Thermo Scientific 1500 Series User manual

Operating Instructions
3848331 • Revision B • June 2019
1500 Series B2
Class II, Type B2 
Biological Safety Cabinet

IMPORTANT Read this instruction manual. Failure to follow the instructions in this manual can result in 
damage to the unit, injury to operating personnel and poor equipment performance.
CAUTION All internal adjustments and maintenance must be performed by qualified service personnel.
Material in this manual is for informational purposes only. The contents and the product it describes are 
subject to change without notice. Thermo Fisher Scientific makes no representations or warranties with
respect to this manual. In no event shall Thermo Fisher Scientific be held liable for any damages, direct or 
incidental, arising from or related to the use of this manual.
© 2019 Thermo Fisher Scientific Inc. All rights reserved.


Contents
Preface ........................................................................ 1
Legal Information...................................................... 1
Warranty .................................................................. 1
Introduction ................................................................. 2
Intended Use............................................................ 2
Instruction of the Operating Personnel...................... 2
Safety Precautions....................................................... 4
Prerequisites................................................................ 6
Space Requirements................................................ 6
Clearance Requirements .......................................... 6
Location Requirements ............................................ 6
Exhaust System Requirements................................. 6
Electrical Requirements............................................ 7
Service Line Requirements....................................... 7
Getting Started ............................................................ 8
Unpacking the Biosafety Cabinet.............................. 8
Preparing the Biosafety Cabinet for Operation.......... 8
Moving and Lifting the Cabinet ................................. 9
Installing the Biosafety Cabinet on 
an Existing Work Surface ......................................... 9
Installing the Cabinet on a Thermo Fisher Scientific 
Base Stand .............................................................. 9
Telescoping Base Stands......................................... 9
Exhaust System Connections................................... 10
Exhaust System Requirements................................. 11



Connecting the Biosafety Cabinet to 
Utility Service Lines .................................................. 11
Using the SmartPortTM Cord & Cable Portal ............. 11
Installing the Drain Valve........................................... 12
Initial Certification ..................................................... 12
Performance Features.................................................. 14
HEPA Filters............................................................. 14
ULPA Filters ............................................................. 14
Laminar Airflow ........................................................ 14
Directional Airflow..................................................... 15
Motor/Blower ........................................................... 15
Airflow Sensor.......................................................... 15
Cabinet Grilles, Ductwork and 
Air Balance Controls................................................. 16
Ultraviolet (UV) Lamp................................................ 16
Using the Cabinet ........................................................ 17
System Reset Switch ............................................... 17
Information Center ................................................... 17
Alarm Screens.......................................................... 18
Operating the Sliding Sash ....................................... 19
Starting the Biosafety Cabinet .................................. 19
Using the Cabinet Keypad........................................ 20
Navigating the Biosafety Cabinet Menu Screens....... 20
Working In the Biosafety Cabinet.............................. 25
Maintaining the Cabinet ............................................... 27
Service Operations................................................... 27
Troubleshooting........................................................... 31
Appendix A: Components............................................ 33

Appendix B: Dimensions.............................................. 35
Appendix C: Specifications .......................................... 36
Environmental Conditions......................................... 36
Appendix D: Accessories ............................................. 37
Telescoping Base Stands......................................... 37
Appendix E: Quick Chart.............................................. 39

1500 Series Class II, Type B2 Biological Safety Cabinet Preface | 1
Preface
Read through the information given in these operating
instructions manual before you begin installation and use of
your “Biological Safety Cabinet”. Thermo Fisher Scientific
does not assume any liability for damage due to incorrect
operation or use of the system other than the intended use. In
case of conflicting translations into foreign languages the
English-language version of these operating instructions shall
be binding.
Thermo Fisher Scientific Inc. provides this document to its
customers with a product purchase to use in the product
operation. This document is copyright protected. Any
reproduction of the whole or any part of this document is
strictly prohibited, except with the written authorization of
Thermo Fisher Scientific Inc. The contents of this document
are subject to change without notice. All technical information
in this document is for reference purposes only. System
configurations and specifications in this document supersede
all previous information received by the purchaser.
Thermo Fisher Scientific Inc. makes no representations that
this document is complete, accurate or error-free and
assumes no responsibility and will not be liable for any errors,
omissions, damage or loss that might result from any use of
this document, even if the information in the document is
followed properly.
This document is not part of any sales contract between
Thermo Fisher Scientific Inc. and a purchaser. This document
shall not govern or modify any Terms and Conditions of Sale,
which Terms and Conditions of Sale shall govern all
conflicting information between the two documents.
Legal Information
Note: Specifications, terms and pricing are subject to
change. Not all products are available in all countries. Consult
your local sales representative for details.
Warranty
Thermo Fisher Scientific warrants the operational safety and
functions of the biosafety cabinet only under the condition
that:
• The device is operated and serviced exclusively in
accordance with its intended purpose and as described
in these operating instructions
• The device is not modified
• Only original spare parts and accessories that have been
approved by Thermo Fisher Scientific are used
• Inspections and maintenance are performed at the
specified intervals
• An installation certification is performed prior to the initial
operation of the device and that a repeat certification is
performed on the occasion of all inspections and repairs.
The warranty is valid from the date of delivery of the device to
the operator.

