Thermo Electron C3i User manual

C3i - CR3i
Jouan 4 x 280 mL Multifunction Centrifuges
User’s Manual
Manual N°: 89003152-a

CAREFULLY READ THIS MANUAL BEFORE OPERATING YOUR INSTRUMENT.
INFORMATION CONTAINED IN THIS DOCUMENT IS THE PROPERTY OF
THERMO ELECTRON CORPORATION;
IT MAY NOT BE DUPLICATED OR DISTRIBUTED WITHOUT THE OWNER’S AUTHORIZATION.
THE VALIDITY OF THE GUARANTEE IS SUBJECT TO THE OBSERVATION OF THE INSTRUCTIONS
AND PRECAUTIONS DESCRIBED IN THIS DOCUMENT
C3i-CR3i

Revision Status
C3i-CR3i Revision Status
REV DATE AMENDED PAGES NOTES
a 07/04 Initial release

Packing List
C3i-CR3i Packing List
Item number Quantity Check
Centrifuge A14i micron - 230V 50/60 Hz
AA24.13 - Rotor 24 x 1,5/2,2 mL 1 c
89002796 User manual 1 c
64227001 Mains lead 1 c
26915005 Lid opening tool 1 c
26792017 Rotor locking nut 1 c
26792017 Rotor locking washer 1c
86004061 Rotor locking wrench 1 c
86003941 Fuse 5X20 3.15A T 250V 2 c
Item number Quantity Check
Centrifuge 1 c
User manual 1c
CE Declaration of compliance 1 c
Manual lid opening instructions 1 c
Mains cord 1 c
Toolbox :
- Rotor removal tool 1 c
- Emergency unlocking tool 1 c
- Spare fuses (C3i only) 2c
c11175770 Centrifuge C3i 230V 50/60 Hz
c11175771 Centrifuge C3i 120V 60 Hz
c11175774 Centrifuge CR3i 230V 50/60 Hz
c11175775 Centrifuge CR3i 120V 60 Hz
For missing parts call your nearest Thermo representative

C3i-CR3i Guarantee Terms
Guarantee Terms
JOUAN guarantees that this unit is free from defects in materials and workmanship when it leaves the factory,
and will replace or repair the unit if it proves defective in normal use or during service for a period of ONE YEAR
from the delivery.
Our liability under this guarantee is limited to repairing the defective unit or any part of the unit providing it is
sent, postage paid, to an authorized service center or the SAINT-HERBLAIN office.
This guarantee is invalid if the unit is incorrectly used, poorly serviced or neglected, mis-used or accidentally
damaged.
There is no explicit guarantee other than as stated above.
For further information, assistance or service :
Belgium:
Brussels
Phone [phone]
Fax. [phone]
China:
Beijing
Phone [phone]
Fax. [phone]
Shanghai
Phone [phone]
Fax. [phone]
Denmark:
Allerød
Phone [phone]
Fax. [phone]
Finland:
Vantaa
Phone [phone]
Fax. [phone]
France:
Saint-Herblain (Nantes)
Phone [phone]
Fax. [phone]
Germany:
Dreieich
Phone [phone]
Fax. [phone]
Hong Kong:
Wanchai
Phone [phone]
Fax. [phone]
India:
Navi Mumbai
Phone [phone]
Fax [phone]
Italy:
Cologno Monzese (MI)
Phone [phone]
Fax [phone]
Japan:
Yokohama City
Phone [phone]
Fax [phone]
Netherlands:
Breda
Phone [phone]
Fax. [phone]
Russia:
Moscow
Phone [phone]
Fax. [phone]
Spain:
Barcelona
Phone [phone]
Fax. [phone]
Madrid
Phone [phone]
Fax. [phone]
Sweden:
Stockholm
Phone [phone]
Fax. [phone]
UK:
Basingstoke, Hampshire
Phone [phone]
Fax. [phone]
USA / Canada:
Milford, MA
Phone +1 866-9 THERMO (984-3766)
Fax. [phone]
Winchester, VA
Phone [phone]
Fax. [phone]
Other Countries:
Europe
Phone [phone]
Fax. [phone]
Nordic Countries
Phone [phone]
Fax. [phone]
Outside Europe
Phone [phone]
Fax. [phone]
Website : www.thermo.com

