Thermo Scientific Finnpipette User manual

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Finnpipette®Digital
Single Channel
Multichannel
Instructions for Use
Bedienungsanleitung
Guide d´utilisation
Instrucciones de uso
取扱説明書
User Manual

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Product specifications are subject to change without prior notice. Finnpipette®and
Finntip®are registered trademarks of Thermo Fisher Scientific Oy.
Wir behalten uns das Recht auf unangekündigte Änderungen der Produktspezifikationen
vor. Finnpipette®und Finntip®sind eingetragene Warenzeichen der Fa. Thermo Fisher
Scientific Oy.
Les spécifications du produit sont sujettes à modification sans avis préalable. Finnpipette®
et Finntip®sont des marques déposées de Thermo Fisher Scientific Oy.
Las especificaciones del producto pueden cambiar sin previo aviso. Finnpipette®y
Finntip® son marcas registradas de Thermo Fisher Scientific Oy.
製品仕様は事前の予告なく変更されることがあります。フィンピペット®とフィ
ンチップ®は、サーモフィッシャーサイエンティフィックの登録商標です。

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English
DeutschFrançaisEspañol
日
本
語
PRODUCT DESCRIPTION 4
PACKAGE 4
SAFETY 5
PIPETTE OPERATION 6
PIPETTING TECHNIQUES 6
CALIBRATION AND ADJUSTMENT 7
MAINTENANCE 10
TROUBLE SHOOTING 13
SPARE PARTS 58-64
INHALT
目次
PRODUKTBESCHREIBUNG 14
VERPACKUNG 14
SICHERHEIT 15
HANDHABUNG DER FINNPIPETTE 16
PIPETTIER-TECHNIKEN 16
KALIBRIERUNG UND JUSTIERUNG 18
WARTUNG 21
STÖRUNGEN BEHEBEN 24
ERSATZTEILE UND ZUBEHÖR 58-64
DESCRIPTION 25
CONDITIONNEMENT 25
CONSIGNES DE SÉCURITÉ 26
UTILISATION DE LA PIPETTE 27
CONSEILS DE PIPETAGE 27
CALIBRAGE ET RÉGLAGE 29
MAINTENANCE 32
ERREURE ET CAUSES 36
PIÈCES DÉTACHÉES 58-64
DESCRIPCIÓN DEL PRODUCTO 37
PRESENTACION 37
SEGURIDAD 38
FUNCIONES 39
TÉCNICAS DE PIPETEO 39
CALIBRACIÓN Y AJUSTE 40
MANTENIMIENTO 43
PROBLEMAS Y SOLUCIONES 46
PIEZAS DE RECAMBIO 58-64
製品について 47
パッケージ 47
安全にお使いいただくために 48
ピペットの操作 49
ピペッティングテクニック 49
キャリブレーション 50
メンテナンス 53
トラブルシューティング 56
保証規定 56
パーツ及び付属品 58-64
CONTENTS
SOMMAIRE
CONTENIDO

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PRODUCT DESCRIPTION
The Finnpipette Digital is an autoclavable digital pipette. It operates on the air displacement
principle (i.e. an air interface) and uses detachable, disposable tips.
The adjusted delivery volume is displayed digitally on a readout window on the handle.
The ten different models of Finnpipette Digital pipettes cover a volume range from 0.2 µl to 10 ml.
Order No. Volume Range Color code Finntip
4500000 0.2 µl to 2 µl pink 10, 20, 50, Flex 10
4500010 0.5 µl to 10 µl grey 10, 20, 50, Flex 10
4500020 0.5 µl to 10 µl grey 250 Univ., 200 Ext, 300, Flex 200, Flex 300
4500080 2 µl to 20 µl orange 250 Univ., 200 Ext, 300, Flex 200, Flex 300
4500100 5 µl to 50 µl orange 250 Univ., 200 Ext, 300, Flex 200, Flex 300
4500110 10 µl to 100 µl yellow 250 Univ., 200 Ext, 300, Flex 200, Flex 300
4500090 20 µl to 200 µl yellow 250 Univ., 200 Ext, 300, Flex 200, Flex 300
4500120 100 µl to 1000 µl blue 1000, 1000 Ext, Flex 1000, Flex 1200
4500060 1 ml to 5 ml green 5 ml
4500070 2 ml to 10 ml red 10 ml, Flex 10 ml Ext
The seven different models of Finnpipette Digital Multichannel pipettes cover a volume range
from 0.5 µl to 300 µl.
