Thermo Scientific Microm HM355S User manual
Thermo Fisher Scientific Operation Manual HM335S 1
Part of Thermo Fisher Scientific
Microm HM355S
Rotary Microtome
Operation Manual – English / German
ex Ser. No 44478
Thermo Fisher Scientific
Operation Manual HM335S
2
Thermo Fisher Scientific Operation Manual HM335S 3
Microm HM355S
Rotary Microtome
Operation Manual – English
ex Ser. No 44478
Thermo Fisher Scientific
Operation Manual HM335S
04
© 2011 ermo Fisher Scientic. All rights reserved.
ermo Fisher Scientic Microm International GmbH is an ISO 9001 Company.
ermo Scientic is the trading name of ermo Fisher Scientic Microm International GmbH.
All other trademarks are the property of ermo Fisher Scientic and its subsidiaries. ermo Fisher
Scientic makes every attempt to ensure that the information contained in this support documentation is
correct and clearly stated but does not accept responsibility for any errors or omissions. e development of
ermo Scientic products and services is an ongoing process. Please ensure that any published information
you use as a reference is up to date and relates to the condition of the product. If necessary, check with ermo
Fisher Scientic or your local ermo Fisher Scientic representative.
is Operation Manual may not, in whole or in part, be copied, photocopied, reproduced, translated or con-
verted to any electronic or other form without prior written consent of ermo Fisher Scientic.
All information contained in this manual is proprietary and condential, and the exclusive property of ermo
Fisher Scientic. is manual is protected by copyright and any reproduction is prohibited. It is only be used
by the individuals to whom it has been made available through ermo Fisher Scientic.
Contact addresses Anatomical Pathology
Microm International GmbH
part of ermo Fisher Scientic
Otto-Hahn-Strasse 1a
69190 Walldorf
Germany
Tel: +49 (0) 6227 836-0
Fax: +49 (0) 6227 836-111
www.ap-walldorf.com
info.dxd.dewal@thermosher.com
4481 Campus Drive
Kalamazoo
MI 49008, USA
Tel: 1-800-522-7270
Fax: +1 269-372-2674
www.thermo.com/pathology
e ermo Scientic Microm HM355S meets the following CE Mark requirements:
In Vitro Diagnostic Directive 98/79/EC
11/11
Preface
Thermo Fisher Scientific Operation Manual HM335S 05
Dear valued customer,
ank you for buying a ermo Scientic instrument.
Before using the instrument you purchased, please read these operating
instructions carefully to familiarize yourself with the proper operation
and functions. To avoid risks to the operator and technical damage to
the instrument, it must only be used in accordance with its intended
use (page 11). Any other use of the instrument is considered improper!
All warranties and guarantees would then be null and void.
is Operation Manual was specially written for the routine user work-
ing with the Rotary Microtome HM355S. e chapters contain useful
and important information and should be considered during the daily
routine work.
Only skilled or specially trained personnel should operate the micro-
tome and perform such tasks as clamping the specimens, trimming and
rst-cuts, sectioning and transferring sections onto a slide. e safety
instructions given in this manual, the regular safety standards as well as
the regulations and hygiene measures of your respective lab should be
strictly observed.
Please keep this Operation Manual near to the instrument for later
consultation.
is Operation Manual will be supplied together with each instrument.
Additional copies can be ordered at the nearest ermo Fisher Scientic
sales oce by giving the serial number of the instrument (placed on the
rear panel) the part number of the instruction manual and the number
of version.
