Thermo Scientific 75007202 User manual

Instruction Manual
50143327-b • 05 / 2014
Thermo Scientific
Sorvall ST 8 / 8R Centrifuge

Instruction Manual
50143327-b • 05 / 2014
Thermo Scientific
Sorvall ST 8 / 8R Centrifuge

ThermrlFishsr
SCIENTIFIC
Ko N Fo RM rrÄrs E RKLAnU NG
DECLARATION OF CONFORMITY
Name und Anschrift des Herstellers und des Bevollmächtigten
für die Zusammenstellung der relevanten technischen Unterlagen:
Name and address of the manufacturer and of the authorized
representative to compile the relevant technical documentation:
Thermo Electron LED GmbH
Zweig niederlassu n g Osterode
Am Kalkberg
37520 Osterode am Harz
Germany
Hiermit erklären wir, dass die nachstehend beschriebene Maschine
Herewith we declare, that the machinery described below
mit allen einschlägigen Bestimmungen der Richtlinie über ln-vitro-Diagnostika 98n9/EG in Ühereinstimmung ist.
is in conformity with all relevant terms of directive for in vitro diagnostic medicaldevices 98n9/EC.
Die Maschine ist auch in Übereinstimmung mit allen einschlägigen Bestimmungen der Richtlinie 2A04/108/EG über
e le ktrom ag nefische Ve rlräg Iich ke it.
The machinery is in accordance with all relevant terms of directives for electromagnetic compatibility 2004/108/EC.
Die Schutzziele nach Anhang 1 der Maschinenrichtlinie 2006/42/EG und der Niederspannungsrichtlinie 2006/95/EG werden
eingehalten.
The protection goals of Annex 1 for the directives machinery 2006/4AEC and low voltage 2006/95/EC are met.
Der oben beschriebene Gegenstand der Erklärung ertüilt auch die Vorschriften der Richtlinie 2011/65/EU
des Europäischen Parlaments und des Rafes vom 8. Juni 2011 zur Beschränkung der Verwendung
bestimmter gefährlicher Sfofia in Elektro- und Elektronikgeräten. Die alleinige Verantwortung für die Ausstellung dieser
Konformiätserklärung trägt der Hersteller
The object of the declaration described aboveis a/so in conformity with Directive 2011/65/EU
of the European Parliament and of the Council of I June 2011 on the restiction of the use
of certain hazardous suösfances in electrical and electronic equipment^
This declaration of conformity is issued under the sole responsibility of the manufacturer
Angewandte harmonisierte Normen/
Harmonized standards used:
EN 61010-1:2004
EN 61010-2-020:2006
EN 61010-2-101:2002
EN 61326-1:2006
Osterode am Haz, den 10.04.2014
((
Beschreibuns / description : Labor- Zentrifuge mit Zubehör / centrifuse with accessories
Marke / brand : Sorvall
Modellbezeichnunq / model name : STSR
Modell Nr. / model no. :75007204
Gültig ab Equipmentnr.
Valid from equipment no.
: 41636211
Name Datum Dokument
Erstellt Lienemann 10.04.2014 50144526 00
Freioeoeben Laaboubi 10.o4.2014

WEEE Compliance
This product is required to comply with the European Union’s Waste Electrical & Electronic Equipment
(WEEE) Directive 2002/96/EC. It is marked with the following symbol:

