. Unpacking 6
7. Assembling 6
7.1 B-191S / B-191 / B-192S / B-192 / B-193 6
7.2 B-190TB 7
7.3 Assembling the microscope 8
7.3.1 B-190 Series 8
7.3.2 B-190TB 9
7.4 Polarizing set (optional) 10
8. Use of the microscope 11
8.1 Switching on the microscope 11
8.2 Light intensity adjustment 11
8.3 Coarse focus tension adjustment 11
8.4 Stage 11
8.5 Adjust the interpupillary distance 12
8.6 Diopter adjustment 12
8.7 Use of oil immersion objective 12
8.8 Aperture diaphragm 13
8.9 Use of the polarizer (optional) 13
9. Microphotography 14
9.1 Cameras with projection lens 14
9.2 Reexcamera 14
10. Maintenance 15
11. Troubleshooting 16
Equipment disposal 18
Summary
Page 3
1. Warning
This microscope is a scientic precision instrument designed to last for many years with a minimum of
maintenance. It is built to high optical and mechanical standards and to withstand daily use. We remind you
that this manual contains important information on safety and maintenance, and that it must therefore be made
accessible to the instrument users. We decline any responsibility deriving from incorrect instrument use uses
that does not comply with this manual.
2. Symbols and conventions
The following chart is an illustrated glossary of the symbols that are used in this manual.
CAUTION
This symbol indicates a potential risk and alerts you to proceed with caution.
ELECTRICAL SHOCK
This symbol indicates a risk of electrical shock.
3. Safety Information
Avoiding Electrical Shock
Before plugging in the power supply, make sure that the supplying voltage of your region matches with the
operation voltage of the equipment and that the lamp switch is in o position. Users should observe all safety
regulations of the region. The equipment has acquired the CE safety label. However, users have full responsibility
to use this equipment safely. Please follow the guidelines below, and read this manual in its entirety to ensure
safe operation of the unit.
4. Intended use
For research and teaching use only. Not intended for any animal or human therapeutic or diagnostic use.
Page 4
5. Overview
5.1 B-191 / B-191S / B-192 / B-192S / B-193
FINE FOCUS
KNOB
PHOTO TUBE
(ONLY B-193)
INTENSITY ADJUSTMENT
DIAL
STAGE
OBJECTIVES
COARSE FOCUS
KNOB
CONDENSER X/Y MOVEMENT
KNOBS
SLIDE HOLDER
APERTURE
DIAPHRAGM
OBSERVATION HEAD
-) MONOCULAR (B-191 / B-191S)
-) BINOCULAR (B-192 / B-192S)
-) TRINOCULAR (B-193)
EYEPIECES
TENSION
ADJUSTMENT RING
Page 5
5.2 B-190TB
INTENSITY ADJUSTMENT
DIAL
FINE FOCUS
KNOB
COARSE FOCUS
KNOB
STAGE
X/Y MOVEMENT
KNOBS
SLIDE HOLDER
OBJECTIVES
DIGITAL HEAD
TABLET PC
EYEPIECES
TENSION
ADJUSTMENT RING
APERTURE
DIAPHRAGM
Page 6
6. Unpacking
The microscope is housed in a moulded Styrofoam container. Remove the tape from the edge of the container
and lift the top half of the container. Take some care to avoid that the optical items (objectives and eyepieces)
fall out and get damaged. Using both hands (one around the arm and one around the base), lift the microscope
from the container and put it on a stable desk.
Do not touch with bare hands optical surfaces such as lenses, lters or glasses. Traces of grease or other
residuals may deteriorate the nal image quality and corrode the optics surface in a short time.
7. Assembling
Once opened the box, the microscope parts are the following:
7.1 B-191S / B-191 / B-192S / B-192 / B-193
① Frame
② Observation head
monocular (B-191 / B-191S)
binocular (B-192 / B-192S)
trinocular (B-193)
③ Photo tube (only B-193)
④ Eyepieces
⑤ Objectives
4X / 10X / 40X / 100X (B-191/B-192/B-193)
4X / 10X / 40X / 60X (B-191S/B-192S)
⑥ Dust cover
⑦ Green lter
⑧ Power supply
⑨ Immersion oil (B-191/B-192/B-193)
⑩ Tension adjustment tool
③
④
⑤
⑥
⑦
⑧
①
②
⑨⑩
Page 7
7.2 B-190TB
① Frame
② Digital observation head
③ Eyepieces
④ Objectives (4X / 10X / 40X / 100X)
⑤ Dust cover
⑥ Green lter
⑦ Immersion oil
⑧ Power supply
⑨ Tension adjustment tool
⑩ Tablet PC power supply
⑪ OTG cable
⑫USB cable
⑬ Touch pen for Tablet PC
⑭ Tablet PC + keyboard
③
④
⑤
⑥
⑦
①
⑨
⑩
⑪
⑫
⑬
⑭
②
⑧
Page 8
7.3 Assembling the microscope
7.3.1 B-190 Series
1. Remove the dust cap from the microscope frame
and from the bottom of the observation head.