1Use and Function . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1
1.1. User Manual . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1
1.2. Warning Symbols . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1
1.3. Description . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 1
1.4. Refrigeration and Heating Systems . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2
1.5. Drive System . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2
1.6. Safety Interlock System . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2
1.7. Imbalance Detection System . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3
1.8. Relative Centrifugal Force . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3
2 Specifications . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5
2.1. Dimensions and Weight . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5
2.2. Electrical Specifications . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5
2.3. Performance . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5
3 Installation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7
3.1. Environmental Conditions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7
3.2. Unpacking . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7
3.3. Positioning . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7
3.4. Mains Supply . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8
3.5. Lid Opening and Rotor Checking . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8
3.6. Manual Lid Unlocking Procedure . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9
3.7. Performance and Accessories . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 10
4Instructions for Use . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 13
4.1. Controls and Indicators . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 13
4.2. Display Screens . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 14
4.3. Control Panel Functions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 14
4.4. Control Panel Indicators . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 15
4.5. Description of Certain Events with Respect to the Control System . . . . . . . . . . . . . . . . . . . . 16
4.6. Preparing the First Run of the Day . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 16
4.7. Sample Loading . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 17
5 Hazards, Precautions and Limitations of Use . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 19
5.1. IEC 1010-2-020 . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 19
5.2. Cautions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 19
5.3. Speed Control . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 19
5.4. Operational Limitations . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 20
5.5. Aerosol Risks . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 20
6Service and Maintenance . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 21
6.1. Periodic Cleaning . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 21
6.2. Contamination Hazards . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 21
6.3. Disinfection . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 21
6.4. Radioactive Decontamination . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 22
6.5. Power Supply Circuit Breaker . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 22
6.6. Rotor Removal . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 22
6.7. Trunnion Lubrication . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 23
6.8. Chemical Compatibility Table for Rotors, Tubes and Accessories . . . . . . . . . . . . . . . . . . . . . 24
7 Disposal of Product . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 29
Contents
C3i-CR3i Contents

1.1. User Manual
The user manual is part of the centrifuge, and contains important information for your safety and for the
best use of the equipment
Always keep the manual close to the appliance and in a safe place, so that it is always available. Thermo
strongly recommends that all users read this manual carefully.
1.2. Warning Symbols
The following symbols are provided to help the operator take advantage of the protection afforded by the
equipment and to warn of potential danger.
IOn the main switch, allows centrifuge to operate.
OOn the main switch, disconnects the centrifuge from the main power supply.
Only authorized personnel can touch the parts close to this symbol and, in any case, only after
switching off the main power supply. Coming in contact with high voltages could cause severe
injuries.
In this manual, this symbol means that you will find important information for safety, which if
unobserved could result in damage to the appliance and/or harm to the operator.
In this manual, this symbol means that you will find important information about minimizing
biological risk: if unobserved the result could be harm to the operator.
1.3. Description
The C3i and CR3i centrifuges are designed for laboratory use.
They will separate the components of fluids into layers of varying density by subjecting them to high forces.
Centrifugal force provided by the centrifuge can also be used to drive solvents and low molecular weight
solutes through the membrane of a filtering device. Retained macrosolutes will therefore be found above
the membrane.
Relative Centrifugal Force (RCF) generated by a rotor is directly proportional to its sedimentation useful
radius and to the value of its speed squared.
A control system permits the user, through an extremely intuitive control panel, to set and control the
speed, the g-force, the temperature (on the thermostated and refrigerated versions) and the run time, as well
as to view different messages and warnings.
1 Use and Function
C3i-CR3i Use and Function
1

