Thermo Scientific 1500 Series User manual

Operating Instructions
3848331 • Revision B • June 2019
1500 Series B2
Class II, Type B2 
Biological Safety Cabinet

IMPORTANT Read this instruction manual. Failure to follow the instructions in this manual can result in 
damage to the unit, injury to operating personnel and poor equipment performance.
CAUTION All internal adjustments and maintenance must be performed by qualified service personnel.
Material in this manual is for informational purposes only. The contents and the product it describes are 
subject to change without notice. Thermo Fisher Scientific makes no representations or warranties with
respect to this manual. In no event shall Thermo Fisher Scientific be held liable for any damages, direct or 
incidental, arising from or related to the use of this manual.
© 2019 Thermo Fisher Scientific Inc. All rights reserved.


Contents
Preface ........................................................................ 1
Legal Information...................................................... 1
Warranty .................................................................. 1
Introduction ................................................................. 2
Intended Use............................................................ 2
Instruction of the Operating Personnel...................... 2
Safety Precautions....................................................... 4
Prerequisites................................................................ 6
Space Requirements................................................ 6
Clearance Requirements .......................................... 6
Location Requirements ............................................ 6
Exhaust System Requirements................................. 6
Electrical Requirements............................................ 7
Service Line Requirements....................................... 7
Getting Started ............................................................ 8
Unpacking the Biosafety Cabinet.............................. 8
Preparing the Biosafety Cabinet for Operation.......... 8
Moving and Lifting the Cabinet ................................. 9
Installing the Biosafety Cabinet on 
an Existing Work Surface ......................................... 9
Installing the Cabinet on a Thermo Fisher Scientific 
Base Stand .............................................................. 9
Telescoping Base Stands......................................... 9
Exhaust System Connections................................... 10
Exhaust System Requirements................................. 11



Connecting the Biosafety Cabinet to 
Utility Service Lines .................................................. 11
Using the SmartPortTM Cord & Cable Portal ............. 11
Installing the Drain Valve........................................... 12
Initial Certification ..................................................... 12
Performance Features.................................................. 14
HEPA Filters............................................................. 14
ULPA Filters ............................................................. 14
Laminar Airflow ........................................................ 14
Directional Airflow..................................................... 15
Motor/Blower ........................................................... 15
Airflow Sensor.......................................................... 15
Cabinet Grilles, Ductwork and 
Air Balance Controls................................................. 16
Ultraviolet (UV) Lamp................................................ 16
Using the Cabinet ........................................................ 17
System Reset Switch ............................................... 17
Information Center ................................................... 17
Alarm Screens.......................................................... 18
Operating the Sliding Sash ....................................... 19
Starting the Biosafety Cabinet .................................. 19
Using the Cabinet Keypad........................................ 20
Navigating the Biosafety Cabinet Menu Screens....... 20
Working In the Biosafety Cabinet.............................. 25
Maintaining the Cabinet ............................................... 27
Service Operations................................................... 27
Troubleshooting........................................................... 31
Appendix A: Components............................................ 33

Appendix B: Dimensions.............................................. 35
Appendix C: Specifications .......................................... 36
Environmental Conditions......................................... 36
Appendix D: Accessories ............................................. 37
Telescoping Base Stands......................................... 37
Appendix E: Quick Chart.............................................. 39

1500 Series Class II, Type B2 Biological Safety Cabinet Preface | 1
Preface
Read through the information given in these operating
instructions manual before you begin installation and use of
your “Biological Safety Cabinet”. Thermo Fisher Scientific
does not assume any liability for damage due to incorrect
operation or use of the system other than the intended use. In
case of conflicting translations into foreign languages the
English-language version of these operating instructions shall
be binding.
Thermo Fisher Scientific Inc. provides this document to its
customers with a product purchase to use in the product
operation. This document is copyright protected. Any
reproduction of the whole or any part of this document is
strictly prohibited, except with the written authorization of
Thermo Fisher Scientific Inc. The contents of this document
are subject to change without notice. All technical information
in this document is for reference purposes only. System
configurations and specifications in this document supersede
all previous information received by the purchaser.
Thermo Fisher Scientific Inc. makes no representations that
this document is complete, accurate or error-free and
assumes no responsibility and will not be liable for any errors,
omissions, damage or loss that might result from any use of
this document, even if the information in the document is
followed properly.
This document is not part of any sales contract between
Thermo Fisher Scientific Inc. and a purchaser. This document
shall not govern or modify any Terms and Conditions of Sale,
which Terms and Conditions of Sale shall govern all
conflicting information between the two documents.
Legal Information
Note: Specifications, terms and pricing are subject to
change. Not all products are available in all countries. Consult
your local sales representative for details.
Warranty
Thermo Fisher Scientific warrants the operational safety and
functions of the biosafety cabinet only under the condition
that:
• The device is operated and serviced exclusively in
accordance with its intended purpose and as described
in these operating instructions
• The device is not modified
• Only original spare parts and accessories that have been
approved by Thermo Fisher Scientific are used
• Inspections and maintenance are performed at the
specified intervals
• An installation certification is performed prior to the initial
operation of the device and that a repeat certification is
performed on the occasion of all inspections and repairs.
The warranty is valid from the date of delivery of the device to
the operator.

