Teledyne 340CF ELSD Operating instructions

340CF ELSD
Installation and Operation Guide
Part #69-5243-095
Copyright © 2006
Revision G, July 25, 2016

ELSD
Safety
iii
ELSD
Safety
General Warnings Before installing, operating, or maintaining this equipment, it is
imperative that all hazards and preventive measures are fully
understood. While specific hazards may vary according to
location and application, take heed of the following general
warnings:
WARNING
Liquids associated with this instrument may be classified
as carcinogenic, biohazard, flammable, or radioactive.
Should these liquids be used, it is highly recommended that
this application be accomplished in an isolated
environment designed for these types of materials in
accordance with federal, state, and local regulatory laws,
and in compliance with your company’s chemical/hygiene
plan in the event of a spill.
AVERTISSEMENT
Eviter de répandre des liquides dangereux. Les liquides qui
sont analysés dans cet instrument peuvent être
cancérigènes, hasards biologiques, inflammables, ou
radioactifs. Si vous devez utiliser tels liquides, il est très
recommandé que vous le faites à l'intérieur d'un
environnement isolé conçu pour tels liquides. Cet
environnement isolé devrait être construit selon les
règlements fédéraux, provinciaux, et locaux, aussi que le
plan de votre compagnie qui concerne l'évènement d'un
accident avec les matières hasardeuses.
WARNING
Avoid hazardous practices! If you use this instrument in
any way not specified in this manual, the protection
provided by the instrument may be impaired.
AVERTISSEMENT
Éviter les usages périlleux! Si vous utilisez cet instrument
d’une manière autre que celles qui sont specifiées dans ce
manuel, la protection fournie de l’instrument peut être
affaiblie; cela augmentera votre risque de blessure.
WARNING
If this system uses flammable organic solvents, Teledyne
Isco recommends that you place this system in a
well-ventilated environment, designed for these types of
materials. This environment should be constructed in
accordance with federal, state, and local regulations. It
should also comply with your organization’s plan

ELSD
Safety
iv
concerning chemical and hygiene mishaps. In all cases use
good laboratory practices and standard safety procedures.
AVERTISSEMENT
Ce système peut utiliser des dissolvants organiques
inflammables. Pour réduire le péril qui peut être causé par
l'accumulation des vapeurs explosives, Teledyne Isco
recommande que vous installez ce système dans un
environnement bien-aéré qui est conçu pour les matières
hasardeuses. Cet environnement devrait être construit
selon les règlements fédéraux, provinciaux, et locaux.
Aussi, il devrait se conformer au plan de votre organisation
qui concerne les mésaventures de l'hygiène ou de
chimique. En tout cas, utilisez toujours de pratiques
bonnes de la laboratoire et des procédures standardes de
la sûreté.
Hazard Severity Levels This manual applies Hazard Severity Levels to the safety alerts,
These three levels are described in the sample alerts below.
CAUTION
Cautions identify a potential hazard, which if not avoided, may
result in minor or moderate injury. This category can also warn
you of unsafe practices, or conditions that may cause property
damage.
WARNING
Warnings identify a potentially hazardous condition, which
if not avoided, could result in death or serious injury.
DANGER
DANGER – limited to the most extreme situations
to identify an imminent hazard, which if not
avoided, will result in death or serious injury.

ELSD
Safety
v
Hazard Symbols The equipment and this manual use symbols used to warn of
hazards. The symbols are explained below.
Hazard Symbols
Warnings and Cautions
The exclamation point within the triangle is a warning sign alerting you of
important instructions in the instrument’s technical reference manual.
The lightning flash and arrowhead within the triangle is a warning sign alert-
ing you of “dangerous voltage” inside the product.
Symboles de sécurité
Ce symbole signale l’existence d’instructions importantes relatives au pro-
duit dans ce manuel.
Ce symbole signale la présence d’un danger d’électocution.
Warnungen und Vorsichtshinweise
Das Ausrufezeichen in Dreieck ist ein Warnzeichen, das Sie darauf
aufmerksam macht, daß wichtige Anleitungen zu diesem Handbuch
gehören.
Der gepfeilte Blitz im Dreieck ist ein Warnzeichen, das Sei vor “gefährlichen
Spannungen” im Inneren des Produkts warnt.
Advertencias y Precauciones
Esta señal le advierte sobre la importancia de las instrucciones del manual
que acompañan a este producto.
Esta señal alerta sobre la presencia de alto voltaje en el interior del
producto.