1.4. Refrigeration and Heating Systems
The C3i is a ventilated unit. A permanent air circulation reduces the heat level in the bowl.
However the temperature in the bowl can exceed 10 °C above the ambient temperature after hours of
continuous operation.
The CR3i is equipped with a powerful refrigeration system that allows samples to be
processed at low temperature. The refrigerant, R134a, is free of CFC (Chloro-Fluoro-
Carbons) in accordance with the Montreal Protocol directions for preservation of the
ozone layer in the atmosphere.
The actual temperature in the bowl is continuously displayed on the front panel of the centrifuge. Under
certain conditions (high speed, long duration runs) the sample temperature can be higher than the bowl
temperature on the display. In this case, the user may compensate manually after empirical determination
of the temperature differential.
Condensation drain on refrigerated units
The lid should remain closed when the refrigeration system is in use to avoid
condensation.
The centrifuge has a rubber hose that allows condensation to be drained from the
bowl. The drain hose exits at the back of the machine. Near the drain hose you
can find the following label:
To remove condensation, please follow this procedure:
- Switch off the centrifuge and disconnect the power.
- Put a small basin under the hose.
- Unplug the hose.
- Drain the centrifuge bowl.
- Insert the plug back into the hose.
ATTENTION: Do not remove the plug from the drain hose during centrifugation.
1.5. Drive System
A three phase brushless motor drives the rotating equipment. The rotor is contained in a sealed, armor
plated centrifugation chamber.
The brushless motor requires no maintenance. A control system ensures the correct drive speed, which is
continuously monitored.
1.6. Safety Interlock System
The C3i and CR3i centrifuges are equipped with an interlock system that assures two basic safety features:
-The run cannot be started if the lid is not correctly closed.
- The centrifuge lid cannot be opened if the rotor is running.
The centrifuge will not operate until the lid is closed and latched in place.
The lid remains latched until the rotor stops spinning. The lid access lamp indicates when the rotor has
stopped: consequently the handle on the right hand side of the centrifuge can be used to open the lid.
C3i-CR3i Use and Function
2

C3i-CR3i Use and Function
3
If a power failure occurs, access to the samples in the centrifuge is possible via a manual lid opening
procedure (see appropriate paragraph). For this operation a special tool (supplied with the centrifuge) is
required.
Bypass the interlock system only under emergency conditions as the rotor could still be rotating.
1.7. Imbalance Detection System
The C3i centrifuge is equipped with a load imbalance detector.
In case of excessive imbalance, the load imbalance indicator LED is illuminated and the brake is applied
immediately. The rotor will be decelerated to rest in few seconds.
As soon as the motor stops, open the centrifuge and redistribute the samples to produce an equal weight on
diametrically opposite sides.
If the message persists, despite your efforts to balance the load, call your Thermo Service representative.
Note : Imbalance tolerance depends upon the rotor in use. The centrifuge will tolerate 10 gr. of imbalance
with the 4 x 280 mL rotor equipped with standard buckets. Carefully balance the sample load to avoid
actuating the imbalance detection system.
1.8. Relative Centrifugal Force
Relative Centrifugal Force (RCF), at the circumference of a rotor and bucket combination, is directly
proportional to the speed (r.p.m.) and radius of the rotor. Therefore, a greater r.p.m. and/or a larger radius
produces a greater RCF and improved faster separation of substances. The centrifuge control system carries
out and displays the results of all calculations related to speed, radius and RCF.
Note : The value introduced for the radius can be adjusted to allow for position within the tube such as at a
boundary. Maximum radii are quoted in the specifications tables. Use of improper radius will adjust the
speed setting, automatically applying the wrong RCF.

