Thermo Scientific GENESYS 30 User manual

GENESYS 30
Spectrophotometer
User Guide
269-317300 Revision A March 2015

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Thermo Scientific GENESYS 30 User Guide 1
GENESYS 30 User Guide
The Thermo Scientific™ GENESYS™ 30 visible spectrophotometer is designed to measure
transmittance and absorbance in the range 325 nm to 1100 nm. Typical samples are
contained in a square plastic or glass cuvette with a pathlength of 10 mm. Sample holders that
support measurement of solutions in test tubes or longer cuvettes, and limited options for
measuring transmitting solid samples, are also available.
The spectrophotometer incorporates control software capable of displaying absorbance or
transmittance data as single wavelength measurements or as a wavelength scan. It can also
perform simple mathematical operations on data obtained at one or multiple wavelengths and
present the results of these calculations. Additionally, the software supports quantitative
analyses using either a user-entered calibration factor or establishment of a calibration
equation using between one and six measured standard solutions.
Data from the spectrophotometer’s measurements are displayed on a high resolution color
screen (32 bit, 800 x 480 pixels) and may also be printed using an optional thermal printer
accessory or saved to a flash memory device through a USB port conveniently located on the
front of the instrument.
All aspects of the users’ interactions with the GENESYS 30 visible spectrophotometer have
been carefully considered and accommodated in the design of the instrument. Accessibility of
the sample compartment, layout and labeling of the keypad, and placement of the tools and
features in the software are all optimized to provide the best possible user experience for the
instrument user.

2GENESYS 30 User Guide Thermo Scientific
Table of Contents
•“Terminology” on page 3
•“About the Keypad” on page 4
•“About the Keypad” on page 4
•“Live Display” on page 8
•“Scan” on page 11
•“Quant” on page 13
•“Fixed” on page 17
•“OD600” on page 20
•“Library” on page 22
•“Settings” on page 23
•“SmartStart” on page 30
•“Optional Accessories” on page 31
•“Halogen Lamp” on page 35

Thermo Scientific GENESYS 30 User Guide 3
Terminology
Field An area on the screen is where you can enter a value.
Tool An icon on the screen where you can select something either to choose a
setting or to make something happen.
Navigate Press to move the focus between tools and fields on the
screen.
Focus The field or tool that is selected is described as being “in focus.” It is indicated
by an orange line around it.
Select To select a tool or field, navigate to it and press
Save Tool Some applications allow the user to save methods to an on-board method
library or to a USB memory device. Methods saved to a USB memory device
can be transfered to other GENESYS 30 instruments or archived on a PC.
The Scan, Fixed and Quant applications also support saving tables of data
directly to a USB memory device for off line storage or processing. When you
select the save tool, a dialog box will prompt you to choose whether to save
the method or the data and direct you through the necessary steps to name
methods before saving. Method files are saved with user-specified names.
Data files are automatically named as Scan_, Fixed_, or Quant_ followed by a
date and time stamp. Data files cannot be saved with a user-specified name.
Focus
Message bar - look here for
instructions and reports
Baseline status
indicator
Tools

4GENESYS 30 User Guide Thermo Scientific
About the Keypad
Important features of your software:
Press Autozero to record a blank value in Live Display, Fixed, OD600, Analyzer
and Quant modes. Record a baseline in Scan mode.
Press the Home key to return to the home page at any time. If going to the
home menu will cause you to lose result data, the software will warn you and
give you the opportunity to cancel the action before going to Home.
When the number in a field is highlighted you can simply start keying in
numbers and it will be over-written.
If there is a line cursor between or next to the value, you need to use to
erase digits one by one. Also, if you erase a value completely and then press
, the value that was in the cell before you erased it will be restored.
• In any text or number entry fields causes one character or number to
be erased per press.
• In alerts or dialogs where the user is not entering numeric or text data and
there is a Cancel button displayed on the screen, pressing on the
keypad has the same effect as navigating to Cancel and pressing .
Number pad: enter numeric
values
Nav keys: controls navigation
on the instrument screen
Autozero: measure a
blank value or
baseline scan
Home: go to the home screen
Stop: stop the
current operation
Start: start the measurement specified using
the current settings
Backspace: remove
displayed values for
new data entry
Print: print the method or results
to the on-board printer with a date
and time stamp
Enter: accept user-entered values

Thermo Scientific GENESYS 30 User Guide 5
About the Home page
In addition to starting measurements in data modes, the start button acts as
short-cut to load a method when in the Library.
Note: If you press the measure button when the blank/baseline is not valid the
software plays the unhappy tone through the speaker and flashes the blank
indicator three times.
Stop
Print
Enter
Options are clearly labeled with the name of the measurement modes or functions
that they execute. Use the nav keys to select the desired application and press to
start it.

