Optika Italy B-383 Series User manual

Ver. 2.1 2022
INSTRUCTION MANUAL
B-383 Series
Model
B-383MET

Page 2
Table of Contents
1. Warning 3
2. Safety Information 3
3. Package content 4
4. Unpacking 5
5. Intended use 5
6. Symbols and conventions 5
7. Instrument description 6
8. Assembling 8
9. Summary of transmitted light observation procedures 10
10. Use of the microscope in transmitted light 11
10.1 Turning on the instrument 11
10.2 Light intensity adjustment 11
10.3 Adjusting the interpupillary distance 11
10.4 Diopter adjustment 11
10.5 Coarse focus tension adjustment 12
10.6 Coarse upper limit lever 12
10.7 Stage 12
10.7.1 Use of the glass plate 12
10.8 Centering the condenser 13
10.9 Aperture diaphragm 13
11. Summary of reflected light observation procedures 14
12. Use of the microscope in reflected light 15
12.1 Turning on the instrument 15
12.2 Light intensity adjustment 15
12.3 Useofelddiaphragm(FS) 15
12.4 E󰀨ectsofelddiaphragm 15
12.5 Useofaperturediaphragm(AS) 16
12.6 Useofcolorlters 16
12.7 Use of polarized light 16
13. Microphotography 18
13.1 Use of C-mount cameras 18
13.2 Useofreexcameras 18
14. Maintenance 19
15. Troubleshooting 20
Equipment disposal 22

Page 3
1. Warning
This microscope is a scientic precision instrument designed to last for many years with a minimum of maintenance. It
is built to high optical and mechanical standards and to withstand daily use. We remind you that this manual contains
important information on safety and maintenance, and that it must therefore be made accessible to the instrument users.
We decline any responsibility deriving from incorrect instrument use uses that does not comply with this manual.
2. Safety Information
Avoiding Electrical Shock
Before plugging in the power supply, make sure that the supplying voltage of your region matches with the operation voltage
of the equipment and that the lamp switch is in o󰀨 position. Users should observe all safety regulations of the region. The
equipment has acquired the CE safety label. However, users have full responsibility to use this equipment safely. Please
follow the guidelines below, and read this manual in its entirety to ensure safe operation of the unit.

Page 4
3. Package content
① Microscopeframe
② ReectedlightIlluminator
③ Observationhead
④ Eyepieces
⑤ Objectives
⑥ Tensionadjustmenttool
⑦ Allenwrench
⑧ Colorlters
⑨ Analyzer+polarizer
⑩ Glassplate
⑪ Dustcover
⑫ Powersupply
①
③
④
⑤
⑥
⑦
⑧
②
⑨⑩
⑪
⑫

Page 5
4. Unpacking
The microscope is housed in a moulded Styrofoam container. Remove the tape from the edge of the container and lift the
top half of the container. Take some care to avoid that the optical items (objectives and eyepieces) fall out and get dam-
aged. Using both hands (one around the arm and one around the base), lift the microscope from the container and put it
on a stable desk.
Do not touch with bare hands optical surfaces such as lenses, lters or glasses. Traces of grease or other
residuals may deteriorate the nal image quality and corrode the optics surface in a short time.
5. Intended use
Standard models
For research and teaching use only. Not intended for any animal or human therapeutic or diagnostic use.
IVD Models
Also for diagnostic use, aimed at obtaining information on the physiological or pathological situation of the subject.
6. Symbols and conventions
The following chart is an illustrated glossary of the symbols that are used in this manual.
CAUTION
This symbol indicates a potential risk and alerts you to proceed with caution.
ELECTRICAL SHOCK
This symbol indicates a risk of electrical shock.

