Thermo Scientific thermoscientific Vanquish Pumps C User manual

thermoscientific
Vanquish
Pumps C, Pumps F
VC-Pxx, VF-Pxx
Operating Manual
4820.4405-EN Revision 4.0 •December 2019

Copyright © 2019 Thermo Fisher Scientific Inc. All rights reserved.
Original Operating Manual
The hardware descriptions in this manual revision refer to devices VC-P10-A-01, VC-P20-A-01,
VC-P21-A-01, VC-P32-A-01, VC-P33-A-01, VC-P40-A-01, VF-P10-A-01, VF-P20-A, VF-P32-A-01.
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All other trademarks are property of Thermo Fisher Scientific and its subsidiaries.
Disclaimer
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of Thermo Fisher Scientific Inc.
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Contacting Us
Pumps C, Pumps F ( VC-Pxx, VF-Pxx)
Operating Manual
Page 3

Contacting Us
Page 4 Pumps C, Pumps F ( VC-Pxx, VF-Pxx)
Operating Manual

Contents
Pumps C, Pumps F ( VC-Pxx, VF-Pxx)
Operating Manual
Page 5
Contents
1 Using this Manual ...................................................................... 11
1.1 About this Manual ................................................................................................12
1.2 Conventions..........................................................................................................13
1.2.1 Safety Messages....................................................................................... 13
1.2.2 Special Notices and Informational Notes ................................................. 13
1.2.3 Typographical Conventions ...................................................................... 14
1.3 Reference Documentation ...................................................................................15
2 Safety......................................................................................... 17
2.1 Safety Symbols and Signal Words.........................................................................18
2.1.1 Safety Symbols and Signal Words in This Manual .................................... 18
2.1.2 Observing this Manual ............................................................................. 18
2.1.3 Safety Symbols on the Device .................................................................. 19
2.1.4 Rating Plate .............................................................................................. 19
2.2 Intended Use ........................................................................................................20
2.3 Safety Precautions................................................................................................21
2.3.1 General Safety Information...................................................................... 21
2.3.2 Qualification of the Personnel ................................................................. 22
2.3.3 Personal Protective Equipment................................................................ 22
2.3.4 Electrical Safety Precautions .................................................................... 23
2.3.5 General Residual Hazards......................................................................... 24
2.3.6 In Case of Emergency ............................................................................... 25
2.4 Solvent and Additive Information ........................................................................26
2.4.1 General Compatibility .............................................................................. 26
2.4.2 Allowed pH Ranges................................................................................... 27
2.4.3 Allowed Concentrations ........................................................................... 27
2.4.4 Further Information ................................................................................. 28
2.5 Compliance Information.......................................................................................29
3 Device Overview ........................................................................ 31
3.1 Pump Features .....................................................................................................32
3.2 Operating Principle...............................................................................................33
3.3 Interior Components ............................................................................................34
3.4 Piston Seal Wash System......................................................................................35
3.5 Purge/Sensor Unit ................................................................................................36

Contents
Page 6 Pumps C, Pumps F ( VC-Pxx, VF-Pxx)
Operating Manual
3.6 Mixing System ......................................................................................................37
3.7 Leak Detection......................................................................................................38
3.8 Operation .............................................................................................................39
4 Unpacking .................................................................................. 41
4.1 Unpacking.............................................................................................................42
4.2 Scope of Delivery..................................................................................................44
5 Installation................................................................................. 45
5.1 Safety Guidelines for Installation .........................................................................46
5.2 Installing the Device .............................................................................................47
5.3 Site Requirements ................................................................................................49
5.3.1 Power Considerations .............................................................................. 49
5.3.2 Power Cord............................................................................................... 50
5.3.3 Condensation ........................................................................................... 50
5.4 Accessing the Interior Components .....................................................................51
5.5 Setting Up the Hardware......................................................................................52
5.5.1 System Arrangement................................................................................ 52
5.5.2 Connecting the Device ............................................................................. 53
5.5.3 Connecting the Power Cord ..................................................................... 55
5.6 Setting Up the Flow Connections .........................................................................56
5.6.1 General Information and Guidelines........................................................ 56
5.6.2 Guiding Capillaries and Tubing Through the System................................ 57
5.6.3 Connecting Fittings, Capillaries, and Tubing ............................................ 59
5.6.4 Guiding Liquids to Waste ......................................................................... 64
5.6.5 Connecting the Solvent Lines ................................................................... 64
5.6.6 Installing Solvent Shut Off Valves............................................................. 69
5.7 Seal Wash System.................................................................................................71
5.7.1 Choosing the Seal Wash Liquid ................................................................ 71
5.7.2 Setting Up the Seal Wash System ............................................................ 72
5.7.3 Flushing the Seal Wash System ................................................................ 78
5.8 Connecting the Pump and Autosampler ..............................................................80
5.9 Turning On the Device..........................................................................................81
5.10 Setting Up the Device in the Software .................................................................82