C3i-CR3i Use and Function
4
Legend : R = radius (in millimetres)
N = speed (in r.p.m.) ÷ 1000
RCF = gravitational acceleration ‘g’
M+ = add to memory
MR = memory recall
NOTE : To calculate actual results, press the keys on a pocket calculator in the order shown.
Primary calculations Key sequence (not valid for CASIO, HP)
RCF (x g) = 1.118 R N2N x = x 1.118 x R =
Speed (r.p.m.) = 946 RCF RCF ÷ R = √x 946 =
R
Radius (mm) = RCF N x = x 1.118 = M+ RCF ÷ MR =
1.118 N2
Transformations Key sequence
To determine actual ‘g’ achieved at a different speed :
RCF2 = RCF1
(
N2
)
2N2 ÷ N1 = x = x RCF1 =
N1
To determine actual speed required to achieve a different ‘g’ at the same radius :
N2 = N1 RCF2 RCF2 ÷ RCF1 = √x N1 =
RCF1
To determine actual speed required to achieve the same ‘g’ at a different radius :
N2 = N1 R1 R1 ÷R2 = √x N1 =
R2
Centrifugation formulae

2.1. Dimensions and Weight
Dimensions (H x W x D) C3i : 372 x 400 x 502 mm CR3i : 375 x 575 x 605 mm
Packed (H x W x D) C3i : 600 x 610 x 540 mm CR3i : 615 x 757 x 738 mm
Weight - uncrated / crated C3i : 40 kg / 52 kg CR3i : 72 kg / 85 kg
2.2. Electrical Specifications
Max power C3i : 500 W CR3i : 800 W
Average power C3i : 350 W CR3i : 550 W
Refrigeration CR3i : 235 W
2.3. Performance
Max speed Swing-out : 4 000 rpm (C3i) Angle : 14 000 rpm
Swing-out : 4 100 rpm (CR3i)
Max RCF Swing-out : 3 934 x g (C3i) Angle : 18 407 x g
Swing-out : 3 082 x g (CR3i)
Max capacity Swing-out : 4 x 280 ml Angle : 6 x 100 ml
Microprocessor controlled
Display High visibility digital display
Memory size 5 programs, direct access
Program protection Recall key lock
Speed Range 500 to 14 000 rpm
Step 10 -100 rpm
Accuracy ± 20 rpm
Timer Range 30 sec to 99 min + hold position
Step 30 sec to 1 min
Acceleration rates 5
Braking rates 5
Temperature (CR3i) Range -9°C to +40°C
Step 1°C
Accuracy ± 1.5°C
Typical performance 4°C at 4 000 rpm, (4 x 280 ml swing-out)
1°C at 14 000 rpm, (20 x 1.5 ml angle)
Maximum density 1.2 g/cm3
Maximum energy 14 400 J
2 Specifications
C3i-CR3i Specifications
5

C3i-CR3i Specifications
6

3.1. Environmental Conditions
General conditions accepted for centrifuge transport and storage are:
- Ambient temperature -20°C to +50°C.
- Relative humidity up to 90%.
General conditions accepted for operating the centrifuge safely are:
- Indoor use.
- Temperature: 5 °C to 40 °C.
- Maximum relative humidity of 85%.
- Maximum altitude: 2000 m
- Installation category: II
- Polution degree: 2
3.2. Unpacking
Due to the weight of the machine, all lifting and transporting must be carried out using proper handling
equipment (i.e. fork lift trolley) that complies with current regulations, and by people having undergone the
necessary training.
Thermo strongly recommends that all operators comply with the local laws and regulations on
safety and health in the workplace.
The machine must be supported from underneath. If it has to be transported without its pallet, for
example on a staircase, professional handling assistance is required.
-Unpack the centrifuge, carefully removing any possible accessories and the material supplied for ordinary
operations and maintenance.
- Check the contents of the package using the packing list provided above.
- Keep the packaging until the centrifuge has been tested and found fully functional.
3.3. Positioning
The machine must be installed in a dust and corrosion free room.
Leave a 30 cm / 12 in. space free on each side of and behind the machine for safety reasons,
proper ventilation and maximum cooling performances.
Place the centrifuge on a bench top, which must be rigid, horizontal and sufficiently strong to support the
centrifuge’s weight and small vibrations.
3 Installation
C3i-CR3i Installation
7