Thermo Scientific
Sorvall ST 8 / 8R Centrifuge |5
WEEE Compliance......................................................... 4
Preface ................................................................. 7
Items Supplied .................................................................7
Intended Use ...................................................................7
Precautions ....................................................................8
Preparation.............................................................................8
Hazardous Substances ....................................................................9
Operating ..............................................................................9
Maintenance............................................................................9
Symbols used on the centrifuge ..................................................10
Symbols used in the manual .....................................................10
Technical Specifications .................................................. 11
Technical Data ................................................................11
Sorvall ST 8 ...........................................................................11
Sorvall ST 8R ..........................................................................12
Directives, Standards and Guidelines ..............................................13
Mains Supply .................................................................14
Rotor Selection ................................................................14
Transport and Set Up ..................................................... 15
Before Setting Up ..............................................................15
Location......................................................................15
Transporting ..................................................................15
Leveling ......................................................................16
Mains Connection ..............................................................16
Storage ......................................................................17
Shipping .....................................................................17
Control Panel ........................................................... 19
Sorvall ST 8 ...................................................................19
Sorvall ST 8R..................................................................20
Operation .............................................................. 21
Switching on the Centrifuge .....................................................21
Open the Centrifuge Door........................................................21
Close the Centrifuge Door .......................................................21
Rotor Installation ..............................................................22
Acceleration / Deceleration Rates .................................................23
Pre-Selecting Speed/RCF .................................................................24
Running Time Pre-Selection................................................................24
Continuous Operation ....................................................................25
Preselecting the Temperature ..............................................................25
Prewarming or Precooling the Centrifuge ......................................................25
Programs.....................................................................26
Table of Contents

Table of Contents
6|Sorvall ST 8 / 8R Centrifuge
Thermo Scientific
Saving a Program .......................................................................26
Selecting a Program .....................................................................26
Centrifugation .................................................................26
Maximum Loading ......................................................................26
Starting the Centrifuge Run ................................................................27
Imbalance Indicator......................................................................27
Stopping the Centrifuge Run ...............................................................28
Short-term Centrifugation .......................................................28
Removing the Rotor ............................................................28
Aerosol-tight Rotors ............................................................29
Switch off Centrifuge ...........................................................29
System Menu ........................................................... 31
Flowchart System Menu.........................................................31
Maintenance and Care .................................................... 33
Cleaning Intervals..............................................................33
Basics .......................................................................33
Rotor and Accessories Inspection............................................................33
Cleaning .....................................................................34
Cleaning the Filter Mat ...................................................................35
Disinfection ...................................................................35
Decontamination...............................................................36
Autoclaving ...................................................................36
Service of Thermo Fisher Scientific. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .37
Shipping and Disposal ..........................................................37
Troubleshooting ......................................................... 39
Mechanical Emergency Door Release ..............................................39
Troubleshooting by Guide........................................................40
When to contact Customer Service ...............................................42
Chemical Compatibility Chart .............................................. 43
Index .................................................................. 49

Thermo Scientific
Sorvall ST 8 / 8R Centrifuge |7
Before starting to use the centrifuge, read through this instruction manual carefully and follow the instructions.
Failure to follow the instructions and safety information in this instruction manual will result in the expiration of the sellers
warranty.
Items Supplied
Item Quantity
Thermo Scientific™Sorvall™ST 8 / 8R Centrifuge 1
Power Supply Cable 1
Instruction Manual 1
CD 1
If any parts are missing, please contact the nearest Thermo Fisher Scientific representative.
Intended Use
This centrifuge is a laboratory product used to separate substance mixtures of different densities.
This centrifuge can become an In-vitro-diagnostics device (Directive 98/79/EC), if used together with the hematocrit rotor
and his accessories.
The hematocrit value is determined using the instructions written in the instruction manual of the hematocrit rotor. The
instructions are based upon the reference method following DIN 58933, allowing to determinate the erythrocyte volume
fraction within the blood.
This centrifuge has to be operated by trained specialists only.
Preface
P

