Thermo Scientific Finnpipette F2 User manual

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Part ofThermo Fisher Scientific
Finnpipette®F2
Single Channel
Variable & Fixed Volume
Multichannel
Instructions for Use
Bedienungsanleitung
Guide d´utilisation
Instruccions de uso
取扱説明書

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This product complies with the European Union Directive 98/79/EC, and it is marked with
CE-symbol. If the pipette is used according to this directive, the user shall read the additional
information at www.thermo.com/finnpipette or contact the manufacturer.
Product specifications are subject to change without prior notice. Finnpipette®and Finntip®
are registered trademarks of Thermo Fisher Scientific Oy.
Dieses Produkt entspricht der Richtlinie 98/79/EG der Europäischen Union und ist mit dem
CE-Symbol gekennzeichnet. Wird die Pipette entsprechend dieser Richtlinie verwendet,
muss der Anwender die zusätzlichen Informationen auf www.thermo.com/finnpipette
lesen oder den Hersteller kontaktieren.
Wir behalten uns das Recht auf unangekündigte Änderungen der Produktspezifikationen vor.
Finnpipette®und Finntip®sind eingetragene Warenzeichen der Fa. Thermo Fisher Scientific Oy.
Ce produit est conforme à la directive de l’Union européenne 98/79/CE et porte le marquage
CE. Si la pipette est utilisée selon cette directive, l’utilisateur est tenu de contacter le fabricant
ou de lire les informations supplémentaires données sur www.thermo.com/finnpipette.
Les spécifications du produit sont sujettes à modification sans avis préalable. Finnpipette®
et Finntip®sont des marques déposées de Thermo Fisher Scientific Oy.
Este producto cumple la Directiva de la Unión Europea 98/79/CE y presenta el símbolo CE. Si
la pipeta se usa de acuerdo con esta Directiva, el usuario debe leer la información adicional
presente en www.thermo.com/finnpipette o ponerse en contacto con el fabricante.
Las especificaciones del producto pueden cambiar sin previo aviso. Finnpipette®y Finntip®
son marcas registradas de Thermo Fisher Scientific Oy.
本製品は、欧州連合指令98/79/ECに準拠し、CEマークが表示されています。
ピペットをこの指令に準拠して使用する場合は、弊社ホームページ www.thermo.
com/finnpipette にある追加情報をお読みいただき、または弊社まで ご連絡くださ
い。製品仕様は事前の予告なく変更されることがあります。 フィンピペット®とフィン
チップ®は、サーモフィッシャーサイエンティフィックの登録商標です。

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CONTENTS
PRODUCT DESCRIPTION 4
PIPETTE OPERATION 5
PIPETTING TECHNIQUES 5
CALIBRATION AND ADJUSTMENT 6
MAINTENANCE 8
TROUBLE SHOOTING 11
PACKAGE 11
SPARE PARTS 49-55
English
INHALT
PRODUKTBESCHREIBUNG 12
PIPETTENFUNKTION 13
PIPETTIERMETHODEN 13
KALIBRIERUNG UND JUSTIERUNG 15
WARTUNG 17
FEHLERBEHEBUNG 20
PACKUNG 20
ERSATZTEILE UND ZUBEHÖR 49-55
Deutsch
SOMMAIRE
DESCRIPTION DU PRODUIT 21
UTILISATION DE LA PIPETTE 22
MéTHODES DE PIPETAGE 22
CALIBRAGE 24
ENTRETIEN 26
EN CAS DE PROBLEME 29
CONDITIONNEMENT 29
PIèCES DéTACHéES 49-55
Français
CONTENIDO
DESCRIPCIÓN DEL PRODUCTO 30
USO DE LA PIPETA 31
TéCNICAS DE PIPETEO 31
CALIBRACIÓN Y AJUSTE 33
MANTENIMIENTO 35
PAQUETE 38
SOLUCIÓN DE PROBLEMAS 38
PIEZAS DE RECAMBIO 49-55
Español
目次
製品について 39
ピペットの操作 40
ピペッティングテクニック 40
キャリブレーション 41
メンテナンス 43
トラブルシューティング 46
パッケージ 47
保証規定 47
部品及び付属品 49-55
日
本
語

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Product description
The Finnpipette F2 is a continuously adjustable, general purpose micropipette for sampling and
dispensing accurate liquid volumes.
