Thermo Scientific TSE Series Operating instructions

Thermo Scientific
TSE Series -86C Ultra Low Temperature Freezer
Operating and Maintenance Manual 7020933 Rev. 2

Thermo Scientific
Preface
TSE Series i
Number (Model) Capacity -
liters (cubic feet)
Voltage
/Hz
TSE240V (933) 368 (13) 230/50
TSE240D (947) 368 (13) 230/60
TSE240A (930) 368 (13) 120/60
TSE320A (931) 490 (17) 120/60
TSE320D (948) 490 (17) 230/60
TSE320V (935) 490 (17) 230/50
TSE400V (936) 652 (23) 230/50
TSE400D (949) 652 (23) 230/60
TSE400A (932) 652 (23) 120/60
TSE600D (937) 793 (28) 230/60
TSE600V (950) 793 (28) 230/50
Part No. Description Qty
34040 Key Ring 1
122005 Key 2
380520 Neoprene Cap 2
510016 1/4-20 x 5-1/2” Bolt 2
195763 Retaining Clip 1
370563 Remote Alarm Connector 1
Packing ListModels Covered
MANUAL NUMBER 7020933
2 FR-2361 5/10/12 Run cap chg - 230V elec schematic, start components expl drawing ccs
1 FR-2267 2/10/12 Updated refrigeration schematics ccs
0 FR-2267 1/4/12 Compressor change - Release 7 ccs
REV ECR/ECN DATE DESCRIPTION By
Important installer and user information:
A redundant temperature sensing device has been included in this ULT freezer.
This device is a type “T” thermocouple. For convenient access, the thermocou-
ple (Figure 1-3) terminates in an interconnect jack (Figure 1-5) behind the base
front cover. (May be located differently in chests. See Section 1.) It is strongly
recommended that this thermocouple be attached to a redundant 24 hour 7
day monitoring system with alarm capabilities. Connecting the sensor to a
monitoring and alarm system separate from the freezer provides the utmost in
product safety, should the integral system fail. s

Thermo Scientificii TSE Series
PrefacePreface
Important Read this instruction manual. Failure to read, understand and follow the instructions in this manual
may result in damage to the unit, injury to operating personnel, and poor equipment performance. s
Caution All internal adjustments and maintenance must be performed by qualified service personnel. s
Material in this manual is for information purposes only. The contents and the product it describes are subject
to change without notice. Thermo Fisher Scientific makes no representations or warranties with respect to this
manual. In no event shall Thermo be held liable for any damages, direct or incidental, arising out of or related to
the use of this manual.
©2012 Thermo Fisher Scientific. All rights reserved.
Contains Parts and Assemblies
Susceptible to Damage by
Electrostatic Discharge (ESD)
CAUTION

Thermo Scientific TSE Series iii
Preface
Important operating and/or maintenance instructions. Read the accompanying text carefully.
Potential electrical hazards. Only qualified persons should perform procedures associated with this
symbol.
Extreme temperature hazards, hot or cold. Use special handling equipment or wear special, protective
clothing.
Hot surface(s) present which may cause burns to unprotected skin, or to materials which may be
damaged by elevated temperatures.
Marking of electrical and electronic equipment, which applies to electrical and electronic equipment
falling under the Directive 2002/96/EC (WEEE) and the equipment that has been put on the market
after 13 August 2005.
This product is required to comply with the European Union’s Waste Electrical & Electronic
Equipment (WEEE) Directive 2002/96/EC. It is marked with the WEEE symbol. Thermo Fisher
Scientific has contracted with one or more recycling/disposal companies in each EU Member State
European Country, and this product should be disposed of or recycled through them. Further
information on Thermo’s compliance with this directive, the recyclers in your country and
information on Thermo products will be available at www.thermofisher.com.
4Always use the proper protective equipment (clothing, gloves, goggles, etc.)
4Always dissipate extreme cold or heat and wear protective clothing.
4Always follow good hygiene practices.
4Each individual is responsible for his or her own safety.