Contents
Pumps C, Pumps F ( VC-Pxx, VF-Pxx)
Operating Manual
Page 7
6 Operation................................................................................... 83
6.1 Introduction to this Chapter.................................................................................84
6.2 Safety Guidelines for Operation ...........................................................................85
6.3 Control Elements..................................................................................................86
6.3.1 Keypad...................................................................................................... 86
6.3.2 Status Indicators....................................................................................... 88
6.4 Power On/Off Control ..........................................................................................91
6.5 Use of Solvents and Additives ..............................................................................92
6.6 Preparing the Device for Operation .....................................................................95
6.7 Important Operating Parameters.........................................................................97
6.8 Purging the Pump .............................................................................................. 103
6.8.1 Automatic Purging.................................................................................. 104
6.8.2 Manual Purging ...................................................................................... 105
6.9 Optimizing the Performance of the Device ....................................................... 110
6.9.1 General Guidelines ................................................................................. 110
6.9.2 Installing Solvent Shut Off Valves........................................................... 110
6.10 Shutting Down the Device ................................................................................. 113
6.10.1 Short-Term Shutdown (Interruption of Operation) ............................... 113
6.10.2 Long-Term Shutdown............................................................................. 114
6.10.3 Restart after Long-Term Shutdown........................................................ 116
7 Maintenance and Service ......................................................... 117
7.1 Introduction to Maintenance and Service......................................................... 118
7.2 Safety Guidelines for Maintenance and Service................................................ 119
7.3 General Rules for Maintenance and Service ..................................................... 121
7.4 Routine and Preventive Maintenance............................................................... 122
7.4.1 Internal Maintenance............................................................................. 122
7.4.2 Maintenance Plan .................................................................................. 122
7.4.3 Flushing the Pump.................................................................................. 124
7.4.4 Cleaning or Decontaminating the Device............................................... 126
7.4.5 Predictive Performance.......................................................................... 127
7.5 Seal Wash System.............................................................................................. 129
7.5.1 Testing the Seal Wash System for Leakage ............................................ 130
7.5.2 Replacing the Seal Wash Lines ............................................................... 131
7.5.3 Replacing the Seal Wash Detector ......................................................... 134

Contents
Page 8 Pumps C, Pumps F ( VC-Pxx, VF-Pxx)
Operating Manual
7.6 Pump Head ........................................................................................................ 136
7.6.1 Overview of Pump Head Parts ............................................................... 137
7.6.2 Replacing the Pump Head ...................................................................... 138
7.6.3 Replacing the Pistons ............................................................................. 144
7.6.4 Cleaning the Piston................................................................................. 146
7.6.5 Replacing the Piston Seals or Support Ring............................................ 147
7.6.6 Recommendations for New Piston Seals................................................ 151
7.6.7 Replacing the Seal Wash Seals ............................................................... 154
7.6.8 Replacing the Pump Head Seals ............................................................. 157
7.6.9 Testing the Piston Seals for Leakage ...................................................... 159
7.7 Check Valves...................................................................................................... 162
7.7.1 Replacing the Inlet Check Valves............................................................ 162
7.7.2 Replacing the Outlet Check Valves......................................................... 164
7.7.3 Cleaning the Check Valves...................................................................... 167
7.8 Mixing System ................................................................................................... 168
7.8.1 Testing the Static Mixer for Permeability............................................... 168
7.8.2 Replacing the Static Mixer or Capillary Mixer ........................................ 169
7.9 Solvent Lines and Solvent Line Filters................................................................ 171
7.9.1 Emptying the Solvent Lines .................................................................... 171
7.9.2 Replacing Solvent Lines .......................................................................... 172
7.9.3 Replacing the Solvent Line Filter ............................................................ 175
7.10 Replacing the Purge Valve Knob........................................................................ 177
7.11 Testing the Pump for Leakage (General Test) ................................................... 179
7.12 Replacing the Main Power Fuses....................................................................... 182
7.13 Updating the Device Firmware.......................................................................... 184
7.14 Replacing the Doors........................................................................................... 186
7.15 Transporting or Shipping the Device ................................................................. 188
7.15.1 Preparing the Device for Transport........................................................ 188
7.15.2 Transporting the Device to a New Location ........................................... 189
7.15.3 Shipping the Device................................................................................ 190
7.16 Replacing the Slide-In Module........................................................................... 191
7.16.1 Removing the Slide-In Module ............................................................... 191
7.16.2 Returning the Slide-In Module ............................................................... 192
7.16.3 Installing the Slide-In Module ................................................................ 193
7.16.4 Setting Up the Slide-In Module .............................................................. 195

