Thermo Scientific IM448 User manual

IM-448-3 June 2012
Thermo Scientific
Medilite
Microcentrifuges
Instruction Manual
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IM-448-3 printed in June 2012.
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Thermo Scientific Medilite Microcentrifuges i
T
Chapter 1 Introduction ............................................................................................................................1-1
Characteristics ..........................................................................................................1-2
Technical Data .........................................................................................................1-3
Directives, Standards and Guidelines .......................................................................................1-4
Mains Supply ...........................................................................................................1-4
Rotor Selection .........................................................................................................1-5
Chapter 2 Installation ..............................................................................................................................2-1
Receive the Unit ......................................................................................................2-2
Prepare the Installation Site ......................................................................................2-2
Verify Power Configuration .....................................................................................2-2
Moving the Unit .....................................................................................................2-3
Chapter 3 Operation ................................................................................................................................3-1
Warnings and Cautions ...........................................................................................3-2
Operating Controls ..................................................................................................3-2
Loading ...................................................................................................................3-2
Starting a Run .........................................................................................................3-3
Stopping a Run .......................................................................................................3-3
Chapter 4 Maintenance .......................................................................................................................... 4-1
Care and Cleaning ...................................................................................................4-2
Chemical Compatibility Chart .............................................................................. A-1
Contact Information ................................................................................................ B-1
Table of Contents

Thermo Scientific Medilite Microcentrifuges ii
P
Preface
Before starting to use the centrifuge, read through this instruction manual carefully and follow the
instructions.
The information contained in this instruction manual is the property of Thermo Fisher Scientific; it is
forbidden to copy or pass on this information without explicit approval.
Failure to follow the instructions and safety information in this instruction manual will result in the
expiration of the sellers warranty.
Scope of Supply
If any parts are missing, please contact your nearest Thermo Fisher Scientific representative.
Intended Use
This centrifuge is a laboratory product designed to separate components by generation of relative
centrifugal force. The centrifuge is to be used for separating materials of different density or particle
size suspended in a liquid.
Maximum sample density at maximum speed: 1.2
Article number Quantity Check
Centrifuge Medilite 1
Rotor 1
IM448 CD with instruction manual 1
This symbol refers to general hazards.
WARNING means that injuries or material damage or contamination could occur.
CAUTION means that material damage could occur.
g
cm3
-----------

PAccident Prevention
iii Medilite Microcentrifuges Thermo Scientific
Accident Prevention
WARNING If a hazardious situation occurs, turn off the power supply to the centrifuge
and leave the area immediately.
WARNING If the centrifuge is used in a manner nor specified in this manual, the
protection provided by the centrifuge may be impaired.
Prerequisite for the safe operation of the Sorvall Medilite is a work environment in
compliance with standards, directives and trade association safety regulations and proper
instruction of the user.
The safety regulations contain the following basic recommendations:
•Maintain a radius of at least 30 cm around the centrifuge.
•Implementation of special measures which ensure that no one can approach the
centrifuge for longer than absolutely necessary while it is running.
•The mains plug must be freely accessible at all times. Pull out the power supply plug or
disconnect the power supply in an emergency.
Remaining risk: Inproper use can cause damages, contamincation, and inju-
ries with fatal consequences.

P Precautions
Thermo Scientific Medilite Microcentrifuges iv
Precautions
In order to ensure safe operation of the Sorvall Medilite, the following general safety
regulations must be followed:
•The centrifuge should be operated by trained specialists only.
•The centrifuge is to be used for its intended use only.
•While handling centrifuge, rotor, and samples you must wear laboratory clothing (e.g.
gloves).
•Plug the centrifuge only into sockets which have been properly grounded.
•Do not move the centrifuge while it is running.
•Do not lean on the centrifuge.
•Use only rotors and accessories for this centrifuge which have been approved by Thermo
Fisher Scientific. Exceptions to this rule are commercially available glass or plastic
centrifuge tubes, provided they have been approved for the speed or the RCF value of
the rotor.
•Do not use rotors which show any signs of cracks.
•Use only with rotors which have been properly installed. Follow the instructions in
section “Rotor Installation” on page 4-2.
•Use only with rotors which have been loaded properly.
•Never overload the rotor.
•Never open the door until the rotor has come to a complete stop.
•Never use the centrifuge if parts of it are damaged or missing.
•Do not touch the electronic components of the centrifuge or alter any electronic or
mechanical components.
•Please observe the safety instructions.
Please pay particular attention to the following aspects:
•Location: well-ventilated environment
•Place the centrifuge on a clean, dry surface, to make certain that the suction feet at the
bottom grip the surface firmly.
•Especially when working with corrosive samples (salt solutions, acids, bases), the
accessory parts and rotor chamber have to be cleaned carefully.
•Always balance the samples.