For your safety, check that mains wiring is effectively grounded.
Thermo declines all responsibility for any damages due to non-grounding of the machine.
Remember that in order to respect the electrical safety standards related to protection against indirect
contact, the supply of power to the instrument must be via a power socket fitted with a protection device
ensuring automatic cut-off in the case of an insulation fault. A supply fitted with a circuit breaker of the
correct rating complies with this requirement.
3.5. Lid Opening and Rotor Checking
Ensure that the centrifuge has been switched ON. Pull the handle on the centrifuge right side towards the
front of the unit : the lid is automatically unlocked and opens.
In the case of a mains power outage, opening of the lid is prevented by the lid lock safety device. It is
recommended to wait for the mains power to be switched back on so that this safety device enables the lid
to be unlocked (refer to 3.6 for manual lid opening).
Carefully clean the inside of the centrifugation chamber removing any packing residues.
In fact, due to air turbulence, solid particles accidentally left in the centrifugation chamber create
excessive wear of the chamber itself and of the outer rotating equipment surface.
To install the rotor:
Carefully lower the rotor onto the drive shaft. Press down on the rotor until a click is heard. Try to lift the
rotor. When correctly placed, it will not move, being automatically locked onto the drive.
3.4. Mains Supply
Check mains and frequency: they must correspond to the values shown on the instrument identification
label.
C3i-CR3i Installation
8
Cat. No. Content Voltage Frequency Type
11175770 C3i 230V ± 10% 50/60 Hz 10 A single phase + ground
11175774 CR3i
11175771 C3i 120V +5% -10% 60 Hz 16 A single phase + ground
11175775 CR3i
It is not necessary to orientate the rotor relative to the drive shaft in order to achieve locking. The AUTO-
LOCK rotor mounting system allows rotors to be placed in any orientation.
1)
“clic”
2)

C3i-CR3i Installation
9
3.6. Manual Lid Unlocking Procedure
In the event of mains non-availability or power failure, opening of the lid is prevented by the lid locking
safety device. It is recommended that the user waits for the mains to be switched back on so that this safety
device enables the lid to be unlocked.
Manual lid unlocking must only be done by someone informed of the possible danger and of the necessary
precautions.
Rotating parts are a risk as they could come in contact with the user or be ejected. There is
particularly high risk of injury if:
- The user attempts to manually stop the rotor
- Any object falls inside the centrifugation chamber while the rotor is running.
Manual lid unlocking may be necessary under a very limited number of conditions, such as the urgent
recovery of critical samples that could be damaged if left in the centrifuge rotor.
In this case the lid can be opened by using the special tool supplied with the centrifuge.
Always set the power switch to the OFF position before performing this manual procedure, even in
the case of a mains power outage.
Should the emergency occur due to power failure during centrifugation wait at least 10 minutes for the end
of the rotor rotation. In spite of the absence of noise, the rotor could still be rotating when you need to
open the lid manually. Upon opening, lift the lid by hand and observe the rotor (be careful in this
operation): if it is still rotating, close the lid and wait.
The protection sticker must be replaced after it has been pierced (item N°85241772).
!!
En cas de nécessité, pour le déverrouillage manuel du
couvercle, attendre 10 min. ou l’arrêt du rotor avant
d’utiliser l’outil spécifique fourni à cet effet.
Cette étiquette, après avoir été percée, doit être
changée.
If it is necessary to open the lid manually, wait 10 min. or
until the rotor has stopped rotating, before using the lid
opening tool provided.
This label must be changed once it has been pierced.
Percer ici avec l'outil
Pierce here
withtool
85241772
!!
En cas de nécessité, pour le déverrouillage manuel du
couvercle, attendre 10 min. ou l’arrêt du rotor avant
d’utiliser l’outil spécifique fourni à cet effet.
Cette étiquette, après avoir été percée, doit être
changée.
If it is necessary to open the lid manually, wait 10 min. or
until the rotor has stopped rotating, before using the lid
opening tool provided.
This label must be changed once it has been pierced.
Percer ici avec l'outil
Pierce here
withtool
85241772
While pressing up with your finger, to press
down with the unlocking tool, pull the lid
lever toward you: the lid opens upwards.
Insert the unlocking tool horizontally into the
hole on the right hand side of the instrument.