2. Insert the optical head above the stand and
tighten the screw. (Fig. 1)
• Hold the head with one hand during the lo-
cking in order to avoid that the head falls.
3. Insert both eyepieces into the tubes of the optical
head. (Fig. 2)
4. Insert the power supply jack in the socket placed
at the rear side of the microscope. (Fig. 3)
Only for trinocular head
5. Unscrew the protection cap mounted on the
photo port and screw the photo tube. (Fig. 4)
Fig. 1
Fig. 2
Fig. 3
Fig. 4
Page 9
7.3.2 B-190TB
1. Remove the dust cap from the microscope frame
and from the bottom of the observation head.
2. Insert the optical head above the stand and
tighten the screw. (Fig. 5)
• Hold the head with one hand during the lo-
cking in order to avoid that the head falls.
3. Insert both eyepieces into the tubes of the optical
head. (Fig. 6)
4. Fix the rotating part of the junction using the black
wing-nut ①. (Fig. 7)
5. Then hook the Tablet PC onto the 4 screws of the
junction and pull toward down to rmly lock the
Tablet PC in the holder. (Fig. 8)
• To unlock the Tablet PC proceed with the opposi-
te operation: push toward up and remove it from
the holder.
Fig. 5
Fig. 6
Fig. 7
Fig. 8
①
Page 10
6. Plug one side of the cable named CAME-
RA CONNECTION (USB + OTG) ② to the
digital head and the other side to the Tablet
PC. (Fig. 15).
7. Plug the cable named POWER SUPPLY
CONNECTION to the Tablet PC for battery
recharge.
Fig. 9
②
Your Tablet’s been set with the Rotation function disabled: this prevents any ipping of the Live View in order to
get a continuous and as large as possible view of your slide also when the Tablet is removed from the holder. To
enable this function again is very easy: you can activate the Rotation by swiping the screen on his bottom right
side and selecting Settings + Screen. Anyway, it’s not suggested to activate the function when the camera is in
Live View mode as it may give troubles when the camera runs at high resolutions.
The Tablet PC can be xed to a junction
7.4 Polarizing set (optional)
1. Place the polarizer on the light exit ①at the
base of the microscope. (Fig. 10)
2. Loosen the head xing knob ② and remove
the head from the microscope frame. (Fig.
11)
3. Insert the analyzer into the hole inside the
frame ③. (Fig. 12)
4. Put back the head into its original position
and lock the xing knob.
Fig. 12
③
Fig. 10
①
Fig. 11
②
Page 11
8. Use of the microscope
8.1 Switching on the microscope
Operate on the main switch ① placed in the rear side
of the microscope, moving the selector on “I” (Fig. 13)
8.2 Light intensity adjustment
Operate on the light intensity dial to increase or de-
crease the illumination intensity. (Fig. 14)
8.3 Coarse focus tension adjustment
• Adjust the tension using the provided tool.
The coarse knob tension is pre-setted in the factory.
To modify the tension according to personal’s needs,
rotate the ring ③ using the provided tool (Fig. 15).
Clockwise rotation increases the tension. If the ten-
sion is too loose, the stage could go lower by itself
or the focus easily lost after ne adjustment. In this
case, rotate the knob in order to increase the tension.
8.4 Stage
Stage accepts standard slides 26 x 76 mm, thickness
1,2 mm with coverslide 0,17mm. (Fig. 16)
1. Open the spring arm of the slide holder ② and
place the slide from the front on the stage.
2. Gently release the spring arm of the slide holder.
• A sudden release of the spring arm could cau-
se the falling of the slide.
Fig. 7
Fig. 13
Fig. 14
Fig. \5
Fig. 16
②
①
Page 12
8.5 Adjust the interpupillary distance
ExceptmodelsB-191/B-191S
Hold the right and left parts of the observation head
using both hands and adjust the interpupillary distan-
ce by turning the two parts until one circle of light can
be seen. (Fig. 17)
• The graduation on the interpupillary distance in-
dicator ①, pointed by the spot “.” on the eyepiece
holder, shows the distance between the opera-
tor’s eyes.
The range of the interpupillary distance is 48- 75 mm.