Precautions
8|Sorvall ST 8 / 8R Centrifuge
Thermo Scientific
Precautions
฀ WARNING
Observe the safety instructions. Not following these instructions can cause damage.
The centrifuge is to be used for its intended use only. Improper use can cause damages, contamination, and injuries with
fatal consequences.
The centrifuge should be operated by trained specialists only.
In order to ensure safe operation of the Thermo Scientific Sorvall ST 8 / 8R Centrifuge, general safety regulations must be
followed. Mind the regulations in your country.
Set Up Conditions
฀ WARNING
Plug the centrifuge only into sockets which have been properly grounded.
Turn off the centrifuge at the main switch. The mains plug must be freely accessible at all times.
Press the STOP key to shut down the centrifuge.
Pull out the power supply plug or disconnect the power supply in an emergency.
As safety zone maintain a clear radius of at least 30 cm around the centrifuge.
Do not place any dangerous substances within this security zone.
Set up in a well-ventilated environment, on a horizontally levelled and rigid surface with adequate load-bearing
capacity.
Preparation
฀ WARNING
It is the obligation of the operator to make sure, that the proper protective clothing is used. Mind the “Laboratory
Biosafety Manual” of the World Health Organization (WHO) and the regulations in your country.
Do not make any changes to the mechanical components of the rotor.
Do not touch the electronic components of the centrifuge nor alter any electronic or mechanical components.
Use only with rotors which have been properly installed. Follow the instructions on the Thermo Scientific™Auto-
Lock™rotor exchange in section “Rotor Installation” on page 22.
Do not use rotors, buckets or accessories which show any signs of removed protective coating, corrosion or
cracks. Contact customer service for further advice or inspections.
Use only with rotors which have been loaded properly.
Never overload the rotor.
Always balance the samples.
Use only rotors and accessories for this centrifuge which have been approved by Thermo Fisher Scientific.
Exceptions to this rule are commercially available glass or plastic centrifuge labware, provided they have been
designed to fit rotor or adapter cavities and are approved for the speed or the RCF value of the rotor.
Make sure the rotor is locked properly into place before operating the centrifuge.
Implement measures which ensure that no one can approach the centrifuge for longer than absolutely necessary
while it is running.
If used for foodstuffs machinery, for cosmetics or pharmaceutical products, only use closed or aerosol-tight
containers for centrifugation.

Precautions
Thermo Scientific
Sorvall ST 8 / 8R Centrifuge |9
Hazardous Substances
฀ WARNING
Especially when working with corrosive samples (salt solutions, acids, bases), the accessory parts and vessel have
to be cleaned thoroughly.
Do not centrifuge explosive or flammable materials or substances.
The centrifuge is neither inert nor protected against explosion. Never use the centrifuge in an explosion-prone
environment.
Do not centrifuge toxic or radioactive materials or any pathogenic micro-organisms without suitable safety
precautions.
If centrifuging any hazardous materials mind the “Laboratory Biosafety Manual” of the World Health Organization (WHO)
and any local regulations. When centrifuging microbiological samples from the Risk Group II (according to the “Laboratory
Biosafety Manual” of the World Health Organization (WHO)), aerosol-tight biological seals have to be used. Look on the
internet page of the World Health Organization (www.who.int) for the “Laboratory Biosafety Manual”.
For materials in a higher risk group, extra safety measures have to be taken.
If toxins or pathogenic substances have contaminated the centrifuge or its parts, appropriate disinfection measures
have to be taken (“Disinfection” on page 35).
Extreme care should be taken with highly corrosive substances which can cause damage and impair the
mechanical stability of the rotor. These should only be centrifuged in fully sealed tubes.
If a hazardous situation occurs, turn off the power supply to the centrifuge and leave the area immediately.
Operating
฀ WARNING
Never use the centrifuge if parts of its cover panels are damaged or missing.
Never start the centrifuge when the centrifuge door is open.
Do not move the centrifuge while it is running.
Do not lean on the centrifuge.
Do not place anything on top of the centrifuge during a run.
Never open the centrifuge door until the rotor has come to a complete stop and this has been confirmed in the
display.
The emergency door release may be used in emergencies only to recover the samples from the centrifuge, e.g.
during a power failure (see section “Mechanical Emergency Door Release” on page 39).
Do not open the centrifuge, while it is running.
In any case of severe mechanical failure, such as rotor or bucket crash, the centrifuge is not aerosol-tight.
In case of rotor failure the centrifuge can be damaged. Leave the room. Inform customer service.
Maintenance
฀ WARNING
The centrifuge housing is not to be opened by the operator.