Page 6
PHOTO/TV PORT
DIOPTER
ADJUSTMENT RING
OBSERVATION
HEAD
7. Instrument description
SLIDE HOLDER
ANALYZER
EYEPIECES
CONDENSER
STAGE
REVOLVER
OBJECTIVES
X/Y MOVEMENT
KNOBS
CONDENSER
CENTERING
SCREWS
APERTURE
DIAPHRAGM
LED FOCUS LEVER
POLARIZER
TENSION
ADJUSTMENT
RING

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BRIGHTNESS ADJUSTMENT
KNOB
REFLECTED LIGHT
ILLUMINATOR
FIELD DIAPHRAGM
(FS)
APERTURE
DIAPHRAGM (AS)
Opposite side
FOCUS LOCK LEVER
CONDENSER HEIGHT
ADJUSTMENT KNOB
FINE FOCUS KNOB
COARSE FOCUS KNOB
LED HOUSING

Page 8
8. Assembling
1. Insert the reected light illuminator ① on the frame and lock
the locking knob ② with the provided Allen wrench. (Fig. 1)
2. Connect the illuminator cable ③ to the socket ④placed on
the back side of the frame. (Fig. 2)
3. Insert the optical head above the epi-illuminator and tighten
the locking screw. (Fig. 3)
• Hold the head with one hand during the locking in order
to avoid that the head falls.
FFig. 1ig. 1
FFig. 2ig. 2
FFig. 4ig. 4
FFig. 3ig. 3
①
②
③
④
4. Insert both eyepieces into the tubes of the optical head. (Fig.
4)

Page 9
5. Insert the polarizer. (Fig. 5)
6. Remove the protection cap and insert analyzer. (Fig. 6-7)
7. Insert the power supply jack in the connector placed on the
back side of the frame. (Fig. 8)
FFig. 5ig. 5
FFig. 6ig. 6
FFig. 7ig. 7
FFig. 8ig. 8

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9. Summary of transmitted light observation procedures
(Used commands) (Chapter)
Main switch
Nosepiece
Coarse and fine focus knobs
Observation head
Diopter adjustment ring
Aperture diaphragm
Nosepiece
Brightness adjustment knob
Coarse and fine focus knobs
Put on “I” the main switch and adjust brightness
Insert 10x objective into the light path
Focus the specimen
Begin observation
Adjust aperture diaphragm
Insert the desired objective into the light path and focus the
specimen
Adjust interpupillary distance
Adjust diopter
Adjust brightness
10
7
7
7
7
10
10
10
10
Slide holder
X/Y movement knobs
Place a specimen on the stage 10
7
Brightness adjustment knob 10

Page 11
10. Use of the microscope in transmitted light
10.1 Turning on the instrument
Turn the main switch ①on the rear of the stand to “I”. (Fig. 9)
• There is a three-position switch on the rear of the stand: po-
sition “I” turns on the transmitted light, position “II” turns on
the reected light and position “O” turns o󰀨 the microscope.
10.2 Light intensity adjustment
Operate on the light intensity adjustment knob ② to increase /
decrease the illumination voltage. (Fig. 10)
10.3 Adjusting the interpupillary distance
Observing with both eyes, hold the two eyepiece prism assem-
blies. Rotate them around their common axis until the elds of
view coincide.
• The graduation on the interpupillary distance indicator
③, pointed by the spot “.” on the eyepiece holder, shows
thedistancebetweentheoperator’seyes.(Fig.11)
The range of the interpupillary distance is 48-75 mm.
10.4 Diopter adjustment
1. Look into the right eyepiece with your right eye only, and
focus on the specimen.
2. Look into the left eyepiece with your left eye only. If the
image is not sharp, use the diopter adjustment ring ④to
compensate. (Fig. 12)
• The adjustment range is ±5 diopter. The number indicated
on the adjustment ring graduation should correspond to
the operator’s diopter correction.
FFig. 9ig. 9
FFig. 10ig. 10
①
②
FFig. 12ig. 12
④
FFig. 11ig. 11
③