2 | Introduction 1500 Series Class II, Type B2 Biological Safety Cabinet
Introduction
Thermo Fisher Scientific™ 1500 Series B2 Class II, Type B2
Biosafety Cabinet™ has been tested and certified in
accordance to NSF/ANSI 49, and is designed to protect the
user, the environment, and your research from harmful
substances and cross-contamination. This user’s manual
provides instructions on how to use the 1500 Series B2 most
effectively and safely.
The 1500 Series B2 offers a unique range of product features
that will enhance your safety and improve overall operating
efficiency. Should you have any questions on using this
product or need further explanation of any of its features,
contact Thermo Fisher Scientific technical support group at
the phone numbers listed on the following pages.
Postal address USA
Thermo Fisher Scientific Inc. (Asheville) LLC
401 Millcreek Road, Box 649 
Marietta, OH 45750
United States
Enquiries from North America, Latin America and Asia
Pacific
Phone: [phone]
Fax:[phone]
Email: [e-mail]
Intended Use
Correct Use:
The biosafety cabinet is a laboratory device for installation and
operation in microbiological and biotechnical laboratories of
biosafety levels 1, 2, and 3. It has been designed as a Class II
Biosafety Cabinet, Type B2, in accordance with NSF/ANSI
49.
Depending on the hazard level of the agents involved, the
operator must prepare in writing appropriate decontamination
procedures for the device and the accessories used in the
sample chamber.
Prior to the initial operation of the cabinet, the operator must
perform an installation test, or certification. According the
NSF, the test result must be documented by a test report.
The cabinet must only be used after it is in compliance with
the operating parameters as specified by Thermo Fisher
Scientific.
After any changes to the installation conditions and after any
modification to the technical system, the certification test
must be repeated and the test result must be documented in
a way that demonstrates all operating parameters are in
compliance with those specified by Thermo Fisher Scientific.
Incorrect Use:
To ensure optimum safety to the user and the environment,
the biosafety cabinet should not be used in laboratories that
do not comply with the requirements of biosafety levels 1, 2 or
3.
The device must not be operated as a Class II, Type B2
Biosafety Cabinet if:
• No repeat test is performed after changes to the
installation conditions or after modifications to the
technical system,
• The alarm system of the device has issued a failure
message and the cause for the failure has not been
repaired.
The alarm system must not be tampered with or disabled. 
If alarm system components have been removed or disabled
for service or repairs, the device must only be released for
operation after all alarm system components are confirmed to
be functioning properly.
The HEPA filters installed in the device are not capable of
separating gaseous substances. Therefore, avoid storing or
processing gases or gas-releasing substances in the device:
• Which in quantity or concentration are toxic,
• If a reduction with other substances may result in
hazardous toxic concentrations or formation of toxic
gases, that may form combustive or explosive mixtures in
combination with air.
Instruction of the
Operating Personnel
The operating instructions describe the biosafety cabinet
1500 Series B2, and applies to the models:
• 4-foot models 1510 / 1511 / 1515 / 1516 / 1517 / 1518
• 6-foot models 1560 / 1561 / 1562 / 1563 / 1564 / 1565
This biosafety cabinet has been manufactured in keeping with
the latest technological developments and has been tested
before delivery for its correct function. It may, however,
present potential hazards if it is not used according to the
intended purpose or outside of operating parameters.
Therefore, the following procedures must always be observed
to prevent accidents:

1500 Series Class II, Type B2 Biological Safety Cabinet Introduction | 3
• The biosafety cabinet should be operated by authorized
personnel who have been trained on the use and
maintenance of this product.
• For any operation of this device, it is recommended that
the operator prepares clear and concise written
instructions in the language of the operating and cleaning
personnel based on these operating instructions,
applicable safety data sheets, plant hygiene guidelines,
and technical regulations. Most importantly:
• which decontamination measures are to be applied
for the cabinet and accessories
• which protective measures apply while specific
agents are used
• which measures are to be taken in the case of an
accident.
• To ensure safety during the repair of the cabinet, servicing
of the equipment must be carried out by trained and
authorized personnel.

