Thermo Scientific Finnpipette F3 User manual

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Finnpipette®F3
Single Channel
Variable & Fixed Volume
Instructions for Use
Bedienungsanleitung
Guide d´utilisation
Instrucciones de uso
取扱説明書
User Manual

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This product complies with the European Union Directive 98/79/EC, and it is marked with
a CE-marking.
When the product is used in applications related to the directive 98/79/EC, read the
additional information at www.thermofisher.com or contact the manufacturer at info.
pipettes@thermofisher.com to ensure correct and safe use.
The CE-mark covers the system containing the CE-marked Finnpipette F3 pipette and the
CE-marked Finntips.
Product specifications are subject to change without prior notice. Finnpipette®and Finntip®
are registered trademarks of Thermo Fisher Scientific Oy.
Ce produit est conforme à la directive de l’Union européenne 98/79/CE et porte le
marquage CE.
Lorsque le produit est utilisé pour des applications relatives à la directive 98/79/EC, veuillez
prendre connaissance des informations complémentaires à www.thermofisher.com ou
contacter le constructeur à info.pipettes@thermofisher.com. Ceci pour garantir un usage
adéquat et sûr.
Le marquage CE s’applique au système comprenant la Finnpipette F3 et les cônes Finntip.
Les spécifications du produit sont sujettes à modification sans avis préalable. Finnpipette®
et Finntip®sont des marques déposées de Thermo Fisher Scientific Oy.
Este producto cumple la Directiva de la Unión Europea 98/79/CE y presenta el marcado CE.
Cuando el producto sea usado en aplicaciones afectadas por la directiva 98/79/EC, por favor,
lea la información adicional en www.thermofisher.com o contacte con el fabricante en
info.pipettes@thermofisher.com para asegurar su uso correcto y seguro.
El marcado CE corresponde al sistema que contiene la pipeta Finnpipette F3 con el marcado
CE y las puntas de pipeta Finntip con marcado CE.
Las especificaciones del producto pueden cambiar sin previo aviso. Finnpipette®y Finntip®
son marcas registradas de Thermo Fisher Scientific Oy.
本製品は欧州指令98/79/ECに準拠しており、CEマーキングが付いています。
製品を指令98/79/ECに関連するアプリケーションで使用するときは、適切で安全に
ご使用いただくため、 www.thermofisher.com に掲載の追加情報をお読みになる
か、製造元 [e-mail] にお問い合わせください。
CEマークは、CEマークの付いた Finnpipette F3ピペットおよびCEマークの付いた
Finntipピペットチップを含むシステムを対象としています。
製品仕様は事前の予告なく変更されることがあります。フィンピペット®とフィンチ
ップ®は、サーモフィッシャーサイエンティフィックの登録商標です。
Dieses Produkt entspricht der Europäischen Richtlinie 98/79/EG und ist mit dem CE-Symbol
gekennzeichnet.
Sollte das Produkt in Anwendungen verwendet werden, die in Verbindung zur Direktive
98/79/EC stehen, lesen Sie die Zusatzinformationen auf www.thermofisher.com oder
kontaktieren Sie den Hersteller unter info.pipettes@thermofisher.com, um die richtige
und sichere Verwendung zu gewährleisten.
Die CE-Kennzeichnung bezieht sich auf die Kombination der Finnpipette F3 mit Finntip-
Pipettenspitzen.
Wir behalten uns das Recht auf unangekündigte Änderungen der Produktspezifikationen vor.
Finnpipette®und Finntip®sind eingetragene Warenzeichen der Fa. Thermo Fisher Scientific Oy.

