Thermo Scientific Microm HM 430 User manual

Thermo Scientific Sliding Microtome Microm HM 430
Microm International GmbH
part of Thermo Fisher Scientific
Otto-Hahn-Str. 1A
69190 Walldorf / Germany
387 756 -English
1
Thermo Scientific
Sliding Microtome
Microm HM 430
Instruction Manual

Thermo Scientific Sliding Microtome Microm HM 430
Microm International GmbH
part of Thermo Fisher Scientific
Otto-Hahn-Str. 1A
69190 Walldorf / Germany
387 756 -English
2

Thermo Scientific Sliding Microtome Microm HM 430
Microm International GmbH
part of Thermo Fisher Scientific
Otto-Hahn-Str. 1A
69190 Walldorf / Germany
387 756 -English
3
CERTIFICATION
Thermo Fisher Scientific certifies that this instrument has been tested and
checked carefully. Its technical data was verified before shipment to be in
accordance with the published specifications.
The instrument complies with applicable international safety regulations.
WARRANTY
This Thermo Fisher Scientific product is warranted against defects in
material and workmanship for a period of 1 year. Parts which prove to be
defective during the warranty period will be repaired or replaced free of
charge by Thermo Scientific. No other warranty is expressed or implied.
Unauthorized modification or repair by third party persons will void the
warranty.
The warranty will expire in case of improper or wrong use of the instrument
and in case the warning and precautionary messages are not observed.
Thermo Scientific is not liable for any occurring damage.
Errors and ommissions excepted. Subject to amendment and improvement
without further notice.
This instruction manual will be supplied together with each instrument.
Further copies can be ordered at the nearest Thermo Scienitific sales office
by giving the serial number of the instrument, the number of the instruction
manual and the date of issue.
This instruction manual is available in the following languages:
German: 387755
English: 387756
French: 387757
Spanish: 387758

Thermo Scientific Sliding Microtome Microm HM 430
Microm International GmbH
part of Thermo Fisher Scientific
Otto-Hahn-Str. 1A
69190 Walldorf / Germany
387 756 -English
4
INTENDED USE
Dear valued customer,
Thank you for buying a Thermo Scientific instrument.
Before operating the instrument, please read these instructions carefully to
familiarize you with its proper operation and functions.
The Thermo Scientific Sliding Microtome Microm HM 430 is a highly efficient
sliding microtome for paraffin sectioning in routine.
Only skilled or specially trained personnel must operate the microtome, i.e.
clamping the specimen, trimming, sectioning and taking off the sections from
the instrument. The listed and marked safety measures as well as the
regulations of your respective lab must be strictly observed.
Serial No. ...................................................................................
Please check the serial no. on the type plate of your instrument and enter
this number here.
Instruction Manual no. 387756
Issue: March 02nd, 2011
Microm International GmbH
part of Thermo Fisher Scientific
Otto-Hahn-Str. 1A
69190 Walldorf –Germany
Phone:[phone]
Fax:[phone]
E-Mail: [e-mail]
Internet: www.thermoscientific.com

Thermo Scientific Sliding Microtome Microm HM 430
Microm International GmbH
part of Thermo Fisher Scientific
Otto-Hahn-Str. 1A
69190 Walldorf / Germany
387 756 -English
5
Intended use
Table of contents
Safety Precautions
Part 1 Introduction
1-1 Description of the Sliding Microtome HM 430
1-2 Technical Data HM 430
Part 2 Operating Instructions
2-1 Setting up the microtome
2-2 Cutting movement
2-3 Locking the sledge
2-3-1 Additional Ballast for Sledge
2-4 Specimen feed
2-4-1 Setting section thickness
2-4-2 Coarse feed
2-4-3 Fine feed, manual
2-4-4 Fine feed, automatic
2-4-5 Moving the automatic feed position
2-5 Specimen clamping
2-5-1 Standard specimen clamp
2-5-2 Standard specimen clamp
2-5-3 Universal cassette clamp
2-6 Changing the the specimen clamp
2-7 Knife carrier
2-7-1 Blade carrier for disposable blades
2-8 Standard equipment
2-9 Additional equipment (optional)
2-9-1 Disposable blade holder
2-9-2 Specimen clamps
2-9-3 Height adjustment plates
2-9-4 Microtome knives
2-9-5 Additional accessories
Part 3 Theory of operation
3-1 Cutting movement
3-2 Specimen coarse feed
3-3 Specimen clamping and specimen orientation
3-4 Knife carrier
3-5 Microtome with fast freezing unit