2 | Introduction 1500 Series Class II, Type B2 Biological Safety Cabinet
Introduction
Thermo Fisher Scientific™ 1500 Series B2 Class II, Type B2
Biosafety Cabinet™ has been tested and certified in
accordance to NSF/ANSI 49, and is designed to protect the
user, the environment, and your research from harmful
substances and cross-contamination. This user’s manual
provides instructions on how to use the 1500 Series B2 most
effectively and safely.
The 1500 Series B2 offers a unique range of product features
that will enhance your safety and improve overall operating
efficiency. Should you have any questions on using this
product or need further explanation of any of its features,
contact Thermo Fisher Scientific technical support group at
the phone numbers listed on the following pages.
Postal address USA
Thermo Fisher Scientific Inc. (Asheville) LLC
401 Millcreek Road, Box 649 
Marietta, OH 45750
United States
Enquiries from North America, Latin America and Asia
Pacific
Phone: [phone]
Fax:[phone]
Email: [e-mail]
Intended Use
Correct Use:
The biosafety cabinet is a laboratory device for installation and
operation in microbiological and biotechnical laboratories of
biosafety levels 1, 2, and 3. It has been designed as a Class II
Biosafety Cabinet, Type B2, in accordance with NSF/ANSI
49.
Depending on the hazard level of the agents involved, the
operator must prepare in writing appropriate decontamination
procedures for the device and the accessories used in the
sample chamber.
Prior to the initial operation of the cabinet, the operator must
perform an installation test, or certification. According the
NSF, the test result must be documented by a test report.
The cabinet must only be used after it is in compliance with
the operating parameters as specified by Thermo Fisher
Scientific.
After any changes to the installation conditions and after any
modification to the technical system, the certification test
must be repeated and the test result must be documented in
a way that demonstrates all operating parameters are in
compliance with those specified by Thermo Fisher Scientific.
Incorrect Use:
To ensure optimum safety to the user and the environment,
the biosafety cabinet should not be used in laboratories that
do not comply with the requirements of biosafety levels 1, 2 or
3.
The device must not be operated as a Class II, Type B2
Biosafety Cabinet if:
• No repeat test is performed after changes to the
installation conditions or after modifications to the
technical system,
• The alarm system of the device has issued a failure
message and the cause for the failure has not been
repaired.
The alarm system must not be tampered with or disabled. 
If alarm system components have been removed or disabled
for service or repairs, the device must only be released for
operation after all alarm system components are confirmed to
be functioning properly.
The HEPA filters installed in the device are not capable of
separating gaseous substances. Therefore, avoid storing or
processing gases or gas-releasing substances in the device:
• Which in quantity or concentration are toxic,
• If a reduction with other substances may result in
hazardous toxic concentrations or formation of toxic
gases, that may form combustive or explosive mixtures in
combination with air.
Instruction of the
Operating Personnel
The operating instructions describe the biosafety cabinet
1500 Series B2, and applies to the models:
• 4-foot models 1510 / 1511 / 1515 / 1516 / 1517 / 1518
• 6-foot models 1560 / 1561 / 1562 / 1563 / 1564 / 1565
This biosafety cabinet has been manufactured in keeping with
the latest technological developments and has been tested
before delivery for its correct function. It may, however,
present potential hazards if it is not used according to the
intended purpose or outside of operating parameters.
Therefore, the following procedures must always be observed
to prevent accidents:

1500 Series Class II, Type B2 Biological Safety Cabinet Introduction | 3
• The biosafety cabinet should be operated by authorized
personnel who have been trained on the use and
maintenance of this product.
• For any operation of this device, it is recommended that
the operator prepares clear and concise written
instructions in the language of the operating and cleaning
personnel based on these operating instructions,
applicable safety data sheets, plant hygiene guidelines,
and technical regulations. Most importantly:
• which decontamination measures are to be applied
for the cabinet and accessories
• which protective measures apply while specific
agents are used
• which measures are to be taken in the case of an
accident.
• To ensure safety during the repair of the cabinet, servicing
of the equipment must be carried out by trained and
authorized personnel.