Symbols used on the centrifuge
10 |Sorvall ST 8 / 8R Centrifuge
Thermo Scientific
Symbols used on the centrifuge
This symbol refers to general hazards.
CAUTION means that material damage could occur.
WARNING means that injuries or material damage or contamination could occur.
This symbol refers to biological hazards.
Observe the information contained in the instruction manual to keep yourself and your
environment safe.
This symbol refers to information on hazards, described within the manual.
This symbol demands to disconnect mains before transporting or servicing the centrifuge.
This Symbol demands to check, if the rotor is installed correct by lifting it slightly at the handle.
See “Rotor Installation” on page 22.
Symbols used in the manual
This symbol refers to general hazards.
CAUTION means that material damage could occur.
WARNING means that injuries or material damage or contamination could occur.
This symbol refers to biological hazards.
Observe the information contained in the instruction manual to keep yourself and your
environment safe.
This symbol refers to electrical hazards.

Thermo Scientific
Sorvall ST 8 / 8R Centrifuge |11
Technical Specifications
Technical Data
Sorvall ST 8
Environmental Conditions Use in interior spaces
Altitudes of up to 2000 m above sea level
Max. relative humidity 80% up to 31 °C;
decreasing linearly to 50% relative humidity at 40 °C
Environmental Conditions
during Storage and Shipping
Temperature: -10 °C to +50 °C
Humidity: 15% to 85%
Permissible Ambient Temperature
during Operation +2 °C to +35 °C
Heat Dissipation1100 V, 50 / 60 Hz 120 V, 60 Hz 230 V, 50 / 60 Hz
0.31 kWh/h; 1060 Btu/h;
1120 kJ/h
0.31 kWh/h; 1060 Btu/h;
1120 kJ/h
0.31 kWh/h; 1060 Btu/h;
1120 kJ/h
Overvoltage Category II
Pollution Degree 2
IP 20
Running Time 99 h 59 min 50 sec, hold
Maximum Speed nmax 16000 rpm
Minimum Speed nmin 300 rpm
Maximum RCF Value at nmax 24328x g
Noise Level at Maximum Speed1< 58 dB (A) (measured with a TX-150 rotor); < 61 dB (A) (measured with a MicroClick 24x2 rotor)
Maximum Kinetic Energy 8.12 kJ
Dimensions
Height (open door / closed door) 660 mm / 310 mm
370 mm
490 mm
Width
Depth
Weight235 kg
1Front Side Measurement, 1 m in front of the instrument at 1.6 m height
2Without Rotor
1

Chapter 1 | Technical Data
12 |Sorvall ST 8 / 8R Centrifuge
Thermo Scientific
Sorvall ST 8R
Environmental Conditions Use in interior spaces
Altitudes of up to 3000 m above sea level
Max. relative humidity 80% up to 31 °C;
decreasing linearly to 50% relative humidity at 40 °C
Environmental Conditions
during Storage and Shipping
Temperature: -10 °C to +50 °C
Humidity: 15% to 85%
Permissible Ambient
Temperature during Operation +2 °C to +35 °C
Heat Dissipation1100 V, 50 / 60 Hz 120 V, 60 Hz 230 V, 50 / 60 Hz
0.35 kWh/h; 1170 Btu/h;
1140 kJ/h
0.35 kWh/h; 1170
Btu/h; 1140 kJ/h
0.35 kWh/h; 1170
Btu/h; 1140 kJ/h
Overvoltage Category II
Pollution Degree 2
IP 20
Running Time 99 h 59 min 50 sec, hold
Maximum Speed nmax 17850 rpm
Minimum Speed nmin 300 rpm
Maximum RCF Value at nmax 30279 x g
Noise Level at Maximum Speed1< 56 dB (A) (measured with a TX-150 rotor)
Maximum Kinetic Energy 10.1 kJ
Temperature Setting Range -10 °C to +40 °C
Dimensions
Height (open door / closed door) 700 mm / 320 mm
460 mm
670 mm
Width
Depth
Weight274 kg
1Front Side Measurement, 1 m in front of the instrument at 1.6 m height
2Without Rotor

