Thermo Scientific 500 User manual

Thermo Scientific
TriPlus 500
User Guide
Headspace Sampler
P/N 31716106 Revision A January 2019

© 2019 Thermo Fisher Scientific Inc. All rights reserved.
Thermo Scientific™ TriPlus™ 500 Headspace Sampler and TRACE™ 1300 Series GC (TRACE™ 1300 GC,
TRACE™ 1310 and TRACE™ 1300E) are trademarks of Thermo Fisher Scientific; VICI ® is a registered mark
of the Valco Instrument Co. Inc., and VICI AG International. All other trademarks are the property of
Thermo Fisher Scientific and its subsidiaries.
Published by Thermo Fisher Scientific S.p.A., Strada Rivoltana 20090 Rodano-Milan, Italy
Thermo Fisher Scientific Inc. provides this document to its customers with a product purchase to use in the
product operation. This document is copyright protected and any reproduction of the whole or any part of this
document is strictly prohibited, except with the written authorization of Thermo Fisher Scientific Inc.
The contents of this document are subject to change without notice. All technical information in this
document is for reference purposes only. System configurations and specifications in this document supersede
all previous information received by the purchaser.
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document shall in no way govern or modify any Terms and Conditions of Sale, which Terms and Conditions of
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Release history:
Revision A, released January 2019“Original Instructions”
For Research Use Only. Not for use in diagnostic procedures.

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Editor, Technical Publications
Thermo Fisher Scientific S.p.A.
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20090 Rodano (MI)
Italy
Editor, Technical Publications
Thermo Fisher Scientific CMD GC-GC/MS
2215 Grand Avenue Parkway
Austin TX 78728-3812
Unites States of America

Declaration
Manufacturer: Thermo Fisher Scientific
Thermo Fisher Scientific is the manufacturer of the instrument described in this manual and, as such, is responsible
for the instrument safety, reliability and performance only if:
• installation
• re-calibration
•changesandrepairs
have been carried out by authorized personnel and if:
• the local installation complies with local law regulations
• the instrument is used according to the instructions provided and if its operation is only entrusted to qualified
trained personnel
Thermo Fisher Scientific is not liable for any damages derived from the non-compliance with the aforementioned
recommendations.
Thermo Fisher Scientific S.p.A.
Strada Rivoltana, 20090 Rodano - Milan - Italy — Tel: [phone] - Fax: [phone]
Regulatory Compliance
Thermo Fisher Scientific performs complete testing and evaluation of its products to ensure full compliance with
applicable domestic and international regulations.
Thermo Fisher Scientific declares, under sole responsibility, that the product as originally delivered complies with
the requirements of the following applicable European Directives and carries the CE marking accordingly:
• Machinery Directive: 2006/42/EC
• EMC Directive: 2014/30/EU
• RoHS Directive: 2011/65/EU
… and conforms with the following product standards:
Compliant with applicable directives:
• Machinery Directive: 2006/42/EC
• EMC Directive: 2014/30/EU
• RoHS Directive: 2011/65/EU
Compliant with product standards:
EMC
– EN 61326-1:2013, IEC 61326-1:2012
– FCC rules: CFR no. 47 Part 15 Subpart B Section 15.107 and 15.109
Safety
– EN 61010-1:2010, IEC 61010-1:2010
– EN 61010-2-010: 2014, IEC 61010-2-010: 2014 (TriPlus 500 HS only)
– EN 61010-2-081: 2015, IEC 61010-2-081: 2015
– UL 61010-1:2012/R:2016-04