Thermo Scientificiv TSE Series
Preface
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TSE Series vThermo Scientific
Table of Contents
Installation and Start-up . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .1-1
Control Panel Keys, Displays, Indicators . . . . . . . . . . . . . . . . . . . . . .1-4
Operation of the Keypad . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .1-5
Choose Location . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .1-5
Install Freezer . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .1-5
Install Wall Bumpers . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .1-6
Remote Alarm Contacts . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .1-6
Install Shelves . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .1-6
Connect Unit to Electrical Power . . . . . . . . . . . . . . . . . . . . . . . . . .1-7
Attach Power Cord . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .1-7
Set Operating Temperature . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .1-8
Freezer Start-Up . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .1-8
Set Low Temperature Alarm . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .1-9
Set High Temperature Alarm . . . . . . . . . . . . . . . . . . . . . . . . . . . . .1-9
Run Mode . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .1-10
Calibrate . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .2-1
Calibrate Control Probe . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .2-1
Temperature Stabilization Periods . . . . . . . . . . . . . . . . . . . . . . . . . .2-1
Alarms . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .3-1
Probe Failure Alarm . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .3-2
High Stage System Failure . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .3-2
Section 4 Maintenance . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .4-1
Clean Air Filter . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .4-1
Clean Cabinet Exterior . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .4-1
Clean Condenser . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .4-1
Clean Water-cooled Condenser . . . . . . . . . . . . . . . . . . . . . . . . . . . .4-2
Defrost Chamber . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .4-3
Vacuum Relief Port . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .4-3
Clean Door Gasket . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .4-3
Prepare Unit for Storage . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .4-5
Replace Battery(s) . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .4-5
PREVENTIVE MAINTENANCE . . . . . . . . . . . . . . . . . . . . . . . . .4-6
Section 1
Section 2
Section 3
Section 4

vi TSE Series Thermo Scientific
Factory Installed Options . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .5-1
Back Up System (BUS) - P/N 1950445, 1950447 . . . . . . . . . . . . . .5-1
Install Vent Stack, Solenoid and Injection Assembly . . . . . . . . . . . .5-1
Install Temperature Probe . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .5-3
Connect Probe/Solenoid Harness . . . . . . . . . . . . . . . . . . . . . . . . . .5-4
BUS Control Panel . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .5-5
Set Optional BUS Set Point . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .5-6
Configure Optional BUS (Back-Up System) . . . . . . . . . . . . . . . . . .5-6
Test BUS Operation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .5-7
Disconnect Fitting Asm. and Transfer Hose . . . . . . . . . . . . . . . . . . .5-7
Clean Vent Stack . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .5-7
Chart Recorder . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .5-7
Recorder Calibration . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .5-8
Change Recorder Temperature Range . . . . . . . . . . . . . . . . . . . . . . .5-8
Installing the Chart Paper . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .5-8
Five Inner Door Option . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .5-9
Water-cooled Condenser . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .5-9
Datalogger . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .5-9
Specifications . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .6-1
Exploded Parts Drawings . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .7-1
Refrigeration Schematics . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .8-1
Electrical Schematics . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .9-1
Warranty . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .10-1
Handling Liquid Nitrogen . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .A-1
Handling Liquid CO2. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .B-1
First Aid . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .C-1
Table of Contents
Section 5
Section 6
Section 8
Section 7
Section 9
Section 10
Appendix

TSE Series 1-1Thermo Scientific
Section 1 Installation and Start-up
• Control panel - keypad, displays and indicators
• BUS (Optional Back Up System) control panel
• Optional temperature recorder or datalogger
Optional Back Up
System Controls
Temperature
Recorder
(Optional)
Control Panel
Figure 1-1. Freezer Front View Components