• English/German (printed version): 387861
• CD-Rom (including English, German, Italian, Spanish, French):
387862
Welcome
Preface
Thermo Fisher Scientific
Operation Manual HM335S
06
Contents
Important Information and Safety
Symbols 08
Personal Safety 08
General Safety 10
Intended Use 11
Introduction 12
Description Rotary Microtome Microm HM355S 12
Scope of Delivery 13
Additional Equipment (optional) 14
Technical Data Sheet 16
Operating Instructions 17
Setting up the Microtome 17
Initial Start-up 20
Using the Food Pedal (optional) 21
Operating Panel 22
Display and Key Functions 23
Setting Cutting Window 23
Starting and Stopping of the Cutting Drive 23
Hand Wheel Brakes 24
Emergency Stop 25
Cutting Process Indicators 26
Section Counter 26
Section ickness Sum 27
Remaining Distance to Front End Position 27
Setting Section and Trimming ickness 28
Trimming and First Cuts 29
Fine Feed 30
Cutting Movement and Retraction 30
Motorized Cutting Drive 32
Specimen Coarse Feed 32
Operating Modes 33
Selection of Operating Modes 33
Time and Date 36
Display Mode 36
Turning o the “Retraction” Function 37
Language Selection for the Display 38
Setting Cutting Speed 39
Factory Defaults 40
Memory Function 40
Rocking Mode Function 41
Push-in Hand Wheel Handle 42
Adapters for Specimen Clamping 43
Adapter, non-orienting 43
Adapter, orienting, Specimen Orientation 43
Chapter 1
Chapter 2
Chapter 3
Contents
Thermo Fisher Scientific Operation Manual HM335S 07
Changing and/or Fastening Specimen Clamps 44
Readjusting Specimen Clamps 45
Specimen Clamping 46
Universal Cassette Clamp 46
Universal Cassette Clamp, adjustable 46
Standard Specimen Clamp 47
Insert for Round Specimens, V-Insert and V-Distance piece 48
Foil Clamp 49
Segment Arc and Universal Specimen Holder 50
Knife and Blade Carriers 50
Disposable Blade Carrier “ER” 51
Disposable Blade Carrier “E” 53
Knife Carrier “C” 55
Re-adjusting Knife and Blade Carriers 57
Section Waste Tray with Integrated Arm Rest 58
Large Field Magnier 58
Working with the Microtome 60
Sectioning Instructions 60
How to Avoid Errors 63
Possible Sources of Errors – Cause and Removal 64
Maintenance and Care 65
Cleaning and Care 65
Maintenance 66
Replacement Work 67
Conditions for Transportation 68
Returning the Instrument for Repair or Routine Maintenance 68
Disposal of the Instrument after Final Shutdown 69
Warranty Statement and Certification 70
Chapter 4
Chapter 5
Chapter 6
Chapter 7
Contents
Thermo Fisher Scientific
Operation Manual HM335S
08
Chapter 1
Important Information
e following symbols and conventions are used throughout this
manual and on the instrument.
WARNING
or
CAUTION
S
. F -
, .
Note:
Special Instructions regarding operation of the instrument
H
azard of hand injuries
.
CAUTION
– general danger spot
.
.
Biohazard
.
Waste disposal
: -
.
.
.
.
instrunctions for using microtome knife
,
. ,
. ,
.
.
Symbols
Personal Safety
Important Informations
Symbols
Thermo Fisher Scientific Operation Manual HM335S 09
-
. T
hazard of Biological danger
S
. , -
.
, ,
:
.
. -
, -
.
, (..
, , ) .
.
sticker
“stop“
A -
-
. A
. A .
D .
.
emergency stop
.
.
.
().
. T
.
sticker
“
Brake“ With arroW
A “”
- -
. T
. H -
–
.
Important Informations
Safety
STOP
STOP
Thermo Fisher Scientific
Operation Manual HM335S
10
caution!
.
.
.
.
grounding the instrument
,
. T
.
(IEC) -
. ,
.
.
caution: high voltage
.
.
.
caution – notify a service technician!
.
.
danger in flammaBle environment
-
.
hazard of electromagnectic interference
,
.
. / ,
.
hazard of radiation
.
,
.
, -
, (..
, , ) .
.
Important Informations
Safety
General Safety
Thermo Fisher Scientific Operation Manual HM335S 11
Waste disposal
.
e ermo Scientic Rotary Microtome Microm HM355S is a uni-
versal heavy duty microtome for specially sophisticated paran, and
hard sectioning techniques in biology, medicine and industry.
