Teledyne UA-6 Operating instructions

UA-6
Absorbance Detector
Installation and Operation Guide
Part #69-0943-266 of Assembly 60-0943-261
Copyright © 1991. All rights reserved, Teledyne Isco
Revision M, October 2013

Foreword
This instruction manual is designed to help you gain a thorough understanding of the operation of
the equipment. Teledyne Isco recommends that you read this manual completely before placing the
equipment in service.
Although Teledyne Isco designs reliability into all equipment, there is always the possibility of a
malfunction. This manual may help in diagnosing and repairing the malfunction.
If a problem persists, call or e-mail the Teledyne Isco Technical Service Department for assistance.
Simple difficulties can often be diagnosed over the phone.
If it is necessary to return the equipment to the factory for service, please follow the shipping
instructions provided by the Customer Service Department, including the use of the Return
Authorization Number specified. Be sure to include a note describing the malfunction. This
will aid in the prompt repair and return of the equipment.
Teledyne Isco welcomes suggestions that would improve the information presented in this manual
or enhance the operation of the equipment itself.
Teledyne Isco is continually improving its products and reserves the right to change product
specifications, replacement parts, schematics, and instructions without notice.
Contact Information
Customer Service
Phone: [phone] (USA, Canada, Mexico)
[phone] (Outside North America)
Fax: [phone]
Email: [email protected]
Technical Support
Phone: Toll Free [phone] (Samplers and Flow Meters)
Toll Free [phone] (Syringe Pumps and Liquid Chromatography)
Email: [email protected]
Return equipment to: 4700 Superior Street, Lincoln, NE 68504-1398
Other Correspondence
Mail to: P.O. Box 82531, Lincoln, NE 68501-2531
Email: [email protected]
Revised September 2012

v
Warnings and Cautions
Symboles de Sécurité
The lightning flash and arrowhead within the triangle is a warning
sign alerting you to “dangerous voltage” inside the product.
The exclamation point within the triangle is a warning sign alerting
you to important instructions in this manual.
Ce symbole signale la présence d’un danger d’électrocution.
Ce symbole signale l’existence d’instructions importantes
relatives au produit dans ce manuel.

vi
Warnungen und Vorsichtshinweise
Advertencias y Precauciones
Esta señal alerta sobre la presencia de alto voltaje en el interior
del producto.
Der gepfeilte Blitz im Dreieck ist ein Warnzeichen, das Sie vor
„
gefährlichen Spannungen
“
im Inneren des Produkts warnt.
Das Ausrufezeichen in Dreieck ist ein Warnzeichen, das Sie
darauf aufmerksam macht, daß wichtige Anleitungen zu diesem
Handbuch gehören.
Esta señal le advierte sobre la importancia de las instrucciones
del manual que acompañan a este producto.

vii
CAUTION:
LIQUIDS ASSOCIATED WITH THIS INSTRUMENT MAY
BE CLASSIFIED AS CARCINOGENIC, BIOHAZARD,
FLAMMABLE, OR RADIOACTIVE. SHOULD THESE LIQ-
UIDS BE USED, IT IS HIGHLY RECOMMENDED THAT
THIS APPLICATION BE ACCOMPLISHED IN AN ISO-
LATED ENVIRONMENT DESIGNED FOR THESE TYPES
OF MATERIALS IN ACCORDANCE WITH FEDERAL,
STATE, AND LOCAL REGULATORY LAWS, AND IN
COMPLIANCE WITH YOUR COMPANY’S CHEMICAL/
HYGIENE PLAN IN THE EVENT OF A SPILL.
IN ALL CASES, WHEN USING TELEDYNE ISCO INC. INSTRU-
MENTATION, PRUDENCE AND COMMON SENSE MUST BE
USED.
CAUTION: If this instrument is used in a manner not
specified in this manual, the protection provided by the
equipment may be impaired.