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10.5 Coarse focus tension adjustment
• Adjust the tension using the provided tool.
The tension of the coarse focusing knob is factory preset.
1. To modify the tension according to personal’s needs, rotate
the ring ① . (Fig. 13)
• Clockwise rotation increases the tension.
• If the tension is too loose, the stage could go lower by itself or
the focus easily lost after ne adjustment. In this case, rotate
the knob in order to increase the tension .
10.6 Coarse upper limit lever
The upper limit knob has two functions: prevent the contact
between slide and objective and acts as “focus memory”.
1. After focusing the specimen, pull the lever ③ toward the
front of the microscope and lock it. (Fig. 14)
• In this way the focus upper limit is set.
2. Now one can lower the stage with coarse focus knob, replace
the specimen and raise again the stage up to the upper limit:
specimen will be in approximate focus and will need a ne
adjustment to get the proper focus.
• Fine focus movement is not a󰀨ected by the coarse
focus lock.
• To unlock, move the lever in the opposite direction to the
one used for the locking.
10.7 Stage
Stage accepts metallurgical samples with thickness max 15 mm,
placed on a standard support 26 x 76 mm.
1. Open the spring arm of the slide holder ③ and place frontally
the slides on the stage. (Fig. 15)
2. Gently release the spring arm of the slide holder.
• A sudden release of the spring arm could cause the
falling of the slide.
10.7.1 Use of the glass plate
When using embedded metallographic specimens it may be
necessary to use the square glass plate.
1. Open the spring arm of the slide holder ③ and place frontally
the glass plate by aligning the positioning notch ④ so that
the movable arm ts directly into the notch. (Fig. 16)
2. Gently release the spring arm of the slide holder.
• The glass plate has a positioning notch to facilitate
insertion of the movable arm.
• This notch is provided and does not indicate a glass
breakage.
FFig. 13ig. 13
①
FFig. 16ig. 16
③
FFig. 14ig. 14
LOCK
UNLOCK
②
FFig. 15ig. 15
③
④

Page 13
10.8 Centering the condenser
• The condenser is mounted and pre-centered before ship-
ment from the factory.
• To remove the condenser, use a 1.5 mm Allen key and turn
the xing screw on the right-hand side of the condenser hold-
er.
If re-centering is necessary, proceed as follow:
1. Insert the 5x objective into the optical path (if the 5x objective
is not available, use the lower magnication objective).
2. Focus the specimen.
3. Close the aperture diaphragm using the ① dial, moving the
dial towards the “4” value for the 5X objective. (Fig. 17)
4. Raise the condenser to the end of its travel by turning the
condenser height adjustment screw ② on the left side of the
condenser holder.
5. Centre the condenser using the centering screws ③ until the
eld of view is evenly lit (no lighter or darker areas within the
eld of view).
6. Then open the diaphragm completely.
10.9 Aperture diaphragm
The Numerical Aperture (N.A.) value of the aperture diaphragm
a󰀨ects the image contrast. Increasing or reducing this value one
can vary resolution, contrast and depth of focus of the image.
1. Move the aperture ring ① (Fig. 18) to the value corresponding
to the objective in use. You can move the dial to lower or
higher values to adjust the observation to your preference.
• With low contrast specimens set the numerical aperture
value (printed on the condenser ring) to about 70%-80% of
the objective’s N.A. If necessary, remove on eyepiece and,
looking into empty sleeve, adjust the condenser’s ring in
order to obtain an image like the one in g. 19. FFig. 18ig. 18
①
FFig. 17ig. 17
③
①
②
30-20%
FFig. 19ig. 19
FIELD OF VIEW
APERTURE
DIAPHRAGM 70-80%

Page 14
11. Summary of reflected light observation procedures
(Used commands) (Chapter)
Nosepiece
Observation head
Diopter adjustment ring
Aperture diaphragm
Field diaprhagm
Nosepiece
Brightness adjustment knob
Coarse and fine focus knobs
Coarse and fine focus knobs
Put on “II” the main switch and adjust brightness
Insert 10x objective into the light path
Focus the specimen
Begin observation
Adjust aperture and field diaphragms
Insert the desired objective into the light path and focus the
specimen
Adjust interpupillary distance
Adjust diopter
Adjust brightness
7
7
7
7
10
10
12
12
10
Slide holder
X/Y movement knobs
Place a specimen on the stage 10
7
Remove polariser, analyser and filters
from optical path
Main switch 10
Brightness adjustment knob 10
Filter slider
Insert the desired colour filter 12