– UL 61010-2-010:2015 (TriPlus 500 HS only)
– UL 61010-2-081:2015
– CAN/CSA C22.2 No. 61010-1:2012/U2:2016-04
– CAN/CSA C22.2 No. 61010-2-010:2015 (TriPlus 500 HS only)
– CAN/CSA C22.2 No. 61010-2-081:2015
FCC Compliance Statement
Notice on Lifting and Handling of
Thermo Scientific Instruments
For your safety, and in compliance with international regulations, the physical handling of this Thermo Fisher
Scientific instrument requires a team effort to lift and/or move the instrument. This instrument is too heavy and/
or bulky for one person alone to handle safely.
Notice on the Proper Use of
Thermo Scientific Instruments
In compliance with international regulations: Use of this instrument in a manner not specified by Thermo Fisher
Scientific could impair any protection provided by the instrument.
Note This equipment has been tested and found to comply with the limits for a Class A digital device, pursuant
to Part 15 of the FCC Rules. These limits are designed to provide reasonable protection against harmful
interference when the equipment is operated in a commercial environment. This equipment generates, uses,
and can radiate radio frequency energy and, if not installed and used in accordance with instruction manual,
may cause harmful interference to radio communications. Operation of this equipment in a residential area is
likely to cause harmful interference in which case the user will be required to correct the interference at his own
expense.
THIS DEVICE COMPLIES WITH PART 15 OF THE FCC RULES. OPERATION IS SUBJECT TO THE
FOLLOWING TWO CONDITIONS: (1) THIS DEVICE MAY NOT CAUSE HARMFUL
INTERFERENCE, AND (2) THIS DEVICE MUST ACCEPT ANY INTERFERENCE RECEIVED,
INCLUDING INTERFERENCE THAT MAY CAUSE UNDESIRED OPERATION.
CAUTION Read and understand the various precautionary notes, signs, and symbols contained
inside this manual pertaining to the safe use and operation of this product before using the device.

Notice on the Susceptibility
to Electromagnetic Transmissions
Do not use radio frequency transmitters, such as mobile phones, in close proximity to the instrument.

WEEE Directive
2012/19/EU
Thermo Fisher Scientific has contracted with one or more recycling or disposal companies in each European Union
(EU) Member State, and these companies should dispose of or recycle this product. See www.thermofisher.com/
rohsweee for further information on Thermo Fisher Scientific compliance with these Directives and the recyclers in
your country.
Directive DEEE
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www.thermoscientific.com/rohsweee.

Thermo Scientific TriPlus 500 Headspace Sampler User Guide xi
C
Preface . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .xv
About Your System. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .xvi
Power Rating . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .xvi
Contacting Us . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . xvii
Related Documentation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . xvii
Safety Alerts and Important Information . . . . . . . . . . . . . . . . . . . . . . . . . . . . xvii
Special Notices . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . xvii
Safety Symbols and Signal Words . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .xviii
Instrument Markings and Symbols . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .xix
Safety Information and Warnings. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . xx
General Considerations . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . xx
Electrical Hazards . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .xxi
Other Hazards. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . xxii
Working with Toxic or other Harmful Compounds . . . . . . . . . . . . . . . . . .xxiii
Biological Hazards. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .xxiii
Maintenance . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . xxiv
Disposal. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . xxv
Writing Convention Used in This Guide . . . . . . . . . . . . . . . . . . . . . . . . . . . . xxv
Chapter 1 Getting Familiar with Your TriPlus 500 Headspace Sampler . . . . . . . . . . . . . . . . .1
Introduction . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 2
Instrument Basics . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 3
Label Location on the Instrument . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 7
Incubation Group . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8
Sample Vial Carousel. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8
Incubation Oven . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9
Sampling Path . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9
Pneumatic Connections. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11
Electrical Connections. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 12
Status Panel. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 13
TRACE 1310 GC User Interface . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 15
Vial Loader . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 16
Vial Loader Electronic Module . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 17
Contents