It operates on an air displacement principle (i.e. an air interface) and uses detachable, disposable
tips.
The adjusted delivery volume is displayed digitally on a readout window in the handle.
The eleven different models of Finnpipette F2 pipettes cover a volume range from 0,2 µl to 10 ml.
Order No. Volume Range Finntip
4642010 0,2 µl to 2 µl Flex 10
4642020 0,5 µl to 5 µl Flex 10
4642030 1 µl to 10 µl Flex 10
4642040 1 µl to 10 µl 250 Universal, 200 Ext, Flex 300
4642050 2 µl to 20 µl 50
4642060 2 µl to 20 µl 250 Universal, 200 Ext, Flex 300
4642070 10 µl to 100 µl 250 Universal, 200 Ext, Flex 300
4642080 20 µl to 200 µl 250 Universal, 200 Ext, Flex 300
4642090 100 µl to 1000 µl 1000, Flex 1000
4642100 0,5 ml to 5 ml 5 ml
4642110 1 ml to 10 ml 10 ml, Flex 10 ml Ext
The thirteen different models of Finnpipette F2 Fixed Volume pipettes cover a volume range
from 1 µl to 10 ml.
Order No. Volume Range Finntip
4652000 1 µl Flex 10
4652010 5 µl Flex 10
4652020 10 µl 250 Universal, 200 Ext, Flex 300
4652030 25 µl 250 Universal, 300, 200 Ext, Flex 300
4652040 50 µl 250 Universal, 300, 200 Ext, Flex 300
4652050 100 µl 250 Universal, 300, 200 Ext, Flex 300
4652060 250 µl 1000, Flex 1000
4652070 500 µl 1000, Flex 1000
4652080 1000 µl 1000, Flex 1000
4652090 2000 µl 5 ml
4652100 3000 µl 5 ml
4652110 5000 µl 5 ml
4652120 10000 µl 10 ml
The ten different models of Finnpipette F2 Multichannel pipettes cover a volume range from
1 µl to 300 µl.
Order No. Channel Volume Range Finntip
4662000 8 1 µl to 10 µl Flex 10
4662010 8 5 µl to 50 µl 250 Universal, 200 Ext
4662020 8 10 µl to 100 µl 250 Universal, 200 Ext
4662030 8 30 µl to 300 µl 300, Flex 300
4662040 12 1 µl to 10 µl Flex 10
4662050 12 5 µl to 50 µl 250 Universal, 200 Ext
4662060 12 10 µl to 100 µl 250 Universal, 200 Ext
4662070 12 30 µl to 300 µl 300, Flex 300
4662080 16 1 µl to 10 µl Flex 10 (384)
4662090 16 5 µl to 50 µl 50
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Digital display
The adjusted delivery volume is clearly indicated in the large
digital display on the handle.
Raw materials
The Finnpipette F2 is made of mechanically durable and chemically resistant materials.
Description of tips
Finntips are recommended for use with the Finnpipette F2.
They are made of virgin natural colour polypropylene, generally regarded as the only
contamination free material suitable for tips. Finntips are also autoclavable (121°C).
Pipette operation
Setting the delivery volume
1. Set the delivery volume using the push button on the top of
the pipette. To increase the delivery volume, turn the push
button counterclockwise. To decrease the delivery volume,
turn it clockwise.
2. Make sure that the desired delivery volume clicks into place.
3. Do not set volumes outside the pipette´s specified volume range.
Using excessive force to turn the push button outside the range
may jam the mechanism and eventually damage the pipette.