C3i-CR3i Installation
10
3.7. Performance and Accessories
NOTE : The performance figures indicated below are those of the rotors. Their use at these speeds
necessitates that the sample containers can support the corresponding forces.
The maximum speed attainable by a given rotor depends upon the model of centrifuge which is driving it.
Rotor selection table
Catalogue Rotor Description Capacity (each) Tube dim. Rad. Max speed Max Angle
number mm mm r.p.m RCF (x g) deg.
11175710 T40 Swing-out rotor 4 x 280 ml Ø 77 164 C3i : 4000 C3i : 2934 90
CR3i : 4100 CR3i : 3082
11175711 Set of 4 buckets 280 ml nominal Ø 77 4100 3082
11175712 4 sealing lids
11175763 Set of 4 buckets 15 x 5/7 ml Ø 13.5 165 4100 3100
11175752 Set of 4 buckets 4 x 50 ml con* 33 x 120 169 4100 3176
11175759 Set of 4 buckets 3x50ml con self-standing* 33 x 120 169 4100 3176
11175713 Set of 4 inserts 1 x 280 ml 64 x 113 161 4100 3026
11175714 Set of 4 inserts 1 x 250 ml flat 61 x 112 161 4100 3026
11175715 Set of 4 inserts 1 x 200 ml flat 55 x 110 156 4100 2932
11175716 Set of 4 inserts 1 x 175 ml conical 60 x 120 178 4100 2969
11175717 Set of 4 inserts 1 x 135 ml 43 x 121 161 4100 3025
11175718 Set of 4 inserts 1 x 110 ml 40 x 122 158 4100 2969
11175719 Set of 4 inserts 1 x 100 ml 38 x 123 161 4100 3026
11175720 Set of 4 inserts 2 x 65 ml 34 x 104 156 4100 2932
11175721 Set of 4 inserts 4 x 50 ml 28.5 x 106 132 4100 2481
11175722 Set of 4 inserts 2 x 50 ml conical 29 x 114 134 4100 2518
11175723 Set of 4 inserts 5 x 38 ml 25.5 x 102 157 4100 2950
11175724 Set of 4 inserts 5 x 25 ml 24 x 104 155 4100 2917
11175725 Set of 4 inserts 4 x 25 ml Corex 24 x 107 155 4100 2913
11175726 Set of 4 inserts 9 x 16 ml 18 x 107 160 4100 3007
11175727 Set of 4 inserts 12 x 15 ml Corex/10 ml vacu 17 x 107 160 4100 3007
11175729 Set of 4 inserts 7 x 15 ml conical 17 x 108 161 4100 3026
11175730 Set of 4 inserts 4 x 15 ml conical 17 x 119 163 4100 3063
11175731 Set of 4 inserts 5 x 15 ml conical* 17 x 116 163 4100 3063
11175747 Set of 4 inserts 4 x 14 ml Corning 15.5 x 121 163 4100 3063
11175732 Set of 4 inserts 12 x 13 ml 16 x 115 160 4100 3007
11175728 Set of 4 inserts 9 x 10 ml LP 16 x 112 160 4100 3007
11175733 Set of 4 inserts 19 x 8 ml 12 x 104 161 4100 3026
11175734 Set of 4 inserts 19 x 5 ml RIA 13 x 104 161 4100 3026
11175735 Set of 4 inserts 19 x 3 ml 11 x 106 161 4100 3026
11175736 Set of 4 inserts 13 x epp.1.5/2 ml 10 x 42 159 4100 2988
11175738 Set of 4 inserts 4 x 15 ml vacu* 16 x 124 163 4100 3063
Note : the RCF applied to tubes in T40 inserts is about 5% lower for the C3i.
* In open buckets only

