Thermo Scientific HyPerforma S.U.B. TK User manual

Thermo Scientific
HyPerforma S.U.B. TK (Resistive)
Quick Start Guide

Thermo Scientific
HyPerforma S.U.B. TK (Resistive)
Quick Start Guide
Rev. 1.0

Thermo Scientific 1S.U.B. TK Resistive Quick Start Guide
Quick Start Guide
1.1 Orientation and
Location
Section 1 Hardware Installation
e Single-Use Bioreactor hardware is shipped directly from the
manufacturer and will arrive crated. For unpacking instructions and
detailed contents of the crate, please refer to the uncrating instructions
that came with the shipment. Be sure to follow the unpacking instructions
provided and retain all packaging materials.
Tools Needed:
• Allen wrench provided in shipping documentation packet
• Adjustable wrench needed for leveling feet (not supplied)
a. Position reactor and tower in desired location and lock wheels
ensuring availability of needed utilities (power, gas, etc.). Right or
left hand orientation was determined at time of order. Ensure that
tower is located on the same side as the cable and tubing organizer.
b. Before running cables, note that the cable and tubing organizer
has an adjustment feature to keep the cables lined up with the
rear of the control tower. e arm has a slide that can extend and
retract manually using the clamping knob.
WARNING: Any procedures that require the control box to be open
should be performed with the main electrical disconnect in the locked out
position and all power sources removed from the control box. For operator
safety, secure the location of the S.U.B. outer support container
by disabling the swivel casters before servicing.
Figure 1. Cable and Tubing Organizer Figure 2. Cable and Tubing
Organizer Adjustment Feature

Thermo Scientific 2S.U.B. TK Resistive Quick Start Guide
Quick Start Guide
1.2 Attaching Cables
and Gas Lines
Figure 3. Back panel labeling
• Connect Motor Power cable into designated Tower connector (● ● ●)
• Connect Motor Feedback cable into designated Tower connector (● ● ●)
• Connect Load Cell cable into designated Tower connector (● ● ●)
• Use the three (3) color-coded, labeled gas lines and connect each
line to the respective colored dot source on the Tower.
i. Overlay Sparge – Black (●)
ii. Micro Sparge – Red (●)
iii. Macro Sparge - White (●)
• Service gas pressure should be a minimum of 30 psi, and
a maximum of 35 psi for proper operation. Verify that gas
connections are secure and there are no leaks (e.g. Snoop or similar
product to detect leaks).
• Connect the RTD (temperature probe) cable ( ● ● ● ). is cable
should be in a box that was shipped with the tower.
Connect the corresponding facility gas sources to the designated
connectors on the Tower. e connectors are fractional (inches), so if the
facility uses metric lines, simply use the adapters provided in the plastic
bag inside the Tower (6 mm. to 1/4” adapters).

Thermo Scientific 3S.U.B. TK Resistive Quick Start Guide
Quick Start Guide
• Attach probe clips to probe window (front of the S.U.B.). e
probe clips were shipped with the S.U.B. unit.
Figure 4. 250 L S.U.B. TK System
Position and attach various hardware items:
• Secure the tool bracket to the tank. is position is not critical,
but would be convenient near the motor. Place the ratchet and the
custom open end wrench in the tool holder. Attach drive shaft, as
shown.
• Attach the adjustable vent lter heater bracket using the clamp
lever handles. Position this hardware directly opposite of the
motor, as shown.

Thermo Scientific 4S.U.B. TK Resistive Quick Start Guide
Quick Start Guide
NOTE: Before connecting cables, route them through the tubing and
cable organizer rst
• If you intend to use the Finesse TruFluor single-use sensors, route
the appropriate cables from the Tower into the cable management
arm and down to the sensor window area on the S.U.B. ese
cables will already be attached to the rear of the Control Tower
via a liquid tight connection and have the following designation,
DO-2 and pH-2 (No color code).
• If you intend to use traditional re-usable sensing probes, route the
appropriate cables in the same manner as described above for the
single-use sensors. Ensure they are plugged into the back of the
control tower (● DO-1 ●, ● pH-1 ●).
• Locate the bag pressure sensor cable on the Tower (● ● ●) and
route it via the cable management arm and up toward the top arm
of the tank.
If using a vent heater make the following connections.
• Connect cable to labeled VH-1 POWER (● ●) power source on
Towe r.
• Connect Yellow ermocouple to labeled connection on Tower
VH-1 FDBK (● ●)
• Route cable through cable management arm and up to vent heater
brackets on top of S.U.B. unit.
• If using a second vent heater, use VH-2 connections
VH-2 POWER (● ● ●) and VH-2 FDBK (● ● ●) and route
parallel with VH-1 cable.
Vent Filter Heater
If using a resistive heater make the following connections.
• Connect cable labeled RES HEATER (● ● ●) to the power
source on the Tower
• Connect cable labeled HEATER POWER IN (● ● ●) into
designated tower connector.
Resistive Heater
Power Connection

