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2. SAFETY INSTRUCTIONS
The autoclave has unique characteristics. Please read and understand
the operation instructions before first operation of the autoclave. The
following issues may require instructions guidance provided by the
manufacturer: how to operate the autoclave, the door safety
mechanism, the dangers involved in circumventing safety means, how
to ensure that the door is closed, and how to select a correct
sterilization program.
Make sure that you know where the main power switch is, where the
water cut-off valve is and where the compressed air disconnection
valves (if applicable) are located.
Autoclave maintenance is crucial for the correct and efficient function of
the device. We enclose a log booklet that includes maintenance
recommendations, with every device.
The weekly spore test is part of the preventive maintenance plan, along
with the annual validation of the sterilization processes that ensures
appropriate temperature dispersion within the chamber.
Never use the autoclave to sterilize corrosive products, such as: acids,
bases and phenols, volatile compounds or solutions such ethanol,
methanol or chloroform nor radioactive substances.
1. Never start using a new autoclave before the safety, licensing
and authorization department has approved it for use.
2. All autoclave users must receive training in proper usage from
an experienced employee. Every new employee must undergo a
training period under an experienced employee.
3. A written procedure must be established for autoclave operation,
including: daily safety tests, seal inspection and door hinge
inspection, smooth action of the closing mechanism, chamber
cleaning, prevention of clogging and preservation from corrosion,
what is permitted and what is prohibited for sterilization and
choosing a sterilization program.
4. Liquids may be sterilized only with the “liquids” programs. The
container must be covered but not sealed. Sealed bottles may
only be sterilized using a special program. The bottle must be
either Pyrex or a Borosilicate glass bottle. Verify that the two
temperature sensors are located inside two different bottles to
assure that the liquid temperature is 20°C below boiling
temperature for sealed bottles at the end of the cycle.
5. When sterilizing plastic materials, make sure that the item can
withstand sterilization temperature. Plastic that melts in the
chamber is liable to cause a great deal of damage.
6. Individual glass bottles may be placed within an appropriate
container that will be placed in a basket. Never place glass
bottles on the floor of the autoclave. Never fill more than 2/3 of
the bottle volume.