4 | Safety Precautions 1500 Series Class II, Type B2 Biological Safety Cabinet
Safety Precautions
In this manual, the following symbols and conventions are
used.
Below are important safety precautions that apply to this
product.
CAUTION: Do not position the unit so that it is
difficult to operate the main disconnect device.
CAUTION: Electrical outlets in the cabinet are
restricted to 5 amps (115 V models) or 2 amps
(230 V models) maximum current.
CAUTION: Do not use any detachable power
cord that is not adequately rated for the unit.
WARNING: Do not attempt to lift the Biosafety
Cabinet by the side panels, damage will occur.
Note: The Biosafety Cabinet should be certified
by a certification technician before its initial use.
The cabinet should be recertified whenever it is
relocated, serviced or at least annually thereafter.
Filter integrity and airflow performance should be
verified before using the cabinet.
WARNING: The Total Exhaust Biosafety Cabinet
is designed to be connected to an appropriate
exhaust system. Without verified inflow velocity,
the cabinet may NOT contain hazardous
particulate or gases. Do not attempt to operate it
when it is not connected to an appropriate
exhaust system that has been inspected by a
qualified certifier.
WARNING: Type B2 Biosafety Cabinets rely on
external blowers (usually on the roof of a building)
to exhaust 100% of the air entering the cabinet. If
the building exhaust blower fails or is mistakenly
turned OFF, the cabinet will not provide
containment of hazardous materials.
WARNING: Do not use the cabinet until the
problem has been corrected.
CAUTION: Only lift and support the cabinet at
the stainless steel sides and back. Damage will
occur if lifted or supported on the front edge.
CAUTION: Some internal components of the
Biosafety Cabinet may become contaminated
during operation of the unit. Only experienced
personnel competent in decontamination
procedures should decontaminate the cabinet
before servicing these components. If you have
any questions regarding certification agencies, or
need assistance in locating one, contact Thermo
Fisher Scientific.
CAUTION: DO NOT load more than 50 lbs. (23
Kg) in the work area. Exceeding this limit may
damage the work surface and its supports.
Excessive weight in the cabinet may increase the
risk of it overturning, or failure of hydraulic lift
stands, resulting in the cabinet and stand
overturning. If your application requires loading
more than 50 lbs., contact Thermo Fisher
Scientific.
CAUTION: Ensure that the cabinet is connected
to electrical service in accordance with local and
national electrical codes. Failure to do so may
create a fire or electrical hazard. Do not remove
or service any electrical components without first
disconnecting the Biosafety Cabinet from
electrical service.
CAUTION: Avoid the use of flammable gases or
solvents in the Biosafety Cabinet. Care must be
taken to ensure against the concentration of
flammable or explosive gases or vapors. An open
flame should NOT be used in the Biosafety
Cabinet. Open flames will disrupt airflow
patterns, burn the HEPA filter and/or damage the
filter’s adhesive. Gases under high pressure
should not be used in the Biosafety Cabinet, as
they may disrupt its airflow patterns.
CAUTION: HEPA filters only remove particulate
matter. Operations generating volatile toxic
chemicals or radionuclides must be evaluated
carefully.
CAUTION: The media of HEPA filters is fragile
and should not be touched. Avoid puncturing
either HEPA filter during installation or normal
operation. If you suspect that a HEPA filter has
been damaged, DO NOT use the cabinet;
contact a local certification agency or Thermo
Fisher Scientific.

1500 Series Class II, Type B2 Biological Safety Cabinet Safety Precautions | 5
CAUTION: The HEPA filters in the Biosafety
Cabinet will gradually accumulate airborne
particulate matter from the room and from work
performed in the cabinet. The rate of
accumulation will depend upon the cleanliness of
the room air, operating time and the nature of
work being done in the cabinet. The Filter Gauge
accurately displays the amount of filter life
remaining for the cabinet downflow filter.
CAUTION: Proper operation of the cabinet
depends largely upon its location and the
operator’s work habits. Consult the Installation
and Normal Operation sections of this manual for
further details.
CAUTION: Avoid direct exposure of plastic or
coated materials to ultraviolet (UV) radiation.
Never bypass the UV safety interlock that only
allows the UV light to work when the sash is
closed. When surface disinfecting the Biosafety
Cabinet:

Avoid splashing the disinfecting solution on skin
or clothing.

Ensure adequate ventilation.

Carefully follow the disinfectant’s safety
instructions.

Always dispose of disinfecting solutions in
accordance with local and national laws.
CAUTION: DO NOT allow disinfectants with
high concentrations of free chlorine to contact
the stainless steel components of the Biosafety
Cabinet for a long period of time. Free chlorine
will corrode stainless steel after extended
contact.
CAUTION: Biosafety Cabinets should be
decontaminated for any of the following reasons:

Before maintenance work requiring entry into
contaminated areas.

Before HEPA filter changes.

Before performing certification tests requiring
entry into contaminated areas.

Before relocating the cabinet.

Before changing research programs.

After the gross spill of biohazardous material or
toxic chemicals.

