Thermo Electron IEC CL40 Series User manual

IEC CL40 - FL40 Series
User’s Manual
Analyze •Detect •Measure •ControlTM

IEC CL40 - FL40 Series
User’s Manual
CL40 - FL40 Series

Carefully read this manual before operating your instrument.
Information contained in this document is the property of Thermo Electron Corporation; it may not be
duplicated or distribuited without the owner’s authorization.
The validity of the guarantee is subject to the observation of the instructions
and precautions described in this document.

Thermo Electron Corporation
MANUAL NUMBER
REV ECR/ECN DATE DESCRIPTION BY
a 11290088 03/06 Initial release
Preface
CL40 - FL40 i

ii CL40 - FL40 Thermo Electron Corporation
Packing List
Item number Quantity Check
Centrifuge 
11290088 User manual 1 
CE Declaration of compliance 1 
Manual lid opening instructions 1 
Imbalance protection instructions 1 
64227001 Mains cord 1 
26227005 Toolbox:
26339005 • Rotor removal tool 1 
85241084 • Emergency unlooking tool 1 
86000897 • 12 and 13 mm wrench 1 
25055501 • 21 mm wrench 1 
For missing parts call your nearest Thermo representative
11210922 CL40, 230V 50/60 Hz
11210923 CL40 120V 60 Hz
11210924 CL40 100V 50/60 Hz
11210926 CL40R 230V 50/60 Hz
11210927 CL40R 120V 60 Hz
11210928 CL40R 100V 50 Hz
11210929 CL40R 100V 60 Hz
11210940 FL40 230V 50/60 Hz
11210941 FL40 120V 60 Hz
11210942 FL40 100V 50/60 Hz
11210943 FL40R 230V 50/60 Hz
11210944 FL40R 120V 60 Hz
11210945 FL40R 100V 50 Hz
11210946 FL40R 100V 60 Hz

CL40 - FL40 iiiThermo Electron Corporation
WEEE Compliance
WEEE Compliance. This products is required to comply with the
European Union’s Waste Electrical & Electronic Equipment (WEEE)
Directive 2002/96EC. It is marked with the following symbol. Thermo
Electron has contracted with one or more recycling/disposal companies
in each EU Member State, and this products should be disposed of or
recycling through them. Further information on Thermo Electron’s
compliance with these Directives, the recyclers in your country, and
information on Thermo Electron products which may assist the detec-
tion of substances subject to the RoHS Directive are available at
www.thermo.com/WEEERoHS
WEEE Konformittät. Dieses Produkt muss die EU Waste Electrical &
Electronic Equipment (WEEE) Richtlinie 2002/96ECerfüllen. Das
Produkt ist durch folgendes Symbol gekennzeichnet. Thermo Electron
hat Vereinbarungen getroffen mit Verwertungs-/Entsorgungsanlagen in
allen EU-Mitgliederstaaten und dieses Produkt muss durch diese
Firmen widerverwetet oder entsorgt werden. Mehr Informationen über
die Einhaltung dieser Anweisungen durch Thermo Electron, die
Verwerter und Hinweise die Ihnen nützlich sein können, die Thermo
Electron Produkte zu identizfizieren, die unter diese RoHS Anweisung
fallen, finden Sie unter www.thermo.com/WEEERoHS
Conformità WEEE. Questo prodotto deve rispondere alla direttiva
dell’Unione Europea 2002/96EC in merito ai Rifiuti degli Apparecchi
Elettrici ed Elettronici (WEEE). È marcato col seguente simbolo.
Thermo Electron ha stipulato contratti con una o diverse società di
riciclaggio/smaltimento in ognuno degli Stati Membri Europei. Questo
prodotto verrà smaltito o riciclato tramite queste medesime. Ulteriori
informazioni sulla conformità di Thermo Electron con queste
Direttive, l’elenco delle ditte di riciclaggio nel Vostro paese e informa-
zioni sui prodotti Thermo che possono essere utili alla rilevazione di
sostanze soggette alla Direttiva RoHS sono disponibili sul sito
www.thermo.com/WEEERoHS
WEEE. Este producto requiere cumplir con la Directiva 2002/96EC
WEEE (residuos de quipos electricos y electronicos). Este producto esta
marcado con el simbolo WEEE, como se describe. En cada Estado
Miembro de la Union Europea (EU), Thermo Electron ha contratado
con diversas companias para disponer y/o re-ciclar los residuos de equi-
pos electricos y electronicos. Existe information adictional acerca de la
conformidad de Thermo Electron con esta Directiva, incluyendo los
nombres de las diversas companias autorizadas para disponer/recyclar
nuestros productos. Adicionalmente suministramos informacion acerca
Great Britain
Deutschland
Italia
Espana