Contents
xii TriPlus 500 Headspace Sampler User Guide Thermo Scientific
Sample Trays. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 19
Vials Sequence Syntax . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 21
Positioning the Vials in the Sample Tray. . . . . . . . . . . . . . . . . . . . . . . . . . . . 22
Barcode Reader . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 23
About a Barcode Reader . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 23
Barcode Reader Description . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 24
Barcode Label Positioning . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 24
Heated/Cooled Tray . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 28
TriPlus 500 Web Interface . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 29
Chapter 2 Operating Principles. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .31
Introduction to the Headspace Technique. . . . . . . . . . . . . . . . . . . . . . . . . . . . . 32
Pneumatics . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 33
Analytical Cycle. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 34
Stand-by . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 34
Incubation. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 36
Pressurization . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 37
Leak Check . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 38
Loop Filling. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 39
Injection . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 40
Purging . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 42
Chapter 3 Setting Up Through the TRACE 1310 User Interface . . . . . . . . . . . . . . . . . . . . . . . .43
User Interface Overview . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 44
Menu Icons . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 44
Data Entry Keys . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 45
Shortcut Key . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 46
Cursor Keys. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 46
Configuring the Instrument . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 47
Editing the Instrument Method . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 49
Performing Samples Sequence . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 53
Monitoring the Instrument Status. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 55
Information Page. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 56
Chapter 4 Setting Up Through Chromeleon CDS . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .57
Configuring the TriPlus 500 HS Through Chromeleon CDS . . . . . . . . . . . . . . 58
Hardware Configuration . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 59
HS Configuration . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 59
Error Handling . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 60
Editing Method Parameters Through Chromeleon CDS. . . . . . . . . . . . . . . . . . 61
Incubation. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 62
Pressurization . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 62
Loop Filling. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 62
Injection . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 63
Venting and Purging . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 63

Contents
Thermo Scientific TriPlus 500 Headspace Sampler User Guide xiii
Chromeleon HS Sampler Control Panel . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 64
Vials Sequence. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 68
Chapter 5 Setting Up Through TraceFinder CDS . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .71
Configuring TriPlus 500 HS Through TraceFinder CDS . . . . . . . . . . . . . . . . . 72
Connection . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 73
Hardware Configuration . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 73
User Configuration . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 74
HS Configuration . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 74
Error Handling . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 74
Editing Method Parameters Through TraceFinder CDS . . . . . . . . . . . . . . . . . . 75
Incubation. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 76
Pressurization . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 76
Loop Filling. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 76
Injection . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 77
Venting and Purging . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 77
Advanced. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 78
TraceFinder Road Map Home Page Status Tabs . . . . . . . . . . . . . . . . . . . . . . . . 79
Chapter 6 Method Development . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .81
Usage Notes . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 81
Temperatures . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 82
Carrier Gas Optimization . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 82
Auxiliary Gas Pressure Optimization . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 82
Shaking Conditioning . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 83
Sample Vials . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 83
Sample Vial Septa . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 83
Vial Filling . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 84
Vial Closure. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 84
Missing and Wrong Size Vials . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 85
Matrix Effects . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 85
Principles of Multiple Headspace Extraction (MHE). . . . . . . . . . . . . . . . . . . 86
Processing a Sample Vial . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 91
User Actions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 92
Instrument Actions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 92
Method Developing Workflow . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 97
Analytical Troubleshooting General Guidelines. . . . . . . . . . . . . . . . . . . . . . . . 102
Chapter 7 Using the TriPlus 500 Web Interface. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .105
Introduction . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 106

Contents
xiv TriPlus 500 Headspace Sampler User Guide Thermo Scientific
Menu Bar . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 107
Login Menu . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 108
Administration Menu . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 108
Status Menu . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 109
Installation/Tools Menu . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 112
Service Menu. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 116
Manufacturing Menu . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 116
Logging Out of the TriPlus 500 Web Interface . . . . . . . . . . . . . . . . . . . . . . . . 116
Chapter 8 Ordering Parts . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .117

Thermo Scientific TriPlus 500 Headspace Sampler User Guide xv
P
Preface
This guide contains detailed information for the use of the Thermo Scientific™ TriPlus™ 500
Headspace Sampler (TriPlus 500 HS).
This guide is intended for frequent or new TriPlus 500 HS users who are experienced at using
automated systems to run existing analytical methods.
This guide is organized as follows:
•Chapter 1, “Getting Familiar with Your TriPlus 500 Headspace Sampler,” provides
information to familiarize you with your TriPlus 500 HS.
•Chapter 2, “Operating Principles,” describes the operating principles of the TriPlus 500
HS.
•Chapter 3, “Setting Up Through the TRACE 1310 User Interface,” contains the
instructions to configure your TriPlus 500 HS and to edit the parameters through the
TRACE 1310 GC user interface (touch screen) or through the TRACE 1310 Virtual
Touch Screen software.
•Chapter 4, “Setting Up Through Chromeleon CDS,” contains the instructions to
configure your TriPlus 500 HS and to edit the parameters through the Thermo
Scientific™ Chromeleon™ Chromatography Data System (CDS).
•Chapter 5, “Setting Up Through TraceFinder CDS,” contains the instructions to
configure your TriPlus 500 HS and to edit the parameters through the Thermo
Scientific™ TraceFinder™ Chromatography Data System (CDS).
•Chapter 6, “Method Development,” provides information for developing a method with
your TriPlus 500 HS.
•Chapter 7, “Using the TriPlus 500 Web Interface,” provides the instructions for using the
TriPlus 500 Web Interface.
•Chapter 8, “Ordering Parts,” contains part numbers for all the consumables and parts
available for your TriPlus 500 HS.
Note TriPlus 500 HS must be installed and set up properly before the Operating
Instructions can be used.