6 | Prerequisites 1500 Series Class II, Type B2 Biological Safety Cabinet
Prerequisites
Before you install the Biosafety Cabinet, you need to prepare
the site for installation. Examine the location where you intend
to install the cabinet. You must be certain that the area is level
and of solid construction. In addition, a dedicated source of
electrical power must be located near the installation site.
This section lists all requirements for:
•Space
• Clearance
•Location
• Exhaust
• Electrical
• Service Lines
Refer to Appendix C: Specifications, for complete Type B2
cabinet electrical and environmental conditions, specifications
and requirements.
Space Requirements
The dimensions for the different models are shown in
Appendix B: Dimensions.
Clearance Requirements
A minimum clearance of at least 6" (150 mm) is suggested on
the top and both sides of the cabinet for service.
Location Requirements
Note: The Biosafety Cabinet should be located away from
traffic patterns, doors, fans, ventilation registers, fume hoods
and any other air-handling devices that could disrupt its
airflow patterns. All windows in the room should remain
closed. Figure 1 shows the preferred location for the
Biosafety Cabinet.
Figure 1. Location Requirements
Exhaust System
Requirements
Note: The Exhaust Blower cannot run at its nominal flow rate
with the sash completely closed. If you choose to close the
sash completely to use the uv light for surface disinfection,
then the Exhaust Blower must be SHUT OFF, or its flow rate
reduced 90% or more for proper operation. The Biosafety
Cabinet has an optional en/contact circuit board that has dry
contacts that can be configured to signal the Exhaust Blower
to turn on when the Biosafety Cabinet blower is on. For
further information, contact Thermo Fisher Scientific product
service department. As an alternative, a remote electrical
switch for the Exhaust Blower can be installed near the
Biosafety Cabinet.
Before deciding on a location, examine it carefully to ensure
that it is compatible with the cabinet’s exhaust duct. The area
directly above the cabinet’s exhaust port should be clear of
structural elements, water and utility lines, or other fixed
obstructions. There should be enough clearance to allow for
the passage of a 10" PVC or stainless steel duct. Avoid
cabinet locations that require either an elbow directly on top
of the cabinet’s exhaust connection or an excessive number
of elbows to clear other items. For a further discussion of the
cabinet’s exhaust system requirements, go to Getting
Started.
CAUTION: Do not position the unit so that it is
difficult to operate the main disconnect device.

1500 Series Class II, Type B2 Biological Safety Cabinet Prerequisites | 7
Electrical Requirements
The Biosafety Cabinet models have the following electrical
requirements:
A dedicated outlet with an appropriate circuit breaker should
be located as close as possible to the right rear side of the
cabinet, at a height even with, or higher than, the top of the
bench or stand. Consult your local electrical codes for
properly rated circuit breakers. For safe operation the
dedicated outlet must provide the protective earthing ground
connection to the cabinet.
On 100 and 115 V AC models, both electrical outlets are
protected by a ground fault interrupter circuit (GFIC). Thermo
Scientific does not recommend plugging the Biosafety
Cabinet into a GFIC outlet.
Service Line Requirements
All utility service lines should be ¼" O.D., brass, copper, or
stainless steel, and equipped with an easily accessible 
shut-off valve. If the service line pressure exceeds 40 PSI, it
must be equipped with a pressure regulator to reduce the line
pressure.
The use of flammable gases or solvents should be avoided in
the Biosafety Cabinet. Open flame in the cabinet will disrupt
the laminar airflow in the cabinet and may damage the HEPA
filters. Flammable gases or solvents may reach explosive
concentrations in the cabinet or ductwork. If you feel that the
procedure requires the use of an open flame or flammable
materials, contact the institution’s safety office.
The use of air or gases under high pressure should be
avoided as they may seriously disrupt the airflow patterns in
the cabinet.
Table 1. Electrical Requirements
Model Requirements
1510, 1511 115 V AC, 60 Hz, 12 Amps
1515, 1516, 1517, 1518 230 V AC, 50/60 Hz, 6 Amps
1560, 1561 115 V AC, 60 Hz, 12 Amps
1562, 1563, 1564, 1565 230 V AC, 50 / 60 Hz, 6 Amps
CAUTION: Electrical outlets in the cabinet are
restricted to 5 amps (115 Volt models) or 2 amps
(230 Volt models) maximum current.
CAUTION: Do not use any detachable power
cord that is not adequately rated for the unit.

