Optika Italy B-383 Series User manual

Ver. 2.1 2022
INSTRUCTION MANUAL
B-383 Series
Model
B-383MET

Page 2
Table of Contents
1. Warning 3
2. Safety Information 3
3. Package content 4
4. Unpacking 5
5. Intended use 5
6. Symbols and conventions 5
7. Instrument description 6
8. Assembling 8
9. Summary of transmitted light observation procedures 10
10. Use of the microscope in transmitted light 11
10.1 Turning on the instrument 11
10.2 Light intensity adjustment 11
10.3 Adjusting the interpupillary distance 11
10.4 Diopter adjustment 11
10.5 Coarse focus tension adjustment 12
10.6 Coarse upper limit lever 12
10.7 Stage 12
10.7.1 Use of the glass plate 12
10.8 Centering the condenser 13
10.9 Aperture diaphragm 13
11. Summary of reflected light observation procedures 14
12. Use of the microscope in reflected light 15
12.1 Turning on the instrument 15
12.2 Light intensity adjustment 15
12.3 Useofelddiaphragm(FS) 15
12.4 E󰀨ectsofelddiaphragm 15
12.5 Useofaperturediaphragm(AS) 16
12.6 Useofcolorlters 16
12.7 Use of polarized light 16
13. Microphotography 18
13.1 Use of C-mount cameras 18
13.2 Useofreexcameras 18
14. Maintenance 19
15. Troubleshooting 20
Equipment disposal 22

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1. Warning
This microscope is a scientic precision instrument designed to last for many years with a minimum of maintenance. It
is built to high optical and mechanical standards and to withstand daily use. We remind you that this manual contains
important information on safety and maintenance, and that it must therefore be made accessible to the instrument users.
We decline any responsibility deriving from incorrect instrument use uses that does not comply with this manual.
2. Safety Information
Avoiding Electrical Shock
Before plugging in the power supply, make sure that the supplying voltage of your region matches with the operation voltage
of the equipment and that the lamp switch is in o󰀨 position. Users should observe all safety regulations of the region. The
equipment has acquired the CE safety label. However, users have full responsibility to use this equipment safely. Please
follow the guidelines below, and read this manual in its entirety to ensure safe operation of the unit.

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3. Package content
① Microscopeframe
② ReectedlightIlluminator
③ Observationhead
④ Eyepieces
⑤ Objectives
⑥ Tensionadjustmenttool
⑦ Allenwrench
⑧ Colorlters
⑨ Analyzer+polarizer
⑩ Glassplate
⑪ Dustcover
⑫ Powersupply
①
③
④
⑤
⑥
⑦
⑧
②
⑨⑩
⑪
⑫

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4. Unpacking
The microscope is housed in a moulded Styrofoam container. Remove the tape from the edge of the container and lift the
top half of the container. Take some care to avoid that the optical items (objectives and eyepieces) fall out and get dam-
aged. Using both hands (one around the arm and one around the base), lift the microscope from the container and put it
on a stable desk.
Do not touch with bare hands optical surfaces such as lenses, lters or glasses. Traces of grease or other
residuals may deteriorate the nal image quality and corrode the optics surface in a short time.
5. Intended use
Standard models
For research and teaching use only. Not intended for any animal or human therapeutic or diagnostic use.
IVD Models
Also for diagnostic use, aimed at obtaining information on the physiological or pathological situation of the subject.
6. Symbols and conventions
The following chart is an illustrated glossary of the symbols that are used in this manual.
CAUTION
This symbol indicates a potential risk and alerts you to proceed with caution.
ELECTRICAL SHOCK
This symbol indicates a risk of electrical shock.

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PHOTO/TV PORT
DIOPTER
ADJUSTMENT RING
OBSERVATION
HEAD
7. Instrument description
SLIDE HOLDER
ANALYZER
EYEPIECES
CONDENSER
STAGE
REVOLVER
OBJECTIVES
X/Y MOVEMENT
KNOBS
CONDENSER
CENTERING
SCREWS
APERTURE
DIAPHRAGM
LED FOCUS LEVER
POLARIZER
TENSION
ADJUSTMENT
RING

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BRIGHTNESS ADJUSTMENT
KNOB
REFLECTED LIGHT
ILLUMINATOR
FIELD DIAPHRAGM
(FS)
APERTURE
DIAPHRAGM (AS)
Opposite side
FOCUS LOCK LEVER
CONDENSER HEIGHT
ADJUSTMENT KNOB
FINE FOCUS KNOB
COARSE FOCUS KNOB
LED HOUSING

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8. Assembling
1. Insert the reected light illuminator ① on the frame and lock
the locking knob ② with the provided Allen wrench. (Fig. 1)
2. Connect the illuminator cable ③ to the socket ④placed on
the back side of the frame. (Fig. 2)
3. Insert the optical head above the epi-illuminator and tighten
the locking screw. (Fig. 3)
• Hold the head with one hand during the locking in order
to avoid that the head falls.
FFig. 1ig. 1
FFig. 2ig. 2
FFig. 4ig. 4
FFig. 3ig. 3
①
②
③
④
4. Insert both eyepieces into the tubes of the optical head. (Fig.
4)

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5. Insert the polarizer. (Fig. 5)
6. Remove the protection cap and insert analyzer. (Fig. 6-7)
7. Insert the power supply jack in the connector placed on the
back side of the frame. (Fig. 8)
FFig. 5ig. 5
FFig. 6ig. 6
FFig. 7ig. 7
FFig. 8ig. 8

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9. Summary of transmitted light observation procedures
(Used commands) (Chapter)
Main switch
Nosepiece
Coarse and fine focus knobs
Observation head
Diopter adjustment ring
Aperture diaphragm
Nosepiece
Brightness adjustment knob
Coarse and fine focus knobs
Put on “I” the main switch and adjust brightness
Insert 10x objective into the light path
Focus the specimen
Begin observation
Adjust aperture diaphragm
Insert the desired objective into the light path and focus the
specimen
Adjust interpupillary distance
Adjust diopter
Adjust brightness
10
7
7
7
7
10
10
10
10
Slide holder
X/Y movement knobs
Place a specimen on the stage 10
7
Brightness adjustment knob 10