Safety Label
You can mark the pipette application, your initials, the
calibration date, etc. on the safety label. Remove the old
label with a sharp needle. Mark the new label with a pencil
and slide the label back in place.
Tip ejection
To help eliminate the risk of contamination, each pipette
is fitted with a tip ejector system. To release the tip, point
the pipette at suitable waste receptacle and press the tip ejector with your thumb.
Pipetting techniques
Push and release the push button slowly at all times particularly when working with
high viscosity liquids. Never allow the push button to snap back.
Make sure that the tip is firmly attached to the tip cone. Check for foreign
particles in the tip.
Before you begin your actual pipetting work, fill and empty the tip 2-3 times with
the solution that you will be pipetting. Hold the pipette in an upright position while
aspirating liquid. The grippy should rest on your index finger. Make sure that the tips,
pipette and solution are at the same temperature.
Forward technique
Fill a clean reagent reservoir with the liquid to be dispensed.
1 Depress the push button to the first stop.
2. Dip the tip under the surface of the liquid in the
reservoir to a depth of about 1 cm and slowly
release the push button. Withdraw the tip from
the liquid touching it against the edge of the
reservoir to remove excess liquid.
3. Deliver the liquid by gently depressing the push
button to the first stop. After a delay of about
one second, continue to depress the push button
all the way to the second stop. This action will empty the tip.
4. Release the push button to the ready position. If necessary, change the tip and continue
pipetting.
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Reverse technique
The reverse technique is suitable for dispensing liquids that have a high viscosity or a tendency
to foam easily. The technique is also recommended for dispensing very small volumes. Fill a
clean reagent reservoir with the liquid to be dispensed.
1. Depress the push button all the way to the second stop.
2. Dip the tip under the surface of the liquid in the
reservoir to a depth of about 1 cm, and slowly
release the push button. This action will fill the
tip. Withdraw the tip from the liquid touching
it against the edge of the reservoir to remove
excess liquid.
3. Deliver the preset volume by gently depressing
the push button to the first stop. Hold the push
button at the first stop. Some liquid will remain
in the tip and this should not be included in
the delivery.
4. The remaining liquid should either be discarded with the tip or pipetted back into the
container.
Repetitive technique
The repetitive technique offers a rapid and simple procedure for repeated delivery of the same
volume. Fill a clean reagent reservoir with the liquid to be dispensed.
1. Depress the push button all the way to the second stop.
2. Dip the tip under the surface of the liquid in the
reservoir to a depth of about 1 cm, and slowly re
lease the push button. This action will fill the tip.
Withdraw the tipfrom the liquid touching against
the edge of the reservoir to remove excess liquid.
3. Deliver the preset volume by gently depressing
the push button to the first stop. Hold the push
button at the first stop. Some liquid will remain
in the tip and this should not be included in
the delivery.
4. Continue pipetting by repeating steps 2 and 3.
Pipetting of heterogeneous samples
(deproteinization in blood glucose determination, for example)
Use steps 1 and 2 of the forward technique to fill the tip with blood.
Wipe the tip carefully with a dry clean tissue.
1. Immerse the tip into the reagent and depress the
push button to the first stop, making sure the tip
is well below the surface.
2. Release the push button slowly to the ready
position.
This will fill the tip. Keep the tip in the solution.
3. Depress the push button to the first stop and
release slowly. Keep repeating this procedure
until the interior wall of the tip is clear.
4. Finally, depress the push button all the way to the second stop to completely empty the tip.
Calibration and adjustment
All Finnpipettes are factory calibrated and adjusted to give the volumes as specified with distilled
or deionized water using the forward pipetting technique. It should be noted that the use of other
pipetting techniques may affect the calibration results. The pipettes are constructed to permit
re-adjustment for other pipetting techniques or liquids of different temperature and viscosity.