Only skilled or specially trained personnel must operate the microtome,
i.e. clamping the specimens, trimming and rst cuts, sectioning and
transferring sections onto a slide. e individual safety measures as well
as the regulations and hygiene measures of your respective lab must
strictly be observed.
Note!
is operating manual is part of the product. Always keep this manual near
the instrument!
Intended Use
Important Informations
Intended Use
Thermo Fisher Scientific
Operation Manual HM335S
12
Chapter 2
Introduction
e ermo Scientic Rotary Microtome Microm HM355S is a uni-
versal heavy duty microtome for specially designed for sophisticated
paran and hard sectioning techniques in biology, medicine, industry
and research.
e HM355S sets new ergonomical standards concerning operation
and comfort. e instrument is equipped with a section waste tray with
integrated arm rest. e waste tray is built around and under the knife/
blade carrier for direct collection of section waste.
is model can be equipped with all compatible specimen clamps, knife
and blade holders (see Additional Equipment) of the Rotary Microtome
series. In addition, the stereomicroscope or the large eld magnier can
be adapted.
e HM355S will cut sections in a range from 0,5 µm up to 100 µm.
For the protection of knife and specimen, the instrument retracts the
specimen at the end of the cut. If desired, the function <retraction> can
be turned o. A trimming function with dened steps from 5 µm to
500 µm permits the ne adjustment up to the rst cuts and results in
larger section thicknesses when trimming.
e manual rotary movement of the hand wheel of the HM355S is
converted into a vertical movement of the specimen clamping system.
Sectioning is carried out by knives or blades, which must be adjusted
and xed on the knife/blade carrier.
e electronically controlled motor drive with precision tachometer
generator guarantees an extremely fast adjustment to section force varia-
tions and constant cutting speed. It also ensures optimal section quality
in each eld of application.
e motorized coarse feed system allows the continuous specimen for-
ward and backward travel with variable speed settings. is way, speci-
men and knife/blade edge distance can be adjusted quickly.
e operating panel is placed on the left side of the microtome. It can
be removed and used separately, also on the right side of the instrument.
To do this, the operating knobs can be removed and installed on the
other side of the operating panel. e touchpad keyboard is clearly ar-
ranged for easy and safe operation.
e selected section thickness, trimming thickness, section counter, sum
of section thicknesses and remaining travel to the front end
position as well as speed of the cutting movement, the operating mode
Description Rotary
Microtome HM355S
Introduction
Description HM355S
Thermo Fisher Scientific Operation Manual HM335S 13
and the current date and time are indicated on the display of the operat-
ing panel.
e number of the sections made can be shown on the section counter
on the display. After each downward movement of the specimen holder,
the number on the section counter increases by 1. e sum of the sections
carried out can also be seen on the display. Trimming values and sec-
tioning values are added up. Section counter and sum of section thick-
nesses can be reset to zero at any time by using of the RESET-button.
e remaining travel to the front end position can also be shown on the
display. e remaining travel, which is still available for sectioning, is
shown in microns. If the specimen holder is in the back end position,
the display shows 28 000 µm. is number decreases, the further the
specimen holder is moved towards the front.
e knife/blade carriers are designed so that the knives/blades can be
easily clamped in place and adjusted. e microtome is perfectly
balanced for the use with the universal cassette clamp (715020). Using
other clamps can result in slight object movements when the hand
wheel brake is not activated.
For the users safety, the instrument is equipped with an emergency stop
device. An electronic and a mechanical hand wheel brake and a hand
wheel handle, which can be pushed in, are installed for further safety.
e fast freezing unit KS-34 allows frozen sectioning with the specimen
temperature as low as - 45° C.
e Rotary Microtome Microm HM355S is supplied with the follow-
ing accessories:
Quantity Description
1 Operating Panel
1 Section waste tray, big
1 Cover plate, brushed aluminium
1 Cover
1 Brush
1 Paraffin repellent PARA GARD, 100 ml
1 Operation Manual, printed
1 CD-Rom with Operation Manual
1 Power Cord (230V)
1 Power Cord (115V)
1 Power Cord UK (optional, for deliveries to the UK)
Scope of Delivery
Standard Equipment
Introduction
Scope of Delivery
Thermo Fisher Scientific
Operation Manual HM335S
14
Additional available equipment (optional)
Description Cat.-No.