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English
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日
本
語
CONTENTS
PRODUCT DESCRIPTION 4
PACKAGE 4
SAFETY 5
PIPETTE OPERATION 6
PIPETTING TECHNIQUES 6
CALIBRATION AND ADJUSTMENT 7
MAINTENANCE 10
TROUBLE SHOOTING 12
SPARE PARTS 53-56
INHALT
PRODUKTBESCHREIBUNG 13
PACKUNGSINHALT 14
SICHERHEIT 14
PIPETTENFUNKTION 15
PIPETTIERMETHODEN 15
KALIBRIERUNG UND JUSTIERUNG 17
WARTUNG 20
FEHLERBEHEBUNG 22
ERSATZTEILE UND ZUBEHÖR 53-56
SOMMAIRE
DESCRIPTION DU PRODUIT 23
CONDITIONNEMENT 23
CONSIGNES DE SéCURITé 24
UTILISATION DE LA PIPETTE 25
MéTHODES DE PIPETAGE 25
CALIBRAGE 26
ENTRETIEN 29
EN CAS DE PROBLEME 31
PIèCES DéTACHéES 53-56
CONTENIDO
DESCRIPCIÓN DEL PRODUCTO 32
PAQUETE 33
SEGURIDAD 33
USO DE LA PIPETA 34
TéCNICAS DE PIPETEO 34
CALIBRACIÓN Y AJUSTE 36
MANTENIMIENTO 39
SOLUCIÓN DE PROBLEMAS 41
PIEZAS DE RECAMBIO 53-56
目次
製品について 42
パッケージ 42
安全にお使いいただくために 43
ピペットの操作 44
ピペッティングテクニック 44
キャリブレーション 45
メンテナンス 48
トラブルシューティング 49
保証規定 51
パーツ及び付属品 53-56

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Product description
The Finnpipette F3 is a continuously adjustable, general purpose micropipette for sampling and
dispensing accurate liquid volumes.
It operates on an air displacement principle (i.e. an air interface) and uses detachable, disposable tips.
The adjusted delivery volume is displayed digitally on a readout window in the handle.
The twelve different models of Finnpipette F3 pipettes cover a volume range from 0.2 µl to 10 ml.
Order No. Volume Range
Color code Finntip
4640110 0,2 µl to 2 µl pink Flex 10, 10, 20, 50
4640000 1 µl to 10 µl pink Flex 10, 10, 20, 50
4640010 1 µl to 10 µl yellow
Flex 200, 250 Univ., 200 Ext, 300, Flex 300
4640020 2 µl to 20 µl turquoise 50
4640030 2 µl to 20 µl yellow Flex 200, 250 Univ., 200 Ext, 300, Flex 300
4640080 5 µl to 50 µl turquoise 50
4640090 5 µl to 50 µl yellow Flex 200, 250 Univ., 200 Ext, 300, Flex 300
4640040 10 µl to 100 µl yellow Flex 200, 250 Univ., 200 Ext, 300, Flex 300
4640050 20 µl to 200 µl yellow Flex 200, 250 Univ., 200 Ext, 300, Flex 300
4640060 100 µl to 1000 µl blue Flex 1000, 1000, 1000 Ext
4640100 0,5 ml to 5 ml green 5 ml
4640070 1 ml to 10 ml red 10 ml, Flex 10 ml Ext
The eleven different models of Finnpipette F3 Fixed Volume pipettes cover a volume range from
1 µl to 10 ml.
Order No. Volume Range Color code Finntip
4650000 1 µl dark blue Flex 10, 10, 20, 50
4650010 5 µl dark blue Flex 10, 10, 20, 50
4650020 10 µl dark blue Flex 200, 250 Univ., 200 Ext, 300, Flex 300
4650090 20 µl dark blue Flex 200, 250 Univ., 200 Ext, 300, Flex 300
4650030 25 µl dark blue Flex 200, 250 Univ., 200 Ext, 300, Flex 300
4650040 50 µl dark blue Flex 200, 250 Univ., 200 Ext, 300, Flex 300
4650050 100 µl dark blue Flex 200, 250 Univ., 200 Ext, 300, Flex 300
4650100 200 µl dark blue Flex 200, 250 Univ., 200 Ext, 300, Flex 300
4650060 250 µl dark blue Flex 1000, 1000, 1000 Ext
4650070 500 µl dark blue Flex 1000, 1000, 1000 Ext
4650080 1000 µl dark blue Flex 1000, 1000, 1000 Ext
Digital display
The adjusted delivery volume is clearly indicated in the
large digital display on the handle.
Raw materials
The Finnpipette F3 is made of mechanically durable and chemically resistant materials.
The tip cone modules can be repeatedly autoclaved at 121°C.
