Thermo Scientific Orion AquaMate UV-Vis User manual

Thermo Scientific
Orion AquaMate
UV-Vis and Vis Spectrophotometers
269-289800 •Revision C •July 2015
User Manual

Table of Contents
Chapter 1.......................................................................................................................8
Spectrophotometer Introduction.................................................................................8
Spectrophotometer Overview......................................................................................................8
Packing List.................................................................................................................................9
Orion AquaMate User DocumentationCD.......................................................................10
Intended Use.............................................................................................................................10
Safety andSpecial Notices........................................................................................................11
Chapter 2.....................................................................................................................12
Spectrophotometer Basics........................................................................................12
Spectrophotometer Components...............................................................................................12
Instrument Connections...................................................................................................13
Instrument Keypad...........................................................................................................13
Vial Holder Carousels................................................................................................................15
Selecting andPositioning Vials and Cuvettes..................................................................16
Changing the Vial Holder Carousel..................................................................................17
Sample Positioner Setting forthe Vial Holder Carousel...................................................18
Chapter 3.....................................................................................................................20
Orion AquaMate Instrument Setup............................................................................20
Entering Parameter Values........................................................................................................20
Setting Utility Menu Options ......................................................................................................22
Printer Setting ..................................................................................................................22
Date/Time Setup Setting..................................................................................................24
Standby Setting................................................................................................................25
Baseline Expiration Setting..............................................................................................25
Beeper Setting .................................................................................................................26
Screen Contrast Setting...................................................................................................26
Calculator Function..........................................................................................................26
Managing Stored Tests..............................................................................................................27
Naming and Saving a Test...............................................................................................27
Loading Saved Test Files.................................................................................................29
Locking/Unlocking Tests using the Password..................................................................30
Deleting a Test.................................................................................................................30
Using the SmartStart Test Feature...................................................................................30
Chapter 4.....................................................................................................................32
Water Analysis Test Menu.........................................................................................32
Preprogrammed Methods..........................................................................................................32
Loading and Running a WaterAnalysisMethod on theAquaMate Instrument..........................33
Loading Test Methods from USB Stick to AquaMate Instrument.....................................34
Running a TestMethod....................................................................................................37
Running a Water Analysis Test Method...........................................................................38
Using the One Point Adjustment Feature..................................................................................40
Using theReverse Color Feature..............................................................................................42
Creating or Modifying a Test Method.........................................................................................44
How to Define theTest Method Parameters....................................................................44
How to Modify the Test Method Parameters....................................................................45