PPrecautions
vMedilite Microcentrifuges Thermo Scientific
Centrifuging hazardous substances:
•Do not centrifuge explosive or flammable materials or substances which could react
violently with one another.
•The centrifuge is neither inert nor protected against explosion. Never use the centrifuge
in an explosion-prone environment.
•Do not centrifuge inflammable substances.
•Do not centrifuge toxic or radioactive materials or any pathogenic micro-organisms
without suitable safety precautions.
When centrifuging microbiological samples from the Risk Group II (according to the
Biosafety Manual" of the World Health Organization (WHO)), aerosol-tight biological
seals have to be used.
For materials in a higher risk group, extra safety measures have to be taken.
•If toxins or pathogenic substances have gotten into the centrifuge or its parts,
appropriate disinfection measures have to be taken (see “Disinfection” on page 5-3).
•Highly corrosive substances which can cause material damage and impair the
mechanical stability of the rotor, should only be centrifuged in corresponding protective
tubes.
Remaining risk: Inproper use can cause damages, contamincation, and inju-
ries with fatal consequences.
Remaining risk: Inproper use can cause damages, contamincation, and inju-
ries with fatal consequences.

Thermo Scientific Medilite Microcentrifuges 1-1
1
Introduction
Contents
•“Characteristics” on page 1-2
•“Technical Data” on page 1-3
•“Directives, Standards and Guidelines” on page 1-4
•“Mains Supply” on page 1-4
•“Rotor Selection” on page 1-5

1Introduction
Characteristics
1-2 Medilite Microcentrifuges Thermo Scientific
Characteristics
The unit consists of:
•A baseplate with three rubber suction cup pads for stability.
•A brushless AC motor is attached to the cabinet. The motor is thermally protected in
accordance with UL and CSA specifications.
•A six or twelve place plastic 45° angle rotor.
•A 30 minute timer (with hold function).
•A viewport in the cover for easy speed verification.

1 Introduction
Technical Data
Thermo Scientific Medilite Microcentrifuges 1-3
Technical Data
The technical data of the Thermo Scientific Medilite is listed in the following table.
Table 1-1. Technical data Thermo Scientific Medilite
Feature Value
Environmental conditions -Indoor use only
-Altitudes of up to 2,000 m above sea level
-max. relative humidity 80 % up to 31°C; decreasing
linearly up to 50% relative humidity at 40°C
Permissible ambient temperature during operation +5°C to +40°C +5°C to +40°C
Permissible ambient temperature during storage and
shipping
+2°C to +50°C +2°C to +50°C
Overvoltage category II II
Pollution degree 2 2
Heat dissipation 220-240V 120V
274BTU/h 290BTU/h
IP 20 20
Max running time 30min 30min
Speed nmax 2700rpm 3100rpm
Maximum RCF value at nmax 920x g 1228x g
Maximum kinetic energy <285Nm <285Nm
Noise level at maximum speed < 65dB (A) < 65dB (A)
Dimensions
Hight
Hight, cover open
Width
Depth
241mm
457mmm
292mm
375mm
9.5in
18in
11.5in
14.75in
Weight 5.7kg 12.5lbs