Description of tips
Finntips are recommended for use with the Finnpipette F3.
They are made of virgin natural colour polypropylene, generally regarded as the only
contamination free material suitable for tips. Finntips are also autoclavable (121°C).
Package
The Finnpipette F3 is shipped in a specially designed package containing the following items:
1. The Finnpipette
2. Service tool
3. Bag of grease 1g (Order No. 3300200)
4. Instructions for Use
5. Calibration certificate / Warranty certificate

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Safety
Cautions are marked with this symbol .
Intended use
The intended use of the device is to transfer liquids in the volume range of 0,2 µl to 10 ml.
The F3 Finnpipette pipette and Finntips are designed as a component of an analyzing system
for an end user, who is responsible for validating the system to ensure reliable and safe results.
Damage to health
Follow general procedures for hazard prevention and safety instructions; e.g. wear
protective clothing, eye protection and gloves.
For use and waste disposal of hazardous (e.g. radioactive and potentially infectious)
material, follow the safety instructions and general laboratory practice.
The pipette and tips are not intended for in vivo use. Do not use the pipette for pipetting any
liquid to be injected into a human body.
Do not eject the tip towards anybody.
The Finnpipette F3 is to be used by trained personnel with required laboratory skills. The
instructions for use must be read prior to and during the use of the device (pipette and tip). The
F3 pipette can be used between +4°C and +40°C.
Incorrect dispensing results
Performance may vary due to:
a. pipetting method (forward pipetting technique recommended)
b. temperature (air, liquid, vessel, pipette, and tip)
c. pressure
d. humidity
e. operator, e.g. thumb movement, pipetting angle
f. liquid density, viscosity and vapor pressure
g. type of tip
If the pipetting performance is critical to the outcome of a specific application, the result has
to be assured with an alternative test, and if this is not an option, by duplicate testing.
The possibility of an incorrect volume delivery during pipetting cannot be entirely mitigated.
To avoid inaccurate dispensing and/or leakage, check that the tip is properly attached to
the pipette.
When rotating the volume adjustment button, do not exceed the volume range of the pipette.
Otherwise the pipette may be damaged or affect pipetting performance.
Pipetting performance cannot be guaranteed if the tip is reused.
Choose only a tip and pipette with matching color coding.
Disposal of the Materials
Follow laboratory and country-specifi c procedures for the disposal of biohazardous or radioactive
waste. Refer to local regulations for the disposal of infectious material.
Warning The tips can be potentially infectious. Dispose of all used disposable tips as
biohazardous waste.
Warning Decontaminate the pipette before disposal.
Waste disposal according to local instructions taking into account legislation in place. For more
information, please contact us at info.pipettes@thermofisher.com or your local Thermo
Fisher Scientific representative.
Regarding the original packaging and packing materials, use the recycling operators known
to you.

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Pipette operation
Setting the delivery volume
1. Set the delivery volume using the push button on the top of
the pipette. To increase the delivery volume, turn the push button
counterclockwise. To decrease the delivery volume, turn it clockwise.
2. Make sure that the desired delivery volume clicks into place.
3. Do not set volumes outside the pipette´s specified volume range.
Using excessive force to turn the push button outside the range
may jam the mechanism and eventually damage the pipette.
Safety Label
You can mark the pipette application, your initials, the
calibration date, etc. on the safety label. Remove the old
label with a sharp needle. Mark the new label with a pencil
and slide the label back in place.
Tip ejection
To help eliminate the risk of contamination, each pipette
is fitted with a tip ejector system. To release the tip, point
the pipette at suitable waste receptacle and press the tip
ejector with your thumb.
Pipetting techniques
Push and release the push button slowly at all times particularly when working with
high viscosity liquids. Never allow the push button to snap back.
Make sure that the tip is firmly attached to the tip cone. Check for foreign
particles in the tip.
Before you begin your actual pipetting work, fill and empty the tip 2-3 times with
the solution that you will be pipetting. Hold the pipette in an upright position while
aspirating liquid. The grippy should rest on your index finger. Make sure that the tips,
pipette and solution are at the same temperature.
Forward technique
Fill a clean reagent reservoir with the liquid to be dispensed.