Chapter 5.....................................................................................................................46
Orion AQUAfast Reagent Chemistry Instructions for Orion AquaMate.................46
Orion AQUAfast Colorimetric Reagents Compatible with Orion AquaMate Instruments...........46
Orion AQUAfast Reagent Instructions.......................................................................................48
Recommendations for Avoiding Measurement Errors......................................................48
AC2002 Alkalinity-M (Alkalinity to pH 4.3) Tablet Test.....................................................49
AC3002P Alkalinity-P (Alkalinity to pH 8.2)Tablet Test...................................................50
AC2027 Aluminum Tablet Test ........................................................................................51
AC4P27 Aluminum Powder Pack & Liquid Test...............................................................52
AC2012 Ammonia Tablet Test.........................................................................................53
AC4P12 Ammonia Powder Pack Test..............................................................................54
ACR012 Ammonia Low Range Reaction Tube Test........................................................55
ACR011 Ammonia High Range Reaction TubeTest.......................................................56
AC2035 Bromine Tablet Test...........................................................................................57
AC2017 Chloride Tablet Test...........................................................................................59
AC2070 Chlorine (Free & Total) TabletTest....................................................................60
AC2071 Chlorine (Free) Tablet Test................................................................................62
AC2072 Chlorine (Total) Tablet Test................................................................................64
AC4P71 Chlorine (Free) Powder Pack Test.....................................................................66
AC4P72 Chlorine (Total) Powder Pack Test....................................................................67
AC3072 Chlorine (Total) High Range Tablet Test............................................................68
AC2099 Chlorine Dioxide Tablet Test..............................................................................69
CODL00 COD Low RangeDigestion Tube Test..............................................................71
CODH00 CODMid Range Digestion Tube Test..............................................................73
CODHP0 COD High Range Digestion Tube Test............................................................74
AC2029 Copper (Free & Total) Tablet Test.....................................................................75
AC4P29 Copper (Free) Powder Pack Test......................................................................76
AC2098 Cyanuric Acid Tablet Test..................................................................................77
AC2009 Fluoride SPADNS Liquid Test............................................................................78
AC3032T Hardness (Total) Tablet Test...........................................................................80
AC2030 Hydrazine PowderTest......................................................................................81
AC2078 Iron (II &III) Tablet Test.....................................................................................82
AC4P78 Iron (Ferro) PowderPack Test...........................................................................83
AC4P79 Iron (Total) Powder Pack Test...........................................................................84
AC2055 Manganese Tablet Test......................................................................................85
AC4P54 Manganese Low Range Powder Pack & Liquid Test.........................................86
AC4P55 Manganese High Range Powder Pack Test......................................................87
AC4P42 Molybdate Powder Pack Test............................................................................88
ACR007 Nitrate Reaction Tube Test................................................................................89
AC2046 Nitrite Tablet Test...............................................................................................90
AC4P46 Nitrite Powder Pack Test ...................................................................................91
ACD004 Nitrogen (Total) LowRange Digestion Tube Test..............................................92
ACD007 Nitrogen (Total) High Range Digestion Tube Test.............................................94
AC3048 Ozone Tablet Test..............................................................................................96
AC2001 pH Tablet Test....................................................................................................98
AC3001 pH Liquid Test....................................................................................................99
AC2095-WA Phosphate (Ortho) LowRange Tablet Test...............................................100
AC2096 Phosphate (Ortho) High Range Tablet Test.....................................................101
AC4P95 Phosphate (Ortho) Powder Pack Test.............................................................102
ACR095 Phosphate (Ortho) Reaction Tube Test...........................................................103

ACD095 Phosphate (Total)Digestion Tube Test...........................................................104
ACD095AH Phosphate (Acid Hydrolysable) Digestion Tube Test .................................106
AC2060 Silica Tablet Test..............................................................................................108
AC2061 Silica with Phosphate RemovalTablet Test.....................................................109
AC4P60 Silica Powder Pack Test..................................................................................110
AC4P82 Sulfate Powder Pack Test................................................................................111
AC2016 Sulfide Tablet Test...........................................................................................112
AC2065 Zinc Tablet Test................................................................................................113
Color Measurement from Application Log #131.............................................................114
UVA and UV254 Measurements from Application Log#137..........................................118
Chapter 6...................................................................................................................122
Standard Curve Test Menu......................................................................................122
Concentration Measurements using the Standard Curve Application (Custom Method).........122
Setting the Parameters fora Standard Curve................................................................123
Measuring Standards for a Standard Curve...................................................................124
Measuring Samples using a Standard Curve.................................................................126
Editing a Standard Curve...............................................................................................129
Selecting a Different Curve Fit for a Standard Curve.....................................................130
Recalling an Existing Standard Curve Method...............................................................131
Chapter 7...................................................................................................................132
Wavelength Scanning Test Menu............................................................................132
Setting the Parameters for a Scan.................................................................................133
Collecting a Baseline Scan and Scanning a Sample......................................................134
Viewing and Manipulating Scanned Data.......................................................................136
Recalling an Existing Scanning Method.........................................................................141
Chapter 8...................................................................................................................142
Absorbance, % Transmittance and Concentration Measurements......................142
Absorbance &% Transmittance Measurements......................................................................142
Using the Basic A-%T-C Test Method............................................................................142
Using the Advanced A-%T-C Test Method....................................................................144
Concentration Measurements..................................................................................................147
Using the Basic A-%T-C Test Method............................................................................147
Using the Advanced A-%T-C Test Method....................................................................150
Multiwavelength.......................................................................................................................154
Absorbance Ratio....................................................................................................................157
Absorbance Difference............................................................................................................159
3-Point Net...............................................................................................................................161
Kinetics....................................................................................................................................163
Chapter 9...................................................................................................................168
Performance Verification.........................................................................................168
Performance Verification Tests................................................................................................168
Accessing the Performance Verification Tests ........................................................................169
Troubleshooting Checklist..............................................................................................169
Wavelength Accuracy – Internal..............................................................................................170
Wavelength Accuracy – Standards..........................................................................................171
Photometric Accuracy..............................................................................................................173
Noise Measurement.................................................................................................................175
Stray Light................................................................................................................................176