viii
AVIS:
LES LIQUIDES QUI SONT ANALYSÉS DANS CET INSTRU-
MENT PEUVENT ÊTRE CANCÉRIGÈNES, HASARDS
BIOLOGIQUES, INFLAMMABLES, OU RADIOACTIFS.
SI VOUS DEVEZ UTILISER CES LIQUIDES HASARDEUX, IL
EST TRÈS RECOMMANDÉ QUE VOUS LE FAITES À
L'INTÉRIEUR D'UN ENVIRONNEMENT ISOLÉ CONÇU
POUR TELS LIQUIDES.
CET ENVIRONNEMENT ISOLÉ DEVRAIT ÊTRE CONSTRUIT
SELON LES RÈGLEMENTS FÉDÉRAUX, PROVINCIAUX, ET
LOCAUX, AUSSI QUE LE PLAN DE VOTRE COMPAGNIE
QUI CONCERNE L'ÉVÈNEMENT D'UN ACCIDENT AVEC
LES MATIÈRES HASARDEUSES.
EN TOUT CAS, UTILISEZ TOUJOURS L'INSTRUMENTATION
D'ISCO AVEC PRUDENCE ET SENS COMMUN.
AVIS: Si vous employez cet instrument d’une manière
autre que celles qui sont specifiées dans ce manuel, la
protection fournie par l’instrument peut être affaiblie.
Cela augmentera votre risque de blessure.

vii
ADDENDUM
The chart recorder of the UA-6 can be set to produce charts based on cm/ml length of chart per unit vol-
ume pumped instead of the traditional cm/hr (length of chart per unit time).
For volume based charts, plug the square 4-pin connector on the cable (part number 60-1020-163 or
60-1020-228) into the socket labeled REMOTE CHART DRIVE on the rear panel of the UA-6.
The chart length per unit volume (cm/ml) is shown on the table below for each chart-speed setting on the
UA-6, the tubing size in the TRIS pump, and line frequency (60 Hz in the USA, 50 Hz in Europe, etc.).
The actual chart speed (cm/hr) when generating charts can be calculated from the following equation:
(Tris speed) x (Tris range) x (UA-6 chart speed range) x 0.049804867 (UA-6 line frequency) = chart speed
Example: 100% X 10.300 cm/hr, 60 Hz
100 X 10 X 1 X 300 X 0.049804867 60 = 249.02343 cm/hr
Example: 25% X 1, 15 cm/hr, 50 Hz
25 X 1 X 1 X 15 X 0.049804867 50 = 0.3735153 cm/hr.
CHART LENGTH PER UNIT VOLUME PUMPED (Approximate cm/ml)
FOR SINGLE CHANNEL OF PUMP
Chart Speed
cm/hr 60 Hz Line Frequency 50 Hz Line Frequency
1/16” tubing 1/8” tubing 1/16” tubing 1/8” tubing
300 1.70.52.00.6
150 0.83 0.25 1.0 0.3
60 0.33 0.1 0.4 0.12
30 0.17 0.05 0.2 0.06
15 0.083 0.025 0.1 0.03
60.033 0.01 0.04 0.012
30.017 0.005 0.02 0.006
1.5 0.0083 0.0025 0.01 0.003
0.6 0.0033 0.001 0.004 0.0012
0.3 0.0017 0.0005 0.002 0.0006
Note
Changing the jumper in the Tris pump from the default setting
(P104 pin 4-8) to P104 pin 3-7 will double the cm/ml. Refer to
Section 5 of the Tris manual for top removal and the circuit
board location of P104.
0 to 100% 50 or 60 Hz1 or 10

viii
TELEDYNE ISCO RESEARCH AND APPLICATION LABORATORY
The staff of Teledyne Isco’s Research and Applications Laboratory is available to assist
scientists in realizing and utilizing the full research potential of Teledyne Isco Instru-
ments. When an application problem arises, contact the laboratory director for assis-
tance. Our staff will do its utmost in analyzing and solving any problems. If you have
developed a unique application for a Teledyne Isco Instrument or are considering a new
use, we would be interested in learning about it.
TRADEMARKS
® Amphenol is a Registered Trademark of BTC Electronic Components
® Halar is a Registered Trademark of Allied Corporation
® Hastelloy C is a Registered Trademark of Cabot Corporation
® Chemraz is a Registered Trademark of Venitron Corporation
® Locktite is a Registered Trademark of Loctite Corporation