Directives, Standards and Guidelines | Chapter 1
Thermo Scientific
Sorvall ST 8 / 8R Centrifuge |13
Directives, Standards and Guidelines
Region Directive Standard
Europe
230 V, 50 / 60 Hz
2006/95/EC
Low Voltage (LVD)
2006/42/EC
Machinery (MD)
2004/108/EC
Electromagnetic Compatibility (EMC)
98/79/EC
In-vitro-Diagnostika (IvD)
2002/96/EC
Waste Electrical and Electronic
Equipment (WEEE)
2011/65/EC
Restriction of the use of certain
hazardous substances in electrical
and electronic equipment (RoHS)
1907/2006
Registration, Evaluation, Authorisation,
and Restriction of Chemicals including
substances of very high concern,
SVHC (REACH)
EN 61010-1, 2nd Edition
EN 61010-2-020, 2nd Edition
EN 61010-2-101, 2nd Edition
EN 61326-1 Class B
EN 61326-2-6
EN ISO 13485
North America (USA & Canada)
230 V, 60 Hz
120 V, 60 Hz
FDA Device Class 1
Product code JQC: centrifuges for
clinical use
CAN/CSA-C22.2 No. 61010-1-04
UL Std. No. 61010-1, 2nd Edition)
CAN/CSA-C22.2 No. 61010-2-020-
09-Part 2-020
IEC 61010-2-020, 2nd Edition
IEC 61010-2-101, 2nd Edition
EN ISO 13485
Japan
100 V, 50 Hz
100 V, 60 Hz
China
230 V, 50 / 60 Hz
IEC 61010-1, 2nd Edition
IEC 61010-2-020, 2nd Edition
IEC 61010-2-101, 2nd Edition
EN 61326-1 Class B
EN 61326-2-6
EN ISO 13485

Chapter 1 | Mains Supply
14 |Sorvall ST 8 / 8R Centrifuge
Thermo Scientific
Mains Supply
The following table contains an overview of the electrical connection data. This data is to be taken into consideration, when
selecting the mains connection socket.
Unit Sorvall ST 8 Sorvall ST 8R
Article No. 75007202 75007200 75007201 75007205 75007203 75007204
Mains Voltage 100 V 120 V 230 V 100 V 120 V 230 V
Frequency 50 / 60 Hz 60 Hz 50 / 60 Hz 50 / 60 Hz 60 Hz 50 / 60 Hz
Rated Current 5.5 A 5 A 2 A 10 A 8 A 4 A
Power Consumption 310 W 310 W 310 W 850 W 700 W 750 W
Equipment Fuse 10 AT 10 AT 5 AT 15 AT 15 AT 15 AT
Building Fuse 15 AT 15 AT 16 AT 15 AT 15 AT 16 AT
Rotor Selection
The Thermo Scientific Sorvall ST 8 / 8R Centrifuge is supplied without a rotor. Various rotors are available. If not stated
otherwise the rotors can be used in the Thermo Scientific Sorvall ST 8 centrifuge as also in the Thermo Scientific Sorvall
ST 8R centrifuge.
Part No. Thermo Scientific Rotors
75005701 TX-150 swinging bucket rotor
75005702 TX-150 round buckets
75005703 TX-150 50mL conical buckets
75005704 TX100S clinical swinging bucket rotor with sealed carriers
75005705 TX100 clinical swinging bucket rotor with carriers
75005706 M10 microplate swinging bucket rotor
75005723 M10 buckets
75005721 M10 sealed buckets
75005600 MT12 microtube swinging bucket rotor
75005709 HIGHConic III fixed angle rotor
75003623 CLINIConic fixed angle rotor
75005715 MicroClick 24x2 microtube rotor
75005719 MicroClick 30x2 microtube rotor
75005720 8x8 PCR Strip rotor
75005733 Hematocrit rotor
75003694 8 x 50 mL Individually Sealed rotor (only usable in the Sorvall ST 8R)
75005765 MicroClick 18x5 microtube rotor (only usable in the Sorvall ST 8R)
For further details on rotor use and safety, additional accessories, adapters and spare parts, please refer to the rotor
manual.
For more information visit our website at http://www.thermoscientific.com/centrifuges

