ThermoFisher Scientific thermo scientific Heraeus Multifuge X1R User manual

Thermo Scientific
Heraeus Multifuge X1R Centrifuge
Instruction Manual
50156623-b • 03 / 2020
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Thermo Scientific Heraeus Multifuge X1R i
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Chapter 1
Chapter 2
Chapter 3
Chapter 4
Preface ....................................................................................................................................... iii
Scope of supply ........................................................................................................................ iii
Intended use ..............................................................................................................................iv
Accident prevention .................................................................................................................iv
Precautions .................................................................................................................................v
Introduction and description ............................................................................................... 1-1
Characteristics of the Thermo Scientific Heraeus Multifuge X1R
............................. 1-2
Technical data ......................................................................................................... 1-3
Directives, standards and guidelines ......................................................................................... 1-4
Functions and features .............................................................................................................. 1-4
Mains supply ........................................................................................................... 1-4
Rotor selection ......................................................................................................... 1-5
Before use ............................................................................................................................... 2-1
Before setting up ...................................................................................................... 2-2
Transporting the centrifuge ..................................................................................... 2-2
Location .................................................................................................................. 2-2
Aligning the centrifuge ............................................................................................ 2-3
Mains Connection ................................................................................................... 2-3
Storage ..................................................................................................................... 2-4
Control panel .......................................................................................................................... 3-1
Control panel .......................................................................................................... 3-2
Keys ......................................................................................................................... 3-2
Operation ................................................................................................................................. 4-1
Switch on centrifuge ................................................................................................ 4-2
Door opening .......................................................................................................... 4-2
Close door ............................................................................................................... 4-2
Rotor installation ..................................................................................................... 4-2
Entering parameters ................................................................................................. 4-4
Centrifugation ....................................................................................................... 4-10
Temperature adaptation during standstill .............................................................. 4-11
Short-term centrifugation ...................................................................................... 4-11
Removing the rotor ................................................................................................ 4-11
Aerosol-tight rotors ................................................................................................ 4-12
Turning off the centrifuge ..................................................................................... 4-12
Table of Contents

Contents
ii Heraeus Multifuge X1R Thermo Scientific
System menu ..........................................................................................................Chapter 5 ................. 5-1
Description .............................................................................................................. 5-2
Maintenance and care .................................................................................................Chapter 6 ......... 6-1
................................................................................................................................ 6-2
Cleaning intervals .................................................................................................... 6-2
Cleaning .................................................................................................................. 6-2
Disinfection ............................................................................................................. 6-4
Decontamination ..................................................................................................... 6-5
Autoclaving .............................................................................................................. 6-5
Service of Thermo Fisher Scientific .......................................................................... 6-6
Troubleshooting ......................................................................................................Chapter 7 ............... 7-1
Mechanical emergency lid release ............................................................................. 7-2
Troubleshooting by user .......................................................................................... 7-3
When to contact customer service ............................................................................ 7-6
Chemical compatibility chart ...............................................................................A-1
Contact information ................................................................................................B-1

Thermo Scientific Heraeus Multifuge X1R iii
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Preface
Before starting to use the centrifuge, read through this instruction manual carefully and follow the
instructions.
The information contained in this instruction manual is the property of Thermo Fisher Scientific; it is
forbidden to copy or pass on this information without explicit approval.
Failure to follow the instructions and safety information in this instruction manual will result in the
expiration of the sellers warranty.
Scope of supply
If any parts are missing, please contact your nearest Thermo Fisher Scientific representative.
Article number Quantity Check
Heraeus Multifuge X1R Centrifuge 1
Power supply cable 1
Instruction manual
50156623 1
This symbol refers to general hazards.
CAUTION means that material damage could occur.
WARNING means that injuries or material damage or contamination could occur.
This symbol refers to biological hazards.
Observe the information contained in the instruction manual to keep yourself and your
environment safe.

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iv Heraeus Multifuge X1R Thermo Scientific
Intended use
•This centrifuge is designed to separate sample mixtures of different densities like chemicals,
environmental samples and other non-human body samples.
Accident prevention
WARNING Plug the centrifuge only into sockets which have been properly grounded.
WARNING If a hazardous situation occurs, turn off the power supply to the centrifuge
and leave the area immediately.
Prerequisite for the safe operation of the Heraeus Multifuge X1R is a work environment
incompliance with standards, directives and trade association safety regulations and
properinstruction of the user.
The safety regulations contain the following basic recommendations:
•Maintain a radius of at least 30 cm around the centrifuge.
•Implementation of special measures which ensure that no one can approach the
centrifuge for longer than absolutely necessary while it is running.
The mains plug must be freely accessible at all times. Pull out the power supply plug or
disconnect the power supply in an emergency.