Page 15
12.1 Turning on the instrument
Turn the main switch ①on the rear of the stand to “II”. (Fig. 20)
12.2 Light intensity adjustment
Operate on the light intensity adjustment knob ② to increase /
decrease the illumination voltage. (Fig. 21)
12.3 Useofelddiaphragm(FS)
• Theeldandaperturediaphragmsontheilluminatorfor
reectedlightarepre-centeredbeforeshipmentfromthe
factory and do not require further centering.
1. Move the selector switch ③ on the reected light illuminator
toward left to position .(Fig. 22)
2. Place the sample on the stage, insert the 10x objective into
the optical path and focus.
3. In normal use, open the aperture until the image circumscribes
the eld of view.
12. Use of the microscope in reflected light
FFig. 20ig. 20
FFig. 21ig. 21
①
②
FFig. 22ig. 22
FFig. 23ig. 23
③
12.4 E󰀨ectsofelddiaphragm
The eld diaphragm adjusts the illuminated area to obtain an
image with high contrast. Adjust the eld diaphragm according
to the lens in use until the iris diaphragm circumscribes the eld
of view to eliminate unnecessary light to the eyepieces. (Fig. 23)

Page 16
12.5 Useofaperturediaphragm(AS)
The numerical aperture value (N.A.) of the aperture diaphragm
a󰀨ects image contrast. Increasing or decreasing this value ac-
cording to the numerical aperture of the lens changes the resolu-
tion, contrast and depth of eld of the image.
1. Move the aperture ring ④ (Fig. 24) to open or close the dia-
phragm to the desired position.
• For low-contrast specimens, set the numerical aperture
value to approximately 70%-80% of the objective’s N.A. If
necessary, remove an eyepiece and, looking into the empty
eyepiece holder, adjust the condenser ring until an image like
that in Fig. 19 is obtained.
12.6 Useofcolorlters
1. Insert the lter ① in one of the two slots ② placed in the
upper part of the reected light illuminator. (Fig. 25).
Filter Application
Blue Converts the source color temperature to
daylight
Green Increases the contrast in black and white
photography
Yellow Contrast lter for the observation of wafers
and semiconductors
Frosted white Reduces lighting irregularities by creating a
homogeneous eld of view
FFig. 24ig. 24
④
FFig. 26ig. 26
FFig. 27ig. 27
FFig. 25ig. 25
①
②
12.7 Use of polarized light
1. Insert the polarizer all the way into the optical path. (Fig. 26)
2. Insert the analyzer all the way into the optical path. (Fig. 27)

Page 17
3. Put a at mirror on the stage and get into approximate focus
the mirror surface.
4. While observing into the eyepieces, rotate the scale of the
analyzer ① until you achieve the darkest image. (Fig. 28)
• Now the light extinction (so-called “crossed Nicol position”)
is achieved.
5. Put a specimen on the stage, focus the specimen and begin
observation.
FFig. 28ig. 28
①

Page 18
13. Microphotography
13.1 Use of C-mount cameras
1. Loosen the clamping screw ① on the trinocular port and
remove the dust cap ②. (Fig. 29)
①
②
①
②
FFig. 35ig. 35
FFig. 36ig. 36
FFig. 37ig. 37
④
①
②
③
④
③
2. Screw the C-mount adapter ③to the camera ④ and insert
the round dovetail of the C-mount into the empty hole of the
trinocular port, then tighten the clamping screw ①. (Fig. 30)
13.2 Useofreexcameras
1. Insert the Reex adapter ① into the relay tube to the micro-
scope ②.
2. Screw the “T2” ring ③ (not provided) to the reex adapter.
3. Connect the Reex camera ④ to the “T2” ring just installed
(Fig. 31).
4. Mount the other end of the relay tube ② into the empty hole
of the trinocular port, then tighten the clamping screw. (Fig.
29)
• “T2” ring is not provided along with the microscope, but is
commercially available.
• While shooting dark specimens, darken eyepieces and view-
nder with a dark cloth to minimize the di󰀨used light.
• To calculate the magnication of the camera: objective magni-
cation * camera magnication * lens magnication.
• If using an SLR camera, mirror movement may cause the
camera to vibrate.
• Wesuggestliftingthemirror,usinglongexposuretimes
and a remote cord.