Thermo Scientific Sliding Microtome Microm HM 430
Microm International GmbH
part of Thermo Fisher Scientific
Otto-Hahn-Str. 1A
69190 Walldorf / Germany
387 756 -English
6
Part 4 Working with the microtome
4-1 Preparation and orientation
4-2 Coarse feed
4-3 Sectioning instructions
4-3-1 Condition of the knife edge
4-3-2 Clean knife surfaces
4-4 How to avoid malfunctions
4-4-1 Preparation of the specimen
4-4-2 Specimen temperature
4-4-3 Tightening the clamping screws
4-4-4 Selection of the knife
4-4-5 Adjustment of the knife
4-4-6 Cutting speed
4-4-7 Trimming
Part 5 Maintenance
Part 6 Cleaning and care
Part 7 Conditions for the transportation of the instrument
7-1 Taking back the instrument for repair or routine maintenance
7-2 Disposal of the instrument after final shutdown

Thermo Scientific Sliding Microtome Microm HM 430
Microm International GmbH
part of Thermo Fisher Scientific
Otto-Hahn-Str. 1A
69190 Walldorf / Germany
387 756 -English
7
SAFETY PRECAUTIONS
WARNING SIGNALS AND SYMBOLS
The installation and routine use of the Sliding Microtome HM 430 is easy and safe if the instructions in this
manual are being observed.
Note:
Special instructions regarding operation of the instrument.
Warning:
Special precautionary measures to prevent damage to equipment. For a long lifetime of the
equipment, please observe these instructions carefully.
Caution –general danger spot:
The instruction manual must strictly be observed whenever this symbol is visible on the
instrument.
Hazard of hand injuries:
Due to moving parts in connection also with the microtome knife, a danger area arises, which
might lead to hand injuries in case of non-compliance with the safety features of the microtome
and when disregarding the instruction manual.
Biohazard:
Warning of biological danger.
Radioactivity:
Warning of radioactive danger.
Separate taking back of electrical and electronic instruments in the countries of the
European Union:
This is to be applied in the countries of the European Union and other European countries with a
separate collecting system within the waste management.
This product, being an electro and/or electronic instrument, must be treated separately within the
waste management process (WEEE).

Thermo Scientific Sliding Microtome Microm HM 430
Microm International GmbH
part of Thermo Fisher Scientific
Otto-Hahn-Str. 1A
69190 Walldorf / Germany
387 756 -English
8
SAFETY PRECAUTIONS
A T T E N T I O N !
The operator's safety is affected, when the instrument is not operated in accordance with
the instruction manual.
Please observe the following general precautions during operation of this instrument.
Failure to comply with these precautions violates safety standards and the intended use of
the instrument. Thermo Scientific is not liable for misuse of the instruments and failure to
comply with basic safety requirements.
CARE IN USING MICROTOME KNIFE
To diminish the danger of being injured by the knife or blade, use the knife guard when adjusting specimen
and knife. If possible, the specimen should be clamped in before the knife is inserted into the knife holder.
Before changing the knife holder, always remove blade or knife! Unused knives should always be kept in a
knife case. Never place the knife with the cutting edge upwards. Never try to catch a dropping knife!! Never
check the sharpness of the cutting edge with your fingers. The cutting edge is extremely sharp!
DANGER IN EXPLOSIVE ENVIRONMENT
The instrument must not be operated in the presence of flammable gases. Moreover, the instrument must
not be exposed to conditions whereby dangerous gas concentrations can occur.
HAZARD OF RADIOACTIVE RADIATION
When working with radioactive specimens observe all applicable radiation safety procedures. When working
with radioactive contaminated material, appropriate safety and disinfection measures must be carried out.
According to the rules and regulations concerning the handling of radioactive contaminated material of the
respective laboratory, safety clothing (e.g. particle mask, gloves, protective shoe covers) must be worn.
Radioactive contaminated waste must be disposed of according to the respective regulations.

Thermo Scientific Sliding Microtome Microm HM 430
Microm International GmbH
part of Thermo Fisher Scientific
Otto-Hahn-Str. 1A
69190 Walldorf / Germany
387 756 -English
9
HAZARD OF INFECTION
Specimens used during the intended operation of the instrument might potentially be infectious. For this
reason, it is recommended to observe the general laboratory regulations concerning protection against
danger of infection.
Information on decontamination media, their use, dilution and effective range of application can be read in
the Laboratory Biosafety Manual : 1984 of the World Health Organization.
When working with infectious specimens observe all applicable safety procedures. When working with
infectious material, appropriate safety and disinfection measures must be carried out. According to the rules
and regulations concerning the handling of infectious material of the respective laboratory, safety clothing
(e.g. particle mask, gloves, protective shoe covers) must be worn. Infectious waste must be disposed of
according to the respective regulations.

Thermo Scientific Sliding Microtome Microm HM 430
Microm International GmbH
part of Thermo Fisher Scientific
Otto-Hahn-Str. 1A
69190 Walldorf / Germany
387 756 -English
10
Fig. 1