iv CL40 - FL40 Thermo Electron Corporation
WEEE Compliance
de los productos de Thermo Electron que puedes asistir en la deteccion
de substancian mensionades en la directiva RoHS, explicado en
www.thermo.com/WEEERoHS
Conformité WEEE. Ce produit doit être conforme à la directive euro-
péenne (2002/96EC) des Déchets d’Equipements Electriques et
Electroniques (DEEE). Il est merqué par le symbole suivant. Thermo
Electron s’est associé avec une ou plusieurs compagnies de recyclage
dans chaque état membre de l’union européenne et ce produit devrait
être collecté ou recyclé par celles-ci. Davantage d’informations sur la
conformité de Thermo Electron à ces directives, les recycleurs dans
votre pays et les informations sur les produits Thermo Electron qui
peuvent aider le détection des substances sujettes à la directive RoHS
sont disponibles sur www.thermo.com/WEEERoHS
France

CL40 - FL40 vThermo Electron Corporation
Declaration of compliance
We, Thermo Electron Industries S.A.S., Z.I. d’Aze Bellitourne 53200
CHATEAU-GONTIER, France, declare that the instrument defined
here below complies with the following European directives:
Nous, Thermo Electron Industries S.A.S., Z.I. d’Aze Bellitourne 53200
CHATEAU-GONTIER, France, déclarons que le matériel désigné ci-
après est conforme aux directives européennes suivantes:
Wir, Thermo Electron Industries S.A.S. , Z.I. d'Aze Bellitourne 53200,
CHATEAU-GONTIER, France, erklären dass das hier beschriebene
Gerät sich in Übereinstimmung mit folgenden europäischen
Richtlinien befindet:
Nostros, Thermo Electron Industries S.A.S., Z.I. d’Aze Bellitourne
53200 CHATEAU-GONTIER, France, declaramos que el instrumento
bajo descrito está fabricado según las especificaciones establecidas por
las siguientes normas europeas:
Nós, Thermo Electron Industries S.A.S., Z.I. d’Aze Bellitourne 53200
CHATEAU-GONTIER, France, declaramos que o instrumento aqui
apresentado está de acordo com as normas européias abaixo listadas:
Noi, Thermo Electron Industries S.A.S., Z.I. d’Aze Bellitourne 53200
CHATEAU-GONTIER, France, dichiariamo che lo strumento sotto
descritto è conforme alle seguenti Direttive europee:
• Machinery: 98 / 37 / CEE
• Low voltage: 72 / 23 / CEE - 93 / 68 CEE
• EMC: 89 / 336 CEE - 92 / 31 CEE - 93 / 68 CEE
• Safety standard: EN 61010-1 - EN 61010-2-020
Château-Gontier, 28th June 2005
Managing Director
Christian Galiana
Declaration de conformite
Declaration of compliance
Konformitätserklärung
Declaración de conformidad
Declaraçao de conformidade
Dichiarazione di conformità

vi CL40 - FL40 Thermo Electron Corporation
Guarantee Terms
Thermo guarantees that this unit is free from defects in materials and
workmanship when it leaves the factory, and will replace or repair the
unit if it proves defective in normal use or during service for a period of
ONE YEAR from the delivery.
This guarantee is invalid if the unit is incorrectly used, poorly serviced
or neglected, mis-used or accidentally damaged.
There is no explicit guarantee other than as stated above.