Chapter 10.................................................................................................................178
Maintenance..............................................................................................................178
Routine Care............................................................................................................................179
Cleaning and Maintaining Vials and Cuvettes................................................................179
Cleaning the Windows of the Sample Compartment......................................................181
Changing the Fuse..................................................................................................................182
Replacing the Tungsten Lamp.................................................................................................184
Chapter 11.................................................................................................................186
Customer Services...................................................................................................186
Technical Support....................................................................................................................186
External Device Interfacing and Data Transfer........................................................................187
AquaMate Spectrophotometer Setup for Computer Interfacing .....................................187
USB Computer Cable Interfacing and USB Driver Installation.......................................188
Example Data Collection Program.................................................................................191
AquaMate Spectrophotometer Setup for Printer Interfacing...........................................195
USB Printer Cable Interfacing and Printing Data............................................................196
Instrument Specifications.........................................................................................................197
Ordering Information................................................................................................................199
Appendix A................................................................................................................202
General Instrument Information..............................................................................202
Parameters..............................................................................................................................202
Concentration Units........................................................................................................207
Calculations for Software.........................................................................................................209

8|Orion AquaMate Spectrophotometer User Manual Thermo Scientific
1
Spectrophotometer Introduction
Spectrophotometer Overview
Thermo Scientific™Orion™AquaMate™UV-Vis and Vis spectrophotometersoffer the following
features and benefits:
•Easy operation using the 260+ preprogrammed methods for common colorimetric reagents
•Flexibility tocreate newmethods for additional reagents or samples – create new methods
using calibration standards or update methods using published wavelengths and equations
•Use a variety of vial sizes with the included 3-position vial holder carousel and updatethe
vial position on the carousel with a key press – the sample changer keypad moves the
carousel to the desired location for easy vial switching
•Performance verification tests ensure wavelength accuracy and instrument functionality,
plus built-in filters allow for wavelength verification with noadditional equipment required
•Additional functions include standard curve concentration measurements, wavelength
scanning, multiple fixed-wavelength measurements, absorbance ratio anddifference
•One year instrument warranty
The Orion AquaMate 8000 UV-Vis spectrophotometer measures in the 190 nm to 1100 nm
wavelength range using a Xenon Flash lamp that requires no warm-up time and has a five-year
average lifespan.
The Orion AquaMate 7000 Vis spectrophotometermeasuresin the 325 to 1100 nm wavelength
range using a Tungsten lamp,designed foreasy replacement using the factory pre-aligned
lamp and base.
CHAPTER 1

Chapter1|Spectrophotometer Introduction
Thermo Scientific Orion AquaMate Spectrophotometer User Manual |9
Packing List
Orion AquaMate 8000 UV-Vis Spectrophotometer
•UV-Vis Spectrophotometer
•Preprogrammed Methods on USB memory stick, Catalog Number AQ0USB
•6-position vial holder carousel, Catalog Number AQ006C
•3-position vial holder carousel, Catalog Number AQ003C
•1-position vial holder carousel, Catalog Number AQ001C
•AquaMate user documentation CD
•Printed instrument test report
•Dust cover
•USB cable
•110V US/Japanpower cord, Catalog Number AQ110V
•220V Euro powercord, Catalog NumberAQ220V
•240 V UK/Singapore powercord, Catalog Number AQ240V
Orion AquaMate 7000 Vis Spectrophotometer
•Vis Spectrophotometer
•Preprogrammed Methods on USB memory stick, Catalog Number AQ0USB
•3-position vial holder carousel, Catalog Number AQ003C
•AquaMate user documentation CD
•Printed instrument test report
•Dust cover
•USB cable
•110V US/Japan power cord, Catalog Number AQ110V
•220V Euro powercord, Catalog NumberAQ220V
•240 V UK/Singapore powercord, Catalog Number AQ240V
Note: The China/Australia/New Zealand power cord, Catalog Number AQCHNV, must be
ordered separately.