Thermo Scientific Heraeus Multifuge X1R v
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Precautions
In order to ensure safe operation of the Heraeus Multifuge X1R, the following general
safety regulations must be followed:
•The centrifuge should be operated by trained specialists only.
•The centrifuge is to be used for its intended use only.
•Do not move the centrifuge while it is running.
•Do not lean on the centrifuge.
•Use only rotors and accessories for this centrifuge which have been approved by Thermo
Fisher Scientific. Exceptions to this rule are commercially available glass or plastic
centrifuge tubes, provided they have been approved for the speed or the RCF value of
the rotor.
•Do not use rotors which show any signs of corrosion and/or cracks.
•Do not touch the mechanical components of the rotor and do not make any changes to
the mechanical components.
•Use only with rotors which have been properly installed. Follow the instructions on the
Auto-Lock in section “Rotor installation” on page 4-2.
•Use only with rotors which have been loaded properly. Follow the instructions given in
the rotor manual.
•Never overload the rotor. Follow the instructions given in the rotor manual.
•Never start the centrifuge when the lid is open.
•Never open the lid until the rotor has come to a complete stop and this has been
confirmed in the display.
•The lid emergency release may be used in emergencies only to recover the samples from
the centrifuge, e.g. during a power failure (see section “Mechanical emergency lid
release” on page 7-2).
•Never use the centrifuge if parts of its cover panels are damaged or missing.
•Do not touch the electronic components of the centrifuge or alter any electronic or
mechanical components.
•Please observe the safety instructions.
Please pay particular attention to the following aspects:
•Location: well-ventilated environment, set-up on a level and rigid surface with adequate
load-bearing capacity.
•Rotor installation: Make sure the rotor is locked properly into place before operating the
centrifuge.

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vi Heraeus Multifuge X1R Thermo Scientific
•Especially when working with corrosive samples (salt solutions, acids, bases), the
accessory parts and vessel have to be cleaned carefully.
•Always balance the samples.
Centrifuging hazardous substances:
•Do not centrifuge explosive or flammable materials or substances which could react
violently with one another.
•The centrifuge is neither inert nor protected against explosion. Never use the centrifuge
in an explosion-prone environment.
•Do not centrifuge inflammable substances.
•Do not centrifuge toxic or radioactive materials or any pathogenic micro-organisms
without suitable safety precautions.
When centrifuging microbiological samples from the Risk Group II (according to the
Bio-safety Manual" of the World Health Organization (WHO)), aerosol-tight biological
seals have to be used.
For materials in a higher risk group, extra safety measures have to be taken.
•If toxins or pathogenic substances have gotten into the centrifuge or its parts,
appropriate disinfection measures have to be taken (see “Disinfection” on page 6-4).
•Highly corrosive substances which can cause material damage and impair the
mechanical stability of the rotor, should only be centrifuged in corresponding protective
tubes.
Remaining risk: Improper use can cause damages, contamination, and injuries
with fatal consequences.
Remaining risk: Improper use can cause damages, contamination, and injuries
with fatal consequences.

Thermo Scientific Heraeus Multifuge X1R 1-1
1
Introduction and description
Contents
•“Characteristics of the Thermo Scientific Heraeus Multifuge X1R” on page 1-2
•“Technical data” on page 1-3
•“Directives, standards and guidelines” on page 1-4
•“Functions and features” on page 1-5
•“Mains supply” on page 1-5
•“Rotor selection” on page 1-6

1-2 Heraeus Multifuge X1R Thermo Scientific
1Introduction and description
Characteristics of the Thermo Scientific Heraeus Multifuge X1R
Characteristics of the Thermo Scientific Heraeus Multifuge X1R
This centrifuge is designed to separate sample mixtures using different rotors according to application
needs.
The set speed is reached within seconds. The maintenance-free induction motor ensures quiet and
low-vibration operation even at high speeds, and guarantees a very long lifetime.
The user-friendly control panel makes it easy to pre-set the speed, RCF value, run time, temperature,
and run profile (acceleration and braking curves). You can choose between the display of speed and
RCF or the entry mode.
These settings can be changed even while the centrifuge is running.
With the help of the key , you can also centrifuge a sample for just a few seconds, if called for.
The Heraeus Multifuge X1R is equipped with various safety features:
•The housing and rotor chamber consist of steel plate, the interior of armour steel, while the front
panel is made of high-impact resistant plastic.
•The lid is equipped with a view port and a lock.
•The lid of the centrifuge can only be opened while the centrifuge is switched on and the rotor has
come to a complete stop. The centrifuge cannot be started until the lid has been closed properly.
•The integrated rotor detection systems ensures that no inadmissible speed settings can be
preselected.
•Electronic imbalance recognition
•Lid emergency release: For emergencies only, e.g. during power failures (see “Mechanical
emergency lid release” on page 7-2)