1 Depress the push button to the first stop.
2. Dip the tip under the surface of the liquid in the reservoir to a depth of about 1 cm and
slowly release the push button. Withdraw the
tip from the liquid touching it against the edge of
the reservoir to remove excess liquid.
3. Deliver the liquid by gently depressing the push
button to the first stop. After a delay of about
one second, continue to depress the push button
all the way to the second stop. This action will
empty the tip.
4. Release the push button to the ready position. If
necessary, change the tip and continue pipetting.
Reverse technique
The reverse technique is suitable for dispensing liquids that have a high viscosity or a tendency
to foam easily. The technique is also recommended for dispensing very small volumes. Fill a
clean reagent reservoir with the liquid to be dispensed.
1. Depress the push button all the way to the
second stop.
2. Dip the tip under the surface of the liquid in the
reservoir to a depth of about 1 cm, and slowly
release the push button. This action will fill the
tip. Withdraw the tip from the liquid touching
it against the edge of the reservoir to remove
excess liquid.
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3. Deliver the preset volume by gently depressing the push button to the first stop. Hold the
push button at the first stop. Some liquid will remain in the tip and this should not be included
in the delivery.
4. The remaining liquid should either be discarded with the tip or pipetted back into the
container.
Repetitive technique
The repetitive technique offers a rapid and simple procedure for repeated delivery of the same
volume. Fill a clean reagent reservoir with the liquid to be dispensed.
1. Depress the push button all the way to the second stop.
2. Dip the tip under the surface of the liquid in the
reservoir to a depth of about 1 cm, and slowly re
lease the push button. This action will fill the tip.
Withdraw the tipfrom the liquid touching against
the edge of the reservoir to remove excess liquid.
3. Deliver the preset volume by gently depressing
the push button to the first stop. Hold the push
button at the first stop. Some liquid will remain in
the tip and this should not be included in the delivery.
4. Continue pipetting by repeating steps 3 and 4.
Pipetting of heterogeneous samples
(deproteinization in blood glucose determination, for example)
Use steps 1 and 2 of the forward technique to fill the tip with blood.
Wipe the tip carefully with a dry clean tissue.
1. Immerse the tip into the reagent and depress the
push button to the first stop, making sure the tip
is well below the surface.
2. Release the push button slowly to the ready position.
This will fill the tip. Keep the tip in the solution.
3. Depress the push button to the first stop and
release slowly. Keep repeating this procedure
until the interior wall of the tip is clear.
4. Finally, depress the push button all the way to the
second stop to completely empty the tip.
Calibration and adjustment
All Finnpipettes are factory calibrated and adjusted to give the volumes as specified with
distilled or deionized water using the forward pipetting technique. It should be noted that the
use of other pipetting techniques may affect the calibration results. The pipettes are constructed
to permit re-adjustment for other pipetting techniques or liquids of different temperature and
viscosity.
Device requirements and test conditions
An analytical balance must be used. The scale graduation value of the balance should be chosen
according to the selected test volume of the pipette:
Volume range readable graduation
under 10 µl 0.001 mg
10-100 µl 0.01 mg
above 100 µl 0.1 mg
Test liquid: Water, distilled or deionized, “grade 3” water conforming ISO 3696. Tests are done
in a draft-free room at a constant (±0.5°C) temperature of water, pipette and air between 15°C
to 30°C. The relative humidity must be above 50%. Especially with volumes under 50 µl the
air humidity should be as high as possible to reduce the effect of evaporation loss. Special
accessories, such as the evaporation trap, are recommended.
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Procedure to check calibration
The pipette is checked with the maximum volume (nominal volume) and with the minimum
volume. A new tip is first pre-wetted 3-5 times and a series of ten pipettings is done with
both volumes. A pipette is always adjusted for delivery (Ex) of the selected volume. Use of
forward pipetting technique is recommended. The maximum permissible errors are designed for
forward method. It is recommended to calibrate all channels of the multichannel pipette, but a
suitable method is also to calibrate only the edge channels. Adjustment check prior calibration is
done using one of the middle channels. It is recommended to use the ISO8655 calibration limits
presented in the following tables or user-defined specifications.