Order No. Channel Volume Range Color code Finntip
4510000 8 0.5 µl to 10 µl grey 10, 20, 50, Flex 10
4510010 12 0.5 µl to 10 µl grey 10, 20, 50, Flex 10
4510020 8 5 µl to 50 µl orange 250 Univ., 200 Ext, 300, Flex 200, Flex 300
4510030 8 50 µl to 300 µl yellow 300, 300 Flex
4510040 12 5 µl to 50 µl orange 250 Univ., 200 Ext, 300, Flex 200, Flex 300
4510050 12 50 µl to 300 µl yellow 300, 300 Flex
4510070 16 5 µl to 50 µl green 50
DIGITAL DISPLAY
The adjusted delivery volume is clearly indicated in the
large digital display on the handle.
RAW MATERIALS
The Finnpipette Digital is made of mechanically durable and chemically resistant materials
which allow repeated autoclaving of the complete pipette at 121°C.
DESCRIPTION OF TIPS
Finntips are recommended for use with the Finnpipette Digital.
They are made of natural colour polypropylene, generally regarded as the only contamination
free material suitable for tips. Finntips are also autoclavable (121°C).
PACKAGE
The Finnpipette Digital is shipped in a specially designed package containing the following
items:
1. The Finnpipette
2. Service tool
3. Maintenance pliers
4. Instructions for Use
5. Calibration certificate / Warranty certificate
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SAfETy
Cautions are marked with this symbol .
Intended use
The intended use of the device is to transfer liquids in the volume range of 0,2 µl to 10 ml.
The Finnpipette Digital pipette and Finntips are designed as a component of an analyzing system
for an end user, who is responsible for validating the system to ensure reliable and safe results.
Damage to health
Follow general procedures for hazard prevention and safety instructions; e.g. wear protective
clothing, eye protection and gloves.
For use and waste disposal of hazardous (e.g. radioactive and potentially infectious)
material, follow the safety instructions and general laboratory practice.
The pipette and tips are not intended for in vivo use. Do not use the pipette for pipetting any
liquid to be injected into a human body.
Do not eject the tip towards anybody.
The Finnpipette Digital is to be used by trained personnel with required laboratory skills. The
instructions for use must be read prior to and during the use of the device (pipette and tip). The
Finnpipette Digital pipette can be used between +4°C and +40°C.
Incorrect dispensing results
Performance may vary due to:
a. pipetting method (forward pipetting technique recommended)
b. temperature (air, liquid, vessel, pipette, and tip)
c. pressure
d. humidity
e. operator, e.g. thumb movement, pipetting angle
f. liquid density, viscosity and vapor pressure
g. type of tip
If the pipetting performance is critical to the outcome of a specific application, the result has
to be assured with an alternative test, and if this is not an option, by duplicate testing.
The possibility of an incorrect volume delivery during pipetting cannot be entirely mitigated.
To avoid inaccurate dispensing and/or leakage, check that the tip is properly attached to
the pipette.
When rotating the volume adjustment button, do not exceed the volume range of
the pipette.
Otherwise the pipette may be damaged or affect pipetting performance.
Pipetting performance cannot be guaranteed if the tip is reused.
Choose only a tip and pipette with matching color coding.
Disposal of the Materials
Follow laboratory and country-specifi c procedures for the disposal of biohazardous or radioactive
waste. Refer to local regulations for the disposal of infectious material.
Warning The tips can be potentially infectious. Dispose of all used disposable tips as
biohazardous waste.
Warning Decontaminate the pipette before disposal.
Waste disposal according to local instructions taking into account legislation in place. For more
information, please contact us at info.pipettes@thermofisher.com or your local Thermo
Fisher Scientific representative.
Regarding the original packaging and packing materials, use the recycling operators known
to you.

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Figures:
A = Ready position
B = First stop
C = Second stop
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PIPETTE OPERATION
SETTING THE DELIVERY VOLUME
1. Set the delivery volume using the push button on the top of the pipette.
To increase the delivery volume, turn the push button counterclockwise.
To decrease the delivery volume, turn it clockwise.
2. Make sure that the desired delivery volume clicks into place and that
the digits are completely visible in the display window.
3. Do not set volumes outside the pipette´s specified volume range.
Using excessive force to turn the push button outside the range may jam
the mechanism and eventually damage the pipette.