ELSD
Safety
vi

vii
340CF ELSD
Table of Contents
Section 1 Quick Start Guide
1.1 Connecting to the CombiFlash® Rf200 ..................................1-1
1.2 Connecting to the CombiFlash® Torrent.................................1-4
1.3 Nebulization Chamber Drain ..........................................1-6
1.4 Basic Operation .....................................................1-6
Section 2 Introduction
2.1 Overview ..........................................................2-1
2.2 Servicing ..........................................................2-1
2.3 Operating Principles .................................................2-1
2.3.1 Nebulization ..................................................2-2
2.3.2 Evaporation ..................................................2-3
2.3.3 Detection .....................................................2-3
2.4 Specifications.......................................................2-4
Section 3 Installation
3.1 Unpacking .........................................................3-1
3.2 Connections ........................................................3-1
3.2.1 Power .......................................................3-1
3.2.2 Exhaust .....................................................3-1
3.2.3 Location .....................................................3-1
3.2.4 Communication ...............................................3-1
3.2.5 Digital I/O Connections .........................................3-2
3.2.6 Gas Connections ...............................................3-2
3.2.7 Fluid Connections .............................................3-2
3.2.8 Nebulization Chamber Drain ....................................3-2
3.3 Connection to Teledyne Isco Instruments ................................3-3
3.3.1 Connecting to the CombiFlash® Rf200 ............................3-3
3.3.2 Connecting to the CombiFlash® Rf200 with 4X Module ...............3-5
3.3.3 Connecting to the CombiFlash® Torrent Module ....................3-6
3.4 Conversion for HPLC Applications .....................................3-6
3.4.1 Operating Temperatures ........................................3-9
3.4.2 Shut Down Procedure ..........................................3-9
3.5 Installation Qualification Checklist ....................................3-10
Section 4 Operation
4.1 Instrument Controls .................................................4-1
4.1.1 Status Indicator Lights .........................................4-1
4.1.2 Status Alarm .................................................4-1
4.1.3 MENU|POWER Key ...........................................4-1
4.1.4 Autozero Key .................................................4-2
4.1.5 Display Screens ...............................................4-2
4.2 Operation ..........................................................4-9
4.2.1 Start Up Procedure ............................................4-9
4.2.2 Choosing Operating Conditions ..................................4-9