CL40 - FL40 viiThermo Electron Corporation
For further information,
assistance or service
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Other Europe, Middle East, or Africa
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In Other Countries:
Contact your local THERMO products distributor
or agent.
E-mail Technical Service Representative for
products at:
[email protected]
Visit our web site at: www.thermo.com

viii CL40 - FL40 Thermo Electron Corporation
Contents
Use and Function........................................................................................1-1
User Manual......................................................................................1-1
Warning Symbols ..............................................................................1-1
Description ......................................................................................1-1
Refrigeration and Heating Systems....................................................1-2
Drive System ....................................................................................1-3
Safety Interlock System ....................................................................1-3
Imbalance Detection System ............................................................1-3
Relative Centrifugal Force ................................................................1-4
Specifications ............................................................................................2-1
Dimensions and Weight....................................................................2-1
Centrifugation Specifications ............................................................2-1
Electrical Specifications ....................................................................2-2
Installation ..................................................................................................3-1
Environmental Conditions................................................................3-1
Unpacking ........................................................................................3-1
Positioning ........................................................................................3-2
Mains Supply ....................................................................................3-2
Lid Opening and Rotor Checking ....................................................3-3
Manual Lid unlocking Procedure ......................................................3-3
Performance and Accessories ............................................................3-5
M4 Swing-out Rotor - Cat. N°: 11175338 ......................................3-5
Biocontainement Accessories ............................................................3-6
QG2.5 Swing-out Rotor - Cat. N°: 11175382..................................3-8
Angle Rotors ....................................................................................3-8
Glassware ........................................................................................3-10
Instructions for Use....................................................................................4-1
Controls and Indicators ....................................................................4-1
Display Screens ................................................................................4-2
Control Panel Functions ..................................................................4-3
Control Panel Indicators ..................................................................4-4
Description of Certain Events with Respect to the Control System ..4-5
Preparing the First Run of the Day ..................................................4-7
Sample Loading ................................................................................4-7
Chapter 1
Chapter 2
Chapter 3
Chapter 4

CL40 - FL40 ixThermo Electron Corporation
Contents
Hazards, Precautions and Limitations of Use ......................................5-1
IEC 1010-2-020................................................................................5-1
Cautions............................................................................................5-1
Speed Control ..................................................................................5-2
Operational Limitations ....................................................................5-2
Aerosol Risks ....................................................................................5-2
Service and Maintenance ........................................................................6-1
Periodic Cleaning ..............................................................................6-1
Contamination Hazards ....................................................................6-1
Disinfection ......................................................................................6-2
Radioactive Decontamination ..........................................................6-2
Power Supply Circuit Breaker............................................................6-3
Rotor Removal ..................................................................................6-3
Trunnion Lubrication........................................................................6-3
Chemical Compatibility Table for Rotors, Tubes and Accessories......6-4
Disposal of Product....................................................................................7-1
Chapter 5
Chapter 6
Chapter 7

Chapter 1 Use and Function
The user manual is part of the centrifuge, and contains important
information for your safety and for the best use of the equipment
Always keep the manual close to the appliance and in a safe place, so
that it is always available. Thermo strongly recommends that all users
read this manual carefully.
The following symbols are provided to help the operator take
advantage of the protection afforded by the equipment and to warn of
potential danger.
On the main switch, allows centrifuge to operate. ▲
On the main switch, disconnects the centrifuge from the main power
supply. ▲
Only authorized personnel can touch the parts close to this symbol
and, in any case, only after switching off the main power supply.
Coming in contact with high voltages could cause severe injuries. ▲
In this manual, this symbol means that you will find important
information for safety, which if unobserved could result in damage to
the appliance and/or harm to the operator. ▲
In this manual, this symbol means that you will find important
information about minimizing biological risk: if unobserved the result
could be harm to the operator. ▲
The CL40 and FL40 centrifuges are designed for laboratory use.
They will separate the components of fluids into layers of varying
density by subjecting them to high forces.
Centrifugal force provided by the centrifuge can also be used to drive
solvents and low molecular weight solutes through the membrane of a
filtering device. Retained macrosolutes will therefore be found above
the membrane.
Relative Centrifugal Force (RCF) generated by a rotor is directly
User Manual
Warning Symbols
Description
CL40 - FL40 1-1Thermo Electron Corporation