Section Transfer System STS
Section Transfer System STS with Blade Holder TE 771200
Standard Specimen Clamp for STS 715550
Transfer Surface for STS 575170
Cool-Cut
with Universal Cassette Clamp 771110
with Standard Specimen Clamp 771120
Fast Freezing Unit KS-34
100…115V/50…60Hz 770210
220…240V/50…60Hz 770220
Foot Pedal with Interlock Connector 640380
Knife and Blade Carrier
Disposable Blade Carrier ER 705830
Disposable Blade Carrier E 705800
Knife Carrier C 705810
Standard Knife Carrier N 705820
Specimen Clamps
Standard Specimen Clamp 715010
Universal Cassette Clamp 715020
Adjustable Universal Cassette Clamp 716130
Adjustable Universal Cassette Clamp, for Macro-Cassettes 716120
Adjustable Universal Cassette Clamp, for MacrOflow-Cassettes 716150
Foil Clamp 715030
Sandwich Supporting Material 176010
Insert for Round Specimens, Ø 6 mm 715070
Insert for Round Specimens, Ø 15 mm 715080
Insert for Round Specimens, Ø 19 mm 715280
Insert for Round Specimens, Ø 25 mm 715090
V-Insert 715100
V-Distance Piece 715320
Segment Arc 715590
Universal Specimen Holder 715060
Additional Equipment
Introduction
Additional Equipment
Thermo Fisher Scientific Operation Manual HM335S 15
Description Cat.-No.
Optical Accessories
Large Field Magnifier, 220 V 760160
Large Field Magnifier, 120 V 760170
Zoom-Stereomicroscope Zeiss Stemi 2000 755210
Adapter for Stemi 2000 532090
Ring Illumination, 100….240V/50…60Hz 760340
Disposable Blades
SEC 35 152200
SEC 35e 152215
SEC 35p 152570
Microtome Knives
Steel Knives, Type C
12 cm 152010
16 cm 152020
18,5 cm 152270
22 cm 152030
Steel Knives, Type D
12 cm 152060
16 cm 152070
22 cm 152080
Tungsten carbide Knife, Type D
16 cm 152120
Knife Cases, Always for 2 Knifes
12 cm 152220
16 cm 152230
18,5 cm 152280
22 cm 152240
Paraffin Repellent, PARA GARD, 100 ml 350170
Lubrication Oil, 100 ml 350110
Lubrication Oil, 250 ml 350120
Introduction
Additional Equipment
Thermo Fisher Scientific
Operation Manual HM335S
16
Microtome Thermo Scientific Microm HM355S
Feed Section Thickness
Range
0,5 – 100 µm
Resolution 0,5 µm from 0,5 – 5 µm
1 µm from 5 – 20 µm
2 µm from 20 – 30 µm
5 µm from 30 – 60 µm
10 µm from 60 – 100 µm
Trimming Thickness Range 5 – 500 µm
Resolution 5 µm from 5 – 30 µm
10 µm from 30 – 100 µm
20 µm from 100 – 200 µm
50 µm from 200 – 500 µm
Specimen Retraction
during Return Travel
40 µm, disengageable
Horizontal Feed Range 28.000 µm
Vertical Specimen Stroke 72 mm
Section Counter 5-digit, with reset
Section Thickness Sum 5-digit, with reset
Remaining Travel
to Front End Position
5-digit
Specimen Size when using a Standard
Specimen Clamp
max. 55 x 50 mm
Specimen Size when using a Macro-Speci-
men Clamp
max. 68 x 50 mm
Specimen Orientation X- and Y-axes universal 8°
Samples Rotation 360°
Cutting Drive motorized, electronically
controlled
Modes of Operation interval, single, multi and
continuous mode
Cutting Speed 0 – 450 mm/s
Coarse Feed motorized, variable speed
Storage Temperature
Range
- 20° C up to + 50° C
Operating Conditions for indoor use only + 10° C up to + 40° C
(at a max. rel. humidity of 60 %)
altitude up to 2000 M.S.L.