1-2 TSE Series Thermo Scientific
Section 1
Installation and Start-Up
Thermo Scientific
Optional Back-up
System Connections
Power Switch
(mains
disconnect)
Power Inlet
Remote Alarm
Contacts
Wall Bumper
(pre-tapped holes)
Figure 1-2. Freezer Rear View Components
Optional recorder /
datalogger probe
Optional PT1000 3m long -
calibration probe additional
Performance Alarm Monitor
and Temperature Probes.
PT100 sensor
Shelf
bracket
Control RTD/Redundant
Type T Thermocouple probe
Sensor sealant
CAUTION: This is critical seal.
Seal must be maintained.
Figure 1-3. Chamber Probe(s)
• Remote alarm contacts
• Power inlet for power cord connection
• Optional BUS connections for probe and solenoid
• Power switch (mains disconnect)

TSE Series 1-3Thermo Scientific
Section 1
Installation and Start-Up
• Vacuum relief port - pressure equalization port
• Probe cover - houses control, optional recorder, datalogger, or 1535
alarm probes
• Battery mounting bracket(s)
• Battery power switch (freezer and BUS)
• Freezer battery
• Optional BUS battery
• Freezer filter location
Figure 1-4. Vacuum Relief and Probe Cover Location
O
Battery power
switch To
remove filter
Thermocouple
interconnect jack
Battery mounting
bracket
Freezer battery
and optional BUS battery
Figure 1-5. Battery(s) location and switch

• Temperature Display - Displays temperature in degrees Celsius.
• Mode Select Switch - Used to select Run, Set Temperature, Set High
Alarm, Set Low Alarm, Calibrate, Backup.
• Alarm Indicator - Light pulses on/off during an alarm condition of the
cabinet.
• Silence - Silences the audible alarm. See Section 4 for alarm ringback
times.
• Alarm Panel - indicates the current alarm condition.
• Up and Down Arrows - Increases or decreases values, toggles between
choices.
• Enter - Stores the value into memory
1-4 TSE Series Thermo Scientific
Section 1
Installation and Start-Up
Temperature
Display
Mode
Key
Alarm
Indicator
Silence
Key
Alarm
Panel
Up and Down
Arrows
Enter Key
Figure 1-6. Control Panel
Control Panel Keys,
Displays, Indicators

The TSE Series freezer has five basic modes which allow freezer setup and
operation. Press the Mode key to scroll through the mode selections.
Up Arrow: Increases or toggles the parameter value.
Enter: Must press Enter key to save to memory all changed
values.
Down Arrow: Decreases or toggles the parameter value.
Silence Key: Press to silence the audible alarm. See Section
4 for alarm ringback times.
Note If tipped more than 45°, allow the unit to sit upright for 24 hours
before start up. s
To remove the freezer from the pallet, use a 7/16" wrench to remove all
the bolts securing the shipping bracket to the pallet.
Remove the shipping bracket. Remove the ramp boards from the pallet
and place the slotted end over the ramp brackets on the pallet. The
support blocks on the ramps will be facing down. Before moving the
freezer, make sure the casters are unlocked and moving freely. Align the
caster with the ramp boards. Use adequate personnel to roll the freezer off
the pallet.
The freezer can be easily pushed to the desired approved location,
described below. If necessary, the doors and lower front panel may be
opened to move the unit through tight openings. When the freezer is in
position, set the front caster brakes.
Note The freezer must not be moved with the product load inside. s
Note For proper ventilation and airflow, a minimum clearance of 5” at the
rear and top and a clearance of 8” on the side of the freezer is required.
Allow adequate space in the front of the freezer for door opening. s
Locate the freezer on a firm, level surface in an area with an ambient
temperature between 18°C and 32°C. Provide ample room to reach the
mains disconnect switch (power switch) located on the rear of the freezer.
TSE Series 1-5Thermo Scientific
Section 1
Installation and Start-Up
Install Freezer
Choose Location
Operation of the
Keypad