6GENESYS 30 User Guide Thermo Scientific
This feature is not yet available. See Fixed for detailed information.
See OD600 for detailed information Load or transfer saved methods in the
on-board memory or an inserted USB
memory device. See Library for detailed
information.
See Live Display for detailed information. See Quant for detailed information.

Thermo Scientific GENESYS 30 User Guide 7
Access the various functional settings for
instrument behavior including connecting to
a computer running control software. See
Settings for detailed information.
See Scan for detailed information.
You may choose to save selected methods to
a special SmartStart™ menu which will be
displayed instead of the home screen at
start-up. This home screen option brings you
to that same SmartStart menu.

8GENESYS 30 User Guide Thermo Scientific
Live Display
Live Display shows a continuously updating value for the transmittance, absorbance or
concentration of a sample. The instrument takes a new measurement every two (2) seconds.
The value on the screen flashes to indicate that a new reading is being displayed.
%Transmittance or Absorbance Measurements
1. Select a measurement wavelength.
2. Select the Mode (%T or ABS).
3. Place a cuvette with a blank in the measurement position, close the lid and press .
4. Remove the blank cuvette, place the sample cuvette in the measurement position and
close the lid.
Live measurements begin automatically.
The %T or ABS value is displayed on the screen.
5. To make measurements of additional samples, simply put the cuvette with the new
sample in the measurement position, close the lid and wait for the displayed value to flash
to indicate that the measurement has been made.
Measurement mode
selection and indicator tool
Message bar - look here for
instructions and reports
Measured
ABS/%T value
Measurement wavelength
Baseline status
indicator
Tip If you are using the test tube holder you may not need to close the lid when
making measurements.

Thermo Scientific GENESYS 30 User Guide 9
Concentration Measurements
Concentration measurements are based on measurement of a single standard solution of
known concentration. The instrument calculates a calibration based upon the absorbance of
the known standard and the assumption that there is zero absorbance at zero concentration. A
Factor is calculated from the data such that
Concentration = Factor x Absorbance
To perform a concentration measurement:
1. Select a measurement wavelength.
2. Set the Mode to C.
3. Select the units from the pop-up grid.
Follow the directions in the message bar.
4. Place a cuvette with a blank solution in the measurement position and press .
5. Type the standard concentration in the Std. Conc. box and press to confirm.
Set measurement
wavelength
Enter the conc. of the
standard
Select concentration units
for display
Select concentration mode
Absorbance of standard
solution Calculated factor

10 GENESYS 30 User Guide Thermo Scientific
6. Present and measure your standard as instructed in the message bar.
The software immediately begins live display of concentration and the message bar
prompts you to present your sample.
7. Remove the blank cuvette, place the sample cuvette in the measurement position, and
close the lid.
The Result field shows a live display of calculated concentration. Continue presenting
additional samples. The display updates every two seconds. The Result value flashes to
indicate that a new reading has been recorded.
Below the Result field, the software displays the standard concentration, the measured
absorbance of the standard, and the calculated factor.
8. Press to stop live measurements, clear the calibration, and re-measure the standard
solution.
To print the displayed value
If your instrument is equipped with a printer accessory, you can press to print the
currently displayed value with a time and date stamp. In concentration mode, the information
displayed below the Result field is also printed.
GENESYS 30 does not support saving Live Display methods to the library because the
parameters of the measurement are so simple.
Live display measurements are transient and are not stored in memory, so it is also not
possible to save Live Display data electronically. Users requiring a tabular record of absorbance
vs. time may opt to purchase VISIONlite™ software to run a kinetics experiment from an
attached Windows™ computer.

Thermo Scientific GENESYS 30 User Guide 11
Scan
To record a scan
1. Select the data mode, ABS or %T.
2. Enter the lower and upper scan limits (minimum scan range is 100 nm).
3. Place a cuvette with a blank in the measurement position, close the lid and press .
4. Remove the blank cuvette, place the sample cuvette in the measurement position, close
the lid and press .
The scan plot y-axis autoscales as the data is collected.
The current measurement wavelength and ABS/%T are displayed as the scan is recorded.
Measurement mode
selection and indicator tool
Scan upper wavelength limit
field
Scan lower wavelength limit
field
Toggle cursor on/off tool
(only active when a scan is
displayed)
Message bar - look here for
instructions and reports
Wavelength and ABS/%T
values at cursor position
Baseline status
indicator
Zoom tools
Clear tool
Save tool