8 | Getting Started 1500 Series Class II, Type B2 Biological Safety Cabinet
Getting Started
Now that the installation is properly prepared, you are ready
to inspect, install, and certify the Biosafety Cabinet. This
chapter covers how to:
• Unpack and move the Biosafety Cabinet
• Install the cabinet
• Connect the electrical supply source
• Connect the service lines
• Connect to an exhaust system
• Arrange certification of the Biosafety Cabinet
Tools required for installation the Biosafety Cabinet include
two 1/2" wrenches, a flat-blade screwdriver, a #2 Phillips
screwdriver, and a carpenter’s level.
Note: The Biosafety Cabinet models weigh between 
400–700 lbs. (182-318 kg). The shipping pallet allows for
lifting with a mechanical lift truck or floor jack. If you must lift
the product manually, use at least six (6) persons and follow
safe-lifting guidelines.
Unpacking the Biosafety
Cabinet
1. Carefully remove the outer carton and inspect the cabinet
for damage that may have occurred in transit. If the
Biosafety Cabinet is damaged, notify the delivery carrier
immediately and retain the entire shipment intact for
inspection by the carrier.
Note: United States Interstate Commerce Commission rules
require that claims be filed with the delivery carrier within
fifteen (15) days of delivery.
Do not return goods without the prior authorization of Thermo
Fisher Scientific. Unauthorized returns will not be accepted.
If the cabinet was damaged in transit, you must file a claim
directly with the freight carrier. Thermo Fisher Scientific
Corporation and its dealers are not responsible for shipping
damages.
Do not discard the carton or packing material for the Biosafety
Cabinet until all of the components have been checked,
installed and tested.
2. The cabinet is secured to the pallet in two places on each
side. To access the nuts and bolts, remove the side
panels by removing and keeping the two Phillips screws
on both panels. Swing the front of each panel away from
the cabinet, and lift it straight up to remove the panel
from the cabinet.
3. While the side panels are off, the sash weight support
pins must be removed. Follow the two yellow strings to
the support pin and its clevis pin. Remove the clevis pins,
and pull the support pins straight back. Discard the pins
and yellow string. See Figure 2 below.
Figure 2. Unpacking the Biosafety Cabinet
Note: The side panels must be removed to access the
fasteners that secure the Biosafety Cabinet to the pallet.
Preparing the Biosafety
Cabinet for Operation
Installation instructions for the cabinet are attached to the
sash of the Biosafety Cabinet. If these instructions are missing
or unclear, contact Product Service at 1-888-213-1790. The
following are located in a box (see Figure 2 on previous page
and Figure 4 below) underneath the work surface:
CAUTION: Do not attempt to lift the Biosafety
Cabinet by the side panels, damage will occur.
Yellow String 
(to Left End Pins)
Parts Box
(Figure 3)
Yellow
String
Clevis Pin Support Pin
Yellow
String

1500 Series Class II, Type B2 Biological Safety Cabinet Getting Started | 9
Figure 3. Parts box
If you did not receive one or more of the components listed for
the cabinet, or if any of the components are damaged,
contact Thermo Fisher Scientific immediately for further
instructions.
Moving and Lifting the
Cabinet
Move the cabinet, attached to its pallet, by using a floor jack,
or a furniture dolly underneath the unit. DO NOT move the
cabinet by tilting it onto a hand truck.
When lifting the cabinet DO NOT lift the cabinet in the middle
front area of the hull. Lifting here may bend or distort the
bottom of the cabinet, causing damage to the unit.
Installing the Biosafety
Cabinet on an Existing
Work Surface
Note: The Biosafety Cabinet is very top heavy. Use caution
when lifting or moving it.
When installing the Biosafety Cabinet onto an existing work
surface or bench top, ensure that the structure can safely
support the combined weight of the cabinet and any related
equipment. The work surface should be at least as wide as
the cabinet and 31" (787 mm) deep to properly support the
unit. A hole or notch may be cut in the supporting surface in
the right front corner to accommodate the optional drain
valve.
Installing the Cabinet on a
Thermo Fisher Scientific
Base Stand
Thermo Scientific offers accessory Base Stands in a variety of
configurations to suit your particular needs. Stands can be
ordered with adjustable telescoping legs or with a manually or
electrically adjustable hydraulic lift.
Telescoping Base Stands
Adjust the leg height before installing the cabinet on top of the
stand. The height can be set in 1" intervals between 27.5" to
34.5" (699 mm to 876 mm), providing a Cabinet work surface
height from 30" to 37" (762 mm to 940 mm). The Base
Stands for each width Cabinet are listed in Ta b le 2.
1. Before positioning the Telescoping Base Stand in its final
location, adjust the height. A plastic bag containing the
mounting bolts for the Biosafety Cabinet is shipped with
the base stand. Remove and save it.
2. Select the height of the stand and slide four (4) leg
extensions into base stand corner posts and attach with
2 ¼" long bolt, flat washer, lock washer and nut. Ensure
that the same height hole is selected for each leg.
Tighten the leg bolts securely. See Figure 4.
• User’s Manual Thumb Drive
• Drain Valve Assembly and fasteners
• Power Cord
• SmartPort Accessories (optional)
Parts Box
Underneath the
work surface
Table 2. Telescoping Base Stands
Width Base Stand w/Feet Model #
4’ 3730402
6’3730602