TIP EJECTION
To help eliminate the risk of contamination, each pipette is fitted with a tip
ejector system.
The tip ejector system consists of a soft-touch tip ejector and specially designed
gearing mechanism. To release the tip, point the pipette at suitable waste
receptacle and press the tip ejector with your thumb.
SAFETY LABEL
You can mark the pipette application your initials the
calibration date, etc.on the safety label.
Remove the clear plastic window on the edge closest to
the push button (use the service tool that comes with the
pipette, or a screwdriver). Mark the adhesive label with a
felt-tipped or other pen and snap the window back in place.
PIPETTING TECHNIQUES
Push and release the push button slowly at all times
particularly when working with high viscosity liquids. Never
allow the push button to snap back.
Make sure that the tip is firmly attached to the tip cone.
Check for foreign particles in the tip.
Before you begin your actual pipetting work, fill and empty
the tip 2-3 times with the solution that you will be pipetting.
Hold the pipette in an upright position while aspirating liquid. The grippy should rest on your
index finger. Make sure that the tips, pipette and solution are at the same temperature.
FORWARD TECHNIQUE
Fill a clean reagent reservoir with the liquid to be dispensed.
1. Depress the push button to the first stop.
2. Dip the tip under the surface of the liquid in the
reservoir to a depth of about 1 cm and slowly
release the push button. Withdraw the tip from the
liquid touching it against the edge of the reservoir
to remove excess liquid.
3. Deliver the liquid by gently depressing the push
button to the first stop. After a delay of about one
second, continue to depress the push button all
the way to the second stop. This action will empty
the tip.
4. Release the push button to the ready position.If necessary, change the tip and continue pipetting.
REVERSE TECHNIQUE
The reverse technique is suitable for dispensing liquids that have a high viscosity or a tendency
to foam easily. The technique is also recommended for dispensing very small volumes. Fill a
clean reagent reservoir with the liquid to be dispensed.

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1. Depress the push button all the way to the second stop.
2. Dip the tip under the surface of the liquid in the
reservoir to a depth of about 1 cm, and slowly
release the push button.
This action will fill the tip. Withdraw the tip from
the liquid touching it against the edge of the
reservoir to remove excess liquid.
3. Deliver the preset volume by gently depressing the
push button to the first stop.
Hold the push button at the first stop. Some liquid
will remain in the tip and this should not be
included in the delivery.
4. The remaining liquid should either be discarded with the tip or pipetted back into the container.
REPETITIVE TECHNIQUE
The repetitive technique offers a rapid and simple procedure for repeated delivery of the same
volume. Fill a clean reagent reservoir with the liquid to be dispensed.
1. Depress the push button all the way to the second stop.
2. Dip the tip under the surface of the liquid in the
reservoir to a depth of about 1 cm, and slowly
release the push button. This action will fill the tip.
Withdraw the tipfrom the liquid touching against
the edge of the reservoir to remove excess liquid.
3. Deliver the preset volume by gently depressing the
push button to the first stop.
Hold the push button at the first stop. Some liquid
will remain in the tip and this should not be included
in the delivery.
4. Continue pipetting by repeating steps 2 and 3.
PIPETTING OF HETEROGENEOUS SAMPLES
(deproteinization in blood glucose determination, for example)
Use steps 1 and 2 of the forward technique to fill the tip with blood.
Wipe the tip carefully with a dry clean tissue.
1. Immerse the tip into the reagent and depress the
push button to the first stop, making sure the tip is
well below the surface.
2. Release the push button slowly to the ready
position. This will fill the tip. Keep the tip in the
solution.
3. Depress the push button to the first stop and
release slowly. Keep repeating this procedure until
the interior wall of the tip is clear.
4. Finally, depress the push button all the way to the second stop to completely empty the tip.
CALIBRATION AND ADJUSTMENT
All Finnpipettes are factory calibrated and adjusted to give the volumes as specified with
distilled or deionized water using the forward pipetting technique. It should be noted that the
use of other pipetting techniques may affect the calibration results. The pipettes are constructed
to permit re-adjustment for other pipetting techniques or liquids of different temperature and
viscosity.