The parts bag, located inside the cabinet, contains the following parts.
Install the bolts into the pre-tapped holes on the back of the compressor
section. Install a neoprene cap on each bolt. Refer to Figure 1-2 for the
locations of the pre-tapped holes.
Install the shelf clips into the shelf pilasters (front and back) at the desired
shelf level. Install the shelves in the cabinet onto the clips.
Note Maximum shelf load is 100 lbs (45.4 kg) per shelf. s
Note On units having the optional 5 inner door option, refer to the
instructions accompanying the inner door kit. s
See Figure 1-2 for the location of the remote alarm contacts. The remote
alarm connector is located in the parts bag provided with the manual. It
must be installed if connecting the freezer to an alarm system. After
installing the wiring from the alarm system to the connector, install the
connector to the freezer microboard and secure with the two screws
provided. The remote alarm provides a NO (normally open) output, a NC
normally closed) output and COM (common). The contacts will trip on a
power outage and high temperature or low temperature alarms. They will
also trip on high stage, control probe or microboard failures.
Figure 1-8 shows the remote contacts in alarm state.
1-6 TSE Series Thermo Scientific
Section 1
Installation and Start-Up
Install Shelves
Remote Alarm Contacts
Figure 1-8. Remote Alarm Contacts
IMPORTANT USER INFORMATION
IMPORTANT USER INFORMATION
Caution! Stored product should be protected
by a redundant 24 hour/day monitoring
system with alarm capability. An interconnect
jack and thermocouple are installed
for centralized monitoring should on-board
system fail.
Quantity Stock # Description Purpose
2 510016 1/4-20 x 5-1/2” Bolt Wall Bumper
2 380520 Neoprene Cap Cap Protector
Table 1-1. Parts bag
Install Wall Bumpers

Insert the power cord into the power inlet module. Place the retaining
bracket (P/N 195763) over the connector. Tighten retaining screws to
secure.
See the serial tag on the side of the unit for electrical specifications or refer
to the electrical schematics in this manual. s
The freezer should be operated on a dedicated grounded service. Check
the voltage rating on the serial tag of the unit and compare it with the
outlet voltage. Then, with the power switch turned off, plug the line cord
into the wall outlet.
First, turn on the freezer power switch. Then open the lower front door by
grasping the bottom left corner. Locate the battery switch (Figure 1-5) and
turn it to Standby mode ( ). During initial freezer start-up, the system
battery may require charging and the Low Battery indicator may
illuminate.
Note Ensure the battery switch is turned to Standby mode ( ). The
rechargeable batteries require 36 hours to charge at initial start-up. A “Low
Battery” alarm may occur until the batteries are fully charged. Should a
power failure occur during the initial start-up period, the electronics will
have limited operation. s
TSE Series 1-7Thermo Scientific
Section 1
Installation and Start-Up
Attach Power Cord
Connect Unit to Electrical
Power
Figure 1-9. Power Cord Connection

With the freezer properly installed and connected to power, system set
points can be entered. The following set points can be entered in Settings
mode: Control temperature, high temperature alarm set point, low
temperature alarm set point, and (optional) BUS set point. Default settings
are shown in the table below.
Note If the set point is changed and the low temperature and high
temperature alarms are set 10° from the set point, the alarm set points will
be adjusted automatically to maintain a distance of at least 10° from set
point. s
TSE Series freezers have an operating temperature range of -50°C to -
86°C, depending on ambient temperature. The freezer is shipped from the
factory with a temperature set point of -80°C. To change the operating
temperature set point:
1. Press the Mode key until the Set Temperature indicator lights.
2. Press the up/down arrow key until the desired temperature set point is
displayed.
3. Press Enter to save the set point.
4. Press the Mode key until the Run indicator lights for Run mode
If no keys are pressed, the freezer will automatically return to RUN mode
after 5 minutes.
Note If the set point is changed and the low temperature and high
temperature alarms are set 10° from the set point, the alarm set points will
be adjusted automatically to maintain a distance of at least 10° from set
point. s
1-8 TSE Series Thermo Scientific
Section 1
Installation and Start-Up
Freezer Start-Up
Default Settings Temperature
Control Set Point -80°C
High Temperature Alarm -70°C
Low temperature alarm -90°C
Optional BUS Set Point -60°C
Table 1-2. Default Settings
Set Operating
Temperature