10 | Getting Started 1500 Series Class II, Type B2 Biological Safety Cabinet
Figure 4. Adjusting the height of Telescoping Base
Stand
Figure 5. Leveling the Telescoping Base Stand
Exhaust System
Connections
The Total Exhaust Biosafety Cabinets are a “Type B2
cabinet,” meaning they direct their HEPA filtered exhaust air
out of the laboratory.
• Justify the type of work is appropriate for the cabinet
Type B2
• Exhaust system must be reliable, maintained, frequently
inspected and preferably redundant
• Exhaust termination must be distant from other building
air intake systems to prevent the re-entrainment of
volatile chemicals
Note: The Exhaust Blower cannot run at its nominal flow rate
with the sash completely closed. If you choose to close the
sash completely to use the UV light for surface disinfection,
then the Exhaust Blower must be SHUT OFF, or its flow rate
reduced 90% or more for proper operation. The Biosafety
Cabinet control board has dry contacts that can be
configured to signal the Exhaust Blower to turn ON when the
Biosafety Cabinet blower is ON. For further information,
contact Thermo Fisher Scientific product service department.
As an alternative, a remote electrical switch for the Exhaust
Blower can be installed near the Biosafety Cabinet.
The exhaust connection is a sealed Exhaust System from the
top of the cabinet to the remote blower. The Exhaust Systems
should be dedicated to a single cabinet. The cabinet is
equipped with an exhaust flow alarm, such that an alarm
sounds, and the cabinet blower shuts off in the event of
insufficient exhaust flow.
If your research involves the use of toxic compounds or
volatile materials, contact your facility’s safety officer or
Thermo Fisher Scientific to ensure that your cabinet and its
CAUTION: Only lift and support the cabinet at the
stainless steel sides and back. Damage will occur
if lifted or supported on the front edge. Move the
base stand into its final location. Using a
carpenter’s level, adjust each leveling foot until the
stand is level in both planes as shown in Figure 5.
You are now ready to lift the Biosafety Cabinet
onto its stand.
Remove these four nuts and
bolts to change the height of
the base stand
Turn the four leveling
feet to level the base
stand to the floor.
Level the base stand at
these three positions
WARNING: The Total Exhaust Biosafety Cabinet
is designed to be connected to an appropriate
exhaust system. Without verified inflow velocity,
the cabinet may NOT contain hazardous
particulate or gases. Do not attempt to operate it
when it is not connected to an appropriate
exhaust system that has been inspected by a
qualified certifier.
WARNING: Type B2 Biosafety Cabinets rely on
external blowers (usually on the roof of a building)
to exhaust 100% of the air entering the cabinet. If
the building exhaust blower fails or is mistakenly
turned OFF, the cabinet will be pressurized,
resulting in a flow from the work area into the
laboratory. It is imperative that the following be
considered in a risk assessment:

1500 Series Class II, Type B2 Biological Safety Cabinet Getting Started | 11
exhaust system are compatible with the materials you will be
working with.
Exhaust System
Requirements
The cabinet exhaust stack is sized to accept 10" (254 mm)
stainless steel ductwork. The exhaust system’s blower should
be sized to handle the exhaust volume of each cabinet, as
shown in the Appendix E: Quick Chart. The exhaust system
must maintain a static pressure equal to or greater than the
system pressure, plus 1.5" (38 mm) of water for the Biosafety
Cabinet. In order to get the maximum life out of the cabinet’s
exhaust filter, the system needs to be able to operate at an
additional 2" (51 mm) of vacuum, compared to the initial total
system pressure.
Note: The exhaust system may be fitted with a backdraft
damper (Part #3858800) to prevent the reversing of airflow in
the system.
Connecting the Biosafety
Cabinet to Utility Service
Lines
Note: Some models have a solenoid valve connected to the
service valve on the right side, rear position. The solenoid
prevents gas from flowing to the service valve when the unit
blower is off. It is the only service valve position that can be
fitted with a solenoid valve. Connect the gas service to the
solenoid valve.
The service lines (if any) should be connected to the tube
fitting(s) on the outside of the liner wall as shown in Figure 6.
To install the tubing, follow these steps:
1. Ensure that the tubing is ¼" O.D., soft metal, and that the
end has been completely deburred.
2. Route the tubing from the rear of the cabinet, ensuring
that it will line up with the slot in the back of the side
panel. The slot is located from 8 ¾" to 11 ¼" (222 mm to
288 mm) from the bottom of the cabinet.
Note: Make sure that the tube routing will not contact any
electrical wires. DO NOT loop service line tubing within the
side panels of the cabinet.
3. Make sure that the nut on the tube fitting is loose, but do
not remove it. Make sure the tube ferrule is in the fitting.
4. Push the tube into the fitting until it is properly seated.
The tube will go approximately ¾" (19 mm) into the fitting.
5. Tighten the tube fitting nut hand tight and then, using a 
7/16” wrench, tighten it at least ¾ turn more.
6. Close the service valve in the Biosafety Cabinet and then
slowly open the shutoff valve on the service valve. Test all
fittings for leakage. Tighten the tube nut slightly if needed.
Figure 6. Service lines connectivity
Using the SmartPortTM
Cord & Cable Portal
There must be enough clearance to pass the cord or cable
between the Thermo Fisher Scientific exterior dress panel and
any obstruction.
Note: Some SmartPort B2 components and the cord or
cable passing through it may become contaminated during
use of the cabinet. Ensure all potentially contaminated
components are surface decontaminated before handling or
removal from the cabinet.
1. Remove the grommet from the liner side wall. Remove
the solid sealing plug from the body of the portal by either
pressing it through from the outside, or by carefully
inserting a spatula or similar device between the sealing
plug and the body of the portal, and prying the plug out.
2. Locate the pass-thru universal sealing plug, which is
included in the parts box located underneath the work
surface during shipment.
3. Pass the cord or cable through the body of the portal,
and then through one of the plugs that has been cut for
cord or cable use, then through the grommet, as shown
in Figure 7.
Note: Select a hole in the sealing plug that is slightly smaller
than the cord or cable, to create a proper seal. This will also
Tube 
fitting nut
Tube 
fitting