340CF ELSD
Table of Contents
viii
4.3 Operating Modes ...................................................4-12
4.3.1 Automatic Operation (Default) ..................................4-13
4.3.2 Manual Operation ............................................4-13
4.4 Mobile Phase Considerations .........................................4-13
4.5 Default Method ....................................................4-14
List of Figures
1-1 Preparing the Rf200 connection ports ...................................1-1
1-2 Connecting the Rf ports to the ELSD ...................................1-2
1-3 Connecting gas supply to ELSD .......................................1-2
1-4 Rf external detector cable connections ..................................1-3
1-5 Replace the PEEK tee with the metal tee fitting ..........................1-4
1-6 Torrent: disconnect tubing from bottom column connection .................1-5
1-7 Tee fitting for Torrent to ELSD ........................................1-5
1-8 Attach drain adaptor; prime with mobile phase if desired ..................1-6
2-1 Thermo-Split chamber: Cooling ........................................2-2
2-2 Thermo-Split chamber: Heating .......................................2-2
2-3 Evaporation and detection ............................................2-3
3-1 Attach drain adaptor; prime with mobile phase if desired ..................3-2
3-2 Preparing the Rf200 connection ports ...................................3-3
3-3 Connecting the Rf ports to the ELSD ...................................3-3
3-4 Connecting gas supply to ELSD .......................................3-4
3-5 Rf external detector cable connections ..................................3-4
3-6 Component arrangement for connection with Rf200 and 4X module ..........3-5
3-7 Coupler for connecting 4X tubing ......................................3-5
3-8 Case top removal: Remove screws ......................................3-6
3-9 ELSD Interior ......................................................3-7
3-10 Removal of Liquid Inlet plug .........................................3-7
3-11 Disconnect PTFE line from pump .....................................3-8
3-12 Disconnect pump harness ...........................................3-8
4-1 Spray Chamber Temperature Effects – Sub-ambient .....................4-10
4-2 Spray Chamber Temperature Effects – Elevated .........................4-11
4-3 Drift Tube Temperature Effects ......................................4-12
List of Tables
2-1 340CF ELSD Specifications ..........................................2-4
3-1 Suggested Operating Temperatures for HPLC Gradient Separations .........3-9
5-1 Installation Qualification Checklist ...................................3-10

1-1
340CF ELSD
Section 1 Quick Start Guide
This section provides information specific to connecting the
ELSD unit to a Teledyne Isco instrument. For general infor-
mation about installing the ELSD, refer to Section 3.2.
1.1 Connecting to the
CombiFlash® Rf200
The CombiFlash Rf200 uses RFID (Radio Frequency Identifi-
cation) technology to automate setting the parameters for purifi-
cation runs and fraction collection.
To connect the ELSD to the Rf200:
1. On the Rf, disconnect the tubing from ports C and D.
(Retain the tubing assembly in case it is needed again
later.)
Figure 1-1 Preparing the Rf200 connection ports
2. Connect the shorter tubing assembly to port C, and the lon-
ger tubing assembly to port D (refer to Figure 1-2).
3. Connect the other ends of the tubing to either side of the
tee fitting on the rear panel of the ELSD.
Disconnect
portsC&D

340CF ELSD
Section 1 Quick Start Guide
1-2
Figure 1-2 Connecting the Rf ports to the ELSD
4. Connect a 65 ±5psi inert gas supply (such as nitrogen) to
the brass fitting on the lower left rear panel of the ELSD,
with 1/8" O.D. tubing.
Figure 1-3 Connecting gas supply to ELSD
5. Connect the external detector cable (Isco part
#250-0000-20) to the Rf (Figure 1-4).
6. Connect the other end of the cable to the ELSD, taking
care to observe polarity as indicated by the small tab on
the ground side of the connector (see Figure 1-4).
183cm
60cm

340CF ELSD
Section 1 Quick Start Guide
1-3
Figure 1-4 Rf external detector cable connections
Ground
(CU)
Ground
Rf200 Connection ELSD Connection

340CF ELSD
Section 1 Quick Start Guide
1-4
1.2 Connecting to the
CombiFlash® Torrent
The CombiFlash Torrent system is designed to handle a variety
of large-scale purification needs, with various methods of sample
introduction and collection.
Connection of the Torrent to the ELSD requires the connection
package, part #60-5247-021. The package contains the following:
1. Tee fitting with reducer
2. Two 0.25" mounting spacers for tee
3. Two #6x1" pan head screws/flat washers/lock washers
4. 1 pc 1/16 OD x 0.02" ID x 6" long tubing
5. 1 pc 0.25 OD x 0.188" ID x 100cm long black PTFE tubing
w/ metal nut and ferrule swaged on each end
6. 1 pc 0.25 OD x 0.188" ID x 100cm long black PTFE tubing
w/ nut and ferrule swaged one end and a union on the
other end
To connect the ELSD to the Torrent:
1. With a 3/32" Allen wrench, remove the PEEK tee fitting and
metal tubing from the rear panel of the ELSD unit (refer to
Figure 1-5).
2. Using the two long screws with lock and flat washers,
mount the metal tee fitting on the rear panel, aligning the
two standoffs over the ELSD’s threaded mounting holes,
and the mounting holes in the tee over the two standoffs.
Figure 1-5 Replace the PEEK tee with the metal tee fitting
3. Locate the 6" piece of tubing. Attach one end to the bottom
of the metal tee fitting and the other end to the ELSD liq-
uid inlet (refer to Figure 1-7). Tighten fittings to finger,
tight.
4. On the Torrent, disconnect the black PTFE tubing end
from the bottom column connection (Figure 1-6).
Standoffs (place over
threaded holes)
Threaded
mounting holes