DEVICE REQUIREMENTS AND TEST CONDITIONS
An analytical balance must be used. The scale graduation value of the balance should be chosen
according to the selected test volume of the pipette:
Volume range readable graduation
under 10 µl 0.00 1 mg
10-100 µl 0.01 mg
above 100 µl 0.1 mg
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Test liquid: Water, distilled or deionized, “grade 3” water conforming ISO 3696. Tests are done
in a draft-free room at a constant (±0.5°C) temperature of water, pipette and air between 15°C
to 30°C. The relative humidity must be above 50%. Especially with volumes under 50 µl the
air humidity should be as high as possible to reduce the effect of evaporation loss. Special
accessories, such as the evaporation trap, are recommended.
PROCEDURE TO CHECK CALIBRATION
The pipette is checked with the maximum volume (nominal volume) and with the minimum
volume. A new tip is first pre-wetted 3-5 times and a series of ten pipettings is done with
both volumes. A pipette is always adjusted for delivery (Ex) of the selected volume. Use of
forward pipetting technique is recommended. The maximum permissible errors are designed for
forward method. It is recommended to calibrate all channels of the multichannel pipette, but a
suitable method is also to calibrate only the edge channels. Adjustment check prior calibration is
done using one of the middle channels. It is recommended to use the ISO8655 calibration limits
presented in the following tables or user-defined specifications.
Procedure:
1. Do 10 pipettings with the minimum volume.
2. Do 10 pipettings with the maximum volume.
3. Calculate the inaccuracy (A) and imprecision (cv) of both series.
4. Compare the results to the limits.
If the calculated results are within the selected limits, the adjustment of the pipette is correct.
ISO8655 calibration specifications
Thermo Scientific Finnpipette Digital specifications according to ISO8655 standard.
Finnpipette Digital Single Channel
Range Volume Inaccuracy Imprecision
µl µl % s.d. µl cv%
0,2-2 µl 2 ±0.080 ±4 0.040 2.0
0.2 ±0.080 ±40 0.040 20.0
0,5-10 µl 10 ±0.120 ±1.2 0.080 0.8
1 ±0.120 ±12 0.080 8.0
2-20 µl 20 ±0.20 ±1.0 0.10 0.5
2 ±0.20 ±10.0 0.10 5.0
5-50 µl 50 ±0.50 ±1.0 0.20 0.4
5 ±0.50 ±10 0.20 4.0
10-100 µl 100 ±0.80 ±0.8 0.30 0.3
10 ±0.80 ±8.0 0.30 3.0
20-200 µl 200 ±1.60 ±0.8 0.60 0.3
20 ±1.60 ±8.0 0.60 3.0
100-1000 µl 1000 ±8.0 ±0.8 3.0 0.3
100 ±8.0 ±8.0 3.0 3.0
1-5 ml 5000 ±40.0 ±0.8 15.0 0.3
1000 ±40.0 ±4.0 15.0 1.5
2-10 ml 10000 ±60.0 ±0.6 30.0 0.3
2000 ±60.0 ±3.0 30.0 1.5
Finnpipette Digital Multichannel
Range Channel Volume Inaccuracy Imprecision
µl µl % s.d.µl cv%
0.5-10 µl 8, 12 10 ±0.24 ±2.4 0.16 1.6
1 ±0.24 ±24 0.16 16
5-50 µl 8, 12, 16 50 ±1.0 ±2.0 0.4 0.8
5 ±1.0 ±20 0.4 8.0
50-300 µl 8, 12 300 ±8.0 ±2.7 3.0 1.0
50 ±8.0 ±16 3.0 6
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Manufacturer specification limits
Finnpipette Digital Single Channel
Range Volume Inaccuracy Imprecision Factory
µl µl % s.d. µl cv% calibration tip
0.2-2 µl 2 ±0,050 ±2,50 0,040 2,00 10
0,2 ±0,024 ±12,00 0,020 10,00
0.5-10 µl, micro 10 ±0,100 ±1,00 0,050 0,50 10
1 ±0,025 ±2,50 0,020 2,00
0.5-10 µl 10 ±0,100 ±1,00 0,080 0,80 250 Univ.
1 ±0,035 ±3,50 0,030 3,00
2-20 µl 20 ±0,20 ±1,00 0,08 0,40 250 Univ.
2 ±0,06 ±3,00 0,05 2,50
5-50 µl 50 ±0,30 ±0,60 0,15 0,30 250 Univ.
5 ±0,15 ±3,00 0,125 2,50
10-100 µl 100 ±0,80 ±0,80 0,20 0,20 250 Univ.
10 ±0,30 ±3,00 0,10 1,00
20-200 µl 200 ±1,2 ±0,60 0,4 0,20 250 Univ.