Floor Loading
Requirements
110 kg/m2
Power Requirements 100...240 V 1,6 A +/-10%
50...60 Hz
Technical Data Sheet
Introduction
Technical Data Sheet
Thermo Fisher Scientific Operation Manual HM335S 17
Microtome Thermo Scientific Microm HM355S
Pollution Degree 2
Overvoltage Category II
Sound Pressure 42 dB(A)
Dimensions 410 mm x 520 mm x 280 mm
(wide/deep/high)
Weight 31 kg
Chapter 3
Operating Instructions
Cut through the bands around the carton.
• Open the carton.
• Remove the accessories.
• On the lower front and rear side of the instrument, there are two
recessed grip to lift and carry the microtome.
Note!
Do not use the hand wheel handles to lift or carry the instrument.
• Lift the microtome out of the carton.
• Choose a place for the microtome where the mains switch can be
reached at all times.
• Place the microtome on a stable and vibration free table, as sectioning
can be inuenced by nearby instruments which generate vibrations.
• Hand wheel must be free and accessible in a comfortable way.
• Remove the separately packed section waste tray and install it at the
base plate from the front side.
• Remove the separately packed cover plate and place it on the top of
the microtome.
• In the rear part at the bottom of the instrument, there are sliding
feed for an easy moving of the microtome.
• Slightly lift the base at the front end only and slide the microtome
into place.
Note!
Remove the section waste tray to move or carry the instrument. e section
waste tray can be pulled out of its proper position.
Setting up the
Microtome
Operating Instructions
Setting up the Microtome
Thermo Fisher Scientific
Operation Manual HM335S
18
Frontal View
Rear View
Operating Panel
(page 22)
Push-In Hand
Wheel Handle
(page 42)
Push-In Hand Wheel
Handle (page 42)
Specimen Orientation
(page 43 ff)
Emergency Stop
Button (page 25)
Connectors
(page 20)
Fuses – Power Switch –
Power Cord (page 20)
Operating Instructions
View
Thermo Fisher Scientific Operation Manual HM335S 19
Lateral View Left Side
Lateral View Right Side
Operating Panel
(page 22)
Push-In Hand Wheel
Handle (page 42)
Specimen Orientation
(page 43 ff)
Operting Instructions
View
Mechanical Hand
Wheel Brake (page 24)
Specimen Orientation
(page 43 ff)
Thermo Fisher Scientific
Operation Manual HM335S
20
Note!
A safe function of the Microtome is only ensured, if the equipment possesses
a temperature within the specied operating conditions (see technical data
sheet, page 14).We highly recommend that the Microtome rests at least 2
hours after unpacking at ambient temperature before switching it on for the
rst time.
Note!
Before starting section, instrument, knife carrier and section waste tray
should be treated with the included or any other commercially available
paran repellent. is medium considerably reduces the adhesive property
of paran sections to the individual parts (see page 13 and 15, standard
and optional accessories)
Note!
e type of examination materials used and all special conditions for their
processing, pre-treatment and, if necessary, storage as well as instrument
controls for correct and safe operation are the responsibility of the operator.
e operator is also responsible for special equipment and materials and/or
reagents used for the operation of the instrument.
e operating panel, which is packed separately, can be attached to the
instrument or used freestanding. First connect the operating panel with
the instrument:
Initial Start-up
[a] [d]
[b] [e]
[c]
[f]
Operating Instructions
Initial Start-up
a For Service Purposes only
b Operating Panel
c Foot Pedal
d Cover For Fuses
e Power Switch
f Power Socket
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