Page 19
14. Maintenance
Microscopy environment
This microscope is recommended to be used in a clean, dry and shock free environment with a temperature of 5°-40°C and
a maximum relative humidity of 85 % (non condensing). Use a dehumidier if needed.
To think about when and after using the microscope
• The microscope should always be kept vertically when moving it and be careful so that no moving
parts, such as the eyepieces, fall out.
• Never mishandle or impose unnecessary force on the microscope.
• Never attempt to service the microscope yourself.
• After use, turn o󰀨 the light immediately, cover the microscope with the provided dust-cover, and keep
it in a dry and clean place.
Electrical safety precautions
• Before plugging in the power supply, make sure that the supplying voltage of your region matches
with the operation voltage of the equipment and that the lamp switch is in o󰀨-position.
• Users should observe all safety regulations of the region. The equipment has acquired the CE safety
label. However, users do have full responsibility to use this equipment safely.
Cleaning the optics
• If the optical parts need to be cleaned try rst to: use compressed air.
• If that is not su󰀩cient: use a soft lint-free piece of cloth with water and a mild detergent.
• And as a nal option: use the piece of cloth moistened with a 3:7 mixture of ethanol and ether.
• Note:ethanolandetherarehighlyammableliquids.Donotusethemnearaheatsource,nearsparksornear
electric equipment. Use these chemicals in a well ventilated room.
• Remember to never wipe the surface of any optical items with your hands. Fingerprints can damage the optics.
• Do not disassemble objectives or eyepieces in attempt to clean them.
Forthebestresults,usetheOPTIKAcleaningkit(seecatalogue).
If you need to send the microscope to Optika for maintenance, please use the original packaging.

Page 20
15. Troubleshooting
Review the information in the table below to troubleshoot operating problems.
PROBLEM CAUSE SOLUTION
I. Optical Section:
LED operates, but eld of view remains
dark. Power supply is unplugged. Connect
Brightness is too low Set brightness to a proper level
Color filter or oblique illumination slider
are in a wrong position Move to a click stop
Field of view is obscured or not evenly
illuminated Revolving nosepiece is not correctly engaged. Make sure that the revolving nose- piece clicks properly into place.
Oblique illumination slider is in a wrong
position Move the slider to a fully open position
Dirt or dust is visible in the field of view. Dirt/dust on the specimen Clean the specimen
Dirt/dust on the eyepieces Clean the eyepieces
Image looks double Aperture iris diaphragm is stopped
down too far. Open aperture iris diaphragm.
The field diaphragm (both for reflected
light and transmitted light) is not well
centered.
Set the diaphragm according to Koe-
hler settings.
Visibility is poor.
• Image is not good;
• Contrast is poor;
• Details are indistinct;
• Image glares
Revolving nosepiece is in an incorrect
position Move the nosepiece to a click stop
Aperture iris diaphragm is too closed
or too open. Adjust aperture iris diaphragm.
Dust or dirt on lenses (condenser, ob-
jectives, eyepieces and slide) Clean thoroughly.
For reected light observation, the
specimen must not have any cover-
glass
Use specimens without coverglass
Focus is not even Slide holder is not at. Move the spec-
imen to a at position.
One side of the image is unfocused Revolving nosepiece is in an incorrect
position Move the nosepiece to a click stop
Slide is mounted not in a at position
(tilted) Place the specimen in a at position on the stage
II. Mechanical Section:
Coarse focus knob is hard to turn Tension adjustment ring is too tight Loosen tension adjustment ring
Focus is unstable Tension adjustment ring is too loose Tighten tension adjustment ring
III. Electrical Section
LED doesn’t turn on. Power supply not connected Check for proper connection
Brightness is not enough Brightness setting is too low Adjust brightness
Light blinks Power supply not well connected Check for proper connection
IV. Observation tube
Field of view of one eye does not match
that of the other. Interpupillary distance is incorrect. Adjust interpupillary distance.
Incorrect diopter adjustment. Adjust diopter.
Your view is not accustomed to micro-
scope observation. Upon looking into eyepieces, try look- ing at overall field before concentrating
on specimen range. You may also find
it helpful to look up and into distance
for a moment before looking back into
microscope.