Device requirements and test conditions
An analytical balance must be used. The scale graduation value of the balance should be chosen
according to the selected test volume of the pipette:
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Volume range readable graduation
under 10 µl 0.00 1 mg
10-100 µl 0.01 mg
above 100 µl 0.1 mg
Test liquid: Water, distilled or deionized, “grade 3” water conforming ISO 3696. Tests are done
in a draft-free room at a constant (±0.5°C) temperature of water, pipette and air between 15°C
to 30°C. The relative humidity must be above 50%. Especially with volumes under 50 µl the
air humidity should be as high as possible to reduce the effect of evaporation loss. Special
accessories, such as the evaporation trap, are recommended.
Procedure to check calibration
The pipette is checked with the maximum volume (nominal volume) and with the minimum volume.
A new tip is first pre-wetted 3-5 times and a series of ten pipettings is done with both volumes.
A pipette is always adjusted for delivery (Ex) of the selected volume. Use of forward pipetting
technique is recommended. The maximum permissible errors are designed for forward method.
Procedure:
1. Do 10 pipettings with the minimum volume.
2. Do 10 pipettings with the maximum volume.
3. Calculate the inaccuracy (A) and imprecision (cv) of both series.
4. Compare the results to the limits in the Table 1.
If the calculated results are within the selected limits, the adjustment of the pipette is correct.
TABLE 1: Maximum permissible errors according ISO8655
Range Volume Inaccuracy Imprecision
µl µl % s.d. µl cv%
0,2-2 µl 2 ±0.080 ±4 0.040 2.0
0.2 ±0.080 ±40 0.040 20.0
0,5-5 µl 5 ±0.125 ±2.5 0.075 1.5
0.5 ±0.125 ±25 0.075 15
1-10 µl 10 ±0.120 ±1.2 0.080 0.8
1 ±0.120 ±12 0.080 8.0
2-20 µl 20 ±0.20 ±1.0 0.10 0.5
2 ±0.20 ±10.0 0.10 5.0
10-100 µl 100 ±0.80 ±0.8 0.30 0.3
10 ±0.80 ±8.0 0.30 3.0
20-200 µl 200 ±1.60 ±0.8 0.60 0.3
20 ±1.60 ±8.0 0.60 3.0
100-1000 µl 1000 ±8.0 ±0.8 3.0 0.3
100 ±8.0 ±8.0 3.0 3.0
0,5-5 ml 5000 ±40.0 ±0.8 15.0 0.3
500 ±40.0 ±8.0 15.0 3.0
1-10 ml 10000 ±60.0 ±0.6 30.0 0.3
1000 ±60.0 ±6.0 30.0 3.0
Fixed Volume Inaccuracy Imprecision
µl µl % s.d.µl cv%
1 ±0.04 ±4.0 0.04 4.0
5 ±0.07 ±1.4 0.07 1.4
10 ±0.09 ±0.9 0.08 0.8
25 ±0.15 ±0.6 0.13 0.5
50 ±0.3 ±0.6 0.2 0.4
100 ±0.4 ±0.4 0.3 0.3
250 ±1.0 ±0.4 0.8 0.3
500 ±1.5 ±0.3 1.5 0.3
1000 ±3.0 ±0.3 0.3 0.3
2000 ±6.0 ±0.3 4.0 0.2
3000 ±9.0 ±0.3 6.0 0.2
5000 ±15.0 ±0.3 10.0 0.2
10000 ±30.0 ±0.3 20.0 0.2
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Range Channel Volume Inaccuracy Imprecision
µl µl % s.d.µl cv%
1-10 µl 8, 12, 16 10 ±0.24 ±2.4 0.16 1.6
1 ±0.24 ±24 0.16 16
5-50 µl 8, 12, 16 50 ±1.0 ±2.0 0.4 0.8
5 ±1.0 ±20 0.4 8.0
10-100 µl 8, 12 100 ±0.80 ±0.8 0.30 0.3
10 ±0.80 ±8.0 0.30 3.0
30-300 µl 8, 12 300 ±8.0 ±2.7 3.0 1.0
30 ±8.0 ±26.7 3.0 10.0
Adjustment
Adjustment is done with the service tool.