ix
COMMONLY ORDERED REPLACEMENT PARTS FOR THE TELEDYNE ISCO UA-6
These replacement parts are often ordered by UA-6 owners. All parts are pictured and
identified by number and description in Appendix A Replacement Parts in the back of this
manual.
* Replacement lamps are tested to meet specifications. Bubbles, mer-
cury spots, etc. within the quartz envelope are not detrimental to the
function of the lamp.
Description Part Number
UV lamp (Hg vapor) for Type 11 optical unit 60-0947-027*
chart paper 69-0943-193
pens 68-0947-020
flow cell windows: prep cell (10 mm diameter) 60-0644-052
Type 11 filters: 254 nm 60-1144-001
280 nm 60-1144-002
Type 11 source screens: 254 nm 60-1144-065
280 nm 60-1144-008
flow cell connectors 68-1844-173
right lamp reflector 60-1143-049
left lamp reflector 60-1143-050
flow cell ferrules for 1/16” tubing 68-0084-014

x

xi
Isco UA-6
Table of Contents
Section 1 Introduction
1.1 Optical Units for the UA-6 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1-1
1.1.1 Type 11 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1-1
1.2 Applications for the UA-6 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1-2
1.2.1 Liquid Chromatography . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1-2
1.2.2 Cuvette Reading . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1-2
1.3 Technical Specifications . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1-2
1.4 Controls and Indicators . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1-2
Section 2 Preparation for Use
2.1 Unpacking . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-1
2.2 Preparation for Use (Power) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-1
2.3 Installing the Chart Paper and Pen. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-1
2.4 Attachment of the Optical Unit to the UA-6 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-3
2.5 Changing Wavelengths with the Type 11 Optical Unit . . . . . . . . . . . . . . . . . . . . . 2-4
2.6 Installation of Flow Cells . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-5
2.7 Calibrating to an External Standard . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-6
2.8 Baseline Adjustment for Absorbance . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-6
Section 3 Operation
3.1 Introduction. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-1
3.2 Flow Cell Applications . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-2
3.2.1 Preparative Flow Cells (68-0080-001, 002, 034) . . . . . . . . . . . . . . . . . . . . . 3-2
3.2.2 High Flow Rate Cell (68-0080-023) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-2
3.3 Flow Cell Descriptions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-2
3.3.1 Preparative Flow Cells . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-3
3.3.2 High Flow Rate Cell . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-5
3.4 Flow/Pressure Characteristics . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-6
3.5 Tubing Connections for Plastic Lead Connectors . . . . . . . . . . . . . . . . . . . . . . . . . . 3-6
3.6 Tubing Connections for High Flow Rate Cell . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-7
3.7 Selecting Chromatography
Flow Cells . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-8
3.7.1 Pathlength . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-8
3.7.2 Flow Rate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-10
3.7.3 Preparative Cells . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-10
3.8 Avoiding Bubble Formation and Retention . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-10
3.9 Noise Filter . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-11
3.10 Built-in Chart Recorder . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-12
3.11 Built-In Peak Separator . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-12
3.11.1 PEAK SEPARATOR Switch . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-12
3.11.2 The Indicator LED . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-13
3.12 Performance and Use . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-13
3.12.1 Minimizing Trace Noise and Drift . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-13
3.12.2 Solvent Absorbance . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-16
3.13 Connection to Other Instruments . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-16
3.13.1 Foxy R1 and R2 Fraction Collectors . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-17