12 | Getting Started 1500 Series Class II, Type B2 Biological Safety Cabinet
help minimize movement of the cord or cable if it is
accidentally pulled during use.
Figure 7. Pass the cord or cable through the body
of the portal
4. Position the cord or cable as it will be used in the cabinet,
and then push the plug back into the body of the portal
until it seats in the portal. Reinstall the grommet.
Installing the Drain Valve
In order to prevent damage during shipping, the drain valve
assembly has not been installed. If desired, the valve should
be installed after the cabinet is in its final location.
To install the valve assembly, follow these steps:
Note: The work surface is heavy. Use caution when handling
it.
1. Locate the Drain Valve components and installation
hardware. The components and hardware are shipped in
a single bag inside the parts box, underneath the work
surface.
2. Lift the work surface out of the Biosafety Cabinet by lifting
on the knobs at the front of the work surface. Steady the
work surface while pulling it straight out the front of the
cabinet.
3. Using a putty knife, remove and discard the stainless
steel cover that is sealed over the drain mounting holes.
Scrape out remaining sealant that is around the holes.
4. Apply a light coating of silicone sealant (user supplied) to
the mounting surface of the drain assembly. Attach the
drain assembly under the bottom of the cabinet as
shown in Figure 8. Wipe off any excess sealant from the
cabinet bottom. Ensure that the center drain hole is
unobstructed.
5. Make sure the drain valve is in the closed position.
6. Reinstall the work surface.
7. Allow the silicone sealant to cure for at least eight hours
before exposing it to liquid.
Note: The drain valve assembly attaches to the underside of
the cabinet bottom.
Figure 8. Drain valve assembly
Initial Certification
Prior to use, a qualified certifier should certify all Biosafety
Cabinets. Under normal operating conditions, the Biosafety
Cabinet should be re-certified at least annually and when
relocated or serviced. The certifier should perform the
following tests, as recommended in ANSI/NSF Standard
Number 49 in effect when the cabinet was manufactured:
• Downflow Velocity Profile Test
• Inflow Velocity Test
• Airflow Smoke Patterns
• HEPA Filter Leak Test
• Site Installation Assessment Tests
• Vibration Test*
• Noise Level Test*
• Lighting Intensity Test*
*These tests are user comfort related tests and may be
omitted at the user’s or certifier’s discretion.
If you have any questions regarding certification agencies or
need assistance in locating one, contact Thermo Scientific at
1-800-438-4851.
Note: Service with costs: Unless certification was expressly
called for in the specification, quotes and/or purchase order,
the cost for this on-site testing is to be paid by the customer.
Apply a light coat of
silicone sealant to
this surface of the
connector, aligning
the three holes in
the connector with
the three holes in
the Biosafety
Cabinet liner.

1500 Series Class II, Type B2 Biological Safety Cabinet Performance Features | 13
Performance Features
All 1500 Series B2 Biosafety Cabinets operate using the
following principles:
• Filtration and retention of particulates by High Efficiency
Particulate Air (HEPA) filter(s)
• Laminar airflow
• Directional airflow
The major components in a Biosafety Cabinet are:
• The HEPA filter(s) or optional ULPA filters
• The motor/blower to force air through the cabinet
• Cabinet air intakes (grilles), ductwork and air balance
controls
HEPA Filters
HEPA filters are disposable, dry-type particulate filters. The
filter material or media is typically made of borosilicate
microfibers formed into a thin sheet, in a process similar to the
production of paper. This sheet is folded, or pleated to
increase its surface area. The pleats are typically held in place
by beads of glue that add rigidity to the media pack. The pack
is then set into a frame, and sealed as shown in Figure 9.
The HEPA filter manufacturer establishes the efficiency of the
filter by challenging it with an aerosol of known particle size.
The number of particles that penetrate the filter are quantified,
and this establishes the efficiency of the filter. Thus, the filters
used in the Biosafety Cabinet cabinets are at least 99.99%
efficient in removing particles 0.3 micron.
Note: The HEPA filter media is very fragile. DO NOT touch the
media. If you think the media of a HEPA filter is damaged, DO
NOT USE THE CABINET. Have the HEPA filter integrity tested
by a certifier before using the cabinet.
Note: HEPA Filters are only effective against particulate
material. Gases will pass through the filter.
Figure 9. HEPA Filter
ULPA Filters
Optional ULPA filters may be used to replace the standard
HEPA filters in the Thermo Fisher Scientific Biosafety
Cabinets. ULPA filters have the same properties as described
above except they are rated at least 99.999% efficient in
removing particles 0.1-0.2 or 0.2-0.3 micron.
Laminar Airflow
Laminar airflow is defined as the movement of a body of air in
a single direction, with a uniform velocity. In practice, the
laminar downflow of air in the cabinet captures any aerosol
generated in the work area of the cabinet, and directs it to the
HEPA filters. In order to be true laminar downflow, a number
of individual downflow velocity test points (The Downflow
Velocity Profile) must be +/- 16 feet per minute (0.08 m/s) of
the average of all the test points. This is illustrated in
Figure 10.
Polyurethane seal
Between media
pack and filter frame
Filter 
frame
Filter media pack 
(A continuous sheet
of filter media)