20 ±0,36 ±1,80 0,14 0,70
100-1000 µl 1000 ±6,0 ±0,60 2,0 0,20 1000
100 ±1,0 ±1,00 0,6 0,60
1-5 ml 5000 ±25,0 ±0,50 10,0 0,20 5 ml
1000 ±8,0 ±0,80 3,0 0,30
2-10 ml 10000 ±50,0 ±0,50 20,0 0,20 10 ml
2000 ±20,0 ±1,00 6,0 0,30
Finnpipette Digital Multichannel
Range Channels Volume Inaccuracy Imprecision Factory
µl µl % s.d. µl cv% calibration tip
0.5-10 µl 8, 12, 16 10 ±0,240 ±2,40 0,160 1,60 10
1 ±0,120 ±12,00 0,080 8,00
5-50 µl 8, 12, 16 50 ±0,75 ±1,50 0,35 0,70 250 Univ.
5 ±0,25 ±5,00 0,10 2,00
50-300 µl 8, 12 300 ±3,0 ±1,00 0,9 0,30 300
50 ±2,3 ±4,60 0,75 1,50
ADJUSTMENT
Adjustment is done only for one volume. The recommended
adjustment volume is the minimum volume or 10% of the maximum
volume. Multichannel pipettes adjustment check prior calibration
is done using one of the middle channels.
1. Place the service tool into the openings of the calibration nut at
the top of the handle.
2. Turn the service tool clockwise to increase, or counterclockwise
to decrease the volume.
3. After adjustment check the calibration according to the
instructions above.
FORMULAS FOR CALCULATING RESULTS
Conversion of mass to volume
V = (w + e) x Z V = volume (µl)
w = weight (mg)
e = evaporation loss (mg)
Z = conversion factor for mg/µl conversion

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Evaporation loss can be significant with low volumes. To determine mass loss, dispense water
to the weighing vessel, note the reading and start a stopwatch. See how much the reading
decreases during 30 seconds (e.g. 6 mg = 0.2 mg/s).
Compare this to the pipetting time from taring to reading. Typically pipetting time might be 10
seconds and the mass loss is 2 mg (10 s x 0.2 mg/s) in this example. If an evaporation trap or lid
on the vessel is used the correction of evaporation is usually unnecessary.
The factor Z is for converting the weight of the water to volume at test temperature and pressure.
A typical value is 1.0032 µl/mg at 22°C and 95 kPa. See the conversion table on page 57.
Inaccuracy (systematic error)
Inaccuracy is the difference between the dispensed volume and the selected volume of a pipette.
A = V - V0A = inaccuracy
V = mean volume
V0 = nominal volume
Inaccuracy can be expressed as a relative value: A% = 100% x A / V0
Imprecision (random error)
Imprecision refers to the repeatability of the pipettings. It is expressed as standard deviation (s)
or coefficient of variation (cv)
s = standards deviation
v = mean volume
n = number of measurements
Standard deviation can be expressed as a relative value (CV) CV = 100% x S / V
MAINTENANCE
When the Finnpipette Digital is not in use, make sure it is stored in an upright position. We
recommend a Finnpipette stand for this purpose.
The part # refer to exploded views beginning at page 58.
SHORT-TERM CHECKING
The pipette should be checked at the beginning of each day for dust and dirt on the outside
surfaces of the pipette.
Particular attention should be paid to the tip cone. No other solvents except 70 % ethanol should
be used to clean the pipette.
LONG-TERM MAINTENANCE
Annual pipette usage varies significantly among laboratories, and the types of substances being
aspirated and dispensed also vary tremendously among laboratories. In order to maintain proper
care of the pipette, it is the laboratory’s responsibility to develop a proper maintenance program
that meets its specific needs and requirements.
The servicing procedure starts with the disassembly of the pipette.
DISASSEMBLING 0.2-50 µl PIPETTES
1. Press the tip ejector.
2. Insert the maintenance pliers under the ejector bar to release the
tip ejector.
3. Remove the tip cone by pressing with maintenance pliers.
4. Pull out the piston and the spring.
5. Keep the tip cone vertically and push out with
piston the rest of the piston assembly.
Then keep the tip cone upside down and tap all
parts from tip cone.
Remember keep all parts in order on table for
reassembly.
6. Clean the piston, the piston spring and the
O-rings with a dry napless cloth.
7. Check the tip cone for foreign particles.
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