Procedure:
1. Do 10 pipettings with the minimum volume.
2. Do 10 pipettings with the maximum volume.
3. Calculate the inaccuracy (A) and imprecision (cv) of both series.
4. Compare the results to the limits.
If the calculated results are within the selected limits, the adjustment of the pipette is correct.
ISO8655 calibration specifications
Thermo Scientific Finnpipette F3 specifications according to ISO8655 standard.
Finnpipette F3 Variable Volume Single Channel
Range Volume Inaccuracy Imprecision
µl µl % s.d. µl cv%
0.2-2 µl 2 ±0.080 ±4 0.040 2.0
0.2 ±0.080 ±40 0.040 20.0
1-10 µl 10 ±0.120 ±1.2 0.080 0.8
1 ±0.120 ±12 0.080 8.0
2-20 µl 20 ±0.20 ±1.0 0.10 0.5
2 ±0.20 ±10.0 0.10 5.0
5-50 µl 50 ±0.50 ±1.0 0.20 0.4
5 ±0.50 ±10 0.20 4.0
10-100 µl 100 ±0.80 ±0.8 0.30 0.3
10 ±0.80 ±8.0 0.30 3.0
20-200 µl 200 ±1.60 ±0.8 0.60 0.3
20 ±1.60 ±8.0 0.60 3.0
100-1000 µl 1000 ±8.0 ±0.8 3.0 0.3
100 ±8.0 ±8.0 3.0 3.0
0.5-5 ml 5000 ±40.0 ±0.8 15.0 0.3
500 ±40.0 ±8.0 15.0 3.0
1-10 ml 10000 ±60.0 ±0.6 30.0 0.3
1000 ±60.0 ±6.0 30.0 3.0
Finnpipette F3 Fixed Volume Single Channel
Fixed Volume Inaccuracy Imprecision
µl µl % s.d.µl cv%
1 ±0.050 ±5.00 0.050 5.00
5 ±0.125 ±2.50 0.075 1.50
10 ±0.120 ±1.20 0.080 0.80
20 ±0.20 ±1.00 0.10 0.50
25 ±0.50 ±2.00 0.20 0.80
50 ±0.50 ±1.00 0.20 0.40
100 ±0.80 ±0.80 0.30 0.30
200 ±1.6 ±0.80 0.60 0.30
250 ±4.0 ±1.60 1.50 0.60
500 ±4.0 ±0.80 1.50 0.30
1000 ±8.0 ±0.80 3.00 0.30

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Manufacturer specification limits
Finnpipette F3 Variable Volume Single Channel
Range Volume Inaccuracy Imprecision Factory
µl µl % s.d. µl cv% calibration tip
0.2-2 µl 2 ±0,050 ±2,50 0,040 2,00 Flex 10
0,2 ±0,024 ±12,00 0,020 10,00
1-10 µl, micro 10 ±0,100 ±1,00 0,050 0,50 Flex 10
1 ±0,025 ±2,50 0,020 2,00
1-10 µl 10 ±0,100 ±1,00 0,080 0,80 Flex 200
1 ±0,035 ±3,50 0,030 3,00
2-20 µl, micro 20 ±0,20 ±1,00 0,08 0,40 50
2 ±0,06 ±3,00 0,05 2,50
2-20 µl 20 ±0,20 ±1,00 0,08 0,40 Flex 200
2 ±0,06 ±3,00 0,05 2,50
5-50 µl, micro 50 ±0,30 ±0,60 0,15 0,30 50
5 ±0,15 ±3,00 0,125 2,50
5-50 µl 50 ±0,30 ±0,60 0,15 0,30 Flex 200
5 ±0,15 ±3,00 0,125 2,50
10-100 µl 100 ±0,80 ±0,80 0,20 0,20 Flex 200
10 ±0,30 ±3,00 0,10 1,00
20-200 µl 200 ±1,2 ±0,60 0,4 0,20 Flex 200
20 ±0,36 ±1,80 0,14 0,70
100-1000 µl 1000 ±6,0 ±0,60 2,0 0,20 Flex 1000
100 ±1,0 ±1,00 0,6 0,60
0,5-5 ml 5000 ±25,0 ±0,50 10,0 0,20 5 ml
500 ±10,0 ±2,00 4,0 0,80
1-10 ml 10000 ±50,0 ±0,50 20,0 0,20 10 ml
1000 ±20,0 ±2,00 8,0 0,80
Finnpipette F3 Fixed Volume Single Channel
Fixed Volume Inaccuracy Imprecision Factory
µl µl % s.d. µl cv% calibration tip
1 ±0,040 ±4,00 0,040 4,00 Flex 10
5 ±0,070 ±1,40 0,070 1,40 Flex 10
10 ±0,090 ±0,90 0,080 0,80 Flex 200
20 ±0,14 ±0,70 0,10 0,50 Flex 200
25 ±0,15 ±0,60 0,125 0,50 Flex 200
50 ±0,30 ±0,60 0,20 0,40 Flex 200
100 ±0,40 ±0,40 0,30 0,30 Flex 200
200 ±0,80 ±0,40 0,60 0,30 Flex 200
250 ±1,0 ±0,40 0,75 0,30 Flex 1000
500 ±1,5 ±0,30 1,5 0,30 Flex 1000
1000 ±3,0 ±0,30 3,0 0,30 Flex 1000
Adjustment
Adjustment is done only for one volume. The recommended adjustment volume is the minimum
volume or 10% of the maximum volume.
1. Place the service tool into the openings of the