340CF ELSD
Section 1 Quick Start Guide
1-5
Figure 1-6 Torrent: disconnect tubing from bottom column connection
5. Locate the black PTFE tubing that is swaged on both ends.
Connect one end to the Torrent bottom column connection.
Connect the other end to the metal tee fitting on the back
of the ELSD.
6. Locate the other piece of black PTFE tubing from the kit.
Connect the metal union to the swaged tubing on the front
of the Torrent. Connect the swaged end of the tubing to the
metal tee fitting on the back of the ELSD.
Figure 1-7 Tee fitting for Torrent to ELSD
Disconnect
Union

340CF ELSD
Section 1 Quick Start Guide
1-6
1.3 NebulizationChamber
Drain
Attach the stainless steel drain tube to the nebulization chamber
drain port located on the front of the instrument. Direct the
outlet of the tube to a collection vessel and empty the vessel
when full.
Ensure that the instrument’s internal “P” trap is full during
operation. Restricting the drain port with liquid is very
important to ensure detection sensitivity. Introduce 10 mL of
mobile phase into the drain using a squirt bottle or syringe.
Figure 1-8 Attach drain adaptor; prime with mobile phase if desired
1.4 Basic Operation Startup
1. Press MENU|POWER to turn on the ELSD.
2. Following power-up diagnostics, press MENU|POWER to
begin warm-up.
3. Turn on the gas supply to 65 ±5 psi.
Wait for the asterisk (*) to stop flashing before using it.
The signal displayed should be near 20mV. If not, press
AUTOZERO.
Shutdown
Press and hold MENU|POWER until the unit shuts down (about 5
seconds).

2-1
340CF ELSD
Section 2 Introduction
2.1 Overview ELSDs are near universal detectors, primarily used in High Per-
formance Liquid Chromatography (HPLC) though they have
been used successfully in other types of chromatography as well.
Their principal requirement is that the analyte be less volatile
than the mobile phase. An ELSD cannot detect highly volatile
analytes. However, most analytes of interest are less volatile
than the eluting solvents.
Evaporative light scattering detectors complement ultraviolet
(UV) detection because they can detect most analytes, even those
that do not absorb UV radiation, are stable during gradient elu-
tions, and respond to the relative mass of the analyte – an
important feature that is useful when detecting unknown mate-
rials. The ELSD is superior to the refractive index detector
(RID), it can be used with gradient chromatography, it is not sus-
ceptible to ambient temperature changes, and it does not produce
negative peaks, which can be difficult to quantify. The ELSD does
not respond to the mobile phase disruption seen as solvent front
peaks in the void volume with UV and RI detectors, so early
eluting analytes can be easily quantified. Mass spectrometry
(MS) detection is also a universal detector, but its high cost and
complexity have kept it from being widely used.In fact, the oper-
ation requirements of MS closely match that of the ELSD. This
allows the less expensive and complicated ELSD to be used as a
method development detector for methods to be used on the MS
systems.
2.2 Servicing A periodic maintenance kit is available from Teledyne Isco (part
#60-5247-024). The kit contains instructions, parts, and
equipment for servicing the ELSD. Contact the factory for more
information.
The ELSD should be cleaned and calibrated once a year by a
qualified technician. Use water with a mild detergent, or for
tougher stains, use Isopropyl Alcohol (IPA).
2.3 Operating Principles The ELSD employs a unique method of detection. The process
involves the nebulization of the column eluent, transforming it
into an aerosol cloud. As this cloud travels through a heated zone
within the instrument, the more volatile mobile phase evapo-
rates, leaving a smaller cloud of analyte particles. These particles
pass through a beam of light, scattering some of the light, which
is converted into an electronic signal.