Isco UA-6
Table of Contents
xii
3.13.2 Teledyne Isco
Retriever 500 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-18
3.14 Remote Chart Drive Control by Non-Isco Equipment . . . . . . . . . . . . . . . . . . . . 3-18
3.15 Converting the Internal Recorder for Stand-Alone Operation . . . . . . . . . . . . . . 3-19
Section 4 Theory of Operation
4.1 General Description . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4-1
4.1.1 Optical Unit . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4-1
4.1.2 UA-6 Detector . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4-2
4.2 Detailed Description . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4-3
4.2.1 Auto Baseline Circuit . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4-3
4.2.2 Logarithmic Amplifier . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4-4
4.2.3 Gain Amplifiers . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4-4
4.2.4 Noise Filtering . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4-4
4.2.5 Recorder Offset/Set Lamp and Optics . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4-5
4.2.6 Peak Separation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4-5
4.2.7 Injection and Event Mark . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4-5
4.2.8 Lamp Brightness Regulator . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4-5
4.2.9 Power Supply . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4-6
4.2.10 Built-In Chart Recorder . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4-7
4.2.11 Pen Drive . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4-7
4.3 Type 11 Optical Unit Theory of Operation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4-8
4.3.1 General . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4-8
4.3.2 Absorbance Detector Circuit . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4-10
Section 5 Maintenance and Adjustment
5.1 Removal and Installation of the Case Top. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5-1
5.2 Part Replacement . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5-1
5.2.1 Recorder Replacement . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5-1
5.2.2 Recorder Drive String Installation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5-3
5.3 Timing Belt Tension Adjustment . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5-4
5.4 Cleaning the Enclosures . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5-5
5.5 Troubleshooting. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5-5
5.6 Type 11 Optical Unit Maintenance . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5-11
5.6.1 Type 11 UV Lamp Replacement . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5-11
5.6.2 Regulating Photocell Assembly Replacement . . . . . . . . . . . . . . . . . . . . . . 5-13
5.6.3 Filter Assembly Inspection . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5-15
5.6.4 Troubleshooting . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5-15
5.7 Flow Cell Maintenance and Adjustment . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5-18
5.7.1 Window or O-Ring Replacement . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5-18
5.7.2 Correcting for Flow Cell Window Leakage . . . . . . . . . . . . . . . . . . . . . . . . . 5-19
5.7.3 Troubleshooting . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5-20
5.7.4 Flow Cell Cleaning Procedures . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5-20
5.7.5 Cleaning the Back Pressure Regulator . . . . . . . . . . . . . . . . . . . . . . . . . . . 5-21
5.7.6 Service Department . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5-21
5.7.7 How to Ship Returns . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5-21
5.7.8 Purchasing Parts . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5-22
Appendix A Replacement Parts
A.1 Replacement Parts . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . A-1
List of Figures
1-1 UA-6 Detector with Type 11 Optical Unit . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1-2

Isco UA-6
Table of Contents
xiii
1-2 UA-6 Front Panel . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1-5
1-3 UA-6 Rear Panel . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1-6
1-4 Type 11 Controls and Connectors . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1-8
2-1 Recorder Chart Paper and Pen Installation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-2
2-2 Attaching an Optical Unit to the UA-6 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-3
2-3 Changing Wavelengths in the Type 11 Optical Unit . . . . . . . . . . . . . . . . . . . . . . . 2-4
2-4 Changing the Source Screen in the Type 11 Optical Unit . . . . . . . . . . . . . . . . . . . 2-5
2-5 Installing Flow Cells . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2-6
3-1 Preparative Flow Cell . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-3
3-2 Flow Cell Cross-Section . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-4
3-3 Flow Cell Flow/Pressure Characteristics . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-5
3-4 Medium Pressure (400 psi, 28 kg/cm²) Lead Connector Installation . . . . . . . . . . 3-7
3-5 Lead Connector Assembly for High flow Rate Flow Cell . . . . . . . . . . . . . . . . . . . . 3-8
3-6 Ferrule Application Information . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-9
3-7 Effect of Noise Filter on Baseline Noise . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-11
3-8 Sample Chromatogram . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-12
3-9 Absorbance Curves of Four Commonly Used Solvents . . . . . . . . . . . . . . . . . . . . 3-15
3-10 Input and Output Signals of the UA-6 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-16
3-11 Cable Connection to the Foxy R1 or R2 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-17
3-12 Cable connection to the Retriever 500 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-18
3-13 Suggested Circuit for a Remote Chart Drive . . . . . . . . . . . . . . . . . . . . . . . . . . 3-19
3-14 Problem Analysis - Drift and Noise . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-20
3-15 Example Traces for Troubleshooting Analysis . . . . . . . . . . . . . . . . . . . . . . . . . 3-21
4-1 Optical Unit Light Path . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4-1
4-2 UA-6 Detector Block Diagram . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4-2
4-3 Type 11 Optical Unit Functional Diagram . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4-9
4-4 Type 11 Optical Unit Schematic Diagram . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4-10
4-5 Type 11/Absorbance Detector Block Diagram . . . . . . . . . . . . . . . . . . . . . . . . . . . 4-11
5-1 Built-In Recorder Removal and Installation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5-2
5-2 Recorder String Routing . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5-3
5-3 Timing Belt Tension Adjustment . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5-5
5-4 UV Lamp and Regulating Photocell Removal and Installation . . . . . . . . . . . . . . 5-14
5-5 Window and O-Ring Removal and Installation . . . . . . . . . . . . . . . . . . . . . . . . . . 5-19
List of Tables
1-1 Technical Specifications for the UA-6 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1-3
1-2 Front Panel Controls and Indicators . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1-5
1-3 Rear Panel Controls and Connectors . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1-7
1-4 Type 11 Controls and Connectors . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1-8
2-1 Selectable Wavelengths for Type 11 Optical Unit . . . . . . . . . . . . . . . . . . . . . . . . . 2-4
3-1 O-Ring Solvent Compatibility Chart . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-2
3-2 Flow Cell Specifications . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-4
3-3 Interface Cables . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3-16
5-1 Baseline Troubleshooting . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5-6
5-2 Recorder Troubleshooting . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5-8
5-3 General Troubleshooting . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5-10
5-4 Filter Assembly Inspection . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5-16
5-5 Type 11 Optical Unit Troubleshooting . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5-16
5-6 Flow Cell Troubleshooting . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5-20