1. Place the service tool into the openings of the
calibration nut at the top of the handle.
2. Turn the service tool clockwise to increase, or
counterclockwise to decrease the volume.
3. After adjustment check the calibration according
to the instructions above.
Formulas for calculating results
Conversion of mass to volume
V = (w + e) x Z V = volume (µl)
w = weight (mg)
e = evaporation loss (mg)
Z = conversion factor for mg/µl conversion
Evaporation loss can be significant with low volumes. To determine mass loss, dispense water
to the weighing vessel, note the reading and start a stopwatch. See how much the reading
decreases during 30 seconds (e.g. 6 mg = 0.2 mg/s).
Compare this to the pipetting time from taring to reading. Typically pipetting time might be 10
seconds and the mass loss is 2 mg (10 s x 0.2 mg/s) in this example. If an evaporation trap or lid
on the vessel is used the correction of evaporation is usually unnecessary.
The factor Z is for converting the weight of the water to volume at test temperature and pressure.
A typical value is 1.0032 µl/mg at 22°C and 95 kPa. See the conversion table on page 48.
Inaccuracy (systematic error)
Inaccuracy is the difference between the dispensed volume and the selected volume of a pipette.
A = V - V0A = inaccuracy
V = mean volume
V
0= nominal volume
Inaccuracy can be expressed as a relative value: A% = 100% x A / V0
Imprecision (random error)
Imprecision refers to the repeatability of the pipettings. It is expressed as standard deviation (s)
or coefficient of variation (cv)
s = standards deviation
v = mean volume
n = number of measurements
Standard deviation can be expressed as a relative value (CV)
CV = 100% x S / V
Maintenance
When the Finnpipette F2 is not in use, make sure it is stored in an upright position. We
recommend a Finnpipette stand for this purpose.
The part # refer to exploded views beginning at page 49.
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Short-term service
The pipette should be checked at the beginning of each day for dust and dirt on the outside
surfaces of the pipette.
Particular attention should be paid to the tip cone. No other solvents except 70 % ethanol should
be used to clean the pipette.
Long-term service, single channel pipettes
If the pipette is used daily it should be checked every three months. The servicing procedure
starts with the disassembly of the pipette.
1-1000 µl pipettes
1. Press the tip ejector.
2. Rotate the tip ejector 11 counterclockwise and pull it out.
3. Turn out the tip cone counterclockwise with the service tool.
4. Pull out the piston and other parts. Push out with piston the rest of the
piston assembly. Then turn the tip cone upside down and tap all parts
from tip cone. Remember keep all parts in order on table for reassembly.
5. Clean the piston, the piston spring and the O-rings with a dry
napless cloth.
6. Check the tip cone for foreign particles.
7. Grease the cleaned parts with the lubricant that comes with the pipette.
8. Reassemble the pipette components.
0.2-2µl, 0.5-5µl & 1-10 µl: First, slide spring 22, o-ring support 23 and
o-ring 24 on the tube 21. With the 0.2-2µl model insert the tube 27 into
the tube 21. Then slide the spring 13, spring support 16 and tubes 17
and 18, bigger o-ring 19 and smaller o-ring 20 back on the piston.
Compress the spring with fingers by pressing piston and spring support
16 against each other and slide the tube 21 with rest of the parts on the
piston. Hold the spring compressed and carefully slide the entire
assembly into the tip cone and release the spring.
2-20 µl: Slide the spring 13, spring support 16 and tubes 17
and 18, bigger o-ring 19 and smaller o-ring 20 back on the
piston. Compress the spring with fingers by pressing piston
and spring support 16 against each other and slide the
bigger o-ring 19, smaller o-ring 20, spring support 21 and
the spring 22 (smaller diameter against spring support 21) on
the piston. Hold the spring compressed and carefully slide
the entire assembly into the tip cone and release the spring.