Chapter1|Spectrophotometer Introduction
10 |Orion AquaMate Spectrophotometer User Manual Thermo Scientific
Orion AquaMate User Documentation CD
The Orion AquaMate user documentation CD includes the following:
•Orion AquaMate Vis and UV-Vis Spectrophotometer User Guide
•Orion AQUAfast Reagent Chemistry Instructions forOrion AquaMate Spectrophotometers
•Orion AquaMate Master Method List
•Orion AquaMate Site and Safety Information
•Orion AquaMate Getting Started Guide
•Orion AquaMate Declarationof Conformity
•Orion Laboratory Product Warranty Card
•USB Driver
Intended Use
Please read this user manual thoroughly. Any use outside of these instructions may invalidate
the instrument warranty andcause permanent damage to the instrument.

Chapter1|Spectrophotometer Introduction
Thermo Scientific Orion AquaMate Spectrophotometer User Manual |11
Safety and Special Notices
Make sure you follow theprecautionary statements presented inthis user manual. The safety
and other special notices appear in boxes.
Safety and special noticesinclude the following:
Note: Contains helpful supplementary information
Important: Instructions that must be followedto avoiddamaging system hardware or dataloss
Caution: Statements that indicate a hazardous situation that, if notavoided,could result in
minor or moderate injury
Warning: A hazardoussituation that, if notavoided,could result in death or serious injury
Thermo Fisher Scientific provides this document toits customers with a product purchase to
use in the product operation. The contents of this documentare subject to change without
notice. All technical information in this document is for reference purposes only. System
configurations and specifications in this document supersede all previous information received
by the purchaser.
Thermo Fisher Scientific makes no representations that thisdocument is complete, accurate or
error free and assumes no responsibility and willnot be liable for any errors, omissions,
damage or loss that might result fromany use of this document, even if the information in the
document is followed properly.
This document is not part of any sales contract between Thermo Fisher Scientific Inc. and a
purchaser. This document shall in no way govern or modify any terms andconditions of sale,
which terms and conditions of sale shall govern all conflicting information between the two
documents.
For Research Use Only. This instrument is not a medical device and isnot intended to be used
for the prevention, diagnosis, treatmentor cure of disease.
Warning: Avoid an explosion or fire hazard. Thisinstrumentis not designed for use in an
explosive atmosphere.

12 |Orion AquaMate Spectrophotometer User Manual Thermo Scientific
2
Spectrophotometer Basics
Spectrophotometer Components
The following are some of the major components visible on the outside of the instrument:
CHAPTER 2
USB Port
Keypad
Sample Compartment

Chapter2|Spectrophotometer Basics
Thermo Scientific Orion AquaMate Spectrophotometer User Manual |13
Instrument Connections
The followingconnectorsare on the back of theinstrument.
Warning: Avoid shock hazard. Always turn off the instrument and unplug it from the wall outlet
or power strip before you unplug the powercord from the instrument connector.
Instrument Keypad
USB Computer Port
USB Printer Port
AC Power Connector
On/Off Switch
Fuse Compartment

Chapter2|Spectrophotometer Basics
14 |Orion AquaMate Spectrophotometer User Manual Thermo Scientific
Keys
Function
Function keys - Press to perform the action shown above each key on the
instrument display. The function keys will change depending on the displayed
screen. Some function keys may not be active on the display.
Escape key - Press to clear a value being entered or return to a previous
screen.
Clear key - Press to clear the last character entered.
Enter key - Press to accept highlighted, entered or selected values or press to
advance to the next parameter or screen.
Numeric keys - Use to enter numbers, a decimal point or minus sign to make a
value negative.
Arrow keys - Press to move the location of the curser or press to highlight a
value or option from a list of selections.
Utility key - Press to access the utility menu and instrument setup options.
Test key - Press to access the test menu with list of available analysis modes.
Print key - Press to export results to a printer or computer (when PCis selected
in the utility menu).
Sample changer keys - Press to select the location of the vial to be measured.
B is the blank position and 1-5 are the sample positions when using the Auto 6
sample positioner setting and B is the blank position and 2 and 4 are the sample
positions when using the Auto 3 sample positioner setting.