Preface
About Your System
xvi TriPlus 500 Headspace Sampler User Guide Thermo Scientific
•“Glossary,” contains definitions of terms used in this guide. It also includes abbreviations,
acronyms, metric prefixes, and symbols.
About Your System
Thermo Fisher Scientific systems operate safely and reliably under carefully controlled
environmental conditions. If the equipment is used in a manner not specified by the
manufacturer, the protections provided by the equipment might be impaired. If you maintain
a system outside the specifications listed in this guide, failures of many types, including
personal injury or death, might occur.
The repair of instrument failures caused by operation in a manner not specified by the
manufacturer is specifically excluded from the Standard Warranty and service contract
coverage.
Power Rating
TriPlus 500 HS alone:
• 100-240 Vac; 600 W; 50/60 Hz
Vial Loader
• 24 Vdc through a portable external power supply, level VI efficiency
Input 100-240 Vac; 50/60 Hz; 1.3 A — Output 24 Vdc; Power 90 W; 3.75 A
Detailed instrument specifications are in the Product Specifications Sheet.
Contents
•About Your System
•Power Rating
•Contacting Us
•Related Documentation
•Safety Information and Warnings
•Instrument Markings and Symbols
•Safety Information and Warnings
•Writing Convention Used in This Guide
WARNING You must only use the portable external power supply provided with the
instrument by Thermo Fisher Scientific.

Preface
Contacting Us
Thermo Scientific TriPlus 500 Headspace Sampler User Guide xvii
Contacting Us
There are several ways to contact Thermo Fisher Scientific for the information you need.
To find out more about our products
Go to http://www.thermofisher.com for information about our products.
To get local contact information for sales or service
Go to http://www.unitylabservice.com/en/home.html
Related Documentation
In addition to this guide, Thermo Scientific™ provides the following documents for TriPlus
500 HS.
•TriPlus 500 Headspace Sampler Preinstallation Requirements Guide, PN 31716105
•TriPlus 500 Headspace Sampler Hardware Manual, PN 31716107
•TriPlus 500 Headspace Sampler Spare Parts Catalog, PN 31716108
To suggest ways we can improve the documentation, follow this link to complete our Reader’s
Survey.
Safety Alerts and Important Information
Make sure you follow the precautionary notices presented in this manual. The safety and
other special notices appear in boxes.
Special Notices
Notices includes the following:
IMPORTANT Highlights information necessary to prevent damage to software, loss of
data, or invalid test results; or might contain information that is critical for optimal
performance of the system.
Note Emphasizes important information about a task.
Tip Helpful information that can make a task easier.