Thermo Scientific 5S.U.B. TK Resistive Quick Start Guide
Quick Start Guide
Before connecting power, verify, again, all gas lines were checked and not
leaking.
• Connect main power cord (no color code) to correct utility source.
• It is strongly recommended that a UPS (Uninterrupted Power
Supply) (● ● ●) be used. e purpose of this connection is to
maintain power to the controls during a power outage.
• If UPS power is not available or not used, then the UPS Jumper
connector must be put in place of the UPS cable.
1.3 Power
Connections
UPS Jumper
Figure 5. UPS Jumper
Ensure the S.U.B. unit is in its intended position.
• Lock the vessel in place by lowering the leveling feet. Ensure that
unit is level before operation.
• Using the Allen wrench provided with S.U.B. unit shipping
documents, remove the load cell shipping plates and secure them
in the bracket at the rear of the control tower. ere are two ¼-20
socket head cap screws that need to be removed from each load
cell protection plate. Move the tower to desired position and lock
in place and level using the leveling feet.
1.4 Securing Position
of Control Tower and
S.U.B.

Thermo Scientific 6S.U.B. TK Resistive Quick Start Guide
Quick Start Guide
• Refer to User’s Guide for detailed instructions on calibration steps
(load cells, sensors, etc).
• A virtual keyboard icon is located at the bottom of the window
display for notes, values, etc.
• Start by turning on the MAIN DISCONNECT switch located at
the back of the control tower.
• Ensure the Emergency Stop (located at the front of the tower) is
not activated (should be in the out position). If it is engaged, then
twist to disengage. Note that the E-stop light will remain red, even
though it is disengaged, until the next step has been completed.
• Turn power on by pressing the green Start/Re-set button located at
the front of the tower behind the operator screen. E-stop light will
then turn o.
• Note that a green operation light, located at the back of the tower,
will also turn on.
e monitor can be tilted up and down and rotate left to right.
1.5 HMI (Monitor)
1.6 Power Up the
System
1.7 System Will
Begin Booting Up

Thermo Scientific 7S.U.B. TK Resistive Quick Start Guide
Quick Start Guide
Section 2 BPC Installation
1. Carefully unpack the S.U.B. BioProcess Container (BPC) from its
two protective poly bags.
2. Uncoil and remove protective packaging from the sparger, drain,
and auxiliary lines on the bottom of the BPC. Don’t remove the
protective packaging on top of the BPC at this time.
3. Carefully lower the bottom end of the BPC into the S.U.B. with
any tubing sets on the top side laying over the side until the
bearing assembly is level with the drive shaft motor.
4. Remove the protective black cap from the bearing assembly and
slide into the retention mechanism on the agitation motor and
lock it into place.
Figure 6. Bearing port inserted into motor mount slot and clamp

Thermo Scientific 8S.U.B. TK Resistive Quick Start Guide
Quick Start Guide
5. Guide bottom tubing lineset through their proper channels:
• Insert frit and open pipe sparger lines through the sparge plate
(round, white plastic with slots) rst, before locating it in the
base of unit.
• Insert drain line through bottom front face directly below
agitation motor. Hang the drain line coil on the arm provided.
Also route the sample line through the snap in slots on the
drain coil arm.
• Attach BPC positioning tabs (bottom of chamber) onto
the alignment pins located at the base of the S.U.B. tank.
Note that the 50 L BPC does not require this step and is not
recommended, but has tab holders available, if desired.
• Insert sensors, KleenPaks, and auxiliary linesets through the
“probe belt opening on the tank”.
6. Uncover vent lters, wrap heating blankets (if used) around
the lters, attach/adjust vent bracket, and mount vent lters in
bracket.
Figure 7. BPC tab holders used for proper BPC positioning
Figure 8. Filter being placed in bracket