Isco UA-6
Table of Contents
xiv

1-1
UA-6 Detector
Section 1 Introduction
The UA-6 detector is a photometric detector used to measure
absorbance. It is a dual beam, dual flow cell, fixed wavelength
detector with interchangeable wavelength filters for absorbance
detection from 214 to 660 nm. Maximum sensitivity is 0.002
absorption units full-scale (AUFS) with ten ranges up to 2.0
absorption units.
The UA-6 detector consists of two components, the detector with
built-in chart recorder, variable noise filter and peak separator,
and, separated by a six-foot cable, one of two optical units. The
optical units house the lamp, filters and flow cells and are
designed to be plugged into the UA-6 and attached to a ring
stand, mast or shelf.
1.1 Optical Units for the
UA-6
1.1.1 Type 11 The Type 11 optical unit is the standard absorbance optical unit
for detection at 254 and 280 nm with eleven optional wave-
lengths to 660 nm. A mercury vapor lamp is used for all wave-
lengths.

UA-6 Detector
Section 1 Introduction
1-2
Figure 1-1 UA-6 Detector with Type 11 Optical Unit
1.2 Applications for the
UA-6
1.2.1 Liquid
Chromatography
The UA-6 is designed for chromatographic detection for low
pressure (classical) LC and high flow LC (flow rates up to
3liters/minute).
1.2.2 Cuvette Reading The UA-6 can operate as a UV/VIS computing colorimeter with
optional cuvette holders and 10 mm cuvettes.
1.3 Technical
Specifications
The technical specifications of the UA-6 are listed in Table 1-1.
1.4 Controls and
Indicators
The controls and indicators utilized in the operation of the UA-6
are illustrated in Figures 1-2 and 1-3 and described in Tables 1-2
and 1-3.