The high temperature alarm will activate an audible/visual warning when
the freezer chamber temperature has reached or exceeded the high
temperature alarm set point.
To set the high temperature alarm set point:
1. Press the Mode key until the Set High Alarm indicator lights.
2. Press the up or down arrow key until the desired high temperature
alarm set point is displayed.
3. Press Enter to save the setting.
4. Press the Mode key until the Run indicator lights for Run mode
If no control keys are pressed, the freezer will automatically return to RUN
mode after 5 minutes.
Note The high alarm set point must be set at least 5°C from the control
set point. s
Note At initial start-up, the high temperature alarm is disabled until the
cabinet reaches set point or 12 hours elapse. s
The low temperature alarm will activate an audible/visual warning when
the freezer chamber temperature has reached or decrease below the low
temperature alarm set point.
To set the low temperature alarm set point:
1. Press the Mode key until the Set Low Alarm indicator lights.
2. Press the up or down arrow key until the desired low temperature
alarm set point is displayed.
3. Press Enter to save the setting.
4. Press the Mode key until the Run indicator lights for Run mode
If no control keys are pressed, the freezer will automatically return to RUN
mode after 5 minutes.
Note The low alarm set point must be set at least 5°C from the control set
point. s
TSE Series 1-9Thermo Scientific
Section 1
Installation and Start-Up
Set High Temperature
Alarm
Set Low Temperature
Alarm

Run mode is the default mode for the freezer. This mode displays the
cabinet temperature on the temperature display under normal operating
conditions. In addition, the Run mode allows display of the high stage heat
exchange temperature.
This information scrolls by pressing the up or down arrow keys. The
display returns to the operating temperature in 10 seconds if no keys are
pressed.
1-10 TSE Series Thermo Scientific
Section 1
Installation and Start-Up
Run Mode

TSE Series 2-1Thermo Scientific
Section 2 Calibrate
Once the freezer has stabilized, the control probe may need to be
calibrated. Calibration frequency is dependent on use, ambient conditions
and accuracy required. A good laboratory practice would require at least an
annual calibration check. On new installations, all parameters should be
checked after the stabilization period.
Caution Before making any calibration or adjustments to the unit, it is
imperative that all reference instruments be properly calibrated. s
Plug a type T thermocouple reader into the receptacle located inside the
lower door (see Figure 1-5). Compare the control temperature set point to
the temperature of the measuring device.
1. Press the Mode key until the Calibrate indicator lights.
2. Press up/down arrow to match the display to calibrated instrument.
3. Press Enter to store calibration.
4. Press the Mode key to return to Run mode.
Startup - Allow 12 hours for the temperature in the cabinet to stabilize
before proceeding.
Already Operating - Allow at least 2 hours after the display reaches set
point for temperature to stabilize before proceeding.
During calibration, the temperature display will not be available. s
If no keys are pressed for approximately five minutes while in calibration
mode, the system will reset to Run mode.
Calibrate Control
Probe
Temperature Stabilization
Periods

TSE Series 3-1Thermo Scientific
Section 3 Alarms
The TSE Series freezer alarms are displayed on the freezer control panel.
When an alarm is active, the indicator next to the alarm description will
light and there will be an audible alarm. Press the Silence key to disable the
audible alarm for the ringback period. The visual alarm will continue until
the freezer returns to a normal condition. The alarms are momentary
alarms only. When an alarm condition occurs and then returns to normal,
the freezer automatically clears the alarm condition.
All alarm delays and ringback times are ±30 seconds.
* The automatic battery test runs immediately on power-up, then every 8 hours thereafter.
Description Delay Ringback Relay
Power Failure 1 min. 15 min. Yes
High Temperature Alarm 1 min. 15 min. Yes
Low Temperature Alarm 1 min. 15 min. Yes
Probe Failure see 3.2 1 min. 15 min. No
Door Open 1 min. 15 min. No
Wrong Power 0 min. none Yes
Low Battery* 1 min. 8 hours No
Low BUS Battery (optional) 1 min. 15 min. No
Hot Condenser 1 min. none No
High Stage Failure 0 min. 15 min. Yes
Micro Board Failure 0 min. 15 min. Yes
Table 3-1. Alarm Indicators