Chapter2|Spectrophotometer Basics
Thermo Scientific Orion AquaMate Spectrophotometer User Manual |15
Vial Holder Carousels
The Orion AquaMate 8000 and Orion AquaMate 7000 instrumentsinclude the three-position
vial holder carousel preinstalled in the sample compartment. The three-position carousel has
one adjustable 13 to 25 mm round vial holder in position B, one 10 mm square vial holder in
position 2 and one20 to 50 mm (long path) rectangular vial holder in position 4.
The Orion AquaMate 8000 instrument also includes the six-position vial holdercarousel and the
one-position vial holder carousel for 10 mm square vials and traditional spectrophotometer
applications, allowing youto take advantage of thefull capabilities of the instrument.
AquaMate Three-Position Carousel, Catalog Number AQ003C:
Side View Top View
AquaMate Six-Position Carousel,
Catalog Number AQ006C:
AquaMate One-Position Carousel,
Catalog Number AQ001C:
A new one-position carousel that holds one 100mm rectangular vial can nowbe purchased
using Catalog Number AQ100C.
Warning: If the Sample Positioner option is set to Auto6, whenever the Run Test function key
is pressed to start a measurement, the instrument will initialize the sample positioner and then
run the blankin the B location on the sample positioner and measure five samples in the 1-5
locations on thesample positioner.

Chapter2|Spectrophotometer Basics
16 |Orion AquaMate Spectrophotometer User Manual Thermo Scientific
Selecting and Positioning Vials and Cuvettes
The compatible wavelength range for different types of vials and cuvettes depends on the
material used. Thepath length of test tubesis not aswell defined as that of square cuvettes.
Vial / Cuvette Type
Wavelength Range
Optical Glass
360 nm to > 1100 nm
Borosilicate Glass
330 nm to > 1100 nm
Quartz
190 nm to > 1100 nm
Disposable:
Polystyrene
> 340 nm
Methacrylate
> 300 nm
Acrylic
> 280 nm
UV-transparent
> 220 nm
Note: See the manufacturer’sspecifications and work within the recommended range.
Position vialsand cuvettes so that theclear sides face the light beam, one clear side facing the
front of the instrument and the other facing the back.
Note: Always placevials in the instrument in exactly the same orientation in the light beam. An
alignment mark on the vial helps orientation the vial consistently and correctly.
When using small aperture (small volume) cuvettes:
•Always used cuvettes with black masking
•Use the samecuvette foryour blankand your samples
Z-dimension
The figure belowillustrates the position ofthe light beam in the instrument. Beam size
specifications are shown below.
•Distance frombottom of the vial/cuvette to center of beam (Z-dimension): 8.5 mm
•Beam dimensions: 2 mm (wide) by 7mm (tall)

Chapter2|Spectrophotometer Basics
Thermo Scientific Orion AquaMate Spectrophotometer User Manual |17
Changing the Vial Holder Carousel
The Orion AquaMate 8000 and Orion AquaMate 7000 instrumentshave the three-positionvial
holder carousel preinstalled in the samplecompartment. Thiscarousel can bechanged to use
the six-position vial holder carousel or one-position vial holder carousel.
Installing the Six-Position or One-Position Vial Holder Carousel
1. Open the samplecompartment door andlet it reston its hinge.
2. Remove the preinstalled three-position vial holder carousel. Loosen the captive
thumbscrew andpull straight up on thecarousel to liftit out of the sample compartment.
3. With one hand, carefully lower the six-position or one-position carousel straight down into
the sample compartment.
4. With the other hand, tighten the captive thumbscrew or thumbscrews.
5. Close the sample compartment door.
Note: If the carousel is not aligned correctly, you will not be able to tighten the thumbscrews.
Six-Position Carousel
One-Position Carousel
Captive Thumbscrew
Captive Thumbscrews
Alignment Pin Holes