4 | Safety Precautions 1500 Series Class II, Type B2 Biological Safety Cabinet
Safety Precautions
In this manual, the following symbols and conventions are
used.
Below are important safety precautions that apply to this
product.
CAUTION: Do not position the unit so that it is
difficult to operate the main disconnect device.
CAUTION: Electrical outlets in the cabinet are
restricted to 5 amps (115 V models) or 2 amps
(230 V models) maximum current.
CAUTION: Do not use any detachable power
cord that is not adequately rated for the unit.
WARNING: Do not attempt to lift the Biosafety
Cabinet by the side panels, damage will occur.
Note: The Biosafety Cabinet should be certified
by a certification technician before its initial use.
The cabinet should be recertified whenever it is
relocated, serviced or at least annually thereafter.
Filter integrity and airflow performance should be
verified before using the cabinet.
WARNING: The Total Exhaust Biosafety Cabinet
is designed to be connected to an appropriate
exhaust system. Without verified inflow velocity,
the cabinet may NOT contain hazardous
particulate or gases. Do not attempt to operate it
when it is not connected to an appropriate
exhaust system that has been inspected by a
qualified certifier.
WARNING: Type B2 Biosafety Cabinets rely on
external blowers (usually on the roof of a building)
to exhaust 100% of the air entering the cabinet. If
the building exhaust blower fails or is mistakenly
turned OFF, the cabinet will not provide
containment of hazardous materials.
WARNING: Do not use the cabinet until the
problem has been corrected.
CAUTION: Only lift and support the cabinet at
the stainless steel sides and back. Damage will
occur if lifted or supported on the front edge.
CAUTION: Some internal components of the
Biosafety Cabinet may become contaminated
during operation of the unit. Only experienced
personnel competent in decontamination
procedures should decontaminate the cabinet
before servicing these components. If you have
any questions regarding certification agencies, or
need assistance in locating one, contact Thermo
Fisher Scientific.
CAUTION: DO NOT load more than 50 lbs. (23
Kg) in the work area. Exceeding this limit may
damage the work surface and its supports.
Excessive weight in the cabinet may increase the
risk of it overturning, or failure of hydraulic lift
stands, resulting in the cabinet and stand
overturning. If your application requires loading
more than 50 lbs., contact Thermo Fisher
Scientific.
CAUTION: Ensure that the cabinet is connected
to electrical service in accordance with local and
national electrical codes. Failure to do so may
create a fire or electrical hazard. Do not remove
or service any electrical components without first
disconnecting the Biosafety Cabinet from
electrical service.
CAUTION: Avoid the use of flammable gases or
solvents in the Biosafety Cabinet. Care must be
taken to ensure against the concentration of
flammable or explosive gases or vapors. An open
flame should NOT be used in the Biosafety
Cabinet. Open flames will disrupt airflow
patterns, burn the HEPA filter and/or damage the
filter’s adhesive. Gases under high pressure
should not be used in the Biosafety Cabinet, as
they may disrupt its airflow patterns.
CAUTION: HEPA filters only remove particulate
matter. Operations generating volatile toxic
chemicals or radionuclides must be evaluated
carefully.
CAUTION: The media of HEPA filters is fragile
and should not be touched. Avoid puncturing
either HEPA filter during installation or normal
operation. If you suspect that a HEPA filter has
been damaged, DO NOT use the cabinet;
contact a local certification agency or Thermo
Fisher Scientific.

1500 Series Class II, Type B2 Biological Safety Cabinet Safety Precautions | 5
CAUTION: The HEPA filters in the Biosafety
Cabinet will gradually accumulate airborne
particulate matter from the room and from work
performed in the cabinet. The rate of
accumulation will depend upon the cleanliness of
the room air, operating time and the nature of
work being done in the cabinet. The Filter Gauge
accurately displays the amount of filter life
remaining for the cabinet downflow filter.
CAUTION: Proper operation of the cabinet
depends largely upon its location and the
operator’s work habits. Consult the Installation
and Normal Operation sections of this manual for
further details.
CAUTION: Avoid direct exposure of plastic or
coated materials to ultraviolet (UV) radiation.
Never bypass the UV safety interlock that only
allows the UV light to work when the sash is
closed. When surface disinfecting the Biosafety
Cabinet:

Avoid splashing the disinfecting solution on skin
or clothing.

Ensure adequate ventilation.

Carefully follow the disinfectant’s safety
instructions.

Always dispose of disinfecting solutions in
accordance with local and national laws.
CAUTION: DO NOT allow disinfectants with
high concentrations of free chlorine to contact
the stainless steel components of the Biosafety
Cabinet for a long period of time. Free chlorine
will corrode stainless steel after extended
contact.
CAUTION: Biosafety Cabinets should be
decontaminated for any of the following reasons:

Before maintenance work requiring entry into
contaminated areas.

Before HEPA filter changes.

Before performing certification tests requiring
entry into contaminated areas.

Before relocating the cabinet.

Before changing research programs.

After the gross spill of biohazardous material or
toxic chemicals.