8.6 Diopter adjustment
ExceptmodelsB-191/B-191S
1. Look into the right eyepiece with your right eye
only, and focus on the specimen.
2. Look into the left eyepiece with your left eye
only. If the image is not sharp, use the dioptric
adjustment ring ②to compensate. (Fig. 18)
• The adjustment range is ±5 diopter. The num-
ber indicated on the adjustment ring gradua-
tion should correspond to the operator’s dio-
ptric correction.
8.7 Use of oil immersion objective
B-191 / B-192 / B-193 /B-190TB
1. Focus the specimen with a low power objective.
2. Lower the stage.
3. Put a drop of oil (provided) on the area of the
specimen to be observed. (Fig. 19)
• Make sure that there are no oil bubbles. Air
bubbles in the oil damage the image quality.
• To check for bubbles: remove an eyepiece, ful-
ly open the aperture diaphragm and observe the
objective exit pupil. (The pupil must be circular
and bright).
• To remove the bubbles, gently move the nose-
piece to the right and left to move the immersion
objective a few times and allow the air bubbles
to move.
4. Insert immersion objective.
5. Return the stage to the upper focusing point and
obtain an optimal focus using the ne focus knob.
6. After use, gently remove the oil with a soft paper
towel or a lightly moistened optic paper with a
mixture of ethyl ether (70%) and absolute ethyl
alcohol (30%).
• The immersion oil, if not immediately cleaned,
could crystallize creating a glass-like layer.
In this situation the observation of the speci-
menwouldbedicult(evennotimpossible)
due to the presence of an additional thickness
on the objective.
Fig. 17
Fig. 19
①
Fig. 18
②
Page 13
8.8 Aperture diaphragm
The Numerical Aperture (N.A.) value of the aperture
diaphragm aects the image contrast. Increasing or
reducing this value one can vary resolution, contrast
and depth of focus of the image.
Move the diaphragm ring ① (Fig. 20) on the value
corresponding to the objective in use. In this case the
optimal setting of the condenser is achieved.
It is possible, however, move the ring to lower or hi-
gher values to adapt the observation to personal pre-
ferences.
• With low contrast specimens set the numerical
aperture to about 70%-80% of the objective’s
N.A. If necessary, remove on eyepiece and, lo-
oking into empty sleeve, adjust the condenser’s
diaphragm in order to obtain an image like the
one in Fig. 21.
8.9 Use of the polarizer (optional)
1. Remove the specimen from the stage.
2. Looking inside the eyepieces, rotate the polarizer
until the darkest position is achieved.
3. Once the dark is achieved (“extinction” or “Cros-
sed Nicol” position) it is possible to begin the
observation.
Fig. 21
IRIS DIAPHRAGM
FIELD OF VIEW
30-20%
70-80%
Fig. 20
①
Page 14
9. Microphotography
9.1 Cameras with projection lens
1. Remove dust caps from icanera and projec-
tion lens.
2. Screw the projection lens to camera thread.
(Fig. 22)
3. Insert the projection lens into the photo
tube. (Fig. 23)
9.2 Reexcamera
1. Screw the “T2” ring (not provided) at the end
of the projection lens (M-173), then install
the whole onto the reex camera. (Fig. 24)
2. Insert the projection lens into the photo
tube. (Fig. 25)
Fig. 22
Fig. 23
M1
M1) Screw the Reex adapter to the “T2” ring (provided with the
Fig. 24
M2
M3) Properly install the Reex camera’s adapter
on the trinocular port
Fig. 25
Page 15
10. Maintenance
To think about when and after using the microscope
• The microscope should always be kept vertically when moving it and be careful so that no moving
parts, such as the eyepieces, fall out.
• Never mishandle or impose unnecessary force on the microscope.
• Never attempt to service the microscope yourself.
• After use, turn o the light immediately, cover the microscope with the provided dust-cover, and keep
it in a dry and clean place.
Electrical safety precautions
• Before plugging in the power supply, make sure that the supplying voltage of your region matches
with the operation voltage of the equipment and that the lamp switch is in o-position.
• Users should observe all safety regulations of the region. The equipment has acquired the CE safety
label. However, users do have full responsibility to use this equipment safely.
Cleaning the optics
• If the optical parts need to be cleaned try rst to: use compressed air.
• If that is not sucient: use a soft lint-free piece of cloth with water and a mild detergent.
• And as a nal option: use the piece of cloth moistened with a 3:7 mixture of ethanol and ether.
• Note: ethanol and ether are highly ammable liquids. Do not use them near a heat source, near sparks or
near electric equipment. Use these chemicals in a well ventilated room.
