Thermo Savant UVS400 User manual

A Thermo Electron business
UVS400/UVS400A
Universal Vacuum System
INSTRUCTION MANUAL

Table of Contents
Section Section Title — Description Page
1.0 Description 3
Section Section Title — Installation Page
2.0 Installation 4
Unpacking 4
Inspection 4
Set-up 4
2.1 Preparing the Vacuum System for use 5
2.2 Connection to Evaporation/Drying Equipment 6
Section Section Title — Operation Page
3.0 Operation 6
3.1 Emptying the Glass Condensation Flask 7-8
3.2 Optional Traps 8
Section Section Title — Specifications Page
4.0 Specifications 9
Section Section Title — Accessories Page
5.0 Accessories 9
Section Section Title — Warranty Page
6.0 Warranty and Liability 10
Disclaimer:
All statements, information and data given herein are believed to be accurate and reliable but are presented without guarantee, or responsibility of any kind,
expressed or implied. Statements or suggestions concerning possible use of our products are made without representation that any such use is free of patent
infringement and are not recommendations to infringe any patent. The user should not assume that all safety measures are indicated, or that other measures may
not be required.
VaporNet®, SpeedVac®, and CryoCool®are registered trademarks of Thermo Savant.
TEFLON®is a registered trademark of E.I. Dupont de Nemour & Co.

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1.0 DESCRIPTION
Thermo Savant’s Universal Vacuum System (UVS400/UVS400A) is a multipurpose vac-
uum source and solvent recovery system, combined in a single, integrated unit (Figure 1).
The system includes a refrigerated vapor trap, and oil-free vacuum pump; the UVS400A
version (220 V/60 Hz or 230 V/50 Hz) also includes a VaporNet®controller. Together,
these elements form a low-maintenance vacuum system that offers improved reliability
over those based on oil-filled vacuum pumps, which require frequent maintenance and
can easily be damaged by contaminating liquids.
The Universal Vacuum System is ideal for applications that evaporate solvents from sam-
ples or substances. It is used with Thermo Savant’s SpeedVac®vacuum concentrator, Gel
Dryer, or other vacuum-dependent equipment such as vacuum ovens or rotary evapora-
tors. The vapor trapping efficiency and thermal capacity of the UVS400/UVS400A pro-
vides evaporation rates equivalent to or better than component systems relying on main-
tenance-intensive rotary vane oil vacuum pumps. At the same time, the
UVS400/UVS400A is the most energy efficient and environmentally safe vacuum system
available, providing complete recovery of evaporated solvents.
Three major elements are incorporated within the UVS400/UVS400A. The refrigerated
vapor trap features a Glass Condensation Flask that collects condensing solvent vapors
generated by the attached equipment. Recovered condensate can then be safely handled
for proper disposal or recycling. The oil-free vacuum pump eliminates frequent mainte-
nance required by oil-filled pumps, and is corrosion-resistant for long life.
Features:
•-50 ˚C operating temperature, 4-liter vapor trap, CFC-free refrigerant
• Wide-mouth Glass Condensation Flask (GCF400)
• 34.6 cm (13.6 in) of bench width used
• VaporNet controller (UVS400A, version only)
•TEFLON®coated oil-free vacuum pump, 36 l/min displacement
(@ 60 Hz), 10 torr ultimate vacuum
•Automatic vacuum start sequence
•Optional traps for ammonia vapors, acids, and volatile
radioactivity/organics
Inlet port
Refrigerated
Vapor Trap
Stainless Steel
Refrigerated Chamber
Oil-Free
Vacuum Pump
Solvent Exhaust Port
Flask Cover
Insulating Foam Ring
Glass Condensation Flask
Vacuum On/Off switch
Figure 1. Universal Vacuum System UVS400