Chapter 1
Use and Function
1-2 CL40 - FL40 Thermo Electron Corporation
proportional to its sedimentation useful radius and to the value of its
speed squared.
A control system permits the user, through an extremely intuitive
control panel, to set and control the speed, the g-force, the temperature
(on the thermostated and refrigerated versions) and the run time, as
well as to view different messages and warnings.
The CL40 and FL40 are ventilated unit. A permanent air circulation
reduces the heat level in the bowl.
However the temperature in the bowl can reach 10 °C above the
ambient temperature after hours of continuous operation.
The CL40R and FL40R are equipped with a powerful refrigeration
system that allows samples to be processed at low temperature. The
refrigerant, R134a, is free of CFC (Chloro-Fluoro-Carbons) in
accordance with the Montreal Protocol directions for preservation of the
ozone layer in the atmosphere.
The actual temperature in the bowl is continuously displayed on the
front panel of the centrifuge. Under certain conditions (high speed,
long duration runs) the sample temperature can be higher than the
bowl temperature on the display. In this case, the user may compensate
manually after empirical determination of the temperature differential.
The lid should remain closed when the refrigeration system is in use to
avoid condensation.
The centrifuge has a rubber hose that allows condensation to be
drained from the bowl. The drain hose exits at the back of the
machine. Near the drain hose you can find the following label:
To remove condensation, please follow this procedure:
• Switch off the centrifuge and disconnect the power.
• Put a small basin under the hose.
• Unplug the hose.
Refrigeration and
Heating Systems
Condensation drain on
refrigerated units

CL40 - FL40 1-3Thermo Electron Corporation
Chapter 1
Use and Function
• Drain the centrifuge bowl.
• Insert the plug back into the hose.
Attention Do not remove the plug from the drain hose during
centrifugation. ▲
A three phase brushless motor drives the rotating equipment. The rotor
is contained in a sealed, armor plated centrifugation chamber.
The brushless motor requires no maintenance. A control system
ensures the correct drive speed, which is continuously monitored.
The CL40 and FL40 centrifuge series are equipped with an interlock
system that assures two basic safety features:
• The run cannot be started if the lid is not correctly closed.
• The centrifuge lid cannot be opened if the rotor is running.
The centrifuge will not operate until the lid is closed and latched in
place.
The lid remains latched until the rotor stops spinning. The lid access
lamp indicates when the rotor has stopped: consequently the handle on
the right hand side of the centrifuge can be used to open the lid.
If a power failure occurs, access to the samples in the centrifuge is
possible via a manual lid opening procedure (see appropriate
paragraph). For this operation a special tool (supplied with the
centrifuge) is required.
Bypass the interlock system only under emergency conditions as the
rotor could still be rotating.
The CL40 and FL40 centrifuge series are equipped with a load
imbalance detector.
In case of excessive imbalance, the load imbalance indicator LED is
illuminated and the brake is applied immediately. The rotor will be
decelerated to rest in few seconds.
As soon as the motor stops, open the centrifuge and redistribute the
samples to produce an equal weight on diametrically opposite sides.
If the message persists, despite your efforts to balance the load, call
your Thermo Service representative.
Drive System
Safety Interlock
System
Imbalance Detection
System