340CF ELSD
Section 2 Introduction
2-2
2.3.1 Nebulization Nebulization transforms the liquid phase leaving the column into
an aerosol cloud of fine droplets. The size and uniformity of the
droplets are extremely important in achieving sensitivity and
reproducibility. The ELSD uses a special concentric flow nebu-
lizer and a constant flow of an inert gas to ensure a narrow
droplet size distribution. Our nebulizer is constructed entirely
from PTFE, which accumulates deposits less than either glass or
stainless steel.
To handle flow rates and mobile phases common in HPLC, all
ELSDs need a way to divert part of the aerosol cloud to waste.
TheELSDusesapatentpendingThermo-Split technology.
Our Thermo-Split chamber combines a gentle bend with tem-
perature controlled walls. When the aerosol exiting the nebulizer
encounters a cool environment, it partially condenses into larger
particles whose momentum carries them into the wall and down
the drain.
With cooling, the particles con-
dense and increase in size. They
are carried into the walls of the
bend and exit via the drain.
Figure 2-1 Thermo-Split chamber: Cooling
With heat, the particles decrease
in size and all pass the bend in
the Thermo-Split chamber.
Figure 2-2 Thermo-Split chamber: Heating
With the Temperature control option installed, the temperature
of the Spray Chamber may be elevated. As the aerosol traverses
the chamber, it partially evaporates, shifting the particle size dis-
tribution low enough for essentially all the particles to negotiate
the bend. These operating conditions may be useful for special
applications. Under these conditions a majority of the aerosol
particles pass through the chamber and are carried into the
evaporative zone.

340CF ELSD
Section 2 Introduction
2-3
2.3.2 Evaporation After passing through the
nebulization chamber, the
aerosol cloud is propelled
through the heated evap-
oration tube, assisted by
the carrier gas. In the
evaporation tube, the
solvent is volatilized to
produce particles or
droplets of pure analyte.
The temperature of the
drift tube is set at the
temperature required to
evaporate the solvent.
The temperature is kept
as low as possible to avoid
particle shape distortion,
evaporation of the
analyte or when working
with thermally sensitive
compounds.
Figure 2-3 Evaporation and detection
2.3.3 Detection The particles emerging from the evaporation tube enter the
optical cell, where the sample particles pass through the light
emitted by a low-power laser. The particles scatter the light,
which is detected by a silicon photodiode located at a 90° angle
from the laser. A light trap is located opposite the laser to collect
the light not scattered by particles. The quantity of light detected
is proportional to the solute concentration and solute particle
size distribution. The photodiode produces a signal, which is sent
to the outputs for collection.

340CF ELSD
Section 2 Introduction
2-4
2.4 Specifications
Table 2-1 340CF ELSD Specifications
Dimensions 10" W x 18.5" D x 11.5" H
Weight 25 lbs
Display 2 Line x 20 Character per line VFD
User Interface Four multi-function keys
Evaporative Zone Temperature Ambient to 120 °C
Thermo-Split™ Chamber Temperature 10 °C to 70 °C
Liquid Flow Rate ~ 0.75 mL/min
Gas Requirements 65 psi ± 5 psi Nitrogen or other inert gas
Gas Consumption ~ 2.5 SLPM
Operating Conditions Intended for indoor use only, 15.6 to 26.7 °C
(60 to 85 °F) and <90% R.H. non condensing
Electrical Requirements Nominal 120 VAC, 50/60 Hz or
Nominal 234 VAC, 50/60 Hz;
600 watts
Fuse Size
120 VAC
240 VAC
6.25A
3.15A
Wetted Materials Stainless steel, glass, anodized aluminum,
Teflon®
Light Source 670 nm Laser Diode, <5 mW
Detector Hermetically sealed photo-diode/operational
amplifier
Output Signal 0–5 VDC
Interface RS232, Contact Closure
Installation Category II
Pollution Degree 2
Altitude (max.) 2,000 M