C3i-CR3i Installation
11
Catalogue Rotor Description Capacity (each) Tube dim. Rad. Max speed Max Angle
number mm mm r.p.m RCF (x g) deg.
11175749 Set of 4 inserts 12 x 7 ml vacu 12.5 x 106 160 4100 3007
11175740 Set of 4 inserts 12 x 5 ml vacu 12.5 x 75 160 4100 3007
11175742 Set of 4 inserts 1 x AMICON Centripep 28 x 135 160 4100 3007
11175753 Set of 2 3 cyto samples 108 4100 2030 90
cytobuckets per cytobucket
11175750 T20 Swing-out rotor 6 x microplates 115 3000 1157 90
with carriers or 2 x blocs
11175631 2 sealed carriers 2 x 2 microplates
11174207 Rubber cushion for flexible microtitre plates
11175755 AC 15.4 Angle rotor 30 x 15 ml 17.5 x 100 135 C3i : 4000 C3i : 2415 37
CR3i : 4100 CR3i : 2537
11175756 AC 100.10 Angle rotor 6 x 100 ml 38 x 101 99 9000 8965 25
11174713 Set of 6 adaptors 1 x 50 ml 29 x 103 93 9000 8422
11174714 Set of 6 adaptors 1 x 50 ml conical 30 x 116 93 9000 8422
11174724 Set of 6 adaptors 1 x 30 ml Corex 24 x 106 93 9000 8422
11174725 Set of 6 adaptors 1 x 25 ml Corex 24 x 102 93 9000 8422
11174726 Set of 6 adaptors 1 x 15 ml Corex/16 ml 18 x 102 92 9000 8331
11174715 Set of 6 adaptors 1 x 30 - 38 ml 25.5 x 92 88 9000 7969
11174716 Set of 6 adaptors 1 x 15 ml conical 17 x 122 93 9000 8422
11174717 Set of 6 adaptors 2 x 10 ml 16 x 80 83 9000 7516
11174718 Set of 6 adaptors 4 x 1.5 - 2 ml 11 x 39 79 9000 7154
11175754 AC 50.10 Angle rotor 6 x 50 ml con./ round 29.5 x 118 112 10000 12520 40
11174606 Set of 4 adaptors 1 x 15 ml conique 17,5 x 122 112 10000 12520
11177378 Set of 4 adaptors 1 x 10 ml 16 x 80 91 10000 10174
11174599 Set of 8 adaptors 1 x 30 / 32 ml Ø 26 105 10000 11738
11175737 AC 10.12 Sealed angle rotor 10 x 10 ml 16 x 80 85 12000 13684 40
11175757 Spare sealing lid
11174607 Set of 10 adaptors 1 x 6 ml 13 x 100 80 12000 12879
11174603 Set of 10 adaptors 1 x 1.5 - 2 ml 11 x 39 64 12000 10303
11175739 AC 1.14 Angle rotor + lid 20 x 1.5 ml 11 x 39 78 14000 17092 45
11175741 AC 2.14 Sealed angle rotor 24 x 1.5 ml 11 x 39 84 14000 18407 45
41174928 Set of 20 adaptors 1 x 500 - 800 µl Ø 8
11174631 Set of 20 adaptors 1 x 200 µl PCR Ø 6.5
41174938 Set of 20 adaptors 1 x 250 / 400 / 700 µlØ6
11175743 DC 6.11 Drum rotor 6 racks 72 10000 8050 90
11174561 1 rack 10 x 1.5 - 2 ml Ø 11
11174573 1 rack 20 x 500 - 800 µl Ø 8
11174574 1 rack 20 x 700 µl Ø 6
11174563 1 rack 21 x 600 µl Ø 6
11174562 1 rack 32 x 250 - 400 µl Ø 6