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18 |Orion AquaMate Spectrophotometer User Manual Thermo Scientific
Sample Positioner Setting for the Vial Holder Carousel
The instrument allows the use of various vial holder carousels to take measurements. When
selecting the test parameters, the Sample Positioner setting determines how the instrument will
work with the installed vial holder carousel to obtain readings in the test measurement screen.
The Sample Changer keys on the instrument keypad are also be used for certain settings.
Sample Positioner Settings
Setting
Description
Manual 3
This setting is only used in the Water Analysis test type.
Use the Sample Changer keys to position the carousel with the desired vial holder in the
beam pathway for measurement. Positions B, 2 and 4 are selectable.
Auto 6
Measure one blank and five samples without changing vials on the carousel.
The instrument automatically measures the blank and advances the carousel to measure
the remaining samples in positions 1 through 5.
Auto 3
Measure one blank and two samples without changing vials on the carousel.
The instrument automatically measures the blank and advances the carousel to measure
the samples in positions 2 and 4.
Manual 6
Measure the blank and up to five samples on the carousel.
Use the Sample Changer keys to position the carousel to the appropriate position for
sample measurement. Place the blank vial in the B position and samples in position 1-5.
Regardless of the carousel position, the carousel automatically goes to the B position and
measures the blank when the Measure Blank function key is pressed. Use the Sample
Changer keys to select different positions for samples.
1-Cell
Platform
Measure the blank and all samples using one position on the carousel.
Place the blank in the holder, measure the blank, remove the blank, place a sample in
the holder, measure the sample, remove the sample and repeat for all samples. The
Sample Changer keys do not function is this setting.
Note: When the carousel is installed and Manual 6 is selected, the instrument always considers
the vial in the B position as a blank. Thismeans thateven after measuring the blank the first
time, samplescan only be placed in positions 1 through 5.

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Thermo Scientific Orion AquaMate Spectrophotometer User Manual |19
Three-Position Vial Holder Carousel
Corresponding Sample Changer Keys
Initialization of the Vial Holder Carousel
When the Run Test function key is pressed tostart a measurement, the instrument will display
the message “Calibrating and Checking Turret, Please Wait” while it attempts toinitialize the
vial holder carousel (SamplePositioner) at the blank (B) position. With the AquaMatethree-
position carousel, the instrument will rotate the carousel to the default position and then rotate
the carousel back to the last selected measurement position (B, 2 or 4). This ensures that the
vial holderis properly positioned in the beam.
If a one-position vial holder carousel has been installed,the message “Error, Single Cell Holder
found. Use Single Cell Holder?” will appear. Press the Accept Change function key to continue
or install the AquaMate three-position carousel and press the Cancel Change function key.
If the six-position vial holder carousel hasbeen installed, the instrument will initialize to the
blank (B) position. After it has initializedthe carousel, the instrument will proceed to the test
measurement screen.
Note: To prevent a Fatal Error message when removing the vial holder carousel, always return
to the main menu or the test parametermenu before removing the carousel. If the carousel is
removed whilein the test measurement screen, the instrument will display ““Fatal Error: 8 Press
Esc to return to main menu.” If this message is displayed, press the esc key and then press the
Run Test function key to re-initialize the vial holder carousel.

20 |Orion AquaMate Spectrophotometer User Manual Thermo Scientific
3
Orion AquaMate Instrument Setup
Entering Parameter Values
The following instructions outline the use of the Orion AquaMate instrument keypad with various
menus, settings and controls.
Numeric Entry
Highlight the desired parameter (i.e. Wavelength) and start typing the numeric value. TheEntry
window will open with the range of the parameter shown below. Type thedesired value and
then press the enter key.
CHAPTER 3