Thermo Scientific 9S.U.B. TK Resistive Quick Start Guide
Quick Start Guide
7. Route the overlay sparge set (located at the top of the BPC marked
“Overlay Sparge”) through the cable and tubing organizer arm to
the top of the main beam of the cable and tubing organizer and
connect to the tting marked OVERLAY SPARGE. is will be
the lineset that will be used to pre-ll the BPC with air, so ensure
that the clamp on this lineset is released.
8. Route the Micro Sparge Line (labeled), located at the bottom
of the BPC, up through the main beam of the cable and tubing
organizer using the black snap in features. Connect to the correct
gas tting labeled MICRO SPARGE. Unclamp this lineset when
ready to sparge through frit.
9. Route Macro Sparge line (labeled), located at the bottom of
the BPC, running parallel to the Micro Sparge Line, using the
same snap in features. Connect to the correct gas tting labeled
MACRO SPARGE. Ensure the clamp is released on this line, as
well before operation.
NOTE: To reduce the risk of lter membrane wetting, position
each sparge lter above the low point of the sparge line.
10. Connect the pressure sensor cable onto the sensor located at the
top of the BPC.
Figure 10. Pressure sensor
Figure 9. BPC linesets

Thermo Scientific 10S.U.B. TK Resistive Quick Start Guide
Quick Start Guide
11. Ensure the cable has been routed through the cable and tubing
organizer and connect to the tting located at the rear of the
control cabinet labeled BAG PRESS (● ● ●).
12. IMPORTANT Disengage any clamps on the lineset for the
exhaust lter(s). If the clamps are left engaged, the BPC could
burst when lling it with air or liquid.
13. Position and close a bar clamp (Part No. SV20664.01) on the
bottom drain line as close as possible to the BPC port (Figure 11).
Also place bar clamps on probe ports intended for conventional
probes (Figure 12).
14. Insert RTD or selected temperature sensor into thermo-well
(Figure 13, Figure 14).
• Place small amount of glycerol (0.5 mL) in well to aid in heat
transfer.
• Sensor should be inserted until base of probe meets the mouth
of the thermo-well.
• Secure by twisting the luer lock collar. e thermo-well will
stretch slightly when RTD is seated.
Figure 13
Figure 11
Figure 14
Figure 3.12

Thermo Scientific 11S.U.B. TK Resistive Quick Start Guide
Quick Start Guide
With the software booted up, click on the “HOME” button on the upper
left side of the screen. In the top right hand corner of the screen, click on
the icon “Guest.”
en click on the User pull down menu and select “Administrator.”
Enter the correct Password, which is “admin.”
To type information without plugging a USB keyboard into the control
tower, click on the miniature keyboard icon located at the bottom middle
of the screen. is will bring up a touch screen keyboard. When nished
using the touch screen keyboard just click on the upper right hand corner
to close it. Note, you must bring up the keyboard FIRST before opening
a drop down menu. Also note that the keyboard can be moved and
repositioned.
Figure 15 Initial TruBio View With Log On Breakout
Section 3 Software Start Up

Thermo Scientific 12S.U.B. TK Resistive Quick Start Guide
Quick Start Guide
Now the heading should read “Administrator.”
e next step is to load the correct conguration le for the SUB unit.
Click on the “Congure” button on the left side of the screen.
NOTE: you can also click on the yellow “Load Conguration File” button
when rst turning on the system.
Click the tab on the top that reads “File.”
Select the appropriate S.U.B. TK system to be used by clicking to highlight
it.
• ermo Turnkey 250L Electric SUB
• ermo Turnkey 250L Jacketed SUB
• ermo Turnkey 50L Electric SUB
• ermo Turnkey 50L Jacketed SUB
Load the new conguration by clicking the “Load” button on the right.
Select “Yes” when the pop up window appears.
e screen automatically jumps to the “Home” display window.
Figure 16 Configure Files View

Thermo Scientific 13S.U.B. TK Resistive Quick Start Guide
Quick Start Guide
Figure 17 “Home” Monitor View

Thermo Scientific 14S.U.B. TK Resistive Quick Start Guide
Quick Start Guide
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