Preface
Safety Alerts and Important Information
xviii TriPlus 500 Headspace Sampler User Guide Thermo Scientific
Safety Symbols and Signal Words
All safety symbols are followed by WARNING or CAUTION, which indicates the degree of risk
for personal injury, instrument damage, or both. Cautions and warnings are following by a
descriptor, such as BURN HAZARD. A WARNING is intended to prevent improper actions that
could cause personal injury. Whereas, a CAUTION is intended to prevent improper actions
that might cause personal injury, instrument damage, or both. You can find the following
safety symbols on your instrument, or in this guide:
Symbol Descriptor
BIOHAZARD: Indicates that a biohazard will, could, or might occur.
BURN HAZARD: Alerts you to the presence of a hot surface that could or
might cause burn injuries.
ELECTRICAL SHOCK HAZARD: Indicates that an electrical shock could or
might occur.
FIRE HAZARD: Indicates a risk of fire or flammability could or might occur.
EXPLOSION HAZARD. Indicates an explosion hazard. This symbol indicates
this risk could or might cause physical injury.
FLAMMABLE GAS HAZARD. Alerts you to gases that are compressed,
liquefied or dissolved under pressure and can ignite on contact with an
ignition source. This symbol indicates this risk could or might cause physical
injury.
GLOVES REQUIRED: Indicates that you must wear gloves when performing a
task or physical injury could or might occur.
CLOTHING REQUIRED. Indicates that you should wear a work clothing when
performing a task or else physical injury could or might occur.
BOOTS REQUIRED. Indicates that you must wear boots when performing a
task or else physical injury could or might occur.
MATERIAL AND EYE HAZARD. Indicates you must wear eye protection
when performing a task.
HAND AND CHEMICAL HAZARD: Indicates that chemical damage or
physical injury could or might occur.
HARMFUL. Indicates that the presence of harmful material will, could, or
might occur.
INSTRUMENT DAMAGE: Indicates that damage to the instrument or
component might occur. This damage might not be covered under the
standard warranty.

Preface
Instrument Markings and Symbols
Thermo Scientific TriPlus 500 Headspace Sampler User Guide xix
Instrument Markings and Symbols
Table 1 explains the symbols used on Thermo Fisher Scientific instruments. Only a few of
them are used on TriPlus 500 HS, which are annotated with an asterisk below.
LIFTING HAZARD. Indicates that a physical injury could or might occur if
two or more people do not lift an object.
MATERIAL AND EYE HAZARD: Indicates that eye damage could or might
occur.
READ MANUAL: Alerts you to carefully read your instrument’s
documentation to ensure your safety and the instrument’s operational ability.
Failing to carefully read the documentation could or might put you at risk for
a physical injury.
TOXIC SUBSTANCES HAZARD: Indicates that exposure to a toxic substance
could occur and that exposure could or might cause personal injury or death.
RADIOACTIVE HAZARD. Indicates that the presence of radioactive material
could or might occur.
For the prevention of personal injury, this general warning symbol precedes
the WARNING safety alert word and meets the ISO 3864-2 standard. In the
vocabulary of ANSI Z535 signs, this symbol indicates a possible personal
injury hazard exists if the instrument is improperly used or if unsafe actions
occur. This symbol and another appropriate safety symbol alerts you to an
imminent or potential hazard that could cause personal injury.
Table 1. Instrument Marking and Symbols (Sheet 1 of 2)
Symbol Description
Direct Current
*Alternating Current
Both direct and alternating current
Three-phase alternating current
Earth (ground) terminal
Protective conductor terminal
Frame or chassis terminal
Equipotentiality
3

Preface
Safety Information and Warnings
xx TriPlus 500 Headspace Sampler User Guide Thermo Scientific
Safety Information and Warnings
This safety guide raises awareness of potential safety issues and general points for
consideration for Thermo Fisher Scientific representatives during installation, and repair of
TriPlus 500 HS, or parts of it (following the life cycle principle), as well as for the end user of
TriPlus 500 HS in the lab during the learning phase, and in routine work.
General Considerations
• Before a unit is put to use, consult the TriPlus 500 HS manuals and related documents
under all circumstances.
• Changes or modifications to this unit not expressly approved by the party responsible for
compliance, could void your authority to operate the equipment.
*On (Supply)
*Off (Supply)
Equipment protected throughout by DOUBLE INSULATION or
REINFORCED INSULATION (Equivalent to Class II of IEC 536)
Fuse
*Instruction manual symbol affixed to product. Indicates that the you must
refer to the manual for specific WARNING or CAUTION information to
avoid personal injury or damage to the product.
Caution, risk of electric shock
*Caution, hot surface
*Caution, biohazard
*Symbol in compliance to the Directive 2012/19/EU on Waste Electrical
and Electronic Equipment (WEEE) placed on the European market after
August, 13, 2005.
Table 1. Instrument Marking and Symbols (Sheet 2 of 2)
Symbol Description
IMPORTANT Read this section first before operating with TriPlus 500 HS.