3-1
340CF ELSD
Section 3 Installation
3.1 Unpacking Unpack carefully and confirm that all the items are present. The
ELSDs come in a high quality shipping container, engineered to
avoid damage in transit. Save the shipping container and pack-
aging for future shipments.
3.2 Connections The following sections provide general connection information for
the ELSD unit. For information about connecting the ELSD to
Teledyne Isco instruments, refer to Section 3.3.
3.2.1 Power The ELSD operates at 120V or 234V, 50/60 Hz. Confirm that the
ELSD is configured for the correct voltage before plugging it into
line voltage. The voltage configuration is listed on the serial tag.
If the voltage listed is incorrect, please contact your local
Teledyne Isco representative.
Plug the modular power cord provided into the power input
module on the back of the detector. The power cord is the dis-
connect device for the unit. Therefore, select a power outlet that
is nearby and unobstructed, allowing easy access for discon-
nection in the event of an emergency.
3.2.2 Exhaust The ELSD exhaust is a ½" O.D. stainless steel tube. Use ½" I.D.
tubing to connect to a fume hood during operation. Alternately,
connect tubing and direct to a cooled collection vessel for later
disposal.
3.2.3 Location The ELSD rear panel has a fan for cooling. Position the
instrument so that the rear panel is at least 5cm from any wall or
object, to allow free movement of air.
3.2.4 Communication
An output jack can be found on the back of the ELSD for con-
nection to The CombiFlash Rf200. The maximum signal output is
5V.
CAUTION
Observe polarity when connecting to this output. Refer to
Section 3.3 for complete details about connection.
Analog Voltage Output
Circuit
common

340CF ELSD
Section 3 Installation
3-2
3.2.5 Digital I/O
Connections
Pin 1 and Pin 2: Input for remote control of pump (refer to
Section 4.3). Also a contact closure output. An error conditions
exists when pins are open.
Pin3andpin4:A contact closure input to turn on/off gas
remotely. Gas control toggles with electrical continuity between
these two pins. Maintain connectivity for a minimum of 500mS.
A subsequent toggle command within 2 seconds may be ignored.
Pin 5 and 6: A TTL output for instrument status. A logical high
indicates the detector is in standby.
Pin 7 and pin 8: A contact closure input to reset baseline
remotely. Maintain connectivity for a minimum of 500mS. A sub-
sequent toggle command within 2 seconds may be ignored.
3.2.6 Gas Connections Connect a supply of clean, dry, inert gas regulated to 65psi ± 5 psi
to the GAS INLET port on the back of the unit.
WARNING
Use only Nitrogen or Argon.
The internal gas regulator maintains the gas pressure at the
factory set value displayed on the front panel.
3.2.7 Fluid Connections Connect the outlet from your column to the LIQUID INLET on the
front of the unit. The length and volume between the column
outlet and the detector inlet should be kept as short as possible
to avoid unnecessary band broadening.
3.2.8 Nebulization Chamber
Drain
Attach the stainless steel drain tube to the nebulization chamber
drain port located on the front of the instrument. Direct the
outlet of the tube to a collection vessel and empty the vessel
when full.
Ensure that the instrument’s internal “P” trap is full during
operation. Restricting the drain port with liquid is very
important to ensure detection sensitivity. Introduce 10 mL of
mobile phase into the drain using a squirt bottle or syringe.
Figure 3-1 Attach drain adaptor; prime with mobile phase if desired
Rear Panel Connector
for Digital I/O
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