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2.0 INSTALLATION
Unpacking. Carefully remove the instrument from its shipping carton. Lift and carry
with two people, holding securely underneath with both hands. Use proper lifting tech-
nique (lift with the legs, not the back) to avoid personal injury. Compare the packing list
to the box contents. If there is a discrepancy, call Thermo Savant at 1-800-634-8886 or
631-244-2929, (fax) 631-244-0606 or contact your distributor.
Inspection. Inspect the unit for any damage that may have occurred during shipment.
Should there be damage, report it to the carrier and contact Thermo Savant or your dis-
tributor immediately. Make sure the carrier inspects the damage and leaves an inspec-
tion report. Register any claims for shipping damage against the carrier or his agent.
Save the shipping carton and all packing materials in the event a return is necessary.
Set-up. To assure safe operation and best results, read this manual in its entirety
before operating the UVS400/UVS400A Universal Vacuum System.
•Place the Universal Vacuum System on a stable, level laboratory counter or
heavy duty cart near a power outlet of the required voltage. The bench depth
should be at least 66 cm (26 inches). The outlet must have a rating of at least
10 A for 230 V operation. The system draws high current when first switched
on; therefore, other high-power equipment, or equipment that will be affected by
a momentary drop in power, should not be placed on the same circuit. The
UVS400 is air-cooled, requiring at least 10 cm (4 inches) clearance for ambient
air circulation. The ambient temperature must not exceed 32 ˚C (90 ˚F) for
optimum function.
Warning: Before connecting the UVS400/UVS400A to an outlet, check
voltage, frequency, and amperage to be sure they match
the power requirements indicated on the label on the right
side of the instrument (115 V/60 Hz, 6 A; 230 V/50 Hz, 3 A).
If there are questions, please consult an electrician.
•As a safety feature, units are equipped with a three-prong grounded plug that fits
a grounding-type power outlet. Consult an electrician to replace outlet if neces-
sary. Do not defeat this safety feature by modifying the plug.
•This unit is “FOR INDOOR USE ONLY”. Avoid operating in areas of excessive
humidity or extremes of temperature.

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2.1 PREPARING THE VACUUM SYSTEM FOR USE
CryoCool.
Prepare the vapor trap by dispensing the correct level of CryoCool®Heat Transfer
Fluid (SCC1); available separately. Pour 800 ml of CryoCool into the stainless steel
trap chamber. A line scribed in the wall of the stainless steel chamber indicates the
minimum appropriate CryoCool level (with glass flask not yet inserted). When replen-
ishing CryoCool, add more fluid until the level reaches the scribed line.
Glass Condensation Flask (GCF400).
The GCF400 is a wide-mouth flask which facilitates removal of condensed solvent. A
rubber Flask Cover (FC400) fits over the top, and tubing attaches to this by means of
fittings pushed into molded ports on the rubber cover. This forms the vacuum connec-
tion. An insulating foam cover fits over the flask to seal in the cold and seal out atmos-
pheric condensation from the refrigerated chamber.
Place a clean, dry Glass Condensation Flask into the stainless steel chamber. Fit the
insulating cover (white foam ring) over the flask. Seal flask mouth with the black rubber
Flask Cover. Press down flask and insulating cover so that the level of CryoCool®rises
around the flask, and so that the flask, its cover, and insulation are seated to operating
position. This will fully seal the chamber. Withdraw the flask again and verify that the
level of CryoCool came to within about a half-inch (12–15 mm) from the top (shoulder)
of the flask. If the level is low, carefully pour more CryoCool into the chamber while
holding down the flask.
Immediately wipe clean any CryoCool that spills onto the rubber chamber seal or Flask
Cover.
Muffler and Optional Trays.
Attach the muffler to the solvent vapor exhaust port on the right side of the Universal
Vacuum System. For applications requiring post-pump gas trapping, attach instead the
optional Post-Trap Assembly (ANT100) or Chemical Trap Kit (DTK120; Figure 2). The
ANT100 is used with either Ammonia Neutralizing Solution (ANS121), which neutral-
ized ammonia vapors and reduces ammonia smell in the laboratory, or NSA300TF
which neutralizes acids. The DTK120 comes with a DC120R Disposable Cartridge for
adsorbing volatile radioactivity and trace organics. It may also be fitted with a DC120A
Disposable Cartridge (available separately) for neutralizing acid vapors.