• Remember to never wipe the surface of any optical items with your hands. Fingerprints can damage the
optics.
• Do not disassemble objectives or eyepieces in attempt to clean them.
For the best results, use the OPTIKA cleaning kit (see catalogue).
If you need to send the microscope to Optika for maintenance, please use the original packaging.
Page 16
11. Troubleshooting
Review the information in the table below to troubleshoot operating problems.
PROBLEM CAUSE SOLUTION
I. Optical Section:
LED operates, but eld of view re-
mains dark. Power supply is unplugged. Connect
Brightness is too low Set brightness to a proper level
Dirt or dust is visible in the eld of
view. Dirt/dust on the specimen Clean the specimen
Dirt/dust on the eyepieces Clean the eyepieces
Image looks double Aperture diaphragm is stopped
down too far Open aperture diaphragm
Visibility is poor.
• Image is not good.
• Contrast is poor.
• Details are indistinct.
• Image glares
Revolving nosepiece is in an
incorrect position
Move the nosepiece to a click stop
Aperture diaphragm is too closed
or to open
Adjust aperture diaphragm
Dust or dirt on lenses (condenser,
objectives, eyepieces and slide) Clean thoroughly
For transmitted light observation,
the coverglass thickness must not
exceed 0.17mm
Use a coverglass with thickness 0.17mm
Focus is not even Slide holder is not flat. Move the specimen
to a flat position
One side of the image is out of focus. The nosepiece is not in the center
of the light path Turn the nosepiece to a click stop
The specimen is out of place
(tilted) Place the specimen at on the stage.
The optical performance of the
sample cover glass is poor Use a cover glass of better quality
II. Mechanical Section:
The coarse focus knob is hard to
turn. The tension adjustment collar is
too tight
Loosen the tension adjustment collar
The focus is unstable. The tension adjustment collar is
too loose Tighten the tension adjustment collar
III. Electric section
The LED doesn’t turn on. No power supply Check the power cord connection
The brightness is not enough The brightness adjustment is low Adjust the brightness
The light blinks The power cord is poorly con-
nected Check the power cord
IV. Observation tube
Field of view of one eye does not
match that of the other. Interpupillary distance is incorrect. Adjust interpupillary distance.
Incorrect diopter adjustment.Adjust diopter.
Your view is not accustomed to
microscope observation.
Upon looking into eyepieces, try looking at
overall field before concentrating on speci-
men range. You may also find it helpful to
look up and into distance for a moment
before looking back into microscope.
Page 17
IV. Observation tube
Field of view of one eye does not
match that of the other. Interpupillary distance is incorrect Adjust interpupillary distance
Incorrect diopter adjustment Adjust diopter
Your view is not accustomed to
microscope observation
Quando guarda il campione non focalizzi
lo sguardo in un unico punto ma guardi
l’intero campo visivo a disposizione. Peri-
odicamente distolga lo sguardo e guardi un
punto distante, dopodichè torni ad analiz-
zare il campione.
V. Microphotography
Image edge is unfocused To a certain extent it is due to ach-
romatic objectives features
To minimize the problem, set the aperture
diaphragm in a proper position
Bright spots appear on the image Stray light entering in the mi-
croscope through eyepieces or
camera viewfinder
Cover eyepieces and viewfinder with a
dark cloth
Page 18
Equipment disposal
Art.13 Dlsg 25 July 2005 N°151. “According to directives 2002/95/EC, 2002/96/EC and 2003/108/EC relating
to the reduction in the use of hazardous substances in electrical and electronic equipment and waste disposal.”
The basket symbol on equipment or on its box indicates that the product at the end of its useful life should be
collected separately from other waste. The separate collection of this equipment at the end of its lifetime is or-
ganized and managed by the producer. The user will have to contact the manufacturer and follow the rules that
he adopted for end-of-life equipment collection. The collection of the equipment for recycling, treatment and
environmentally compatible disposal, helps to prevent possible adverse eects on the environment and health
and promotes reuse and/or recycling of materials of the equipment. Improper disposal of the product involves
the application of administrative penalties as provided by the laws in force.
OPTIKA S.r.l.
Via Rigla, 30 - 24010 Ponteranica (BG) - ITALY Tel.: +39 035.571.392
OPTIKA
Spain
OPTIKA
USA
OPTIKA
China
OPTIKA
India
OPTIKA
Central America
®
®
®
®
®
®
Modelli
Serie B-190 (B-191 / B-191S / B-192 / B-192S / B-193)
Serie B-190TB (B-190TB)
Serie B-190
MANUALE DI ISTRUZIONI
Ver. 7.0 2019
This manual suits for next models
7
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