calibration nut at the top of the handle.
2. Turn the service tool clockwise to increase, or
counterclockwise to decrease the volume.
3. After adjustment check the calibration according
to the instructions above.
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Formulas for calculating results
Conversion of mass to volume
V = (w + e) x Z V = volume (µl)
w = weight (mg)
e = evaporation loss (mg)
Z = conversion factor for µl/mg conversion
Evaporation loss can be significant with low volumes. To determine mass loss, dispense water
to the weighing vessel, note the reading and start a stopwatch. See how much the reading
decreases during 30 seconds (e.g. 6 mg = 0.2 mg/s).
Compare this to the pipetting time from taring to reading. Typically pipetting time might be 10
seconds and the mass loss is 2 mg (10 s x 0.2 mg/s) in this example. If an evaporation trap or lid
on the vessel is used the correction of evaporation is usually unnecessary.
The factor Z is for converting the weight of the water to volume at test temperature and pressure.
A typical value is 1.0032 µl/mg at 22°C and 95 kPa. See the conversion table on page 52.
Inaccuracy (systematic error)
Inaccuracy is the difference between the dispensed volume and the selected volume of a pipette.
A = V - V0A = inaccuracy
V = mean volume
V0= nominal volume
Inaccuracy can be expressed as a relative value: A% = 100% x A / V0
Imprecision (random error)
Imprecision refers to the repeatability of the pipettings. It is expressed as standard deviation (s)
or coefficient of variation (cv)
s = standards deviation
v = mean volume
n = number of measurements
Standard deviation can be expressed as a relative value (CV) CV = 100% x S / V
Maintenance
When the Finnpipette F3 is not in use, make sure it is stored in an upright position. We
recommend a Finnpipette stand for this purpose.
The part # refer to exploded views beginning at page 53.
Short-term service
The pipette should be checked at the beginning of each day for dust and dirt on the outside
surfaces of the pipette.
Particular attention should be paid to the tip cone. No other solvents except
70 % ethanol should be used to clean the pipette.
Long-term maintenance
Annual pipette usage varies significantly among laboratories, and the
types of substances being aspirated and dispensed also vary tremendously
among laboratories. In order to maintain proper care of the pipette, it is the
laboratory’s responsibility to develop a proper maintenance program that
meets its specific needs and requirements.
The servicing procedure starts with the disassembly of the pipette.
1-1000 µl pipettes
1. Press the tip ejector.
2. Rotate the tip ejector 11 counterclockwise and pull it out.
3. Turn out the tip cone counterclockwise with the service tool.
4. Pull out the piston and other parts. Push out with piston
the rest of the piston assembly. Then turn the tip cone
upside down and tap all parts from tip cone. Remember
keep all parts in order on table for reassembly.
5. Clean the piston, the piston spring and the O-rings with a dry
napless cloth.
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