10-100 µl & 20-200 µl: Slide the spring 13,
spring support 16 and o-ring 17 back on the
piston. Slide the entire assembly into the
tip cone.
100-1000µl: Put the o-ring 17 and support ring
16 to the tip cone. Slide the spring 13 on the
piston and slide the entire assembly into the
tip cone.
9. All: Put the spring 15 and support 14 on top
of the tip cone and carefully insert the tip
cone assembly to the handle and turn it tight
by hand.
10. Reassemble the tip ejector.
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1-200µl 200-1000µl

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0.5-5ml & 1-10 ml pipettes
1. Press the tip ejector.
2. Rotate the tip ejector 10 counterclockwise to open it.
3. Disassemble the lower part of the tip ejector 14
(snap fitting).
4. Turn the cylinder 13 counterclockwise
and pull out the tip cone assembly.
5. Remove the cylinder 13 by pressing
the snaps fittings of the cylinder.
5. Clean and regrease the O-ring
12 and cylinder 13.
7. Assemble the parts in the opposite
order of disassembly.
Long-term service, multichannel pipettes
If the pipette is used daily it should be checked and lubricated
every three months.
1. Place the servico tool head#1 between ring 15 and ejector 23. Push the tool
until the partssnap from each other.
2. Check that the ejector lever is in up position and pull down the tip ejector part of the module.
Place the service tool head#2 in the hole of adapter tube 46.
3. Open the upper end of the tip ejector slightly and remove the tip ejector.
4. Screw out the module of the handle.
5. Pull out the module spring 19 and clip 22.
6. Press the spring 13 and remove the locking pieces 12 from the groove. Remove the
spring 13.
7. Take off the locking claws 44 and 45 and pull out the adapter tube and tube 43.
8. Use a screwdriver to remove the four screws in the module cover and lift off the cover.
9. Remove the piston bar and clean the pistons and tip cones with a dry nap–free cloth.
10. If needed, service the tip cones:
16-channel 1-10µl: The tip cones cannot be serviced, please replace if necessary.
30-300 µl, 10-100µl & 5-50 µl: Open the tip cone by carefully releasing the cover ring from
its snap joint with the screwdriver. Remove all the parts from the tip cone. Clean all the
parts. If needed, replace the o-rings. Take one piston. Slide the spring 33, cover ring 32
(larger hole), spring 34, support ring 35, (o–ring 37 bigger 5-50µl) and o–ring 36 (smaller)
onto the piston. Grease the o–ring with the lubricant provided in the pipette package. Slide
all the parts into the tip cone and close the snap joint of the cover ring.
1–10 µl: Open the tip cone by carefully releasing the cover ring from its snap joint with
the screwdriver. Remove all the parts from the tip cone. Clean all the parts. If needed,
replace the o-rings. Take one piston. Slide spring 33, cover ring 32 (larger hole), support 35,
o–ring 36 (bigger), o–ring 37 (smaller) and o–ring support 38 onto the piston. Then slide
spring 39, spring support 40 (sharp edges first) and o–ring 41 onto the o–ring sup port 38.
Grease the o–rings with the lubricant provided in the pipette package. Slide all the parts
into the tip cone and close the snap joint of the cover ring.
11. Install the piston bar with pistons and tip cones in the cover and close the cover with the
four screws. Insert the clip 22.
12. Place the adapter tube and tube 43 on the neck of the module and insert the locking claws
44 and 45. Insert the module spring 19.
13. Insert spring 13 and locking pieces 12 to the piston rod 16.
14. Place the tip ejector on the module. Push the spring 19 inside tip ejector parts and close
the upper end of the ejector and keep closed with fingers.
15. Screw the module in the handle and tighten with service tool head#2.
16. Push the tip ejector lever down, until you hear a “click”.
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