12 GENESYS 30 User Guide Thermo Scientific
To analyze and store scan data
1. After the scan completes, the focus will move to the cursor tool .
2. Use the or to move the cursor line left and right.
Press once for a 1 nm increment, or hold to scroll.
The cursor position and corresponding ABS/%T value are displayed above the spectrum
plot.
3. Use to zoom in or out.
When zooming in, the screen centers on the cursor line.
4. Select to clear the plot area.
To save and print
Data files can only be saved to a USB memory device. The on-board library is exclusively for
Method files. Data files are named as Scan_<time and date> for easy identification and
include method information and a time and date stamp.
1. Select to save your data to a file on a USB memory device.
Data is saved as text files that can be opened by any computer spreadsheet or
word-processing application. Method files may be saved to the on-board Library or a
USB memory device. You can specify your own filename for a method.
For detailed information see the section on saving data and methods.
2. If your instrument is equipped with a printer accessory, you can print your results.
Select to print your data to the printer.
Printed data includes a time and date stamp.
See Optional Accessories for detailed information on installing and loading the optional
printer accessory.
3. Select to clear the scan plot.
You will be prompted to save your data before it is discarded.

Thermo Scientific GENESYS 30 User Guide 13
Quant
The GENESYS 30 Quant application supports up to six standard solutions and offers linear
fits with the option to force the line through zero.
1. Set the analytical wavelength.
a. Navigate to the Units tool and press .
b. Select the units from the pop-up grid.
2. Enter the concentrations of your standard solutions.
Any number of standards up to 6 is allowed.
3. Navigate to the Conc field for STD1.
Use the keypad to enter the concentration of STD1 and press to accept the value.
The focus moves to the field for STD2.
Clear tool
Save tool
Set the measurement
wavelength
Set the display unit
Select a linear data fit with a
non-zero intercept
Select a linear data fit forced
through zero
Message bar - look here for
instructions and reports
Baseline status
indicator

14 GENESYS 30 User Guide Thermo Scientific
4. Enter the concentrations for all your standards; navigate to and press .
Follow the directions displayed in the message bar.
5. Place a cuvette with a blank solution in the measurement position and press .
6. Present and measure your standards in order as instructed in the message bar.
After you measure the last standard, the focus will move to . Press to
accept the calibration data.
The screen moves to the Quant working screen.
Follow the instructions in the message bar to measure your samples.
The application supports a data table with entries for up to ten samples. Samples are labeled
S1 through S10. Once the data table is full you must clear it before you can measure
additional samples.
Tip If you wish to re-measure a standard, navigate back to the Conc field for that
standard and follow the directions in the message bar.
Tip You can save your method at any time by navigating to and selecting . If you
have measured all the standards listed in the table you may save the method as
calibrated or uncalibrated. If any of the standards has not yet been measured you can
only save the method as an uncalibrated method.
Tip Select to go back to the calibration screen and re-measure a standard if the
graph shows that your data does not represent a good enough fit.

Thermo Scientific GENESYS 30 User Guide 15
You can choose to view your data in any of three formats
Select to view or
print the calibration graph
and sample data table
Select to view
the calibration graph or
print the calibration graph
and standard data
Select to view
or print the results in table
format

16 GENESYS 30 User Guide Thermo Scientific
To save and print
GENESYS 30 provides the option to save your data directly to a USB memory device inserted
into the USB-A port on the front of the instrument. Data files are named as Quant_<time
and date> for easy identification and include method information and a time and date stamp.
1. Select to save your data to a file on a USB memory device.
Data is saved as text files that can be opened by any computer spreadsheet or
word-processing application. Method files may be saved to the on-board Library or a
USB memory device. You can specify your own filename for a method.
For detailed information see the section on saving data and methods.
2. If your instrument is equipped with a printer accessory, you can print your results.
Select to print your data to the printer.
Printed data includes a time and date stamp.
See Optional Accessories for detailed information on installing and loading the optional
printer accessory.
3. Select to clear the data table.
You will be prompted to save your data before it is discarded.

Thermo Scientific GENESYS 30 User Guide 17
Fixed
Fixed Mode
To run a fixed measurement
1. Select an Equation from the drop-down menu.
2. Set the measurement wavelength(s) and factor(s).
3. Select the Mode (ABS or %T).
4. Select the concentration Units.
5. Select .
6. Place a cuvette with a blank in the measurement position, close the lid and press .
Select either single
or multiple
wavelength
measurements
Toggles between
ABS and %T Select
concentration units to
be displayed

18 GENESYS 30 User Guide Thermo Scientific
7. Remove the blank cuvette, place the sample cuvette in the measurement position, close
the lid and press .
Tip
• Initially, six rows of sample data are displayed. On measuring the sixth sample an
additional four rows appear.
• Samples are labeled S1 through S10. Once the data table is full, save or print your
data, then clear the data table to measure additional samples.
Equation in use
Return to
setup screen
Clear tool
Save tool
Baseline status
indicator
Result values
Message bar - look here for
instructions and reports
Ten-sample
Table