14 | Performance Features 1500 Series Class II, Type B2 Biological Safety Cabinet
Figure 10. Laminar Airflow
Directional Airflow
Directional airflow also plays a key role in Biosafety Cabinet
performance. Air is drawn into the front of the cabinet at the
front grille. This “curtain” of air makes it more difficult for
aerosols to escape out of the work area of the cabinet and
into the outside environment. This airflow is often calculated
and referred to as the Inflow Volume or Average Inflow
Velocity. This is illustrated in Figure 11.
Figure 11. Directional Airflow
Motor/Blower
The supply motor/blower assembly pulls room air into the top
of the cabinet, and circulates it internally as downflow. The
downflow air is split into two separate streams. One path
flows into the front intake grille, where it joins the inflow air, as
the inflow air and front portion of the downflow pass under the
work surface toward the rear of the cabinet. The other
downflow path flows to the rear intake grille. As the rear
portion of the downflow passes through the rear intake grille,
it joins the mixed inflow and front portion of the downflow and
all of the inflow and all of the downflow passes up inside the
rear wall as shown in Figure 12. The combined inflow and
downflow is drawn through the exhaust filter, through the
external exhaust duct and out of the building. The downflow
motor in the cabinet is an electronically commutated motor
(ECM). The ECM is a brushless DC motor that includes its
own power supply to convert the incoming alternating current
to direct current, as well as its own microprocessor to control
and measure the motor’s operation. The motor utilizes
SmartFlow to deliver a consistent volume of downflow air,
throughout the life of the cabinet.
Figure 12. Motor/Blower Assembly
Airflow Sensor
An airflow sensor, located above the Exhaust HEPA filter,
constantly monitors the flow of exhaust air out of the cabinet.
If the exhaust falls below a safe level, the control board turns
off the cabinet blower, and sounds an audible and visual
alarm. This prevents the escape of hazardous material from
the front of the cabinet, in the event of an exhaust system
failure. The airflow sensor reading is shown on the display as
Inflow in feet per minute (FPM) or meters per second (M/S).

1500 Series Class II, Type B2 Biological Safety Cabinet Performance Features | 15
Cabinet Grilles, Ductwork
and Air Balance Controls
The location, size, and pattern of the grilles at the front and
rear of the work area affect cabinet containment and
performance.
Note: Do not block or obstruct the grilles of the Biosafety
Cabinet.
The supply intake of the Biosafety Cabinet conveys the air to
the downflow blower and then from the blower through the
work area as downflow. The positive pressure rigid downflow
plenum of the Biosafety Cabinet is designed to deliver a more
uniform airflow, optimizing filter loading and operational life.
Ultraviolet (UV) Lamp
The optional UV lamp generates a primary wavelength of light
of 254 nm. A secondary emission is in the visible (blue)
wavelength, resulting in the characteristic blue color while
operating. UV light at this wavelength is biocidal, primarily by
creating thymine dimers in DNA. These dimers prevent the
correct transcription of the DNA into RNA, resulting in cellular
death or viral inactivation. In order to be effective, the UV light
must directly strike the nucleic acid, and its effectiveness can
be diminished or negated by dissolved proteins or metals, or
by other UV-opaque substances protecting the target 
nucleic acid.
Because of its limitations, UV light should be used as an
adjunct to good surface disinfection practices. In order to get
optimum performance from the UV light, it should be replaced
after 6,000 hours of operation or less, and the exterior surface
of the lamp should be kept clean and free of dust.
Note: The Biosafety Cabinet records the number of hours of
operation of the UV light. You can program in the number of
hours (in 100-hour increments) it will operate before a
replacement message is displayed.
Note: UV irradiation is absorbed by the tempered safety glass
of the sash. Independent research has shown that the level of
UV irradiation on the outside of the cabinet’s sash is equal to
background radiation levels.
Note: The UV sensitivity of a target organism varies,
depending on the UV output of the lamp, the genus and
species of the organism, the medium the agent is suspended
in, etc. Contact the Health and Safety Officer at your facility for
UV light use and recommendations.