UA-6 Detector
Section 1 Introduction
1-3
Table 1-1 Technical Specifications for the UA-6
Dimensions and Weight
(Detector cabinet only)
Width: 11.2 in (28.4 cm)
Height: 6.5 in (16.5 cm)
Depth: 13.0 in (33.0 cm)
Weight:14.6 lb (6.6 kg)
Power Requirements
Mains voltage line cord is a
“disconnect device”
(Factory Set)
100 VAC ± 10 VAC, 0.4 Amps
117 VAC ± 10 VAC, 0.4 Amps
234 VAC ± 23 VAC, 0.2 Amps
Line Frequencies 50 Hz or 60 Hz (field convertible)
Power Consumption (maximum) 50 watts (117 VAC, 60 Hz), Type 11
Ambient Temperature Range 0 to 40° C
Humidity 5 to 25° C, 0 to 100% relative humidity maximum with heaters ON
(Type 11 and 12 optical unit)
10 to 40° C, 0 to 95% relative humidity maximum with heaters OFF
(Type 11 and 12 optical unit)
Full-Scale Absorbance 0.002, 0.005, 0.01, 0.02, 0.05, 0.1, 0.2, 0.5, 1.0, 2.0 Absorbance
Units Full-Scale (AUFS)
Linearity ± 1.5% of full-scale
(maximum deviation measured at mid-point of 2.0 absorbance range)
Noise Specifications for the Type 11
Optical Unit
±10 x 10-5 AU - 280 nm
±10 x 10-5 AU - 254 nm (Reference 8 x 10-5 ASTM)
(All noise specifications are for 95% total trace with dry PREP flow cells,
and 1.5 second RISE TIME setting, reference 1.5 cm/hr chart speed.)
Accuracy (Relative) 3% of full-scale, all ranges
Range-to-Range Tracking 2% of reading
Noise Filter Bessel (time delay) type. Selectable 0.5, 1.5, and 5.0 seconds RISE TIME
settings (10 to 90% full-scale response).
Stability
(baseline drift after 8 hour warm-up at
constant ambient temperature)
Type 11 optical unit:
2 x 10-3 AU maximum in 4 hours, 254 or 280 nm wavelengths.
4 x 10-3 AU maximum in 4 hours, all wavelengths.
(measured with dry prep flow cells and the same wavelength in both
optical beams)
Recorder Outputs 0 to 1 V 1% into 100K ohms or greater.
0 to 10 mV 1% into 1K ohms or greater.
Zero common mode voltage.
Recorder Input 1 V maximum internal recorder input
Integrator Output 1.0 VDC/Absorbance unit; 3% of scale plus 2% of reading

UA-6 Detector
Section 1 Introduction
1-4
Peak Separator
Peak Duration Ranges
Example: Long Duration Peak
Short Duration Peak
Maximum Slope Sensitivity
0.3, 1, 3, 10, 30+ minutes
One hour base width with a minimum height of 10% of scale
10 second base width with a minimum height of 4% of scale
0.0055% (typical) full-scale per second (positive slope 30+ minutes peak
width setting).
Sensitivity is inversely proportional to peak width setting.
Negative slope sensitivity is 1.5 times as great as positive
slope sensitivity.
The sensitivity is reduced to 3/4on the 0.01 range, 1/2on the
0.005 range and 1/4on the 0.002 range to minimize detection of
noise peaks.
Peak Separator for Fraction
Collectors
OPEN COLLECTOR Max V 30 VDC
Max I 0.5 ADC
One second TTL low standard across pins 6 and 9.
TTL low on zero to positive slope inflection and negative to zero
slope inflection.
Event Mark Inputs Open collector or contact closure.
Recorder Event Mark Display Type: Five to 10% full-scale spike added to recorder signal
Indication: Downscale for fraction collector index; upscale for accessory,
e.g. Foxy rack change.
Duration: 1/2second
Maximum Altitudes 2,000 Meters
Pollution Degree 2
Installation Category II
Built-In Recorder Chart Width: 10 cm calibrated in fifth cm minor divisions
Chart Speed: 0.3, 0.6, 1.5, 3, 6, 15, 30, 60, 150 and 300 cm/hr
Chart Paper Length: 3,000 cm/roll
Pen Lift: manual
External Chart Drive Control: contact closure across pins 1 and 2 of
rear panel connection stops the recorder chart drive
External Chart Speed Control: 300 cm/hr and slower
Operating Wavelengths of
Optical Units
Type 11: 254, 280, 310, 340, 365, 405, 435, 470, 510, 546, 580, 620 or 660
nm wavelengths
Injection Marks Input to allow externally activated injection mark to be placed on the
recorder. Activated by open collector or contact closure.
Table 1-1 Technical Specifications for the UA-6 (Continued)