1Introduction
Directives, Standards and Guidelines
1-4 Medilite Microcentrifuges Thermo Scientific
Directives, Standards and Guidelines
Mains Supply
The following table contains an overview of the electrical connection data for the Thermo Scientific
Medilite. This data is to be taken into consideration when selecting the mains connection socket.
Table 1-2. Directives, Standards and Guidelines
Tension /
Frequency Marked Produced and inspected according to the following standards and guidelines
220-240V
50Hz/60Hz,
CE
2006/42/EC Machine Directive
2004/108/EC EMC Directive
2006/95/EC Low Voltage Directive
EN 61010-1, 2nd Edition
EN 61010-2-020, 2nd Edition
120V 50Hz cCSAus: UL Std. No. 61010-1 (2nd Edition)
CAN/CSA-C22.2 No. 61010-1-04
CAN/CSA-C22.2 No. 61010-2-020-09
IEC 61010-2-020 2nd Edition
Table 1-3. Electrical connection data of theThermo Scientific Medilite
Cat. Mains
voltage Frequency Rated current
Power-
consumption Equipment fuse Building fuse
449 Medilite
6-place rotor
220-240 V 50 / 60 Hz 0.6 A 80 W 1.25A, slow blow 16 AT
448 Medilite
6-place rotor
120 V 50 / 60 Hz 1.2 A 85 W 2.5A, slow blowr 15 AT
459 Medilite
12-place rotor
220-240 V 50 / 60 Hz 0.6 A 80 W 1.25A, slow blow 16 AT
458 Medilite
12-place rotor
120 V 50 / 60 Hz 1.2 A 85 W 2.5A, slow blow 15 AT

1 Introduction
Rotor Selection
Thermo Scientific Medilite Microcentrifuges 1-5
Rotor Selection
The Thermo Scientific Medilite is supplied with a rotor.
Two rotors are available to choose from.
For more information visit our website at: www.thermoscientific.com/centrifuge.
6-Place Rotor 47430
12-Place Rotor 47620
Aeroshield 10 mL sealed with cap and o-ring 2087
Tube Size (mm)
O.D. x Length Min. 7 x100
O.D.x Length
Maximum 6 tubes (in 6 or 12 places)
16x133 or 17x120
O.D.x Length
Maximum 12 tubes
16x100
Maximum Volume
6 place 90 mL (6x15 mL)
12 place 130 mL (12x10 mL)

Thermo Scientific Medilite Microcentrifuges 2-1
2
Installation
Contents
•“Receive the Unit ” on page 2-2
•“Prepare the Installation Site” on page 2-2
•“Verify Power Configuration ” on page 2-2
•“Moving the Unit ” on page 2-3

2Installation
Receive the Unit
2-2 Medilite Microcentrifuges Thermo Scientific
Receive the Unit
All units are shipped in protective packaging.
Inspect the unit upon receipt and immediately file any damage claims with the shipper/carrier.
Prepare the Installation Site
•The unit normally resides on a bench top.
•Place the centrifuge on a clean, dry surface, to make certain that the suction feet at the bottom grip
the surface firmly. Keep the area beneath the unit free of debris and loose materials..
•A safety zone of at least 30 cm (12”)must be maintained around the centrifuge.
•The centrifuge should not be exposed to heat and strong sunlight.
•The set-up location must be well-ventilated at all times.
•Lift the lever to release the latch. Remove the soft material covering the plastic shields (6 or 12)
that are shipped in position inside the rotor.
•Press down on the center hub to ensure that the rotor is secured to the shaft. Turn rotor by hand to
verify it turns freely.
•Inspect the shields for damage. Make sure the flange of the shield is flush with the surface of the
rotor.
Verify Power Configuration
Verify that the correct power cord and connector is provided for your installation.
The unit requires a grounded power supply (3-outlet). If your facility does not have grounded power
outlets, arrange for proper grounding.
CAUTION The resting surface must be level, to ensure quiet, vibration-free operation. A
rigid and stable location is important. An improperly loaded centrifuge may vibrate or
move.
WARNING
No person or any hazardous materials Should be in the safety zone while the centrifuge
operates for the unlikely event of a disruption.
WARNING UV rays reduce the stability of plastics.
Do not subject the centrifuge, rotors and plastic accessories to direct sunlight.