C3i-CR3i Installation
12

4.1. Controls and Indicators
All the controls are located on the front panel. The front panel is an intuitive interface: no previous
operational knowledge is necessary; every parameter can be set by pressing the cursor key continuously,
from the minimum to the maximum and viceversa. All parameters can be accessed and changed both
during operation and while the machine is at rest.
Buttons
1 - 5 Program Keys
6 Speed/RCF Toggle
7 Time/Radius Toggle
8 Acceleration rate set
9 Deceleration rate set
10 Stop Run
11 Pulse Run
12 Start Run
13 Temperature set (R only)
Display Screens
15 Upper Screen: speed / RCF
16 Middle Screen: time / raduis
17 Lower Screen: accel. / decel. rates /
temp. (R models)
LED Lights
1a Program 1 Indicator
2a Program 2 Indicator
3a Program 3 Indicator
4a Program 4 Indicator
5a Program 5 Indicator
8a Acceleration Indicator
9a Deceleration Indicator
13 Imbalance Indicator
14 Lid Opening allowed Indicator
18 Speed Indicator
19 RCF Indicator
20 Time Indicator
21 Radius Input Indicator
7 6 19 18 20 21
9a 9 17 13 11 12 13 14
4 Instructions for Use
C3i-CR3i Instructions for Use
13
1a
1
2a
2
3a
3
4a
4
5a
5
15
16
8a
8
10

C3i-CR3i Instructions for Use
14
4.3. Control Panel Functions
4.2. Display Screens
Screen
Upper screen
(15)
Middle screen
(16)
Lower screen
(17) on the C3i
Lower screen
(17) on the R
units
Description
This screen displays the set speed (rpm) or RCF (x g) for the given program
when the centrifuge is at rest, depending on which parameter is selected
During a run, this screen displays actual speed or RCF.
This screen displays either the set run time or the centrifugal radius when the
centrifuge is at rest, depending on which parameter is selected.
During a run, this display shows only time remaining (timed runs) or time
elapsed (during deceleration, ‘hold’ runs and ‘pulse’ runs).
This screen displays either the acceleration or the deceleration profile selected
for the given program. The value of the acceleration profile is displayed either
during acceleration or when the acceleration set button (8) is pressed. The
value of the deceleration profile is displayed either during deceleration or
when the deceleration set button (9) is pressed.
This screen displays either the acceleration or the deceleration profile selected
for the given program. The value of the acceleration profile is displayed either
during acceleration or when the acceleration set button (8) is pressed. The
value of the deceleration profile is displayed either during deceleration or
when the deceleration set button (9) is pressed.
In addition, it shows the temperature in the bowl of the centrifuge. When the
centrifuge is at rest, the set bowl temperature is displayed (°C). During a run,
the actual bowl temperature is displayed (°C).
Function
Start cycle
Stop cycle,
immediate
Pulse
Set speed
Set RCF
Set run time
Keystroke sequence and description
Press the start button (12).
Press the stop button (10)
Press the pulse button (11) and hold. The centrifuge will accelerate the rotor
to the speed setting of the active program. When the button is released, the
rotor will be decelerated to rest.
When the LED next to ‘RPM’ (18) is illuminated, the upper display shows
speed values. To increase/decrease the speed values, press on the
corresponding up/down arrows.
When the LED next to ‘G’ (19) is illuminated, the upper display shows RCF
values. To increase/decrease the RCF values, press on the corresponding
up/down arrows.
When using RCF as a control/display value, make sure the correct centrifugal
radius is entered into the control system (see below). If an incorrect radius
value is in the control system, the RCF value displayed will not be accurate.
When the LED next to ‘TIME’ (20) is illuminated, the middle display shows
time values. To increase/decrease the run time values, press on the
corresponding up/down arrows.