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2.2 CONNECTION TO EVAPORATION/DRYING EQUIPMENT
The UVS400 Universal Vacuum System is designed for use in single or multifunction
operation, with such instruments as the SpeedVac, Gel Dryer, Vacuum Oven, or Rotary
Evaporator. Use the tubing and fitting kit (TFK120) to arrange the components in a con-
venient, space saving configuration. If necessary, place the UVS400/UVS400A on a
shelf below the evaporation/drying components. Connect instrument(s) to the port on
the left side of the UVS400/UVS400A. Install manual bleeder valve (BV130; available
separately) in the tubing between the instrument and UVS400/UVS400A. This valve
can be used to apply vacuum to the instrument, and to bleed the system back to
atmosphere pressure at the end of a run.
3.0 OPERATION
A two-position power switch (OFF/ON) is found on the right side panel. The system
should be switched ON at least 30 minutes before using to ensure that it has reached
its proper operating temperature of -50 ˚C. It is suggested that the trap be left on at all
times.
When samples or gels are in place and the evaporation/drying component is activated,
switch the vacuum switch to ON. This two-position switch (ON/OFF) is located on the
lower right-hand side of the front panel.
VAPORNET
On the UVS400A, the two-position SOLVENT switch (VOLATILE/AQUEOUS) is located
left-most on the front panel. If volatile, low boiling solvents (bp<95 ˚C) are being evapo-
rated, activate VaporNet by switching to VOLATILEto maximize solvent recovery. If non-
volatile, high boiling solvents (bp>95 ˚C) are evaporated, select Aqueous (VaporNet off).
NOTE: With VOLATILE selected, occasional clicking will be heard from the
system. This indicates that the VaporNet Controller is functioning properly.
When the concentration process is complete, switch the UVS400/UVS400A vacuum
pump OFF. Shut off VaporNet when not in use by setting to AQUEOUS.
Figure 2. a. ANT100 Post-trap Assembly b. DTK120R Chemical Trap Kit
a. b.

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3.1 EMPTYING THE GLASS CONDENSATION FLASK
During system operation, solvent vapors from the SpeedVac or other attached instru-
ment will collect in the glass condensation flask. The flask must be regularly emptied to
keep the entire system operating at peak efficiency. If the flask is not maintained, it may
become so full that sample drying rate is adversely affected. Another consequence of a
full vessel is that solvent vapors are more likely to contaminate the oil-sealed vacuum
pump bypass the trap and be released to the atmosphere.
Empty the glass flask before it is half full. Often it is convenient and good practice to
change the flask at the end of the workday or workweek, no matter the depth of fill. For
aggressive solvents, you may wish to remove the flask at the end of each run for maxi-
mum protection of the system components.
CAUTION: The UVS400 and UVS400A reach low operating temperatures that can
cause severe damage to unprotected skin. Wear protective gloves
and clothing when removing glass flasks.
To remove the GCF400 for cleaning, bleed the system back to atmospheric pressure.
Remove rubber Flask Cover from flask, leaving tubing attached to cover. Withdraw the
flask partially from the chamber and allow CryoCool to drain briefly. Fully remove flask
and insulating foam ring. Avoid thermal shock by placing the flask on several thickness-
es of absorbent paper toweling and allow to come to room temperature. Insert a spare
GCF400, which is clean and dry, into the chamber. Cover with insulating foam ring, and
seal with rubber Flask Cover. Make sure rubber cover is seated firmly for a good vacu-
um seal. This easy system maintenance can be done in a matter of minutes; the power
(refrigeration) switch need not be shut off during this process. When the used flask has
defrosted, dispose of contents in an environmentally responsible manner. Clean and dry
flask for next use.
IMPORTANT: Check the Glass Condensation Flask before each run and
replace with clean flask if approaching half full. Immediately wipe
dry any CryoCool®Heat Transfer Fluid that falls on the rubber
seal. Be sure Flask Cover is seated firmly on the flask.
NOTE: Collected solvents may be purified for reuse or disposed of safely
according to applicable regulations.
If working with radioactive samples, test condensate for presence of
radioactivity. If found to be radioactive, dispose of as radioactive
liquid according to all applicable regulations.