Thermo Scientific Heraeus Multifuge X1R 1-3
1Introduction and description
Technical data
Technical data
The technical data of the Heraeus Multifuge X1R is listed in the following table.
Table 1-1. Technical Data Heraeus Multifuge X1R
Feature
Environmental conditions
Heraeus Multifuge X1R
-Use in interior spaces
-Altitudes of up to 2,000m above sea level
-max. relative humidity 80% up to 31 °C; decreasing linearly up to 50%
relative humidity at 40 °C.
Permissible ambient temperature +2 °C to +35 °C
Overvoltage category II
Pollution degree 2
Voltage 230 V
Heat dissipation 4778 BTU/h
IP 20
Run time unlimited
Max speed nmax 15200 rpm (depending on the rotor)
Min speed nmin 300 rpm
Maximum RCF value at nmax 25830 x g
Maximum kinetic energy 62.5 kJ
Noise level at maximum speed < 63 dB (A)
Temperature setting range -10 °C to +40 °C
Dimensions refrigerated
Height
Height with lid open
Width
Depth
Table top height
360 mm
870 mm
623 mm
605 mm
310 mm
Weight without rotor 91.5 kg
Refrigerant Quantity Pressure GWP CO2e Order No.
75004249 R-404A 0.38 kg 31 bar 3922 1.49 t
Centrifuge
Thermo Scientific
Heraeus Multifuge X1R
Contains fluorinated greenhouse gases in a hermetically sealed system.
Refrigerant

1Introduction and description
Directives, standards and guidelines
1-4 Heraeus Multifuge X1R Thermo Scientific
Directives, standards and guidelines
Table 1-2. Directives, standards and guidelines
Tension /
frequency
220-230 V, 50 / 60 Hz
Standards
IEC 61010-1
IEC 61010-2-020
EN 61326-1 Class B
EN ISO 9001
Mains supply
The following table contains an overview of the electrical connection data for the Heraeus Multifuge
X1R. This data is to be taken into consideration when selecting the mains connection socket.
Functions and features
The following table gives an overview of the important functional and performance characteristics
of the Heraeus Multifuge X1R.
Table 1-3. Functions and Features
Component / function Description / features
Galvanized steel chassis with armoured platingStructure / Housing
Stainless steelChamber
Induction drive without carbon brushesDrive
Easy-to-clean keypad and display surfaceKeys and display
Controls Microprocessor-controlled
Internal memory The most recent data is saved
Functions RCF-selection, temperature control, pretemp with cooling equipped
devices
9 acceleration and 10 braking curvesAcceleration / braking profiles
Rotor recognition Automatic
Imbalance recognition Electronic, contingent on rotor and speed
Lid lock Automatic lid closing and locking starting from an initial hold position
Table 1-4. Electrical connection data of the Heraeus Multifuge X1R
Cat. Mains
voltage Frequency Rated current Power
consumption
Equipment
fuse Building fuse
15 AT1400 W 16 AT
Refrigerated 230 V ±10% 50 /60 Hz 8 A
1 Please contact the Thermo Fisher service to run the unit at 60 Hz Linefrequency.

Thermo Scientific Heraeus Multifuge X1R 1-5
1Introduction and description
Rotor selection
Rotor selection
The Heraeus Multifuge X1R of the is supplied without arotor.
Various Thermo Scientific rotors are available to choose from.
The technical data of the rotors and the corresponding adapters and reduction sleeves for various
commercially available containers can be found in the corresponding rotor operating manuals.
For more information visit our website at: http://www.thermofisher.com/centrifuge
TX-400 75003181
with round buckets 75003655
TX-200 75003658
with round buckets 75003659
BIOShieldTM 720 75003183
M-20 75003624
Microliter 30 x 2 75003652
CLINIConicTM 75003623
8 x 50 sealed 75003694
Fiberlite F15-6 x 100y75003698
Fiberlite F13-14 x 50cy75003661
Fiberlite F15-8 x 50cy75003663
Fiberlite F21-48 x 275003664
Microliter 48 x 2 75003602
HIGHConicTM II 75003620
MicroClick 30 x 2 75005719

Thermo Scientific Heraeus Multifuge X1R 2-1
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Before use
Contents
•“Before setting up” on page 2-2
•“Transporting the centrifuge” on page 2-2
•“Location” on page 2-2
•“Aligning the centrifuge” on page 2-3
•“Mains Connection” on page 2-3
•“Storage” on page 2-4