Chapter 1
Use and Function
1-4 CL40 - FL40 Thermo Electron Corporation
Relative Centrifugal
Force
Note Imbalance tolerance depends upon the rotor in use. The
centrifuge will tolerate 15 gr. of imbalance with the M4 rotor equipped
with standard buckets. Carefully balance the sample load to avoid
actuating the imbalance detection system. If the protection has not
been removed from the imbalance detection device during installation,
the centrifuge will consider this as an imbalance situation and will not
operate.
Relative Centrifugal Force (RCF), at the circumference of a rotor and
bucket combination, is directly proportional to the speed (r.p.m.) and
radius of the rotor. Therefore, a greater r.p.m. and/or a larger radius
produces a greater RCF and improved faster separation of substances.
The centrifuge control system carries out and displays the results of all
calculations related to speed, radius and RCF.
Note : The value introduced for the radius can be adjusted to allow for
position within the tube such as at a boundary. Maximum radii are
quoted in the specifications tables. Use of improper radius will adjust
the speed setting, automatically applying the wrong RCF.

CL40 - FL40 2-1Thermo Electron Corporation
Chapter 2 Specifications
Dimensions and Weight
CL40 FL40 CL40R FL40R
Product Dimensions
Height - lid open (cm / in) 92/ 36.2 134/ 58.8 92/ 36.2 134/ 58.8
Height - lid closed (cm / in) 42/ 16.5 86/ 33.8 42/ 16.5 86/ 33.8
Width (cm / in) 58/ 22.8 58/ 22.8 85/ 33.5 58/ 22.8
Depth (cm / in) 66/ 26.0 66/ 26.0 66/ 26.0 66/ 26.0
Weight unpacked (kg / lb.) 80/176 95/209 106/233 115/253
Packing
Height (cm / in) 50/ 19.7 99/ 38.9 50/ 19.7 99/ 38.9
Width (cm / in) 70/ 27.6 68/ 26.7 97/ 38.2 68/ 26.7
Depth (cm / in) 67/ 26.4 78/ 30.7 67/ 26.4 78/ 30.7
Weight packed (kg / lb.) 91/201 120/264 116/256 145/319
Centrifugation Specifications
CL40 FL40 CL40R-FL40R
Max capacity Swing-out (4x750 mL) 3000 mL (4x750 mL) 3000 mL
Fixed Angle (8x100 mL) (8x100 mL)
Max density 1200 kg/m3 1200 kg/m3
Max load 3.6 kg 3.6 kg
Max speed Swing-out (M4) 4000 rpm 4500 rpm
Swing-out (QG2.5) 5400 rpm 5400 rpm
Fixed Angle 7800 rpm 8000 rpm
Max RCF Swing-out (M4) 3810 x g 4822 x g
Swing-out (QG2.5) 4140 x g 4140 x g
Fixed Angle 9862 x g 10375 x g
Max noise < 66 dBA < 62 dBA
CL40 FL40 CL40R-FL40R
Microprocessor control yes yes
Programs (direct recall) 5 5
Speed set/display Range 500 - 7800 500 - 8000
Step 10 rpm at less than 1000 rpm 10 rpm at less than 1000 rpm
100 rpm at above 1000 rpm 100 rpm at above 1000 rpm
Accuracy ± 20 rpm (display) ± 20 rpm (display)

2-2 CL40 - FL40 Thermo Electron Corporation
Chapter 2
Specifications
CL40 FL40 CL40R-FL40R
RCF set/display Range 20 - 9900 20 - 10400
Step 10 x g at less than 1000 x g 10 x g at less than 1000 x g
100 x g at above 1000 x g 100 x g at above 1000 x g
Accuracy ± 20 x g (display) ± 20 x g (display)
Timer set/display 30 s - 99 min + continuous 30 s - 99 min + continuous
Temperature set/display - -9°C to +40°C -9°C to +60°C
Acceleration rates 5 5
Braking rates 5 5
Drive system direct direct
Brushless induction motor yes yes
Electrical specifications
CL40 FL40 CL40R-FL40R
Nominal operating voltage 230V 230 V ±10% 230 V ±10%
120V 120 V ±10% 120 V ±10%
Power requirements (maximum) 1200 W 1500 W
Heat output (maximum) 3750 BTU / h 4435 BTU / h