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3.2 OPTIONAL TRAPS
Optional traps (described in Section 2.1, Figure 2) must be maintained for optimal sys-
tem performance. The ANT100 utilizes neutralizing solutions which exhibit a color
change when depleted. Ammonia Neutralizing Solution (ANS121) changes from pink to
blue; Neutralizing Solution for Acids (NSA300TF) changes from blue to pale yellow
when depleted. Replace solution bottle when a color change is observed. If using solid
chemical cartridge with DTK120R, the DC120A cartridge for acids turns white to blue;
change when depleted. DC120R cartridge for radioactivity/organics has no color indica-
tor; replace monthly.
3.1 EMPTYING THE GLASS CONDENSATION FLASK (cont’d)
NOTE: If the Refrigerated Vapor Trap is not needed for several weeks, you
may wish to shut it off between uses. Before switching on again,
always remove the used glass vessel and replace with a clean, dry
trap. Check the condition of the CryoCool; if a layer of water is
visible under the CryoCool, remove with a pipette. Failure to follow
these precautions may cause the glass vessel to break when the
trap returns to operating temperature.
NOTE: Periodically check Flask Cover for signs of wear or perishing, and
replace if necessary.

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4.0 SPECIFICATIONS
Vacuum Source:
Type: Oil-free diaphragm, TEFLON®coated heads,
with built in VaporNet Controller
Displacement: 36 liters/min (@60 Hz), 32 liters/ min (@50Hz)
Maximum vacuum: 29.5 in Hg (10 torr)
Refrigerated Vapor Trap:
Capacity 4 liters
OperatingTemperature: -50 ˚C
Dimensions (W x D x H): 35 x 61 x 45 cm (13.6 x 24 x 18 in)
Weight: 41 kg (90 lbs)
Power Requirements: 115 V, 50/ 60 Hz, 6A
230 V, 50 Hz, 3 A
5.0 ACCESSORIES
ANT100 Post-trap Assembly
ANS121/4 Ammonia neutralizing solution, (package or 4 bottles)
DTK120R Chemical Trap Kit for radioactive samples
DC120A Disposable cartridge for acid vapor neutralization
DC120R Disposable cartridge for radiolabeled vapor adsorption
FC400 Flask Cover (black rubber)
GCF400 Glass Condensation Flask, 4 liters
BV130 Bleeder Valve
SCC1 CryoCool®Heat Transfer Fluid (1 liter)
DVG50 Digital Vacuum Gauge 0-50 Torr < 1 millitorr
CC120/DX Deluxe Convenience Cart
NSA300TF/4 Neutralizing Solution for acid (pack of 4 bottles)

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6.0 WARRANTY AND LIABILITY STATEMENTS
All Thermo Savant products (excluding glassware) are warranted against defects in mate-
rial and workmanship for one year after the date of delivery to the original purchaser.
Thermo Savant’s warranty is limited to defective materials and workmanship, and does
not cover incidental or consequential damages.
Thermo Savant will repair free of charge any apparatus covered by this warranty. If a new
component fails to work, Thermo Savant will replace it, absorb all charges, and continue
the one-year warranty period. Warranty work is subject to our inspection of the unit. No
instruments, equipment or accessories will be accepted without a Return Material
Authorization (RMA) number issued by Thermo Savant. Cost of shipping the unit are not
covered under warranty. The warranty obliges you to follow the precautions in this manu-
al.
When returning apparatus that may contain hazardous material, you must pack and label
them following U.S. Department of Transportation (DOT) regulation applying to trans-
portation of hazardous materials. Your shipping documents must also meet DOT regula-
tions. All returned units must be decontaminated and free of radioactivity.
Use of this equipment in manner other than those specified in this manual may jeopard-
ize personal safety. Under no circumstances shall Thermo Savant be liable for damages
due to the improper handling, abuse, or unauthorized repair of its products. Thermo
Savant assumes no liability, express or implied, for use of this equipment.

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145-3001-00 Rev.A
© 2001 Thermo Savant
100 Colin Drive • Holbrook, NY 11741-4306 USA
Tel: [phone] • 1-800-634-8886 • Fax: [phone]
www.thermosavant.com • E-mail: [email protected]
A Thermo Electron business