Contents
Pumps C, Pumps F ( VC-Pxx, VF-Pxx)
Operating Manual
Page 9
8 Troubleshooting....................................................................... 197
8.1 General Information about Troubleshooting .................................................... 198
8.2 Messages ........................................................................................................... 200
8.3 Testing the Pump for Leakage ........................................................................... 208
8.4 Resolving Liquid Leaks ....................................................................................... 209
8.5 Pressure Issues or Shifts in Retention Time....................................................... 210
8.5.1 Pressure Not Zero with Open Purge Valve............................................. 210
8.5.2 Checking the Compression Values ......................................................... 210
8.5.3 Testing the Static Mixer for Permeability............................................... 212
8.6 Removing Persistent Air Bubbles....................................................................... 213
9 Pump-Specific Information....................................................... 215
9.1 Introduction to this Chapter.............................................................................. 216
9.2 Quaternary Pump and Dual Pump..................................................................... 217
9.2.1 Interior Components .............................................................................. 218
9.2.2 Operating Principle................................................................................. 221
9.2.3 Dwell Volume of the Pump .................................................................... 222
9.2.4 Setting the Solvent Composition............................................................ 223
9.2.5 Synchronizing the Injection Time with the Pump Strokes...................... 224
9.2.6 Dual Pump Specifics ............................................................................... 225
9.3 Binary Pump ...................................................................................................... 228
9.3.1 Interior Components .............................................................................. 228
9.3.2 Operating Principle................................................................................. 230
9.3.3 Dwell Volume of the Pump .................................................................... 231
9.3.4 Setting the Solvent Composition............................................................ 232
9.4 Isocratic Pump ................................................................................................... 233
9.4.1 Interior Components .............................................................................. 233
9.4.2 Operating Principle................................................................................. 234
10 Modifying the Pump for Specific Applications .......................... 235
10.1 Gradient Delay Volume, Dwell Volume and Ripple ........................................... 236
10.1.1 Available Mixing Systems ....................................................................... 237
10.1.2 Exchanging the Mixing System............................................................... 239
10.2 Normal-Phase Compatible Solvents and Additives ........................................... 249
10.2.1 Modifying the Pump and Autosampler .................................................. 249
10.2.2 Considerations with Normal-Phase Compatible Solvents and Additives251

Contents
Page 10 Pumps C, Pumps F ( VC-Pxx, VF-Pxx)
Operating Manual
11 Specifications ........................................................................... 253
11.1 Performance Specifications............................................................................... 254
11.1.1 Quaternary Pump and Dual Pump ......................................................... 254
11.1.2 Binary Pump ........................................................................................... 256
11.1.3 Isocratic Pump........................................................................................ 258
11.2 Physical Specifications....................................................................................... 260
12 Accessories, Consumables and Replacement Parts ................... 261
12.1 General Information.......................................................................................... 262
12.2 Ship Kit............................................................................................................... 263
12.2.1 Binary Pump ........................................................................................... 263
12.2.2 Dual Pump.............................................................................................. 265
12.2.3 Isocratic Pump........................................................................................ 266
12.2.4 Quaternary Pump................................................................................... 267
12.3 Optional Accessories ......................................................................................... 269
12.3.1 Optional Accessories (Miscellaneous).................................................... 269
12.3.2 Optional Mixing Systems and Inline Filters ............................................ 270
12.4 Consumables and Replacement Parts ............................................................... 273
12.4.1 Maintenance Kits ................................................................................... 273
12.4.2 Pump Heads and Components............................................................... 277
12.4.3 Solvents and Wash Systems ................................................................... 280
12.4.4 Mixing Systems....................................................................................... 282
12.4.5 Miscellaneous Parts ............................................................................... 282
12.4.6 Interface Cables and Power Cords ......................................................... 283
13 Appendix.................................................................................. 285
13.1 Compliance Information.................................................................................... 286
13.1.1 Declarations of Conformity .................................................................... 286
13.1.2 WEEE Compliance .................................................................................. 287
13.1.3 FCC Compliance...................................................................................... 287
13.1.4 Manual Release History.......................................................................... 287
13.2 Digital I/O .......................................................................................................... 288
Index........................................................................................ 291