2 Installation
Moving the Unit
Thermo Scientific Medilite Microcentrifuges 2-3
Moving the Unit
To move the unit to a new location:
1. Check that the new site meets the criterias described in “Prepare the Installation Site” on page 2-2
before moving the unit.
2. Before moving, unplug the centrifuge and remove all accessories and the rotor.
3. Position a flat object, such as a tongue depressor, near a suction cup at the bottom of the unit.
4. Lift up an edge of the cup, and insert the flat object far enough to break the vacuum suction seal.
5. When all four suction cups are disengaged, lift the unit from the work surface.
6. When the unit is in its new location, ensure that the suction cups adhere correctly to the work
surface.
WARNING ELECTRICAL HAZARD!
Do not remove the grounding pin from the centrifuge power cord. Do not use the bare
wired power cord to attach a power plug that does not have a grounding pin. The power
cord provided with the unit is correctly rated for the highest current demand. This power
cord should not be interchanged with cords from equipment with lower current demand.
Exchange of power cords between equipment may create a fire hazard.
WARNING Use caution when moving to avoid any injury

Thermo Scientific Medilite Microcentrifuges 3-1
3
Operation
Contents
•“Warnings and Cautions ” on page 3-2
•“Loading ” on page 3-2
•“Loading ” on page 3-2
•“Starting a Run ” on page 3-3
•“Stopping a Run ” on page 3-3

3Operation
Warnings and Cautions
3-2 Medilite Microcentrifuges Thermo Scientific
Warnings and Cautions
Chemical damage can appear as crazing, frosting, peeling or similar deterioration of the inner cavity or
exterior surface of the shields or rotor. Mechanical damage can appear as cracks, scratches or gouges on
the shield or rotor surfaces.
Operating Controls
Loading
1. Unlatch and open the cover.
2. Check that the rotor turns freely and that there are no loose objects in the chamber.
WARNING To Avoid Electric Shock: Plug the power cord into a grounded outlet.
Never remove the grounding prong from the power plug, or use any adapter which does
not complete the grounding circuit.
Always unplug the power cord before attempting to clean or service the centrifuge.
CAUTION Do not exceed maximum rated speed for each rotor/ accessory combination.
Maximum speeds can be found in “Technical Data” on page 1-3. All rotors and accessories
are stamped with their cat. no.for easy identification.
Do not spin any fluids which have a specific gravity greater than 1.5.
Do not operate the unit if the shields or rotor show signs of damage. If deterioration is
visible, replace the affected parts immediately.
Do not open the lid while the unit is spinning.
Ensure that loads are properly balanced around the rotor to minimize vibration. All
Thermo Fisher Scientific accessories are stamped with their weight for easy balancing.
Lid Opener
Timer

3 Operation
Starting a Run
Thermo Scientific Medilite Microcentrifuges 3-3
3. Load the rotor symmetrically with 2, 3, 4, or 6 tubes in the 6-place rotor (but not 1 or 5) or 2, 3,
4, 6, 8, 9, or 12 in the 12-place rotor (but not 1, 5, 7 or 11).
The tubes must be of equal size and with contents of equal (within 1 gram) weight.
4. Close the cover securely, ensuring that the latch is engaged.
Starting a Run
1. Turn the timer knob to the desired run duration.
For runs of under 5 minutes, turn the timer past the five minute setting and then back to the
desired time).
For runs in the HOLD mode (infinite duration), turn the knob speed counter-clockwise to the
∞setting.
The motor will take several minutes to reach top speed..
Stopping a Run
1. To stop a run, turn the timer knob to zero or allow the time to expire.
2. Wait until the rotor has come to a complete stop, then unlatch and open the cover.
Note Periods of slight vibration during acceleration are normal. If excessive vibration occurs, turn
the timer knob to zero (0). When the rotor has come to a stop, open the cover and check that the
load is balanced. If the rotor is properly balanced and vibration persists, ensure that the suction cup
feet are clean and adhering to the surface on which the unit was placed.

Thermo Scientific Medilite Microcentrifuges 4-1
4
Maintenance
Contents
•“Care and Cleaning ” on page 4-2