Thermo Scientific
Sorvall ST 8 / 8R Centrifuge |15
Transport and Set Up
Before Setting Up
1. Check the centrifuge and the packaging for any shipping damage. Inform the shipping company and Thermo Fisher
Scientific immediately if any damage is discovered.
2. Remove the packaging.
3. Check, if the items supplied are complete. Ssee “Items Supplied” on page 7.
If the items supplied are incomplete, please contact Thermo Fisher Scientific.
Location
The centrifuge is only to be operated indoors.
The set-up location must fulfill the following requirements:
A safety zone of at least 30 cm must be maintained around the centrifuge.
People and hazardous substances must be kept out of this safety zone while centrifuging.
The supporting structure must be stable and free of resonance.
The supporting structure must be suitable for horizontal setup of the centrifuge.
The centrifuge is not to be exposed to heat and strong sunlight.
WARNING
UV rays reduce the stability of plastics.
Do not subject the centrifuge, rotors and plastic accessories to direct sunlight.
The set-up location must be well-ventilated at all times.
Transporting
WARNING
Always lift the centrifuge from both sides. Never lift the centrifuge by its front panel, its back panel or at its door.
Always remove the rotor before moving the centrifuge.
2

Chapter 2 | Leveling
16 |Sorvall ST 8 / 8R Centrifuge
Thermo Scientific
Due to its weight, the centrifuge should be carried by at least two people. Always lift the centrifuge at both sides.
To prevent possible injuries at least two people should lift and carry the centrifuge by holding it at the bottom from opposite
sides.
Transport the centrifuge and accessories upright within the associated packaging, if possible.
NOTICE
Store the original centrifuge packaging. Contact a shipping company or the customer service for the transport.
Always remove the rotor before moving the centrifuge. If you do not remove the rotor you might damage the
centrifuge drive or centrifuge spindle.
Leveling
CAUTION
If the centrifuge isn’t leveled, imbalances can occur and the centrifuge can be damaged.
Do not place anything under the centrifuge feet to level the centrifuge.
The centrifuge is to be placed on horizontal and level supporting structures or benching.
Hoirzontal level is to be checked when moving the centrifuge to a new location.
Mains Connection
NOTICE
Plug the centrifuge into grounded electrical sockets only.
1. Turn off the power supply switch.

Storage | Chapter 2
Thermo Scientific
Sorvall ST 8 / 8R Centrifuge |17
Power supply switch on the backside of the Sorvall ST 8 Power supply switch on the right side of the Sorvall
ST 8R
2. Check whether the cable complies with the safety standards of your country.
3. Make sure that the voltage and frequency correspond to the figures on the rating plate.
4. Establish the connection to the power supply with the connecting cable.
Storage
WARNING
When removing the centrifuge and accessories from use if necessary clean, disinfect or decontaminate the entire
system. In doubt contact the Thermo Fisher Scientific customer service.
Before storing the centrifuge and the accessories, it must be cleaned and if necessary desinfected and
decontaminated.
Centrifuge, rotors, buckets and accessories have to be thoroughly dried before storage.
Store the centrifuge in a clean, dust-free location.
Be sure to place the centrifuge on its feet.
Avoid direct sunlight.
Shipping
WARNING
Before shipping the centrifuge and accessories you have to clean and if necessary disinfect or decontaminate the
entire system. In doubt contact the Thermo Fisher Scientific customer service.
Before shipping the centrifuge please bear the following in mind:
The centrifuge must be clean and decontaminated.
The decontamination must be confirmed in a decontamination certificate. Contact customer service for more
details.