Thermo Scientific GENESYS 30 User manual

GENESYS 30
Spectrophotometer
User Guide
269-317300 Revision A March 2015

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Thermo Scientific GENESYS 30 User Guide 1
GENESYS 30 User Guide
The Thermo Scientific™ GENESYS™ 30 visible spectrophotometer is designed to measure
transmittance and absorbance in the range 325 nm to 1100 nm. Typical samples are
contained in a square plastic or glass cuvette with a pathlength of 10 mm. Sample holders that
support measurement of solutions in test tubes or longer cuvettes, and limited options for
measuring transmitting solid samples, are also available.
The spectrophotometer incorporates control software capable of displaying absorbance or
transmittance data as single wavelength measurements or as a wavelength scan. It can also
perform simple mathematical operations on data obtained at one or multiple wavelengths and
present the results of these calculations. Additionally, the software supports quantitative
analyses using either a user-entered calibration factor or establishment of a calibration
equation using between one and six measured standard solutions.
Data from the spectrophotometer’s measurements are displayed on a high resolution color
screen (32 bit, 800 x 480 pixels) and may also be printed using an optional thermal printer
accessory or saved to a flash memory device through a USB port conveniently located on the
front of the instrument.
All aspects of the users’ interactions with the GENESYS 30 visible spectrophotometer have
been carefully considered and accommodated in the design of the instrument. Accessibility of
the sample compartment, layout and labeling of the keypad, and placement of the tools and
features in the software are all optimized to provide the best possible user experience for the
instrument user.

2GENESYS 30 User Guide Thermo Scientific
Table of Contents
•“Terminology” on page 3
•“About the Keypad” on page 4
•“About the Keypad” on page 4
•“Live Display” on page 8
•“Scan” on page 11
•“Quant” on page 13
•“Fixed” on page 17
•“OD600” on page 20
•“Library” on page 22
•“Settings” on page 23
•“SmartStart” on page 30
•“Optional Accessories” on page 31
•“Halogen Lamp” on page 35

Thermo Scientific GENESYS 30 User Guide 3
Terminology
Field An area on the screen is where you can enter a value.
Tool An icon on the screen where you can select something either to choose a
setting or to make something happen.
Navigate Press to move the focus between tools and fields on the
screen.
Focus The field or tool that is selected is described as being “in focus.” It is indicated
by an orange line around it.
Select To select a tool or field, navigate to it and press
Save Tool Some applications allow the user to save methods to an on-board method
library or to a USB memory device. Methods saved to a USB memory device
can be transfered to other GENESYS 30 instruments or archived on a PC.
The Scan, Fixed and Quant applications also support saving tables of data
directly to a USB memory device for off line storage or processing. When you
select the save tool, a dialog box will prompt you to choose whether to save
the method or the data and direct you through the necessary steps to name
methods before saving. Method files are saved with user-specified names.
Data files are automatically named as Scan_, Fixed_, or Quant_ followed by a
date and time stamp. Data files cannot be saved with a user-specified name.
Focus
Message bar - look here for
instructions and reports
Baseline status
indicator
Tools

4GENESYS 30 User Guide Thermo Scientific
About the Keypad
Important features of your software:
Press Autozero to record a blank value in Live Display, Fixed, OD600, Analyzer
and Quant modes. Record a baseline in Scan mode.
Press the Home key to return to the home page at any time. If going to the
home menu will cause you to lose result data, the software will warn you and
give you the opportunity to cancel the action before going to Home.
When the number in a field is highlighted you can simply start keying in
numbers and it will be over-written.
If there is a line cursor between or next to the value, you need to use to
erase digits one by one. Also, if you erase a value completely and then press
, the value that was in the cell before you erased it will be restored.
• In any text or number entry fields causes one character or number to
be erased per press.
• In alerts or dialogs where the user is not entering numeric or text data and
there is a Cancel button displayed on the screen, pressing on the
keypad has the same effect as navigating to Cancel and pressing .
Number pad: enter numeric
values
Nav keys: controls navigation
on the instrument screen
Autozero: measure a
blank value or
baseline scan
Home: go to the home screen
Stop: stop the
current operation
Start: start the measurement specified using
the current settings
Backspace: remove
displayed values for
new data entry
Print: print the method or results
to the on-board printer with a date
and time stamp
Enter: accept user-entered values

Thermo Scientific GENESYS 30 User Guide 5
About the Home page
In addition to starting measurements in data modes, the start button acts as
short-cut to load a method when in the Library.
Note: If you press the measure button when the blank/baseline is not valid the
software plays the unhappy tone through the speaker and flashes the blank
indicator three times.
Stop
Print
Enter
Options are clearly labeled with the name of the measurement modes or functions
that they execute. Use the nav keys to select the desired application and press to
start it.

6GENESYS 30 User Guide Thermo Scientific
This feature is not yet available. See Fixed for detailed information.
See OD600 for detailed information Load or transfer saved methods in the
on-board memory or an inserted USB
memory device. See Library for detailed
information.
See Live Display for detailed information. See Quant for detailed information.

Thermo Scientific GENESYS 30 User Guide 7
Access the various functional settings for
instrument behavior including connecting to
a computer running control software. See
Settings for detailed information.
See Scan for detailed information.
You may choose to save selected methods to
a special SmartStart™ menu which will be
displayed instead of the home screen at
start-up. This home screen option brings you
to that same SmartStart menu.

8GENESYS 30 User Guide Thermo Scientific
Live Display
Live Display shows a continuously updating value for the transmittance, absorbance or
concentration of a sample. The instrument takes a new measurement every two (2) seconds.
The value on the screen flashes to indicate that a new reading is being displayed.
%Transmittance or Absorbance Measurements
1. Select a measurement wavelength.
2. Select the Mode (%T or ABS).
3. Place a cuvette with a blank in the measurement position, close the lid and press .
4. Remove the blank cuvette, place the sample cuvette in the measurement position and
close the lid.
Live measurements begin automatically.
The %T or ABS value is displayed on the screen.
5. To make measurements of additional samples, simply put the cuvette with the new
sample in the measurement position, close the lid and wait for the displayed value to flash
to indicate that the measurement has been made.
Measurement mode
selection and indicator tool
Message bar - look here for
instructions and reports
Measured
ABS/%T value
Measurement wavelength
Baseline status
indicator
Tip If you are using the test tube holder you may not need to close the lid when
making measurements.