2Before use
Before setting up
2-2 Heraeus Multifuge X1R Thermo Scientific
Before setting up
1. Check the centrifuge and the packaging for any shipping damage.
Inform the shipping company and Thermo Fisher Scientific immediately if any damage is
discovered.
2. Remove the packaging.
3. Check the order for completeness (see “Scope of supply” on page iii).
If the order is incomplete, please contact Thermo Fisher Scientific.
Transporting the centrifuge
•Due to its weight (see “Technical data” on page 1-3), the centrifuge should be carried by several
people.
•Always lift the centrifuge at both sides.
Figure 2-1. Lifting the centrifuge at both sides.
•The centrifuge can be damaged by impacts.
•T
ransport the centrifuge upright and if at all possible in its packaging.
Location
The centrifuge should only be operated indoors.
The set-up location must fullfil the following requirements:
•A safety zone of at least 30 cm must be maintained around the centrifuge.
People and hazardous substances must be kept out of the safety zone while centrifuging.
•The supporting structure must be stable and free of resonance, for example a level laboratory
bench.
•The supporting structure must be suitable for horizontal setup of the centrifuge.
WARNING Always lift the centrifuge on both sides. Never lift the centrifuge by its front
or the back panel.
Always remove the rotor before moving the centrifuge.

2 Before use
Aligning the centrifuge
Thermo Scientific Heraeus Multifuge X1R 2-3
•The centrifuge should not be exposed to heat and strong sunlight.
•The set-up location must be well-ventilated at all times.
Aligning the centrifuge
The horizontal alignment of the centrifuge must be checked every time after moving it to a different
location.
The supporting structure must be suitable for horizontal setup of the centrifuge.
Mains Connection
Figure 2-2. Mains connection
1. Turn off the power supply switch on the back (press "0").
2. Plug the centrifuge into grounded electrical sockets only.
3. Check whether the cable complies with the safety standards of your country.
4. Make sure that the voltage and frequency correspond to the figures on the rating plate.
5. Establish the connection to the power supply with the connecting cable.
WARNING UV rays reduce the stability of plastics.
Do not subject the centrifuge, rotors and plastic accessories to direct sunlight.
CAUTION If the centrifuge isn’t level, imbalances can occur and the centrifuge can be
damaged.
Do not place anything under the feet to level the centrifuge.
Main power switch
Mains plug

2Before use
Storage
2-4 Heraeus Multifuge X1R Thermo Scientific
Storage
•Before storing the centrifuge and the accessories it must be cleaned and if necessary disinfected and
decontaminated.
•Store the centrifuge in a clean, dust-free location.
•Be sure to place the centrifuge on its feet.
•Avoid direct sunlight.

Thermo Scientific Heraeus Multifuge X1R 3-1
3
Control panel
Contents
•“Control panel” on page 3-2
•“Keys” on page 3-2

3Control panel
Control panel
3-2 Heraeus Multifuge X1R Thermo Scientific
Control panel
The control panel contains the keys and displays of the centrifuge (only the power switch is located on
the back of the device). All parameters can be selected and changed during operation.
Keys
The keys allow user intervention for controlling the operating mode as follows:
PULSE
Program selection keys OPEN
Display
Directory key SPEED START
TIME ENTER
Braking and acceleration key
TEMP
Changing the display mode
numeric pad
Bucket
STOP
Changing the display mode
Changing the display mode
Speed / RCF Value
Run time / Remaining run time
chamber / sample temperature
Key Display contents
Start
Normal start of the centrifuge
Stop
End run manually
Open lid Automatic release (possible only when device is switched on); Emergency
release (see “Mechanical emergency lid release” on page 7-2)
Pulse
By pressing the key the centrifuge starts immediately and accelerates
up to the end speed. Releasing the key initiates a stopping process at the
highest braking curves.
Directory
By pressing the key up and down you change the value in thedisplay.
Bucket
Use the key in order to have all available bucket types displayed in
succession.
Braking and accelera-
tion curves
Use the key to toggle between braking and acceleration curves.
Changing the display
mode
Use the key to change the display mode. (speed / RCF value, sample /
chamber temperature, run time counter from start or preset speed on)

Thermo Scientific Heraeus Multifuge X1R 4-1
4
Operation
Contents
•“Switch on centrifuge” on page 4-2
•“Door opening” on page 4-2
•“Rotor installation” on page 4-2
•“Entering parameters” on page 4-4
•“Centrifugation” on page 4-10
•“Temperature adaptation during standstill” on page 4-11
•“Short-term centrifugation” on page 4-11
•“Removing the rotor” on page 4-11
•“Turning off the centrifuge” on page 4-12